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1.
Food Chem ; 403: 134391, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36182852

RESUMO

In this study, we qualitatively and quantitatively characterized anthocyanins from 61 different Lonicera caerulea L. genotypes harvested from northeast China and measured their reactive oxygen species (ROS) scavenging activity. Among the anthocyanin extracts obtained, nine anthocyanins were identified, and the anthocyanin compositions could be divided into three classes and seven subtypes according to their numbers and distribution. Furthermore, their total content by fresh weight was ranged from 158.44 mg/100 g to 1751.44 mg/100 g across all genotypes; their hydroxyl radical scavenging activity and superoxide anion scavenging activity ranged from 13.3 %-99.9 % and 21.5 %-82.8 %, respectively. We also deployed Grey Relational Analysis to determine which anthocyanin was the key antioxidant component in the nine anthocyanins. The relation between the ROS scavenging activity and the 9 anthocyanin content was established. Our results suggest cyanidin-3,5-diglucoside has the greatest correlation to the ROS scavenging activity of the anthocyanin extractions.


Assuntos
Antocianinas , Lonicera , Antocianinas/análise , Frutas/química , Espécies Reativas de Oxigênio , Antioxidantes/análise , Extratos Vegetais/farmacologia , Extratos Vegetais/análise
2.
J Environ Manage ; 325(Pt A): 116438, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36240641

RESUMO

In recent years, global warming has become an important topic of public concern. As one of the most promising carbon capture technologies, solid amine adsorbents have received a lot of attention because of their high adsorption capacity, excellent selectivity, and low energy cost, which is committed to sustainable development. The preparation methods and support materials can influence the thermal stability and adsorption capacity of solid amine adsorbents. As a supporting material, it needs to meet the requirements of high pore volume and abundant hydroxyl groups. Industrial and biomass waste are expected to be a novel and cheap raw material source, contributing both carbon dioxide capture and waste recycling. The applied range of solid amine adsorbents has been widened from flue gas to biogas and ambient air, which require different research focuses, including strengthening the selectivity of CO2 to CH4 or separating CO2 under the condition of the dilute concentration. Several kinetic or isotherm models have been adopted to describe the adsorption process of solid amine adsorbents, which select the pseudo-first order model, pseudo-second order model, and Langmuir isotherm model most commonly. Besides searching for novel materials from solid waste and widening the applicable gases, developing the dynamic adsorption and three-dimensional models can also be a promising direction to accelerate the development of this technology. The review has combed through the recent development and covered the shortages of previous review papers, expected to promote the industrial application of solid amine adsorbents.


Assuntos
Aminas , Dióxido de Carbono , Dióxido de Carbono/análise , Adsorção , Ar , Gases
3.
Clin Plast Surg ; 50(1): 71-80, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36396263

RESUMO

In this article, authors mainly introduce new digital technology in facial bone contouring surgery. In our experience, these new technologies are crucial in ensuring the satisfaction of surgical accuracy. Our previous studies have shown surgeons can use precise pre-operative design to reduce operative time, reduce bleeding during surgery. Additionally, augmented reality can enhance the perspective perception of surgeons combining virtuality and reality. What's more, robot-assisted surgical technology also has a strong application prospect in facial contouring surgery. In the future, the combination of soft tissue contouring surgery will make the facial bone contouring surgery safer and more effective.


Assuntos
Ossos Faciais , Procedimentos Ortopédicos , Humanos , Ossos Faciais/cirurgia , Estética , Face/cirurgia , Asiáticos
4.
Sci Total Environ ; 857(Pt 1): 159391, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36240915

RESUMO

Diatom-dominated biofilms and associated extracellular polymeric substances (EPS) may adapt to the stress of long-term exposure to nutrients and anthropogenic contaminants. However, such interactions in contaminated mangrove sediments have rarely been reported. Based on the in situ characterization of biofilm components and environmental factors, the present study aimed to explore the key factors involved in shaping sediment biofilms through correlational and multivariate analyses. The pennate diatom Navicula is the core taxon that plays a crucial role in balancing the abundance of Nitzschia and Cyclotella, and is the main producer of bound-polysaccharides. The taxa composition shifts in a high N/P matrix, with the populations of pennate diatoms increasing but that of centric diatoms decreasing. High nutrient concentrations yield more number of diatoms and elevated levels of EPS. Bacteria are the main consumers of EPS and tend to be more symbiotic with Nitzschia than the other two diatom taxa. Some bound-polysaccharides dominated by arabinose and glucose units are transformed into the colloidal fraction, whereas other conservative ones serve as structural materials in concert with the bound-proteins. The planktonic phase of Cyclotella breaks down the structural EPS secreted by pennate diatoms in a process that directly affects the dynamic renewal of benthic biofilms. Most heavy metals as well as bisphenol A inhibit the abundance of bacteria and diatoms but enhance most EPS fractions except bound-polysaccharides. The response of structural EPS to specific contaminants varies, exhibiting increases in Co and Ni levels but decreases in nonylphenol and methylparaben levels. The present study improves our understanding of the microbial carbon loop of benthic biofilms in mangrove ecosystems under stress by nutrients and mixed contaminants.


Assuntos
Diatomáceas , Ecossistema , Diatomáceas/fisiologia , Biofilmes , Bactérias , Polissacarídeos , Nutrientes
5.
Environ Res ; 216(Pt 2): 114602, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36265606

RESUMO

BACKGROUND: Although ozone exposure has neurological toxicity, it remains unclear whether it was associated with an increased risk of attention-deficit/hyperactivity disorders (ADHD) among childhood. METHODS: We matched the four-year average ozone concentration with questionnaire data for 35,103 children aged 3-12 years from seven cities in Liaoning, China, 2012-2013. Using mixed-effect logistic regression models, we assessed the association of ozone concentration with multiple ADHD indicators using the Conners Abbreviated Symptom Questionnaire (C-ASQ), including explicit attention-deficit/hyperactivity symptoms (ADHD; score ≥15), attention-deficit/hyperactivity disorder tendencies (ADHD-T; 11 ≤ score ≤14), and attention-deficit/hyperactivity problems (ADHP; score ≥11). Results were also stratified by sociodemongraphics. RESULTS: After adjusting for covariates, we found that each interquartile range (IQR) increase in ozone concentration was associated with an increased risk of ADHD, ADHD-T, and ADHP (P < 0.001) with an odds ratio of 1.12 (95% confidence interval, 1.04-1.21), 1.08 (1.03-1.13), and 1.09 (1.05-1.14), respectively. Additionally, we found greater effect estimates in children who reported longer exercise time (vs those with limited exercise time) with odds ratio of 1.18 (1.07-1.31) vs 1.06 (0.96-1.17) for ADHD, 1.13 (1.06-1.21) vs 1.03 (0.96-1.10) for ADHD-T, and 1.15 (1.08-1.21) vs 1.04 (0.98-1.10) for ADHP. Non-breastfed children were also shown to be more vulnerable to ADHD with an odds ratio of 1.22 (1.09-1.36) compared with 1.06 (0.96-1.16) among the rest. CONCLUSIONS: Long-term ozone exposure may be associated with increased ADHD among children. Additional studies are needed to validate our findings and support policies and interventions to address this growing public health concern.


Assuntos
Transtorno do Deficit de Atenção com Hiperatividade , Ozônio , Criança , Humanos , Transtorno do Deficit de Atenção com Hiperatividade/induzido quimicamente , Transtorno do Deficit de Atenção com Hiperatividade/epidemiologia , Razão de Chances , Inquéritos e Questionários , Atenção , Ozônio/toxicidade
6.
J Hazard Mater ; 442: 130013, 2023 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-36155297

RESUMO

Viruses transmitted by water have raised considerable concerns for public health. A novel memory photocatalyst of g-C3N4/WO3/biochar was successfully developed for effective inactivation of human adenoviruses (HAdVs) in water, in which WO3 as an electron-storage reservoir and biochar as an electron shuttle is employed to synergistically improve photocatalytic activity of g-C3N4. The tertiary composite exhibited continuous photocatalytic performance for HAdVs inactivation without regrowth in water under light-dark cycles, i.e., ∼3.9-log inactivation under 6-h visible light irradiation and an additional ∼1.1-log inactivation under the following 6-h dark. The enhanced virucidal mechanism was attributed to the heterojunction formation and especially the electron-transfer pathway switching via biochar incorporation, contributing to electron transfer and storage in the light phase and then electron release in the dark phase, along with obviously increased generation of the virus-killing •OH radicals under light-dark cycles.


Assuntos
Adenovírus Humanos , Humanos , Catálise , Desinfecção , Fotoperíodo , Água , Luz
7.
BMC Pulm Med ; 22(1): 117, 2022 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-35361163

RESUMO

BACKGROUND: To investigate the value of endobronchial ultrasound (EBUS) and virtual bronchoscopic navigation (VBN) combined with rapid on-site evaluation (ROSE) in diagnosing peripheral pulmonary lesions (PPLs). METHODS: Between January 1st 2019 to September 1st 2021, EBUS and VBN examination were performed in expected consecutive patients with PPLs who were admitted to Zhangzhou Affiliated Hospital of Fujian Medical University (Fujian, China). Finally, based on the calculation of expected diagnostic yield of R-EBUS biopsy and drop out, 198 eligible patients were randomly divided into ROSE group (100 cases) and non-ROSE group (98 cases). The diagnostic yield of brushing and biopsy, the complications, the procedure time, the diagnosis time and expense during diagnosis were analyzed. RESULTS: In the ROSE group, the positive rate of EBUS brushing and biopsy were 68%, 84%, respectively. The average procedure time and diagnosis time were 18.6 ± 6.8 min, 3.84 ± 4.28 days, respectively, and the average expense was 643.44 ± 706.56 US.$ (4093.15 ± 4494.67 yuan ¥). In the controls, the positive rate of brushing and biopsy were 44%, 74%, respectively. The average procedure time and diagnosis time were 15.4 ± 5.7 min, 6.46 ± 3.66 days, respectively. And the average expense during diagnosis was 1009.27 ± 713.89 US.$ (6420.28 ± 4541.33 yuan ¥). There was significant difference in the positive rate of EBUS brushing and biopsy, diagnosis time and expense during diagnosis between both groups. And no significant difference was observed in the complications and the procedure time. Additionally, the impact of ROSE on diagnostic yield in right upper lobe and the size of lesion ≤ 2 cm in diameter was significant. CONCLUSION: In combination with ROSE, EBUS could significantly improve the positive rate of diagnosing PPLs, shorten diagnosis time and reduce expense during diagnosis. ROSE will be of great importance in the diagnosis of PPLs and medical resource.


Assuntos
Neoplasias Pulmonares , Broncoscopia/métodos , Endossonografia/métodos , Humanos , Pulmão/diagnóstico por imagem , Pulmão/patologia , Neoplasias Pulmonares/diagnóstico por imagem , Neoplasias Pulmonares/patologia
8.
World J Clin Cases ; 10(30): 11139-11145, 2022 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-36338219

RESUMO

BACKGROUND: Perirectal epidermoid cysts are rare masses arising from the ectodermal germ cell layer of the hindgut and are predominantly found in middle-aged women. It is often difficult to make an accurate diagnosis of these cysts and it is equally challenging to distinguish it from other developmental cysts. CASE SUMMARY: We report the case of an 18-year-old female patient with a perirectal mass who presented to the hospital with constipation. The patient experienced sacrococcygeal falls and burns on the left buttocks during growth. Three-dimensional computed tomography scans indicated abnormal sacral vertebrae with the sacral canal partially enlarged and opened. Pelvic magnetic resonance imaging showed a 55 mm × 40 mm × 35 mm unilocular cystic mass in the perirectal space and a solitary sinus in the left ischiorectal fossa. The cyst was completely resected posteriorly using the sacrococcygeal approach. The pathology was verified to be an epidermoid cyst. The patient remained recurrence-free after 6 mo of follow-up. CONCLUSION: Successful treatment of perirectal epidermoid cysts depends on comprehensive evaluation. This is significant for the surgical approach and prognosis.

9.
World J Pediatr ; 2022 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-36355326

RESUMO

BACKGROUND: Despite extensive research evaluating the association between prenatal exposure to secondhand smoke (SHS) and the development of autism spectrum disorders (ASD), no study has investigated the association by considering the pre-conceptional period. This study aimed to investigate the associations of pre-conceptional and prenatal SHS exposure and the development of ASD among toddlers. METHODS: In this cross-sectional study, parents of 6049 toddlers aged 16-30 months were recruited from 7 tertiary hospitals, 21 communities, and 7 kindergartens located in seven cities in six provinces from five geographical regions of China. We analyzed the associations of SHS exposure and the odds of ASD among toddlers in different exposure windows (pre-conceptional and/or prenatal periods). Data were analyzed from November 2021 to January 2022. RESULTS: Among the 6049 toddlers included in the analysis [22.7 (4.1) months; 44.8% girls], 71 were identified and diagnosed with ASD. Compared with the unexposed toddlers, toddlers with pre-conceptional SHS exposure had higher odds of ASD (OR 2.30, 95% CI 1.36-3.84), while we observed a non-significantly positive association regarding prenatal SHS exposure. When considering both pre-conceptional and prenatal periods, toddlers who were continuously exposed to SHS during these two periods had higher odds than those without SHS exposure (OR 2.32, 95% CI 1.24-4.14). CONCLUSION: We reported positive SHS-ASD associations when exposed during the pre-conceptional period and continuously exposed during pre-conceptional and prenatal periods, emphasizing the critical window of pre-conception for targeted intervention on smoking.

10.
Front Psychiatry ; 13: 996971, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36424988

RESUMO

Background: Although intimate partner violence (IPV) against women is a public health issue around the world, there is a lack of evidence regarding the impact of maternal IPV on preschool children's health-related quality of life (HRQOL). Therefore, the aim of this study was to investigate the association between maternal IPV and HRQOL among Chinese preschool children, as well as the mediating role of maternal parenting styles. Methods: A cross-sectional study was conducted with 4,243 mother-child dyads who attended preschools. Mothers self-reported their parenting styles and experience of IPV. Children's HRQOL was collected through mother-proxy report with the Pediatric Quality of Life Inventory version 4.0 (PedsQL 4.0). Multivariate linear regression analysis was conducted to evaluate the association between maternal IPV and children's HRQOL. Mediation models were further applied to explore the possible mediating role of maternal parenting styles. Results: Of the included mothers, 7.4% had experience of IPV. Compared to children of mothers without any IPV exposure, those of mothers with experience of IPV had significantly lower scores in all HRQOL dimensions and summary scales. After adjustment for covariates, maternal IPV was significantly associated with children's lower physical health summary score, psychosocial health summary score, and total scale score. Mediation analysis showed that both rejection and overprotection mediated such associations, but not for the emotional warmth. Conclusion: Our findings indicated the need to screen maternal IPV supplemented with targeted interventions focusing on parenting styles, in order to mitigate the negative impact of maternal IPV on children's HRQOL.

11.
Gene ; : 147051, 2022 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-36427678

RESUMO

BACKGROUND: Since the discovery of the Proprotein Convertase Subtilisin/Kexin Type 9(PCSK9) gene has been involved in regulating low-density lipoprotein metabolism and cardiovascular disease (CVD), many therapeutic strategies directly targeting PCSK9 have been introduced. PCSK9 gain of function (GoF) mutations are associated with autosomal dominant hypercholesterolemia (ADH) and premature atherosclerosis. In contrast, PCSK9 loss of function (LOF) mutations have cardioprotective effects and can lead to familial hypo cholesterol in some instances. However, its potential impacts beyond the typical effects on lipid metabolism have not been elucidated. Therefore the study aimed to identify and verify PCSK9's possible effects beyond its traditional role in lipid metabolism. METHODS: The S127R is a PCSK9 gain of function mutation. Firstly, We used the data of the gene expression Omnibus(GEO) database to identify the differentially expressed genes between S127R mutation carriers and ordinary people. Secondly, the identification and analysis of significant genes were performed with various bioinformatics programs. Thirdly, to verify the possible effect and the potential pathways of PCSK9 on angiogenesis, we constructed PCSK9 low and high expression models by transfecting PCSK9-siRNA (small interfering RNA) and PCSK9-plasmid complex into human umbilical vein endothelial cells (HUVECs), respectively. Furthermore, Wound-Healing Assay and Capillary tube formation assay were applied to measure the effect of PCSK9 on angiogenesis. Fourthly, the expression level of VEGFR2 and the significant genes between PCSK9 low and high expression models were verified by quantitative real-time PCR. All data were analysed by GraphPad Prism 8 software. RESULTS: 88 DEGs were identified, including 45 up-regulated and 43 down-regulated DEGs. Furthermore, we identified the six genes (MMP9, CASP3, EGR1, NGFR, LEFTY1 and NODAL) as significantly different genes between PCSK9-S127R and Control hiPSC. Further, we found that these significant difference genes were mainly associated with angiogenesis after enrichment analysis. To verify the possible effect of PCSK9 on angiogenesis, we constructed low and high-expression PCSK9 models by transfecting siRNA and PCSK9-plasmid complex into human umbilical vein endothelial cells (HUVECs), respectively. The tubule formation test and Wound healing assays showed that overexpression of PCSK9 had an inhibitory effect on angiogenesis, which could be reversed by decreasing the expression of PCSK9. Moreover, bioinformatics analysis indicated that the six hub genes (MMP9, CASP3, EGR1, NGFR, LEFTY1 and NODAL) might play a vital role in the biological function of PCSK9 in angiogenesis. Real-time quantitative PCR was applied to clarify the expression profiles of these critical genes in overexpression/knockdown PCSK9. Finally, the expression levels of MMP9, Caspase3, LEFTY1, and NODAL were suppressed by overexpression of PCSK9 and could be alleviated by PCSK9 knockdown. Otherwise, EGR1 had the opposite expression trend, and there was no specific trend of NGFR after repeated experiments. CONCLUSION: PCSK9 might play an essential role in angiogenesis, unlike its typical role in lipid metabolism, and MMP9, Caspase3, LEFTY1, NODAL, and EGR1 may be involved in the regulation of angiogenesis as critical genes.

12.
Int J Infect Dis ; 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36400375

RESUMO

OBJECTIVES: To evaluate the possibility of using cerebrospinal fluid ubiquitin C-terminal hydrolase L1 (CSF UCH-L1), glial fibrillary acidic protein (GFAP), and neurofilament light protein (NF-L) for the diagnosis of neurosyphilis (NS). METHODS: A cross-sectional study of 576 subjects was conducted at Zhongshan Hospital from January 2021 to August 2022, to evaluate the diagnostic accuracy of CSF UCH-L1, GFAP, and NF-L for NS and analyse their correlations with CSF rapid plasma reagin (RPR), white blood cells (WBC), and protein. RESULTS: NS patients had higher CSF UCH-L1, GFAP, and NF-L levels than syphilis/non-NS and non-syphilis patients. Using a cut-off point of 652.25 pg/mL, 548.89 pg/mL, and 48.38 pg/mL, CSF UCH-L1, GFAP, and NF-L had a sensitivity of 85.11%, 76.60%, and 82.98% with a specificity of 92.22%, 85.56%, and 91.11%, respectively, for NS diagnosis. Moreover, parallel and serial testing algorithms improved their sensitivity and specificity to 93.62% and 98.89%, respectively. Interestingly, levels between CSF RPR-positive and-negative NS patients did not differ and showed weak or moderate correlation with WBC and CSF protein in syphilis patients. CONCLUSION: CSF UCH-L1, GFAP, and NF-L can be used as novel markers for the diagnosis of NS, independent of CSF RPR, WBC, and proteins.

13.
Artigo em Inglês | MEDLINE | ID: mdl-36418644

RESUMO

PURPOSE: To investigate the independent impact of threat-related and deprivation-related adverse childhood experiences (ACEs) on depressive symptoms among middle-aged and older adults, and to evaluate the moderating role of current economic status in these associations. METHODS: This cross-sectional study included 11,048 participants aged ≥ 45 years from the China Health and Retirement Longitudinal Study. We captured five threat-related ACEs and five deprivation-related ACEs by questionnaires. Depressive symptoms were assessed using the 10-item Centre for Epidemiological Studies Depression Scale. Current economic status was reflected by annual per capita household consumption expenditure. We performed logistic regression analyses to evaluate the independent association of childhood threat and deprivation with depressive symptoms, and conducted stratified analyses and tests for interaction to explore the moderation effect of current economic status in such associations. RESULTS: Compared with the nonexposed group, the experience of both childhood threat and deprivation were independently associated with greater risks of depressive symptoms later in life (odds ratio [OR] 1.75, 95% CI 1.49-2.05 for ≥ 2 threat-related ACEs; OR 2.02, 95% CI 1.67-2.43 for ≥ 2 deprivation-related ACEs). High current economic status significantly ameliorated the impact of childhood deprivation, but not threat, on depressive symptoms (P value for interaction 0.038). CONCLUSIONS: Both threat-related and deprivation-related ACEs were associated with the risk of depressive symptoms among middle-aged and older adults, while current economic status was a significant moderator in such risks only for childhood deprivation. The findings implied that prioritising targeted interventions for individuals with ACEs, especially for childhood deprivation victims who were economically disadvantaged in adulthood, may help mitigate depressive symptoms in later life.

14.
Psychophysiology ; : e14220, 2022 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-36416584

RESUMO

Fluctuation patterns of physiological response after acute stress might indicate one's coping potential with stress, yet this relationship has not been widely examined. The present study investigated how Locus of Control (LOC) can influence cortisol and HR responses in an uncontrollable stressful situation. Seventy-three participants were exposed to acute stress through the Trier Social Stress Test (TSST) after finishing the LOC measures. Multilevel regression results suggested that a curvilinear function best captured the shape of the cortisol trajectories which was further moderated by the level of internal LOC. Moreover, higher internality was associated with greater cortisol reactivity and faster recovery. Our study reveals the link between internal control and endocrine stress response. Finally, cortisol upregulation was discussed, as well as the internality-related accelerated recovery of cortisol, which may be involved in the effect of motivation arousal for better adaptation.

15.
J Oncol ; 2022: 7680657, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36405245

RESUMO

Immune-related genes and long noncoding RNAs (lncRNAs) have a significant impact on the prognostic value and immunotherapeutic response of uveal melanoma (UM). Therefore, we tried to develop a prognostic model on the basis of irlncRNAs for predicting prognosis and response on immunotherapy of UM patients. We identified 1,664 immune-related genes and 2,216 immune-related lncRNAs (irlncRNAs) and structured a prognostic model with 3 prognostic irlncRNAs by co-expression analysis, univariable Cox, LASSO, and multivariate Cox regression analyses. The Kaplan-Meier analysis indicated that patients in the high-risk group had a shorter survival time than patients in the low-risk group. The ROC curves demonstrated the high sensitivity and specificity of the signature for survival prediction, and the one-, three-, and five-year AUC values, respectively, were 0.974, 0.929, and 0.941 in the entire set. Cox regression analysis, C-index, DCA, PCA analysis, and nomogram were also applied to assess the validity and accuracy of the risk model. The GO and KEGG enrichment analyses indicated that this signature is significantly related to immune-related pathways and molecules. Finally, we investigated the immunological characteristics and immunotherapy of the model and identified various novel potential compounds in the model for UM. In summary, we constructed a new model on the basis of irlncRNAs that can accurately predict prognosis and response on immunotherapy of UM patients, which may provide valuable clinical applications in antitumor immunotherapy.

16.
Front Microbiol ; 13: 975406, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36406409

RESUMO

The impairment of pelvic floor muscle functions and Lactobacillus-deficient vaginal microbiota is common in postpartum women. However, few studies have explored the correlation between pelvic floor muscle functions and vaginal microbiota. Given this research gap, our study aims to investigate any potential association between these two conditions of postpartum women (6-8 weeks after childbirth). A total of 230 women who required postpartum pelvic floor function examination at Peking University International Hospital from December 2021 to April 2022 were enrolled in this study. The collected questionnaire information included progestational weight, body mass index (BMI), weight gain during pregnancy, neonatal weight, delivery type, multiparity, postpartum time, and urinary incontinence (UI). A total of 187 samples of vaginal secretions were collected, and the vaginal microbiota was detected by 16S rRNA sequence analysis. Finally, 183 samples were analyzed in the trial. All individuals were divided into two groups according to the results of pelvic floor muscle assessment to explore the difference between the incidence of postpartum urinary incontinence and vaginal microbiota. We found that the prevalence of UI was higher in the group with weakened pelvic floor muscles. Vaginal delivery, overweight, age, neonatal weight, and weight gain during pregnancy were all risk factors for postpartum urinary incontinence. The vaginal microbiome was no longer Lactobacillus dominant of most postpartum women (91.8%), while the diversity of microbiota increased. The Lactobacillus-deficient community, commonly labeled as community state type (CST) IV, was sub-divided into four communities. The abundance of vaginal Lactobacillus decreased in the group with compromised pelvic muscle functions, while the species richness and diversity increased significantly. In conclusion, the decreased pelvic floor muscle functions of postpartum women 6-8 weeks after delivery may disrupt the balance of vaginal microbiota, and the restoration of pelvic floor functions may contribute to a healthy and balanced vaginal microbiota.

17.
J Cell Mol Med ; 26(22): 5565-5579, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-36317423

RESUMO

Ferroptosis is a type of regulated cell death (RCD), and it plays an important role in the occurrence of diseases, especially the development of tumors. Ferroptosis of tumor cells affects the antitumor immunity and the immune response to treatment to varying degrees. Ferroptosis also plays a key role in immune cells. This review outlines the mechanism of the immune-related effects of ferroptosis pathways in tumor progression and treatment, and it discusses potential methods for improving antitumor immunity and enhancing the efficacy of current cancer treatments by targeting ferroptosis.


Assuntos
Ferroptose , Neoplasias , Humanos , Peroxidação de Lipídeos , Neoplasias/patologia
18.
Genet Res (Camb) ; 2022: 1500768, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36325267

RESUMO

Objective: Insulin resistance (IR) plays a key role in gestational diabetes mellitus (GDM) pathogenesis. The antiaging protein klotho has been proven to be closely related to IR. The purpose of this study was to investigate the effect of klotho on IR in GDM trophoblast cells. Methods: The GDM cell model of HTR-8/SVneo cells was induced by high glucose (HG). Plasmid transfection was used to mediate the overexpression or silencing of klotho. The effects of klotho on cell viability, IR, and the IGF-1/PI3K pathways were observed by RT-qPCR, western blot, Cell Counting Kit-8 detection, glucose uptake assay, and immunofluorescence detection. Results: Klotho expression was up-regulated in HG-induced cells. Overexpression of klotho could reduce the cell viability, insulin signaling molecules (INSR-α, INSR-ß, IRS1, IRS2, and GLUT4), and glucose uptake in HTR-8/SVneo cells of the HG group. In addition, the overexpression of klotho inhibited the levels of IGF-1, IGF-1R/p-IGF-1R, and the phosphorylation and activation of the signal transduction molecules PI3K/Akt/mTOR. On the contrary, klotho deletions could reverse these changes of HTR-8/SVneo cells induced by HG. Conclusion. In a word, the results of this study showed that the regulation of klotho played an important role in the IR of trophoblast cells induced by HG, which was mediated at least in part by the IGF-1/PI3K/Akt/mTOR pathway.


Assuntos
Diabetes Gestacional , Resistência à Insulina , Feminino , Humanos , Gravidez , Diabetes Gestacional/genética , Glucose/metabolismo , Resistência à Insulina/genética , Fator de Crescimento Insulin-Like I/genética , Fator de Crescimento Insulin-Like I/metabolismo , Fosfatidilinositol 3-Quinases/genética , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , Serina-Treonina Quinases TOR/metabolismo , Trofoblastos/metabolismo , Trofoblastos/patologia , Regulação para Cima/genética , Proteínas Klotho/metabolismo
19.
Toxicol Ind Health ; 38(12): 810-818, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36325635

RESUMO

Alveolar macrophages play a vital role in the development of acute silicosis, but the dynamic changes of M1 and/or M2 phenotypes have not been elucidated. In this study, acute silicosis models of rat were established by a one-time dusting method, and the rats were sacrificed after 1, 3, 7, 14, and 28 days. The polarity states of macrophages were assessed by measuring the M1/M2 marker genes of alveolar macrophages and the M1/M2 marker proteins in bronchoalveolar lavage fluid. The pathological changes of lung tissues were examined with hematoxylin and Eosin and Masson's trichrome staining. Our results showed that in the early stages, alveolar macrophages were mainly polarized into M1; with time, the polarization of M2 gradually became dominant. Microscopic sections showed significant pathological responses of inflammation and fibrosis. This work suggested that the alteration of alveolar macrophage polarization was involved in the lung pathologic responses to acute silicosis.


Assuntos
Macrófagos Alveolares , Silicose , Ratos , Animais , Macrófagos , Inflamação , Líquido da Lavagem Broncoalveolar
20.
Chem Biodivers ; 19(11): e202200459, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36328758

RESUMO

Turpiniae Folium, the dried leaves of Turpinia arguta Seem., is a kind of historic traditional Chinese medicine. Here, based on our previous study, we extracted the Turpiniae Folium polysaccharides (TFP) and isolated three polysaccharide fractions from TFP. Then, TFP and one of the major polysaccharide fractions (TFP-1a) were identified through HPLC, HPGPC, and ATR-FTIR. Furthermore, the evaluations of their antioxidative, anti-inflammatory activities and inhibitory effect on angiotensin II-induced vascular smooth muscle cells (VSCMs) proliferation in vitro were conducted. Both TFP and TFP-1a showed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe2+ chelating activities, and exerted strong anti-inflammatory activity. Moreover, TFP and TFP-1a also possessed a strong inhibitory effect on Ang II-induced VSCMs proliferation. On these premises, we inferred that TFP and TFP-1a could be potential and promising natural antioxidants, anti-inflammatory agents, and implicated to treat cardiovascular disease.


Assuntos
Antioxidantes , Músculo Liso Vascular , Antioxidantes/farmacologia , Polissacarídeos/farmacologia , Anti-Inflamatórios/farmacologia , Folhas de Planta
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