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1.
Ann Palliat Med ; 10(5): 5580-5589, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-34107706

RESUMO

BACKGROUND: Vitamin D deficiency is considered to be a global health problem. The purpose of this study was to evaluate the prevalence of vitamin D deficiency and analyze its related factors among adult residents in Hainan, a tropical island province of southern China. METHODS: A total of 1,700 healthy adults, aged 18-86 years (617 men and 1,073 women), were enrolled in our cross-sectional descriptive study. Binomial logistic regression analyses were performed to identify possible predictors of vitamin D status. RESULTS: The average serum 25-hydroxyvitamin D [25(OH)D] concentration was 37.66±10.77 ng/mL (males 43.60±11.8 ng/mL, females 34.20±8.40 ng/mL; I<0.001). The proportions of vitamin D sufficiency [25(OH)D ≥30 ng/mL], insufficiency [20 ng/mL ≤25(OH)D <30 ng/mL], and deficiency [25(OH)D <20 ng/mL] were 76.6%, 20.5%, and 2.9%, respectively. Vitamin D deficiency in the young, middle-aged, and elderly groups were 4.2%, 2.7%, and 1.7%, respectively. Vitamin D sufficiency was found to be positively associated with male sex (P<0.0001), age >40 years (P=0.014), habitation in a rural area (P<0.0001), summer/autumn seasons (P<0.0001), and having <13 years of formal education (P<0.0001). CONCLUSIONS: Our study was the first to assess the vitamin D status and analyze related factors among adult residents in Hainan Province, China. We found that vitamin D deficiency has low prevalence in this population, suggesting that before developing a strategy for the clinical use of vitamin D supplements in a region, the levels of vitamin D in generally healthy populations of that region should be assessed, to avoid unnecessary supplementation.


Assuntos
Deficiência de Vitamina D , Adulto , Idoso , China/epidemiologia , Estudos Transversais , Suplementos Nutricionais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Deficiência de Vitamina D/epidemiologia
2.
Sheng Wu Gong Cheng Xue Bao ; 37(5): 1619-1636, 2021 May 25.
Artigo em Chinês | MEDLINE | ID: mdl-34085446

RESUMO

As a typical food safety industrial model strain, Bacillus subtilis has been widely used in the field of metabolic engineering due to its non-pathogenicity, strong ability of extracellular protein secretion and no obvious codon preference. In recent years, with the rapid development of molecular biology and genetic engineering technology, a variety of research strategies and tools have been used to construct B. subtilis chassis cells for efficient synthesis of biological products. This review introduces the research progress of B. subtilis from the aspects of promoter engineering, gene editing, genetic circuit, cofactor engineering and pathway enzyme assembly. Then, we also summarized the application of B. subtilis in the production of biological products. Finally, the future research directions of B. subtilis are prospected.


Assuntos
Bacillus subtilis , Engenharia Metabólica , Bacillus subtilis/genética , Proteínas de Bactérias/genética , Edição de Genes , Regiões Promotoras Genéticas
3.
Chem Soc Rev ; 50(10): 6042-6093, 2021 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-34027943

RESUMO

Transformation of biomass to chemicals and fuels is a long-term goal in both science and industry. However, high cost is one of the major obstacles to the industrialization of this sustainable technology. Thus, developing catalysts with high activity and low-cost is of great importance for biomass conversion. The last two decades have witnessed the increasing achievement of the use of earth-abundant 3d-transition-metals in catalysis due to their low-cost, high efficiency and excellent stability. Here, we aim to review the fast development and recent advances of 3d-metal-based catalysts including Cu, Fe, Co, Ni and Mn in lignocellulosic biomass conversion. Moreover, present research trends and invigorating perspectives on future development are given.

4.
Food Res Int ; 143: 110298, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33992318

RESUMO

Numerous studies on bacterial growth or survival predictive models have been conducted since the establishment of predictive microbiology. However, limited research focused on the prediction of bacteria-producing enterotoxins, which are often the causative agents of food-borne diseases. This study aimed to determine an appropriate kinetic model of staphylococcal enterotoxin A (SEA) production in milk after contamination with Staphylococcus aureus. An S. aureus strain producing SEA was inoculated into milk with an initial inoculum concentration of approximately 3.0 log CFU/mL. All samples were incubated for 30-48 h at 20 °C ± 1 °C, 28 °C ± 1 °C, and 36 °C ± 1 °C separately under shaking or static conditions. Duplicate samples were carried out at appropriate intervals to count the number of S. aureus colonies and detect the concentration of SEA. Experimental results showed that the SEA concentration curves under all experimental conditions were sigmoidal and consisted of three phases: lag, exponential, and stationary. Thus, the modified Gompertz model was used to describe the profile of SEA concentration in milk during the incubation. A good fitting accuracy (R2 > 0.97) indicated that the modified Gompertz model was appropriate. In addition to temperature, shaking during incubation also affected the maximal production rate of SEA and the maximal SEA concentrations, and shortened the lag phase at lower incubation temperatures, suggesting that the mechanical movements (e.g., stirring, pumping, and flowing) during the milk processing would increase the risk of SEA occurrence. Besides, the time to detection (TTD) of SEA was found to range from 3 to 24.5 h at temperatures of 36 °C ± 1 °C-20 °C ± 1 °C, at which time the concentrations of S. aureus ranging from 5.0 log CFU/mL-6.9 log CFU/mL at the TTD. This study contributed to understanding the kinetics of SEA production and the possible factors affecting the synthesis of SEA during the manufacturing of liquid foods, such as milk.

5.
Clin EEG Neurosci ; : 15500594211018535, 2021 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-34028297

RESUMO

Continuous electroencephalography (cEEG) and quantitative analysis of EEG (qEEG) are used in various circumstances such as detecting seizures, identifying acute or delayed cerebral ischemia, monitoring sedative therapy, or assessing prognosis. The authors report 2 cases: (1) Case #1 was a patient with unilateral cerebral edema and uncal herniation with asymmetric cEEG and qEEG changes detected an hour before clinical examination changes were noted and (2) Case #2 was a patient with diffuse cerebral edema and trans-tentorial herniation with symmetric cEEG and qEEG changes detected an hour before clinical examination changes were noted. These cases demonstrate the ability of cEEG and qEEG in early detection of different types of cerebral herniation. qEEG can be utilized by intensive care unit (ICU) staff not trained in EEG interpretation as a surveillance method to detect cerebral herniation, which may provide an opportunity for early intervention in high-risk patients.

6.
Analyst ; 2021 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-34048512

RESUMO

Matrix metalloproteinase-1 (MMP-1) is associated with many types of cancers, including oral, colorectal, and brain cancers. This paper describes the fabrication of an MMP-1 immunosensor based on a gold nanoparticle/polyethyleneimine/reduced graphene oxide (AuNP/PEI/rGO)-modified disposable screen-printed electrode (SPE). A microwave-assisted single-step method was employed for the simultaneous reduction of gold and graphene oxide in a PEI environment to avoid AuNP agglomeration. The crystal structure, chemical composition, optical properties, and interior morphology of the materials were probed by X-ray diffraction, Raman spectroscopy, UV-visible spectrometry, and transmission electron microscopy techniques. To assemble a label-free MMP-1 immunosensor layer-by-layer, 3-mercaptopropionic acid was utilized due to its strong sulfur-gold bonding ability, and its tail end was attached to a carboxyl group, allowing the MMP-1 antibody (anti-MMP-1) to be subsequently cross-linked using the traditional N-(3-dimethylaminopropyl) and N' ethylcarbodiimide hydrochloride method. Differential pulse voltammetry analysis showed a linear relationship with MMP-1 concentration in the range of 1-50 ng ml-1 with an R2 value of ∼0.996 (n = 5, RSD < 5%). This immunosensor was successfully applied for MMP-1 detection in urine, saliva, bovine serum, and cell culture media (HSC-3 & C6) of oral and brain cancers showing results comparable to those of the credible ELISA method.

7.
Artigo em Inglês | MEDLINE | ID: mdl-33807938

RESUMO

Given the growing awareness of sustainable development, the environmental protection industry has attracted much attention. Green finance has developed rapidly in policymaking and practices. This study provides a framework for evaluating green finance via linkage analysis based on input-output theory. Measurements on industrial linkages are calculated in China in two provinces from 2002 to 2018, which study the relationship between finance and environmental protection sectors. The results show that the environmental protection sector (EPS) in China has gradually developed from a sector with weak backward and strong forward linkages to a sector with strong backward and weak forward linkages from 2002 to 2015; however, in 2017 and 2018, the EPS returned to a sector with weak backward and strong forward linkages. At the provincial level, the EPS used to be a key sector with strong backward and forward linkages. The connection between the finance sector and the EPS rose first, then declined in the country and the Zhejiang province; Guangdong had a similar evolution in the former period, but it had a rising trend in the latest year. The findings provide insights for further promoting the support from the finance sector to the environmental protection activities.


Assuntos
Conservação dos Recursos Naturais , Indústrias , China , Desenvolvimento Sustentável
8.
Ann Palliat Med ; 10(5): 5908-5918, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33894738

RESUMO

BACKGROUND: Recent studies show similar outcomes between lysis of adhesions (LOA) and manipulation under anaesthesia (MUA) in patients with arthrofibrosis within 3 to 6 months after arthroscopic knee procedures. As MUA offers positive efficacy with less expense and more convenience, the first consideration in clinical practice shifting to MUA may save much medical cost when MUA is non-inferior to surgery. There is a lack of evidence comparing the clinical outcomes of these two surgical techniques. The purpose of our study is to determine whether MUA is non-inferior to LOA for improving clinical outcomes in patients with loss of flexion within 3 and 6 months after arthroscopic knee surgery. METHODS: This multicentre randomized controlled trial (RCT) will recruit 360 patients within 3 to 6 months after arthroscopic knee surgery with a magnetic resonance imaging (MRI)-confirmed arthrofibrosis to prove non-inferiority of MUA. Patients are randomly assigned to the LOA group (n=180) or the MUA group (n=180) in a 1:1 ratio with random sequences using the SPSS Statistics V.22. The standard surgical procedures will be performed within 2 weeks after randomization, and each patient will receive unified perioperative physical therapy (PT). The primary outcome measure is the range of knee flexion. Secondary outcome measures include physical function, activity level, general health, knee pain, and the occurrence of adverse events. Safety measures include physiological parameters (i.e., blood pressure, pulse, respiratory rate) and complications (i.e., component damage, patellar tendon tear and fracture during surgical procedures, neurological or internal medicine conditions, venous thromboembolism, bleeding and infection postoperatively). The noninferiority margin is defined as a difference of 10 degrees on the range of knee flexion between groups and was assessed with a one-sided α of .025. To investigate relative effectiveness between the groups, linear mixed-effects model will be used for continuous data, and generalised estimating equation for dichotomous data. Analyses will follow the intention-to-treat principle. DISCUSSION: Findings of our study will help clinicians determine the risk-benefit balance of MUA and LOA, two frequently used surgical procedures for patients with knee arthrofibrosis. TRIAL REGISTRATION: Chinese Clinical Trial Registry (www.chictr.org.cn); ChiCTR2000033467.


Assuntos
Anestesia , Artropatias , Artroscopia , Humanos , Articulação do Joelho/cirurgia , Estudos Multicêntricos como Assunto , Ensaios Clínicos Controlados Aleatórios como Assunto , Amplitude de Movimento Articular , Resultado do Tratamento
9.
Chemistry ; 2021 Apr 19.
Artigo em Inglês | MEDLINE | ID: mdl-33871120

RESUMO

Members of a new class of complexes, 2(CF3 ), 2(H), 2(Br), 2(I), and 2(OCH3 ), have been synthesized in a one-pot method involving the treatment of osmanaphthalynes bearing corresponding substituents (1(CF3 ), 1(H), 1(Br), 1(I), and 1(OCH3 )) with trimethylphosphine (PMe3 ) and water. The main reaction process involves two steps, namely a ligand-exchange with trimethylphosphine and nucleophilic addition of water to the Os≡C bond of the osmanaphthalyne. The substituents have a significant influence on the rate of the reaction, as befits a nucleophilic addition. Fortunately, the key intermediate [1(OCH3 )]' could be successfully captured, and the detailed reaction mechanism has been explored with the aid of density functional theory (DFT) calculations, which were in excellent agreement with the experimental findings. All of the target complexes have been fully characterized by 1 H, 31 P{1 H}, and 13 C{1 H} NMR spectroscopy, high-resolution mass spectrometry, and elemental analysis.

11.
Sci Rep ; 11(1): 6062, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33723361

RESUMO

Men and women are sexually dimorphic but whether common anthropometric and biochemical parameters predict type 2 diabetes (T2D) in different ways has not been well studied. Here we recruit 1579 participants in Hainan Province, China, and group them by sex. We compared the prediction power of common parameters of T2D in two sexes by association, regression, and Receiver Operating Characteristic (ROC) analysis. HbA1c is associated with FPG stronger in women than in men and the regression coefficient is higher, consistent with higher prediction power for T2D. Age, waist circumference, BMI, systolic and diastolic blood pressure, triglyceride levels, total cholesterol, LDL, HDL, fasting insulin, and proinsulin levels all predict T2D better in women. Except for diastolic blood pressure, all parameters associate or tend to associate with FPG stronger in women than in men. Except for diastolic blood pressure and fasting proinsulin, all parameters associate or tend to associate with HbA1c stronger in women than in men. Except for fasting proinsulin and HDL, the regression coefficients of all parameters with FPG and HbA1c were higher in women than in men. Together, by the above anthropometric and biochemical measures, T2D is more readily predicted in women than men, suggesting the importance of sex-based subgroup analysis in T2D research.

12.
Theranostics ; 11(9): 4078-4089, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33754049

RESUMO

Inflammatory cytokines produced by muscularis macrophages largely contribute to the pathological signs of postoperative ileus (POI). Electroacupuncture (EA) can suppress inflammation, mainly or partly via activation of vagal efferent. The goal of this study was to investigate the mechanisms by which EA stimulation at an hindlimb region ameliorates inflammation in POI. Methods: Intestinal motility and inflammation were examined after 24 h after intestinal manipulation (IM)-induced POI in mice. Local immune response in the intestinal muscularis, expression of macrophages, α7 nicotinic acetylcholine receptor (α7nAChR), Janus kinase 2 (JAK2) and signal transducer and activator of transcription 3 (STAT3) were determined by flow cytometry, Western Blot, qPCR and immunofluorescence. The effects of α7nAChR antagonists (methyllycaconitine and α-bungarotoxin) and JAK2/STAT3 inhibitors (AG490 and WP1066) were also administered in a subset of mice prior to EA. In the parasympathetic pathways, intestinal motility and inflammation were determined after cervical vagotomy and sub-diaphragmatic vagotomy. The expression of gamma absorptiometry aminobutyric acid (GABAA) receptor in dorsal motor nucleus of vagal (DMV) cholinergic neurons was assessed by immunofluorescence and the response to DMV microinjection of bicuculine (antagonist of GABAA receptor) or muscimol (agonist of GABAA receptor) were assessed. Results: EA suppressed intestinal inflammation and promoted gastrointestinal motility. Mechanistically, EA activated the α7nAChR-mediated JAK2/STAT3 signaling pathway in macrophages which reduced the production of inflammatory cytokines. Furthermore, we also demonstrated that hindlimb region stimulation drove vagal efferent output by inhibiting the expression of GABAA receptor in DMV to ameliorate inflammation. Conclusions: The present study revealed that EA of hindlimb regions inhibited the expression of GABAA receptor in DMV neurons, whose excited vagal nerve, in turn suppressed IM-induced inflammation via activation of α7nAChR-mediated JAK2/STAT3 signaling pathway.

13.
Bioprocess Biosyst Eng ; 44(7): 1491-1499, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33710454

RESUMO

The red alga Porphyridium purpureum has been known to produce polyunsaturated fatty acids, especially arachidonic acid (ARA), under stressful conditions. However, there is no consistent conclusion about the response of ARA in this alga to nitrogen (N) stress. Also, no research has been done to clearly elucidate the underlying molecular mechanisms of N stress. In this work, P. purpureum CoE1 was cultivated under nitrogen limitation conditions and the putative Δ5-desaturase related gene FADSD5 was isolated. The results showed that the fatty acids in P. purpureum CoE1 were significantly higher in the N limited cultures (54.3 mg g-1) than in the N-replete cultures (45.3 mg g-1) at the 18th day (t-test, p < 0.001), which was attributed to the upregulated abundance of the putative Δ5-desaturase related protein, Δ5-Des. The study also indicated that the expression of the putative Δ5-desaturase related gene, FADSD5, increased with cell growth, demonstrating considerable potentials for ARA biosynthesis in P. purpureum CoE1. These results might guide the direction in illuminating the biosynthetic pathway of fatty acids with molecular evidence and enable genetic modifications of P. purpureum CoE1 for enhancing the ARA accumulation.

14.
Ann Neurol ; 89(5): 872-883, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33704826

RESUMO

OBJECTIVE: The aim was to determine the prevalence and risk factors for electrographic seizures and other electroencephalographic (EEG) patterns in patients with Coronavirus disease 2019 (COVID-19) undergoing clinically indicated continuous electroencephalogram (cEEG) monitoring and to assess whether EEG findings are associated with outcomes. METHODS: We identified 197 patients with COVID-19 referred for cEEG at 9 participating centers. Medical records and EEG reports were reviewed retrospectively to determine the incidence of and clinical risk factors for seizures and other epileptiform patterns. Multivariate Cox proportional hazards analysis assessed the relationship between EEG patterns and clinical outcomes. RESULTS: Electrographic seizures were detected in 19 (9.6%) patients, including nonconvulsive status epilepticus (NCSE) in 11 (5.6%). Epileptiform abnormalities (either ictal or interictal) were present in 96 (48.7%). Preceding clinical seizures during hospitalization were associated with both electrographic seizures (36.4% in those with vs 8.1% in those without prior clinical seizures, odds ratio [OR] 6.51, p = 0.01) and NCSE (27.3% vs 4.3%, OR 8.34, p = 0.01). A pre-existing intracranial lesion on neuroimaging was associated with NCSE (14.3% vs 3.7%; OR 4.33, p = 0.02). In multivariate analysis of outcomes, electrographic seizures were an independent predictor of in-hospital mortality (hazard ratio [HR] 4.07 [1.44-11.51], p < 0.01). In competing risks analysis, hospital length of stay increased in the presence of NCSE (30 day proportion discharged with vs without NCSE: HR 0.21 [0.03-0.33] vs 0.43 [0.36-0.49]). INTERPRETATION: This multicenter retrospective cohort study demonstrates that seizures and other epileptiform abnormalities are common in patients with COVID-19 undergoing clinically indicated cEEG and are associated with adverse clinical outcomes. ANN NEUROL 2021;89:872-883.


Assuntos
/epidemiologia , Eletroencefalografia/tendências , Convulsões/epidemiologia , Convulsões/fisiopatologia , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco , Convulsões/diagnóstico , Resultado do Tratamento
15.
J Dent ; 108: 103633, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33716101

RESUMO

OBJECTIVE: To investigate the remineralizing effect of a strontium-doped bioactive glass (HX-BGC) and fluoride on demineralized enamel and dentine. MATERIALS: Sixty demineralized human tooth specimens were allocated to four groups. Group 1 received 5% HX-BGC, Group 2 received 5% HX-BGC and 1450 ppm fluoride, Group 3 received 1450 ppm fluoride, and Group 4 received deionized water as negative control. The specimens were subjected to pH cycling for 14 days. The surface morphology, lesion depths, crystal characteristics and collagen matrix degradation of the specimens were assessed by scanning electron microscopy (SEM), micro-computed tomography (mico-CT), X-ray diffraction (XRD), and spectrophotometry with a hydroxyproline (HYP) assay, respectively. RESULTS: SEM images showed the enamel surface was smooth with regularly arranged enamel rods in Groups 1-3. Granular grains were observed in both inter-tubular and intra-tubular dentine in Groups 1-3. The mean lesion depths in enamel were 80.8 µm, 50.6 µm, 72.7 µm and 130.7 µm in Groups 1-4, respectively (p < 0.001), and those in dentine were 152.6 µm, 140.9 µm, 165.4 µm and 214.1 µm, respectively (p < 0.001). The differences in mean mineral loss in enamel and in dentine between the four study groups follow the same pattern as that of the differences in lesion depth. XRD illustrated apatite formation in each group. There were no significant differences in the HYP concentrations among the four groups (p = 0.261). CONCLUSION: Combined use of HX-BGC and fluoride can reduce mineral loss and promote remineralization of demineralized enamel and dentine through the precipitation of newly formed apatite. CLINICAL SIGNIFICANCE: Adjunctive use of HX-BGC may enhance the remineralization effect of fluoride in the management of early dental caries lesions.


Assuntos
Cárie Dentária , Fluoretos , Cariostáticos/farmacologia , Cárie Dentária/tratamento farmacológico , Esmalte Dentário , Dentina , Fluoretos/farmacologia , Humanos , Estrôncio/farmacologia , Remineralização Dentária , Microtomografia por Raio-X
16.
J Orthop Surg Res ; 16(1): 229, 2021 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-33781290

RESUMO

OBJECTIVE: The aim of this meta-analysis was to systematically evaluate the clinical efficacy and safety of short-course chemotherapy (≤ 6 months) compared with the standard therapy (9-18 months) for patients with spinal tuberculosis (TB) undergoing surgery in Chinese population. METHODS: In this meta-analysis, we searched electronic databases in the Cochrane Library, PubMed, Embase, China National Knowledge Infrastructure (CNKI), Chinese Science and Technology Periodical Database (VIP), and Wanfang data to determine the equivalence of short-course therapy (group A) and standard therapy (group B) for the drug therapy of TB in Chinese population up to December 24, 2019. Weighted mean difference (WMD), odds risk (OR), and their 95% confidence interval (CI) were calculated. All analyses of relevant outcome indicators were managed by using the Review Manager (RevMan) 5.2 software. RESULTS: This meta-analysis included six trials published involving 851 patients (group A, 397; group B, 454) with spinal TB. Results showed there were no significant differences between group A and group B in clinical cure rate (OR = 0.61; 95% CI 0.19-2.00, p > 0.05), change of erythrocyte sedimentation rate (ESR) (WMD = - 0.75; 95% CI - 3.33 to 1.83; p > 0.05) and bone graft fusion rate (OR = 2.32; 95% CI 0.36-14.81, p > 0.05). Meanwhile, there were fewer side effects (OR = 0.37; 95% CI 0.24-0.58, p < 0.05) in group A compared with group B. CONCLUSIONS: The results of this meta-analysis showed that for patients with spinal TB undergoing surgery in Chinese population, short-course chemotherapy could be equivalent to the standard chemotherapy in terms of efficacy and have less side effects than the latter.

17.
Health Qual Life Outcomes ; 19(1): 60, 2021 Feb 23.
Artigo em Inglês | MEDLINE | ID: mdl-33622357

RESUMO

OBJECTIVE: To sinicize the Supportive Supervisory Scale (SSS) and analyze the psychometric properties of the Chinese version of SSS (SSS-C). METHODS: The SSS (the original English version) was firstly sinicized and adjusted, then its psychometric properties were examined in 300 health care aides from four long-term care (LTC) facilities. SPSS 22.0 was used to process the data and calculate the reliability and validity. RESULTS: The 15-item SSS-C had satisfactory internal consistency (Cronbach's α coefficient = 0.852), split half reliability (Spearman-Brown coefficient = 0.834) and test-retest reliability (Pearson correlation coefficient = 0.784), and three factors were extracted. If the four items with their communality < 0.4 were deleted, the remaining 11 items could explain 55.654% of the total variance. The discriminant validity of the SSS-C varied significantly between sites. CONCLUSIONS: The Chinese version of SSS can be used to effectively measure the supervisory support of the nurses within the LTC settings.


Assuntos
Enfermeiras Administradoras/psicologia , Psicometria/métodos , Inquéritos e Questionários/normas , Adulto , Grupo com Ancestrais do Continente Asiático , Atitude do Pessoal de Saúde , China , Atenção à Saúde , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Psicometria/instrumentação , Qualidade de Vida , Reprodutibilidade dos Testes
18.
ChemSusChem ; 14(6): 1496-1506, 2021 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-33576193

RESUMO

Biomass is the only renewable organic carbon resource in nature, and the transformation of abundant biomass into various chemicals has received immense spotlight. As a novel generation of designer solvents, deep eutectic solvents (DESs) have been widely used in biorefinery due to their excellent properties including low cost, easy preparation, and biodegradability. Although there have been some reports summarizing the performance of DESs for the transformation of biomass into various chemicals, few Reviews illuminate the relationship between the functional structure of DESs and catalytic conversion of biomass. Hence, this Minireview comprehensively summarizes the effects of the types of functional groups in DESs on catalytic conversion of biomass into furanic derivatives, such as carboxylic acid-based hydrogen-bond donors (HBDs), carbohydrate-based HBDs, polyalcohol-based HBDs, amine/amide-based HBDs, spatial structure of HBDs, and various hydrogen-bond acceptors (HBAs). It also further summarizes the effects of adding different additives into the DESs on the synthesis of high value-added chemicals, including water, liquid inorganic acids, Lewis acids, heteropoly acids, and typical solid acids. Moreover, current challenges and prospects for the application of DESs in biomass conversion are provided.

19.
J Thorac Imaging ; 2021 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-33560763

RESUMO

PURPOSE: The aim of the study was to evaluate the role of cardiac magnetic resonance (CMR) mapping and strain analysis in the identification of cardiac involvement in idiopathic inflammatory myopathy (IIM) patients with preserved left ventricular ejection fraction. MATERIALS AND METHODS: In all, 38 IIM patients who underwent CMR examination at our institution were retrospectively included. Twenty-three age-matched healthy individuals served as controls. Mapping parameters including native T1, extracellular volume (ECV), and T2 mapping and strain parameters including global radial strain, global circumferential strain, and global longitudinal strain were measured semiautomatically using a dedicated processing software. All the mapping and strain values were compared between patients and controls. RESULTS: Late gadolinium enhancement was only present in IIM patients (n=17, 44.7%). IIM patients showed higher native T1 (1346 vs. 1269 ms, P<0.001), ECV (31.1% vs. 27.4%, P<0.01), and higher T2 (44.4 vs. 39.2 ms, P<0.001) values compared with controls. The global radial strain (36.7% vs. 46.9%, P<0.001), global circumferential strain (-21.2% vs. -24.1%, P<0.01), and global longitudinal strain (-13.6% vs. -15.6%, P<0.05) values were significantly reduced compared with controls. CONCLUSIONS: Native T1, ECV, T2 values, and global strain values may hold promise for the detection of subclinical myocardial involvement in IIM patients with preserved left ventricular ejection fraction.

20.
Microbiome ; 9(1): 12, 2021 01 12.
Artigo em Inglês | MEDLINE | ID: mdl-33436102

RESUMO

BACKGROUND: Proteolysis regulation allows gut microbes to respond rapidly to dynamic intestinal environments by fast degradation of misfolded proteins and activation of regulatory proteins. However, alterations of gut microbial proteolytic signatures under complex disease status such as inflammatory bowel disease (IBD, including Crohn's disease (CD) and ulcerative colitis (UC)), have not been investigated. Metaproteomics holds the potential to investigate gut microbial proteolysis because semi-tryptic peptides mainly derive from endogenous proteolysis. RESULTS: We have developed a semi-tryptic peptide centric metaproteomic mining approach to obtain a snapshot of human gut microbial proteolysis signatures. This approach employed a comprehensive meta-database, two-step multiengine database search, and datasets with high-resolution fragmentation spectra to increase the confidence of semi-tryptic peptide identification. The approach was validated by discovering altered proteolysis signatures of Escherichia coli heat shock response. Utilizing two published large-scale metaproteomics datasets containing 623 metaproteomes from 447 fecal and 176 mucosal luminal interface (MLI) samples from IBD patients and healthy individuals, we obtain potential signatures of altered gut microbial proteolysis at taxonomic, functional, and cleavage site motif levels. The functional alterations mainly involved microbial carbohydrate transport and metabolism, oxidative stress, cell motility, protein synthesis, and maturation. Altered microbial proteolysis signatures of CD and UC mainly occurred in terminal ileum and descending colon, respectively. Microbial proteolysis patterns exhibited low correlations with ß-diversity and moderate correlations with microbial protease and chaperones levels, respectively. Human protease inhibitors and immunoglobulins were mainly negatively associated with microbial proteolysis patterns, probably because of the inhibitory effects of these host factors on gut microbial proteolysis events. CONCLUSIONS: This semi-tryptic peptide centric mining strategy offers a label-free approach to discover signatures of in vivo gut microbial proteolysis events if experimental conditions are well controlled. It can also capture in vitro proteolysis signatures to facilitate the evaluation and optimization of experimental conditions. Our findings highlight the complex and diverse proteolytic events of gut microbiome, providing a unique layer of information beyond taxonomic and proteomic abundance. Video abstract.


Assuntos
Microbioma Gastrointestinal , Fragmentos de Peptídeos/análise , Fragmentos de Peptídeos/metabolismo , Proteólise , Proteômica , Tripsina/metabolismo , Adolescente , Adulto , Criança , Escherichia coli/metabolismo , Humanos , Pessoa de Meia-Idade , Fragmentos de Peptídeos/química , Adulto Jovem
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