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1.
Andrologia ; 53(9): e14122, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34319588

RESUMO

Radical prostatectomy, radiotherapy and active surveillance are three widely used treatment options for patients with low-risk prostate cancer, but the relative effects are controversial. We searched PubMed, Embase and Web of Science until June 2020, focusing on the studies comparing the effect of radical prostatectomy, radiotherapy and active surveillance in patients with low-risk prostate cancer. Through the random-effects model, dichotomous data were extracted and summarised by odds ratio with a 95% confidence interval. Twenty-two studies containing 185,363 participants were pooled for the comprehensive comparison. The Bayesian mixed network estimate demonstrated the cancer-specific mortality of radical prostatectomy was significantly lower than active surveillance (OR, 0.46; 95% CI 0.34-0.64) and external beam radiation therapy (OR, 0.66; 95% CI 0.46-0.96), but not brachytherapy (OR, 0.63; 95% CI 0.41-1.03). The brachytherapy demonstrated the best treatment ranking probability results in terms of all-cause mortality, while no significant difference was observed when compared with other three treatment modalities. Brachytherapy and radical prostatectomy were associated with a similar risk of cancer-specific mortality, and both of them were significantly superior to active surveillance and external beam radiation therapy; nevertheless, there was no significant difference among the aforementioned treatment methods in all-cause mortality.


Assuntos
Braquiterapia , Neoplasias da Próstata , Teorema de Bayes , Humanos , Masculino , Metanálise em Rede , Prostatectomia , Neoplasias da Próstata/radioterapia , Neoplasias da Próstata/cirurgia
2.
J Ovarian Res ; 14(1): 25, 2021 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-33536040

RESUMO

BACKGROUND: To describe the diagnostic criteria used and their application accuracy in the practice of polycystic ovary syndrome (PCOS) caring among obstetricians and gynaecologists across China. METHODS: This was an Online cross-sectional survey of Obstetricians and gynecologists involved in PCOS caring conducted via the largest continuing education platform of obstetrics and gynecology across China from September 2019 to November 2019. RESULTS: A total of 2,328 respondents were eligible for the final analysis. Of these, 94.5 % were general obstetricians and gynaecologists (Ge-ObGyn), and 5.5 % were reproductive endocrinologists (Re-ObGyn). Overall, the most frequently used criteria were the Androgen Excess and Polycystic Ovary Syndrome Society (AE-PCOS) criteria (48.2 %), followed by the Rotterdam criteria (35.7 %) and NIH criteria (12.1 %). Of the respondents, 31.3 % used their diagnostic criteria in their clinical practice. More respondents who chose the Rotterdam criteria could accurately apply the diagnostic criteria than those who chose the AE-PCOS criteria (41.2 % vs. 32.1 %, P < 0.001). Compared with Ge-ObGyn, Re-ObGyn were less likely to use the AE-PCOS criteria (adjusted odds ratio, 0.513; 95 % CI, 0.328-0.802; P < 0.05) and 1.492 times more likely to accurately use their criteria (95 % CI, 1.014-2.196; P < 0.05). CONCLUSIONS: Less than one-third of obstetricians and gynaecologists across China could accurately use the diagnostic criteria they choose to diagnose PCOS. There is an urgent need to train obstetricians and gynaecologists on PCOS diagnosis in an effort to improve the medical care quality of patients with PCOS.


Assuntos
Competência Clínica , Ginecologia , Obstetrícia , Síndrome do Ovário Policístico/diagnóstico , Padrões de Prática Médica , Adulto , China , Endocrinologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Razão de Chances , Inquéritos e Questionários , Adulto Jovem
3.
Cell Biosci ; 10: 83, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32626571

RESUMO

Background: Hepatocellular carcinoma (HCC) is one of the most malignant tumor types and has a high incidence and mortality. Many miRNAs play important roles in the development of HCC. Identification of these miRNAs and their targets is increasingly urgent for a better understandingof miRNA function in both physiological and pathological contexts. Many studies have shown that the expression of let-7 is often downregulated in the process of tumorigenesis, suggesting that let-7 may participate in this process as an oncogene. Methods: Immunochemistry staining was used to observe the expression of let-7b in HCC tissues. A CCK-8 assay was employed to detect the role of let-7b in the proliferation of HCC cells. The cell cycle of HCC cells was examined by flow cytometry. BALB/c nu/nu mice were used to detect the tumorigenesis potential of HCC cells; western blot and real-time PCR were employed to observe the expression of p21 in HCC cells. Results: In our previous studies investigating HCC tissue samples obtained from the national tissue samples bank of liver cancer in Eastern Hepatobiliary Surgery Hospital, we found one abnormal expression of miRNA (let-7b), which was significantly downregulated in HCC tissue. In the current work, we studied the relationship between let-7b and HCC to potentially provide invaluable information for developing novel therapeutic strategies for treating HCC. Based on our findings, let-7b expression was absent in HCC tumors, and its lower expression was associated with poor prognosis of HCC. In further experiments, we found that let-7b inhibited HCC cell proliferation through upregulation of p21. Conclusion: The results of our study suggested that let-7b might inhibit the proliferation of HCC cells by upregulating p21.

4.
Indian J Crit Care Med ; 24(4): 283-284, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32565641

RESUMO

How to cite this article: Xiao-Bo H, Poonyathawon S, Semedi BP, Xiao-Yi Z, Wei F, Da-Wei W, et al. International-focused Online Forum: A Good Way to Jointly Manage the COVID-19 Pandemic for Global Critical Care Community. Indian J Crit Care Med 2020;24(4):283-284.

5.
Preprint em Inglês | medRxiv | ID: ppmedrxiv-20093732

RESUMO

Introductory paragraphThe pandemic of coronavirus Disease 2019 (COVID-19) caused enormous loss of life globally. 1-3 Case identification is critical. The reference method is using real-time reverse transcription PCR (rRT-PCR) assays, with limitations that may curb its prompt large-scale application. COVID-19 manifests with chest computed tomography (CT) abnormalities, some even before the onset of symptoms. We tested the hypothesis that application of deep learning (DL) to the 3D CT images could help identify COVID-19 infections. Using the data from 920 COVID-19 and 1,073 non-COVID-19 pneumonia patients, we developed a modified DenseNet-264 model, COVIDNet, to classify CT images to either class. When tested on an independent set of 233 COVID-19 and 289 non-COVID-19 patients. COVIDNet achieved an accuracy rate of 94.3% and an area under the curve (AUC) of 0.98. Application of DL to CT images may improve both the efficiency and capacity of case detection and long-term surveillance.

6.
J Drugs Dermatol ; 18(6): 581, 2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-31251552

RESUMO

The skin is one of the largest immunologic organs in the body and a continuous target for allergic and immunologic responses. Impairment of the skin barrier increases the likelihood of external antigens and pathogens entering and creating inflammation, which can potentially lead to skin infections, allergies, and chronic inflammatory diseases such as atopic and contact dermatitis. Functionally, the skin barrier can be divided into four different levels. From outermost to innermost, these highly interdependent levels are the microbiome, chemical, physical, and immune levels. The objective of this review is to provide an update on current knowledge about the relationship between skin barrier function and how dysfunction at each level of the skin barrier can lead to allergic sensitization, contact dermatitis, and the atopic march, and examine how to best repair and maintain this barrier through the use of moisturizers. J Drugs Dermatol. 2019;18(6):581-586.


Assuntos
Dermatite Alérgica de Contato/prevenção & controle , Dermatite Atópica/prevenção & controle , Emolientes/administração & dosagem , Dermatopatias Infecciosas/prevenção & controle , Pele/metabolismo , Dermatite Alérgica de Contato/imunologia , Dermatite Alérgica de Contato/microbiologia , Dermatite Atópica/imunologia , Dermatite Atópica/microbiologia , Disbiose/imunologia , Disbiose/microbiologia , Disbiose/prevenção & controle , Humanos , Microbiota/imunologia , Permeabilidade/efeitos dos fármacos , Pele/efeitos dos fármacos , Pele/microbiologia , Creme para a Pele/administração & dosagem , Dermatopatias Infecciosas/imunologia , Dermatopatias Infecciosas/microbiologia
7.
Biochem Pharmacol ; 152: 153-164, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29574068

RESUMO

Adriamycin (ADM) is a commonly used drug in clinical breast cancer treatment. However, some breast cancer types or breast cancers subjected to repeated ADM exposure develop strong resistance to ADM thus limiting its clinical efficacy. In this study, we found for the first time that toosendanin (TSN), a triterpenoid extracted from the traditional Chinese medicine Melia toosendan Sieb et Zucc, could successfully reverse adriamycin resistance in human breast cancer cells. Immunofluorescence and HPLC analysis demonstrated that TSN promoted adriamycin accumulation in breast cancer cells, especially in the nucleus. Furthermore, TSN could significantly reduce ABCB1 expression. We then found that TSN was capable of suppressing adriamycin-induced Akt phosphorylation, probably due to downregulation of the PI3K catalytic subunits P110α and P110ß, and inhibition of DNA-PKcs. Importantly, the inhibitory effect of TSN on PI3K P110α and P110ß expression was specifically observed in breast cancer cells but not in normal human cells. Moreover, TSN significantly potentiated the anti-cancer effect of ADM in the 4T1 breast cancer model and its inhibition rate was nearly 90%. Thus, TSN could be used as a novel PI3K inhibitor to reverse breast cancer resistance. The combination of ADM and TSN may represent a useful strategy for human breast cancer therapy.


Assuntos
Neoplasias da Mama/tratamento farmacológico , Doxorrubicina/farmacologia , Resistencia a Medicamentos Antineoplásicos , Medicamentos de Ervas Chinesas/farmacologia , Animais , Antineoplásicos/administração & dosagem , Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Linhagem Celular , Doxorrubicina/administração & dosagem , Medicamentos de Ervas Chinesas/administração & dosagem , Medicamentos de Ervas Chinesas/química , Feminino , Humanos , Camundongos Endogâmicos BALB C , Estrutura Molecular , Neoplasias Experimentais/tratamento farmacológico , Fosfatidilinositol 3-Quinases , Fitoterapia
8.
Annu Int Conf IEEE Eng Med Biol Soc ; 2017: 3348-3351, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29060614

RESUMO

The relation between music and emotions has been investigated for decades. Most of the studies focused on short clips and were designed with specific tasks. This paper investigated the emotional states from electroencephalogram (EEG) activities during music appreciation. An emotion evoked experiment paradigm was designed during music appreciation. The EEG signals were recorded in 15 healthy adults during the entire process of music listening. The band power change (BPC) and higher order crossing (HOC) features were extracted from the EEG signals. A correlation-based feature analysis approach was proposed to find the most relevant features in time, frequency and channel space domains. From the results, this method achieved the average accuracy of 67.2% for the classification of high and low valence in the combination of BPC and HOC features. A deeper understanding of the brain emotional patterns could be helpful in building an intelligent and friendly affective application.


Assuntos
Emoções , Percepção Auditiva , Encéfalo , Eletroencefalografia , Música
9.
IEEE Trans Image Process ; 26(12): 5980-5993, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28866493

RESUMO

The tremendous growth in mobile devices has resulted in huge generation and usage of digital images. Image quality assessment is thus an important issue for mobile media applications. In this paper, we focus on the quality evaluation of images generated by content-aware image retargeting, in which the reference and the distorted images are of different sizes. Through retargeting, many types of deformation inconsistency lead to shape distortion, deformation artifacts, and content information loss, worsening its perceptual quality. The deformation inconsistency occurs on different levels of the retargeted images. Limited by the accuracy of the alignment between the original and retargeted images, previous methods only focus on pixel-level and patch-level fidelity analyses and fail to detect deformation inconsistency. In this paper, we improve the alignment algorithm and propose a three-level representation of the retargeting process. Based on the analysis of this three-level representation, both fidelity measures and inconsistency detection are combined to determine the final retargeting quality. The proposed algorithm is validated on the public data sets RetargetMe and CUHK. Experimental results demonstrate that inconsistency detection contributes to accurately assessing the image retargeting perceptual quality. This inspires us to investigate more about deformation inconsistency to formulate the objective quality of image retargeting.

10.
Artigo em Inglês | MEDLINE | ID: mdl-28649028

RESUMO

OBJECTIVES: The quality safety supervision and test of agricultural products urgently need a very excellent analytical method with simultaneously detecting many components in order to assess, prevent and control pesticide residues. METHODS: In this research, three fruits and three vegetables produced in Shanghai were selected as the materials, 54 pesticide residues were detected with the ultra-high performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) method on the basis of optimized QuEChERS method according to different materials properties. RESULTS: The results showed that: all samples were directly extracted by acetonitrile containing 1% (v/v) acetic acid; complex matrix samples were purified by a mixed sorbent of 300mg MgSO4+100mg PSA+100mg C18+0.01g Carb, general matrix samples didn't add Carb, simple matrix samples such as watermelon directly filmed; Chromatographic column was ZORBAX Eclipse Plus-C18 column (3.0mm×150mm, 3.5µm) at 40°C, the methanol-water of mobile phase at a flow rate of 0.45mL/min by a gradient elution contained 0.1% formic acid and 5mmol/L ammonium acetate and the injection volume was 1µL. With switching electrospray ion source polarity, [M-H]- and high sensitive [M+Na]+were respectively the precursor ions of eight pesticides and avermectin, [M+H]+ was those of the else 45 pesticides. The detection parameters of multi-reactions monitoring (MRM) with simultaneously positive and negative ions (electron multiplier voltage was 200V) scanning were set as follows: the 310.3kPa of nebulizer pressure, the 300°C of drying gas temperature, the 7L/min of drying gas flow, the 3000V and 3500V of respectively capillary positive and negative voltage. With the optimized method, the calibration curves of 54 pesticides were better linear in 15-500µg/kg (r ≥0.988), the average adding standard recovery rates of 54 pesticides were 73.2%-134.3% except pymetrozine and cyromazine with the relative standard deviations (RSD) of 1.0%-13.8%; the limit of detection (LOD) under three times signal-to-noise ratio (S/N) and limit of quantitative (LOQ) under 10 times S/N were respectively confirmed 0.003-2.000µg/kg and 0.01-6.67µg/kg. CONCLUSIONS: The results demonstrated that the optimized "QuEChERS-UPLC-MS/MS method" was a simple, rapid, sensitive, accurate, efficient, economical and safe method that simultaneously detected multiple pesticide residues through one time sample treatment; It had some advantages such as more pesticides per detection, simple and convenient pretreatment and less solvent dosage to be suitable for the quick high-throughput quantitative screening and confirmation of pesticide residues in fruits and vegetables.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Frutas/química , Resíduos de Praguicidas/análise , Espectrometria de Massas em Tandem/métodos , Verduras/química , Limite de Detecção , Modelos Lineares , Reprodutibilidade dos Testes
11.
Saudi J Biol Sci ; 24(3): 548-555, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28386179

RESUMO

Estimating the functional interactions and connections between brain regions to corresponding process in cognitive, behavioral and psychiatric domains is a central pursuit for understanding the human connectome. Few studies have examined the effects of dynamic evolution on cognitive processing and brain activation using brain network model in scalp electroencephalography (EEG) data. Aim of this study was to investigate the brain functional connectivity and construct dynamic programing model from EEG data and to evaluate a possible correlation between topological characteristics of the brain connectivity and cognitive evolution processing. Here, functional connectivity between brain regions is defined as the statistical dependence between EEG signals in different brain areas and is typically determined by calculating the relationship between regional time series using wavelet coherence. We present an accelerated dynamic programing algorithm to construct dynamic cognitive model that we found that spatially distributed regions coherence connection difference, the topologic characteristics with which they can transfer information, producing temporary network states. Our findings suggest that brain dynamics give rise to variations in complex network properties over time after variation audio stimulation, dynamic programing model gives the dynamic evolution processing at different time and frequency. In this paper, by applying a new construct approach to understand whole brain network dynamics, firstly, brain network is constructed by wavelet coherence, secondly, different time active brain regions are selected by network topological characteristics and minimum spanning tree. Finally, dynamic evolution model is constructed to understand cognitive process by dynamic programing algorithm, this model is applied to the auditory experiment, results showed that, quantitatively, more correlation was observed after variation audio stimulation, the EEG function connection dynamic evolution model on cognitive processing is feasible with wavelet coherence EEG recording.

12.
Otol Neurotol ; 36(9): 1543-6, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26208130

RESUMO

OBJECTIVE: To report a rare case of recurrent temporal bone carcinoma encasing the carotid artery. PATIENT: A 54-year-old man underwent mastoidectomy combined with adjuvant chemoradiotherapy of squamous cell carcinoma of the temporal bone. He developed metastatic disease and received parotidectomy and lymph node dissection. Three months later, the magnetic resonance imaging scan showed that the tumor had relapsed and invaded the carotid artery. INTERVENTION: The patient completed TPF-C induction chemotherapy (docetaxel, cisplatin, 5-fluorouracil, cetuximab), followed by concurrent chemotherapy with reirradiation. RESULTS: Magnetic resonance imaging revealed a complete response of tumor 1 month after treatment. The patient had no detectable recurrence after 53 months of follow-up. CONCLUSION: Concurrent chemotherapy with reirradiation is a potential curative treatment option for patients who have unresectable recurrent squamous cell carcinoma of the temporal bone.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Carcinoma de Células Escamosas/terapia , Neoplasias de Cabeça e Pescoço/terapia , Recidiva Local de Neoplasia/terapia , Neoplasias Cranianas/terapia , Osso Temporal/patologia , Carcinoma de Células Escamosas/patologia , Cetuximab/administração & dosagem , Quimiorradioterapia/métodos , Cisplatino/administração & dosagem , Docetaxel , Fluoruracila/administração & dosagem , Neoplasias de Cabeça e Pescoço/patologia , Humanos , Quimioterapia de Indução/métodos , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia/patologia , Reirradiação/métodos , Neoplasias Cranianas/patologia , Carcinoma de Células Escamosas de Cabeça e Pescoço , Taxoides/administração & dosagem
13.
Mol Biol Cell ; 26(6): 1044-57, 2015 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-25589675

RESUMO

General control nonderepressible kinase 2 (GCN2) is a promising target for cancer therapy. However, the role of GCN2 in cancer cell survival or death is elusive; further, small molecules targeting GCN2 signaling are not available. By using a GCN2 level-based drug screening assay, we found that GCN2 protein level critically determined the sensitivity of the cancer cells toward Na(+),K(+)-ATPase ligand-induced apoptosis both in vitro and in vivo, and this effect was largely dependent on C/EBP homologous protein (CHOP) induction. Further analysis revealed that GCN2 is a short-lived protein. In A549 lung carcinoma cells, cellular ß-arrestin1/2 associated with GCN2 and maintained the GCN2 protein level at a low level by recruiting the E3 ligase NEDD4L and facilitating consequent proteasomal degradation. However, Na(+),K(+)-ATPase ligand treatment triggered the phosphorylation of GCN2 at threonine 899, which increased the GCN2 protein level by disrupting the formation of GCN2-ß-arrestin-NEDD4L ternary complex. The enhanced GCN2 level, in turn, aggravated Na(+),K(+)-ATPase ligand-induced cancer cell apoptosis. Our findings reveal that GCN2 can exert its proapoptotic function in cancer cell death by posttranslational mechanisms. Moreover, Na(+),K(+)-ATPase ligands emerge as the first identified small-molecule drugs that can trigger cancer cell death by modulating GCN2 signaling.


Assuntos
Apoptose , Proteínas Serina-Treonina Quinases/fisiologia , Ubiquitinação , Arrestinas/metabolismo , Complexos Endossomais de Distribuição Requeridos para Transporte/metabolismo , Expressão Gênica , Células HCT116 , Células HT29 , Humanos , Ubiquitina-Proteína Ligases Nedd4 , Fosforilação , Proteólise , Fator de Transcrição CHOP/metabolismo , Ubiquitina-Proteína Ligases/metabolismo , beta-Arrestinas
14.
EMBO Mol Med ; 7(2): 140-57, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25535255

RESUMO

Immunoparalysis is an important pathological mechanism in sepsis. However, an effective small molecule therapy is lacking. Here, we show that ouabain, a Na(+),K(+)-ATPase ligand, can reverse immunoparalysis in vitro, in vivo, and in clinical samples. Notably, the effect of ouabain was critically dependent on TNF-α expression. However, ouabain had opposing effects on the stability of TNF-α mRNA: Ouabain triggered miR-181 transcription, which promoted TNF-α mRNA degradation and induced immunoparalysis, and ouabain triggered the nuclear export of human antigen R (HuR), which stabilized TNF-α mRNA and suppressed immuno-paralysis. Interestingly, because the miR-181 binding site is located within the HuR binding site in the 3'-untranslated region of TNF-α, in ouabain-treated cells, HuR competed with miR-181 for binding to TNF-α mRNA and recruited TNF-α mRNA to stress granules, thereby stabilizing TNF-α mRNA and reversing immunoparalysis. Ouabain also induced GM-CSF and interferon-γ expression in a HuR-dependent manner. Hence, the fine-tuning of TNF-α mRNA stability by HuR and miR181 plays a crucial role in immunoparalysis, and Na(+),K(+)-ATPase ligands are promising agents for immunoparalysis therapy.


Assuntos
Proteínas ELAV/metabolismo , MicroRNAs/metabolismo , Sepse/genética , Sepse/imunologia , Fator de Necrose Tumoral alfa/genética , Regiões 3' não Traduzidas , Adulto , Idoso , Animais , Proteínas ELAV/genética , Feminino , Humanos , Masculino , Camundongos Endogâmicos C57BL , MicroRNAs/genética , Pessoa de Meia-Idade , Ouabaína/administração & dosagem , Regiões Promotoras Genéticas , Estabilidade de RNA , Sepse/tratamento farmacológico , Sepse/metabolismo , Fator de Necrose Tumoral alfa/química , Fator de Necrose Tumoral alfa/metabolismo
15.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 32(6): 542-6, 2014 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-25665416

RESUMO

OBJECTIVE: To study the effect of excessive fluoride on calcium overload and apoptosis in cultured rat ameloblasts in vitro. METHODS: Logarithmic-phase ameloblasts (HAT-7) were treated with 0, 0.4, 0.8, 1.6, 3.2, and 6.4 mmol · L(-1) sodium fluoride (NaF) solution. Cell activities were detected by using a Cell Counting Kit 8 (CCK-8) assay after 48 h of treatment. The effect of fluoride on cell apoptosis was analyzed by using flow cytometry. Excessive fluoride-induced calcium concentration and calreticulin expression changes in ameloblasts were detected by using laser scanning confocal microscopy, Western blot analysis, and real-time quantitative polymerase chain reaction. RESULTS: NaF inhibited ameloblast activity at 1.6, 3.2, and 6.4 mmol · L(-1) (dose-dependent) after 48 h of induction. The Ca2+ fluorescence intensity of HAT-7 cells incubated with 1.6 and 3.2 mmol · L(-1) NaF was higher than that in the control group. The fluoride-induced early-stage apoptosis of ameloblasts after 48 h of induction and the early-stage apoptosis rate was positively correlated with fluoride concentration. Calreticulin mRNA expression in HAT-7 cells was higher than that in the control group after 48 h of incubation with 0.8, 1.2, and 1.6 mmol · L(-1) NaF. CONCLUSION: Excessive fluoride-induced calcium overload in ameloblasts and further caused endoplasmic reticulum stress-mediated apoptosis.


Assuntos
Ameloblastos , Fluoreto de Cálcio , Animais , Apoptose , Cálcio , Fluoretos , Fosfatos , Ratos , Fluoreto de Sódio
16.
Neurol Sci ; 35(6): 815-21, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24337989

RESUMO

Chemotherapy drugs treatment causes neuropathic pain, hyperalgesia and allodynia are common components of neuropathic pain, so effectively therapeutic strategy is required. In this study, we evaluated the antinociceptive effects of matrine on vincristine-induced neuropathic pain in mice. Vincristine (100 µg/kg i.p.) was administered once per day for 7 days (day 0-6) in mice. Matrine (15, 30, 60 mg/kg, i.p.) was repeated administration in early phase (day 0-6) or late phase (day 7-13). Hyperalgesia and allodynia were evaluated by withdrawal response using von Frey filaments, plantar and cold-plate on 7, 14 and 21 day. Injection of vincristine produced mechanical hyperalgesia and cold allodynia. Matrine was found to produce a protective role in both von Frey filaments and cold-plate test. The analysis of the effect supports the hypothesis that matrine is useful in therapy of vincristine-induced neuropathic pain. In conclusion, this study demonstrates that administration of matrine is associated with antinociceptive effect on mechanical and cold stimuli in a mice model of vincristine-induced neuropathy pain.


Assuntos
Alcaloides/uso terapêutico , Analgésicos/uso terapêutico , Antineoplásicos Fitogênicos/toxicidade , Neuralgia/tratamento farmacológico , Quinolizinas/uso terapêutico , Vincristina/toxicidade , Animais , Modelos Animais de Doenças , Hiperalgesia/tratamento farmacológico , Camundongos , Camundongos Endogâmicos ICR , Neuralgia/induzido quimicamente , Limiar da Dor/efeitos dos fármacos
17.
Planta Med ; 79(16): 1572-5, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-24081685

RESUMO

Arigsugacin I (1), a new α-pyrone meroterpene, along with two known compounds, arigsugacins F (2) and territrem B (3), were isolated from the mangrove endophytic fungus Penicillium sp. sk5GW1L from Kandelia candel. Their structures were identified through mass spectrometry and NMR experiments, and the absolute configuration of compound 1 was further confirmed by low-temperature (100 K) single crystal X-ray diffraction with Cu Kα radiation. The absolute configuration of compound 2 was first reported by using X-ray copper radiation. Compounds 1-3 showed inhibitory activities against acetylcholinesterase with IC50 values of 0.64 ± 0.08 µM, 0.37 ± 0.11 µM, and 7.03 ± 0.20 nM, respectively.


Assuntos
Inibidores da Colinesterase/química , Penicillium/química , Pironas/química , Rhizophoraceae/microbiologia , Terpenos/química , Acetilcolinesterase/química , Inibidores da Colinesterase/isolamento & purificação , Ressonância Magnética Nuclear Biomolecular , Pironas/isolamento & purificação , Terpenos/isolamento & purificação
18.
Planta Med ; 79(11): 916-23, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23807812

RESUMO

In this study, we investigated the neuroprotective effect of oxysophoridine on ischemia and ischemia-like insults. Protection by oxysophoridine was studied at the in vivo level using a model of middle cerebral artery occlusion in mice and at the in vitro level using primary rat hippocampal neuronal cultures exposed to oxygen-glucose deprivation, a model of ischemia-like injury. The behavioral test was performed by using the neurological scores. The infarction volume of brain was assessed in the brain slices stained with 2,3,5-triphenyl tetrazolium chloride. The neuron apoptosis was evaluated by Hoechst 33342 staining. The morphological change in the neurons was examined using a Transmission Electron Microscope (TEM or EM). To evaluate neuron apoptosis, caspase-3, -9, and - 8 activities were measured using assay kits with an ELISA reader. The Western blotting assay was used to evaluate the release of cytochrome c and expression of caspase-3, Bcl-2, and Bax proteins. The quantitative real-time PCR assay was used to evaluate the release of cytochrome c and the expression of caspase-3 mRNA. Oxysophoridine-treated groups (62.5, 125, 250 mg/kg) markedly reduced neurological deficit scores and infarct volumes. Treatment with oxysophoridine (5, 20, 80 µmol/L) significantly attenuated neuronal damage, with evidence of decreased cell apoptosis and decreased cell morphologic impairment. Furthermore, treatment with oxysophoridine could effectively downregulate the expression of cytochrome c and caspase-3 in both mRNA and protein levels, and Bax in the protein level, and induce an increase of Bcl-2 in the protein level. The caspase-3, -9, and -8 activities were also inhibited. These findings suggested that oxysophoridine may be a potential neuroprotective agent for cerebral ischemia injury.


Assuntos
Alcaloides/farmacologia , Apoptose/efeitos dos fármacos , Isquemia Encefálica/tratamento farmacológico , Infarto da Artéria Cerebral Média/tratamento farmacológico , Fármacos Neuroprotetores/farmacologia , Animais , Encéfalo/metabolismo , Caspase 3/genética , Caspase 3/metabolismo , Citocromos c/genética , Citocromos c/metabolismo , Hipocampo/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos ICR , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Organismos Livres de Patógenos Específicos , Proteína X Associada a bcl-2/metabolismo
19.
Pharm Biol ; 51(7): 844-50, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23627473

RESUMO

CONTEXT: Sophora alopecuroides L. (Leguminosae) is a commonly used Chinese herbal drug that possesses antipyretic, anti-inflammatory and analgesic effects. Among various alkaloids isolated from S. alopecuroides, matrine has been identified as the major bioactive component contributing to a variety of pharmacological effects, and studies have also shown that matrine has an analgesic effect. OBJECTIVE: To investigate the antinociceptive effects of matrine on neuropathic pain induced by chronic constriction injury (CCI) in mice. MATERIALS AND METHODS: The von Frey, plantar, cold-plate, locomotor activity and rota-rod test were performed to assess the degree of mechanical, radiant, thermal, spontaneous locomotor activity and motor coordination changes respectively, at different time intervals, i.e., one day before surgery and 7, 8, 10, 12 and 14 days post surgery. Matrine was administered from the 8th day after the surgery for seven days. RESULTS: Our present study shows that matrine at the dose of 30 mg/kg i.p. increased the paw withdrawal threshold (0.88 ± 0.16), paw withdrawal latency (7.01 ± 0.11) and the counts of paw withdrawal (19.7 ± 1.15) from the day 8 for the nerve injured paw compared to the CCI group (0.18 ± 0.04, 4.62 ± 0.18, 44.3 ± 2.99, respectively). Matrine, in a dose-dependent effect, was also found to produce a protective role in both plantar and cold-plate tests. The analysis of the effect supports the hypothesis that matrine is useful in neuropathic pain therapy. DISCUSSION AND CONCLUSION: The results of this study suggest that matrine could be useful in the treatment of different kinds of neuropathic pains as an adjuvant to conventional medicines.


Assuntos
Alcaloides/farmacologia , Analgésicos/farmacologia , Neuralgia/tratamento farmacológico , Quinolizinas/farmacologia , Sophora/química , Alcaloides/administração & dosagem , Alcaloides/isolamento & purificação , Analgésicos/administração & dosagem , Analgésicos/isolamento & purificação , Animais , Constrição Patológica , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Medicamentos de Ervas Chinesas/administração & dosagem , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos ICR , Atividade Motora/efeitos dos fármacos , Quinolizinas/administração & dosagem , Quinolizinas/isolamento & purificação , Fatores de Tempo
20.
J Mol Histol ; 43(5): 535-42, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22562802

RESUMO

This study investigated the protective effects of Lycium barbarum polysaccharide (LBP) on alleviating injury from oxygen-glucose deprivation/reperfusion (OGD/RP) in primary cultured rat hippocampal neurons. Cultured hippocampal neurons were exposed to oxygen-glucose deprivation (OGD) for 2 h followed by a 24 h re-oxygenation. The MTT assay and the lactate dehydrogenase (LDH) release were used to determine the neuron viability. Superoxide dismutase (SOD), Glutathione peroxidase (GSH-PX), malondialdehyde (MDA) were determined by spectrophotometry using commercial kits. Mitochondrial membrane potential (MMP) and the intracellular free calcium concentration ([Ca(2+)](i)) in hippocampal neurons were measured using the confocal laser scanning microscope (CLSM). Treatment with LBP (10-40 mg/l) significantly attenuated neuronal damage and inhibited LDH release in a dose-dependent manner. Furthermore, LBP enhanced activities of SOD and GSH-PX but it decreased their MDA content, inhibited [Ca(2+)](i) elevation and decrease of MMP in ischemia-reperfusion treated hippocampal neurons. These findings suggested that LBP may be a potential neuroprotective agent for cerebral ischemia-reperfusion injury.


Assuntos
Medicamentos de Ervas Chinesas/administração & dosagem , Substâncias Protetoras/administração & dosagem , Traumatismo por Reperfusão/tratamento farmacológico , Animais , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Meios de Cultura/química , Glucose/metabolismo , Hipocampo/citologia , Hipocampo/efeitos dos fármacos , Neurônios/citologia , Neurônios/efeitos dos fármacos , Oxigênio/metabolismo , Ratos , Ratos Sprague-Dawley
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