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1.
J Ethnopharmacol ; 264: 113096, 2021 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-32693116

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Constipation is a functional gastrointestinal disorder and one of the most prevalent conditions encountered in primary care settings. Rhubarb navel dressings have been used for more than 2,000 years in Chinese medicine to treat constipation. However, the effect of topical rhubarb administration has still not been well recognized and this strategy is not yet established as an evidence-based approach. AIM OF THE STUDY: In this study, we performed a prospective multicentric randomized controlled trial to evaluate the efficacy and safety of rhubarb navel plasters for patients with chronic constipation. MATERIALS AND METHODS: A total of 374 patients from six teaching hospitals were prospectively included between 09/2016 and 10/2017 in the study based on Rome III criteria. All participants were randomly assigned (1:1) into verum/placebo group and given either Rheum officinale rhubarb powder or a placebo flour stick on the navel for 6 h/day/8 days. Primary outcome measures were the Cleveland Constipation Score (CCS) for the feces condition and Bristol Stool Scale (BSS) for stool consistency and 24 h defecation frequency. RESULTS: The groups demonstrated no statistical differences in demographic data, clinical diagnoses and concomitant medication at baseline. In patients treated with the verum CCS was 5.61 (day 8, 95% CI 5.15-6.07) compared to 8.62 (95% CI 8.07-9.18) in placebo-treated controls (P < 0.001). The mean change of CCS at the end of treatment (day 8 versus [vs] day 0) was 6.04 in verum-treated vs 2.73 in placebo-treated controls (P < 0.001). Also 24 h defecation frequency (BSS) showed superior results (day 5: 0.84 vs 0.62, 95% CI 0.67-0.80, P < 0.001; day 6: 0.82 vs 0.60, 95% CI 0.64-0.78, P < 0.01 and day 8: 0.82 vs 0.60, 95% CI 0.64-0.78, P < 0.01) and better BSS type classification during treatment than controls (P < 0.05). No significant differences in adverse events between both groups became obvious. CONCLUSION: Rhubarb navel plaster administration over an 8-day-treatment period resulted in significantly improved bowel function as demonstrated by the CCS, 24 h defecating frequency and BSS. Our results suggest that rhubarb navel plasters represent a feasible, safe and efficient application route for the treatment of patients suffering from chronic constipation.

2.
Patient Educ Couns ; 103(5): 898-907, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-31810762

RESUMO

BACKGROUND: Cardiovascular disease has caused heavy health care burdens in many countries, and hypertension (HTN) is a well-known independent cardiovascular risk factor. OBJECTIVE: To assess the efficacy of empowerment strategies that affect systolic blood pressure (SBP), diastolic blood pressure (DBP), body mass index (BMI), quality of life, and self-management behaviours for patients with hypertension. METHODS: A literature search of the Cochrane Library, PubMed, MEDLINE, Embase, Web of Science, and several Chinese medical databases was performed. Study screening, quality assessment, data extraction, and meta-analysis were conducted according to Cochrane standards. RESULTS: Eleven randomised controlled trials with 988 subjects were identified. Relative to control groups, the empowerment strategies showed significant decreases in SBP (the mean difference [MD] = 9.46, 95 % confidence interval [CI] = 6.36-12.55, p <  0.00001) and DBP (MD = 6.68, 95 % CI = 3.07-10.29, p =  0.0003). However, no significant difference was found in BMI (p = 0.05). Subgroup analysis showed significant differences in the improvement of both SBP and DBP among the various groups, regardless of the duration and type of interventions. DISCUSSION: Empowerment strategies can decrease both SBP and DBP in hypertension patients. However, its influence on patients' BMI, quality of life, and self-management behaviour remains unclear. PRACTICAL VALUE: Empowerment strategies are useful for controlling the blood pressure of hypertension patients.

3.
Sci Total Environ ; 706: 135746, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31787306

RESUMO

The subtropical forest transition zone in southern China is a typical transition zone with high coverage and diverse vegetation. Projected climate change will affect physiological processes of trees, which would consequently alter the forest aboveground biomass (AGB) and composition at broad spatial scales. However, spatially heterogeneous responses may also be shaped by climate change, succession, and harvesting in different forest habitats. The objectives of this study were to assess the changes in subtropical forest AGB and composition in response to climate change, while comparing the responses of two similar forest landscapes: Taihe County (TH) and Longnan County (LN). We used a loose-coupling of PnET-II with LANDIS-II to simulate changes in forest AGB and composition under climate change scenarios (Current climate, RCP2.6, RCP4.5, RCP6.0, and RCP8.5) with harvest disturbances. Our simulation results demonstrated that forest AGB and composition were significantly affected by climate change in both landscapes. Changes in forest AGB was mostly driven by succession and harvest, but climate change also greatly contribute to the variation in AGB of deciduous broad-leaved forests (DBF), and coniferous forests (CF). Moreover, a larger area of LN experienced biomass reduction compared to TH, specifically under the RCP8.5 scenario. Given our estimates of the response in forest AGB and composition under climate change scenarios across different periods, we recommend that the regional forest management should be localized and should consider the effects of climate change through time in their planning schemes.


Assuntos
Mudança Climática , Florestas , Biomassa , China , Árvores
4.
Int J Biol Macromol ; 122: 1244-1252, 2019 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-30227201

RESUMO

α-Glucosidase is a critical enzyme associated with diabetes mellitus, and the inhibitors of the enzyme play important roles in the treatment of the disease. In this study, the inhibitory effect and mechanism of rifampicin on α-glucosidase were investigated by multispectroscopic methods along with molecular docking technique. The results showed that rifampicin inhibited α-glucosidase activity prominently (IC50 = 135 ±â€¯1.2 µM) in a reversible and competitive-type manner. The fluorescence intensity of α-glucosidase was quenched by rifampicin through forming rifampicin-α-glucosidase complex in a static procedure. And the formation of the rifampicin-α-glucosidase complex was driven spontaneously by hydrophobic forces and hydrogen bonds. The results obtained from molecular docking further indicated that hydrophobic forces were formed between rifampicin and amino acid residues Phe 173, Pro151, and hydrogen bonds were generated by the interactions of rifampicin with residues Ser 180, Asn 414, Gly160, and Gly161 of α-glucosidase. Moreover, it was found that the binding of rifampicin to α-glucosidase could alter the conformation of the enzyme to make it steady, and the binding distance was estimated to be 1.02 nm. Therefore, this study confirmed a novel α-glucosidase inhibitor and possibly contributed to the improvement of newfangled anti-diabetic agent.


Assuntos
Inibidores de Glicosídeo Hidrolases/metabolismo , Inibidores de Glicosídeo Hidrolases/farmacologia , Simulação de Acoplamento Molecular , Rifampina/metabolismo , Rifampina/farmacologia , alfa-Glucosidases/metabolismo , Sítios de Ligação , Transferência de Energia , Cinética , Conformação Proteica , Análise Espectral , Termodinâmica , alfa-Glucosidases/química
5.
Sci Total Environ ; 644: 762-769, 2018 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-29990924

RESUMO

Soil stores a substantial proportion of carbon (C), making it the greatest terrestrial C pool and pivotal to stabilizing the global climate system. Rainfall amounts and regimes have been changing in many places, but effects of precipitation changes on soil organic C (SOC) stabilization are not completely understood. Considerable attention has been focused on the consequences of changes in rainfall amounts, with rainfall regimes having been less studied. This study was conducted in a tropical climax forest to clarify the effects of rainfall changes on SOC fractions, with permanganate oxidation and density fractionations employed to divide the labile and non-labile SOC fractions. Two rainfall manipulation treatments, i.e., increased rainfall frequency with the total rainfall amount unchanged (IRF) and decreased rainfall amount by 50% with rainfall frequency unaltered (DRA), were conducted for two years, with ambient rainfall (AR) as the control. As a result, the IRF treatment increased the SOC concentration that mainly originated from increases in the non-labile SOC content. Relative to the AR control, the DRA treatment did not change the total SOC concentration although the labile SOC concentration increased. This typically is due to a small proportion of the labile fraction to the total SOC content. Our results suggest that this water-rich mature forest is resistant to rainfall amount changes to a great extent (e.g., decrease of 50% as in the present study) from the SOC stabilization perspective, while changes in rainfall frequency could exert more notable effects.


Assuntos
Carbono/análise , Monitoramento Ambiental , Florestas , Solo/química , Tempo (Meteorologia) , Clima
6.
Int J Biol Macromol ; 117: 538-545, 2018 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-29803745

RESUMO

Omeprazole was first evaluated for its antityrosinase activity and preservation of fresh-cut apples. The results obtained from enzymic analyses showed that the omeprazole inhibited tyrosinase activity (IC50 = 40 ±â€¯1.2 µM) with a reversible and competitive mechanism. Fluorescence quenching assays demonstrated that the interaction between omeprazole and tyrosinase was driven by hydrophobic forces and hydrogen bonds in a static procedure. Molecular docking further revealed that hydrogen bonds and hydrophobic forces were generated by omeprazole with the amino acid residues located in the A chain of tyrosinase. Moreover, the results from preservation assays showed that omeprazole could inhibit the activities of polyphenol oxidase (PPO) and peroxidase (POD), prevent the oxidation of total phenolics and flavonoid, thereby delay the browning of fresh-cut apples. Hence, this work identified a novel tyrosinase inhibitor and expands its feasible application as a food preservative.


Assuntos
Conservação de Alimentos/métodos , Malus/enzimologia , Monofenol Mono-Oxigenase/antagonistas & inibidores , Omeprazol/farmacologia , Agaricus/enzimologia , Catecol Oxidase/metabolismo , Di-Hidroxifenilalanina/metabolismo , Flavonoides/análise , Cinética , Malus/efeitos dos fármacos , Simulação de Acoplamento Molecular , Omeprazol/química , Peroxidase/metabolismo , Fenóis/análise , Soluções , Espectrometria de Fluorescência , Especificidade por Substrato
7.
Oncol Lett ; 15(6): 8261-8268, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29805560

RESUMO

Death associated protein kinase 1 (DAPK1) is a notable serine/threonine kinase involved in the regulation of multiple cellular pathways, including apoptosis and autophagy. Although DAPK1 is usually considered to be a tumor suppressor, it was previously reported to promote the viability of p53 mutant cancer cell lines and possess physiological oncogenic functions in breast cancer. However, the ability of endogenous DAPK1 to suppress breast cancer cell mobility has not been assessed. In the present study, the prognostic function of DAPK1 in a Chinese patient cohort was evaluated, and no significant association was observed between DAPK1 expression and patient survival or lymph node metastasis. In order to investigate the physiological function of endogenous DAPK1, stable inducible DAPK1 knockdown MCF7 and MDA-MB-231 cell lines were established. Consistent with previous studies, endogenous DAPK1 only regulated cell viability in p53 mutant MDA-MB-231 cells. However, knockdown of DAPK1 did not significantly affect cell motility of either MCF7 or MDA-MB-231 cells. Altogether, these results further explored the function of endogenous DAPK1 in breast cancer and may shed light in understanding the molecular signaling pathways regulating the physiological function of DAPK1.

8.
Food Chem ; 260: 27-36, 2018 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-29699669

RESUMO

This study investigated the structure, antioxidant activity, antityrosinase activity and mechanism of proanthocyanidins from mung bean seed [Vigna radiata (L.) Wilczek]. The structural composition were characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), electrospray ionization-full-mass spectrometry (ESI-Full-MS), and high-pressure liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) techniques. The mung bean seed proanthocyanidins were composed of procyanidins, prodelphinidins, and their rhamnosides. According to enzyme kinetic analysis, these compounds were potent, reversible, and mixed-type inhibitors of tyrosinase. They inhibited the enzyme activity by interacting with enzyme as well as substrates. The results of molecular docking showed that the interaction between mung bean seed proanthocyanidins and tyrosinase was driven by hydrogen bond, hydrophobic and electrostatic interactions. In addition, mung bean seed proanthocyanidins were demonstrated as powerful antioxidants. Therefore, this study confirmed a novel tyrosinase inhibitor and would lay a scientific foundation for their utilization in pharmaceutical and food industries.


Assuntos
Antioxidantes/farmacologia , Inibidores Enzimáticos/farmacologia , Monofenol Mono-Oxigenase/antagonistas & inibidores , Proantocianidinas/farmacologia , Vigna/química , Antioxidantes/química , Cromatografia Líquida de Alta Pressão/métodos , Inibidores Enzimáticos/química , Cinética , Simulação de Acoplamento Molecular , Estrutura Molecular , Monofenol Mono-Oxigenase/química , Monofenol Mono-Oxigenase/metabolismo , Proantocianidinas/química , Sementes/química , Sementes/metabolismo , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
9.
J Agric Food Chem ; 66(4): 908-917, 2018 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-29313327

RESUMO

In this study, the content, structure, antityrosinase activity, and mechanism of longan bark condensed tannins were evaluated. The findings obtained from mass spectrometry demonstrated that longan bark condensed tannins were mixtures of procyanidins, propelargonidins, prodelphinidins, and their acyl derivatives (galloyl and p-hydroxybenzoate). The enzyme analysis indicated that these mixtures were efficient, reversible, and mixed (competitive is dominant) inhibitor of tyrosinase. What's more, the mixtures showed good inhibitions on proliferation, intracellular enzyme activity and melanogenesis of mouse melanoma cells (B16). From molecular docking, the results showed the interactions between inhibitors and tyrosinase were driven by hydrogen bond, electrostatic, and hydrophobic interactions. In addition, high levels of total phenolic and extractable condensed tannins suggested that longan bark might be a good source of tyrosinase inhibitor. This study would offer theoretical basis for the development of longan bark condensed tannins as novel food preservatives and medicines of skin diseases.


Assuntos
Inibidores Enzimáticos/farmacologia , Monofenol Mono-Oxigenase/antagonistas & inibidores , Casca de Planta/química , Sapindaceae/química , Taninos/química , Taninos/farmacologia , Animais , Antocianinas/farmacologia , Biflavonoides/farmacologia , Catequina/farmacologia , Proliferação de Células/efeitos dos fármacos , Ligação de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Espectrometria de Massas , Melaninas/análise , Melaninas/antagonistas & inibidores , Melaninas/biossíntese , Melanoma Experimental , Camundongos , Modelos Moleculares , Simulação de Acoplamento Molecular , Oxirredutases , Parabenos/farmacologia , Proantocianidinas/farmacologia , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Eletricidade Estática , Relação Estrutura-Atividade
10.
Food Res Int ; 100(Pt 1): 731-739, 2017 10.
Artigo em Inglês | MEDLINE | ID: mdl-28873743

RESUMO

In this study, the structure of proanthocyanidins purified from cherimoya (Annona squamosa) pericarp was analyzed by ESI-QTOF-MS and HPLC analyses. The result indicated that these compounds were procyanidin-type proanthocyanidins, consisting mainly of (epi)catechin units linked b y B-type interflavan bonds. The analyses of enzymology showed that the activities of monophenolase and diphenolase of tyrosinase could be powerfully inhibited by the proanthocyanidins. Further researches on the inhibition mechanism demonstrated that they were reversible and competitive inhibitors with the KI value of 27.1±3.1µg/mL. These inhibitors quenched the fluorescence of tyrosinase through a static quenching mechanism and spontaneously formed proanthocyanidins-enzyme complex. Fluorescence changes of proanthocyanidins in the presence of copper ion suggested that the interactions could reduce the fluorescence intensity of these polymers and the molecular docking analysis revealed that copper irons of the enzyme could be chelated by adjacent hydroxyl groups on the B ring of proanthocyanidins. Moreover, proanthocyanidins were proved to be efficient quencher of substrates. These results would lay scientific foundation for their farther application in food and medicine industry.


Assuntos
Annonaceae/química , Inibidores Enzimáticos/química , Monofenol Mono-Oxigenase/antagonistas & inibidores , Monofenol Mono-Oxigenase/química , Proantocianidinas/química , Inibidores Enzimáticos/metabolismo , Inibidores Enzimáticos/farmacologia , Frutas/química , Simulação de Acoplamento Molecular , Monofenol Mono-Oxigenase/metabolismo , Proantocianidinas/metabolismo , Proantocianidinas/farmacologia , Espectrometria de Fluorescência , Espectrometria de Massas por Ionização por Electrospray
11.
Int J Biol Macromol ; 102: 425-430, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28414110

RESUMO

In this study, the inhibitory effect and mechanism of rifampicin on the activity of tyrosinase were investigated for developing a novel tyrosinase inhibitor. It was found to have a significant inhibition on the activity of tyrosinase (IC50=90±0.6µM). From the kinetics analysis, it was proved to be a reversible and noncompetitive type inhibitor of the enzyme with the KI value of 94±3.5µM. The results obtained from intrinsic fluorescence quenching indicated that rifampicin could interact with tyrosinase. In particular, the drastic decrease of fluorescence intensity was due to the formation of a rifampicin-enzyme complex in a static procedure which was mainly driven by hydrophobic forces and hydrogen bonding. Moreover, the ANS-binding fluorescence analysis suggested that rifampicin binding to tyrosinase changed the polarity of the hydrophobic regions. Molecular docking analysis further revealed that the hydrogen bonds were generated between rifampicin and amino residues Leu7, Ser52, and Glu107 in the B chain of the enzyme. And the hydrophobic forces produced through the interaction of rifampicin with B chain residues Pro9, Pro14, and Trp106. This work identified a novel tyrosinase inhibitor and potentially contributed to the usage of rifampicin as a potential hyperpigmentation drug.


Assuntos
Inibidores Enzimáticos/farmacologia , Monofenol Mono-Oxigenase/antagonistas & inibidores , Rifampina/farmacologia , Agaricales/enzimologia , Inibidores Enzimáticos/metabolismo , Cinética , Simulação de Acoplamento Molecular , Monofenol Mono-Oxigenase/química , Monofenol Mono-Oxigenase/metabolismo , Conformação Proteica , Rifampina/metabolismo
12.
Food Funct ; 8(3): 1043-1051, 2017 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-28128839

RESUMO

Fruit pericarp of Clausena lansium (Lour.) Skeels, a food waste, was selected as a raw material for proanthocyanidins. The proanthocyanidins' structures were integrally analyzed using three methods: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), high performance liquid chromatography electrospray ionization mass spectrometry (HPLC-ESI-MS) and 13C nuclear magnetic resonance (NMR). The results elucidated that these compounds were composed of prodelphinidin (75%) and procyanidin (25%) with a degree of polymerization (DP) up to the 20-mers. They were proved to be remarkable, reversible and mixed competitive inhibitors of tyrosinase according to results from enzyme experiments. The IC50 values were calculated to be 23.6 ± 1.2 and 7.0 ± 0.2 µg mL-1 for the monophenolase and diphenolase activities, respectively. In addition, the proanthocyanidins had a good inhibitory effect on cell proliferation, cellular tyrosinase activity and melanin production of B16 mouse melanoma cells. Chelation between the hydroxyl group on the B ring of the proanthocyanidins and dicopper irons of the enzyme provided one of the feasible mechanisms for the inhibition on the basis of fluorescence quenching and molecular docking analyses. This research would supply the scientific basis to these compounds application in the pharmaceutical, insecticides, and preservative fields.


Assuntos
Clausena/química , Inibidores Enzimáticos/química , Frutas/química , Monofenol Mono-Oxigenase/antagonistas & inibidores , Extratos Vegetais/química , Proantocianidinas/química , Animais , Linhagem Celular , Sobrevivência Celular , Células/efeitos dos fármacos , Células/enzimologia , Cromatografia Líquida de Alta Pressão , Inibidores Enzimáticos/isolamento & purificação , Inibidores Enzimáticos/farmacologia , Cinética , Camundongos , Estrutura Molecular , Monofenol Mono-Oxigenase/química , Monofenol Mono-Oxigenase/metabolismo , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia , Proantocianidinas/isolamento & purificação , Proantocianidinas/farmacologia , Espectrometria de Massas por Ionização por Electrospray
13.
Sci Rep ; 6: 37026, 2016 11 15.
Artigo em Inglês | MEDLINE | ID: mdl-27845388

RESUMO

Rain and dew droplets can dislodge or drown small insects and may be important factors that drive adaptations for avoidance and escape. Studying the microstructure of small insects and insect behaviour can help understand these adaptations. We quantified avoidance behaviour and entrapment of nymphs and adults of the tea green leafhopper (Empoasca onukii) using simulated rainfall onto host plant foliage and made observations of pretarsi and abdomen microstructures. Adults responded rapidly to simulated rainfall and escaped by jumping whilst most young nymphs were washed from water-sprayed leaves though older nymphs tended to remain on leaves and subsequently escaped from water droplets. Adults had denser covering of water-repelling brochosomes on pretarsi and abdomen surface than nymphs, and were able to stand on water film whilst most nymphs had multiple penetrating tarsi. Removal of brochosomes from the abdomen of adults reduced hydrophobicity, demonstrating the hydrophobic significance of brochosomes in the capacity of leafhopper to escape from water droplets. Nymphs exhibited a higher pull-off force than adults. This research is one of the few studies to focus on the wettability and water avoidance of small insect pests and has implications for pest management.


Assuntos
Adaptação Fisiológica/fisiologia , Aprendizagem da Esquiva/fisiologia , Comportamento Animal/fisiologia , Hemípteros/fisiologia , Chuva , Animais
14.
PLoS One ; 11(3): e0150298, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26950337

RESUMO

BACKGROUND AND PURPOSE: Stroke-associated pneumonia (SAP) is a common complication and an important cause of death during hospitalization. The A2DS2 (Age, Atrial fibrillation, Dysphagia, Sex, Stroke Severity) score was developed from the Berlin Stroke Registry and showed good predictive value for predicting SAP. We sought to identify the association between the A2DS2 score and SAP, and, furthermore, to identify whether the A2DS2 score was a predictor for in-hospital death after acute ischemic stroke in a Chinese population. METHODS: This was a retrospective study. 1239 acute ischemic stroke patients were classified to low A2DS2 group (0-4) and high A2DS2 score (5-10) group. Primary outcome was in-hospital SAP. Logistic regression analyses were performed to identify the association between the A2DS2 score and SAP, and also the association between the A2DS2 score and in-hospital death. RESULTS: The overall incidence rates of SAP and in-hospital mortality after acute ischemic stroke were 7.3% and 2.4%, respectively. The incidence rate of SAP in low and high A2DS2 score groups was separately 3.3% and 24.7% (P<0.001). During hospitalization, 1.2% patients in low score group and 7.8% patients in high score group died (P<0.001). Multivariate regression demonstrated that patients in high score group had a higher risk of SAP (OR = 8.888, 95%CI: 5.552-14.229) and mortality (OR = 7.833, 95%CI: 3.580-17.137) than patients in low score group. CONCLUSIONS: The A2DS2 score was a strong predictor for SAP and in-hospital death of Chinese acute ischemic stroke patients. The A2DS2 score might be a useful tool for the identification of patients with a high risk of SAP and death during hospitalization.


Assuntos
Fibrilação Atrial/complicações , Isquemia Encefálica/complicações , Transtornos de Deglutição/complicações , Mortalidade Hospitalar , Pneumonia/complicações , Pneumonia/diagnóstico , Acidente Vascular Cerebral/mortalidade , Fatores Etários , Idoso , Feminino , Humanos , Masculino , Prognóstico , Estudos Retrospectivos , Fatores de Risco , Fatores Sexuais , Acidente Vascular Cerebral/complicações
15.
Sheng Wu Gong Cheng Xue Bao ; 32(8): 1133-1144, 2016 Aug 25.
Artigo em Chinês | MEDLINE | ID: mdl-29022314

RESUMO

The expression of dentin sialophosphoprotein (DSPP) is the marker for cells differentiated into odontoblasts. This study attempted to analyze the DSPP promoter and build the reporter LacZ expression system driven by this promoter, which allows convenient and quick detection of odontoblast cells. First, we separated the human dental mesenchymal cells in which the expression of DSPP can be effectively induced by dexamethasone. Second, four 5' flanking regions of human DSPP gene (- 4 000-+54, -2 500-+54, -1 447-+54 and -1 027-+54) were analyzed, the results showed that the highest promoter activity lied in the -2 500-+54 region. The promoter activity is reduced when the 5' flanking region was extended from -2 500 to -4 000 bp upstream from the transcription start site; The promoter activity are also decreased when the 5' flanking regions were shorted from -2 500 to -1 447 bp and from -1 447 to -1 027 bp, indicating that potential suppresser elements are lied in the region between -4 000 and -2 500 bp and potential activator elements are lied in the region between -2 500 and -1 027 bp. Then we constructed the lentiviral report vector phDSPP-LacZ containing the -2 500-+ 54 promoter region in front of the LacZ gene. The expression of LacZ was detected using X-Gal staining in both human dental mesenchymal cells and immortalized human dental mesenchymal cells infected with phDSPP-LacZ. The phDSPP-LacZ lentiviral vector may provide a more convenient method to detect the expression of DSPP in human odontogenic differentiation, tooth development and tooth regeneration studies.


Assuntos
Proteínas da Matriz Extracelular/genética , Óperon Lac , Odontoblastos/citologia , Fosfoproteínas/genética , Regiões Promotoras Genéticas , Sialoglicoproteínas/genética , Diferenciação Celular , Genes Reporter , Humanos
16.
BMC Musculoskelet Disord ; 16: 161, 2015 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-26148546

RESUMO

BACKGROUND: The Short Musculoskeletal Function Assessment (SMFA) questionnaire is one of the most commonly used scales to evaluate functional status and quality of life (QOL) of patients with a broad range of musculoskeletal disorders. However, a Chinese version of the SMFA questionnaire for the psychometric properties of skeletal muscle injury patients in China is still lacking. The current study translated the SMFA into Chinese and assessed its reliability and validity among Chinese patients with skeletal muscle injury of the upper or lower extremities. METHODS: The original SMFA was translated from English into Chinese and culturally adapted according to cross-cultural adaptation guidelines. A multicenter cross-sectional study was conducted, comprising 339 skeletal muscle injury patients (aged 20-75 years) from 4 hospitals. The SMFA, the health survey short form (SF-36) along with a region-specific questionnaire (including the disabilities of the arm, shoulder, and hand questionnaire (DASH), the hip disability and osteoarthritis outcome score (HOOS), the knee injury and osteoarthritis outcome score (KOOS), and the foot function index (FFI)) were completed according to the region of injury. Reliability was estimated from the internal consistency using Cronbach's α and validity was assessed via convergent validity, known-groups comparison, and construct validity. RESULTS: Cronbach's α coefficient was over 0.75 for two subscales and four categories of the SMFA, suggesting that the internal consistency reliability of the SMFA was satisfactory. Known-groups comparison showed that the dysfunction index and the bother index of the SMFA discriminated well between patients who differed in age, gender, injury location, and operation status rather than in subgroups based on the body mass index (BMI). The convergent validity of the SMFA was good, as moderate to excellent correlations were found between the subscales of the SMFA and the four subscales of SF-36 (physical function, role-physical, bodily pain, and social functioning) and the region-specific questionnaires. The construct validity was proved by the presence of a six-factor structure that accounted for 66.85 % of the variance. CONCLUSION: The Chinese version of the SMFA questionnaire is a reliable and valid instrument to measure patient-reported impact of musculoskeletal injuries in the upper or lower extremities.


Assuntos
Traumatismos do Braço/diagnóstico , Traumatismos da Perna/diagnóstico , Músculo Esquelético/lesões , Doenças Musculoesqueléticas/diagnóstico , Inquéritos e Questionários , Atividades Cotidianas , Adulto , Idoso , Traumatismos do Braço/etnologia , Traumatismos do Braço/fisiopatologia , Traumatismos do Braço/psicologia , Grupo com Ancestrais do Continente Asiático/psicologia , China , Efeitos Psicossociais da Doença , Estudos Transversais , Características Culturais , Avaliação da Deficiência , Emoções , Estudos de Viabilidade , Feminino , Nível de Saúde , Humanos , Traumatismos da Perna/etnologia , Traumatismos da Perna/fisiopatologia , Traumatismos da Perna/psicologia , Masculino , Pessoa de Meia-Idade , Músculo Esquelético/fisiopatologia , Doenças Musculoesqueléticas/etnologia , Doenças Musculoesqueléticas/fisiopatologia , Doenças Musculoesqueléticas/psicologia , Valor Preditivo dos Testes , Psicometria , Qualidade de Vida , Reprodutibilidade dos Testes , Tradução , Adulto Jovem
17.
J Dent ; 43(5): 576-82, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25687752

RESUMO

OBJECTIVES: Due to the rarity of human embryonic samples and limited proliferating capability of primary human dental mesenchymal cells, it is valuable to create an immortalized human dental mesenchymal cell line for studying dental mesenchymal cell differentiation and signalling pathways during dentinogenesis in humans. METHODS: In this study, dental mesenchymal cells from human molar tooth germs at 19-week gestation were isolated and immortalized with pSV40. Single cell colonies were then selected by 96-well plate dilution. The immortalized cell line was characterized using immunofluorescent microscopy, RT-PCR and Western blot for the expression of SV40 large T antigen and five genes specific for the mesenchymal stage during tooth development. The differentiation and mineralization activities of the immortalized and primary cells were compared using adipogenic and calcifying induction. RESULTS: The immortalized dental mesenchymal cell line displayed a higher proliferation rate, expressed several tooth-specific markers including Msx1, Pax9, Lhx6, Barx1, and Runx2, and maintained the ability to differentiate and form mineralized nodules. CONCLUSIONS: Our results demonstrated that the immortalized human mesenchymal cell line retained the characteristics similar to primary human dental mesenchymal cells and can be used for studying the mechanisms of human dental mesenchymal cell differentiation and signalling pathways involved in human odontogenesis.


Assuntos
Células-Tronco Mesenquimais/citologia , Mesoderma/citologia , Germe de Dente/citologia , Feto Abortado/citologia , Calcificação Fisiológica , Diferenciação Celular/fisiologia , Linhagem Celular , Proliferação de Células/fisiologia , Células Cultivadas , Humanos , Dente Molar/citologia , Germe de Dente/metabolismo
18.
Zhongguo Zhong Yao Za Zhi ; 36(17): 2325-8, 2011 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-22121795

RESUMO

OBJECTIVE: To establish an effective protocol for plant generation and induce polyploidy of Morinda offcinalis. METHOD: Callus was induced from immature embryo of M. officinalis and polyploidy was inducted by using colchicine treatment method. Chromosome was detected by flow cytometry. RESULT AND CONCLUSION: The highest induction rate of polyploidy was 18.40%, which was obtained with 500 mg x L(-1) colchicine treatment for 5 days. Roots of polyploid were bigger than diploid. Advantages of using immature embryo as explants are easy for sterilization, higher rate of callus induction and low degree dedifferentiation. The induced polyploidy of M. officinalis may have a value for spread of cultivation.


Assuntos
Morinda/crescimento & desenvolvimento , Morinda/genética , Poliploidia , Técnicas de Cultura de Tecidos/métodos , Cromossomos de Plantas/genética
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