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1.
Chin J Nat Med ; 18(1): 75-80, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31955826

RESUMO

Purpurolides D-F (1-3), three new polyoxygenated bergamotanes bearing a 6/4/5/5 tetracyclic ring system, were isolated from the endophytic fungus Penicillium purpurogenum IMM 003. Their structures were unambiguously elucidated based on extensive spectroscopic data analyses, 13C NMR chemical shifts calculations coupled with the DP4+ probability method, and the calculated and experimental electronic circular dichroism (ECD) spectra. Compounds 1-3 showed significant inhibitory activity against pancreatic lipase (PL). The result highlights that the presence of 3-hydroxylated decanoic acid moiety at C-14 is important for increasing the inhibition potency against PL.

2.
Biol Proced Online ; 21: 23, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31798349

RESUMO

Background: Lysine post-translational modifications are important regulators of protein function. Proteomic and biochemical approaches have resulted in identification of several lysine modifications, including acetylation, crotonylation, and succinylation. Here, we developed an approach for surveying amide-bonded lysine modifications in the proteome of human tissues/cells based on the observation that many lysine modifications are amide-bonded and that the Salmonella enterica deacetylase, CobB, is an amidase. Results: After the proteome of human tissues/cells was denatured and the non-covalently bonded metabolites were removed by acetone washes, and the amide-bonded modifiers were released by CobB and analyzed using liquid- and/or gas chromatography/mass spectrometry metabolomic analysis. This protocol, which required 3-4 days for completion, was used to qualitatively identify more than 40 documented and unreported lysine modifications from the human proteome and to quantitatively analyze dynamic changes in targeted amide-bonded lysine modifications. Conclusions: We developed a method that was capable of monitoring and quantifying amide-bonded lysine modifications in cells of different origins.

3.
J Asian Nat Prod Res ; : 1-8, 2019 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-31833397

RESUMO

Three new polyketides including two benzophenone derivatives, penibenzones A and B (1 and 2), and a new phthalide derivative, penibenzone C (3), along with six known compounds, were isolated from the solid-substrate cultures of the endophytic fungus Penicillium purpurogenum IMM003. Their structures were elucidated on the basis of spectroscopic data (UV, IR, HRESIMS, 1D and 2D NMR). All compounds were evaluated for inhibitory activity against pancreatic lipase, but none of them displayed activity.

4.
Zhongguo Zhong Yao Za Zhi ; 44(20): 4538-4544, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31872643

RESUMO

Through using the theoretical research results of traditional Chinese medicine resource management and sustainable utilization as references,this article systematically analyzes the relationship between economic subsystems for utilization of wild renewable resources in Tibetan medicine,resources,environment and social systems. It sorts out and designs the relationship diagram of the sustainable utilization system for Tibetan medicine resources,input and output relationship diagrams of economics subsystems for Tibetan medicine resources,development model diagram for Tibetan medical industrial chain,market development flow chart of wild herb resources of Tibetan medicine,causal circuit diagram of sustainable use mechanism for Tibetan medicine resources,cause tree of the stock and function for Tibetan medicine resources,cause tree of total income for Tibetan medicine resource utilization and tree diagram of compensation fund usage of Tibetan medicine resources. It provides an innovative research method for Tibetan medicine resources,and theoretical basis and relevant countermeasure for constructing sustainable approaches to development of wild renewable resources in Tibetan medicine.


Assuntos
Medicina Tradicional Tibetana , Plantas Medicinais , Medicina Tradicional Chinesa , Desenvolvimento Sustentável , Tibet
5.
Zhongguo Zhong Yao Za Zhi ; 44(22): 4864-4873, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31872594

RESUMO

Fifteen limonoids were isolated from 95% ethanol extracts of the dry seeds of neem( Azadirachta indica) by various column chromatography techniques including silica gel,Pharmadex LH-20 gel and ODS resin. Based on spectroscopic analysis,their structures were determined as nimbocinol( 1),17ß-hydroxynimbocinol( 2),1α,3α,7α-triacetylvilasinin( 3),7α-benzoyltrichilinin( 4),1,3-diacetyl-7-tigloyl-12-hydroxyvilasinin( 5),3-deacetylsalannin( 6),1-O-acetyl-1-detigloylsalannin( 7),2'( R),3'-dihydrosalannin( 8),2'( S),3'-dihydrosalannin( 9),2,3-dihydronimbolide( 10),6-homodesacetylnimbin( 11),gedunin( 12),7-deacetyl-7-epi-dihydrogedunin( 13),7-deacetoxy-7α-hydroxygedunin( 14) and nimbinene( 15). Compound 7 is a new natural product. 4,8,9,13 and 14 are isolated from the genus Azadirachta for the first time. Compound 2 showed inhibitory activity against Escherichia coli and Staphylococcus epidermidis,with MIC values of 32 and 128 mg·L~(-1),respectively. Compound 10 showed moderate inhibitory activity against S. epidermidis with a MIC value of 64 mg·L~(-1). Compound 11 inhibited the growth of E. coli and Pseudomonas aeruginosa,both with MIC values of 128 mg·L~(-1). Compound 15 exhibited inhibitory activity against P. aeruginosa,with a MIC value of128 mg·L~(-1).


Assuntos
Antibacterianos/farmacologia , Azadirachta , Limoninas , Extratos Vegetais/farmacologia , Escherichia coli , Sementes
6.
Infect Drug Resist ; 12: 3189-3195, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31632104

RESUMO

Background: Patients with high-titer anti-IFN-γ autoantibodies present disseminated non-tuberculous mycobacterial (NTM) and other opportunistic infections. Due to its rare occurrence and non-specific symptoms, this syndrome is difficult to diagnose during early disease stages. Here, we report a case with high-concentrations of serum anti-IFN-γ autoantibodies who presented with disseminated Talaromyces marneffei and NTM disease accompanied Sweet's syndrome. Case presentation: A 62-year-old Chinese woman with no previous history was admitted to our hospital in August 2016 due to intermittent fever for 2 years, left chest wall redness, and swelling for 3 months. During hospitalization, the patient was confirmed with disseminated T. marneffei and successfully treated with antifungal therapy. In July 2017, upon second admission, Mycobacterium avium intracellular (MAC) pulmonary infection was established after positive cultures from the right lung tissue. The patient failed treatment after 1 month of anti-NTM therapy due to side effects. In May 2018, she was confirmed as having disseminated MAC disease accompanied by hand rashes, which was considered as Sweet's syndrome. High-level anti-IFN-γ antibodies in the patient serum were detected upon comparison with normal controls (2.85-fold increase). Following anti-NTM therapy, both symptoms and pulmonary infiltration gradually improved, and joint destruction and lymphadenitis remained. Conclusions: Patients with anti-interferon-γ autoantibodies should be considered for severe, recurrent infections in adults in the absence of other known risk factors. Sweet's syndrome is a common skin manifestation of the syndrome.

7.
Nat Commun ; 10(1): 2502, 2019 06 07.
Artigo em Inglês | MEDLINE | ID: mdl-31175280

RESUMO

Accumulation of nucleotide building blocks prior to and during S phase facilitates DNA duplication. Herein, we find that the anaphase-promoting complex/cyclosome (APC/C) synchronizes ribose-5-phosphate levels and DNA synthesis during the cell cycle. In late G1 and S phases, transketolase-like 1 (TKTL1) is overexpressed and forms stable TKTL1-transketolase heterodimers that accumulate ribose-5-phosphate. This accumulation occurs by asymmetric production of ribose-5-phosphate from the non-oxidative pentose phosphate pathway and prevention of ribose-5-phosphate removal by depleting transketolase homodimers. In the G2 and M phases after DNA synthesis, expression of the APC/C adaptor CDH1 allows APC/CCDH1 to degrade D-box-containing TKTL1, abrogating ribose-5-phosphate accumulation by TKTL1. TKTL1-overexpressing cancer cells exhibit elevated ribose-5-phosphate levels. The low CDH1 or high TKTL1-induced accumulation of ribose-5-phosphate facilitates nucleotide and DNA synthesis as well as cell cycle progression in a ribose-5-phosphate-saturable manner. Here we reveal that the cell cycle control machinery regulates DNA synthesis by mediating ribose-5-phosphate sufficiency.


Assuntos
Ciclossomo-Complexo Promotor de Anáfase/metabolismo , Proteínas Cdh1/metabolismo , Ciclo Celular , Replicação do DNA , Ribosemonofosfatos/metabolismo , Transcetolase/metabolismo , Proteínas Cdc20/metabolismo , Proteínas de Ciclo Celular/metabolismo , Divisão Celular , Fase G2 , Humanos , Via de Pentose Fosfato , Fase S
8.
Chin J Nat Med ; 17(1): 22-26, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30704619

RESUMO

Guided by TNF-α secretion inhibitory activity assay, four taraxastane-type triterpenoids, including two new ones, 22-oxo-20-taraxasten-3ß, 30-diol (1) and 22α-hydroxy-20-taraxasten-30ß, 30-triol (2), have been obtained from an active fraction of the petroleum ether-soluble extract of the the medicinal and edible plant Cirsium setosum. Their structures were elucidated by spectroscopic data and CD data analysis. In the TNF-α secretion inhibitory activity assay, compounds 1 and 2 were active with the IC50 of 2.6 and 3.8 µmol·L-1, respectively. In addition, compounds 1 and 2 showed moderately selective cytotoxicity against the human ovarian cancer (A2780) and colon cancer (HCT-8) cell lines.


Assuntos
Cirsium/química , Plantas Comestíveis/química , Plantas Medicinais/química , Triterpenos/química , Animais , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Éter/química , Humanos , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Camundongos , Estrutura Molecular , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Triterpenos/isolamento & purificação , Triterpenos/farmacologia , Fator de Necrose Tumoral alfa/metabolismo
9.
Infect Drug Resist ; 11: 2483-2490, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30555247

RESUMO

Background: Clinicians may fail to make an early diagnosis of pulmonary cryptococcosis (PC) without HIV infection. Serum cryptococcal capsular polysaccharide antigen (CrAg) test, histopathology and culture of lung tissue play different roles in diagnosis of PC. Objective: To investigate the performance of serum CrAg test, histopathology and culture of the lung tissue in diagnosis of PC without HIV infection. Patients/methods: From January 2011 to September 2017, patients with proven PC were recruited from a teaching hospital in southern China. Those patients with HIV infection, PC confirmed by surgery or PC with probable or possible diagnosis were excluded from the study. Latex agglutination test and CrAg lateral flow assay were used for detection of serum CrAg. Lung biopsy and needle aspiration were performed under computed tomography guidance. Results: Eighty-nine patients with proven PC including 41 male (46.1%) and 48 female (53.9%) were enrolled. Fifty-one (57.3%) patients had underlying disease. Positive CrAg test was found in 83 (93.3%) cases. Among six cases with negative CrAg test, PC was confirmed by histology in two cases and positive culture in four cases. The histopathological results of 77 (86.5%) cases revealed cryptococcal granuloma and 12 cases showed chronic inflammation, which was confirmed by positive culture. Among 65 cases, the diseased tissue of 46 (70.8%) cases presented Cryptococcus neoformans in the culture and one case was diagnosed with lung cancer coexisting with PC. Conclusion: Our findings showed that serum CrAg test is rapid and sensitive in diagnosing PC, histology is important for confirming PC and culture plays a complementary role. Biopsied lung tissue should be submitted for cultures whenever feasible.

10.
Zhongguo Zhong Yao Za Zhi ; 43(16): 3315-3321, 2018 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-30200735

RESUMO

Dihydrochelerythrine was isolated from the ethanol extract of Corydalis yanhusuo by chromatographic and recrystallization techniques. To our knowledge, this is the first report that dihydrochelerythrine was found to be unstable. The NMR, HPLC, and LC-MS were applied to monitor the structural conversion process of dihydrochelerythrine. The results showed that when dissolved in polar deuteration solvent (e.g., DMSO-d6 & MeOD), dihydrochelerythrine is directly converted to chelerythrine gradually. However, if used non-polar reagent (e.g.,CD2Cl2), the sample of dihydrochelerythrine undergoes the formation of pseudobase, chelerythrine, and bimolecular ether then followed by oxidation to oxychelerythrine as the major final product. Which leads to this phenomenon maybe is that the C-6 in dihydrochelerythrine is highly reactive to nucleophiles, and is easily converted to different derivatives in different solvents attributed to the solvent effect. This finding will contribute to the extraction and isolation, bioactivity screening, and quality evaluation of medicinal materials containing dihydrochelerythrine and other similar derivatives.


Assuntos
Benzofenantridinas/química , Corydalis/química , Extratos Vegetais/química , Solventes/química , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas em Tandem
11.
Eur J Med Chem ; 145: 717-725, 2018 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-29353723

RESUMO

Bysspectin A (1), a polyketide-derived octaketide dimer with a novel carbon skeleton, and two new precursor derivatives, bysspectins B and C (2 and 3), were obtained from an organic extract of the endophytic fungus Byssochlamys spectabilis that had been isolated from a leaf tissue of the traditional Chinese medicinal plant Edgeworthia chrysantha, together with a known octaketide, paecilocin A (4). Their structures were determined by HRMS, 1D and 2D NMR spectroscopic analysis. A plausible route for their biosynthetic pathway is proposed. Compounds 1-3 were tested for their antimicrobial activities. Only compound 3 was weakly active against Escherichia coli and Staphyloccocus aureus with MIC values of 32 and 64 µg/mL, respectively. Further, the inhibitory effects on human carboxylesterases (hCE1, hCE2) of compounds 1 and 4 were evaluated. The results demonstrated that bysspectin A (1) was a novel and highly selective inhibitor against hCE2 with the IC50 value of 2.01 µM. Docking simulation also demonstrated that active compound 1 created interaction with the Ser-288 (the catalytic amino-acid in the catalytic cavity) of hCE2 via hydrogen bonding, revealing its highly selective inhibition toward hCE2.


Assuntos
Antibacterianos/farmacologia , Byssochlamys/química , Carboxilesterase/antagonistas & inibidores , Hidrolases de Éster Carboxílico/antagonistas & inibidores , Inibidores Enzimáticos/farmacologia , Escherichia coli/efeitos dos fármacos , Policetídeos/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Antibacterianos/química , Antibacterianos/isolamento & purificação , Biocatálise , Carboxilesterase/metabolismo , Hidrolases de Éster Carboxílico/metabolismo , Dimerização , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/química , Inibidores Enzimáticos/isolamento & purificação , Humanos , Testes de Sensibilidade Microbiana , Simulação de Acoplamento Molecular , Estrutura Molecular , Policetídeos/química , Policetídeos/isolamento & purificação , Relação Estrutura-Atividade
12.
Cell Metab ; 27(1): 151-166.e6, 2018 01 09.
Artigo em Inglês | MEDLINE | ID: mdl-29198988

RESUMO

Amino acids are known regulators of cellular signaling and physiology, but how they are sensed intracellularly is not fully understood. Herein, we report that each aminoacyl-tRNA synthetase (ARS) senses its cognate amino acid sufficiency through catalyzing the formation of lysine aminoacylation (K-AA) on its specific substrate proteins. At physiologic levels, amino acids promote ARSs bound to their substrates and form K-AAs on the ɛ-amine of lysines in their substrates by producing reactive aminoacyl adenylates. The K-AA marks can be removed by deacetylases, such as SIRT1 and SIRT3, employing the same mechanism as that involved in deacetylation. These dynamically regulated K-AAs transduce signals of their respective amino acids. Reversible leucylation on ras-related GTP-binding protein A/B regulates activity of the mammalian target of rapamycin complex 1. Glutaminylation on apoptosis signal-regulating kinase 1 suppresses apoptosis. We discovered non-canonical functions of ARSs and revealed systematic and functional amino acid sensing and signal transduction networks.


Assuntos
Aminoacilação , Espaço Intracelular/metabolismo , Lisina/metabolismo , Transdução de Sinais , Aminoacil-tRNA Sintetases/metabolismo , Apoptose , Biocatálise , Células HEK293 , Humanos , Alvo Mecanístico do Complexo 1 de Rapamicina/metabolismo , Especificidade por Substrato
13.
J Asian Nat Prod Res ; 20(12): 1129-1136, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30621451

RESUMO

A new sesquiterpenoid ester glycoside (1) and a new monoterpenoid ester glycoside (2) have been isolated from an ethanol extract of the twigs of Litsea cubeba. Their structures were elucidated by extensive 1D- and 2D-NMR experiments, and the absolute configurations were determined by chemical methods, specific rotation, and a combination of experimental and theoretically calculated electronic circular dichroism spectra. Compound 1 exhibited selective cytotoxicity against A549 and HCT-8 cell lines with the IC50 values of 8.9 and 9.6 µM, respectively.


Assuntos
Glicosídeos/química , Litsea/química , Terpenos/química , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Linhagem Celular Tumoral , Humanos , Estrutura Molecular
14.
J Asian Nat Prod Res ; 20(11): 1093-1100, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30595053

RESUMO

A new oxacyclododecindione-type macrolactone, (13R,14S,15R)-13-hydroxy-14-deoxyoxacyclododecindione (1), has been obtained from the solid cultures of the fungus Exserohilum rostratum, a fungal strain endophytic in Gymnadenia conopsea. Its structure, including the absolute configuration, was extensively established by 1D and 2D NMR data, the modified Mosher method, and a combination of experimental and theoretically calculated electronic circular dichroism (ECD) spectra. Compound 1 showed weak selective cytotoxicity against the A549 lung cell line with an IC50 value of 9.2 µM.


Assuntos
Ascomicetos/química , Endófitos/fisiologia , Lactonas/farmacologia , Orchidaceae/microbiologia , Células A549 , Antineoplásicos/química , Antineoplásicos/farmacologia , Ascomicetos/fisiologia , Sobrevivência Celular/efeitos dos fármacos , Humanos , Lactonas/química , Estrutura Molecular
15.
J Pharm Biomed Anal ; 129: 210-218, 2016 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-27429371

RESUMO

Molecular weights and contents of water-soluble polysaccharides and their fractions in fifty batches of fruits of Lycium barbarum (wolfberry) collected from different regions of China, including Qinghai, Ningxia, Inner Mongolia, Xinjiang, and Gansu, were simultaneously determined using high performance size exclusion chromatography (HPSEC) coupled with multi angle laser light scattering (MALLS) and refractive index detector (RID) with the refractive index increment (dn/dc). Results showed that HPSEC chromatograms and molecular weight distributions of polysaccharides in L. barbarum collected from different regions of China were similar. Furthermore, the average contents of each polysaccharide fraction (peaks 1, 2, and 3) in crude polysaccharides of L. barbarum collected from Ningxia were similar with those of Inner Mongolia, Xinjiang, and Gansu, respectively. However, significant difference was found between polysaccharides in L. barbarum collected from Ningxia and Qinghai. Moreover, the average amounts of total polysaccharide fractions (peaks 1, 2, and 3) in the raw material of L. barbarum collected from Ningxia were significantly higher than that of Qinghai. These results may contribute to the rational usage of L. barbarum produced in China, and are beneficial for the improvement of their quality control. Results suggested that HPSEC-MALLS-RID with the dn/dc method could be used as a routine method for the quality evaluation of polysaccharides from natural resources and their products.


Assuntos
Lycium/química , Polissacarídeos/química , Água/química , China , Cromatografia em Gel/métodos , Frutas/química , Peso Molecular , Refratometria
16.
Carbohydr Polym ; 134: 12-9, 2015 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-26428094

RESUMO

Water-soluble polysaccharides from 51 batches of fruits of L. barbarum (wolfberry) in China were investigated and compared using saccharide mapping, partial acid hydrolysis, single and composite enzymatic digestion, followed by polysaccharide analysis by using carbohydrate gel electrophoresis (PACE) analysis and high performance thin layer chromatography (HPTLC) analysis, respectively. Results showed that multiple PACE and HPTLC fingerprints of partial acid and enzymatic hydrolysates of polysaccharides from L. barbarum in China were similar, respectively. In addition, results indicated that ß-1,3-glucosidic, α-1,4-galactosiduronic and α-1,5-arabinosidic linkages existed in polysaccharides from L. barbarum collected in China, and the similarity of polysaccharides in L. barbarum collected from different regions of China was pretty high, which are helpful for the improvement of the performance of polysaccharides from L. barbarum in functional/health foods area. Furthermore, polysaccharides from Panax notoginseng, Angelica sinensis, and Astragalus membranaceus var. mongholicus were successfully distinguished from those of L. barbarum based on their PACE fingerprints. These results were beneficial to improve the quality control of polysaccharides from L. barabrum and their products, which suggested that saccharide mapping based on PACE and HPTLC analysis could be a routine approach for quality control of polysaccharides.


Assuntos
Lycium/química , Polissacarídeos/química , Cromatografia em Camada Delgada , Eletroforese em Gel de Poliacrilamida , Hidrólise , Polissacarídeos/isolamento & purificação
17.
Artigo em Inglês | MEDLINE | ID: mdl-24055753

RESUMO

High-speed counter-current chromatography (HSCCC) was successfully applied for the first time to isolate and purify four cis-trans isomers of coumaroylspermidine analogs from Safflower. HSCCC separation was achieved with a two-phase solvent system composed of chloroform-methanol-water (1:1:1, v/v/v) with the upper phase as the mobile phase. In a single run, a total of 1.3mg of N(1), N(5), N(10)-(E)-tri-p-coumaroylspermidine (EEE), 4.4mg of N(1)(E)-N(5)-(Z)-N(10)-(E)-tri-p-coumaroylspermidine (EZE), 7.2mg of N(1)(Z)-N(5)-(Z)-N(10)-(E)-tri-p-coumaroylspermidine (ZZE), and 11.5mg of N(1),N(5),N(10)-(Z)-tri-p-coumaroylspermidine (ZZZ) were obtained from 100mg of crude sample. High Performance Liquid Chromatography (HPLC) analysis showed that the purities of these four components are 95.5%, 98.1%, 97.5% and 96.2%, respectively. The chemical structures were identified by ESI-MS, (1)H NMR and (13)C NMR.


Assuntos
Carthamus tinctorius/química , Ácidos Cumáricos/isolamento & purificação , Distribuição Contracorrente/métodos , Espermidina/análogos & derivados , Espermidina/isolamento & purificação , Ácidos Cumáricos/análise , Ácidos Cumáricos/química , Isomerismo , Modelos Moleculares , Extratos Vegetais/química , Espermidina/análise , Espermidina/química
18.
Zhong Yao Cai ; 36(3): 377-81, 2013 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-24010316

RESUMO

OBJECTIVE: To establish the HPLC fingerprint of Gymnadenia conopsea from different regions of Qinghai-Tibet Plateau. METHODS: 12 batches of Gymnadenia conopsea were measured by the RP-HPLC, and their fingerprints were obtained. Chromatographic condition: The sample was separated on column of Kromasil C18 (4.6 mm x 250 mm,5 microm) and gradiently eluted with a mixture of methanol and water (containing 0.04% phosphoric acid). The flow rate was 0.7 mL/min. The detection wavelength was set at 222 nm and the column temperature was 30 degrees C. RESULTS: The HPLC fingerprint of Gymnadenia conopsea was set up and 13 common peaks were selected,the results of method validation met technical requirement of fingerprint,the similarity of 12 batches Gymnadenia conopsea was 0.904 - 0.989. Principal components analysis and clustering analysis were used in the identification of the samples. CONCLUSION: The method is simple, practicable and reliable, which can be used for the quality control of Gymnadenia conopsea.


Assuntos
Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas/química , Orchidaceae/química , China , Medicamentos de Ervas Chinesas/análise , Controle de Qualidade
19.
Zhongguo Zhong Yao Za Zhi ; 38(7): 1004-7, 2013 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-23847946

RESUMO

A new compound (1), together with ten known compounds (2-11), have been isolated from the branch of Litsea greenmaniana by using various chromatographic techniques. Their structures were identified by spectroscopic data analysis as N-trans-3, 4-methylenecinnamoyl-3-methoxytyramine (1), N-trans-feruloyltyramine (2), N-cis-feruloyltyramine (3), (+)-sesamin (4), (+)-pinoresinol(5), cinnamophilin (6), dihydrodehydrodiconiferyl alcohol (7), benzoic acid (8), 4-hydroxy ethylbenzoate (9), p-hydroxybenzaldehyde(10), and 4-hydroxy-3-methoxy-benzyl alcohol (11). Compound 1 was a new compound, and compounds 2-11 were obtained from this plant for the first time.


Assuntos
Medicamentos de Ervas Chinesas/química , Litsea/química , Espectrometria de Massas , Estrutura Molecular
20.
J Pharm Biomed Anal ; 49(4): 1097-100, 2009 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-19268518

RESUMO

In order to evaluate the differences and similarities between the liposoluble constituents in Cynomorium songaricum populations, stem liposoluble constituents in five populations of C. songaricum collected from three different geographic regions and four different hosts were obtained by solvent extraction and analyzed by GC-MS. Cluster analysis of the percentage composition of 80 compounds showed differences in chemical composition which were related to the geographic origin rather than the host. Hexadecanoic acid was the most abundant compound in the essential oils of C. songaricum from hosts Nitraria sibirica and Nitraria tanguticum. Whereas (Z)-9-octadecenoic acid was accumulated in the oils of C. songaricum from Zygophyllum xanthoxylum and Peganum harmala. Four of the five populations had characteristic components, which were specific to each population.


Assuntos
Cynomorium/química , Lipídeos/química , Caules de Planta/química , Análise por Conglomerados , Cromatografia Gasosa-Espectrometria de Massas , Indicadores e Reagentes , Óleos Voláteis/análise , Solventes
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