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1.
Front Pediatr ; 10: 957790, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36340736

RESUMO

Objective: This study aimed to compare the effects of various trocar placements in robot-assisted and laparoscopic pyeloplasty involving children diagnosed with obstruction of the ureteropelvic junction (OUPJ). Methods: We retrospectively collected the data on 74 patients under 14 years of age who had been diagnosed with OUPJ; these patients underwent either robot-assisted or laparoscopic pyeloplasty in our hospital between January 2015 and November 2021. There were four groups, as follows: •Laparoscopic multiport pyeloplasty (LMPY),•Laparoscopic single-port pyeloplasty (LSPY),•Robotic-assisted multiport pyeloplasty (RMPY),•Robotic-assisted single-port-plus-one pyeloplasty (RSPY).Patients' characteristics as well as their perioperative and follow-up data were collected and evaluated. Results: There was no significant difference in the data regarding patients' characteristics. These data included the grade of hydronephrosis according to the Society of Fetal Urology (SFU grade), anterior and posterior diameter of the renal pelvis and ureter (APDRPU), and the differential degree of renal function (DRF) at following time points: preoperative, postoperative, and comparison of preoperative and postoperative. There was no difference among these groups. During surgery, the time of trocar placement, urethroplasty time, and total operative time in the robotic groups (RMPY and RSPY) were longer than those in the laparoscopic groups (LMPY and LSPY). However, the ratio of the urethroplasty time and full operative time (UT/WT) in the robotic groups (RMPY and RSPY) was lower than that in the laparoscopic groups (LMPY and LSPY) (P = 0.0075). Also, the volume of blood loss was lower in the robotic groups (RMPY and RSPY) than that in the laparoscopic groups (LMPY and LSPY), although there was no statistical difference (P = 0.11). There were, however, significant differences in hospitalization days (P < 0.0001) and parents' cosmetic satisfaction scores (P < 0.001). There were no differences in fasting time, the length of time that a ureteral catheter remained in place, or the number of postoperative complications. Conclusion: Our study shows that both robotic multiple-port and single-port-plus-one approaches are comparable, with laparoscopic multiple-port and single-port approaches equally effective in resolving OUPJ in children. Robotic and single-port-plus-one approaches may be associated with some advantages in hospitalization time and cosmetic outcomes; therefore, these approaches may be useful in urologic surgery that requires precise suturing, especially in pediatric patients.

2.
Medicine (Baltimore) ; 101(43): e31325, 2022 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-36316942

RESUMO

The selection criterion for liver resection (LR) in intermediate-stage (IM) hepatocellular carcinoma (HCC) is still controversial. This study aims to compare LR and transarterial chemoembolization (TACE) in the range of predicted death risk The multivariable Cox regression model (MVR) was estimated to predict mortality at 5 year. The cutoff values were determined by a 2-piece-wise linear regression model, decision curve analysis with MVR model, and hazard ratio curve for treatment plotted against the predicted mortality. 825 IM-hepatocellular carcinoma (IM-HCC) with hepatitis B cirrhosis were included for analysis (TACE, n = 622; LR, n = 203). The 5-year overall survival (OS) rate of LR patients was higher than the TACE group (52.8% vs 20.8%; P < .0001). The line of LR and TACE were crossing with predicted death risk at 100% (P for interaction = .008). The benefit of LR versus TACE decreased progressively as predicted death risk > 0.55 (95%CI: 0.45, 0.62). When predicted death risk over 0.7, decision curve analysis suggested that LR and TACE did not increase net benefit. Patients were then divided into 4 subgroups by the cutoff values (<0.45, 0.45≥/<0.62, 0.62≥/<0.7, ≥0.7). The stratified analysis of treatment in different subgroups, hazard ratios were 0.39 (95%CI: 0.27, 0.56), 0.36 (95%CI: 0.23, 0.56), 0.51 (95%CI: 0.27, 0.98), and 0.46 (95%CI: 0.27, 0.80), respectively. LR reached the maximal relative utility in the interval of 0.45 to 0.62, and both LR and TACE did not increase net benefit at the 5-year death risk over 0.7.


Assuntos
Carcinoma Hepatocelular , Quimioembolização Terapêutica , Hepatite B , Neoplasias Hepáticas , Humanos , Carcinoma Hepatocelular/cirurgia , Carcinoma Hepatocelular/patologia , Neoplasias Hepáticas/cirurgia , Neoplasias Hepáticas/patologia , Estudos de Coortes , Resultado do Tratamento , Hepatectomia , Hepatite B/terapia , Cirrose Hepática/cirurgia , Estudos Retrospectivos
3.
Front Med (Lausanne) ; 9: 859318, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36213627

RESUMO

Background: Patients with severe acute kidney injury (AKI) may require renal replacement therapy (RRT), such as hemodialysis and peritoneal dialysis. Neutrophil gelatinase-associated lipocalin (NGAL) is a sensitive indicator for early diagnosis and recognition of AKI; however, its predictive value of AKI-associated need for RRT needs further evaluation. Methods: Following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis guidelines, relevant articles were systematically searched and selected from seven databases. The random effects model was applied to evaluate the predictive performance of NGAL for AKI requiring RRT. The Newcastle-Ottawa Scale (NOS) was used to assess the quality of each included study. Results: A total of 18 studies including 1,787 patients with AKI and having an average NOS score of 7.67 were included in the meta-analysis. For plasma/serum NGAL, the pooled sensitivity and specificity with corresponding 95% confidence interval (CI) were 0.75 (95% CI: 0.68-0.81) and 0.76 (95% CI: 0.70-0.81), respectively. The pooled positive likelihood ratio (PLR) was 2.9 (95% CI: 2.1-4.1), and the pooled negative likelihood ratio (NLR) was 0.34 (95% CI: 0.25-0.46). Subsequently, the pooled diagnostic odds ratio (DOR) was 9 (95% CI: 5-16) using a random effects model, and the area under the curve (AUC) of summary receiver operating characteristic to summarize predictive accuracy was 0.82 (95% CI: 0.79-0.85). For urine NGAL, the pooled sensitivity, specificity, PLR, NLR, DOR, and AUC values were 0.78 (95% CI: 0.61-0.90), 0.77 (95% CI: 0.65-0.85), 3.4 (95% CI: 2.4-4.8), 0.28 (95% CI: 0.15-0.52), 12 (95% CI: 6-24), and 0.84 (95% CI: 0.80-0.87), respectively. Conclusion: Plasma/serum and urine NGAL levels performed comparably well in predicting AKI requiring RRT. Our findings suggested that NGAL is an effective predictive biomarker for the AKI-associated need for RRT. Nevertheless, more pieces of high-quality evidence and future trials with larger sample sizes are needed for further improvement of patient outcomes. Systematic review registration: [https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022346595], identifier [CRD42022346595].

4.
Shanghai Kou Qiang Yi Xue ; 31(3): 290-294, 2022 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-36204959

RESUMO

PURPOSE: To explore the influence of different adduction methods of maxillary incisor in adolescents on the shape of alveolar bone. METHODS: Three hundred and twenty-eight maxillary incisors were collected from 82 patients with jaw protrusion deformity treated in Union Hospital of Fujian Medical University from June 2018 to June 2019. 135 incisors were included in the oblique adduction group, and 193 incisors were included in the root control adduction group. All patients took cone-beam CT(CBCT) to measure the adduction of the maxillary incisor and the changes in alveolar bone height, thickness, and bone density. Statistical analysis was performed with SPSS 18.0 software package. RESULTS: The length of the maxillary incisor to the mid-palatine vertical surface(IE-MP), the long axis of the maxillary incisor and the posterior inferior angle of the palatal plane (LAI-PP) in oblique adduction group were significantly greater than root control adduction group(P<0.05). The length of the maxillary incisor from the apex of the maxillary incisor to the mid-palatine vertical surface(RA-MP) in the oblique adduction group was significantly smaller than the root control adduction group (P<0.05). Postoperative L1, L2, L3, P1, T1 of the oblique adduction group were significantly lower than preoperative values(P<0.05), and postoperative P2, P3, T2, and T3 were significantly higher than preoperative value(P<0.05). Postoperative L1, L2, and L3 of the root control adduction group were significantly higher than those before operation (P<0.05), and postoperative P1, P2, P3, T1, T2, and T3 were significantly lower than those before operation(P<0.05). The changes of L2, L3, P3, T3 of the oblique adduction group were significantly higher than the control root adduction group(P<0.05), and the changes of L1, P1, P2, T1, T2 of the oblique adduction group were all significantly lower than the control root adduction group(P<0.05). There was no significant difference in bone mineral density of the upper and lower jaws between the two groups before and 6 months after operation(P>0.05). CONCLUSIONS: When adolescents' maxillary incisors are retracted in a wide range, the labial apical area, the palatal alveolar ridge area of the oblique adduction group, and the palatal root neck of the root control adduction group are all high-risk areas for alveolar bone resorption.


Assuntos
Perda do Osso Alveolar , Incisivo , Adolescente , Processo Alveolar/diagnóstico por imagem , Tomografia Computadorizada de Feixe Cônico , Humanos , Incisivo/diagnóstico por imagem , Maxila/diagnóstico por imagem , Raiz Dentária
5.
Anal Chem ; 94(41): 14290-14298, 2022 10 18.
Artigo em Inglês | MEDLINE | ID: mdl-36198125

RESUMO

We propose to replace the traditional time-frequency domain filtering with feature domain filtering to realize an innovation of filtering algorithm. A feature domain transform filter (FDTF) is composed of the feature domain transform layer based on principal component analysis (PCA) algorithm, the feature domain information extractor based on deep learning and the time domain transform layer. It is established to filter out the noise with the same frequency and phase as the signal and is verified on methane gas. Although FDTF is established based on the simulated data set, the filtering effects of the simulation test set and the experimental data set show that the proposed FDTF outperforms other widely used time-frequency filtering algorithms. The FDTF-assisted methane sensor has good linearity at different concentrations of methane gas. With the FDTF enhancement, the optimized methane sensor performs excellent precision and stability in real-time measurements and achieves the minimum detectable column density of 2.50 ppm·m. This is undoubtedly a successful attempt to move the signal to a new domain for parsing and separation.


Assuntos
Algoritmos , Metano , Simulação por Computador , Análise de Componente Principal , Processamento de Sinais Assistido por Computador , Razão Sinal-Ruído
6.
Adv Mater ; : e2206961, 2022 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-36281802

RESUMO

Interfaces formed by correlated oxides offer a critical avenue for discovering emergent phenomena and quantum states. However, the fabrication of oxide interfaces with variable crystallographic orientations and strain states integrated along a film plane is extremely challenging by conventional layer-by-layer stacking or self-assembling. Here, the creation of morphotropic grain boundaries (GBs) in laterally interconnected cobaltite homostructures is reported. Single-crystalline substrates and suspended ultrathin freestanding membranes provide independent templates for coherent epitaxy and constraint on the growth orientation, resulting in seamless and atomically sharp GBs. Electronic states and magnetic behavior in hybrid structures are laterally modulated and isolated by GBs, enabling artificially engineered functionalities in the planar matrix. This work offers a simple and scalable method for fabricating unprecedented innovative interfaces through controlled synthesis routes as well as providing a platform for exploring potential applications in neuromorphics, solid-state batteries, and catalysis.

7.
Adv Mater ; : e2208221, 2022 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-36300813

RESUMO

Interfacial magnetism stimulates the discovery of giant magnetoresistance (MR) and spin-orbital coupling across the heterointerfaces, facilitating the intimate correlation between spin transport and complex magnetic structures. Over decades, functional heterointerfaces composed of nitrides have seldom been explored due to the difficulty in synthesizing high-quality nitride films with correct compositions. Here, the fabrication of single-crystalline ferromagnetic Fe3 N thin films with precisely controlled thicknesses is reported. As film thickness decreases, the magnetization dramatically deteriorates, and the electronic state changes from metallic to insulating. Strikingly, the high-temperature ferromagnetism is maintained in a Fe3 N layer with a thickness down to 2 u.c. (≈8 Å). The MR exhibits a strong in-plane anisotropy; meanwhile, the anomalous Hall resistivity reverses its sign when the Fe3 N layer thickness exceeds 5 u.c. Furthermore, a sizable exchange bias is observed at the interfaces between a ferromagnetic Fe3 N and an antiferromagnetic CrN. The exchange bias field and saturation moment strongly depend on the controllable bending curvature using the cylinder diameter engineering technique, implying the tunable magnetic states under lattice deformation. This work provides a guideline for exploring functional nitride films and applying their interfacial phenomena for innovative perspectives toward practical applications.

8.
Biochem Pharmacol ; 206: 115329, 2022 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-36309080

RESUMO

Therapeutic targeting of the nuclear enzyme poly (ADP-ribose) polymerase 1 (PARP1) with PARP inhibitors (PARPis) in patients with a homologous recombination (HR)- deficient phenotype based on the mechanism of synthetic lethality has been shown tremendous success in cancer therapy. With the clinical use of various PARPis, emerging evidence has shown that some PARPis offer hope for breakthroughs in triple-negative breast cancer (TNBC) therapy, regardless of HR status. However, similar to other conventional cytotoxic drugs, PARPis are also subject to the intractable problem of drug resistance. Notably, acquired resistance to PARPis caused by point mutations in the PARP1 protein is hard to overcome with current strategies. To explore modalities to overcome resistance and identify patients who are most likely to benefit from PARP1-targeted therapy, we developed a proteolysis-targeted chimaera (PROTAC) to degrade mutant PARP1 in TNBC. Here, we investigated a PARP1 PROTAC termed "NN3″, which triggered ubiquitination and proteasome-mediated degradation of PARP1. Moreover, NN3 degraded PARP1 with resistance-related mutations. Interestingly, compared with other reported PARP1 degraders, NN3 exhibited a unique antitumor mechanism in p53-positive breast cancer cells that effectively promoted ferroptosis by downregulating the SLC7A11 pathway. Furthermore, NN3 showed potent activity and low toxicity in vivo. In conclusion, we propose PROTAC-mediated degradation of PARP1 as a novel strategy against mutation-related PARPi resistance and a paradigm for targeting breast cancer with functional p53 via ferroptosis induction.

9.
Neurosci Bull ; 2022 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-36260252

RESUMO

Defensive behaviors induced by innate fear or Pavlovian fear conditioning are crucial for animals to avoid threats and ensure survival. The zona incerta (ZI) has been demonstrated to play important roles in fear learning and fear memory, as well as modulating auditory-induced innate defensive behavior. However, whether the neuronal subtypes in the ZI and specific circuits can mediate the innate fear response is largely unknown. Here, we found that somatostatin (SST)-positive neurons in the rostral ZI of mice were activated by a visual innate fear stimulus. Optogenetic inhibition of SST-positive neurons in the rostral ZI resulted in reduced flight responses to an overhead looming stimulus. Optogenetic activation of SST-positive neurons in the rostral ZI induced fear-like defensive behavior including increased immobility and bradycardia. In addition, we demonstrated that manipulation of the GABAergic projections from SST-positive neurons in the rostral ZI to the downstream nucleus reuniens (Re) mediated fear-like defensive behavior. Retrograde trans-synaptic tracing also revealed looming stimulus-activated neurons in the superior colliculus (SC) that projected to the Re-projecting SST-positive neurons in the rostral ZI (SC-ZIrSST-Re pathway). Together, our study elucidates the function of SST-positive neurons in the rostral ZI and the SC-ZIrSST-Re tri-synaptic circuit in mediating the innate fear response.

10.
Chem Sci ; 13(35): 10342-10348, 2022 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-36277647

RESUMO

Subcellular organelle targeted transport is of great significance for accurately delivering drugs to active sites for better pharmacological effects, but there are still a lot of challenges due to transport problems. In addition, the killing effect of one kind of drug on cells is limited. Therefore, it is necessary to develop a multifunctional nanoplatform that can co-deliver synergistic therapeutic agents. Here, we prepare a simple amphiphilic nanocarrier (LC) with rapid endosomal escape ability for nucleus-selective delivery of hydrophilic active protein deoxyribonuclease I (DNase I) and hydrophobic anticancer drug doxorubicin (DOX). LC has been applied to effectively encapsulate DNase I just by simply mixing their aqueous solutions together. In addition, DOX modified with adamantane groups via a redox-responsive linker is incorporated into the architecture of DNase I nanoformulations through host-guest interaction. This multi-component nanoplatform can quickly escape from the endolysosomes into the cytoplasm and make DNase I and DOX highly accumulate in the nucleus and consequently induce strong synergistic anticancer efficacy both in vitro and in vivo. This work illustrates a new platform for codelivery of proteins and drugs that target subcellular compartments for functions.

12.
Nanomaterials (Basel) ; 12(20)2022 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-36296902

RESUMO

We have demonstrated the method of threshold voltage (VT) adjustment by controlling Ge content in the SiGe p-channel of N1 complementary field-effect transistor (CFET) for conquering the work function metal (WFM) filling issue on highly scaled MOSFET. Single WFM shared gate N1 CFET was used to study and emphasize the VT tunability of the proposed Ge content method. The result reveals that the Ge mole fraction influences VTP of 5 mV/Ge%, and a close result can also be obtained from the energy band configuration of Si1-xGex. Additionally, the single WFM shared gate N1 CFET inverter with VT adjusted by the Ge content method presents a well-designed voltage transfer curve, and its inverter transient response is also presented. Furthermore, the designed CFET inverter is used to construct a well-behaved 6T-SRAM with a large SNM of ~120 mV at VDD of 0.5 V.

13.
Sensors (Basel) ; 22(20)2022 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-36298108

RESUMO

The printed circuit board (PCB) is the core control unit of electromechanical equipment. In order to determine the influence of the coupling vibration caused by vehicle-road interaction on the PCB reliability of roadside electromechanical equipment, first, the dynamic load of the vehicle tire is solved by establishing the dynamic model of a vehicle road. Then, the acceleration response data generated by road vibration are obtained by solving the road finite element model. Finally, the power density spectrum of the acceleration response is taken as input excitation, and the deformation response of the PCB under vehicle-road coupling vibration is analyzed. The experimental results show that when the vehicle is driving close to the roadside, the vibration caused by vehicle-road coupling will lead to a large deformation of the PCB, and the deformation value reaches 0.170 mm, which can cause structural damage to the PCB. This shows that the vehicle-road coupling vibration can affect the reliability of the roadside electromechanical equipment; thus, the optimal design of the PCB layout is created. After optimization, the first-order modal frequency of the PCB is increase by 5.4%, which reduces the risk of the components breaking away from the PCB substrate.


Assuntos
Condução de Veículo , Reprodutibilidade dos Testes , Aceleração , Vibração
14.
Sci Adv ; 8(43): eabq3981, 2022 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-36306366

RESUMO

Emergent phenomena at heterointerfaces are directly associated with the bonding geometry of adjacent layers. Effective control of accessible parameters, such as the bond length and bonding angles, offers an elegant method to tailor competing energies of the electronic and magnetic ground states. In this study, we construct unit-thick syntactic layers of cobaltites within a strongly tilted octahedral matrix via atomically precise synthesis. The octahedral tilt patterns of adjacent layers propagate into cobaltites, leading to a continuation of octahedral tilting while maintaining substantial misfit tensile strain. These effects induce severe rumpling within an atomic plane of neighboring layers, further triggering the electronic reconstruction between the splitting orbitals. First-principles calculations reveal that the cobalt ions transit to a higher spin state level upon octahedral tilting, resulting in robust ferromagnetism in ultrathin cobaltites. This work demonstrates a design methodology for fine-tuning the lattice and spin degrees of freedom in correlated quantum heterostructures by exploiting epitaxial geometric engineering.

15.
Front Cell Dev Biol ; 10: 933042, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36268514

RESUMO

Cellular unjamming is the collective fluidization of cell motion and has been linked to many biological processes, including development, wound repair, and tumor growth. In tumor growth, the uncontrolled proliferation of cancer cells in a confined space generates mechanical compressive stress. However, because multiple cellular and molecular mechanisms may be operating simultaneously, the role of compressive stress in unjamming transitions during cancer progression remains unknown. Here, we investigate which mechanism dominates in a dense, mechanically stressed monolayer. We find that long-term mechanical compression triggers cell arrest in benign epithelial cells and enhances cancer cell migration in transitions correlated with cell shape, leading us to examine the contributions of cell-cell adhesion and substrate traction in unjamming transitions. We show that cadherin-mediated cell-cell adhesion regulates differential cellular responses to compressive stress and is an important driver of unjamming in stressed monolayers. Importantly, compressive stress does not induce the epithelial-mesenchymal transition in unjammed cells. Furthermore, traction force microscopy reveals the attenuation of traction stresses in compressed cells within the bulk monolayer regardless of cell type and motility. As traction within the bulk monolayer decreases with compressive pressure, cancer cells at the leading edge of the cell layer exhibit sustained traction under compression. Together, strengthened intercellular adhesion and attenuation of traction forces within the bulk cell sheet under compression lead to fluidization of the cell layer and may impact collective cell motion in tumor development and breast cancer progression.

16.
J Cancer ; 13(12): 3318-3325, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36186899

RESUMO

Background: Transarterial chemoembolization (TACE) is the standard first-line therapy for intermediate-stage hepatocellular carcinoma (HCC). However, no latent-classing indices, concerning repeat conventional TACE or switching to another treatment, have been incorporated into the guidelines. Methods: The unsupervised latent class modeling was applied to identify subphenotypes using the clinical and medical imaging data of 1517 HCC patients after the first TACE from four hospitals (derivation cohort: 597 cases; validation cohort: 920 cases); modeling was conducted independently in each cohort. We then explored the relationship of subphenotypes with clinical outcomes in both cohorts and response to treatment strategies after the first TACE in the derivation cohort. Results: Independent latent class models suggested that a three-class model was optimal for both cohorts. In both cohorts, we identified a TACE-refractory subphenotype (Phenotype 1: PS score 1, stage progress, more intrahepatic lesions, and new intrahepatic lesions), TACE-responsive subphenotype (Phenotype 3: PS score 0, No intrahepatic lesions and new intrahepatic lesions), compared to TACE-intermediate subphenotype (Phenotype 2). Compared to Phenotype 1 or 2, patients in Phenotype 3 had significantly lower 3-month or 3-year mortality (all P<0.001). In the derivation cohort, the effects of treatment strategy (surgery/ablation vs. repeat TACE vs. stop TACE) differed significantly in phenotype 2 but not in phenotype 3 (P=0.721 for interaction). Conclusions: Latent class models identified three subphenotypes for HCC after the first TACE treatment. Differences were significant in clinical outcome and response to treatment strategy after the first TACE among three subphenotypes.

17.
Int J Biol Macromol ; 222(Pt B): 1700-1708, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36179870

RESUMO

The lacquer seed oil has received extensive attention in the food industry due to its health function, such as regulating blood lipids. But its by-product, lacquer seed meal, is often used as a low-value-added product such as animal feed. Lacquer seed meal contains about 20 % protein, which has amphiphilic properties, and there is limited attention to its emulsifying properties. In this study, the impact of heat treatment on the emulsifying properties of lacquer seed protein isolate (LSPI) was investigated. The EAI and ESI of the 120 °C heated LSPI increased by 77.1 % and 55.2 %, respectively. The emulsions prepared using heat-modified LSPI (120 °C) further showed lower hydroperoxide, TBARS and protein carbonyl contents (only 61.3 %, 61.0 % and 58.6 % of control) after storage. This result indicates that heat-treated LSPI retarded lipid and protein oxidation in LSPI-stabilized emulsions during storage. Changes in protein structure showed that increasing heating temperature resulted in the depolymerization of tertiary structure, higher surface hydrophobicity and lower contents of α-helix of LSPI. These changes in protein structure made the heated LSPIs have better emulsifying properties. Therefore, these findings developed a new use of LSPI and greatly enhanced the potential of LSPI as a natural emulsifier in the food industry.


Assuntos
Temperatura Alta , Laca , Animais , Emulsões/química , Emulsificantes/química , Sementes/química , Proteínas/análise
18.
Nat Prod Res ; : 1-7, 2022 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-36093561

RESUMO

Two new compounds, compounds 1 and 2, were obtained from the culture of a marine-derived fungus Talaromyces sp. MCCC 3A01752, together with 13 known compounds (3-15). Their structures were elucidated based on detailed analysis of NMR, HRESIMS, ECD spectra and OR value. Compound 1 exhibited antibacterial potential against Staphylococcus aureus with a MIC value of 100 µM and cytotoxic activity against gastric cancer cell line MKN1 with a IC50 value of 78.0 µM.

19.
Annu Int Conf IEEE Eng Med Biol Soc ; 2022: 4839-4842, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-36086106

RESUMO

In image-guided surgery, endoscope tracking and surgical scene reconstruction are critical, yet equally challenging tasks. We present a hybrid visual odometry and reconstruction framework for stereo endoscopy that leverages unsupervised learning-based and traditional optical flow methods to enable concurrent endoscope tracking and dense scene reconstruction. More specifically, to reconstruct texture-less tissue surfaces, we use an unsupervised learning-based optical flow method to estimate dense depth maps from stereo images. Robust 3D landmarks are selected from the dense depth maps and tracked via the Kanade-Lucas-Tomasi tracking algorithm. The hybrid visual odometry also benefits from traditional visual odometry modules, such as keyframe insertion and local bundle adjustment. We evaluate the proposed framework on endoscopic video sequences openly available via the SCARED dataset against both ground truth data, as well as two other state-of-the-art methods - ORB-SLAM2 and Endo-depth. Our proposed method achieved comparable results in terms of both RMS Absolute Trajectory Error and Cloud-to-Mesh RMS Error, suggesting its potential to enable accurate endoscope tracking and scene reconstruction.


Assuntos
Fluxo Óptico , Algoritmos , Endoscópios , Endoscopia Gastrointestinal , Imageamento Tridimensional/métodos
20.
Biomed Pharmacother ; 153: 113407, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36076533

RESUMO

Hypertension has become one of the important diseases harmful to human health. In China, Qingda granule (QDG) has been used to treat hypertension for decades. Previous studies by our team have shown that oxidative stress may be one of the pathways through which QDG inhibits hypertension-induced organs injury. However, the specific molecular mechanism of its anti-hypotension and renal oxidative stress response were unclearly. This study investigated QDG's potential protective mechanism against hypertension-induced renal injury. Mice were infused with Angiotensin Ⅱ (Ang Ⅱ, 500 ng/kg/min) or equivalent saline solution (Control) and administered oral QDG (1.145 g/kg/day) or saline for four weeks. QDG treatment mitigated the elevated blood pressure and reduced renal pathological changes induced by Ang Ⅱ. As per the RNA sequencing results, QDG affects oxidative stress signaling. In agreement with these findings, QDG significantly attenuated the Ang Ⅱ-induced increase in Nitrogen oxides 1 (NOX1) and reactive oxygen species and the decrease in superoxide dismutase in renal tissue. Additionally, QDG significantly inhibited Interleukin 6 (IL-6), Tumor necrosis factor α (TNF-α), and Interleukin 1ß (IL-1ß) expression in renal tissues and blocked the phosphorylation of P65 (NF-κB subunit) and IκB. These results were confirmed in vitro. Overall, QDG reduced Ang Ⅱ-induced elevated blood pressure and renal injury by inhibiting oxidative stress and inflammation caused by NOX1 and NF-κB pathways. The results of this study provide an experimental basis for the clinical application of QDG, and to open up a new direction for the clinical treatment of hypertension.


Assuntos
Angiotensina II , Hipertensão , Angiotensina II/efeitos adversos , Angiotensina II/toxicidade , Animais , Medicamentos de Ervas Chinesas , Hipertensão/induzido quimicamente , Hipertensão/tratamento farmacológico , Hipertensão/metabolismo , Inflamação/metabolismo , Rim/patologia , Camundongos , NF-kappa B/metabolismo , Óxidos de Nitrogênio/metabolismo , Óxidos de Nitrogênio/uso terapêutico , Estresse Oxidativo/efeitos dos fármacos
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