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1.
Mol Cancer Res ; 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-32051231

RESUMO

Hepatocellular carcinomas (HCCs) are adapted to survive extreme genomic stress conditions imposed by hyperactive DNA replication and genotoxic drug treatment. The underlying mechanisms remain unclear, but may involve intensified DNA damage response/repair programs. Here, we investigate a new role of nucleostemin (NS) in allowing HCC to survive its own malignancy, as NS was previously shown to promote liver regeneration via a damage repair mechanism. We first established that a higher NS transcript level correlates with high HCC grades and poor prognostic signatures, and is an independent predictor of shorter overall and progression-free survival specifically for HCC and kidney cancer but not for others. Immunostaining confirmed that NS is most abundantly expressed in high-grade and metastatic HCCs. Genome-wide analyses revealed that NS is co-enriched with MYC target and homologous recombination (HR) repair genes in human HCC samples and functionally intersects with those involved in replication stress response and HR repair in yeasts. In support, NS-high HCCs are more reliant on the replicative/oxidative stress response pathways, whereas NS-low HCCs depend more on the mTOR pathway. Perturbation studies showed NS function in protecting human HCC cells from replication- and drug-induced DNA damage. Notably, NS depletion in HCC cells increases the amounts of physical DNA damage and cytosolic dsDNA, leading to a reactive increase of cytokines and PD-L1. This study shows that NS provides an essential mechanism for HCC to adapt to high genomic stress for oncogenic maintenance and propagation. NS deficiency sensitizes HCC cells to chemotherapy but also triggers tumor immune responses. Implications: Hepatocellular carcinoma (HCC) employs a novel, nucleostemin mediated adaptive mechanism to survive high genomic stress conditions, a deficiency of which sensitizes HCC cells to chemotherapy but also triggers tumor immune responses.

2.
Artigo em Inglês | MEDLINE | ID: mdl-32019305

RESUMO

The heterogeneous Fenton-like process is regarded as a promising approach to produce reactive oxygen species for water purification and environmental remediation. Here, we report a simple and rational strategy for the design of an efficient catalyst by reducing the dimensionality instead of changing the composition or structure. Based on theoretical and experimental evidence, considerable active sites were exposed on the low-dimensional Ti3C2Tx monolayer surface and showed outstanding reactivity toward peroxymonosulfate activation, which was mainly because of the superior compatibility between the highest occupied molecular orbital of catalysts and lowest unoccupied molecular orbital of Oxone. Stimulated emission depletion super-resolution microscopy innovatively provided visual insights into the spatiotemporal heterogeneous activation process and revealed that the unilaminar Ti3C2Tx nanosheet exhibited preferable reaction dynamics relative to its inert bulk counterpart, with an aqueous 2,4-dichlorophenoxyacetic acid degradation rate ∼376 times higher than that when using bulk Ti3C2Tx as the activator.

3.
Ann Transplant ; 25: e920224, 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32029699

RESUMO

BACKGROUND ABO-incompatible (ABOi) living-donor kidney transplantation (KTx) is well established in developed countries, but not yet in China. MATERIAL AND METHODS We developed individualized preconditioning protocols for ABOi KTx based on initial ABO antibody titers. After propensity score matching of ABOi with ABO-compatible (ABOc) KTx, post-transplant outcomes were compared. RESULTS Between September 2014 and June 2018, 48 ABOi living-donor KTx candidates received individualized preconditioning, and all underwent subsequent KTx (median initial ABO titers: 16 for IgM and 16 for IgG). Thirty-one recipients (64.6%) were preconditioned with rituximab (median dose: 200 mg, range: 100-500 mg). Among 37 patients (77.1%) who received pre-transplant antibody removal, the median number of sessions of antibody removal required to achieve ABOi KTx was 2 (range: 1-5), which was conducted between days -10 and -1. Eleven ABOi recipients (22.9%) were preconditioned with oral immunosuppressants alone. Hyperacute rejection led to the loss of 2 grafts in the ABOi group. After a median follow-up of 27.6 months (ABOi group) and 29.8 months (ABOc group), there were no significant differences in graft/recipient survival, rejection, and infection. There were marginally higher rates of severe thrombocytopenia (<50×109/L) (P=0.073) and delayed wound healing (P=0.096) in ABOi recipients. CONCLUSIONS Our individualized preconditioning protocol evolved as our experience grew, and the short-term clinical outcomes of ABOi KTx did not differ from those of matched ABOc patients. ABOi KTx may be a major step forward in expanding the kidney living-donor pool in China.

4.
Sci Total Environ ; 717: 137205, 2020 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-32062283

RESUMO

Soluble microbial products (SMPs), as precursors of disinfection byproducts (DBPs) in water treatment, are composed of polysaccharides, humic acid, proteins and DNA, and have caused widespread concerned. Pyrimidine bases (cytosine and thymine) are significant nitrogenous constituents of DNA, which could pose an adverse impact on water quality during chlorination. This study focused on the correlation between relative reactivity, formation of DBPs and combined toxicity in the chlorination of a binary pyrimidine base mixture. The relative reactivities of cytosine and thymine were quite different at a low disinfectant concentration; cytosine reacted more actively with chlorine than thymine did, at the chlorine/total pyrimidine bases molar ratio = 10. The chlorination of binary pyrimidine bases can produce both carbonous DBPs (C-DBPs) and nitrogenous DBPs (N-DBPs). In particular, the total yields of trichloromethane (TCM) and trichloronitromethane (TCNM) were lower than the additive yields of monadic cytosine and monadic thymine ("monadic" refers to "separate"), whereas the total yields of haloacetic acids (HAAs) and haloacetonitriles (HANs) were promoted evidently. High reactivity of cytosine with chlorine, greater potential of cytosine to produce specific DBPs and the alkylation of transformation products of thymine may synthetically account for the diversity in total DBPs yields, especially the increased formation of HAAs and HANs. In our toxicity trial, even though the antagonistic effect predominated at fa > 0.4 (fa refers to the affected fraction), the synergism at low concentration levels could enhance the combined toxicity by promoting the yields of N-DBPs.

5.
Nanoscale ; 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32016263

RESUMO

Recently, the discovery and development of lead-free perovskite quantum dots (QDs) that are eco-friendly and stable has become an active research area in low-cost lighting and display fields. However, the low photoluminescence quantum yield (PLQY) caused by the residual surface states of such QDs severely hinders their practical applications and commercialization. In this work, a strategy of employing water-induced nanocomposites was proposed to improve the PLQY of cesium bismuth halide (Cs3Bi2X9) QDs, and a substantial enhancement by ∼130% (from 20.2% to 46.4%) was achieved by an optimized water treatment of Cs3Bi2Br9 QDs. A detailed analysis indicated that Cs3Bi2Br9/BiOBr nanocomposites, in which the Cs3Bi2Br9 QD core was encapsulated into a BiOBr matrix, can effectively suppress the surface defects of QDs, resulting in a longer PL lifetime and a larger exciton binding energy compared with the pristine sample. Finally, the Cs3Bi2Br9/BiOBr nanocomposites were used as the color-converting phosphors for down-conversion white light-emitting devices, which show a good operation stability in ambient air, significantly better than the reference device constructed with conventional lead-halide perovskites. We believe that the method used here provides an effective strategy to improve the fluorescence efficiency of lead-free perovskite QDs, which will create opportunities for their applications in lighting and displays.

6.
Biomed Pharmacother ; 125: 109880, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-32004974

RESUMO

BACKGROUND: MicroRNA-155-5p (miR-155-5p) has been reported to play an oncogenic role in different human malignancies; however, its role in Wilms tumor (WT) remains unclear. METHODS: Differentially expressed miRNAs (DE-miRNAs) and mRNAs (DEGs) in WT blood and tissues were identified by using miRNA microarray and RNA-sequencing. Bioinformatics prediction and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were used to predict the potential functions of DE-miRNAs. DE-miRNAs and DEGs in WT obtained from Gene Expression Omnibus (GEO) and Therapeutically Applicable Research to Generate Effective Treatments (TARGET) were identified by using the "edgeR" package. RT-qPCR was used to explore miR-155-5p and IGF2 expression and their clinical significance in WT specimens. A rhabdoid cell line (G401) and Ewing sarcoma cell line (SK-NEP-1) were used. Immunohistochemical staining, western blotting and dual-luciferase reporter assays were performed to study the mechanisms involved. The CCK-8, flow cytometry, wound healing and transwell assays were performed to identify the effects of miR-155-5p and IGF2 knockdown on cell proliferation, apoptosis, migration and invasion, respectively. RESULTS: MiR-155-5p was downregulated in both blood and tissues from WT patients who did not receive chemotherapy before surgery but was upregulated in tissues from WT patients who had received chemotherapy before surgery. IGF2, PI3K, AKT and mTOR were found to be upregulated in WT tissues. Additionally, miR-155-5p and IGF2 were significantly correlated with TNM stage and lymphatic metastasis in WT patients. Molecular mechanism exploration indicated that IGF2 was downregulated by miR-155-5p via direct binding to its 3' untranslated region in cell lines. Furthermore, IGF2, PI3K, AKT and mTOR expression was inversely correlated with miR-155-5p expression, and PI3K, AKT and mTOR expression was positively correlated with IGF2 expression in cell culture. Functional studies demonstrated that miR-155-5p upregulation and IGF2 knockdown suppressed cell proliferation, migration and invasion and induced cell apoptosis. Moreover, the tumor-suppressing effects of miR-155-5p in cells were abrogated by miR-155-5p inhibitor treatment. CONCLUSIONS: Taken together, these findings suggest that miR-155-5p functions as a tumor suppressor in WT through inactivating the PI3K/AKT/mTOR signaling pathway by directly targeting IGF2. Thus, miR-155-5p might be a novel therapeutic target for WT.

7.
Orphanet J Rare Dis ; 15(1): 13, 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31937334

RESUMO

BACKGROUND: Myasthenia gravis is a rare autoimmune neuromuscular disorder. The disorder requires long-term use of expensive medication to control clinical symptoms. This study analyzed the change in trends of total medical expenses and out-of-pocket expenses for patients with myasthenia gravis and explored the factors influencing them. METHODS: In this retrospective study, data were derived from a survey of medical service utilization for patients insured by the Urban Basic Medical Insurance in China from 2013 to 2015. The cost data of 3347 patients with myasthenia gravis were included in this study. The baseline characteristics and medical expenses for patients with myasthenia gravis were analyzed using a descriptive method. The difference and influencing factors of the out-of-pocket ratio were analyzed from both outpatient and inpatient expenses by using the quantile regression method. RESULTS: The total expenses reimbursed by the Urban Basic Medicine Insurance for all patients with myasthenia gravis fell progressively from 73.1 to 58.7% during the study period. Patients' out-of-pocket expenses increased gradually, of which expenses within the scope of Basic Medicine Insurance increased from 14.7 to 22.6% and expenses outside of the Basic Medicine Insurance scope increased from 12.6 to 18.7%. Moreover, the panel quantile results showed a positive correlation between the year of receiving treatment and the out-of-pocket ratio. In addition to the 25th quantile of the out-of-pocket ratio among outpatients with myasthenia gravis, there were significant differences in medical insurance and medical institution among all the other quantiles. Significant regional differences were found in all quantiles of the out-of-pocket ratio, except for the 75th quantile among inpatients. Lastly, age had a negative effect on inpatients with myasthenia gravis across all quantiles, but not on outpatients. CONCLUSIONS: From 2013 to 2015, patients with myasthenia gravis's out-of-pocket expenses increased progressively. Moreover, the individual out-of-pocket ratio was affected by the year, medical insurance, medical institution, region, and age. The current medical insurance policy for the general public has a low ability to cater for patients with myasthenia gravis.

8.
J Coll Physicians Surg Pak ; 30(1): 41-45, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31931931

RESUMO

OBJECTIVE: To assess the correlation between urodynamic study findings and urotherapy treatment response in children with urotherapy-resistant overactive bladder (OAB). STUDY DESIGN: A cross-sectional study. PLACE AND DURATION OF STUDY: Department of Pediatric Urology, Children's Hospital of Chongqing Medical University, Chongqing, China, from January 2016 to March 2018. METHODOLOGY: The study included 232 children presented with urotherapy-resistant OAB defined as (completing threemonth standard urotherapy with sustained OAB symptoms. After excluding urinary infection and neurogenic, anatomical abnormalities, urodynamic study (UDS) was proceeded to record DO (detrusor overactivity) and maximum CBC (cystometry bladder capacity), pelvic floor awareness was guided during UDS. Following UDS, standard urotherapy and pelvic floor training was continued for another month and treatment effect was evaluated. RESULTS: At the end of one-month; of urotherapy following UDS, significant response (SR) was observed in 57.3% (133/232) children. As 28.9% (67/232) children showed partial response (PR), the other 13.8% (32/232) children showed no response (NR). The results showed that patients with normal bladder function or DO respond equally well to urotherapy. The percentage of significant response (SR) was 68.9% (93/135) and 60.0% (27/45) (p=0.148), which was significantly better than children with decreased CBC (DCBC) (SR: 33.3%, 10/30) or DO+DCBC (SR: 13.6%, 3/22, p=0.000). CONCLUSION: Urodynamic verification of DO is not a prerequisite for urotherapy treatment response. Decreased cystometry bladder capacity (DCBC) found in UDS may suggest a poorer treatment response to urotherapy than patients with normal CBC.

9.
Matern Child Nutr ; : e12924, 2020 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-31970860

RESUMO

To assess the vitamin D status in healthy 6-month-old infants, as well as vitamin D supplementation and feeding patterns in Guangzhou, China, serum 25-hydroxyvitamin D (25OHD) concentrations of 202 infants were measured at birth (cord blood) and at 6 months of age in Guangzhou, China. Questionnaires acquiring demographic characteristics, maternal and infantile vitamin D supplementation during pregnancy and first 6 months after birth, and feeding patterns during the first 6 months were completed by participating mothers. Physical examinations and blood sampling were carried out among infants at 6 months of age. The majority of infants (93.6%) were supplemented with vitamin D during the first 6 months of life on a voluntary basis. The M ± SD of cord serum 25OHD concentration was 46.2 ± 16.4 nmol/L, whereas the M ± SD of 25OHD concentration at 6 months was 82.9 ± 24.9 nmol/L. Serum 25OHD concentrations <30 nmol/L were seen in 34 (16.8%) infants at birth but only one (0.5%) at 6 months. Only 11 (5.4%) infants had concentrations >75 nmol/L at birth, whereas the majority of infants (n = 131, 64.9%) had concentrations >75 nmol/L at 6 months. The main predictors of 25OHD levels at 6 months included season, vitamin D supplementation, parental education level, and feeding patterns. To conclude, serum 25OHD concentrations were low at birth in a southern Chinese population, and infantile supplementation is an effective way to improve 25OHD status. Exclusively breastfed infants might need greater vitamin D supplementation, and individualized vitamin D supplementation plans might be needed.

10.
Chemosphere ; 246: 125840, 2020 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-31927386

RESUMO

Sludge water was recycled using an ultrafiltration (UF) system. In order to control membrane fouling, three typical Fe(II)-activated peroxymonosulfate (PMS) processes: Fe(II)/PMS-UF (FPUF), 1/4Fe(II) × 4/PMS-UF (F4PUF) (adding Fe(II) in small increments four times), and Fe(II)/thiosulfate/PMS-UF (FTPUF) (adding Fe(II) after complexing with thiosulfate), were employed as UF pretreatments. Their mitigating effects of UF membrane fouling caused by sludge water are systematically discussed and compared. The results showed that FTPUF system had the best membrane fouling control effect. The F4PUF system was more suitable for long-term operation than FPUF due to its lower irreversible resistance. The pretreatments can effectively remove contaminants from sludge water through the dual effects of coagulation and oxidation. Specifically, coagulation removed most of the particles and macromolecular organic matter. Oxidation effectively decomposed fluorescent and UV-absorbing organic components, and reduced bacterial proliferation on the membrane surface. Concentrations of 2-methylisoborneol and geosmin in FPUF permeate were dramatically increased, which was mainly due to the rupture of algal cells in sludge water. Both adhesion force date and extended Derjaguin-Landau-Verwey-Overbeek theory indicated that the pretreatments significantly reduced interactions between the membrane-organic colloid and cake layer-organic colloid. In contrast, the stronger membrane-organic colloid interaction was a major contributor to membrane fouling. The mitigation of irreversible fouling was mainly attributed to the fact that oxidation enhanced the hydrophilicity of the organic colloids, thereby reducing the Lewis acid-base interaction energy. The results demonstrated the potential application of different Fe(II)-activated PMS processes as pretreatments for membrane fouling control during sludge water treatment.

11.
Artigo em Inglês | MEDLINE | ID: mdl-31959566

RESUMO

BACKGROUND AND OBJECTIVE: To systematically evaluate the efficacy, safety, and treatment-related outcomes between percutaneous radiofrequency ablation (PtRFA) and partial hepatectomy (PH) for small primary liver cancer meeting the Milan criteria. METHODS: A systematic review and meta-analysis were conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) statement. A priori protocol was registered in the PROSPERO database, and a literature search of all relevant studies published on PubMed, Cochrane library, Web of Science database, and Science Direct until July 2019 was performed. Only randomized controlled trials published in English were eligible for inclusion. RESULTS: Of the 385 identified articles, only five randomized controlled trials involving 761 adult patients with small liver cancer were included in the final analysis. The 1-year, 2-year, 3-year, 4-year, 5-year overall survival and disease-free survival between PtRFA and PH did not reach significant difference (P-value>0.05). The long-term recurrence rate was higher and the recurrence-free survival was lower in patients treated with PtRFA compared with those treated with PH (P-value<0.05), but short-term (1-year) comparisons did not reach statistical significance. With regard to treatment-related complications, serious adverse event risks, analgesics needs, hospital stay and costs, PtRFA had significant superiority compared with PH. CONCLUSIONS: The overall treatment effects of PtRFA are comparable to PH for small liver cancer. Though patients with PtRFA have a higher long-term recurrence rate, these patients share less complications, few serious adverse event risks, less analgesics needs, shorter hospital stay, and lower costs. PtRFA may be recommended as the preferred treatment of solitary liver tumors<3cm in diameter.

12.
Medicine (Baltimore) ; 99(1): e18571, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31895801

RESUMO

BACKGROUND: There is a growing literature on the significance of systemic immune-inflammation index in hepatocellular carcinoma. However, the results were inconsistent due to the small sample size and different study endpoints. Therefore, the purpose of this study was to further systematically and comprehensively verify the prognostic role of the SII in HCC. METHODS: Several databases were searched systematically, and relevant papers were selected. The main outcome measure was overall survival (OS); the secondary outcome measure was a composite of time to recurrence (TTR), progression-free survival (PFS), and recurrence-free survival (RFS). RESULTS: Ten published retrospective studies involving 2796 HCC patients were included. The results revealed that elevated pre-treatment SII was related to lower OS (HR:1.54, P < .001) and earlier TTR (HR:1.77, P < .001). CONCLUSIONS: Elevated SII is a poor prognostic factor for patients with hepatocellular carcinoma. The clinical application of SII is encouraged to evaluate the progress of hepatocellular carcinoma.


Assuntos
Carcinoma Hepatocelular/diagnóstico , Carcinoma Hepatocelular/mortalidade , Neoplasias Hepáticas/diagnóstico , Neoplasias Hepáticas/mortalidade , Índice de Gravidade de Doença , Idoso , Feminino , Humanos , Inflamação , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia/diagnóstico , Recidiva Local de Neoplasia/mortalidade , Valor Preditivo dos Testes , Prognóstico , Intervalo Livre de Progressão , Estudos Retrospectivos
13.
Environ Sci Technol ; 54(3): 1484-1492, 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-31927953

RESUMO

Dissolved organic nitrogen (DON) represents a unique challenge in prechlorinated raw water distribution systems (PRWDSs) because of its contribution to the formation of harmful nitrogen-disinfection byproducts, influence upon biogeochemical processes, and unclear molecular characteristics. Here, Fourier transform ion cyclotron resonance mass spectrometry in combination with high-throughput sequencing was applied to elucidate the molecular changes of DON and biofilm microbial communities in a PRWDS in Yixing, China. Our study revealed that dynamic characteristics of DON are significantly correlated with the biofilm. The accumulation of refractory lignin-like compounds and CnHmOpN1 contributes to the higher recalcitrance molecular characteristics of DON in the effluent associated with Alphaproteobacteria, Planctomycetes, and Bacteroidetes. Additionally, with the help of prechlorination, the biofilm may change the DON characteristics and lead to higher oxygenation, higher m/z, and lower saturation during transportation. Despite the promotion of CnHmOpN1 and CnHmOpN3 at the early stage, we suggest that appropriate concentration of chlorine can add to the front end of raw water distribution pipes. Prechlorination may control the nitrification process and stabilize the rapid growth of diversity and concentration of low molecular weight DON, especially the refractory CnHmOpN1 in the effluent, which may help to improve treatment efficiency of drinking water treatment plants.

14.
Sci Total Environ ; 709: 136097, 2020 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-31905568

RESUMO

Quantifying the spatial association between ecological indicators (e.g., chlorophyll-a) and environmental parameters is crucial for explaining the ecological status in coastal ecosystems. Although global and local regression models have been widely used to estimate spatial relationships in marine environmental processes, spatial anisotropy caused by strong coastal-inland environmental gradients has not been investigated. This is very likely to result in incomprehensive characterization of the coastal ecological status. To better quantify the spatially anisotropic nonstationary relationship in coastal environments, a spatial proximity neural network (SPNN) was proposed in this paper to address the nonlinear effects of spatial anisotropy. A directional geographically neural network weighted regression (DGNNWR) model was accordingly developed by combining a geographically neural network weighted regression (GNNWR) with SPNN to incorporate anisotropic impacts into spatial nonstationarity. Modeling of chlorophyll-a in Zhejiang coastal areas of China in the spring over 2015-2017 was conducted to examine its performance. The results demonstrated that DGNNWR achieved a better fitting accuracy and a more adequate prediction ability than ordinary linear regression (OLR), geographically weighted regression (GWR), GNNWR, and anisotropic-based GWR models. Notably, compared to the best comparison model, the fitting error indicators were declined for more than 30% and the fitted R2 was considerably increased from 0.83 to 0.92 using our proposed DGNNWR. The spatial mapping of parameter estimates confirmed that DGNNWR successfully handled the anisotropic nonstationarity in coastal environments and quantified the main driven parameters of Chl-a. Based on the spatially refined relationship between Chl-a and environmental parameters, we further characterized the spatial and temporal distributions of Chl-a in Zhejiang coastal areas in the spring of 2015-2017, and then investigated the impacts of riverine discharges and ocean currents on the spatiotemporal variations of Chl-a. The findings are crucial to formulate appropriate mitigation strategies for eutrophication and are meaningful for the management of coastal ecosystems.

15.
Pediatr Res ; 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31896126

RESUMO

BACKGROUND: Renal fibrosis occurs largely through epithelial-mesenchymal transition (EMT). This study explored the beneficial effects of a human umbilical cord mesenchymal stem cell-loaded decellularized kidney scaffold (ucMSC-DKS) on renal fibrosis in a rodent model of post-transplantation renal failure, and the underlying mechanism. METHODS: Rat-derived DKSs were examined after preparation, and then recellularized with human ucMSCs to prepare cell-loaded patches. A rat model of renal failure was established after subtotal nephrectomy (STN). The cell patches were transplanted to remnant kidneys. Changes in renal function, histology, EMT, and proteins related to the transforming growth factor-ß (TGF-ß)/Smad signaling pathway in the remnant kidneys were examined 8 weeks after surgery, compared with non-cell patch controls. RESULTS: The DKSs were acellular and porous, with rich cytokine and major extracellular matrix components. The ucMSCs were distributed uniformly in the DKSs. Renal function was improved, renal fibrosis and EMT were reduced, and the TGF-ß/Smad signaling pathway was inhibited compared with controls at 8 weeks after ucMSC-DKS patch transplantation. CONCLUSIONS: The ucMSC-DKS restores renal function and reduces fibrosis by reducing EMT via the TGF-ß/Smad signaling pathway in rats that have undergone STN. It provides an alternative for renal fibrosis treatment.

16.
Acta Otolaryngol ; 140(1): 8-13, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31782327

RESUMO

Background: Whether the abnormal caloric test (C-test) affects recurrence rate in horizontal semicircular canal benign paroxysmal positional vertigo (HSC-BPPV) with residual dizziness (RD) is not clear.Objectives: 1) Analyze the association of the cycles of canalith repositioning procedure (CRP), C-test and RD after CRP and 2) determine which affects the recurrence rate in idiopathic HSC-BPPV.Materials and methods: Eighty-four patients with HSC-BPPV (canal type) were included in this work. The cycles of CRP, C-test, the RD after CRP and HSC-BPPV recurrence rate were recorded. Depending on the times of CRP and patients who presented dizziness after treatment, patients were divided into four groups, the relationship between abnormal C-test and RD was analyzed. The outcomes of recurrence rate were compared between groups, respectively.Results: (1) The abnormal C-test prevalence among the HSC-BPPV patients with RD was 36% while in no RD group was 14.7%. The difference was statistically significant (p = .045). (2) The recurrence rate was 11.8% in no RD group but in RD group the rate was higher (32%, p = .039). When patients combined with abnormal C-test, the recurrence rate was significantly higher (77.8% vs. 20%) (p = .033).Conclusions: A weak correlation between RD and abnormal C-test is noted. Presence of RD and abnormal C-test in patients with HSC-BPPV predicts a higher recurrence rate.

17.
Plant Physiol ; 182(1): 167-184, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31378719

RESUMO

Histone deacetylase (HDAC) proteins participate in diverse and tissue-specific developmental processes by forming various corepressor complexes with different regulatory subunits. An important HDAC machinery hub, the Histone Deacetylase Complex1 (HDC1) protein, participates in multiple protein-protein interactions and regulates organ size in plants. However, the mechanistic basis for this regulation remains unclear. Here, we identified a cucumber (Cucumis sativus) short-fruit mutant (sf2) with a phenotype that includes repressed cell proliferation. SF2 encodes an HDC1 homolog, and its expression is enriched in meristematic tissues, consistent with a role in regulating cell proliferation through the HDAC complex. A weak sf2 allele impairs HDAC targeting to chromatin, resulting in elevated levels of histone acetylation. Genome-wide mapping revealed that SF2 directly targets and promotes histone deacetylation associated with key genes involved in multiple phytohormone pathways and cell cycle regulation, by either directly repressing or activating their expression. We further show that SF2 controls fruit cell proliferation through targeting the biosynthesis and metabolism of cytokinin and polyamines. Our findings reveal a complex regulatory network of fruit cell proliferation mediated by HDC1 and elucidate patterns of HDC1-mediated regulation of gene expression.

18.
Sci Total Environ ; 700: 134520, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31669914

RESUMO

Microplastics (MPs) have attracted worldwide attention as the emerging persistent pollutants. Since they have been detected in raw water and the treated water of drinking water treatment plants (DWTPs), there was an urgent need to explore the properties and fates of microplastics in DWTPs. The characteristics of the effluent MPs from each treatment unit in an advanced drinking water treatment plant (ADWTP) were studied, and the relationship between the variations of MPs and the removal performances of treatment processes was also explored. Overall, both the coagulation combined with sedimentation and the granular activated carbon (GAC) filtration performed well in removing microplastics. The former had a removal efficiency of about 40.5-54.5%, mainly for fibres' removal, and the presence of GAC filtration reduced the microplastic abundance by about 56.8-60.9%, mainly for small-sized MPs. It was worthy of attention that a larger amount of polyacrylamide (PAM) was detected in the effluent of the sedimentation compared to raw water, which was caused by the usage of coagulant containing PAM. Specially, the number of 1-5 µm MPs in the effluent of ozonation tank was increased by 2.8-16.0%, resulting in a negative removal efficiency in ozonation. The removals of microplastics were depended primarily on their physical properties (size and shape).


Assuntos
Água Potável/química , Monitoramento Ambiental , Poluentes Químicos da Água/análise , Purificação da Água/métodos
19.
J Hazard Mater ; 382: 121064, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31499370

RESUMO

The development of non-cobalt-based heterogeneous catalysts with efficient catalytic activity, good stability and nontoxicity is very important for the application of peroxymonosulfate-based advanced oxidation processes (AOPs) in water treatment. In this work, with two dimensional MXene as the catalyst substrate, a novel α-Fe2O3/MXene (FM) nanocomposite was fabricated through a facile solvothermal method. Systematic characterization demonstrated that the MXene substrate could facilitate the size reduction and good dispersion of α-Fe2O3 nanoparticles. The FM nanocomposite achieved high efficiency and stability towards activating peroxymonosulfate (PMS) to produce free radicals for the degradation of salicylic acid (SA) in aqueous solution. The operating parameters, including catalyst dosage, PMS dosage, SA concentration and initial pH value, were evaluated and analysed. The co-existence of sulfate radicals (SO4-) and hydroxyl radicals (OH) was confirmed using electron paramagnetic resonance spectroscopy and radical scavenger tests, while SO4-was identified as the main reactive species in the FM/PMS catalytic system. The possible mechanisms for the electron transfer and radical generation during the process of PMS activation by the FM nanocomposite are further investigated using XPS and in situ Raman analysis. The results provide an avenue for rationally constructing and developing alternative catalysts for the treatment of organics in wastewater.

20.
Plant Physiol ; 182(2): 908-918, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31843803

RESUMO

Flowering time plays a crucial role in the geographical adaptation of most crops during domestication. Cucumber (Cucumis sativus) is a major vegetable crop worldwide. From its tropical origin on the southern Asian continent, cucumber has spread over a wide latitudinal cline, but the molecular mechanisms underlying this latitudinal adaptation and the expansion of domesticated cucumber are largely unclear. Here, we report the cloning of two flowering time loci from two distinct cucumber populations and show that two large deletions upstream from FLOWERING LOCUS T (FT) are associated with higher expression of FT and earlier flowering. We determined that the two large deletions are pervasive and occurred independently in Eurasian and East-Asian populations. Nucleotide diversity analysis further revealed that the FT locus region of the cucumber genome contains a signature for a selective sweep during domestication. Our results suggest that large genetic structural variations upstream from FT were selected for and have been important in the geographic spread of cucumber from its tropical origin to higher latitudes.

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