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1.
J Biochem ; 2021 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-34117764

RESUMO

OBJECTIVE: To explore the interaction between miR-133a-3p and CDCA8 in esophageal cancer (EC) and their effect on malignant behavior of EC cells. METHODS: Differential miRNAs and mRNAs were obtained from The Cancer Genome Atlas (TCGA) database. Quantitative real-time PCR (qRT-PCR) was used to detect the expression levels of miR-133a-3p and CDCA8 mRNA in EC cells. Western blot was used to detect the expression of CDCA8 protein. CCK-8, flow cytometry, and Transwell assays were conducted to detect cell proliferation, cell cycle and apoptosis, as well as migration and invasion, respectively. The targeting relationship between miR-133a-3p and CDCA8 was verified by dual-luciferase reporter gene assay. RESULTS: In EC, miR-133a-3p expression was evidently low and CDCA8 expression was prominently high. MiR-133a-3p down-regulated CDCA8 expression. A range of cell function experiments revealed that CDCA8 promoted the proliferation, migration and invasion of EC cells, reduced cell cycle arrest in G0/G1 phase and inhibited cell apoptosis, while miR-133a-3p could reverse the above effects by regulating CDCA8. CONCLUSION: MiR-133a-3p is a crucial tumor suppressor miRNA in EC, playing a tumor suppressor role by targeting CDCA8.

2.
Sci Rep ; 11(1): 10839, 2021 May 25.
Artigo em Inglês | MEDLINE | ID: mdl-34035389

RESUMO

Early detection and appropriate medical treatment are of great use for ear disease. However, a new diagnostic strategy is necessary for the absence of experts and relatively low diagnostic accuracy, in which deep learning plays an important role. This paper puts forward a mechanic learning model which uses abundant otoscope image data gained in clinical cases to achieve an automatic diagnosis of ear diseases in real time. A total of 20,542 endoscopic images were employed to train nine common deep convolution neural networks. According to the characteristics of the eardrum and external auditory canal, eight kinds of ear diseases were classified, involving the majority of ear diseases, such as normal, Cholestestoma of the middle ear, Chronic suppurative otitis media, External auditory cana bleeding, Impacted cerumen, Otomycosis external, Secretory otitis media, Tympanic membrane calcification. After we evaluate these optimization schemes, two best performance models are selected to combine the ensemble classifiers with real-time automatic classification. Based on accuracy and training time, we choose a transferring learning model based on DensNet-BC169 and DensNet-BC1615, getting a result that each model has obvious improvement by using these two ensemble classifiers, and has an average accuracy of 95.59%. Considering the dependence of classifier performance on data size in transfer learning, we evaluate the high accuracy of the current model that can be attributed to large databases. Current studies are unparalleled regarding disease diversity and diagnostic precision. The real-time classifier trains the data under different acquisition conditions, which is suitable for real cases. According to this study, in the clinical case, the deep learning model is of great use in the early detection and remedy of ear diseases.

5.
Food Funct ; 2021 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-34019057

RESUMO

Correction for 'Yulangsan polysaccharide improves redox homeostasis and immune impairment in d-galactose-induced mimetic aging' by Van Minh Doan et al., Food Funct., 2015, 6, 1712-1718, DOI: 10.1039/C5FO00238A.

6.
Cell ; 184(12): 3318-3332.e17, 2021 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-34038702

RESUMO

Long-term subcellular intravital imaging in mammals is vital to study diverse intercellular behaviors and organelle functions during native physiological processes. However, optical heterogeneity, tissue opacity, and phototoxicity pose great challenges. Here, we propose a computational imaging framework, termed digital adaptive optics scanning light-field mutual iterative tomography (DAOSLIMIT), featuring high-speed, high-resolution 3D imaging, tiled wavefront correction, and low phototoxicity with a compact system. By tomographic imaging of the entire volume simultaneously, we obtained volumetric imaging across 225 × 225 × 16 µm3, with a resolution of up to 220 nm laterally and 400 nm axially, at the millisecond scale, over hundreds of thousands of time points. To establish the capabilities, we investigated large-scale cell migration and neural activities in different species and observed various subcellular dynamics in mammals during neutrophil migration and tumor cell circulation.

7.
Nat Commun ; 12(1): 2404, 2021 04 23.
Artigo em Inglês | MEDLINE | ID: mdl-33893309

RESUMO

Geothermal environments, such as hot springs and hydrothermal vents, are hotspots for carbon cycling and contain many poorly described microbial taxa. Here, we reconstructed 15 archaeal metagenome-assembled genomes (MAGs) from terrestrial hot spring sediments in China and deep-sea hydrothermal vent sediments in Guaymas Basin, Gulf of California. Phylogenetic analyses of these MAGs indicate that they form a distinct group within the TACK superphylum, and thus we propose their classification as a new phylum, 'Brockarchaeota', named after Thomas Brock for his seminal research in hot springs. Based on the MAG sequence information, we infer that some Brockarchaeota are uniquely capable of mediating non-methanogenic anaerobic methylotrophy, via the tetrahydrofolate methyl branch of the Wood-Ljungdahl pathway and reductive glycine pathway. The hydrothermal vent genotypes appear to be obligate fermenters of plant-derived polysaccharides that rely mostly on substrate-level phosphorylation, as they seem to lack most respiratory complexes. In contrast, hot spring lineages have alternate pathways to increase their ATP yield, including anaerobic methylotrophy of methanol and trimethylamine, and potentially use geothermally derived mercury, arsenic, or hydrogen. Their broad distribution and their apparent anaerobic metabolic versatility indicate that Brockarchaeota may occupy previously overlooked roles in anaerobic carbon cycling.


Assuntos
Archaea/genética , Ciclo do Carbono/genética , Genoma Arqueal/genética , Metagenoma/genética , Filogenia , Archaea/classificação , Archaea/metabolismo , Carbono/metabolismo , China , Geografia , Sedimentos Geológicos/microbiologia , Fontes Termais/microbiologia , Fontes Hidrotermais/microbiologia , Metano/metabolismo , RNA Ribossômico 16S/genética , Especificidade da Espécie
8.
Zhongguo Zhen Jiu ; 41(3): 303-6, 2021 Mar 12.
Artigo em Chinês | MEDLINE | ID: mdl-33798314

RESUMO

OBJECTIVE: To observe the imaging features of focus of knee joint tendon in patients with knee osteoarthritis (KOA) by musculoskeletal ultrasound (MSUS) technique. METHODS: One hundred KOA patients and 100 healthy subjects were included. All the KOA patients were palpated by the sequence of foot yangming meridian, foot shaoyang meridian, foot taiyang meridian and foot three yin-meridians, and the focus of knee tendon and its frequency were recorded by MSUS technique. The top-5 focus of knee tendon of KOA patients and the corresponding focus of knee tendon of healthy subjects were selected as the observation sites to compare the differences of thickness of ligament and tendon soft tissue in the extension and flexion positions of the knee joint. RESULTS: The top-5 focus of knee tendon of KOA patients were located in medial inferior patella, medial tibial condyle, inferior patella, Zusanlici and Hedingci. The thickness of ligaments and tendons in extension and flexion positions in KOA patients were thicker than that in healthy subjects (P<0.05). CONCLUSION: The focus of knee joint tendon in KOA patients shows significantly thickened musculoskeletal imaging features.


Assuntos
Osteoartrite do Joelho , Voluntários Saudáveis , Humanos , Articulação do Joelho/diagnóstico por imagem , Osteoartrite do Joelho/diagnóstico por imagem , Tendões/diagnóstico por imagem , Ultrassonografia
9.
Dalton Trans ; 50(14): 4904-4913, 2021 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-33877187

RESUMO

Different aromatic polycarboxylic acids are employed as auxiliary ligands to give rise to structural diversities in Co(ii)-tpt (tpt = 2,4,6-tris(4-pyridyl)-1,3,5-triazine) frameworks. By introducing various secondary aromatic polycarboxylate anions, namely, biphenyl-3,4',5-tricarboxylic acid (H3bpt), 1,3,5-benzenetricarboxylic acid (H3btc) and 2,6-dimethyl pyridine-3,5-dicarboxylic acid (H2dmdcpy) into the Co(ii)-tpt system (tpt = 2,4,6-tris(4-pyridyl)-1,3,5-triazine), four new complexes [Co3(tpt)2(Hbpt)3]·0.5DMDP (1) (DMDP = N,N' = dimethylpropyleneurea), [Co3(btc)2(tpt)(H2O)3]·3H2O (2), [Co2(btc)(tpt)2Cl]·DMDP·1.5H2O (3) and [Co(tpt)(dmdcpy)]·H2O (4) were obtained. Complexes 1 and 2 reveal amazing 3D networks in which the polycarboxylate ligands and Co(ii) ions connect with each other to form regular 3D porous frameworks with 1D cylindrical channels partitioned by virtue of the tpt ligands. Complexes 3 and 4 exhibit unusual 3D frameworks constructed from the Co-polycarboxylate layers pillared by tpt ligands. In addition, compound 1 was chosen as a precursor to prepare Co, N-codoped porous carbon materials (denoted as CoNC) as an eletrocatalyst for oxygen reduction reactions. In particular, the effect of different nitrogen sources on the electrocatalytic performance of MOF derived carbon materials was investigated. We found that although different nitrogen-containing ligands have a certain effect on the electrocatalytic performance of the synthesized CoMOF derived carbon materials, the additional nitrogen source has a significant effect on it. CoNC-A derived from compound 1 exhibits greater limiting current density than that of a Pt/C catalyst, while CoNC-B derived from a mixture of compound 1 and dicyandiamide shows almost identical onset potential but remarkably more positive half-wave potential as well as higher limiting current density as compared to commercial Pt/C catalysts.

10.
Biomed Res Int ; 2021: 6546170, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33778068

RESUMO

Pitaya (Hylocereus genus) is a popular plant with exotic and nutritious fruit, which has widespread uses as a source of nutrients and raw materials in the pharmaceutical industry. However, the potential of pitaya peel as a natural source of bioactive compounds has not yet fully been explored. Recent advances in metabolomics have paved the way for understanding and evaluating the presence of diverse sets of metabolites in different plant parts. This study is aimed at exploring the diversity of primary and secondary metabolites in two commercial varieties of pitaya, i.e., green pitaya (Hylocereus undatus) and red pitaya (Hylocereus polyrhizus). A total of 433 metabolites were identified using a widely targeted metabolomic approach and classified into nine known diverse classes of metabolites, including flavonoids, amino acids and its derivatives, alkaloids, tannins, phenolic acids, organic acids, nucleotides and derivatives, lipids, and lignans. Red pitaya peel and pulp showed relatively high accumulation of metabolites viz. alkaloids, amino acids and its derivatives, and lipids. Differential metabolite landscape of pitaya fruit indicated the presence of key bioactive compounds, i.e., L-tyrosine, L-valine, DL-norvaline, tryptophan, γ-linolenic acid, and isorhamnetin 3-O-neohesperidoside. The findings in this study provide new insight into the broad spectrum of bioactive compounds of red and green pitaya, emphasizing the valorization of the biowaste pitaya peel as raw material for the pharmaceutical and food industries.


Assuntos
Cactaceae , Flavonoides/análise , Indústria Alimentícia , Frutas , Extratos Vegetais/análise , Cactaceae/química , Cactaceae/metabolismo , Frutas/química , Frutas/metabolismo , Metabolômica
12.
Int Immunopharmacol ; 92: 107208, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33444919

RESUMO

Hepatic stellate cell (HSC) activation is a crucial event in the progress of liver fibrosis. In this study, the target of helenalin was firstly predicted by bioinformatics analysis, and then the prediction was verified by various experiments. HSC-T6 cells were activated by interleukin-1 beta (IL-1ß) and then treated with helenalin. Moreover, HSC-T6 cells were transfected with miR-200a mimic or inhibitor, and the effect of helenalin on the miR-200a-mediated PI3K/Akt and NF-κB signaling pathways was investigated. The bioinformatics analysis indicated that miR-200a might regulate the PI3K/Akt pathway, NF-κB activation, Bcl-2 family and Caspases, ultimately affecting cell survival and apoptosis. Interestingly, the molecular docking demonstrated that the target of helenalin might be miR-200a-mediated the PI3K/Akt and NF-κB pathways. Moreover, the experiments showed that helenalin administration led to the inactivation of HSC-T6 cells, as evidenced by the inhibition of cell proliferation, α-SMA expression and collagen production. The mechanism studies showed that helenalin reduced collagen accumulation by restoring the balance of MMPs/TIMPs. Moreover, helenalin markedly suppressed HSC activation by inhibiting the PI3K/Akt pathway and alleviated inflammatory response by blocking the NF-κB signal transduction. Further study indicated that helenalin up-regulated miR-200a expression, thus leading to the inhibition of the PI3K/Akt and NF-κB signaling pathways. In conclusion, helenalin inhibits HSC activation via inhibiting the miR-200a-mediated PI3K/Akt and NF-κB pathways, and it may be developed as a potential medicine for the treatment of liver fibrosis.

13.
Rev Sci Instrum ; 92(1): 013105, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33514246

RESUMO

The second-order photon correlation function g2(τ) is of great importance in quantum optics. g2(τ) is typically measured with the Hanbury Brown and Twiss (HBT) interferometer, which employs a pair of single-photon detectors and a dual-channel time acquisition module. Here, we demonstrate a new method to measure and extract g2(τ) with a standard single-photon avalanche photodiode (dead-time = 22 ns) and a single-channel time acquisition module. This is realized by shifting the coincidence counts of interest to a time window not affected by the dead-time and after-pulse of the detection system using a fiber-based delay line. The new scheme is verified by measuring g2(τ) from a single colloidal nanocrystal. Photon antibunching is unambiguously observed and agrees well with the result measured using the standard HBT setup. Our scheme simplifies the higher-order correlation technique and might be favored in cost-sensitive circumstances.

16.
Toxicol Lett ; 339: 88-96, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33423876

RESUMO

The roots of Averrhoa carambola L. (Oxalidaceae) have a long history of medical use in traditional Chinese medicine for treating diabetes and diabetic nephropathy. 2-dodecyl-6-methoxycyclohexa-2, 5-diene-1, 4-dione (DMDD) was isolated from the tuberous roots of Averrhoa carambola L. The purpose of this study was to investigate the beneficial effect of DMDD on the advanced glycation end-product-mediated renal injury in type 2 diabetic KKAy mice with regard to prove its efficacy by local traditional practitioners in the treatment of kidney frailties in diabetics. KKAy mice were orally administrated DMDD (12.5, 25, 50 mg/kg body weight/d) or aminoguanidine (200 mg/kg body weight/d) for 8 weeks. Hyperglycemia, renal AGE formation, and the expression of related proteins, such as the AGE receptor, nuclear factor-κB, transforming growth factor-ß1, and Nε-(carboxymethyl)lysine, were markedly decreased by DMDD. Diabetes-dependent alterations in proteinuria, serum creatinine, creatinine clearance, and serum urea-N and glomerular mesangial matrix expansion were attenuated after treatment with DMDD for 8 weeks. The activities of superoxide dismutase and glutathione peroxidase, which are reduced in the kidneys of KKAy mice, were enhanced by DMDD. These findings suggest that DMDD may inhibit the progression of diabetic nephropathy and may be a therapeutic agent for regulating several pharmacological targets to treat or prevent of diabetic nephropathy.

19.
J Environ Manage ; 277: 111388, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33002812

RESUMO

Land degradation due to soil salinity and sodicity is a serious concern in arid ecosystems. Despite the importance of conservation tillage in carbon sequestration and improving soil properties, its effect on saline-sodic soils under amendment application remains unknown. Therefore, the present study aimed to explore the combined effects of inorganic (sulfuric acid and gypsum) and organic (vermicompost) soil amendments and tillage systems (zero, reduced and deep tillage) on saline-sodic soil properties and wheat productivity. Deep tillage with vermicompost application significantly improved soil physical and chemical properties compared with control. Interestingly, integration between deep tillage and vermicompost decreased soil salinity and sodicity by 37% and 34%, respectively, compared with zero tillage and unamended soils. The application of vermicompost surpassed chemical amendments in the improvement of saline-sodic soils and consequently increased the growth and yield of wheat, provided that deep tillage was used as a suitable tillage system. Although deep tillage reduced soil organic carbon, application of vermicompost not only compensated this reduction, but also significantly increased soil organic carbon. This confirms the potential of combined deep tillage and vermicompost as a method for environmentally reclaiming saline-sodic soils.


Assuntos
Solo , Triticum , Agricultura , Carbono , Sequestro de Carbono , Ecossistema
20.
Front Genet ; 11: 558394, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33304379

RESUMO

The tea aphid, Aphis aurantii, has become one of the destructive pests in tea plantations in the tropics and subtropics. Very few functional studies have so far focused on the developmental and reproductive biology at a molecular level, because of the lack of comprehensive genetic information. Full-length transcriptomes represent a very highly efficient approach to obtain reference gene sequences in non-model insects. In the present study, the transcriptome of A. aurantii was comprehensively sequenced using PacBio Iso-Seq technology. A total of 46.8 Gb nucleotides and 15,938 non-redundant full-length transcripts were obtained, 13,498 (84.69%) of which were annotated into seven databases. Of these transcripts, 2,029 alternative splicing events and 15,223 simple sequence repeats were detected. Among these transcripts, 4,571 (28.68%) and 11,367 (71.32%) were long non-coding RNAs (lncRNAs) and protein-coding genes, respectively. Five hundred and ninety transcription factors were detected. The first full-length transcriptome represents a significant increase in the known genetic information of A. aurantii. It will assist the future functional study of genes involved in its development and reproduction.

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