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1.
Food Chem ; 306: 125627, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31610328

RESUMO

Effects of various concentrations of Kadozan (chitosan) treatment on storability and quality properties of harvested 'Fuyan' longans were investigated. Compared to the control samples, Kadozan treated-longans displayed lower fruit respiration rate, lower pericarp cell membrane permeability, pericarp browning index, pulp breakdown index, fruit disease index, and weight loss, but higher rate of commercially acceptable fruit, higher levels of pericarp chlorophyll, carotenoid, anthocyanin, flavonoid and total phenolics, higher amounts of pulp total soluble sugar, sucrose, total soluble solids, and vitamin C. These results revealed Kadozan treatment could increase storability and retain better quality of harvested longan fruit. Among different concentrations of Kadozan, the dilution of 1:500 (VKadozan: VKadozan + Water) showed the best results in storability and maintained the best quality of longans during storage. These findings demonstrated that Kadozan could be a facile and eco-friendly postharvest handling approach for increasing storability and lengthening shelf-life of harvested 'Fuyan' longan fruit.

2.
Food Chem ; 305: 125439, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31499287

RESUMO

Compared to the control longans, hydrogen peroxide (H2O2)-treated longans exhibited higher index of pulp breakdown, higher fruit respiration rate, higher activities of pulp phosphohexose isomerase (PGI), succinate dehydrogenase (SDH), cytochrome C oxidase (CCO), ascorbic acid oxidase (AAO) and polyphenol oxidase (PPO), but lower activity of pulp nicotinamide adenine dinucleotide kinase (NADK). H2O2-treated longans also exhibited lower total activities of pulp glucose-6-phosphate dehydrogenase (G-6-PDH) and 6-phosphogluconate dehydrogenase (6-PGDH), lower levels of pulp NADP(H), but higher levels of pulp NAD(H). These data indicated that H2O2-stimulated longan pulp breakdown was owing to a decreased proportion of pentose phosphate pathway (PPP), the increased proportions of Embden-Meyerhof-Parnas pathway (EMP), tricarboxylic acid (TCA) cycle and cytochrome pathway (CCP) in total respiratory pathways. These findings further revealed that H2O2 could enhance respiration rate, and thus accelerate pulp breakdown occurrence and shorten the shelf life of longan fruit.

3.
J Biosci Bioeng ; 2019 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-31711928

RESUMO

Trehalose synthase converts maltose into trehalose in a single conversion step via intramolecular transformation and is thus useful for industrial production. In this study, we synthesized a thermophilic trehalose synthase from Thermus antranikianii (TaTS), which was recombinantly expressed in Escherichia coli BL21(DE3). The recombinant TaTS showed the highest activity at pH 7.0 and 60°C, with the maximum trehalose yield (76.8%) obtained at pH 7.0 and 30°C. TaTS activity was stable over a wide pH and temperature range of 6-10 and 4-70°C, respectively, over 6 h of incubation. The enzyme activity was strongly inhibited by Co2+, Cu2+, Zn2+, sodium dodecyl sulfate, and Tris. TaTS showed a 1.48-fold higher catalytic efficiency (kcat/Km) for maltose than for trehalose. Overall, these results demonstrate the good application potential of the recombinant enzyme TaTS in the efficient conversion of trehalose from maltose, with superior environmental tolerance to other trehalose synthases reported.

4.
Biol Lett ; 15(10): 20190503, 2019 10 31.
Artigo em Inglês | MEDLINE | ID: mdl-31662063

RESUMO

A recurring theme in the evolution of tetrapods is the shift from sprawling posture with laterally orientated limbs to erect posture with the limbs extending below the body. However, in order to invade particular locomotor niches, some tetrapods secondarily evolved a sprawled posture. This includes moles, some of the most specialized digging tetrapods. Although their forelimb anatomy and posture facilitates burrowing, moles also walk long distances to forage for and transport food. Here, we use X-ray Reconstruction Of Moving Morphology (XROMM) to determine if the mole humerus rotates around its long axis during walking, as it does when moles burrow and echidnas walk, or alternatively protracts and retracts at the shoulder in the horizontal plane as seen in sprawling reptiles. Our results reject both hypotheses and demonstrate that forelimb kinematics during mole walking are unusual among those described for tetrapods. The humerus is retracted and protracted in the parasagittal plane above, rather than below the shoulder joint and the 'false thumb', a sesamoid bone (os falciforme), supports body weight during the stance phase, which is relatively short. Our findings broaden our understanding of the diversity of tetrapod limb posture and locomotor evolution, demonstrate the importance of X-ray-based techniques for revealing hidden kinematics and highlight the importance of examining locomotor function at the level of individual joint mobility.

5.
Food Chem ; 297: 124955, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31253342

RESUMO

This study aimed to investigate the effect of hydrogen peroxide (H2O2) on membrane lipids metabolism and its relation to pulp breakdown development of longan fruit during postharvest storage. Compared to the control longans, H2O2-treated longans showed higher pulp breakdown index, cell membrane permeability, and activities of phospholipase D (PLD), lipase and lipoxygenase (LOX). Moreover, H2O2-treated longans maintained higher levels of pulp phosphatidic acid (PA) and saturated fatty acids (SFA). However, H2O2-treated longans exhibited lower levels of pulp phosphatidylcholine (PC), phosphatidylinositol (PI) and unsaturated fatty acids (USFA), lower index of unsaturated fatty acids (IUFA), and lower ratio of USFA to SFA (U/S). These findings demonstrated that H2O2 caused the increased activities of enzymes involving in membrane lipids degradation and the accelerated decompositions of membrane USFA and phospholipids in longan pulp, which eventually triggered the destruction of the pulp cell membrane structure and the development of pulp breakdown in longans during storage.


Assuntos
Enzimas/metabolismo , Frutas/química , Peróxido de Hidrogênio/farmacologia , Lipídeos de Membrana/metabolismo , Sapindaceae/química , Enzimas/química , Ácidos Graxos/análise , Ácidos Graxos/química , Armazenamento de Alimentos , Frutas/efeitos dos fármacos , Frutas/metabolismo , Peróxido de Hidrogênio/química , Lipase/química , Lipase/metabolismo , Metabolismo dos Lipídeos/efeitos dos fármacos , Lipoxigenase/química , Lipoxigenase/metabolismo , Lipídeos de Membrana/química , Fosfolipase D/metabolismo , Fosfolipídeos/química , Fosfolipídeos/metabolismo , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Sapindaceae/efeitos dos fármacos , Sapindaceae/metabolismo
6.
Carbohydr Polym ; 217: 126-134, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31079668

RESUMO

Longan pulp is an excellent source of polysaccharides and other nutrients that have many health benefits. However, longans is susceptible to pulp breakdown after harvest and loses its nutrition values. To solve this problem, this study aimed to study the effects of a novel chitosan, Kadozan, on pulp breakdown index, contents of pectin, cellulose and hemicelluloses, and activities of enzymes in longan pulp relating to disassembly of polysaccharides (XET, PE, PG, ß-Gal, and cellulase). The data illustrated that, compared to the control longans, chitosan-treated longans contained higher amounts of CWM, CSP, ISP, cellulose and hemicelluloses, but exhibited lower pulp breakdown index, lower activities of cell wall-disassembling enzymes, and contained lower WSP amount. These results suggested that Kadozan with a dilution of 1:500 (VKadozan: VKadozan + Water) could significantly decrease activities of disassembling-enzymes and depolymerization of polysaccharides in cell wall, and subsequently alleviate pulp breakdown and prolong storage-life of postharvest longans.


Assuntos
Parede Celular/efeitos dos fármacos , Quitosana/farmacologia , Inibidores Enzimáticos/farmacologia , Frutas/metabolismo , Polissacarídeos/metabolismo , Sapindaceae/metabolismo , Parede Celular/metabolismo , Celulose/metabolismo , Conservação de Alimentos/métodos , Qualidade dos Alimentos , Glicosídeo Hidrolases/antagonistas & inibidores , Hidrólise/efeitos dos fármacos , Pectinas/metabolismo
7.
Food Chem ; 275: 239-245, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30724192

RESUMO

This study aimed to investigate the effects of Lasiodiplodia theobromae (Pat.) Griff. & Maubl (L. theobromae) inoculation on the energy status and activity of adenosine triphosphatase (ATPase) during L. theobromae-induced disease development and pericarp browning of harvested 'Fuyan' longan (Dimocarpus longan Lour. cv. Fuyan) fruit. The results showed that, compared to the control longans, L. theobromae-inoculated longans displayed higher indices of fruit disease and pericarp browning, lower pericarp ATP and ADP contents, higher AMP content, lower level of energy charge, as well as lower activities of Ca2+-ATPase, Mg2+-ATPase and H+-ATPase in membranes of plasma, vacuole, and mitochondria. These results indicated that the infection of L. theobromae reduced energy status and ATPase activities, caused ions disorder, damaged the integrity and function of the cell and organelles including vacuole and mitochondria in pericarp of longan fruit, which contributed to L. theobromae-promoted disease development and pericarp browning of harvested longan fruit during storage.


Assuntos
Adenosina Trifosfatases/metabolismo , Ascomicetos/patogenicidade , Doenças das Plantas/microbiologia , Sapindaceae/metabolismo , Sapindaceae/microbiologia , Difosfato de Adenosina/metabolismo , Trifosfato de Adenosina/metabolismo , Metabolismo Energético , Frutas/metabolismo , Frutas/microbiologia , Mitocôndrias/metabolismo , Proteínas de Plantas/metabolismo , Vacúolos/metabolismo
8.
J Exp Biol ; 222(Pt 4)2019 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-30718373

RESUMO

The interplay between morphological specialization and kinematic flexibility is important for organisms that move between habitats within different substrates. Burrowing is energetically expensive and requires substantial interaction with soil to dislodge and transport it. True moles (Talpidae) have extraordinary forelimb morphologies and a unique ability to dig in loose as well as compact soils, yet we know little of how moles coordinate their forelimb joint kinematics when digging in soils of different compactness. Using marker-based X-ray Reconstruction of Moving Morphology (XROMM), we tested the hypothesis that moles burrow using different forelimb kinematics in loose and compact substrates. We predicted that moles raise mounds of loose soil by performing powerful compacting strokes mainly with long-axis rotation of the humerus (i.e. pronation/supination), but shear compact soil away by performing scratching strokes involving amplified elbow extension, similar to most scratching diggers. We also predicted that in both types of substrate, moles displace soil rearward like other mammalian diggers. Our results support our hypothesis but not the predictions. Eastern moles (Scalopus aquaticus) move substrates upward using compacting strokes in loose substrates and outward from the body midline using scratching strokes in compact substrates; unlike the digging strokes of most mammalian forelimb diggers, the power-stroke of moles itself does not displace substrates directly rearward. Compacting and scratching strokes involve similar ranges of humeral pronation and retraction at the scapulohumeral (shoulder) joint, yet the movements at the elbow and carpal joints differ. Our results demonstrate that the combination of stereotypic movements of the shoulder joint, where the largest digging muscles are located, and flexibility in the elbow and carpal joints makes moles extremely effective diggers in both loose and compact substrates.

9.
Front Microbiol ; 9: 2466, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30386318

RESUMO

Phomopsis longanae Chi is a major pathogenic fungus that infects harvested longan fruit. This study aimed to investigate the effects of P. longanae on reactive oxygen species (ROS) metabolism and its relation to the pericarp browning and disease development of harvested longan fruit during storage at 28°C and 90% relative humidity. Results showed that compared to the control longans, P. longanae-inoculated longans displayed higher indexes of pericarp browning and fruit disease, higher O2 -. generation rate, higher accumulation of malondialdehyde (MDA), lower contents of glutathione (GSH) and ascorbic acid (AsA), lower 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability and reducing power in pericarp. In addition, P. longanae-infected longans exhibited higher activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) in the first 2 days of storage, and lower activities of SOD, CAT, and APX during storage day 2-5 than those in the control longans. These findings indicated that pericarp browning and disease development of P. longanae-infected longan fruit might be the result of the reducing ROS scavenging ability and the increasing O2 -. generation rate, which might lead to the peroxidation of membrane lipid, the loss of compartmentalization in longan pericarp cells, and subsequently cause polyphenol oxidase (PPO) and peroxidase (POD) to contact with phenolic substrates which result in enzymatic browning of longan pericarp, as well as cause the decrease of disease resistance to P. longanae and stimulate disease development of harvested longan fruit.

10.
J Agric Food Chem ; 2018 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-30403851

RESUMO

This study investigated the changes in metabolisms of membrane lipids and phenolics caused by Phomopsis longanae Chi infection in association with pericarp browning and fruit disease occurrence of postharvest longans. Compared with the uninoculated-longans, the longans inoculated by P. longanae exhibited higher cellular membrane permeability; higher PLD, lipase, and LOX activities; and higher levels of saturated fatty acids (SFAs) and phosphatidic acid but lower levels of phosphatidylinositol, phosphatidylcholine, and unsaturated fatty acids (USFAs). Additionally, the longans inoculated by P. longanae showed higher activities of POD and PPO but a lower amount of total phenolics. These findings suggested that infection of P. longanae enhanced activities of PLD-, lipase-, and LOX- stimulated degradations of membrane lipids and USFAs, which destroyed the integrity of the cell membrane structure, resulting in enzymatic browning by contact of phenolics with POD and PPO, and resulting in reduction of resistance to pathogen infection and accordingly accelerated disease occurrence of postharvest longan fruit.

11.
Food Chem ; 266: 299-308, 2018 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-30381189

RESUMO

This study aimed to investigate the effects of a novel chitosan formulation (Kadozan) treatment on disease development, response of disease resistance, metabolism of reactive oxygen species (ROS) in Peronophthora litchii-inoculated "Wuye" litchis. Compared with P. litchii-inoculated litchis, Kadozan-treated P. litchii-inoculated litchis exhibited lower fruit disease index, higher lignin content, higher activities of disease resistance-related enzymes (CHI, GLU and PAL), lower O2- generating rate and malondialdehyde content, higher activities of ROS scavenging enzymes (SOD, CAT and APX), higher contents of ascorbic acid and glutathione, and higher levels of reducing power and DPPH radical scavenging activity. These results suggest that Kadozan can be used to inhibit the growth of P. litchii in harvested litchis owning to the enhancement of disease resistance and ROS scavenging capacity, and decreases in O2- accumulation and membrane lipid peroxidation. Kadozan treatment can be used as a facile and novel method for suppressing postharvest pathogenic disease of litchis.

12.
Food Chem ; 269: 644-651, 2018 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-30100484

RESUMO

Compared to P. longanae-inoculated longan fruit, DNP-treated P. longanae-inoculated longans displayed higher fruit disease index, pericarp browning index and cell membrane permeability. Moreover, they exhibited higher activities of phospholipase D, lipase and lipoxygenase, lower amounts of phosphatidylcholine, phosphatidylinositol and USFA (unsaturated fatty acids) as well as higher amounts of phosphatidic acid and SFA (saturated fatty acids). Additionally, lower ratio of USFA to SFA and USFA index were shown in DNP-treated P. longanae-inoculated longans. However, ATP-treated P. longanae-inoculated longans exhibited the opposite results. These findings indicated that DNP stimulated longan pericarp browning and disease development caused by P. longanae resulted from the increases in activities of membrane lipids-degrading enzymes, promoting degradation of membrane phospholipids and USFA, and disruption of membrane structural integrity. Whereas, the opposite results observed in ATP-treated P. longanae-inoculated longans were due to the reduction in activities of membrane lipids-degrading enzymes and the maintenance of membrane structural integrity.

13.
Front Microbiol ; 9: 1454, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30018608

RESUMO

Longan fruit is a popular subtropical fruit with a relatively short shelf life at room temperature mainly due to pericarp browning and fungal infection. This study aimed to investigate the infection of Phomopsis longanae Chi in longan fruit and its effects on the storability and shelf life of longan fruit. The relationship between the energy metabolism of harvested longan fruit and disease development and pericarp browning was elucidated. Results show that P. longanae-inoculation accelerated the deterioration of longan fruit and caused pericarp browning. It also led to the energy deficit in pericarp of longan fruit, which was reflected as lower contents of ATP and ADP, higher AMP content, and lower energy charge as compared to the control samples. Additionally, P. longanae-infection reduced the activities of H+-ATPase, Ca2+-ATPase, and Mg2+-ATPase in plasma, vacuolar, and mitochondrial membranes during the storage period. The results demonstrate that P. longanae-infection led to disease development and pericarp browning in harvested longan fruit, which were due to the infection-induced energy deficit and low ATPase activity that caused disorders of ion transport and distribution, and damaged the structure and function of vacuole, mitochondria, and eventually the whole cells of fruit tissues.

14.
Food Chem ; 264: 1-8, 2018 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-29853352

RESUMO

Disassembly of cell wall polysaccharides accompanied with softening is very common in harvested fruits. To develop a facile postharvest approach, which can be used at ambient temperature, for suppressing softening and maintaining higher nutritive cell wall polysaccharides of Younai plums, influences of paper containing 1-methylcyclopropene (1-MCP) on firmness, activities of cell wall-degrading enzymes, and contents of cell wall polysaccharide in Younai plums during storage at 25 ±â€¯1 °C were investigated. As compared to the control plums, 1.2 µL·L-1 1-MCP-treated plums exhibited higher firmness, lower activities of cell wall-degrading enzymes (pectinesterase, polygalacturonase, cellulase and ß-galactosidase), higher contents of cell wall polysaccharides (sodium carbonate-soluble pectin, chelate-soluble pectin, cellulose, and hemicelluloses), and lower content of water-soluble pectin. The results suggested that paper containing 1-MCP, which was convenient to apply under ambient temperature, could significantly inhibit activities of cell wall degrading-enzymes and decrease disassembly of cell wall polysaccharides, and subsequently retard softening in Younai plums.


Assuntos
Ciclopropanos/farmacologia , Armazenamento de Alimentos/métodos , Polissacarídeos/metabolismo , Prunus domestica/efeitos dos fármacos , Prunus domestica/metabolismo , Hidrolases de Éster Carboxílico/metabolismo , Parede Celular/química , Parede Celular/efeitos dos fármacos , Parede Celular/metabolismo , Embalagem de Alimentos/métodos , Frutas/química , Frutas/efeitos dos fármacos , Frutas/metabolismo , Papel , Pectinas/química , Pectinas/metabolismo , Poligalacturonase/metabolismo , Polissacarídeos/química , Prunus domestica/química
15.
Front Microbiol ; 9: 1051, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29875756

RESUMO

The main goal of this study was to investigate the influences of Phomopsis longanae Chi infection on activities of cell wall-degrading enzymes (CWDEs), and contents of cell wall components in pericarp of harvested "Fuyan" longan (Dimocarpus longan Lour. cv. Fuyan) fruit and its relation to disease development. The results showed that, compared with the control samples, P. longanae-inoculated longans showed higher fruit disease index, lower content of pericarp cell wall materials (CWMs), as well as lower contents of pericarp cell wall components (chelate-soluble pectin (CSP), sodium carbonate-soluble pectin, hemicelluloses, and cellulose), but higher content of pericarp water-soluble pectin (WSP). In addition, the inoculation treatment with P. longanae significantly promoted the activities of CWDEs including pectinesterase, polygalacturonase, ß-galactosidase, and cellulase. The results suggested that the P. longanae stimulated-disease development of harvested longans was due to increase in activities of pericarp CWDEs, which might accelerate the disassembly of pericarp cell wall components. In turn, resulting in the degradation of pericarp cell wall, reduction of pericarp mechanical strength, and subsequently leading to the breakdown of longan pericarp tissues. Eventually resulting in development of disease development and fruit decay in harvested longans during storage at 28°C.

16.
J Biomed Nanotechnol ; 14(3): 430-455, 2018 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-29663919

RESUMO

Schistosomiasis is a neglected disease of tropics and subtropics, which has been associated with illness and death of millions of people, especially children. Although, excessive use of anthelmintic drugs and improvement of sanitation procedures have been in practice, yet timely detection, and diagnosis of low-intensity infections, are of utmost importance for reducing morbidity and final elimination of the disease. The diagnosis methods, currently being used for schistosomiasis, have several limitations, hence warrant further investigations for development of novel detection methods with higher rapidity, reliability, and convenience. Nanoparticles have widely been used in bioassays, because of their low-cost, high sensitivity and rapidity. Nano-diagnosis of schistosomiasis is a potential prospect, so this review described the application of nanotechnology for detection of antigen, antibody and the nucleic acid of Schistosoma. Furthermore, the techniques described in this review included magnetic affinity enzyme-linked immunoassay, colloidal gold, nanotechnology-based screen-printed biosensors, and electrochemical genosensors, etc. Moreover, the prospects and obstacles in the development of nanoparticle-based sensors for diagnosis of schistosomiasis were discussed. Finally, this study will provide the basis for nanoparticle-based detection and control of schistosomiasis, thus helping the improvement of public health.

17.
Food Chem ; 252: 134-141, 2018 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-29478523

RESUMO

The effects of Kadozan (a novel chitosan formulation) treatment on physiological attribute, nutritional quality and storage behavior of harvested "Wuye" litchi fruit were studied. Compared with control litchis, Kadozan treatment significantly decreased fruit respiration rate, retarded the increase of pericarp cell membrane permeability, maintained higher contents of anthocyanins and flavonoids and higher values of L∗, a∗ and b∗ in litchi pericarp, and reduced the decreases of titratable acidity, total soluble solids, total soluble sugars, and vitamin C contents in litchi pulp, maintaining better quality of litchis. Furthermore, Kadozan treatment decreased browning index and disease index of litchis, kept higher rate of commercially acceptable fruit, and reduced fruit weight loss, showing better storage behavior of litchis under ambient temperature. The optimal Kadozan treatment for litchis was the 1:100 (VKadozan: VKadozan + Water) dilution, which might be a promising method for keeping quality and prolonging shelf-life of harvested "Wuye" litchi fruit.


Assuntos
Quitosana/química , Quitosana/farmacologia , Qualidade dos Alimentos , Armazenamento de Alimentos/métodos , Frutas/química , Litchi/química , Composição de Medicamentos , Temperatura Ambiente
18.
Food Chem ; 244: 93-101, 2018 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-29120810

RESUMO

Effects of Lasiodiplodia theobromae inoculation on disease development, pericarp browning and membrane lipids metabolism of harvested "Fuyan" longan fruit were studied. Compared with control fruit, L. theobromae-inoculated longans showed higher fruit disease index, pericarp browning index and cell membrane permeability, as well as higher activities of phospholipase D, lipase and lipoxygenase. Additionally, there were lower contents of membrane phospholipids but higher content of phosphatidic acid, and lower level of unsaturated fatty acids but higher level of saturated ones with lower ratio of unsaturated fatty acid to saturated fatty acid and lower index of unsaturated fatty acids in pericarp of L. theobromae-inoculated longans. These results suggested that L. theobromae-induced disease development and pericarp browning of harvested longans might be attributed to the damaged cellular membrane structural integrity, induced by the activated membrane lipids-degrading enzymes increasing the degradation of membrane phospholipids and unsaturated fatty acids in pericarp of harvested longan fruit.


Assuntos
Frutas/metabolismo , Frutas/microbiologia , Lipídeos de Membrana/metabolismo , Doenças das Plantas/microbiologia , Sapindaceae/microbiologia , Spiroplasma/fisiologia , Lipoxigenase/metabolismo , Fosfolipase D/metabolismo , Fosfolipídeos/metabolismo
19.
Food Chem ; 240: 863-869, 2018 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-28946353

RESUMO

Effects of propyl gallate on metabolisms of respiration and energy of harvested 'Fuyan' longans and its relationship to pericarp browning were investigated. Compared to control longans, propyl gallate could reduce ascorbic acid oxidase (AAO) activity, lower cytochrome C oxidase (CCO) activity during early-storage and mid-storage, increase NADK activity, elevate contents of NADP and NADPH, decrease contents of NAD and NADH, in addition, lower the decreases of ATP content and energy charge (E.C.), increase activities of mitochondrial H+-ATPase, Ca2+-ATPase and Mg2+-ATPase during early-storage and mid-storage. Above results suggested that propyl gallate-retarded browning development in pericarp of harvested longans was resulted from decreases in activities of respiratory terminal oxidases like CCO and AAO, increase in proportion of pentose phosphate pathway (PPP) to Embden-Meyerhof pathway (EMP) and tricarboxylic acid (TCA) cycle, and maintenance of mitochondrial integrity via retaining higher levels of ATP content and energy charge, as well as higher activities of mitochondrial ATPase.


Assuntos
Sapindaceae , Frutas , Glicólise , Oxirredutases , Galato de Propila
20.
Food Chem ; 247: 16-22, 2018 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-29277223

RESUMO

Effects of Lasiodiplodia theobromae on reactive oxygen species (ROS) production-scavenging system during L. theobromae-induced pericarp browning and disease development of harvested "Fuyan" longans were investigated. Compared with control longans, L. theobromae-inoculated longans exhibited higher pericarp browning index and fruit disease index, higher pericarp O2- generation rate and MDA content. Moreover, L. theobromae infection also resulted in lower contents of pericarp AsA and GSH, lower levels of pericarp DPPH radical scavenging ability and reducing power. Additionally, L. theobromae infection decreased the activities of pericarp SOD, CAT and APX from day 2 to day 5. These findings suggested that L. theobromae-induced pericarp browning and disease development of harvested longans might be due to reduction of ROS scavenging ability and increase in ROS production, which might stimulate membrane lipid peroxidation, disrupt cellular membrane structure, and cause the loss of cellular compartmentalization and disease resistance, in turn, resulting in pericarp browning and disease development.


Assuntos
Ascomicetos/patogenicidade , Espécies Reativas de Oxigênio/metabolismo , Sapindaceae/metabolismo , Sapindaceae/microbiologia , Ascomicetos/metabolismo , Catalase/metabolismo , Resistência à Doença , Frutas/metabolismo , Frutas/microbiologia , Glutationa/metabolismo , Interações Hospedeiro-Patógeno , Doenças das Plantas/microbiologia , Proteínas de Plantas/metabolismo , Superóxido Dismutase/metabolismo
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