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1.
Sci Total Environ ; 833: 155164, 2022 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-35413340

RESUMO

Synthetic musks are aroma compounds used worldwide as substitutes for natural musks, which have become ubiquitous pollutants in the environment. The land-based input and partition behavior of synthetic musks in sediments and seawater are important for understanding their fate in the marine environment. In this study, a total of 292 seawater and sediment samples from 146 sites of the Yellow Sea and East China Sea were collected during wet and dry seasons and used to determine four synthetic musks, tonalide, galaxolide, musk xylene and musk ketone. Only tonalide was detectable in 10.6% and 17.8% of seawater samples at a concentration range of 23.7-38.2 ng/L and 19.0-24.8 ng/L in wet season and dry season, respectively. In sediments, both galaxolide and tonalide were detectable, with a sum total synthetic musks concentration ranging from 1.4 to 36.6 ng/g dry weight (dw) in wet season and 1.0 to 14.1 ng/g dw in dry season. The concentration of tonalide in seawater was significantly higher in wet season than in dry season and the concentration of synthetic musks in sediments of some coastal areas also exhibited higher concentrations in wet season than in dry season. Fugacity analysis showed that tonalide in the Yellow Sea exhibited a trend of diffusion from sediment to seawater or a state of equilibrium, while no diffusion trend was observed in the East China Sea. The hazard quotient values for tonalide and galaxolide in sediment and seawater samples were lower than 0.1, indicating that they present a low ecological risk. This research provides novel insights that help understand the pollution status, diffusion behavior and ecological risk of synthetic musks in marine environments.

2.
Sci Total Environ ; 832: 155118, 2022 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-35398136

RESUMO

Emerging contaminants (ECs) and heavy metals (HMs) are universally present together in estuarine sediments; despite this, their effects on microbial communities have been widely studied separately, rather than in consort. In this study, the combined effects of ECs and HMs on microbial communities were investigated in sediments from 11 major river estuaries around the Bohai Sea, China. Proteobacteria, Bacteroidetes, and Firmicutes were the dominant phyla in the sediments. Using Shannon indices, total phosphorus and total organic carbon were shown to affect microbial community structure. Redundancy analysis of microbial variation implicated Cd and As as the greatest pollutants, followed by Mn, Fe, Zn and Cu; no impacts from galaxolide (HHCB) and tonalide (AHTN) were found. Correlation analysis demonstrated that the concentration of ECs increased the abundance of certain bacteria (e.g., Haliangium, Altererythrobacter, Gaiella and Erythrobacter), and therefore these can be used as potential contamination indicators. Shannon indices and Chao1 indices showed that there were differences in the richness and diversity of bacterial communities in the sediments of 11 rivers. The principal coordinate analysis displayed higher similarity of bacterial community composition in estuarine sediments in Liaoning province than other regions. The results can be used to predict changes in estuary ecosystems to maintain their ecological balance and health.

3.
Front Pharmacol ; 13: 811808, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35479319

RESUMO

Background: Knee osteoarthritis (KOA) is a degenerative disease that develops over time. Icariin (ICA) has a positive effect on KOA, although the mechanism is unknown. To investigate drug-disease connections and processes, network pharmacology is commonly used. The molecular mechanisms of ICA for the treatment of KOA were investigated using network pharmacology, molecular docking and literature research approaches in this study. Methods: We gathered KOA-related genes using the DisGeNET database, the OMIM database, and GEO microarray data. TCMSP database, Pubchem database, TTD database, SwissTargetPrediction database, and Pharmmapper database were used to gather ICA-related data. Following that, a protein-protein interaction (PPI) network was created. Using the Metascape database, we performed GO and KEGG enrichment analyses. After that, we built a targets-pathways network. Furthermore, molecular docking confirms the prediction. Finally, we looked back over the last 5 years of literature on icariin for knee osteoarthritis to see if the findings of this study were accurate. Results: core targets relevant to KOA treatment include TNF, IGF1, MMP9, PTGS2, ESR1, MMP2 and so on. The main biological process involved regulation of inflammatory response, collagen catabolic process, extracellular matrix disassembly and so on. The most likely pathways involved were the IL-17 signaling pathway, TNF signaling pathway, Estrogen signaling pathway. Conclusion: ICA may alleviate KOA by inhibiting inflammation, cartilage breakdown and extracellular matrix degradation. Our study reveals the molecular mechanism of ICA for the treatment of KOA, demonstrating its potential value for further research and as a new drug.

4.
Stem Cells Dev ; 2022 Apr 19.
Artigo em Inglês | MEDLINE | ID: mdl-35316077

RESUMO

Clinical guidelines need high-quality studies to support clinical decision making, in which the evidence often was collected from systematic reviews (SRs) and/or meta-analyses (MAs). At present, the methodological quality and risk of bias (RoB) of SRs/MAs on stem cell therapy for the treatment of knee osteoarthritis (KOA) has been poorly investigated. This study aims to strictly evaluate the methodological quality and RoB in SRs/MAs of stem cell therapy for KOA. Four electronic databases (PubMed, Embase, Cochrane Library, and Web of Science databases) were searched, from inception to October 5, 2021. SRs/MAs involving randomized control trials or cohort studies on stem cell therapy for the treatment of KOA were included. The methodological quality and RoB were assessed using AMSTAR 2 and ROBIS tool, respectively. In total, 22 SRs/MAs were included. According to the results obtained by AMSTAR 2 tool, all SRs/MAs were rated as "Critically low." Main methodological weaknesses were as follows: up to 81.82% did not meet protocol registration requirements, only 13.64% provided a list of excluded studies and justification, and 13.64% investigated and discussed the publication bias. ROBIS-based RoB assessment showed that all the SRs/MAs were rated as "High." Besides, the lack of following the implementation of the PRISMA reporting guideline seems to reduce the methodological quality of the studies. The overall methodological quality of the SRs/MAs concerning the application of stem cell therapy in treating KOA is "Critically low," while the RoB is high. It is difficult to provide effective evidence for the formulation of guidelines for KOA treatment. We suggest that the relevant methodological quality assessment should be carried out in the future before the SRs/MAs are used as clinical evidence. In addition, it may be necessary for many journals to include the checklist with a submitted article. PROSPERO registration number: CRD42021246924.

5.
Plant Dis ; 2022 Feb 23.
Artigo em Inglês | MEDLINE | ID: mdl-35196099

RESUMO

Thinopyrum intermedium (JJJsJsStSt, 2n = 6x = 42), a wild relative of common wheat, possesses many desirable agronomic genes for wheat improvement. The production of wheat-Th. intermedium introgression lines is a key step for transferring these beneficial genes into wheat. In this study, we characterized three wheat-Th. intermedium introgression lines TA3681, TA5566, and TA5567 using non-denaturing fluorescence in situ hybridization (ND-FISH), genomic in situ hybridization (GISH), polymerase chain reaction (PCR)-based landmark unique gene (PLUG) and intron targeting (IT) markers. Our results showed that TA3681 is a wheat-Th. intermedium 1St disomic addition line, TA5566 is a wheat-Th. intermedium non-Robertsonian translocation line carrying two pairs of 3A-7Js translocation chromosomes, and TA5567 is a wheat-Th. intermedium non-Robertsonian translocation line carrying a pair of 3A-7Js translocation chromosomes. We developed 13, 36, and 15 Th. intermedium chromosome-specific markers for detecting the introgressed Thinopyrum chromosomes in TA3681, TA5566, and TA5567, respectively. Stem rust assessment revealed that TA3681 exhibited high level of seedling resistance to Chinese prevalent Puccinia graminis f. sp. tritici (Pgt) pathotypes, and both TA5566 and TA5567 were highly resistant to Australian Pgt pathotypes, indicating that Th. intermedium chromosomes 1St and 7Js might carry new stem rust resistance genes. Therefore, the new identified introgression lines may be useful for improving wheat stem rust resistance.

6.
Environ Res ; 207: 112673, 2022 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-34990603

RESUMO

With the frequent use of chemical pesticides, the current-use pesticides (CUPs) emerge and concentrate in the sea. The partition between the sediment and seawater is essential for understanding the environmental fate of CUPs. However, there is little research on this topic. In the present study, seventeen CUPs were screened in seawater and sediment samples collected from the Yellow Sea and East China Sea. Total concentration of 17 CUPs in surface seawater samples ranged from 9.5 to 267.3 ng/L, with 6 CUPs presenting 100% detection frequency. Carbendazim, tricyclazole, tebuconazole, atrazine and imidacloprid accounted for >80% of all CUPs, which was due to their large application in the local agriculture and fishing activities. Higher concentration sites were located near the shore and Yangtze river estuary, indicating intense human activities and riverine input that elevated the level of CUPs in marginal sea. The pesticides in seawater were mainly found in the surface followed by the bottom layer, which indicated that atmospheric deposition and re-suspension played key roles for their vertical distribution characteristics. The high fugacity fraction ratios (ff > 0.5) indicated the non-equilibrium state of pesticides that might have been transferred from sediment to seawater at most sites. These 17 detectable pesticides in seawater were at low levels, presenting ignorable or low toxic effects to aquatic organisms.


Assuntos
Praguicidas , Poluentes Químicos da Água , China , Monitoramento Ambiental , Humanos , Praguicidas/análise , Água do Mar/química , Poluentes Químicos da Água/análise
7.
Plant Dis ; 106(3): 864-871, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34645309

RESUMO

Wheat pathogens, especially those causing powdery mildew and stripe rust, seriously threaten yield worldwide. Utilizing newly identified disease resistance genes from wheat relatives is an effective strategy to minimize disease damage. In this study, chromosome-specific molecular markers for the 3Sb and 7Sb chromosomes of Aegilops bicornis were developed using PCR-based landmark unique gene primers for screening wheat-A. bicornis progenies. Fluorescence in situ hybridization (FISH) was performed to further identify wheat-A. bicornis progenies using oligonucleotides probes Oligo-pSc119.2-1, Oligo-pTa535-1, and Oligo-(GAA)8. After establishing A. bicornis 3Sb and 7Sb chromosome-specific FISH markers, Holdfast (common wheat)-A. bicornis 3Sb addition, 7Sb addition, 3Sb(3A) substitution, 3Sb(3B) substitution, 3Sb(3D) substitution, 7Sb(7A) substitution, and 7Sb(7B) substitution lines were identified by the molecular and cytological markers. Stripe rust and powdery mildew resistance, along with agronomic traits, were investigated to evaluate the breeding potential of these lines. Holdfast and Holdfast-A. bicornis progenies were all highly resistant to stripe rust, indicating that the stripe rust resistance might derive from Holdfast. However, Holdfast-A. bicornis 3Sb addition, 3Sb(3A) substitution, 3Sb(3B) substitution, and 3Sb(3D) substitution lines showed high resistance to powdery mildew while Holdfast was highly susceptible, indicating that chromosome 3Sb of A. bicornis carries previously unknown powdery mildew resistance gene(s). Additionally, the transfer of the 3Sb chromosome from A. bicornis to wheat significantly increased tiller number, but chromosome 7Sb has a negative effect on agronomic traits. Therefore, wheat germplasm containing A. bicornis chromosome 3Sb has potential to contribute to improving powdery mildew resistance and tiller number during wheat breeding.


Assuntos
Aegilops , Aegilops/genética , Cromossomos de Plantas/genética , Hibridização in Situ Fluorescente , Melhoramento Vegetal , Doenças das Plantas/genética , Triticum/genética
8.
Environ Pollut ; 302: 119017, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35192883

RESUMO

Organophosphates (OPEs) are manmade organic pollutants that are widely used as flame retardants, plasticizers, and antifoaming and hydraulic agents. In this study, seven OPEs in seawater and sediment from the Yellow Sea and East China Sea were determined to study the distribution and diffusion behavior, and to evaluate the environmental risks. The ΣOPEs in the seawater and sediments ranged from below the method detection limit (1.0, indicating high ecological risks to aquatic organisms.


Assuntos
Retardadores de Chama , China , Monitoramento Ambiental , Ésteres/análise , Retardadores de Chama/análise , Oceanos e Mares , Organofosfatos/análise , Água do Mar
9.
World Neurosurg ; 2021 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-34871803

RESUMO

OBJECTIVE: We critically evaluated the efficacy and safety of oblique lumbar interbody fusion (OLIF) versus transforaminal lumbar interbody fusion (TLIF) for degenerative lumbar spondylolisthesis (DLS). METHODS: PubMed, Embase, Cochrane Library, the Web of Science Core Collection, Chinese Biomedical Literature, China National Knowledge Infrastructure, Wanfang Digital Periodicals, and Chinese Science and Technology Periodicals were searched from their inception to February 2021. Randomized controlled trials and retrospective or prospective cohort studies (CSs) comparing OLIF and TLIF for DLS were included. A meta-analysis was conducted, if possible. RESULTS: Ten studies were included in the statistical analysis. The pooled results of the CSs showed no statistically significant differences (P > 0.05) in pain relief at 3 or 6 months of follow-up and functional improvement at 1 or 3 months of follow-up in DLS patients between those who had undergone OLIF versus TLIF. The pooled results of the CSs showed that OLIF could significantly improve the degree of lumbar lordosis, foraminal height, and disc height and decrease the intraoperative blood loss, postoperative drainage volume, operative duration, bed rest time, and hospital length of stay (P < 0.05) compared with TLIF. The incidence of adverse events was not significantly different statistically between OLIF and TLIF. CONCLUSIONS: The results from the present study suggest that pain relief and functional improvement were not significantly different between OLIF and TLIF. Nevertheless, the use of OLIF might improve radiological outcomes and reduce intraoperative blood loss, postoperative drainage volume, operative duration, bed rest duration, and hospital length of stay compared with TLIF. Additional high-quality randomized controlled trials are still required to confirm these findings.

10.
Int J Gen Med ; 14: 8481-8494, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34848997

RESUMO

BACKGROUND: Acupuncture has been widely used in the clinical management of osteoarthritis of the knee (KOA). Many systematic reviews (SRs) and meta-analyses (MAs) have reported its effectiveness in relieving pain. This overview aimed to summarize SRs and MAs on the effectiveness and safety of acupuncture for KOA and evaluate their methodological and evidence quality of the included SRs and MAs. METHODS: We conducted a comprehensive literature search for SRs and MAs in four Chinese and four international databases from their inception until August 2021. Two researchers independently searched the reviews, extracted the data, and cross-checked the data. The Assessing the Methodological Quality of Systematic Reviews 2 (AMSTAR 2) tool was used to evaluate the methodological quality of the included SRs and MAs. The Grades of Recommendations, Assessment, Development, and Evaluation (GRADE) system was used to assess the quality of evidence for the outcomes of the included SRs and MAs. RESULTS: A total of 14 SRs and MAs were included. The evaluation results of the AMSTAR 2 tool showed that the methodological quality of all the 14 SRs and MAs was critically low. The principal causes are the lack of a pre-registration proposal and a list of excluded studies and justify the exclusions, the report on the sources of funding, and the reasons for the study designs for inclusion. The results of the GRADE evaluation showed 25 of 46 outcomes were very low-level evidence. Seventeen were of low level, four were of moderate level and none were of high level. Most outcomes were downgraded in quality of evidence mainly because of publication bias and imprecision. CONCLUSION: The existing evidence suggests that acupuncture seems to be an effective and safe therapy for KOA. However, the deficiencies in the methodological quality and quality of evidence of the included SRs/MAs have limited the reliability of the conclusions. Therefore, further rigorous and comprehensive studies are warranted to verify the effectiveness and safety of acupuncture in KOA.

11.
Front Plant Sci ; 12: 708551, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34381484

RESUMO

Aegilops sharonensis, a wild relative of wheat, harbors diverse disease and insect resistance genes, making it a potentially excellent gene source for wheat improvement. In this study, we characterized and evaluated six wheat-A. sharonensis derivatives, which included three disomic additions, one disomic substitution + monotelosomic addition and two disomic substitution + disomic additions. A total of 51 PLUG markers were developed and used to allocate the A. sharonensis chromosomes in each of the six derivatives to Triticeae homoeologous groups. A set of cytogenetic markers specific for A. sharonensis chromosomes was established based on FISH using oligonucleotides as probes. Molecular cytogenetic marker analysis confirmed that these lines were a CS-A. sharonensis 2Ssh disomic addition, a 4Ssh disomic addition, a 4Ssh (4D) substitution + 5SshL monotelosomic addition, a 6Ssh disomic addition, a 4Ssh (4D) substitution + 6Ssh disomic addition and a 4Ssh (4D) substitution + 7Ssh disomic addition line, respectively. Disease resistance investigations showed that chromosome 7Ssh of A. sharonensis might harbor a new powdery mildew resistance gene, and therefore it has potential for use as resistance source for wheat breeding.

12.
Front Pharmacol ; 12: 611722, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34177561

RESUMO

Background: Icariin, a traditional Chinese medicine, plays a protective role in the treatment of exercise fatigue. Zinc, a trace element, plays an important role in the reproductive system. Therefore, we aimed to synthesize an Icariin-Zinc complex (by chemical means) and verify its protective effect on exercise fatigue and the reproductive system using animal experiments. Methods: The icariin-zinc complex was prepared by the reaction of icariin carbonyl and zinc ions (molar ratio 1:3). The molecular formula and structural formula of the complex were identified and tested. Fifty-six rats selected by swimming training were randomly divided into six groups: static control, exercise control, icariin, gluconate zinc (G-Zn group), icariin glucose zinc and icariin-zinc exercise ( low, high dose/L-E group, H-E group) groups. These groups respectively received the following doses: 1 ml/100 g, daily gavage with NS (for the first two groups), 45 mg/kg icariin, 110 mg/kg Gluconate Zinc, Icariin glucose zinc (45 mg/kg Icariin and 110 mg/kg Gluconate Zinc), 60 mg/kg icariin zinc and 180 mg/kg icariin zinc. After 3 weeks of gavage, we conducted 6 weeks of exhaustive swimming training. Test indices such as exhaustive swimming time of rats and body weight were evaluated after the last training exercise. The seminal vesicles, testes, and prostate gland were weighed, and their indices were calculated. The levels of testosterone (in the plasma) and glycogen (in the liver and muscle homogenates) were also evaluated using ELISA. Results: Compared with the static control group, the exhaustive swimming time of the rats in each group was prolonged. Compared with the other groups, the exhaustive swimming time of the L-E and H-E groups was significantly longer (p < 0.01); the Icariin-Zinc complex significantly increased the exhaustive swimming time of the rats. Compared with the static control group, the plasma testosterone content of the L-E and H-E groups increased significantly (p < 0.05). Compared with the exercise control group and G-Zn group, the plasma testosterone content of the H-E group also increased significantly (p < 0.01). The Icariin-Zinc complex significantly increased the serum levels of testosterone in rats. Compared with the control group, the muscle glycogen reserves of each group decreased, indicating that the muscle glycogen reserves of the rats decreased after swimming. Compared with other groups, the Icariin-Zinc complex can reduce the level of glycogen in the muscles, indicating that it can increase the utilization efficiency of glycogen in muscles. Compared with the static control and exercise control groups, the testicular weight of rats in the administration groups increased slightly. The Icariin-Zinc complex increased the testicular weight, indicating that the function of the reproductive system was improved to some extent. Conclusion: Icariin-Zinc can significantly prolong the exhaustive swimming time, improve exercise ability, and increase the plasma testosterone level (which is beneficial for improving the reproductive ability of male rats). Moreover, the beneficial effect of Icariin-Zinc on the glycogen content, testis index, and other reproductive system glands is dose-dependent.

13.
Am J Phys Med Rehabil ; 100(12): 1160-1169, 2021 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-33605578

RESUMO

OBJECTIVE: The aim of the study was to identify the effectiveness of the continuous passive motion application on clinical outcomes after total knee arthroplasty, based on evidence from recently published high-quality randomized controlled trials. DESIGN: Two reviewers retrieved platforms of PubMed, Embase, and CENTRAL independently, for identifying eligible randomized controlled trials evaluating the effect of continuous passive motion applied after total knee arthroplasty for knee osteoarthritis. Subgroup meta-analyses were performed for all syntheses based on the follow-up intervals. RESULTS: A total of 10 randomized controlled trials, involving 841 patients, were finally included. Data were available for 15 different outcomes (including active/passive knee extension/flexion/full range of motion, Western Ontario and McMaster Universities Osteoarthritis Index-pain/physical function/stiffness/total score, visual analogue scale, time up and go, knee girth, Knee Society Scale-function/knee score), at several time points. In general, most of the pools demonstrated similar outcome between continuous passive motion and noncontinuous passive motion groups. Exclusively, the active knee extension at 1 wk (mean difference = 3.00, 95% confidence interval = 0.5-5.5, P = 0.019*), passive knee extension at 1 wk (mean difference = 3.00, 95% confidence interval = 0.28-5.72, P = 0.031*), and 3 mos (mean difference = 3.00, 95% confidence interval = 0.5-5.5, P = 0.019*) were shown to be significantly slightly different between two groups. CONCLUSIONS: This study demonstrated a limited role of continuous passive motion in patients operated with total knee arthroplasty. Thus, there is at this stage no indication for continuous passive motion procedures in patients operated with total knee arthroplasty as a standard postoperative care.


Assuntos
Artroplastia do Joelho/reabilitação , Terapia Passiva Contínua de Movimento/métodos , Osteoartrite do Joelho/cirurgia , Humanos , Ensaios Clínicos Controlados Aleatórios como Assunto , Amplitude de Movimento Articular
14.
Food Chem ; 350: 129214, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-33601093

RESUMO

Tetrabromobisphenol A (TBBPA), halogenated carbazole (HCZ), and their analogs are the emerging pollutants invading the marine environment. So far, a few methods have been reported for the simultaneous analysis of these pollutants due to their large polarity difference. In this study, an effective extraction and cleanup strategy was developed for the simultaneous determination of 19 TBBPA and HCZ congeners in seafood. The 19 analytes could be directly analyzed through high performance liquid chromatography after ultrasonic extraction (methanol, duplicate ethyl acetate-acetone (1:1, v/v)) and gel permeation chromatography cleanup. The acceptable spike-recoveries were within 65.7-118.3%; the precision was intra-/inter-day RSDs: 0.0-6.7%/0.0-8.5%; and the matrix effects were between -14.1% and 12.4%. The detection limits and quantification limits were 0.002-0.014 and 0.020-0.200 µg g-1 dw, respectively. Additionally, this method successfully analyzed the seafood samples and the concentrations of these analytes were in range of nd-5.4 µg g-1 dw.


Assuntos
Carbazóis/análise , Fracionamento Químico/métodos , Análise de Alimentos/métodos , Halogenação , Bifenil Polibromatos/análise , Bifenil Polibromatos/isolamento & purificação , Alimentos Marinhos/análise , Carbazóis/química , Poluentes Ambientais/análise , Poluentes Ambientais/química , Contaminação de Alimentos , Fatores de Tempo
15.
J Orthop Surg Res ; 16(1): 23, 2021 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-33413543

RESUMO

BACKGROUND: Postmenopausal osteoporosis (PMO) that results from estrogen withdrawal is the most common primary osteoporosis among older women. However, little is known about the mechanism of PMO, and effective treatment of PMO is limited. METHODS: We used real-time polymerase chain reaction (qPCR), Western blotting, and RNA pull down to investigate the relationship between miR-186 and MOB Kinase Activator 1A (Mob1). Also, we investigated the effect of exosome in osteogenesis using alkaline phosphatase (ALP) staining. And hematoxylin eosin (HE) staining was used to verify the osteogenesis in PMO model. RESULTS: Exosomal miR-186 plays an important role in bone formation. The results of miRNA-seq and q-PCR showed that miR-186 was upregulated in a PMO + Exo treatment group. Results of RNA-pull down and luciferase reporter assays verified interactions between miR-186 and Mob1. We also verified the Hippo signaling pathway plays an important role in osteogenesis. CONCLUSIONS: We concluded that exosomes derived from human bone marrow mesenchymal stem cells (hBMSCs) can transfer miR-186 to promote osteogenesis in ovariectomy (OVX) rats through the Hippo signaling pathway.


Assuntos
Exossomos/genética , Exossomos/metabolismo , Células-Tronco Mesenquimais/citologia , MicroRNAs/fisiologia , Osteogênese/genética , Osteogênese/fisiologia , Osteoporose Pós-Menopausa/genética , Osteoporose Pós-Menopausa/fisiopatologia , Transdução de Sinais/genética , Transdução de Sinais/fisiologia , Animais , Modelos Animais de Doenças , Feminino , Humanos , MicroRNAs/metabolismo , Ovariectomia , Ratos Sprague-Dawley
16.
HIV Med ; 22(6): 467-477, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-33511687

RESUMO

OBJECTIVES: An intervention developed through participatory crowdsourcing methods increased HIV self-testing among men who have sex with men [MSM; relative risk (RR) = 1.89]. We estimated the long-term impact of this intervention on HIV transmission among MSM in four cities (Guangzhou, Shenzhen, Jinan and Qingdao). METHODS: A mathematical model of HIV transmission, testing and treatment among MSM in China was parameterized using city-level demographic and sexual behaviour data and calibrated to HIV prevalence, diagnosis and antiretroviral therapy (ART) coverage data. The model was used to project the HIV infections averted over 20 years (2016-2036) from the intervention to increase self-testing, compared with current testing rates. RESULTS: Running the intervention once would avert < 2.2% infections over 20 years. Repeating the intervention (RR = 1.89) annually would avert 6.4-10.7% of new infections, while further increases in the self-testing rate (hypothetical RR = 3) would avert 11.7-20.7% of new infections. CONCLUSIONS: Repeated annual interventions would give a three- to seven-fold increase in long-term impact compared with a one-off intervention. Other interventions will be needed to more effectively reduce the HIV burden in this population.


Assuntos
Infecções por HIV , Minorias Sexuais e de Gênero , China/epidemiologia , Infecções por HIV/diagnóstico , Infecções por HIV/epidemiologia , Infecções por HIV/prevenção & controle , Teste de HIV , Homossexualidade Masculina , Humanos , Masculino , Comportamento Sexual
17.
Int Orthop ; 45(2): 381-390, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-32556386

RESUMO

BACKGROUND: The aim of this study was to use umbilical cord mesenchymal stem cells (UCMSCs) loaded with graphene oxide (GO) granular lubricant to treat knee osteoarthritis (KOA) animal models and to analyze their effect on cytokine levels in the articular cavity. METHODS: Twenty-four New Zealand rabbit models of KOA were established by the modified Hulth and cartilage injury method, and they were assigned to the blank group, the GO group, the UCMSC group, and the GO + UCMSC group, each group containing six animal models. The GO and UCMSC groups were treated by a single intra-articular injection. The treatment was started one month after surgical modeling, and the observation period was eight weeks. The expression levels of nitric oxide (NO), interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α), glycosaminoglycan (GAG), and collagen-II (COL-II) in serum and articular fluid after treatment were compared to analyze the efficacy. RESULTS: The GO granular lubricant caused no significant improvement in the intra-articular environment of the knee joint, and UCMSCs caused a certain degree of improvement in the inflammatory environment. The improvement results of NO, IL-6, TNF-α, GAG, and COL-II were the best in the GO + UCMSC group, but the improvement results of inflammatory cytokine levels in serum and articular fluid were not consistent, especially the differences in NO, IL-6, and TNF-α were greater. CONCLUSION: UCMSCs loaded with the GO granular lubricant can reduce the inflammatory level and improve the level of biochemical environment in the articular cavity, and thus promote cartilage repair.


Assuntos
Cartilagem Articular , Células-Tronco Mesenquimais , Osteoartrite do Joelho , Animais , Citocinas , Grafite , Lubrificação , Modelos Animais , Osteoartrite do Joelho/terapia , Coelhos , Cordão Umbilical
18.
J Colloid Interface Sci ; 581(Pt B): 485-495, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32810725

RESUMO

Layered double hydroxides (LDHs) have been considered as one class of promising active electrode materials for supercapacitors due to their tunable composition and chemical versatility. Nonetheless, the poor electrical conductivity hinders their further practical applications in supercapacitors. Herein, CoAl LDH flower-like hollow microspheres are decorated with Ag nanoparticles by a facile one-step solvothermal reaction, followed by chemical bath deposition reaction. Experimental results and theoretical calculations indicate that decorating Ag nanoparticles onto CoAl LDH not only reduces the energy band gap and enhances their electrical conductivity, but also promotes fast diffusion kinetics of electrolyte ions and electrochemical reaction activity. Consequently, the prepared Ag/CoAl LDH electrode demonstrates improved specific capacities of 1214 (825) C g-1 at 3 (30) A g-1 and 91% capacity retention over 10,000 cycles at 10 A g-1 compared to the pristine CoAl LDH electrode. Moreover, using Ag/CoAl LDH and N-doped carbon nanotubes as the positive and negative electrodes, respectively, the assembled hybrid capacitor device delivers an energy density of 61.2 Wh kg-1 at a power density of 800 W kg-1. This work may showcase a great promise of engineering conductive nanoparticles-decorated LDHs-based active materials towards high-performance supercapacitors.

19.
Front Endocrinol (Lausanne) ; 12: 822294, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-35095776

RESUMO

Aim: This study is to investigate the effects of umbilical cord mesenchymal stem cells (UCMSCs) loaded with the graphene oxide (GO) granular lubrication on ameliorating inflammatory responses and osteoporosis of the subchondral bone in knee osteoarthritis (KOA) animal models. Methods: The KOA animal models were established using modified papain joint injection. 24 male New Zealand rabbits were classified into the blank control group, GO group, UCMSCs group, and GO + UCMSCs group, respectively. The concentration in serum and articular fluid nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), type II collagen (COL-II), and glycosaminoglycan (GAG) was detected using ELISA, followed by the dissection of femoral condyles and staining of HE and Micro-CT for observation via the microscope. Results: GO granular lubrication and UCMSCs repaired the KOA animal models. NO, IL-6, TNF-α, GAG, and COL-II showed optimal improvement performance in the GO + UCMSCs group, with statistical significance in contrast to the blank group (P <0.01). Whereas, there was a great difference in levels of inflammatory factors in serum and joint fluid. Micro-CT scan results revealed the greatest efficacy of the GO + UCMSCs group in improving joint surface damage and subchondral bone osteoporosis. HE staining pathology for femoral condyles revealed that the cartilage repair effect in GO + UCMSCs, UCMSCs, GO, and blank groups were graded down. Conclusion: UCMSCs loaded with graphene oxide granular lubrication can promote the secretion of chondrocytes, reduce the level of joint inflammation, ameliorate osteoporosis of the subchondral bone, and facilitate cartilage repair.


Assuntos
Cartilagem Articular , Células-Tronco Mesenquimais , Osteoartrite do Joelho , Osteoporose , Animais , Osso e Ossos , Grafite , Injeções Intra-Articulares , Lubrificação , Masculino , Osteoartrite do Joelho/patologia , Osteoporose/patologia , Papaína , Coelhos , Cordão Umbilical
20.
Artigo em Inglês | MEDLINE | ID: mdl-33123213

RESUMO

BACKGROUND: The efficacy of acupuncture for acute ankle sprain (AAS) is controversial. This study aimed to critically assess the efficacy and safety of acupuncture for AAS. METHODS: Parallel-group randomized controlled trials (RCTs) were included regardless of language or publication date. Participants with AAS were included regardless of age, sex, race, nationality, or diagnostic criteria for AAS. Experimental interventions included acupuncture alone or in combination with traditional therapies. Control interventions included no treatment, placebo, or traditional therapies. The primary outcome was the Kofoed ankle score. The secondary outcomes included visual analogue scale, duration of pain, use of painkiller, ankle circumference, effective rate, cure rate, and adverse events. PubMed, Embase, Cochrane Library, Web of Science, China National Knowledge Infrastructure, Wanfang Digital Periodicals, and Chinese Science and Technology Periodicals database were searched to identify potentially eligible studies from inception to September 10, 2020. World Health Organization International Clinical Trials Registry Platform (WHO ICTRP), ClinicalTrials.gov, Chinese Clinical Trial Registry (ChiCTR), and the reference list of eligible RCTs were checked to identify ongoing or unpublished studies. Risk of bias was assessed by the Cochrane Collaboration's tool. Statistical analyses were performed by RevMan 5.3 software. P < 0.05 indicated statistical significance. RESULTS: Seventeen eligible studies were included for the statistical analysis. There was no statistically significant difference of Kofoed ankle score between acupuncture and Rest, Ice, Compression, and Elevation (RICE) group (P=0.75). However, acupuncture could significantly relieve pain (P=0.02) and increase cure rate (P=0.004) compared with RICE. Moreover, acupuncture plus RICE could also significantly relieve pain (P < 0.00001) and increase cure rate (P=0.01) compared with RICE alone. Acupuncture combined with massage could significantly relieve pain (P=0.04) compared with massage alone. Acupuncture plus Chinese medicine might be more effective for relieving pain (P < 0.00001), reducing the duration of pain (P < 0.00001), and increasing cure rate (P=0.0002) compared with Chinese medicine alone. Two studies reported no adverse reactions. One study reported that a participant suffered from mild drug-related allergic reaction and was healed without treatment. CONCLUSIONS: The findings of the present study suggest that acupuncture may be beneficial for AAS. However, more large-scale and well-designed RCTs are warranted.

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