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1.
Bioengineered ; 11(1): 524-535, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32352854

RESUMO

Long non-coding RNAs (lncRNAs) play a key role in the development and metastasis of cancer. However, the biological role and clinical significance of lncRNA DNAJC3-AS1 in the development of colon cancer is still unknown. In this study, the effects of DNAJC3-AS1 on cell proliferation, migration, and invasion were evaluated by MTT assay, wound-healing assay, and transwell assay, respectively. The relationship between DNAJC3-AS1, miR-214-3p and LIVIN was predicted by the online software and confirmed by dual-luciferase reporter assay. We found that the down-regulation of DNAJC3-AS1 inhibited the proliferation of colon cancer cells and induced growth arrest. Down-regulation of DNAJC3-AS1 also inhibited the migration, invasion, and epithelial-mesenchymal transition (EMT) of colon cancer cells. Moreover, miR-214-3p can bind to DNAJC3-AS1, and knockdown of DNAJC3-AS1 increased miR-214-3p expression in colon cancer cells. LIVIN was identified as a target of miR-214-3p. The up-regulation of miR-214-3p inhibited the protein expression of LIVIN and suppressed the activation of the NF-κB signaling pathway. Besides, down-regulation of DNAJC3-AS1 reduced cell viability, invasion, and EMT of colon cancer cells, while miR-214-3p inhibitor could reverse these effects. The expression of LIVIN and the activation of the NF-κB signaling pathway were suppressed by down-regulating DNAJC3-AS1, while these effects could be restored by miR-214-3p inhibitor. These findings suggested that DNAJC3-AS1 may promote colon cancer progression by regulating the miR-214-3p/LIVIN axis. DNAJC3-AS1 may serve as a new biomarker and therapeutic target for colon cancer, stimulating new research directions and treatment options.

2.
Pharmacol Res ; 157: 104845, 2020 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-32353588

RESUMO

Cardiac injury is followed by fibrosis, characterized by myofibroblast activation. Excessive deposition of extracellular matrix (ECM) impairs the plasticity of myocardium and results in myocardial systolic and diastolic dysfunction. Mangiferin is a xanthonoid derivative rich in plants mangoes and iris unguicularis, exhibiting the ability to ameliorate metabolic disorders. This study aims to investigate whether mangiferin attenuates cardiac fibrosis via redox regulation. The transverse aortic constriction (TAC) in mice induced cardiac fibrosis with impaired heart function. Oral administration of mangiferin (50 mg/kg, 4 weeks) inhibited myofibroblast activation with reduced formation of ECM. The impaired left ventricular contractive function was also improved by mangiferin. TGF-ß1 stimulation increased glutaminolysis to fuel intracellular glutamate pool for the increased demands of nutrients to support cardiac myofibroblast activation. Mangiferin degraded Keap1 to promote Nrf2 protein accumulation by improving its stability, leading to Nrf2 activation. Nrf2 transcriptionally promotes the synthesis of antioxidant proteins. By activating Nrf2, mangiferin promoted the synthesis of glutathione (GSH) in cardiac fibroblasts, likely due to the consumption of glutaminolysis-derived glutamate as a source. Meanwhile, mangiferin promoted the exchange of intracellular glutamate for the import of extracellular cystine to support GSH generation. As a result of redistribution, the reduced glutamate availability failed to support myofibroblast activation. In support of this, the addition of extracellular glutamate or α-ketoglutarate diminished the inhibitory effects of mangiferin on cardiac myofibroblast proliferation and activation. Moreover, cardiac knockdown of Nrf2 attenuated the cardioprotective effects of mangiferin in mice subjected to TAC. In conclusion, we demonstrated that activated myofibroblasts were sensitive to glutamate availability. Mangiferin activated Nrf2 and redistributed intracellular glutamate for the synthesis of GSH, consequently impairing cardiac myofibroblast activation due to decreased glutamate availability. These results address that pharmacological activation of Nrf2 could restrain cardiac fibrosis via metabolic regulation.

3.
Nat Commun ; 11(1): 1818, 2020 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-32286268

RESUMO

Fast, robust and technology-independent computational methods are needed for supervised cell type annotation of single-cell RNA sequencing data. We present SciBet, a supervised cell type identifier that accurately predicts cell identity for newly sequenced cells with order-of-magnitude speed advantage. We enable web client deployment of SciBet for rapid local computation without uploading local data to the server. Facing the exponential growth in the size of single cell RNA datasets, this user-friendly and cross-platform tool can be widely useful for single cell type identification.

4.
J Neurol Sci ; 413: 116805, 2020 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-32259708

RESUMO

Separated ventral and dorsal streams in auditory system have been proposed to process sound identification and localization respectively. Despite the popularity of the dual-pathway model, it remains controversial how much independence two neural pathways enjoy and whether visual experiences can influence the distinct cortical organizational scheme. In this study, representational similarity analysis (RSA) was used to explore the functional roles of distinct cortical regions that lay within either the ventral or dorsal auditory streams of sighted and early blind (EB) participants. We found functionally segregated auditory networks in both sighted and EB groups where anterior superior temporal gyrus (aSTG) and inferior frontal junction (IFJ) were more related to the sound identification, while posterior superior temporal gyrus (pSTG) and inferior parietal lobe (IPL) preferred the sound localization. The findings indicated visual experiences may not have an influence on this functional dissociation and the cortex of the human brain may be organized as task-specific and modality-independent strategies. Meanwhile, partial overlap of spatial and non-spatial auditory information processing was observed, illustrating the existence of interaction between the two auditory streams. Furthermore, we investigated the effect of visual experiences on the neural bases of auditory perception and observed the cortical reorganization in EB participants in whom middle occipital gyrus was recruited to process auditory information. Our findings examined the distinct cortical networks that abstractly encoded sound identification and localization, and confirmed the existence of interaction from the multivariate perspective. Furthermore, the results suggested visual experience might not impact the functional specialization of auditory regions.

5.
Med Phys ; 2020 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-32302413

RESUMO

PURPOSE: This work aims to establish a classification framework for the diagnosis of mild cognitive impairment (MCI) at different stages (early MCI and late MCI) through direct analysis of resting-state functional magnetic resonance imaging (rs-fMRI) signals and using the accuracy (total correct rate), specificity (correct rate of late MCI) and sensitivity (correct rate of early MCI) to validate its classification performance. METHODS: All fMR images of subjects were parcellated into 116 regions of interest (ROIs) by applying the Anatomical Automatic Labeling (AAL) template, and the average rs-fMRI signals of each ROI were extracted. The Hilbert-Huang transform (HHT) was introduced into the framework to decompose each rs-fMRI signal into a series of intrinsic mode functions (IMFs) and to analyze these nonstationary and nonlinear time-series from the perspective of multiresolution. After obtaining the instantaneous frequencies and amplitudes of all IMFs of a signal, the Hilbert weighted frequencies (HWFs) were calculated and combined into a vector as the feature of the corresponding ROI. Support Vector Machine (SVM) was implemented to classify MCI at different stages. We used the independent two-sample t-test as the feature selection method and measured the classification performance through the leave-one-out cross-validation (LOOCV) method. RESULTS: Results on 77 early MCI (eMCI) and 64 late MCI (lMCI) with baseline rs-fMRI data from the Alzheimer's Disease Neuroimaging Initiative (ADNI) yielded 87.94% classification accuracy. Some of the brain regions with significant differences found by previous studies have been confirmed in this work. We found that HWF characteristics exhibited a significant downward trend in all cerebellar regions. The rs-fMRI signals in differential brain regions have not changed completely, but only altered in some narrow frequency bands. The analysis results showed that during the progress of MCI, the main changes of rs-fMRI were concentrated in IMF3, while IMFs with other indexes also contained HWF features with high SVM weights, such as Orbitofrontal superior frontal gyrus in IMF2, Insula in IMF4, and Lobule Ⅲ of vermis in IMF5, indicating that other IMFs provide important information for the diagnosis of MCI as well. CONCLUSIONS: This work confirmed the classification ability of HHT-based classification framework in classification of at different stages of MCI. Through the analysis, we found that during the progress of MCI the main changes of rs-fMRI were concentrated in IMF3, and HWF characteristics showed a significant downward trend in all cerebellar regions.

6.
Sci Rep ; 10(1): 5057, 2020 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-32193455

RESUMO

Sarcopenia is characterized by low skeletal muscle, a complex trait with high heritability. With the dramatically increasing prevalence of obesity, obesity and sarcopenia occur simultaneously, a condition known as sarcopenic obesity. Fat mass and obesity-associated (FTO) gene is a candidate gene of obesity. To identify associations between lean mass and FTO gene, we performed a genome-wide association study (GWAS) of lean mass index (LMI) in 2207 unrelated Caucasian subjects and replicated major findings in two replication samples including 6,004 unrelated Caucasian and 38,292 unrelated Caucasian. We found 29 single nucleotide polymorphisms (SNPs) in FTO significantly associated with sarcopenia (combined p-values ranging from 5.92 × 10-12 to 1.69 × 10-9). Potential biological functions of SNPs were analyzed by HaploReg v4.1, RegulomeDB, GTEx, IMPC and STRING. Our results provide suggestive evidence that FTO gene is associated with lean mass.

7.
Anal Chim Acta ; 1106: 161-167, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-32145844

RESUMO

A novel sensitive assay was established by using strand displacement amplification (SDA) and DNA G-quadruplex with aggregation-induced emission (AIE) for the detection of patulin (PAT) toxin. The complementary DNA (cDNA) of the aptamer and PAT competed for binding to aptamer-modified magnetic beads. The cDNA was obtained by magnetic separation and used as a primer in SDA to produce a large amount of G-base single-stranded DNA (ssDNA). They can form the G-quadruplex to be combined with the AIE of TTAPE dye, which features a special combination of G-quadruplex that amplify the fluorescent signals. This work can reach a lower detection limit of 0.042 pg mL-1 with a wide linear range of 0.001-100 ng mL-1 for PAT detection than other methods. The results also showed good recoveries of 97.8%-104% and 101.7%-105.3% in spiked apple and grape juices, respectively. The assay used for the detection of PAT exhibits high sensitivity and good specificity. It also provides a stable and reliable platform for detecting other small-molecule toxins.

8.
J Neural Eng ; 17(2): 026039, 2020 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-32163933

RESUMO

OBJECTIVE: Previous studies have reported that working memory (WM) may be affected by emotions and that the effect may exist in different stages of WM. However, at present it remains controversial whether emotions inhibit or facilitate WM, and how the mechanism of dynamic information transmission in the brain during WM is affected by emotions. APPROACH: In this study, we used a video database to induce three emotions (negative, neutral, and positive) and adopted a change detection paradigm based on electroencephalography. Event-related potential (ERP) analysis, event-related spectral perturbation analysis, source location analysis based on the dipole localization method and the distributed source localization method, and effective connectivity analysis were performed. MAIN RESULTS: Both behavioral and ERP results suggest that positive emotions have no significant effect on WM capacity, while negative emotions could facilitate WM capacity. Furthermore, the effective connectivity results based on two source location methods suggest that the long-range connectivity between the frontal and posterior areas can reflect the influence of positive and negative emotions on the WM network, in which the connectivity under the positive emotion condition occurs in the earlier period of WM maintenance, while the connectivity under the negative emotion condition occurs in the later period of WM maintenance. SIGNIFICANCE: The consistency of the behavioral, ERP, and effective connectivity results suggests that under the negative emotion condition, the top-down attention modulation between the frontoparietal area and posterior area could promote the most relevant information storage during WM maintenance.

9.
Brain Imaging Behav ; 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-32141031

RESUMO

Action recognition is an essential component of our daily life. The occipitotemporal cortex (OTC) is an important area in human movement perception. The previous studies have revealed that three vital regions including the extrastriate body area (EBA), human middle temporal complex (hMT+), and posterior superior temporal sulcus (pSTS) in OTC play an important role in motion perception. The aim of the current study is to explore the neural interactions between these three regions during basic human movement perception. Functional magnetic resonance imaging data were acquired when participants viewed dynamic videos depicting basic human movements. By the dynamic causal modeling analysis, a model space consisting of 576 models was constructed and evaluated to select the optimal model given the data. The information of the visual movement was found to enter the system through hMT+. We speculated that hMT+ would be the region to show sensitivity to the presence of motion and it subsequently influence and be influenced by the other two regions. Our results also revealed the manner in which the three regions interact during action recognition. Furthermore, We found significantly enhanced modulated connectivity from hMT+ to both EBA and pSTS, as well as from EBA to both hMT+ and pSTS. We inferred that there may be multiple routes for human action perception. One responsible route for processing motion signals is through hMT+ to pSTS, and the other projects information to pSTS may be via the form-processing route. In addition, pSTS may integrate and mediate visual signals and possibly convey them to distributed areas to maintain high-order cognitive tasks.

10.
Environ Sci Pollut Res Int ; 27(10): 10802-10810, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31950412

RESUMO

Sediments core within the Yangtze River Estuary was collected for metal and grain size analysis. The vertical distribution characteristics of eight metals along the core were investigated based on 137Cs and 210Pb radionuclide dating. The sediment was mainly composed of sand and silt. The metals concentrations were Al, 4.67-6.83; Fe, 2.3-3.94; Mn, 0.046-0.07; Cr, 69.5-103; Cu, 14.3-32.1; Zn, 47.3-96.7; Cd, 0.037-0.212; Pb, 13.7-23; Ni, 18.8-38.9 (mg·kg-1, except Al, Fe, and Mn as %), respectively. Geoaccumulation indexes (Igeo) indicated that Cu, Zn, and Pb were of pollution-free level; Cd, Cr, and Ni were in a slight polluted level. Based on potential ecological risk factors (EI), Cd posed a moderate risk to the local environment. Correlation analysis showed that Fe, Al, and Mn had a close association with Cu, Zn, Pb, and Ni at p < 0.01. Clay was significantly correlated with other metals except Cr and Cd.

11.
Neurosci Lett ; 718: 134746, 2020 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-31923522

RESUMO

Many studies have focused on the processing mechanism of sound directions in the human brain, however, as far as we know, it remains unclear whether the representations of sounds from different directions are different. In the present study, 28 subjects were scanned while listening to sounds from different directions. We used the whole-brain functional connectivity (FC) analysis to explore which brain regions had significant changes. Our results revealed that sounds from different directions affected the FC in the widely distributed regions. Importantly, all regions showed significant differences in FC between the central and eccentric directions, while few regions showed a difference between the left and right directions. These findings revealed the differences in the representations of sounds from different directions.

12.
J Ethnopharmacol ; 250: 111965, 2020 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-31185267

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Polycystic ovary syndrome (PCOS) is a complex gynecological endocrine disease commonly occurred in women of childbearing age. The main hallmark of PCOS includes elevated androgen production and insulin resistance (IR). Liuwei Dihuang Pills (LWDH Pills), a commonly prescribed traditional Chinese medicine (TCM) is widely used as a tonic prescription to treat diabetes, female menopause syndrome and other symptoms with'Kidney-Yin' deficiency. It has been reported the effects LWDH pills on PI3K/Akt signaling pathway in T2DM treatment. Recent studies have also indicated that the treatment of menopausal syndrome may be associated with the ovarian sexual hormone levels regulated by LWDH pills to alleviate female infertility. However, its potential benefits on PCOS have not been fully elucidated. AIM OF THE STUDY: The primary aim of this study was to investigate the alterations of PI3K/Akt pathway in polycystic ovary syndrome-insulin resistance (PCOS-IR) progression induced by letrozole combined with high fat diet (HFD) and then to explore the detailed mechanism of LWDH Pills to alleviate PCOS. MATERIALS AND METHODS: The female Sprague-Dawley rats were continuously treated with letrozole (p.o administration at 1 mg kg-1·day-1) and HFD for 21 days to establish the PCOS-IR model. Concurrently, metformin (200 mg kg-1·day-1) or LWDH Pills was orally administrated (1.2 or 3.6 g kg-1·day-1) to intervene disease progression. The ovarian pathology was evaluated by HE (hematoxylin-eosin) staining. The serum sexual hormones, follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol, testosterone, progesterone and fasting insulin (FINS) were determined by radioimmunoassay. The protein expressions of IRS-1, PI3Kp85α, Akt and FoxO1a were analyzed by western blotting, while the mRNA levels of follicle-stimulating hormone receptor (FSHR) and Cyp19a1 in ovarian tissue were measured by qPCR. RESULTS: The upregulated phosphorylation of IRS-1 (S307), down-regulated phosphorylation of PI3Kp85α, Akt, and FoxO1a were significantly reversed by LWDH Pills (3.6 g kg-1·day-1) in PCOS-IR rats with up-regulated mRNA levels of FSHR and Cyp19a1 in ovary. Also, the index of insulin resistance was gradually adjusted to normal by LWDH Pills. The serum levels of FSH, estradiol, progesterone levels were significantly raised while LH, testosterone were reduced. The ovarian polycystic changes were alleviated while the atresia follicles were reduced. CONCLUSION: LWDH Pills therapy obviously improved the ovarian polycystic pathogenesis and regained the development of follicles via upregulating Cyp19a1, alleviated insulin resistance through acting on PI3K/Akt signaling pathway. These findings have provided scientific evidence for LWDH Pills to treat PCOS.

13.
Brain Imaging Behav ; 14(1): 100-109, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30361945

RESUMO

2Sound decoding is important for patients with sensory loss, such as the blind. Previous studies on sound categorization were conducted by estimating brain activity using univariate analysis or voxel-wise multivariate decoding methods and suggested some regions were sensitive to auditory categories. It is proposed that feedback connections between brain areas may facilitate auditory object selection. Therefore, it is important to explore whether functional connectivity among regions can be used to decode sound category. In this study, we constructed whole-brain functional connectivity patterns when subjects perceived four different sound categories and combined them with multivariate pattern classification analysis for sound decoding. The categorical discriminative networks and regions were determined based on the weight maps. Results showed that a high accuracy in multi-category classification was obtained based on the whole-brain functional connectivity patterns and the results were verified by different preprocessing parameters. Insight into the category discriminative functional networks showed that contributive connections crossed the left and right brain, and ranged from primary regions to high-level cognitive regions, which provide new evidence for the distributed representation of auditory object. Further analysis of brain regions in the discriminative networks showed that superior temporal gyrus and Heschl's gyrus significantly contributed to discriminating sound categories. Together, the findings reveal that functional connectivity based multivariate classification method provides rich information for auditory category decoding. The successful decoding results implicate the interactive properties of the distributed brain areas in auditory sound representation.

14.
Arch Environ Contam Toxicol ; 78(2): 182-189, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31655873

RESUMO

This study investigated the levels, spatial distribution, sources, and ecological risks of 16 perfluorinated compounds (PFCs) in 68 surface soil samples (0-20 cm) from 7 cities in the Pearl River Delta (PRD), China. Sixteen target PFCs, including perfluoroalkyl carboxylic acids (PFCAs, C5-C14, C16, and C18) and perfluoroalkyl sulfonic acids (PFSAs, C4, C6, C8, and C10), were analyzed by high-performance liquid chromatography-negative electrospray ionization-tandem mass spectrometry (HPLC/ESI-MS/MS). Concentrations of total PFCs (∑PFCs) ranged from 2.19 to 98.5 µg kg-1 (dry weight, dw), with an average of 5.97 µg kg-1 dw. Perfluorooctane sulfonate (PFOS) was the dominant PFC, accounting for 23.9% of ∑PFCs. The highest ∑PFCs was found in the soil sample collected from Dongguan with a large number of manufacturing industries. There were no significant differences of ∑PFCs among unban, industrial, and agricultural soils, indicating similar pollution sources in soil of the PRD. More than 70% of ∑PFCs in soil of the PRD could be attributed to the four principal components, represented by PFOS and perfluorooctanoic acid (PFOA), perfluoropentanoic acid (PFPeA) and perfluorohexanoic acid (PFHxA), perfluorodecanoic acid (PFDA), and perfluoroundecanoic acid (PFUdA). Ecological risk assessment indicated that PFOA had low risk to soil plants and animals. However, the risk of PFOS to soil plants was relatively high in some studied regions.

15.
Biopreserv Biobank ; 18(1): 33-40, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31800305

RESUMO

Long-term cryopreservation of the viability and metabolic state of cells in cancer cell/tissue specimens has significant implications for diagnostic verification of disease progression in cancer patients and selection of effective treatment options via development of the patient-derived xenograft (PDX) models for drug screening. The purpose of this study is to investigate the effects of cryoprotectant agents (CPAs) on the expression of BCL-2 family genes (BCL-2, BAX, and BAD) that are involved in the growth and development of breast cancers. MCF-7 cells were cryopreserved in Dulbecco's modified Eagle's medium (DMEM) with 20% (v/v) fetal bovine serum, using 10% (v/v) Me2SO (dimethyl sulfoxide, DMSO) or 7.5% (v/v) Me2SO with 100is-300 mM trehalose as cryoprotectant solutions. After storage at -80°C for 7 days, the cells were thawed for evaluation. The use of Me2SO and trehalose has affected cell survival, proliferation, apoptotic state, as well as BCL-2 family gene expression. The conventional 10% (v/v) Me2SO method yields ∼80% post-thaw cell survival and good cell proliferation, but it drastically alters the pattern of the BCL-2 family gene expression. The antiapoptotic gene BCL-2 is downregulated, whereas two proapoptotic genes BAX and BAD are upregulated. The partial substitution of Me2SO with 200 or 300 mM trehalose enhances cell proliferation of survived cells after cryopreservation. The presence of trehalose upregulates the expression of both the antiapoptotic gene BCL-2 and proapoptotic genes BAX and BAD. Cryopreservation could tip off the checkpoint of the apoptotic pathway regulated by the BCL-2 family members, and the effect may be protectant dependent. The findings of this study demonstrate the importance of paying attention to the potential change of gene expression and metabolic state of cancer cells after cryopreservation in an attempt to development of the PDX models from cryopreserved cancer cells or tissue specimens.

16.
Int J Food Microbiol ; 312: 108358, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31655356

RESUMO

Meat products are commonly regarded as one of the main sources of human listeriosis caused by Listeria monocytogenes. The objective of this study was to estimate the prevalence of L. monocytogenes in a range of meat products from 24 different Chinese regions by using meta-analysis of literature data and a novel sensitivity analysis approach. A total of 112 publications from five databases, published between 1 January 2007 and 31 December 2017, were systematically selected for relevance and covered meat products sampled between 2000 and 2016. Estimated by the random-effects model, the pooled prevalence of L. monocytogenes was 8.5% (95% CI: 7.1%-10.3%) in raw meats and 3.2% (95% CI: 2.7%-3.9%) in ready-to-eat (RTE) meats. The prevalence differed from high to low among raw meats including prefabricated raw meats 12.6% (95% CI: 6.9%-21.7%), fresh pork 11.4% (95% CI: 8.6%-14.9%), fresh beef 9.1% (95% CI: 6.3%-13.0%), fresh poultry 7.2% (95% CI:4.9%-10.4%), frozen raw meats 7.2% (95% CI: 5.7%-9.0%), and fresh mutton 5.4% (95% CI: 2.5%-11.0%). A higher L. monocytogenes prevalence level was shown in the meat products from central and northeastern China provincial regions. The entropy-based sensitivity analysis utilized in the meta-analysis indicated that the sampling period and location were two critical factors influencing the prevalence level of L. monocytogenes in meat products. A better understanding of differences in prevalence levels per geographic region and between meat product sources may allow the competent authorities, industry, and other relevant stakeholders to tailor their interventions to control the occurrence of L. monocytogenes in meat products effectively.


Assuntos
Contaminação de Alimentos/análise , Microbiologia de Alimentos , Listeria monocytogenes/isolamento & purificação , Produtos da Carne/microbiologia , Aves Domésticas/microbiologia , Animais , Bovinos , China , Humanos , Listeriose/epidemiologia , Prevalência , Ovinos , Suínos
17.
Front Oncol ; 9: 1233, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31799193

RESUMO

Objective: To investigate Livin-mediated regulation of H2A.XY142 phosphorylation via a novel kinase activity and its effect on autophagy in colon cancer cells. Methods: The interaction between Livin and H2A.X was tested by immunoprecipitation. H2A.X-/- HCT116 cells were transfected with human influenza hemagglutinin (HA)-tagged WT or Y142F phospho-dead mutantH2A.X plasmids. GST-tagged recombinant Livin protein was used to perform in vitro pull-down experiment and kinase assay. H2A.X-/-Livin+/+ SW480 cells were co-transfected with H2A.XWT/H2A.XY142F plasmid and LC3 EGFP-tagged plasmid to explore whether H2A.XY142F was involved in Livin-mediated autophagy induced by starvation in colon cancer cells. Results: Co-immunoprecipitation studies confirmed that Livin interacted with H2A.X and that it was phosphorylation dependent. In vitro kinase assay confirmed that Livin could phosphorylate H2A.X. Knockdown of Livin (Livin-/-) in SW480 cells or HCT116 cells canceled the starvation-induced autophagy in colon cancer cells; H2A.X-/-Livin+/+ SW480 cells transfected with H2A.XWT activated autophagy induced by starvation while cells transfected with H2A.XY142F had no significant difference; Livin-H2A.XY142F axis activated autophagy in colon cancer cells through transcriptionally regulating ATG5 and ATG7. Conclusion: Livin promotes autophagy in colon cancer cells via regulating the phosphorylation of H2A.XY142.

18.
Front Neurosci ; 13: 1111, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31736683

RESUMO

Emotions can be perceived from both facial and bodily expressions. Our previous study has found the successful decoding of facial expressions based on the functional connectivity (FC) patterns. However, the role of the FC patterns in the recognition of bodily expressions remained unclear, and no neuroimaging studies have adequately addressed the question of whether emotions perceiving from facial and bodily expressions are processed rely upon common or different neural networks. To address this, the present study collected functional magnetic resonance imaging (fMRI) data from a block design experiment with facial and bodily expression videos as stimuli (three emotions: anger, fear, and joy), and conducted multivariate pattern classification analysis based on the estimated FC patterns. We found that in addition to the facial expressions, bodily expressions could also be successfully decoded based on the large-scale FC patterns. The emotion classification accuracies for the facial expressions were higher than that for the bodily expressions. Further contributive FC analysis showed that emotion-discriminative networks were widely distributed in both hemispheres, containing regions that ranged from primary visual areas to higher-level cognitive areas. Moreover, for a particular emotion, discriminative FCs for facial and bodily expressions were distinct. Together, our findings highlight the key role of the FC patterns in the emotion processing, indicating how large-scale FC patterns reconfigure in processing of facial and bodily expressions, and suggest the distributed neural representation for the emotion recognition. Furthermore, our results also suggest that the human brain employs separate network representations for facial and bodily expressions of the same emotions. This study provides new evidence for the network representations for emotion perception and may further our understanding of the potential mechanisms underlying body language emotion recognition.

19.
J Food Prot ; 82(11): 1820-1827, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31596616

RESUMO

This study aimed to estimate the growth parameters of Listeria monocytogenes growth in ready-to-eat (RTE) braised beef by one-step dynamic and static kinetic analysis. The Baranyi model and cardinal parameters model were integrated into a dynamic and static model to estimate the kinetic parameters under one dynamic condition (-20 to 40.0°C) and eight static conditions (4, 8, 15, 20, 30, 35, 37, and 40°C). Based on the dynamic and static methods, the respective dynamic and static results for estimated growth boundaries of L. monocytogenes in RTE braised beef were from -2.5 and -2.7°C to 40.5 and 40.7°C with optimal specific growth rates of 1.078 and 0.913 per h at temperatures of 35.7 and 35.0°C. Temperature effects on the specific growth rate and lag period were developed and used to simulate the change of the physiological state of inocula during the bacterial growth. Subsequently, three additional dynamic temperature profiles were implemented for external validation. The root mean square error of the model developed by dynamic regression (0.19 log CFU/g) is slightly better than that of the model developed by static regression (0.23 log CFU/g). Comparing the validation results, one-step dynamic analysis might be a preferable method for prediction, especially when the growth approaches the stationary phase. Generally, both one-step dynamic and static analyses could be used to accurately predict L. monocytogenes growth in RTE braised beef under fluctuating temperatures.

20.
Chin Med Sci J ; 34(3): 205-210, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31648673

RESUMO

Objective To clarify the possible association of GSTT1 homozygous deletion with the susceptibility to pancreatic cancer. Methods We searched PubMed database, Chinese Journal Full Text Database (CNKI), and EMBASE to find the eligible studies published up to April 18, 2018 for evaluating the relationship between GSTT1 homozygous deletion and pancreatic cancer. The frequency of null genotype for GSTT1 between the pancreatic cancer group and the healthy control group was compared with Chi-square test, and odds ratios (ORs) value and 95% confidence interval (95% CI) were calculated. Results A total of 9 studies met the inclusion criteria, and 5952 cases consisting of 2387 pancreatic cancer patients and 3565 healthy controls were included in the meta analysis. Compared with the control group, frequency of null genotype for GSTT1 in the pancreatic cancer group was higher (33.4% vs. 38.7%, OR = 1.26, 95%CI = 1.01-1.58, P = 0.04). Conclusion GSTT1 homozygous deletion individuals may have higher susceptibility to pancreatic cancer.

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