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1.
Adv Healthc Mater ; : e2000046, 2020 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-32400080

RESUMO

Biomimetic mineralization of live organisms shows extraordinary promise in biotechnology. However, their therapeutic applications have been insufficiently explored. Herein, it is demonstrated that metal-organic framework (MOF)-engineered bacteria are powerful carriers for tumor-targeted therapeutic delivery. Specifically, Escherichia coli (MG1655) is coated with a zeolitic imidazolate framework-8 layer coloaded with a photosensitizer and chemical drug through a one-step in situ method. The as-prepared bacteria@MOF hybrid preserves its viability and tumor selectivity. It exhibits high therapeutic efficacy both in vitro and in vivo in a combined chemo-photodynamic manner. To the best of knowledge, this is the first report of engineered bacteria@MOFs for in vivo tumor treatment. This study opens a new horizon for the bioapplications of biomineralized organisms and may provide novel strategies against tumors.

2.
J Pharm Sci ; 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-32240697

RESUMO

Different solid forms possess various physicochemical properties, which can significantly affect the stability, bioavailability, and manufacturability of the final product. DP-VPA, a complex of 1-stearoyl-2-valproyl-sn-glycero-3-phosphatidylcholine (DP-VPA-C18) and 1-palmitoyl-2-valproyl-sn-glycero-3-phosphatidylcholine (DP-VPA-C16), is currently under development as an antiepileptic drug. DP-VPA-C16 and DP-VPA-C18 crystallize together in solid solution forms. The solid forms of DP-VPA solid solution were studied herein. Powder X-ray diffraction (PXRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM), dynamic vapor sorption (DVS) and optical microscopy were used to characterize the different crystalline forms, known as polymorphs. The physicochemical properties, including hygroscopicity, thermodynamic behavior, and relative stability, of each form were investigated. DVS analysis showed that DP-VPA solid solution reduced the hygroscopicity of DP-VPA-C16. The relative humidity stability study revealed that Forms A and B are relatively stable, while Forms A-1, B-1, C and D are highly unstable under natural humidity. Further analysis revealed that Form A transforms into Form B through milling. Given the physicochemical properties of the available physical forms, Form B may be the optimal form for the formulation and development of antiepileptic drugs.

3.
Talanta ; 215: 120900, 2020 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-32312445

RESUMO

During the past decades, few micro-devices for analysis of associative learning behavior have been reported. In this work, an agarose-PDMS hybridized micro-chip was developed to establish a new associative learning model between mechanosensation and food reward in C. elegans. The micro-chip consisted of column arrays which mimicked mechanical stimulation to C. elegans. After trained by pairing bacterial food and mechanical stimuli in the chip, the worms exhibited associative learning behavior and gathered in the regions where there was food during training. The key research findings include: (1) Associative learning behavior of C. elegans could be generated and quantitatively analyzed by this developed micro-chip. (2) Associative learning behavior could be enhanced by extending the training time and developmental stage. (3) Mechanosensation-related genes and neurotransmitters signals had effects on the learning behavior. (4) The associative learning ability could be strengthened by exogenous dopamine in both wild type and mutants. We validated that the design of the micro-chip was useful and convenient for the study of learning behavior based on mechanosensation.

4.
Sci Rep ; 10(1): 4596, 2020 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-32165719

RESUMO

The three-dimensional hierarchical morphology of surfaces greatly affects the wettability, absorption and microfabrication properties of their hybrid materials, however few scalable methods exist that controls simultaneously complex geometric shape and spatial scattered location and their physical properties tuned. Consequently, this report describes a synthetic strategy that enables the position of well-ordered biomorph nano-microstructures on hydrophobic surfaces to be precisely controlled. The hierarchical architecture can be accurately positioned on polydimethylsiloxane (PDMS) surfaces in an unprecedented level by leveraging a solid/liquid/gas triphase dynamic reaction diffusion system strategy. The effect of salt concentrations, pH, CO2 levels, temperature and substrate patterning on this self-assembly process has been investigated, enabling protocols to be devised that enables the hydrophobic properties of the hierarchically assembled multiscale microstructures to be tuned as required. This combined top-down/bottom-up approach can be used to produce composites with outstanding hydrophobicity properties, affording superhydrophobic materials that are capable of retaining water droplets on their surfaces, even when the material is inverted by 180°, with a wide range of potential applications in oil/water separation technology and for selective cell recognition in biological systems.

5.
Analyst ; 145(8): 3136-3147, 2020 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-32186558

RESUMO

Colorectal cancer is associated with changed IgG glycosylation, but the alteration in specific subclasses of IgG is unknown. Initially, we optimized five common IgG glycopeptide enrichment methods to acquire a comprehensive profile of IgG glycopeptides. However, an incomplete tryptic digestion of IgG occurred when using an ordinary protease to protein ratio, which significantly impacted the final statistical analysis. Herein, we introduced a two-step enzymatic digestion, enabling the complete digestion of IgG glycopeptides and further improving the detection intensity of the target glycopeptides. In order to rapidly process and automatically integrate the MS data, we developed a simple and effective code using MATLAB. Following statistical analysis, we observed that IgG1_H3N4F1 and IgG1_H3N4 were substantially increased in CRC, while IgG1_H5N5F1, IgG1_H5N4F1S1 and IgG2_H5N4F1 were markedly decreased. A further evaluation of the diagnostic performance showed that they all achieved a fair performance in discriminating the patients from the normal. In terms of the glycan features, it was demonstrated that the CRC progression was associated with increased agalactosylation, and the decreased digalactosylation and galactosylation per antenna on the diantenna glycans of IgG1 and IgG2. Concurrently, the decreased sialylation of IgG1 was strongly correlated with CRC. Moreover, an analysis of tumor-specific glycosylation showed that the alterations of IgG glycosylation were more significant in colon cancer, and no obvious difference was observed between colon and rectal cancer. This study comprehensively optimized the glycopeptide enrichment methods, evaluated the enzymatic digestion effect, and explored the association between CRC progression and subclass-specific glycosylation.

6.
ACS Appl Mater Interfaces ; 12(10): 11329-11340, 2020 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-32072808

RESUMO

Designing a multifunctional theranostic nanoplatform with optional therapeutic strategies is highly desirable to select the most suitable therapeutic manners for the patient's cancer treatment. Among all shapes of silver materials, a silver nanoprism was reported to have great potential in photothermal therapy (PTT) owing to its strong surface plasmon resonance band in the near-infrared region. However, its instability in physicochemical environments and its severe toxicity confined its further application. To overcome this, herein, we demonstrated a silver prism-polydopamine (PDA) hybrid nanoplatform for tumor treatment with three therapeutic strategies. Specifically, the PDA coating endows the silver prism with excellent stability, high photothermal conversion, long-term in vivo biocompatibility, ease of decorating targeting ligands, and drug delivery. Upon near-infrared laser irradiation (808 nm, 1 W/cm2), tumors can be eradicated by the as-prepared nanoparticle through monomodal PTT. Besides, when combined with a chemical drug, this nanoparticle is able to inhibit tumor growth via combined photochemotherapy under a lower laser treatment (0.7 W/cm2). Furthermore, by supplementing with an immune checkpoint blockade, the realized synergistic photochemoimmunotherapy exhibits high efficacy to inhibit tumor relapse and metastasis. Moreover, owing to the high photothermal conversion efficiency and great X-ray attenuation ability of the silver nanoprism, our designed nanoplatform can be used in photoacoustic, computed tomography, and infrared thermal multimodal imaging. Our study provides a multifunctional nanoparticle for tumor theranostics, and this therapeutic strategy-optional nanoplatform shows promise in future biomedicine.

7.
J Chromatogr A ; 1619: 460934, 2020 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-32029268

RESUMO

Peptide-N-glycosidase F (PNGase F) is the most frequently used enzyme to release N-glycan from glycoproteins in glycomics; however, the releasing process using PNGase F is tedious and can range in duration from hours to overnight. Recently, efforts have been made to accelerate this enzymatic reaction, and they include the use of microwave irradiation, ultrahigh pressure, enzyme immobilization, and other techniques. Here, we developed a novel method combining the oriented immobilization of PNGase F on magnetic particles and microwave-assisted enzymatic digestion techniques to achieve highly efficient release of N-glycans. The oriented immobilization of PNGase F on magnetic particles utilizes the affinity of its co-expressed His-tag towards iminodiacetic acid-Nickel modified magnetic particles. Compared with non-oriented immobilization, the oriented immobilization of PNGase F exhibits several advantages including tolerance to high temperature (52 °C) and the ability to retain strong activity after more than five reuses. When used in combination with microwave irradiation, efficient N-glycan removal from ribonuclease B was achieved within 5 min. The proposed strategy was also used to release glycan from fetuin and human serum and has proven to provide a promising deglycosylation method for the characterization of protein glycosylation.

8.
Eur J Med Chem ; 191: 112144, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32087465

RESUMO

We designed and synthesized a novel series of piperidine propionamide derivatives as potent sigma-1 (σ1) receptor antagonists and mu (µ) opioid receptor agonists, and measured their affinity for σ1 and µ receptors in vitro through binding assays. The basic scaffold of the new compounds contained a 4-substituted piperidine ring and N-aryl propionamide. Compound 44, N-(2-(4-(4-fluorobenzyl) piperidin-1-yl) ethyl)-N-(4-methoxy-phenyl) propionamide, showed the highest affinity for σ1 receptor (Ki σ1 = 1.86 nM) and µ receptor (Ki µ = 2.1 nM). It exhibited potent analgesic activity in the formalin test (ED50 = 15.1 ± 1.67 mg/kg) and had equivalent analgesic effects to S1RA (σ1 antagonist) in a CCI model. Therefore, Compound 44, which has mixed σ1/µ receptor profiles, may be a potential candidate for treating neuropathic pain.

9.
J Sep Sci ; 43(1): 258-270, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31654552

RESUMO

Microfluidic chip electrophoresis has been widely employed for separation of various biochemical species owing to its advantages of low sample consumption, low cost, fast analysis, high throughput, and integration capability. In this article, we reviewed the development of four different modes of microfluidics-based electrophoresis technologies including capillary electrophoresis, gel electrophoresis, dielectrophoresis, and field (electric) flow fractionation. Coupling detection schemes on microfluidic electrophoresis platform were also reviewed such as optical, electrochemical, and mass spectrometry method. We further discussed the innovative applications of microfluidic electrophoresis for biomacromolecules (nucleic acids and proteins), biochemical small molecules (amino acids, metabolites, ions, etc.), and bioparticles (cells and pathogens) analysis. The future direction of microfluidic chip electrophoresis was predicted.

10.
Biochim Biophys Acta Gen Subj ; 1864(3): 129510, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31887339

RESUMO

BACKGROUD: Given the increasing morbidity and mortality of colorectal cancer (CRC), it is urgent to develop a noninvasive screening strategy for early diagnosis of CRC. Altered IgG glycosylation is associated with CRC progression, whereas the association of IgG isomeric glycosylation with CRC were not investigated. METHODS: Methylamidation of IgG N-glycans was conducted prior to PGC-based nanoLC-ESI-MS/MS analysis. Data processing was operated by a self-developed application based on MATLAB solution. Statistical analysis including K-S test, t-test, ROC curve and OPLS-DA were successively performed. Additionally, an independent set was utilized to validate the results. RESULTS: Total 28 IgG glycans and 79 compositional isomers were identified, over half of which are firstly identified so far. Statistical analysis showed that CRC associates with increase in IgG agalactosylation, decrease in IgG sialylation and fucosylation of sialylated glycans. Additionally, it was found that three compositional isomers (H3N4F1-a, H3N4F1-b and H4N3S1F1-e) could distinguish CRC and early stages from controls with an accurate area under the receiver operating characteristic curve. Significantly, these results were validated in an independent set by multivariate statistical analysis. CONCLUSIONS: This is the first comprehensively profiling of isomer-specific IgG N-glycosylation, which could differentiate normal controls from colorectal disease patients. The candidate IgG glyco-biomarkers provide important screening indicators for early diagnosis of CRC. GENERAL SIGNIFICANCE: Colorectal cancer progression is strongly associated with isomer-specific IgG N-glycosylation.

11.
Talanta ; 208: 120484, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31816727

RESUMO

Gas embolism is the abnormal emergence of bubble in the vascular system, which can induce local ischemic symptoms. For studying the mechanism underlying gas embolism and revealing local ischemic diseases information, novel technique for analyzing cells response to bubble contact with high controllability is highly desired. In this paper, we present an integrated microfluidic device for the precise generation and control of microbubble based on the gas permeability of polydimethysiloxane (PDMS) to study the effect of bubble's mechanical contact on cells. Cell viability analysis demonstrated that short-term (<15 min) bubble contact was generally non-lethal to cultured endothelial cells. The significant increase in intracellular calcium of the microbubble-contacted cells and cell-to-cell propagation of calcium signal in the adjacent cells were observed during the process of bubble expansion. In addition, the analysis of intercellular calcium signal in the cells treated with suramin and octanol revealed that cell-released small nucleotides and gap junction played an important role in regulating the propagation of calcium wave triggered by bubble contact. Thus, our microfluidic method provides an effective platform for studying the effect of gas embolism on cultured adherent cells and can be further needed for anti-embolism drugs test.

12.
Adv Mater ; 31(46): e1905825, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31566283

RESUMO

Synergistic phototherapy has the potential to conquer the extreme heterogeneity and complexity of difficult tumors and result in better cancer treatment outcomes than monomodal photodynamic therapy (PDT) or photothermal therapy (PTT). However, the previous approaches to combining PDT and PTT are mainly focused on primary tumor obliteration while neglecting tumor metastasis, which is responsible for about 90% of cancer deaths. It is shown that a combined PDT/PTT approach, based on upconversion-polymer hybrid nanoparticles with surface-loaded chlorin e6 photosensitizer, can enhance primary tumor elimination and elicit antitumor immunity against disseminated tumors. The specifical arrangement of an external upconversion coating over the polymer core ensures adequate photoabsorption by the upconversion nanoparticles for the generation of reactive oxygen species upon single near-infrared light irradiation. Furthermore, it is found that synergistic phototherapy can elicit robust systemic and humoral antitumor immune responses. When combined with immune checkpoint blockades, it can inhibit tumor relapse and metastasis as well as prolong the survival of tumor-bearing mice in two types of tumor metastasis models. This study may establish a new modality for enhancing immunogenic cell death through a synergistic phototherapeutic nanoplatform and extend this strategy to overcome tumor metastasis with an augmented antitumor immune response.

13.
Nat Commun ; 10(1): 4087, 2019 09 09.
Artigo em Inglês | MEDLINE | ID: mdl-31501430

RESUMO

Untethered small actuators have various applications in multiple fields. However, existing small-scale actuators are very limited in their intractability with their surroundings, respond to only a single type of stimulus and are unable to achieve programmable structural changes under different stimuli. Here, we present a multiresponsive patternable actuator that can respond to humidity, temperature and light, via programmable structural changes. This capability is uniquely achieved by a fast and facile method that was used to fabricate a smart actuator with precise patterning on a graphene oxide film by hydrogel microstamping. The programmable actuator can mimic the claw of a hawk to grab a block, crawl like an inchworm, and twine around and grab the rachis of a flower based on their geometry. Similar to the large- and small-scale robots that are used to study locomotion mechanics, these small-scale actuators can be employed to study movement and biological and living organisms.


Assuntos
Biomimética/instrumentação , Grafite/química , Polímeros/química , Pirróis/química , Robótica
14.
Langmuir ; 35(34): 11123-11131, 2019 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-31369286

RESUMO

Three-dimensional porous material holds enormous potential in the field of life science and environmental protection. In this work, we proposed a facile route for the large-scale synthesis of porous poly(dimethylsiloxane) (PDMS) sponge via paraffin oil based emulsion technique. A stable emulsion could be formed by emulsifying water in the PDMS solution with the aid of paraffin oil. Moreover, the amount of emulsified water in 5 g of PDMS solution is as high as 35 g, and the skeleton of the prepared PDMS sponge is still in intact. This method is cost-effective, rapid, and easily scaled up. The water contact angle of the obtained PDMS sponge is 141.9 ± 1°, and the absorption capacities of the sponges are 13.5-33.3 g g-1 for various organic solvents. The PDMS sponge only needed 0.069 MPa force to realize the compression ratio of 90%, which it still maintained after over 50 cycles of compression. In addition, the porous PDMS sponge exhibited an excellent oil absorption and an outstanding reusability, which were potentially useful in water purification.

15.
Talanta ; 205: 120136, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31450434

RESUMO

Investigating the kinetics of biochemical reactions plays vital roles in understanding the reaction mechanism. However, many biochemical reactions occur in the time scale of millisecond or even microsecond, which is out of the time-resolution limit of traditional methods. Microfluidic mixers, also referred to micromixers, have been widely developed to resolve the kinetics of biochemical reactions, due to their short mixing time (down to microsecond scale), low sample consumption (as low as several microliters), capability of mixing high-viscosity fluids (up to 35.25 mPa s) and ease of integration with different detection techniques. In this review, we briefly introduced mixing mechanism in micromixers, and discussed the mixing principle of four main types of micromixers: hydrodynamic focusing micromixer, chaotic convection micromixer, droplet micromixer and acoustic micromixer. We then presented how these micromixers applied in investigating enzymatic constant, resolving the kinetics of biomolecule folding or studying biomolecular interactions. At last we summarized the detection technologies used in micromixers for measuring the kinetics and predicted the potential future development on micromixers. We anticipated that micromixers would attract more attentions and would advance the field of kinetics study of biochemical reactions in future.

16.
J Chromatogr A ; 1600: 105-111, 2019 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-31056268

RESUMO

Efficient sample pretreatment of N-glycans from glycoproteins is essential but challenging due to the limitations of existing tedious and laborious methods in N-glycomics. This study aimed to establish a filter-aided extraction method coupled with glycosylamine AQC labeling for a simple and rapid direct HPLC-FLD-based analysis of N-glycans. The developed method was demonstrated to be simpler and more sensitive compared to previous HILIC SPE purification method coupled with glycosylamine labeling. It has been validated with wild-type N-glycans from human transferrin and RNase B and then was successfully applied to investigate N-glycan profiles of the transferrin in human serum and a monoclonal antibody (mAb). Results showed good applicability of the method for complex samples. Additionally, this method is compatible with the replicate determination of N-glycan samples to assess the high-throughput analysis of glycan variability in mAb sample.


Assuntos
Cromatografia Líquida de Alta Pressão , Glicômica/métodos , Polissacarídeos/análise , Filtração , Glicômica/instrumentação , Glicoproteínas , Glicosilação , Humanos , Reprodutibilidade dos Testes , Transferrina/análise
17.
Sci Rep ; 9(1): 4865, 2019 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-30890747

RESUMO

Enzyme immobilization is widely applied in biocatalysis to improve stability and facilitate recovery and reuse of enzymes. However, high cost of supporting materials and laborious immobilization procedures has limited its industrial application and commercialization. In this study, we report a novel self-assembly immobilization system using bacteriophage T4 capsid as a nanocarrier. The system utilizes the binding sites of the small outer capsid protein, Soc, on the T4 capsid. Enzymes as Soc fusions constructed with regular molecular cloning technology expressed at the appropriate time during phage assembly and self-assembled onto the capsids. The proof of principle experiment was carried out by immobilizing ß-galactosidase, and the system was successfully applied to the immobilization of an important glycomics enzyme, Peptide-N-Glycosidase F. Production of Peptide-N-Glycosidase F and simultaneous immobilization was finished within seven hours. Characterizations of the immobilized Peptide-N-Glycosidase F indicated high retention of activity and well reserved deglycosylation capacity. The immobilized Peptide-N-Glycosidase F was easily recycled by centrifugation and exhibited good stability that sustained five repeated uses. This novel system uses the self-amplified T4 capsid as the nanoparticle-type of supporting material, and operates with a self-assembly procedure, making it a simple and low-cost enzyme immobilization technology with promising application potentials.

18.
Anal Sci ; 35(6): 609-618, 2019 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-30853696

RESUMO

A microfluidic device as a pivotal research tool in chemistry and life science is now widely recognized. Indeed, microfluidic techniques have made significant advancements in fundamental research, such as the inherent heterogeneity of single-cells studies in cell populations, which would be helpful in understanding cellular molecular mechanisms and clinical diagnosis of major diseases. Single-cell analyses on microdevices have shown great potential for precise fluid control, cell manipulation, and signal output with rapid and high throughput. Moreover, miniaturized devices also have open functions such as integrating with traditional detection methods, for example, optical, electrochemical or mass spectrometry for single-cell analysis. In this review, we summarized recent advances of single-cell analysis based on various microfluidic approaches from different dimensions, such as in vitro, ex vivo, and in vivo analysis of single cells.


Assuntos
Dispositivos Lab-On-A-Chip , Análise de Célula Única/instrumentação , Animais , Humanos
19.
Nanoscale ; 11(10): 4562-4570, 2019 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-30806402

RESUMO

Self-propelled micro/nanomotors play a crucial role in the fields of biomedicine, energy and the environment but are limited by low throughput and a tedious fabrication approach. Here, we propose a simple microfluidics-based scheme for fabricating substrate-free graphene oxide (GO)-based micromotors of different shapes and sizes with high throughput. The micromotors are designed to possess a 'Janus'-like porous structure, and half of each micromotor is modified with hierarchical Pt nanoflowers, which can promote the wetting of Pt with an H2O2 solution and result in a high speed of movement. To investigate the applicability of the micromotors, they were employed to rapidly remove an antibiotic, namely, tetracycline, from a solution. It was found that the rapid movement of the micromotors increased the mass transfer of tetracycline and the frequency of collisions between tetracycline molecules and the micromotors, which led to a high removal efficiency. The direction of movement of the micromotors can be conveniently controlled by an external magnetic field. Furthermore, the removal efficiency and removal time as functions of the number of micromotors, the adsorption kinetics and adsorption isotherm, and the removal amount as a function of the pH were investigated. This proved that the micromotors that were constructed exhibit high adsorption capabilities for tetracycline and implied that they hold great promise for the removal of antibiotics with similar structures or other pollutants, including organic compounds, heavy metals and oil droplets.


Assuntos
Antibacterianos/química , Grafite/química , Peróxido de Hidrogênio/química , Campos Magnéticos , Platina/química , Concentração de Íons de Hidrogênio , Cinética , Porosidade
20.
Lab Chip ; 19(3): 475-483, 2019 01 29.
Artigo em Inglês | MEDLINE | ID: mdl-30601555

RESUMO

The directed motility of organisms in response to fluid velocity, which is called rheotaxis, is important in the life cycle of C. elegans, enabling them to navigate their environment and maintain their positions in the presence of adverse flow. Thus, to study the mechanism underlying rheotaxis behavior and reveal information on parasitic diseases, the profile analysis of the rheotaxis response in worm populations with high resolution in well-defined fluid environments is highly desirable. In this work, we presented a rapid and robust microfluidic approach to quantitatively analyze the rheotaxis behavior of worms in response to velocity. The flow-based microfluidic chip contained six helical spline microchannels for generating six flow streams with different flow velocities. Since the worms loaded in the chip would swim upstream into channels, the distribution of the worms in response to the different flow velocities was successfully monitored for the quantitative analysis of their rheotaxis behavior using this microfluidic chip. The results indicated that the rate range of around 50 µm s-1 was the most favorable flow velocity for the wild-type worms. Further, we analyzed ASH neuron-blocked worms and found that the functionally defective ASH neurons inhibited their sensitivity to flow rate. In addition, the rheotaxis analysis of the mutant worms indicated that TRP mechanosensory channels and serotonin signals also play a regulatory role in the rheotaxis response of these worms. Thus, our microfluidic method provides a useful platform to study the rheotaxis behaviors in C. elegans and can be further applied for anti-parasitic drug tests.


Assuntos
Caenorhabditis elegans/fisiologia , Técnicas Analíticas Microfluídicas , Movimento , Reologia/instrumentação , Animais , Fenômenos Biomecânicos , Caenorhabditis elegans/genética , Caenorhabditis elegans/metabolismo , Desenho de Equipamento , Mutação , Neurotransmissores/metabolismo
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