Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 12 de 12
Filtrar
Mais filtros










Base de dados
Tipo de estudo
Intervalo de ano de publicação
1.
Mediators Inflamm ; 2019: 8247019, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31827381

RESUMO

Background: High levels of branched-chain amino acids (BCAAs) and aromatic amino acids (AAAs) were associated with an increased risk of hyperglycemia and the onset of diabetes. This study is aimed at assessing circulating valine concentrations in subjects with type 2 diabetes (T2D) and in T2D patients and high-fat diet- (HFD-) fed mice treated with the hypoglycemic agent sitagliptin (Sit) and analyzing the association of valine concentrations with metabolic parameters. Methods: Metabolomics in HFD-fed mice were analyzed by gas chromatography-mass spectrometry (GC-MS) systems. Plasma valine concentrations were detected with a commercial kit in 53 subjects with normal glucose levels (n = 19), newly diagnosed T2D (n = 20), placebo-treated T2D (n = 7), or Sit-treated T2D (n = 7). Biochemical parameters were also assessed in all participants. Results: Sit treatment markedly changed the pattern of amino acid in HFD-fed mice, especially by reducing the level of the BCAA valine. Compared with the healthy controls, the plasma valine concentrations were significantly higher in the T2D patients (p < 0.05). Correlation analysis showed that the plasma valine concentration was positively correlated with the level of fasting plasma glucose (p < 0.05). Moreover, the plasma valine concentrations were notably reduced after Sit treatment in T2D patients (p < 0.05). Conclusions: Our findings demonstrate an important effect of Sit on the BCAA valine in T2D patients and HFD-fed mice, revealing a new hypoglycemic mechanism of it. Furthermore, the results suggest that the circulating valine level might be a novel biomarker for T2D and restoring the level of valine might be a potential strategy for diabetes therapy.

2.
Nat Commun ; 10(1): 5013, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31676774

RESUMO

For atomically thin two-dimensional materials, interfacial effects may dominate the entire response of devices, because most of the atoms are in the interface/surface. Graphene/sapphire has great application in electronic devices and semiconductor thin-film growth, but the nature of this interface is largely unknown. Here we find that the sapphire surface has a strong interaction with some of the carbon atoms in graphene to form a C-O-Al configuration, indicating that the interface interaction is no longer a simple van der Waals interaction. In addition, the structural relaxation of sapphire near the interface is significantly suppressed and very different from that of a bare sapphire surface. Such an interfacial C-O-Al bond is formed during graphene growth at high temperature. Our study provides valuable insights into understanding the electronic structures of graphene on sapphire and remote control of epitaxy growth of thin films by using a graphene-sapphire substrate.

3.
J Mater Sci Mater Med ; 30(11): 125, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31728639

RESUMO

OBJECTIVE: To evaluate the clinical outcomes of reconstruction of maxillary class III defect using 3D-printed titanium mesh. METHODS: Twelve patients with maxillary class III defect from April 2015 to December 2016 were retrospectively studied. A 3D individualized maxillary stereo model based on mirror images of the unaffected maxilla was obtained to fabricate an anatomically adapted titanium mesh using computer-assisted design and manufacture. The individual titanium mesh was inserted into the maxillary class III defect after total maxillectomy. The incidence of postoperative complications was evaluated. The postoperative orbital volume and protrusion degree of eye were measured. RESULTS: All patients were satisfied with their postoperative facial symmetry, without developing diplopia or endophthalmos. The postoperative orbital volumes were 26.41 ± 0.52 mL on the affected side and 26.55 ± 0.45 mL on the unaffected side. The postoperative protrusion degrees of affected and unaffected eyes were 16.21 ± 0.48 and 16.82 ± 0.79 mm, respectively. Titanium mesh exposure was observed in 2 patients and mild limitation of mouth opening was observed in 4 patients who underwent postoperative radiotherapy. CONCLUSION: Reconstruction of maxillary class III defect with 3D-printed titanium mesh can achieve successful clinical outcomes, which recovered orbital volume and protrusion degree of eye. Twelve patients with maxillary class III defect were satisfied with their postoperative facial symmetry, without developing diplopia or endophthalmos. We investigated that reconstruction of maxillary class III defect with 3D-printed titanium mesh can achieve successful clinical outcomes.

4.
Int J Biochem Cell Biol ; 115: 105578, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31374250

RESUMO

Odontoblasts forms dentin as the main hard tissue of the tooth. Autophagy is a highly conserved homeostasis involved in odontoblast differentiation. DSPP (Dentin sialophosphoprotein) and Cbfα1 (Core binding factor α1) are typical factors in odontoblasts of the dentin-pulp complex. However, the relationship between Cbfα1 and DSPP as well as the role of Cbfα1 involved in the autophagy during odontoblast differentiation are poorly understood. In this study, we found the stimulation with FGF2 in MDPC-23 cells resulted in the suppression of autophagy and promotion of cell differentiation. We demonstrated that both mRNA and protein levels of Cbfα1 and DSPP were elevated upon FGF2 stimulation coupled with the inhibition of autophagy in MDPC-23 cells. Further results revealed that overdose of Cbfα1 increased the expression of DSPP, and also suppressed autophagy in MDPC-23 cells. Using luciferase reporter assay and EMSA, we illustrated that Cbfα1 upregulated DSPP expression through binding to the specific sites 5'-TACCTCA (-3950 bp to -3944 bp) and 5'-ACCACA (-3106 bp to -3101 bp) in DSPP promoter. Furthermore, ChIP assay results revealed that FGF2 treatment enhanced this binding of Cbfα1 to DSPP promoter. Signaling pathway inhibitor screening further revealed Cbfα1 suppressed autophagy involved in Wnt I signaling. Moreover, knockdown of Cbfα1 using siRNA silencing approach reversed the FGF2-mediated suppression of autophagy in MDPC-23 cells. Thus, these findings illustrate a novel regulatory mechanism by which Cbfα1 transcriptionally enhances DSPP expression, and also provide an insight into the inhibitory action of Cbfα1 in autophagy thereby facilitating odontoblast differentiation upon FGF2 stimulation.

5.
Oncol Lett ; 18(1): 667-673, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31289540

RESUMO

Effect of microRNA-27b (miR-27b) on cisplatin chemotherapy sensitivity of oral squamous cell carcinoma (OSCC) was investigated to provide a reference for clinical prevention of OSCC cell resistance. The clinical tissues of 34 patients with OSCC-resistant cancer and 28 patients with cisplatin-sensitive OSCC in Nanjing General Hospital were collected. The expression levels of miR-27b and Frizzled-7 (FZD7) in cancer tissues were detected by reverse transcription-quantitative polymerase chain reation (RT-qPCR). After a certain gradient of cisplatin was used to induce stable acquired resistance of OSCC cell line Tca8113/CDDP, a dose of miR-27b was added to construct a cell line overexpressing miR-27b in the drug-resistant cell line. The effect of cisplatin on the proliferation of drug-resistant OSCC cells was detected by colony formation assay. In addition, the scratch test and Transwell formation assay was performed to examine the effect of cisplatin drug stimulation on proliferation and migration of Tca8113/CDDP. Flow cytometry and Hoechst 33258 staining were used to detect the effect of miR-27b on apoptosis of OSCC-resistant cells after cisplatin chemotherapy. The expression level of miR-27b in cancer tissues of patients with drug-resistant OSCC was significantly lower than that of patients with OSCC cisplatin sensitivity (P<0.05). After high expression of miR-27b, the number of clones of drug-resistant OSCC cells after adding cisplatin drugs can be significantly inhibited. The proliferation and migration ability of drug-resistant OSCC was significantly decreased after the addition of cisplatin in miR-27b overexpression (P<0.05). miR-27 mimic enhanced the pro-apoptotic ability of cisplatin drugs (P<0.05). The expression of FZD7 in cisplation-resistant patients was significantly higher (P<0.05). miR-27b significantly inhibited the expression of FZD7 and ß-catenin proteins. miR-27b can inhibit the resistance of OSCC to cisplatin drugs, increase apoptosis of cancer cells, and inhibit the proliferation of cancer cells. The mechanism may be related to the inhibition of FZD7/ß-catenin signaling pathway activation in drug-resistant cell lines by miR-27b.

6.
EBioMedicine ; 44: 665-674, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30922964

RESUMO

BACKGROUND: Increasing evidence indicates that the gut microbiota contributes to the occurrence and development of metabolic diseases. However, little is known about the effects of commonly used antidiabetic agents on the gut microbiota. In this study, we investigated the roles of dipeptidyl peptidase-4 inhibitors (DPP-4i) and α-glucosidase inhibitor in modulating the gut microbiota. METHODS: 16S-rDNA sequencing was performed to analyse the effects of DPP-4i and acarbose on the gut microbiota in mice fed a high-fat diet (HFD). Fecal microbiota transplantation (FMT) from type 2 diabetes patients to germ-free mice was performed to investigate the contribution of the altered microbiome to antidiabetic effects of the drugs. Fecal metabolomics was also analysed by untargeted and targeted GC-MS systems. FINDINGS: Although DPP-4i and α-glucosidase inhibitor both altered the gut microbial composition, only the microbiome modulation of DPP-4i contributed to its hypoglycemic effect. Specifically, the changes of 68.6% genera induced by HFD were rescued by DPP-4i. FMT showed that the DPP-4i-altered microbiome improved glucose tolerance in colonized mice, while acarbose did not. Moreover, DPP-4i increased the abundance of Bacteroidetes, and also promoted a functional shift in the gut microbiome, especially increasing the production of succinate. INTERPRETATION: Our findings demonstrate an important effect of DPP-4i on the gut microbiota, revealing a new hypoglycemic mechanism and an additional benefit of it. Furthermore, modulating the microbial composition, and the functional shift arising from changes in the microbiome, might be a potential strategy for improving glucose homeostasis. FUND: This work was supported by grants from the National Natural Science Foundation of China (No. 81700757, No. 81471039, No. 81700714 and No. 81770434), the National Key R&D Program of China (No. 2017YFC1309602, No. 2016YFC1101100, No. 2017YFD0500503 and No. 2017YFD0501001), and the Natural Science Foundation of Chongqing (No. cstc2014jcyjjq10006, No. cstc2016jcyjA0093 and No. cstc2016jcyjA0518).


Assuntos
Inibidores da Dipeptidil Peptidase IV/farmacologia , Microbioma Gastrointestinal/efeitos dos fármacos , Glucose/metabolismo , Homeostase/efeitos dos fármacos , Animais , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Glicemia , Diabetes Mellitus Tipo 2/metabolismo , Dieta Hiperlipídica , Modelos Animais de Doenças , Teste de Tolerância a Glucose , Humanos , Insulina/metabolismo , Masculino , Metagenoma , Metagenômica/métodos , Camundongos
7.
J Am Chem Soc ; 141(11): 4505-4509, 2019 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-30832476

RESUMO

Developing a facile route to access active and well-defined single atom sites catalysts has been a major area of focus for single atoms catalysts (SACs). Herein, we demonstrate a simple approach to generate atomically dispersed platinum via a thermal emitting method using bulk Pt metal as a precursor, significantly simplifying synthesis routes and minimizing synthesis costs. The ammonia produced by pyrolysis of Dicyandiamide can coordinate with platinum atoms by strong coordination effect. Then, the volatile Pt(NH3) x can be anchored onto the surface of defective graphene. The as-prepared Pt SAs/DG exhibits high activity for the electrochemical hydrogen evolution reaction and selective oxidation of various organosilanes. This viable thermal emitting strategy can also be applied to other single metal atoms, for example, gold and palladium. Our findings provide an enabling and versatile platform for facile accessing SACs toward many industrial important reactions.

8.
Arch Oral Biol ; 83: 92-96, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28735227

RESUMO

This study intended to investigate the role of microRNA-27b (miR-27b) in proliferation of oral squamous cell carcinoma (OSCC) cells and to explore the potential molecular mechanism. Cell proliferation was detected by MTT assay. The expression levels of miR-27b, Frizzled7 (FZD7), cyclin D1 and c-myc were detected by quantitative real time polymerase chain reaction (qRT-PCR). The protein expression level of FZD7 was detected by western blot analysis. The relationship between miR-27b and FZD7, and the activity of Wnt signaling pathway were determined using luciferase reporter assay. The miR-27b expression in OSCC cell lines was significantly decreased compared with control. Overexpression of miR-27b remarkably inhibited OSCC cell proliferation. Additionally, miR-27b could target and inhibit FZD7 expression and decrease the activity of Wnt signaling pathway.miR-27b could inhibit OSCC cell proliferation through inhibiting FZD7 and FZD7-mediated Wnt signaling pathway.


Assuntos
Carcinoma de Células Escamosas/patologia , MicroRNAs/metabolismo , Neoplasias Bucais/patologia , Via de Sinalização Wnt/fisiologia , Western Blotting , Linhagem Celular Tumoral , Proliferação de Células , Ciclina D1/metabolismo , Regulação para Baixo , Receptores Frizzled/metabolismo , Humanos , Proteínas Proto-Oncogênicas c-myb/metabolismo , Reação em Cadeia da Polimerase em Tempo Real
9.
Arch Med Sci ; 12(4): 881-8, 2016 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-27478471

RESUMO

INTRODUCTION: Salivary adenoid cystic carcinoma (SACC) is a frequent type of salivary gland cancer which is characterized by slow growth but high incidence of distant metastasis. We aimed to identify therapeutic targets which are associated with metastasis of SACC. MATERIAL AND METHODS: Total RNA was isolated from a low metastatic SACC cell line (ACC-2) and a highly metastatic SACC cell line (ACC-M), which was screened from ACC-2 by combination of in vivo selection and cloning in vitro. Then the total RNA was subjected to microarray analysis. Differentially expressed genes (DEGs) were screened from ACC-M compared with ACC-2, followed by Gene Ontology function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Function annotation for DEGs also was performed. A protein-protein interaction network (PPI) was constructed for DEGs. RESULTS: A total of 1128 DEGs were identified from ACC-M cells compared with ACC-2 cells. Both up- and down-regulated DEGs were enriched in different functions in biological process (BP), cellular component (CC) and molecular function (MF). Additionally, down-regulated DEGs were mainly enriched in "Apoptosis" and "Cytokine-cytokine receptor interaction" pathways which involved IFN-α1, NTRK1 and TGF-ß1. In the PPI network, PIK3CA, PTPN11 and PIK3R1 had a number of nodes greater than 10. CONCLUSIONS: Transforming growth factor ß1 might play a pivotal role during lung metastasis of SACC and be selected as a candidate target for treatment of metastatic SACC. IFNA1, NTRK1 and PIK3CA were also associated with tumor metastasis.

10.
Shanghai Kou Qiang Yi Xue ; 25(6): 688-693, 2016 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-28275791

RESUMO

PURPOSE: To investigate the effect of growth factor receptor-bound 7 (Grb7) on oral squamous cell carcinoma growth and tumor xenografts. METHODS: Cal27 and hNOK cells were cultivated, real-time PCR and Western blotting were used to detect the expression of Grb7 in hNOK and Cal27. Cal27 was transfected with Grb7 siRNA for 48 h, cell proliferation was assayed using MTT. Flow cytometry was used to determine the cell cycle and apoptosis. Western blot was used to evaluate the expression of caspase3, Bax, bcl-2, Cyclin D1, Rb, E2F1, ERK and FOXM1. Grb7 siRNA and negative control were designed and injected subcutaneously into the mice, tumor volume and weight were measured. Statistical analysis was performed using SPSS 11.0 software package. RESULTS: Grb7 was highly expressed in Cal27 compared with hNOK. Depletion of Grb7 significantly inhibited cell proliferation, blocked G1/S phase transition, promoted cell apoptosis. Knockdown of Grb7 suppressed the expression of Cyclin D1 and Rb, upregulated E2F1 expression. Moreover, c-caspase 3 and Bax was also reduced after inhibition of Grb7. ERK/FOXM1 signaling pathway was also inhibited by Grb7. In addition, the volume and weight of tumor xenografts were reduced by siGrb7. CONCLUSIONS: Depletion of Grb7 inhibits cell proliferation, promotes apoptosis and reduces tumor xenografts through ERK/FOXM1 pathway.


Assuntos
Apoptose , Carcinoma de Células Escamosas/metabolismo , Linhagem Celular Tumoral , Proliferação de Células/fisiologia , Proteína Adaptadora GRB7/metabolismo , Neoplasias Bucais/metabolismo , Animais , Proteínas Reguladoras de Apoptose , Caspase 3 , Ciclo Celular , Ciclina D1 , Proteína Adaptadora GRB7/genética , Técnicas de Silenciamento de Genes , Humanos , Sistema de Sinalização das MAP Quinases , Camundongos , RNA Interferente Pequeno , Reação em Cadeia da Polimerase em Tempo Real , Transdução de Sinais
11.
J Plast Reconstr Aesthet Surg ; 66(9): 1238-42, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23668950

RESUMO

BACKGROUND: The purpose of this study is to review our experience with the use of the local mandible myofascial (LMM) flap for anterior floor of mouth (AFOM) reconstruction following cancer ablation to assess its reliability, associated complications and functional results. METHODS: This is a retrospective analysis of 13 LMM flaps (nine patients) performed for AFOM reconstruction from March 2010 to June 2012. All patients underwent surgical resection and immediate reconstruction with LMM flaps. They were followed up for 3-30 months to evaluate the survival rate of the flaps, mobility of tongue and aesthetic outcome of the lower lip and mental region. RESULTS: All the flaps were successfully transferred. No obvious complications were found in either the AFOM or the donor region. The majority of patients resumed to a regular diet (89%, 8/9) and speech was considered as functional and/or understandable by the surgeon in all of the patients. Dental restoration was successful for 89% (8/9) of the patients. The shape and motion of the lower lip and the mental region of all patients were acceptable. CONCLUSIONS: The LMM flap is well suited for AFOM reconstruction because it is reliable, has few significant complications and allows preservation of oral function.


Assuntos
Carcinoma de Células Escamosas/cirurgia , Soalho Bucal/cirurgia , Neoplasias Bucais/cirurgia , Procedimentos Cirúrgicos Reconstrutivos/métodos , Retalhos Cirúrgicos/irrigação sanguínea , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma de Células Escamosas/patologia , China , Estudos de Coortes , Feminino , Seguimentos , Humanos , Masculino , Mandíbula/cirurgia , Pessoa de Meia-Idade , Soalho Bucal/patologia , Neoplasias Bucais/patologia , Esvaziamento Cervical/métodos , Recidiva Local de Neoplasia/patologia , Recidiva Local de Neoplasia/cirurgia , Projetos Piloto , Complicações Pós-Operatórias/fisiopatologia , Complicações Pós-Operatórias/cirurgia , Medição de Risco , Resultado do Tratamento , Cicatrização/fisiologia
12.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 47(9): 542-3, 2012 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-23141728

RESUMO

OBJECTIVE: To investigate the method and efficacy of reconstruction of anterior mouth floor defects with depressor labii inferioris flap. METHODS: Between March 2010 and August 2011, 4 patients with cancer in the anterior part of oral floor underwent tumour resection and simultaneous reconstruction with depressor labii inferioris flap. The patients were followed up for 2 - 18 months. The survival of the flaps, mobility of tongue and esthetic outcome of lip and mental region were evaluated. RESULTS: All the flaps survived completely, tongues moved well, and functional and esthetic results were good. CONCLUSIONS: The depressor labii inferioris flaps are suitable for repairing defects of anterior oral floor.


Assuntos
Músculos Faciais/cirurgia , Soalho Bucal/cirurgia , Retalhos Cirúrgicos , Idoso , Carcinoma de Células Escamosas/cirurgia , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Soalho Bucal/patologia , Neoplasias Bucais/cirurgia , Procedimentos Cirúrgicos Reconstrutivos , Cicatrização
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA