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1.
Exp Ther Med ; 18(1): 678-684, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31281447

RESUMO

The effect of histone deacetylase (HDAC)2/Inositol polyphosphate-5-phosphatase F (Inpp5f) on neuropathic pain and cognitive dysfunction through regulating PI3K/Akt/GSK-3ß signal pathway in rats with neuropathic pain was investigated. A total of 80 SPF mature male SD rats were averagely randomized into the sham operation group, the model group, the HDAC2 intervention group (group A) and the Inpp5f intervention group (group B). The rat models of neuropathic pain were established in the model group, and groups A and B. At the 15th day after modeling, rats in group A were transfected with the interference vector of HDAC2, and rats in group B were transfected with the overexpression vector of Inpp5f. Rats in the four groups were observed before modeling, after modeling/before intervention and 3 days after intervention in terms of paw thermal withdrawal latency (PWL), paw withdrawal mechanical threshold (PWT) and changes in cognitive function (Morris water maze and passive avoidance task). Then the rats were sacrificed. RT-qPCR and western blot analysis were used to detect the levels of HDAC2 mRNA, Inpp5f mRNA, phosphorylated PI3K (p-PI3K), phosphorylated AKT (p-AKT), phosphorylated GSK-3ß (p-GSK-3ß) in rat brain tissue. Correlation of HDAC2 mRNA with Inpp5f mRNA expression levels was detected by Pearsons correlation analysis. Compared with the sham operation group, PWL was significantly lower while PWT was higher in the other 3 groups (P<0.05). Three days after intervention, PWL was significantly higher while PWT was significantly lower (P<0.05). Inhibiting the expression of HDAC2 or promoting the expression of Inpp5f can effectively improve cognitive function in rats (P<0.05). After intervention, compared with the sham operation group, rats in the other 3 groups had higher HDAC2 mRNA level and lower Inpp5f mRNA level (P<0.05). In conclusion, neuropathic pain can cause an increase in HDAC2 expression level and a decrease in Inpp5f expression level, and activate the PI3K/Akt/GSK-3ß signal pathway. Inhibition of HDAC2 expression can inhibit the activation of PI3K/Akt/GSK-3ß signal pathway through increasing Inpp5f expression, thus improving the condition and cognitive disorder of rats with neuropathic pain.

2.
Biomol Ther (Seoul) ; 27(4): 414-422, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31189298

RESUMO

There is accumulating evidence that microRNAs are emerging as pivotal regulators in the development and progression of neuropathic pain. MicroRNA-15a/16 (miR-15a/16) have been reported to play an important role in various diseases and inflammation response processes. However, whether miR-15a/16 participates in the regulation of neuroinflammation and neuropathic pain development remains unknown. In this study, we established a mouse model of neuropathic pain by chronic constriction injury (CCI) of the sciatic nerves. Our results showed that both miR-15a and miR-16 expression was significantly upregulated in the spinal cord of CCI rats. Downregulation of the expression of miR-15a and miR-16 by intrathecal injection of a specific inhibitor significantly attenuated the mechanical allodynia and thermal hyperalgesia of CCI rats. Furthermore, inhibition of miR-15a and miR-16 downregulated the expression of interleukin-1ß and tumor-necrosis factor-αin the spinal cord of CCI rats. Bioinformatic analysis predicted that G protein-coupled receptor kinase 2 (GRK2), an important regulator in neuropathic pain and inflammation, was a potential target gene of miR-15a and miR-16. Inhibition of miR-15a and miR-16 markedly increased the expression of GRK2 while downregulating the activation of p38 mitogen-activated protein kinase and NF-κB in CCI rats. Notably, the silencing of GRK2 significantly reversed the inhibitory effects of miR-15a/16 inhibition in neuropathic pain. In conclusion, our results suggest that inhibition of miR-15a/16 expression alleviates neuropathic pain development by targeting GRK2. These findings provide novel insights into the molecular pathogenesis of neuropathic pain and suggest potential therapeutic targets for preventing neuropathic pain development.

3.
BMC Anesthesiol ; 19(1): 71, 2019 05 10.
Artigo em Inglês | MEDLINE | ID: mdl-31077157

RESUMO

BACKGROUND: This report describes one case of anesthetic management about surgical resection of a malignant phaeochromocytoma with tumor extension into vena cava and right atrium in a patient. Report for anesthetic management is limited in these patients under surgical resection until now. CASE PRESENTATION: In September 2015, a 24-year-old male presented to the department of cardiology with right flank pain and hypertensive urgency in our hospital. Contrast-enhanced CT abdomen and MRI abdomen revealed a mass phaeochromocytoma in right adrenal, which invaded the right inferior vena cava(IVC)wall along with IVC thrombus. Echocardiography shown no abnormal detection. Finally, this patient gave up the surgical resection of phaeochromocytoma and chose the expectant treatment. In April 2018, this patient once again presented to the emergence department in our hospital, he had experienced persistent cough and intermittent wheezing for 5 h. Contrast-enhanced CT and echocardiography shown existing IVC thrombus had extended into the right atrium. After the careful preoperative preparation, adrenalectomy with complete thrombus excision by inferior vena cava exploration and right atriotomy were performed successfully by a multidisciplinary team. After one month post-operation care, this patient healthily left our hospital. CONCLUSION: To the best of our knowledge, the occurrence of pheochromocytoma with IVC and right atrium thrombosis has not been reported in mainland China so far. This clinical case may supply a rare reference experience for surgical treatment and anesthetic management in the group of phaeochromocytoma patient with distance vascular extension.

4.
Exp Ther Med ; 17(2): 1300-1305, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30680006

RESUMO

Hdac2/Inpp5f on the therapeutic effect of pregabalin on neuropathic pain in chronic constriction injury (CCI) rats was investigated. A total of 90 healthy SD rats were selected and divided into the treatment group, the model control group and the normal control group with 30 rats in each group. Rats in the treatment and model control groups were used to construct CCI model. The control group was treated with sham surgery. The model control group was not treated after surgery. The treatment group was treated with pregabalin on the 8th day after operation. Behavioral experiments were performed to measure mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL). The expression of inflammatory cytokines TNFα and IL-1ß was detected by ELISA kit and expression of Hdac2 and Inpp5f mRNA was detected by RT-qPCR. Before treatment, compared with the normal control group, MWT and TWL values of the treatment and model control groups were significantly decreased (P<0.05). At different time-points after treatment, MWT and TWL were higher in the treatment group than in the model control group and were lower than in the normal control group. At 21 days after operation, the levels of TNFα and IL-1ß in the model control and treatment groups were higher than those in the normal control group (P<0.05). The expression level of Hdac2 was higher in the treatment group than in the normal control group (P<0.05). The expression level of Inpp5f mRNA in the treatment and normal control groups was significantly higher than that in the model control group (P<0.05), but the expression level of Inpp5f mRNA in the treatment group was lower than that in the normal control group (P<0.05). Therefore, pregabalin can effectively relieve neuropathic pain in CCI rats, and its efficacy is related to Hdac2 and Inpp5f. Our study provides reference for clinical treatment of neuropathic pain induced by CCI.

5.
Exp Ther Med ; 16(3): 1963-1967, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30186425

RESUMO

Osteoporosis (OP) is a common serious skeletal disorder marked by increased risk of bone fracture due to fragility. OP has been taken to be a disease linked with abnormal calcium metabolism that alone is obviously insufficient to explain the development of OP. Iron overload has been associated with the development of OP and increasing studies have suggested the association. However, direct evidence for this has not been clinically established. To this end, using the Roche biochemical autoanalyzer, we detected the concentration of iron, soluble transferrin receptor 2 (TFR2), and hepcidin, a key peptide regulating iron homeostasis, in the sera from patients with OP. It was shown that the iron and TFR2 concentration was markedly higher than that of healthy control; whereas the concentration of hepcidin was markedly lower than that in control. In addition, to pilot explore the underlying mechanism by which hepcidin was downregulated, we present that hepcidin can directly interact with TFR2 using immunoprecipitation. The present study first established the direct biochemical evidence for the involvement of hepcidin in the pathogenesis of OP, indicating that the upregulation of hepcidin could be used as a novel alternative therapeutic strategy in the management of OP.

6.
Nanoscale Res Lett ; 11(1): 126, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26951126

RESUMO

In this report, a novel photocatalyst based on Bi2WO6/Ag2S heterostructures was prepared by a 3-mercaptopropionic acid (MPA)-assisted route at room temperature. Compared to bare Bi2WO6 and Ag2S nanoparticles, the as-formed Bi2WO6/Ag2S heterostructures exhibit enhanced photocatalytic activity for the degradation of rhodamine B (Rh B) under visible-light irradiation. This kind of enhancement in the photocatalytic activity is considered to be the synergistic effects of both the effective electron-hole separation and expansion of the light-absorption range. The pH of the solution is of vital importance to the photocatalytic activity of the as-formed Bi2WO6/Ag2S heterostructures. Under low pH value, the photosensitization process is suppressed, while under higher pH value, the photosensitization process is favored. The mechanism of the photocatalytic process was proposed by the active-species-trapping experiments, indicating that the photogenerated holes (h(+)) play a crucial role in the degradation of Rh B under visible light. The enhanced photocatalytic performance of this heterostructure makes it a promising material for the treatment of dye-containing wastewater.

7.
J Colloid Interface Sci ; 466: 388-99, 2016 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-26752434

RESUMO

Highly-efficient photocatalyst based on Bi2WO6/SnS heterostructure was prepared via a surface functionalization method using 3-mercaptopropionic (MPA) as the surface functionalizing agent. Compared to bare Bi2WO6 and SnS nanoparticles, the as-formed Bi2WO6/SnS heterostructure exhibits enhanced photocatalytic activity for the degradation of Rhodamine B (Rh B). Photoluminescence and photocurrent measurements demonstrate that the enhanced photocatalytic activity during the photocatalytic process is closely related to the enhanced electron-hole separation efficiency. The photocatalytic activity of the as-formed Bi2WO6/SnS heterostructure can be perfectly remained even after being used for five times, showing excellent durability during the photocatalytic process. The influence of pH and inorganic ions are systematically investigated. And the optimum pH for the photocatalytic process is determined to be 6. The addition of chloride ion will exert negative effect on the photodegradation process of Rh B. The mechanism of photodegradation process was investigated by exploring the quenching effects of different scavengers and the results suggest that the reactive holes play the major role in the photodegradation process of Rh B.

8.
J Biomed Opt ; 20(5): 051011, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25428579

RESUMO

Hair follicle offers an excellent model for systems biology and regenerative medicine. So far, the stages of hair follicle growth have been evaluated by histological examination. In this work, a noninvasive spectroscopy was proposed by measuring the diffuse reflectance of mouse skin and analyzing the melanin value. Results show that the skin diffuse reflectance was relatively high when hair follicles were at the telogen stage and at the beginning of the anagen stage, and decreased with the progression of the anagen stage. When the hair follicle entered into the catagen stage, the diffuse reflectance gradually increased. The changes in the melanin content of skin had contrary dynamics. Substages of the hair follicle cycle could be distinguished by comparing the changes in melanin value with the histological examination. This study provided a new method for noninvasive evaluation of the hair follicle stage, and should be valuable for basic and therapeutic investigations on hair regeneration.


Assuntos
Folículo Piloso/fisiologia , Cabelo/crescimento & desenvolvimento , Espectrofotometria/métodos , Animais , Feminino , Folículo Piloso/crescimento & desenvolvimento , Melaninas/química , Camundongos , Camundongos Endogâmicos C57BL , Óptica e Fotônica , Regeneração , Pele/metabolismo , Pigmentação da Pele
9.
J Colloid Interface Sci ; 432: 236-45, 2014 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-25086718

RESUMO

Controlled synthesis of well-shaped nanocrystals is of significant importance to understand the surface-related properties. Herein, hierarchical Bi2WO6 particles with different morphologies, such as flower-like and pancake-like morphologies were selectively prepared using a simple fluoride ion-assisted hydrothermal process. Morphological modulation of the samples could be easily realized by controlling the initial amount of NH4F. The effect of NH4F as well as the formation mechanism of these Bi2WO6 hierarchical structures were systematically investigated. The morphological control of the final products are proved to be a kinetic control of the reaction, which is closely related to the concentration of fluoride ion in the solution. The as-obtained hierarchical Bi2WO6 particles exhibit different visible-light-driven photo-catalytic activities for the degradation of Rhodamine-B (RhB). The differences in photo-catalytic activities among the as-obtained samples are associated the surface adsorption properties, which result from the synthetic conditions.

10.
J Biomed Opt ; 18(6): 61228, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23429836

RESUMO

Lasers have shown great advantages in enhancing transdermal drug delivery. However, the physical or physiological mechanisms are not clear, which limits the application in clinical medicine. Here, 1064 nm-Nd:YAG lasers with long-pulsed (LP, 15 J/cm²) and Q-switched (QS, 0.5 J/cm²) output modes inducing short- and long-term effects on the stratum corneum (SC) of skin are investigated. Infrared thermography is applied to monitor the dynamical temperature distribution of the skin surface, while histopathological analysis and two-photon fluorescence microscopy are employed to examine changes in the microstructure of skin and molecular constitution of SC, respectively. Results have shown that the LP laser irradiation increases skin temperature evidently and loosens keratin, making corneocytes fragile or exfoliative, whereas the QS laser irradiation disrupts the keratin or corneocytes completely, perforating some micropores on the SC. It can be concluded that the mechanisms of enhancing transdermal delivery caused by lasers depends on the output modes. The LP laser irradiation produces thermal effects on skin, which loosens the SC, while the QS laser induces mechanical effects on skin, which punches micropores on the SC. Moreover, the laser-induced enhancing effects on transdermal glycerol delivery can last for one week to wait for the recovery of SC.


Assuntos
Administração Cutânea , Sistemas de Liberação de Medicamentos/métodos , Lasers de Estado Sólido/uso terapêutico , Animais , Queratinas/metabolismo , Masculino , Microscopia de Fluorescência por Excitação Multifotônica , Fenômenos Ópticos , Ratos , Ratos Wistar , Pele/efeitos dos fármacos , Pele/metabolismo , Pele/efeitos da radiação , Temperatura Cutânea/efeitos da radiação , Termografia
11.
Acta Crystallogr Sect E Struct Rep Online ; 67(Pt 4): o1023, 2011 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-21754039

RESUMO

In the title compound, C(10)H(11)NO(2)S, the acetyl-acetone group crystallizes in the keto form with all the non-hydrogen atoms in the acetyl-acetone group approximately co-planar with a maximum atomic deviation 0.055 (2) Å; the dihedral angle between the acetyl-acetone group and the pyridine ring is 85.90 (6)°. An intra-molecular O-H⋯O hydrogen bond involving the acetyl-acetone group forms a six-membered ring.

12.
Zhonghua Wai Ke Za Zhi ; 48(6): 432-4, 2010 Mar 15.
Artigo em Chinês | MEDLINE | ID: mdl-20627006

RESUMO

OBJECTIVE: To observe the effects of "wake-up correction" technique for preventing iatrogenic spinal cord injury in scoliosis surgery. METHODS: Twenty-one patients who had scoliosis with Cobb's angle 92 degrees - 145 degrees received operation of pedicle screw insertion in all or important vertebral bodies, release of stiff segments, decompression and osteotomy. All the patients were trained how to wake up before anesthesia. Maintenance of anesthesia was achieved with infusion of propofol at target-controlled concentration 3-4 mg/L and remifentanil at 0.15 microg/(kg.min). Fresh gas 2 L/min of N(2)O:O(2) 1:1 was inhaled during mechanical ventilation. Wake-up methods:the muscle relaxant was stopped injection 30 min before wake-up, decreasing propofol's target-controlled concentration to 1-2 mg/L and remifentanil to 0.05 - 0.10 microg/(kg x min). Once the spontaneous respiration returned, woke up the patients and asked them move both toes following our orders (the first wake-up). Then patients inhaled 6% sevoflurane in fresh gas 6 L/min (N(2)O:O(2) 1:1). When the end-tidal anesthetic gas concentration was arrived 1.3 - 1.5 MAC, all of the anesthetics were stopped. The correction operation was completed and the patient was woke up again (the second wake-up). Recorded data included time used to wake up, directive action returning time, whether the patient had memory of wake-up during operation when following up. RESULTS: All patients woke up with satisfaction. The time taken the first wake-up was (10.3 + or - 4.5) min, and for the second was (4.3 + or - 2.3) min. There were two patients who had slightly agitation during correction. There was no one who had neurological injury. There was no memory of wake-up and no pain in all patients during operation. Cobb' angle was corrected to 22 degrees - 38 degrees (average 29 degrees ), and the correction rate was 74%. CONCLUSION: The "wake-up correction" is effective and satisfactory by detecting the cord function in time.


Assuntos
Escoliose/cirurgia , Traumatismos da Medula Espinal/prevenção & controle , Fusão Vertebral/enfermagem , Adolescente , Adulto , Feminino , Humanos , Doença Iatrogênica/prevenção & controle , Complicações Intraoperatórias/prevenção & controle , Masculino , Traumatismos da Medula Espinal/etiologia , Fusão Vertebral/métodos , Adulto Jovem
13.
Lasers Surg Med ; 42(2): 132-40, 2010 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-20166162

RESUMO

BACKGROUND AND OBJECTIVES: Tissue optical clearing technique based on immersion of tissues into optical clearing agents (OCAs) can reduce the scattering and enhance the penetration of light in tissue. However, the barrier function of epidermis limits the penetration of OCAs, and hence is responsible for the poor optical clearing efficacy of skin by topical action. In this study, a variety of light irradiation was applied to increase permeability of agents in skin and improve the optical clearing efficacy. STUDY DESIGN/MATERIALS AND METHODS: Different light sources with different dose, i.e, CO(2) laser, Nd:YAG laser (532 and 1,064 nm) with different pulse modes and Intense Pulsed Light (IPL) (400-700 and 560-950 nm) were used to irradiate rat skin in vivo, and then glycerol was applied onto the irradiated zone. VIS-NIR spectrometer was utilized to monitor the changes of reflectance. In vitro skin samples were also irradiated by Q-switched Nd:YAG laser (1,064 nm) and then treated by glycerol for 10-60 minutes. Based on the measurement of the reflectance and transmittance of the samples, the optical properties of skin and penetration depth of light were calculated. RESULTS: Results show that photo-irradiation with appropriate dose combining with the following glycerol treatment is able to reduce in vivo skin reflectance. Compared with the control group, the maximal changes in reflectance are ninefold at 575 nm and eightfold at 615 nm, respectively, which were caused by Q-switched 1,064-nm Nd:YAG laser irradiation and following glycerol treatment. The results for in vitro skin demonstrate that the joint action can significantly increase the optical penetration depth in samples. CONCLUSIONS: The combination of Q-switched Nd:YAG (1,064 nm) laser and glycerol could enhance optical skin clearing efficacy significantly. This study provides a non-invasive way to improve the optical clearing of skin, which will benefit the skin optical therapy.


Assuntos
Glicerol/administração & dosagem , Glicerol/farmacocinética , Terapia com Luz de Baixa Intensidade/métodos , Pele/metabolismo , Administração Cutânea , Animais , Lasers , Lasers de Gás/uso terapêutico , Lasers de Estado Sólido/uso terapêutico , Masculino , Modelos Animais , Permeabilidade , Fotoquimioterapia/métodos , Probabilidade , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Espalhamento de Radiação , Pele/efeitos dos fármacos , Pele/efeitos da radiação , Absorção Cutânea/efeitos dos fármacos , Absorção Cutânea/efeitos da radiação , Espectrofotometria , Tomografia de Coerência Óptica , Resultado do Tratamento
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