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1.
Sci Total Environ ; 855: 158924, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36152845

RESUMO

Mono(2-ethylhexyl) phthalate (MEHP) is a metabolite of DEHP which is one of phthalic acid esters (PAEs) widely used in daily necessities. Moreover, MEHP has been proven to have stronger biological toxicity comparing to DEHP. In particular, several recent population-based studies have reported that intrauterine exposure to MEHP results in adverse pregnancy outcomes. To explore the mechanisms and metabolic biomarkers of MEHP exposure, we examined the metabolic status of HTR-8/Svneo cell lines exposed to different doses of MEHP (0, 1.25, 5.0, 20 µM). Global and dose-response metabolomics tools were used to identify metabolic perturbations and sensitive markers associated with MEHP. Only 22 metabolic features (accounted for <1 %) were significantly changed when exposed to 1.25 µM. However, when the exposure dose was increased to 5 or 20 µM, the number of significantly changed metabolic features exceeded 300 (approximately 10 %). In particular, amino acid metabolism, pyrimidine metabolism and glutathione metabolism were widely affected according to the enrich analysis of those significant altered metabolites, which has and have previously been reported to be closely related to fetal development. Moreover, 5'-UMP and N-acetylputrescine with the lowest effective concentrations (EC-10 = 0.1 µM and EC+10 = 0.11 µM, respectively) were identified as sensitive endogenous biomarkers of MEHP exposure.


Assuntos
Dietilexilftalato , Ácidos Ftálicos , Gravidez , Feminino , Humanos , Dietilexilftalato/toxicidade , Dietilexilftalato/metabolismo , Trofoblastos/química , Trofoblastos/metabolismo , Ácidos Ftálicos/toxicidade , Ácidos Ftálicos/metabolismo , Biomarcadores/análise
2.
Food Chem ; 402: 134227, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36155290

RESUMO

In this study, we compared the incidence of postharvest disease and the storage potential of blueberry fruit treated with thymol (inoculated with Aspergillus niger) with those in the control fruit during storage for 42 d at 2 °C. Treatment with 10-30 mg/L thymol was found to be more effective than treatment with higher thymol concentrations of 40-50 mg/L in terms of controlling Aspergillus niger-induced decay. In the thymol-treated blueberry fruit peel, the activities of the disease resistance-related enzymes were significantly enhanced. Furthermore, the 20 mg/L thymol-treated blueberry fruit retained the highest firmness, total soluble solids content, and acceptability score, and it also maintained an unimpaired cell wall structure of pericarp quality. Thus, low-dose thymol-treatment could be a suitable biocontrol agent for controlling postharvest disease and prolonging the storage life of blueberry fruit.


Assuntos
Mirtilos Azuis (Planta) , Mirtilos Azuis (Planta)/química , Frutas/química , Timol/farmacologia , Antioxidantes/análise , Parede Celular
3.
Blood Cells Mol Dis ; 98: 102708, 2023 01.
Artigo em Inglês | MEDLINE | ID: mdl-36334505

RESUMO

Severe aplastic anemia (SAA) is a bone marrow failure disorder caused by autoimmune dysfunction. The presentation by dendritic cells (DCs) is the key step in initiating the immune response against unknown antigens in SAA patients. In the previous phase, we found that compared to healthy controls, patients with SAA had an increased proportion of circulating myeloid/conventional dendritic cells (mDCs/cDCs) with enhanced phagocytosis, more secretion of Th1-type cytokines (IL-2, TNF-α, IFN-γ) in the bone marrow, and a reduced proportion of Treg cells. In this study, we found that cDCs sorted from SAA patients had higher expression level of HLA-DQ, co-stimulatory molecules CD86, PTK and ERK1/2 than the remission SAA patients and healthy controls. Moreover, downregulation of HLA-DQ protein levels on cDCs derived from SAA patients resulted in reduced phagocytosis rate and CD86 expression of cDCs. When the cDCs above were co-cultured with CD4+ cells from the same patients, reduced secretion of Th1 type of lymphocyte cytokines was observed. Analysis of clinically relevant data suggests that HLA-DQ expression levels were closely related to disease severity and immune status of patients. These findings show that the role of HLA-DQ in the immunopathogenesis of SAA is potentially important and worth further study.


Assuntos
Anemia Aplástica , Humanos , Medula Óssea/patologia , Fator de Necrose Tumoral alfa , Antígenos HLA-DQ/metabolismo
4.
Appl Psychol Meas ; 47(1): 34-47, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36425288

RESUMO

In equating practice, the existence of outliers in the anchor items may deteriorate the equating accuracy and threaten the validity of test scores. Therefore, stability of the anchor item performance should be evaluated before conducting equating. This study used simulation to investigate the performance of the t-test method in detecting outliers and compared its performance with other outlier detection methods, including the logit difference method with 0.5 and 0.3 as the cutoff values and the robust z statistic with 2.7 as the cutoff value. The investigated factors included sample size, proportion of outliers, item difficulty drift direction, and group difference. Across all simulated conditions, the t-test method outperformed the other methods in terms of sensitivity of flagging true outliers, bias of the estimated translation constant, and the root mean square error of examinee ability estimates.

5.
Sci Total Environ ; 857(Pt 2): 159527, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36270365

RESUMO

Knowledge on driving forces controlling natural background levels (NBLs) of geogenic contaminants (GCs) in groundwater of coastal urbanized areas are still limited because of complex hydrogeological conditions and anthropogenic activities. This study assesses NBLs of two GCs including arsenic (As) and manganese (Mn) in four groundwater units of the Pearl River Delta (PRD) with large scale urbanization by using a preselection method composed of the chloride/bromide mass ratio versus chloride concentration and the oxidation capacity with the combination of Grubbs' test. More importantly, driving factors controlling NBLs of As/Mn in groundwater of the PRD are discussed. Results showed that groundwater As/Mn concentrations in residual datasets were independent of land-use types, while those in original datasets in different land-use types were distinct because of various human activities, indicating that the used preselection method in this study is valid for NBLs-As/Mn assessment in groundwater of the PRD. NBL-As in coastal-alluvial aquifers was >6 times that in other groundwater units. NBL-Mn in coastal-alluvial aquifers was 1.4 times that in alluvial-proluvial aquifers, and both were >4 times that in other two groundwater units. High NBLs-As/Mn in coastal-alluvial aquifers is mainly attributed to reduction of FeMn oxyhydr(oxides) induced by mineralization of organic matter in Quaternary sediments. Elevated pH also contributes higher NBL-As in coastal-alluvial aquifers. By contrast, higher NBL-Mn in alluvial-proluvial aquifers than in other two groundwater units mainly ascribes to reduction of FeMn oxyhydr(oxides) in Quaternary sediments triggered by irrigation of reducing river waters. In addition, more occurrence of As/Mn-rich sediments and the infiltration of As/Mn-rich river water are also important factors for high NBLs-As/Mn in coastal-alluvial aquifers. This study shows that revealing natural driving factors of GCs-rich groundwater in coastal urbanized areas on the basis of identification of contaminated groundwaters via the used preselection methods is acceptable.


Assuntos
Arsênio , Água Subterrânea , Poluentes Químicos da Água , Humanos , Monitoramento Ambiental , Cloretos , Poluentes Químicos da Água/análise , Arsênio/análise , Manganês , Óxidos
6.
Brain Stimul ; 2022 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-36460293

RESUMO

BACKGROUND: Treating neuropsychiatric symptoms (NPS) in Alzheimer's disease (AD) remains highly challenging. Noninvasive brain stimulation using repetitive transcranial magnetic stimulation (rTMS) or transcranial direct current stimulation (tDCS) is of considerable interest in this context. OBJECTIVE: To investigate the efficacy and safety of a novel technique involving simultaneous application of rTMS and tDCS (rTMS-tDCS) over bilateral angular gyrus (AG, P5/P6 electrode site) for AD-related NPS. METHODS: Eighty-four AD patients were randomized to receive rTMS-tDCS, single-rTMS, single-tDCS, or sham stimulation for 4 weeks, with evaluation at week-4 (W4, immediately after treatment) and week-12 (W12, follow-up period) after initial examination. Primary outcome comprising Neuropsychiatric Inventory (NPI) score and secondary outcomes comprising mini-mental state examination (MMSE), AD assessment scale-cognitive subscale (ADAS-cog), and Pittsburgh sleep quality index (PSQI) scores were collected and analyzed by a two-factor (time and treatment), mixed-design ANOVA. RESULTS: rTMS-tDCS produced greater improvement in NPI scores than single-tDCS and sham at W4 and W12 (both P < 0.017) and trended better than single-rTMS (W4: P = 0.058, W12: P = 0.034). rTMS-tDCS improved MMSE scores compared with single-tDCS at W4 (P = 0.011) and sham at W4 and W12 (both P < 0.017). rTMS-tDCS also significantly improved PSQI compared with single-rTMS and sham (both P < 0.017). Interestingly, rTMS-tDCS-induced NPI/PSQI improvement was significantly associated with MMSE/ADAS-cog improvement. tDCS- and/or rTMS-related adverse events appeared slightly and briefly. CONCLUSIONS: rTMS-tDCS application to bilateral AG can effectively improve AD-related NPS, cognitive function, and sleep quality with considerable safety.

7.
Mol Carcinog ; 2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36342357

RESUMO

Dihydroartemisinin (DHA) has recently attracted increasing attention for its low toxicity and high antitumor activity. DHA has been reported to have synergistic anticancer effects with a variety of drugs in the clinic; however, the molecular mechanism by which DHA inhibits tumorigenesis and improves oxaliplatin cytotoxicity in colon cancer cells is still not well understood. In this study, we found that DHA can inhibit cell proliferation and colony formation in a dose-dependent manner. Prohibitin 2 (PHB2) is a potential target by which DHA exerts its antitumor and cytotoxic effects. The function and molecular mechanism of PHB2 in colon cancer tumorigenesis were fully studied to determine the regulatory mechanism between DHA and PHB2. We found that PHB2, a mitochondrial inner membrane scaffold protein, has a higher expression level in colon cancer tissues than in adjacent nontumor tissues and is mainly localized in mitochondria. Overexpression of PHB2 can promote cell proliferation and colony formation in vitro and accelerate tumor growth in vivo. We also found that the expression level of PHB2 was inversely related to the cytotoxicity of DHA and oxaliplatin in colon cancer cells. The molecular mechanism of PHB2 in tumorigenesis and cancer therapy was further studied. The results showed that 20 µM DHA can downregulate PHB2 expression in a ubiquitylation-dependent manner and subsequently block PHB2-induced RCHY1 upregulation and p53 and p21 downregulation. In this process, RCHY1 is necessary for PHB2 to play a tumor-promoting role. Thus, PHB2 and RCHY1 are effective targets for colon cancer therapy, and DHA has synergistic anticancer effects with oxaliplatin via promoting PHB2 degradation in colon cancer cells.

8.
Cell Stress Chaperones ; 27(6): 703-715, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36327089

RESUMO

Reactive oxygen species (ROS) production is critical for the initiation of wound repair; however, persistently high levels of ROS can lead to lipid peroxidation in cells and thus affect wound healing. Iron is a transition metal that is an essential component of almost all living cells and organisms. When present in excess in cells and tissues, iron disrupts redox homeostasis and catalyzes the generation of ROS, leading to increased lipid peroxidation. In this study, we found that after treating interfollicular epidermal (IFE) cells with different concentrations of holotransferrin (0 µg/ml, 1 µg/ml, 10 µg/ml, 100 µg/ml, and 1 mg/ml), the intracellular iron content increased, and cell viability and function did not differ significantly among the treatment groups of cells. In addition, the level of lipid peroxidation in IFE cells did not increase following holotransferrin treatment. We speculated that there is a protective mechanism within IFE cells that reduces the occurrence of intracellular lipid peroxidation. We also found that the elevated intracellular iron content of IFE cells was accompanied by elevated ALDH3B1 expression. We investigated the effect of ALDH3B1 on the level of lipid peroxidation in IFE cells and found that elevated ALDH3B1 expression decreased the damage to IFE cells induced by lipid peroxidation. In addition, the NRF2 pathway was found to affect the expression of ALDH3B1, which in turn affected lipid peroxidation in IFE cells. These findings suggest that in IFE cells, activation of the NRF2 pathway can increase the expression of ALDH3B1 and thus reduce the production of intracellular ROS and the occurrence of intracellular lipid peroxidation. Therefore, ALDH3B1 may be a potential target for the treatment of chronic wounds.


Assuntos
Ferro , Fator 2 Relacionado a NF-E2 , Peroxidação de Lipídeos , Espécies Reativas de Oxigênio/metabolismo , Fator 2 Relacionado a NF-E2/metabolismo , Células Epidérmicas/metabolismo
9.
Artigo em Inglês | MEDLINE | ID: mdl-36378368

RESUMO

Low-carbon development in BRI countries is crucial to the global response to climate change. As an important factor in promoting economic growth, financial development has a great impact on BRI countries' carbon emissions. This paper applies the panel data of 43 BRI countries for the period 1997-2019 to investigate the influence of financial development on carbon emissions. The two-tier stochastic Frontier model is employed to decompose the restraining and rebounding effects of financial development. The results show that financial development has both restraining and rebounding effects on carbon emissions, and the rebounding effect is less than the restraining effect, resulting in a positive net effect. The average restraining effect is 0.165, and the average rebounding effect is 0.018, causing the net effect of financial development on carbon emissions to have an average value of 0.147. The restraining effect decreases year by year, implying there is a blocking point to release the restraining effect. Moreover, technological progress strengthens the restraining effect, while the increase in energy consumption weakens the restraining effect. The findings indicate that excavating the restraining effect of financial development, promoting technological progress, and reducing fossil energy consumption is crucial to promoting the low-carbon development of BRI countries.

10.
Front Plant Sci ; 13: 999757, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36388585

RESUMO

Photorespiration has emerged as a hotspot in the evolution of photosynthesis owing to the energy loss during the process. To ensure the physiological functions of photorespiration such as light protection, H2O2 signaling, and stress resistance, separate the photorespiration glycolic acid flow, and minimize photorespiration loss, a balance must be maintained during the construction of photorespiratory metabolic branch. In this study, glycolate oxidase (GLO) and catalase (CAT) were introduced into potato (Solanum tuberosum) chloroplasts through the expression of fusion protein. Through the examination of phenotypic characteristics, photosynthesis, anatomical structure, and enzyme activity, the efficiency of the photorespiration pathway was demonstrated. The results showed that certain transgenic lines plants had shorter plant height and deformed leaves and tubers in addition to the favorable photosynthetic phenotypes of thicker leaves and larger and denser mesophyll cells. By Diaminobenzidine (DAB) staining analysis of the leaves, the intermediate H2O2 could not be decomposed in time to cause biomass decline and malformation, and the excessive glycolate shunt formed by the overexpression of the fusion protein affected other important physiological activities. Hence, the appropriate and coordinated expression of glycolate oxidase and catalase is essential for the establishment of photorespiration pathways in chloroplasts.

11.
Artigo em Inglês | MEDLINE | ID: mdl-36429607

RESUMO

(1) Background: To quantitatively analyze the content structure and psychological mechanism of Chinese college students' school sports policy attitude; (2) Methods: This paper uses the data of 2112 college students obtained from a national sample survey. We also use a theoretical model of school sports policy attitude empirically tested by the LISREL structural equation model method; (3) Results: ① Students' cognition of the content, implementation, effect, satisfaction, and policy behavior of school sports policies are important factors affecting the implementation of school sports policy. ② Cognition generates emotions and students' awareness of school sports policy. The higher the recognition of content, policy implementation, and policy effect, the higher their satisfaction with the school sports policy. ③ Emotion determines behavior. The greater the students' satisfaction with the school sports policy, the more willing they are to follow the school sports policy requirements and demonstrate positive behavior in line with its provisions. ④ The measurement model of the school sports policy attitude has good reliability and validity, and the theoretical hypothesis of the structural model is supported by the data that measures and explains the college students' attitudes toward school sports policy.


Assuntos
Políticas , Estudantes , Humanos , Reprodutibilidade dos Testes , Atitude , China , Instituições Acadêmicas
12.
Int J Mol Sci ; 23(22)2022 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-36430663

RESUMO

Soybean is a pivotal staple crop worldwide, supplying the main food and feed plant proteins in some countries. In addition to interacting with mutualistic microbes, soybean also needs to protect itself against pathogens. However, to grow inside plant tissues, plant defense mechanisms ranging from passive barriers to induced defense reactions have to be overcome. Pathogenic but also symbiotic micro-organisms effectors can be delivered into the host cell by secretion systems and can interfere with the immunity system and disrupt cellular processes. This review summarizes the latest advances in our understanding of the interaction between secreted effectors and soybean feedback mechanism and uncovers the conserved and special signaling pathway induced by pathogenic soybean cyst nematode, Pseudomonas, Xanthomonas as well as by symbiotic rhizobium.


Assuntos
Fabaceae , Rhizobium , Soja , Interações Microbianas , Simbiose
13.
J Cell Commun Signal ; 2022 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-36445632

RESUMO

The crucial role of macrophages in the healing of chronic diabetic wounds is widely known, but previous in vitro classification and marker genes of macrophages may not be fully applicable to cells in the microenvironment of chronic wounds. The heterogeneity of macrophages was studied and classified at the single-cell level in a chronic wound model. We performed single-cell sequencing of CD45 + immune cells within the wound edge and obtained 17 clusters of cells, including 4 clusters of macrophages. One of these clusters is a previously undescribed population of macrophages possessing osteoclast gene expression, for which analysis of differential genes revealed possible functions. We also analysed the differences in gene expression between groups of macrophages in the control and diabetic wound groups at different sampling times. We described the differentiation profile of mononuclear macrophages, which has provided an important reference for the study of immune-related mechanisms in diabetic chronic wounds.

14.
Eur J Med Res ; 27(1): 231, 2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36333771

RESUMO

Acute lung injury (ALI), a prevalent complication of severe acute pancreatitis (SAP), is also a leading contributor to respiratory failure and even death of SAP patients. Here, we intended to investigate the function and mechanism of stellate ganglion block (SGB) in ameliorating SAP-induced ALI (SAP-ALI). We engineered an SAP-ALI model in rats and treated them with SGB. HE staining and the dry and wet method were implemented to evaluate pathological alterations in the tissues and pulmonary edema. The rats serum changes of the profiles of TNF-α, IL-6, IL-1ß, and IL-10 were examined. The profiles of miR-155-5p and SOCS5/JAK2/STAT3 were detected. Functional assays were performed for confirming the role of miR-155-5p in modulating the SOCS5/JAK2/STAT3 pathway in pulmonary epithelial cells. Our findings revealed that SGB vigorously alleviated SAP rat lung tissue damage and lung edema and lessened the generation of pro-inflammatory cytokines TNF-α, IL-6, and IL-1ß. SGB enhanced SOCS5 expression, hampered miR-155-5p, and suppressed JAK2/STAT3 pathway activation. As evidenced by mechanism studies, miR-155-5p targeted the 3'UTR of SOCS5 and repressed its expression, hence resulting in JAK2/STAT3 pathway activation. During animal trials, we discovered that SGB ameliorated SAP-ALI, boosted SOCS5 expression, and mitigated the levels of pro-inflammatory factors and miR-155-5p in the plasma. In vitro, miR-155-5p overexpression substantially facilitated pulmonary epithelial cell apoptosis, inflammation, and JAK2/STAT3 pathway activation and restrained SOCS5 expression. All in all, our work hinted that SGB could modulate the miR-155-5p/SOCS5/JAK2/STAT3 axis to alleviate SAP-ALI.


Assuntos
Lesão Pulmonar Aguda , MicroRNAs , Pancreatite , Edema Pulmonar , Ratos , Animais , Pancreatite/genética , Fator de Necrose Tumoral alfa/metabolismo , Interleucina-6/metabolismo , Doença Aguda , Gânglio Estrelado/metabolismo , Gânglio Estrelado/patologia , Lesão Pulmonar Aguda/genética , Edema Pulmonar/genética , MicroRNAs/genética , MicroRNAs/metabolismo , Janus Quinase 2/genética , Janus Quinase 2/efeitos adversos
15.
Molecules ; 27(22)2022 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-36431857

RESUMO

The present study aims to analyze the structural characterization and antioxidant activity of a novel exopolysaccharide from Rhizopus nigricans (EPS2-1). For this purpose, EPS2-1 was purified through DEAE-52, Sephadex G-100, and Sephadex G-75 chromatography. The structural characterization of EPS2-1 was analyzed using high-performance gel permeation chromatography (HPGPC), Fourier transform infrared spectroscopy (FT-IR), methylation analysis, nuclear magnetic resonance (NMR) spectra, transmission electron microscope (TEM), and atomic force microscope (AFM). The results revealed that EPS2-1 is composed of mannose (Man), galactose (Gal), glucose (Glc), arabinose (Ara), and Fucose (Fuc), and possesses a molecular weight of 32.803 kDa. The backbone of EPS2-1 comprised →2)-α-D-Manp-(1→ and →3)-ß-D-Galp-(1→, linked with the O-6 position of (→2,6)-α-D-Manp-(1→) of the main chain is branch α-D-Manp-(1→6)-α-D-Manp-(1→, linked with the O-6 positions of (→3)-ß-D-Galp-(1→) of the main chain are branches →4)-ß-D-Glcp-(1→ and →3)-ß-D-Galp-(1→, respectively. Finally, we demonstrated that EPS2-1 also shows free radical scavenging activity and iron ion reducing ability. At the same time, EPS2-1 could inhibit the proliferation of MFC cells and increase the cell viability of RAW264.7 cells. Our results suggested that EPS2-1 is a novel polysaccharide, and EPS2-1 has antioxidant activity. In addition, EPS2-1 may possess potential immunomodulatory and antitumor activities. This study promoted the application of EPS2-1 as the functional ingredients in the pharmaceutical and food industries.


Assuntos
Antioxidantes , Polissacarídeos , Humanos , Antioxidantes/farmacologia , Antioxidantes/química , Espectroscopia de Infravermelho com Transformada de Fourier , Polissacarídeos/química , Peso Molecular
16.
Medicine (Baltimore) ; 101(46): e31476, 2022 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-36401474

RESUMO

INTRODUCTION: Ophiocordyceps sinensis(O. sinensis), a genus of ascomycete fungi, has been intensively studied in various disease models, which is a rich source of various bioactive compounds and used in the treatment for end-stage renal disease patients. This systematic review highlights the therapeutic roles of O. sinensis as adjuvant treatment for dialysis patients with clinical evidence. METHODS AND ANALYSIS: The systematic review will be performed according to the Cochrane Handbook for Systematic Reviews of Interventions. The protocol is being reported in accordance with the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols Statement. An literature search strategy will be developed and adapted for 9 databases. Searches will be run from the database inception until the date of the search implementation and be updated before the review is completed. Randomized controlled trials that investigate the effects of O. sinensis for dialysis patients (peritoneal dialysis and hemodialysis) will be included. We will focus on outcomes recommended by the core outcome measures in effectiveness trials, including mortality, cardiovascular disease, infection, vascular access problems, dialysis adequacy, hyperkalaemia, life participation. Two researchers will independently screen the studies, extract data and evaluate study quality using the Risk of Bias 2 tool. Subgroup analysis will be performed according to peritoneal dialysis and hemodialysis. Sensitivity analyses will be conducted based on the Leave-1-Out Method. The Grading of Recommendations Assessment, Development, and Evaluation approach will be used to rate the quality of the evidence. Meta analysis will be performed using Review Manager 5.3 and R packages. OBJECTIVES: Studies have reported positive results of O. sinensis as adjuvant treatment for patients with dialysis. This review will synthesis current evidence on how O. sinensis can improve dialysis. Thus, it is expected that robust and conclusive evidence of the effects of O. sinensis during or after treatment can be obtained. These findings can inform future research and the selection of O. sinensis to promote quality of life for people with dialysis.


Assuntos
Cordyceps , Diálise Renal , Humanos , Qualidade de Vida , Revisões Sistemáticas como Assunto , Metanálise como Assunto , Literatura de Revisão como Assunto
17.
Food Funct ; 2022 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-36441628

RESUMO

The chemical structure and pharmacological activity of Polygonatum cyrtonema Hua polysaccharides have garnered significant attention in recent years. In this study, a homogeneous polysaccharide, PCP1, was extracted from P. cyrtonema Hua rhizomes and purified. Monosaccharide composition analysis showed that PCP1 is primarily composed of fructose, glucose, and mannose. Chemical structure analysis showed that the main chain of PCP1 is composed mainly of →1)-ß-D-Fruf-(2→ and →1,6)-ß-D-Fruf-(2→, with small amounts of →6)-α-D-Glcp-(1→, →4)-ß-D-Manp-(1→, and ß-D-Glcp-(1→. The side chain is ß-D-Fruf-(2→ linked at C-6 of →1,6)-ß-D-Fruf-(2→. In vivo experiments showed that PCP1 mitigates liver pathological damage, improves abnormal lipid metabolism and oxidative stress, promotes the production of short-chain fatty acids, and balances the composition of the intestinal microbiota in non-alcoholic fatty liver disease (NAFLD) mice. Thus, PCP1 can be used as a natural ingredient in functional foods for the treatment of NAFLD.

18.
Front Aging Neurosci ; 14: 1009632, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36313014

RESUMO

Alzheimer's disease (AD) is a common neurodegenerative disorder causing dementia in the elderly population. Functional disconnection of brain is considered to be the main cause of AD. In this study, we applied a newly developed association (Asso) mapping approach to directly quantify the functional disconnections and to explore the diagnostic effects for AD with resting-state functional magnetic resonance imaging data from 36 AD patients and 42 age-, gender-, and education-matched healthy controls (HC). We found that AD patients showed decreased Asso in left dorsoanterior insula (INS) while increased functional connections of INS with right medial prefrontal cortex (MPFC) and left posterior cingulate cortex (PCC). The changed Asso and functional connections were closely associated with cognitive performances. In addition, the reduced Asso and increased functional connections could serve as effective neuromarkers to distinguish AD patients from HC. Our research provided new evidence for functional disconnections in AD and demonstrated that functional disconnections between cognition-memory networks may be potential early biomarkers for AD.

19.
Front Surg ; 9: 966375, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36303853

RESUMO

Background: Skin innervation plays an important role in wound healing by either direct contact with or indirect secretions that impact skin cells. Many studies in this field have been published; however, there is a lack of bibliometric analyses focusing on the effect of skin innervation on skin wound healing. In this study, we aimed to analyse the research trends, status, and hotspots in this field. Methods: Reviews and articles published in English were extracted from the Web of Science Core Collection (WoSCC) database based on subject term searches. Microsoft Office Excel, VOSviewer, and CiteSpace were used to analyse publication date, country or region, institution, author, and author keywords. Results: A total of 368 papers published between 1959 and 2022 were included in the analysis. Although there was a pulsation during this period, there was an overall upward trend in studies related to the effect of skin innervation on wound healing. The United States, particularly the University of Washington, and Gibran, Nicole S. from the University of Washington, was the most active in this field. Wound Repair and Regeneration published the most relevant literature, and "Calcitonin gene-related peptide: physiology and pathophysiology" had the highest total number of citations. "Diabetic foot ulcer," "epidermal stem cells," "mesenchymal stem cells," and "mast cells" are current and potential future research hotspots. Conclusion: This bibliometric analysis will inform the overall trends in research related to the effect of skin innervation on wound healing, summarise relevant research hotspots, and guide future work.

20.
Pharmaceutics ; 14(10)2022 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-36297592

RESUMO

Cationic liposome delivery of interfering RNA (shRNA) plays an important role in tumor therapy. The cyclic Arg-Gly-Asp (cRGD) modified cationic liposomes (cRGD-CL) were designed for targeted delivery of ONECUT2 (OC-2) shRNA (pshOC-2) to breast cancer cells. The characterization analysis of cationic liposome showed that the prepared cRGD-CL/pshOC-2 lipoplexes had uniform particle size (150 ± 1.02 nm), moderate zeta potential (19.8 ± 0.249 mV) and high encapsulation efficiency (up to 96%). The results of flow cytometer showed that the introduction of cRGD could significantly promote the liposomes targeting tumor cells. In MCF-7 cells, the pshOC-2 could down-regulate expression of OC-2 and result in cell apoptosis, inhibition of the wound healing, migration and cell colony formation, in which the signal pathways of Bcl-xL, Bcl-2 were inhibited and the signal pathways of Bax and Cleaved Caspase-3 were promoted. In MCF-7 xenograft mice, intravenous administration of cRGD-CL/pshOC-2 lipoplexes could effectively reduce the expression of OC-2 in tumors and result in apparently antitumor effects, which suggested that the lipoplexes might be deeply penetrated into tumor through receptor-mediated transcytosis. The results revealed that the cationic liposome (cRGD-CL) was an effective delivery system for OC-2 shRNA, which might be an effective therapeutic candidate for breast cancer.

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