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1.
Sci Rep ; 10(1): 2044, 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-32029792

RESUMO

GPNCA is a long non-coding RNA with unknown functions. In this study, using data from 9 cancers obtained from The Cancer Genome Atlas (TCGA), GPNCA was identified as overexpressed in cancer vs. normal tissues. The upregulation of GPNCA was associated with poor overall prognosis in colon, liver, renal clear cell and breast cancers. The upregulation of GPNCA was partly due to enhanced H3K27ac occupancy on its promoter region via EP300 and KAT2A/GCN5. The overexpression of GPNCA was positively related to tumor metastasis in colon cancer and poor disease-free and recurrence-free survival in colon and liver cancer. Both gene ontology (GO) enrichment and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis indicated that GPNCA was closely linked to regulation of gene transcription and post-transcriptional modifications, which was further supported by in vitro cell cytoplasmic and nuclear RNA purification assessments. Furthermore, GPNCA was associated with cell growth. Our in vitro experiments demonstrated that GPNCA silencing inhibited tumor growth via inhibiting its nearby gene GSK3B. Taken together, these findings highlight GPNCA as a biomarker for cancer diagnosis and a potential target for future cancer drug development.

2.
PLoS One ; 14(3): e0213626, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30865716

RESUMO

Automated cell classification is an important yet a challenging computer vision task with significant benefits to biomedicine. In recent years, there have been several studies attempted to build an artificial intelligence-based cell classifier using label-free cellular images obtained from an optical microscope. Although these studies showed promising results, such classifiers were not able to reflect the biological diversity of different types of cell. While in terms of malignant cell, it is well-known that intracellular actin filaments are altered substantially. This is thought to be closely related to the abnormal growth features of tumor cells, their ability to invade surrounding tissues and also to metastasize. Therefore, being able to classify different types of cell based on their biological behaviors using automated technique is more advantageous. This article reveals the difference in the actin cytoskeleton structures between breast normal and cancer cells, which may provide new information regarding malignant changes and be used as additional diagnostic marker. Since the features cannot be well detected by human eyes, we proposed the application of convolutional neural network (CNN) in cell classification based on actin-labeled fluorescence microscopy images. The CNN was evaluated on a large number of actin-labeled fluorescence microscopy images of one human normal breast epithelial cell line and two types of human breast cancer cell line with different levels of aggressiveness. The study revealed that the CNN performed better in the cell classification task compared to a human expert.


Assuntos
Actinas/química , Neoplasias da Mama/diagnóstico por imagem , Mama/diagnóstico por imagem , Citoesqueleto/química , Reconhecimento Automatizado de Padrão , Algoritmos , Automação , Linhagem Celular , Linhagem Celular Tumoral , Citoplasma , Feminino , Humanos , Processamento de Imagem Assistida por Computador , Células MCF-7 , Aprendizado de Máquina , Microscopia de Fluorescência , Modelos Estatísticos , Reprodutibilidade dos Testes
3.
EBioMedicine ; 41: 200-213, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30796006

RESUMO

BACKGROUND: LncRNAs have been found to be involved in various aspects of biological processes. In this study, we aimed to uncover the molecular mechanisms of lncRNA EPB41L4A-AS1 in regulating glycolysis and glutaminolysis in cancer cells. METHODS: The expression of EPB41L4A-AS1 in cancer patients was analyzed in TCGA and GEO datasets. The level of cellular metabolism was determined by extracellular flux analyzer. The relationship between p53 and EPB41L4A-AS1 was explored by qRT-PCR, luciferase assay and ChIP assay. The interactions between EPB41L4A-AS1 and HDAC2 or NPM1 were determined by RNA immunoprecipitation, RNA pull-down assay and RNA-FISH- immunofluorescence. FINDINGS: EPB41L4A-AS1 was a p53-regulated gene. Low expression and deletion of lncRNA EPB41L4A-AS1 were found in a variety of human cancers and associated with poor prognosis of cancer patients. Knock down EPB41L4A-AS1 expression triggered Warburg effect, demonstrated as increased aerobic glycolysis and glutaminolysis. EPB41L4A-AS1 interacted and colocalized with HDAC2 and NPM1 in nucleolus. Silencing EPB41L4A-AS1 reduced the interaction between HDAC2 and NPM1, released HDAC2 from nucleolus and increased its distribution in nucleoplasm, enhanced HDAC2 occupation on VHL and VDAC1 promoter regions, and finally accelerated glycolysis and glutaminolysis. Depletion of EPB41L4A-AS1 increased the sensitivity of tumor to glutaminase inhibitor in tumor therapy. INTERPRETATION: EPB41L4A-AS1 functions as a repressor of the Warburg effect and plays important roles in metabolic reprogramming of cancer.


Assuntos
Núcleo Celular/metabolismo , Glicólise , Histona Desacetilase 2/metabolismo , Proteínas de Transporte da Membrana Mitocondrial/genética , Neoplasias/metabolismo , RNA Longo não Codificante/genética , Transporte Ativo do Núcleo Celular , Animais , Glutaminase/metabolismo , Células HeLa , Células Hep G2 , Humanos , Camundongos , Camundongos Nus , Proteínas de Transporte da Membrana Mitocondrial/metabolismo , RNA Longo não Codificante/metabolismo
4.
J Orthop Surg Res ; 13(1): 226, 2018 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-30185202

RESUMO

BACKGROUND: To compare the efficacy and safety of K-wire tension band fixation (KTB) with other alternative approaches (cannulated screws, cable pin, and ring pin) for treatment of patella fractures by performing a meta-analysis. METHODS: PubMed and EMBASE databases were searched for all relevant studies. Standardized mean difference (SMD) or relative risk (RR) and their corresponding 95% confidence intervals (CIs) were calculated for continuous or dichotomous outcomes via either a fixed- or random-effect model using Stata 13.0 software. RESULTS: Nine literatures involving 949 patients (581 in the KTB group and 368 in the control group) were included. Pooled analysis showed there were no differences in the success rate, operative time, healing time, and number of infections between patients undergoing KTB and others. However, the incidence of complications (RR = 8.04, 95% CI = 4.45-14.53; p < 0.001) and VAS (SMD = 0.642, 95% CI = 0.22-1.06; p = 0.003) were lower, while flexion degree (SMD = - 0.70 95% CI = - 1.04-- 0.36; p < 0.001), Böstman joint function score (SMD = - 0.68, 95% CI = - 1.10-- 0.27; p = 0.001), Iowa knee score (RR = 0.88, 95% CI = 0.81-0.96; p = 0.004), and Lysholm score (SMD = - 0.71, 95% CI = - 1.10-- 0.32; p < 0.001) were significantly higher in patients undergoing alternative approaches than the KTB. Subgroup analysis also demonstrated the cannulated screw fixation was superior to KTB in reducing the incidence of complications. CONCLUSIONS: Alternative treatments may be effective for management of patella fractures and should be attempted to be popularized in clinic.


Assuntos
Fios Ortopédicos , Fraturas Ósseas , Patela , Idoso , Parafusos Ósseos , Feminino , Fixação Interna de Fraturas , Fraturas Ósseas/cirurgia , Humanos , Masculino , Pessoa de Meia-Idade , Patela/lesões , Resultado do Tratamento
5.
Springerplus ; 5(1): 1613, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27652186

RESUMO

Characteristics of flow field and stirring ability of coherent jet with various shrouding CH4 flow rates on the molten bath were studied by combustion experiment and numerical simulation. The axial velocity and total temperature distributions of coherent jet under hot (1700 K) and cold (298 K) ambient condition were analyzed. The Eddy Dissipation Concept model was used in simulation with detail chemical kinetic mechanisms, and the numerical simulation results were agreed well with the combustion experiment in this research. Based on the simulation and experiment results, when the CH4 rate was 230, 207 and 184 Nm(3)/h, their disparity rate of average velocity and total temperature was small than 5 and 6 %, respectively, at high ambient temperature. Hence, the same stirring effect might be achieved by those three kinds of CH4 flow rates in EAF steelmaking process. According to the industrial application research, the best CH4 flow rate is 184 Nm(3)/h, which could stir molten bath well and reduce energy consumption in steelmaking process.

6.
Zhonghua Wai Ke Za Zhi ; 47(16): 1253-6, 2009 Aug 15.
Artigo em Chinês | MEDLINE | ID: mdl-19781176

RESUMO

OBJECTIVE: To investigate whether or not allografted olfactory mucosa gliacytes could repair peripheral nerve injure. METHODS: Olfactory mucosa gliacytes had been cultured in vitro for 2 weeks, then purified and condensed for later transplantation.Sixty adult female Wistar rats were randomized into 2 groups of 30 rats each, A (control) and B (test). Rats' left sciatic nerves were excised 25 mm long axons and retained epineurium lumen anastomosed to proximal ends. Culture mediums, and olfactory mucosa gliacytes were transplanted into epineurium lumen of A and B groups respectively. At 3 months postoperatively, the regenerations of injured sciatic nerves were evaluated by methods of macroscopy, photomicroscopy, transmission electron microscopy, retro-marked fluorescence red, the condensation of glial fibre acid protein (GFAP) and nerve growth factors (NF) assayed by immunofluorescence, and the concentration of myelin basic protein (MBP) and neurofilament protein (NF) assayed by enzyme linked immunosorbent assay. RESULTS: The regenerations of injured sciatic nerves were superior in B group to in A group; the transportation distance of retro-marked fluorescence red were longer in B group than in A group (P < 0.01). The condensations of GFAP and NGF were more dense in B group than in A group. The concentrations of MBP and NF were more high in B group than in A group (P < 0.01). The function scores of injured limbs were superior in B group to in A group (P < 0.01). The quantifications of nerve fibers and myelin fibers of injured sciatic nerve were larger in B group than in A group (P < 0.01). CONCLUSION: Allografted olfactory mucosa gliacytes could repair injured nerve defect.


Assuntos
Transplante de Células , Neuroglia/citologia , Mucosa Olfatória/citologia , Nervo Isquiático/lesões , Animais , Células Cultivadas , Modelos Animais de Doenças , Feminino , Regeneração Nervosa , Distribuição Aleatória , Ratos , Ratos Wistar , Transplante Homólogo
7.
Artigo em Chinês | MEDLINE | ID: mdl-19192869

RESUMO

OBJECTIVE: To compare their competence of olfactory epithelial gliocytes, olfactory globular nerve layer (OGNL) gliocytes and SC in repair nerve defect of sciatic nerve, and select the best gliocytes for repair of peripheral nerve defect. METHODS: Olfactory epithelial gliocytes, OGNL gliocytes and SC were extracted from 20 female Wistar rats aged 2-3 months and cultured in vitro for 2 weeks, then purified and condensed for transplantation. Eighty adult female Wistar rats were randomized into groups A, B, C and D (n = 20). The left sciatic nerves were excised 25 mm axons and retained epineurium lumen anastomosed to proximal ends. The culture mediums, SC, OGNL gliocytes, and olfactory epithelial gliocytes were transplanted into the epineurium lumen of groups A, B, C and D, respectively. Three months postoperatively, the injured sciatic nerve regeneration was evaluated by methods of macroscopic observation, photomicroscope, transmission electron microscope, retro-marked fluorescence transportation distance, the glial fibrillary acidic protein (GFAP) and nerve growth factor (NGF) were assayed by immunofluorescence, and the myelin basic protein (MBP) and neurofilament (NF) protein were assayed by ELISA. RESULTS: The scores of ankle joint were (3.325 +/- 0.963), (4.200 +/- 1.005), (5.143 +/- 0.635) and (5.950 +/- 0.154) in groups A, B, C and D, respectively; showing statistically significant difference between groups (P < 0.05). The observations of gross, sections under microscope and transmission electron microscope showed the regeneration of defect nerve was best in group D, followed by group C, and group B was superior to group A. The transportation distance of retro-marked fluorescence was longest in group D, followed by group C, and group B was superior to group A. The concentrations of GFAP and NGF were largest in group D, followed by group C, and group B was superior to group A. The MBP concentrations were (9.817 +/- 3.267), (12.347 +/- 3.091), (14.937 +/- 2.075) and (22.757 +/- 0.871) ng/mL in groups A, B, C and D, respectively; showing statistically significant difference between other groups (P < 0.05) except between group A and group B (P > 0.05). And the NF concentrations were (13.869 +/- 5.677), (18.498 +/- 3.889), (23.443 +/- 2.260) and (27.610 +/- 1.125) ng/mL in groups A, B, C and D, respectively; showing statistically significant difference between groups (P < 0.05). CONCLUSION: Olfactory epithelial gliocytes, OGNL gliocytes and SC transplantation could repair injured nerve. The competence of olfactory epitheliums is superior to the OGNL gliocytes and SC, and the OGNL gliocytes is better than SC.


Assuntos
Transplante de Células , Neuroglia/citologia , Nervo Isquiático/cirurgia , Animais , Feminino , Regeneração Nervosa , Mucosa Olfatória/citologia , Nervo Olfatório/citologia , Ratos , Ratos Wistar , Nervo Isquiático/lesões
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