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1.
Water Res ; 168: 115159, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31614234

RESUMO

Worldwide, it is common that the drinking water distribution systems (DWDSs) may be subjected to changes of supply water quality due to the needs of upgrading the treatment processes or switching the source water. However, the potential impacts of quality changed supply water on the stabilized ecological niches within DWDSs and the associated water quality deterioration risks were poorly documented. In the present study, such transition effects caused by changing the supply water quality that resulted from destabilization of biofilm and loose deposits in DWDS were investigated by analyzing the physiochemical and microbiological characteristics of suspended particles before (T0), during (T3-weeks) and after upgrading the treatments (T6-months) in an unchlorinated DWDS in the Netherlands. Our results demonstrated that after 6 months' time the upgraded treatments significantly improved the water quality. Remarkably, water quality deterioration was observed at the initial stage when the quality-improved treated water distributed into the network at T3-weeks, observed as a spike of total suspended solids (TSS, 50-260%), active biomass (ATP, 95-230%) and inorganic elements (e.g. Mn, 130-250%). Furthermore, pyrosequencing results revealed sharp differences in microbial community composition and structure for the bacteria associated with suspended particles between T0 and T3-weeks, which re-stabilized after 6 months at T6-months. The successful capture of transition effects was especially confirmed by the domination of Nitrospira spp. and Polaromonas spp. in the distribution system at T3-weeks, which were detected at rather low relative abundance at treatment plant. Though the transitional effects were captured, this study shows that the introduction of softening and additional filtration did not have an effect on the water quality for the consumer which improved considerably after 6-months' period. The methodology of monitoring suspended particles with MuPFiSs and additional analysis is capable of detecting transitional effects by monitoring the dynamics of suspended particles and its physiochemical and microbiological composition.


Assuntos
Água Potável , Biofilmes , Países Baixos , Microbiologia da Água , Qualidade da Água , Abastecimento de Água
2.
Opt Express ; 27(21): 29817-29828, 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31684238

RESUMO

Optical discrete multi-tone (DMT) is one type of direct-detection optical orthogonal frequency-division multiplexing (DDO-OFDM), and it is more suitable for cost-sensitive access networks and optical interconnections due to its simple structure. In DMT transmitter, inverse fast Fourier transform (IFFT) is an essential function for achieving OFDM modulation, and its input data are constrained to have Hermitian symmetry (HS). To support high-speed DMT signal generation, a fully-parallel implementation of IFFT is preferable. However, the hardware implementation of the conventional complex-valued IFFT (CC-IFFT) requires large area and has high power consumption. Based on the nature of HS, we design and implement a fully-parallel pipelined 128-point radix-2 decimation-in-time Hermitian-symmetric IFFT (HS-IFFT) by using a single field programmable gate array (FPGA) chip. On-chip resource utilization is analyzed for both the proposed HS-IFFT and CC-IFFT. It exhibits that the HS-IFFT can save up to 35% multipliers, 49% registers and 43% look-up tables (LUTs) compared to the CC-IFFT. Also, by using the HS-IFFT and CC-IFFT, two FPGA-based real-time baseband DMT transmitters are implemented and power consumption is estimated. More than 32% of on-chip power is saved by using the HS-IFFT. Moreover, the two DMT transmitters are also experimentally demonstrated in a short-reach directly-modulated laser (DML)-based optical DMT system. The experimental results show that the HS-IFFT-DMT has the same bit error rate (BER) and error vector magnitude (EVM) performances as the CC-IFFT-DMT in both electrical/optical back-to-back cases (EB2B/OB2B) and post 20-km single-mode fiber (SMF) transmission.

3.
Opt Express ; 27(20): 29297-29308, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31684666

RESUMO

We investigate theoretically the generation and enhancement of sum sideband in a quadratically coupled optomechanical system with parametric interactions. It is shown that the generation of frequency components at the sum sideband stems from the nonlinear optomechanical interactions via two-phonon processes in the quadratically coupled optomechanical system, while an optical parametric amplifier (OPA) inside the system can considerably improve the sum sideband generation (SSG). The dependence of SSG on the system parameters, including the power of the control field, the frequency detuning of the probe fields and the nonlinear gain of OPA are analyzed in detail. Our analytic calculation indicates that the SSG can be obtained even under weak driven fields and greatly enhanced via meeting the matching conditions. The effect of SSG may have potential applications for achieving measurement of electric charge (or other weak forces) with higher precision and on-chip manipulation of light propagation.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31718977

RESUMO

Lipid droplets (LDs) are organelles featuring in intracellular storage of neutral lipids, which are involved with many biological processes. Monitoring the dynamical cyclic behaviors of cellular LDs poses great importance for early disease diagnosis. Herein, two coumarin-based LDs-specific fluorescence probes exhibited "turn-on" and two-photon fluorescence triggered by breaking their aggregation states. By virtue of establishing oil/water emulsions model to simulate LDs, the behaviors of "turn-on" fluorescence were elucidated, which benefited for the enhancement of selectivity of the probes to LDs. Then, we highlight a LDs-specific coumarin-based two-photon probe (L1) with high photo-stability to monitor the dynamic cyclic behaviors of LDs in cells, unraveling the changes of LDs quantity during lipophagy and the reproduction of LDs to prevent lipotoxicity. We believe the probe offers a convenient way to investigate the biology of LDs.

5.
Chem Commun (Camb) ; 55(92): 13840-13843, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31670334

RESUMO

A Cp*CoIII-catalyzed arene C-H bond amidation/annulation of benzamides was developed to afford quinazolinone derivatives in one-pot with high yields and broad substrate scope. This method could be applied to the synthesis of quinazolinone drugs and late-stage modification of natural products.

6.
Artigo em Inglês | MEDLINE | ID: mdl-31696975

RESUMO

BACKGROUND AND AIM: Aminosalicylic acids are recognized to be the first-line treatment options for ulcerative colitis. Currently, the effectiveness of curcumin as an adjuvant treatment in ulcerative colitis has been investigated, which was still controversial. This study aimed to systematically review and meta-analyze the efficacy and safety of curcumin as an adjuvant treatment in ulcerative colitis. METHODS: The PubMed, EMBASE, and Cochrane Library databases were searched from original to July 2019, and relevant randomized controlled clinical trials were enrolled and analyzed. The primary outcomes were clinical and endoscopic remission; meanwhile, the secondary outcomes were clinical and endoscopic improvement. Subgroup analyses of doses, delivery way, form, and intervention time of curcumin were also conducted. RESULTS: Six randomized controlled clinical trials with a total of 349 patients were included. Eligible trials suggested that adjuvant curcumin treatment in ulcerative colitis was effective in inducing clinical remission (odds ratio [OR] = 5.18, 95% confidence interval [CI]: 1.84-14.56, P = 0.002), endoscopic remission (OR = 5.69, 95% CI: 1.28-25.27, P = 0.02), and endoscopic improvement (OR = 17.05, 95% CI: 1.30-233.00, P = 0.03), but not in clinical improvement (OR = 4.79, 95% CI: 1.02-22.43, P = 0.05). We can see the potential advantages in large dosage, topical enema, special drug form, and longer duration from the enrolled studies. There were no severe side effects reported. CONCLUSIONS: Curcumin, as an adjuvant treatment of mesalamine, was proved to be effective and safe in ulcerative colitis. Better efficacy can be achieved with suitable dose, delivery way, formation, and intervention time, which needs further study to verify.

7.
Sensors (Basel) ; 19(21)2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31689974

RESUMO

Volatile organic compounds (VOCs) released by plants are closely associated with plant metabolism and can serve as biomarkers for disease diagnosis. Huanglongbing (HLB), also known as citrus greening or yellow shoot disease, is a lethal threat to the multi-billion-dollar citrus industry. Early detection of HLB is vital for removal of susceptible citrus trees and containment of the disease. Gas sensors are applied to monitor the air quality or toxic gases owing to their low-cost fabrication, smooth operation, and possible miniaturization. Here, we report on the development, characterization, and application of electrical biosensor arrays based on single-walled carbon nanotubes (SWNTs) decorated with single-stranded DNA (ssDNA) for the detection of four VOCs-ethylhexanol, linalool, tetradecene, and phenylacetaldehyde-that serve as secondary biomarkers for detection of infected citrus trees during the asymptomatic stage. SWNTs were noncovalently functionalized with ssDNA using π-π interaction between the nucleotide and sidewall of SWNTs. The resulting ssDNA-SWNT hybrid structure and device properties were investigated using Raman spectroscopy, ultraviolet (UV) spectroscopy, and electrical measurements. To monitor changes in the four VOCs, gas biosensor arrays consisting of bare SWNTs before and after being decorated with different ssDNA were employed to determine the different concentrations of the four VOCs. The data was processed using principal component analysis (PCA) and neural net fitting (NNF).

8.
Nanotoxicology ; : 1-19, 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31703536

RESUMO

Mitochondria-associated endoplasmic reticulum (ER) membranes (MAMs) are central microdomains of the ER that interact with mitochondria. MAMs provide an essential platform for crosstalk between the ER and mitochondria and play a critical role in the local transfer of calcium (Ca2+) to maintain cellular functions. Despite the potential uses of superparamagnetic iron oxide nanoparticles (SPIO-NPs) in biomedical applications, the hepatotoxicity of these nanoparticles (NPs) is not well characterized and little is known about the involvement of MAMs in ER-mitochondria crosstalk. We studied SPIO-NPs-associated hepatotoxicity in vitro and in vivo. In vitro, human normal hepatic L02 cells were exposed to SPIO-NPs (2.5, 7.5, and 12.5 µg/mL) for 6 h and SPIO-NPs (12.5 µg/mL) was found to induce apoptosis. In vivo, SPIO-NPs induced liver injury when mice were intravenously injected with 20 mg/kg body weight SPIO-NPs for 24 h. Based on both in vitro and in vivo studies, we found that the structure and Ca2+ transport function of MAMs were perturbated and an accumulation of cyclooxygenase-2 (COX-2) in MAMs fractions was increased upon treatment of SPIO-NPs. The interaction between COX-2 and the components of MAMs, in terms of IP3R-GRP75-VDAC1 complex, was also revealed. Furthermore, the role of COX-2 in SPIO-NPs-associated hepatotoxicity was investigated by modifying the expression of COX-2. We demonstrated that COX-2 increases the structural and functional ER-mitochondria coupling and enhances the efficacy of ER-mitochondria Ca2+ transfer through the MAMs, thus sensitizing hepatocytes to a mitochondrial Ca2+ overload-dependent apoptosis. Taken together, our findings link SPIO-NPs-triggered hepatotoxicity with ER-mitochondria Ca2+ crosstalk which is mediated by COX-2 and provide mechanistic insight into the impact of interorganelle ER-mitochondria communication on hepatic nanotoxicity.

9.
Med Sci Monit ; 25: 8854-8862, 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31756174

RESUMO

BACKGROUND Protease-Activated Receptor 2 (PAR2), a G-protein-coupled receptor, has been proved to be enhanced in human coronary atherosclerosis lesions. We aimed to investigate whether PAR2 actively participates in the atherosclerosis process. MATERIAL AND METHODS PAR2 expression was assessed in blood samples by RT-qPCR from healthy controls and patients with atherosclerosis. Human vascular smooth muscle cells (VSMCs) were treated with oxidative low-density lipoprotein (ox-LDL). After PAR2 overexpression by transfection, cell proliferation was determined by CCK-8, and cell migration was evaluated by Transwell assay. The protein expressions associated with cell growth and migration were measured by Western blot. The distribution of alpha-SMA in VSMCs was evaluated by immunofluorescence. RESULTS Expression of PAR2 was higher in patients with atherosclerosis compared with normal controls. PAR2 mRNA and protein expression was increased in ox-LDL-treated VSMCs compared with control cells. Induced overexpression of PAR2 in VSMCs led to a reduction in alpha-SMA expression compared to controls. In addition, PAR2 overexpression caused increased migration compared to normal controls, and upregulated MMP9 and MMP14 expression. PAR-2 overexpression promoted cell proliferation compared to control cells, and increased expression levels of CDK2, and CyclinE1, but reduced levels of p27. We preliminary explored the potential mechanism of PAR2, and results showed that overexpression of PAR2 increased expression levels of VEGFA and Angiopoietin 2 compared to controls. Moreover, overexpression of PAR2 enhanced production of tissue factor and IL-8 compared to normal controls. CONCLUSIONS PAR2 promotes cell proliferation and disrupts the quiescent condition of VSMCs, which may be a potential therapeutic target for atherosclerosis.

10.
Br J Pharmacol ; 2019 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-31758704

RESUMO

BACKGROUND AND PURPOSE: The kidney is essential in regulating sodium homeostasis and BP. The irreversible oxidation of cysteine 674 (C674) in the sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2) is increased in the renal cortex of hypertensive mice. Whether inactivation of C674 promotes hypertension is unclear. This study was to investigate whether the inactivation of C674 affected BP and its role in the kidney. EXPERIMENTAL APPROACH: We used heterozygous SERCA2 C674S knock-in (SKI) mice that half of C674 was substituted by S674 to represent partial irreversible oxidation of C674. The BP, urine volume and urine composition of SKI mice and their littermate wild type (WT) mice were measured. The kidneys were collected for cell culture, Na+ /K+ -ATPase activity, protein expression, and immunohistological analysis. KEY RESULTS: compared with WT mice, SKI mice had higher BP, lower urine volume and sodium excretion, upregulated endoplasmic reticulum (ER) stress markers and soluble epoxide hydrolase (sEH), and downregulated dopamine D1 receptor (D1R) in renal cortex and cells from renal proximal tubule (RPT). ER stress and sEH were mutually regulated, and both in the upstream of D1R. Inhibition of ER stress or sEH could upregulate the expression of D1R, decrease the activity of Na+ /K+ -ATPase, increase sodium excretion, and lower BP in SKI mice. CONCLUSIONS AND IMPLICATIONS: The inactivation of SERCA2 C674 promotes the development of hypertension by inducing ER stress and sEH. Our study highlights the importance of C674 redox status in BP control and the contribution of SERCA2 to sodium homeostasis and BP in the kidney.

11.
Int J Biol Macromol ; 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31726176

RESUMO

Polysaccharides are considered to be promising candidates for non-viral gene delivery because of their molecular diversity, which can be modified to fine-tune their physicochemical properties. In this work, transcriptional activator protein (TAT) functionalized PEI grafted polysaccharide polymer (PRBP) was prepared by using rice bran polysaccharide as the starting material, and characterized by various methods. The potential of TAT functionalized PRBP (PRBP-TAT) as gene vector was studied in vitro, including DNA loading capacity, DNA protection ability and biocompatibility. The cell uptake and transfection efficiency of the PRBP-TAT/pDNA polyplexes were studied. The results showed that PRBP-TAT could completely condense DNA at N/P 2. The PRBP-TAT/pDNA polyplexes could protect DNA from degrading by DNase and were efficiently internalized by cells. Biocompatibility result showed that PRBP-TAT had no significant cytotoxicity and effect on cell proliferation. At low N/P ratios of 1-3.5, PRBP-TAT showed higher transfection efficiency than PEI30k and PEI30k-grafted rice bran polysaccharide. PRBP-TAT and PEI showed the highest transfection efficiency of 42.8% and 28.1% when pDNA is 2 µg and N/P ratio is 1.5, respectively, while PRBP showed the highest transfection efficiency of 37.3% at N/P 2.5. These results indicate that PTA is a promising candidate vector for safe and efficient gene delivery.

12.
Int Immunopharmacol ; 77: 105964, 2019 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-31669889

RESUMO

Acute lung injury (ALI) and its most severe form acute respiratory distress syndrome (ARDS) caused by gram-positive bacteria threatens human life because effective treatments and medicines is unavailable. Protostemonine (PSN), an active alkaloid mainly isolated from the roots of Stemona sesslifolia, has anti-inflammatory effects on asthma and gram-negative bacteria-induced ALI. Here, we found that PSN exhibits anti-inflammatory effects and alleviates heat-killed methicillin-resistant Staphylococcus aureus (HKMRSA)-induced pneumonia. PSN treatment significantly attenuated HKMRSA-induced pathological injury, pulmonary neutrophil infiltration, tissue permeability and the production of pro-inflammatory cytokines (TNF-α, IL-1ß and IL-6) in murine ALI model. In addition, PSN decreased the content of TNF-α, IL-1ß, IL-6 and the expression of iNOS, as well as the production of NO in HKMRSA-induced bone marrow derived macrophages (BMDMs). Furthermore, treatment with PSN suppressed the activation of MAPKs (e.g. p38 MAPK, JNK and ERK) and NF-κB. Collectively, our results suggest that PSN ameliorates gram-positive bacteria-induced ALI in mice by inhibition of the MAPK and NF-κB signaling pathways, and our studies suggest that PSN might be a novel candidate for treating ALI/ARDS.

13.
BMC Med Genet ; 20(1): 180, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31727011

RESUMO

BACKGROUND: Duchenne muscular dystrophy (DMD) is a severe X-linked recessive neuromuscular disorder. Patients with DMD usually have severe and fatal symptoms, including progressive irreversible muscle weakness and atrophy complicated with gastrocnemius muscle pseudohypertrophy. DMD is caused by mutations in the dystrophin-encoding DMD gene, including large rearrangements and point mutations. This retrospective study was aimed at supplying information on our 4-year clinical experience of DMD genetic and prenatal diagnosis at the Department of Prenatal Diagnosis in Women's Hospital of Nanjing Medical University. METHODS: Multiplex ligation-dependent probe amplification (MLPA) was used to detect the exon deletions or duplications. And Ion AmpliSeq™ panel for inherited disease was used as the next-generation sequencing (NGS) method to identify the point mutations in exons of DMD gene, but the introns were not sequenced. RESULTS: In this study, the large deletions and duplications of DMD gene were detected in 32 (51.6%) of the 62 families, while point mutations were detected in 20 families (32.3%). The remaining 10 families with a negative genetic diagnosis need to be reevaluated for clinical symptoms or be detected by other molecular methods. Notably, six novel mutations were identified, including c.412A > T(p.Lys138*), c.2962delT(p.Ser988Leufs*16), c.6850dupA (p.Ser2284Lysfs*7), c.5139dupA (p.Glu 1714Argfs*5), c.6201_6203delGCCins CCCA(p.Val2069Cysfs*14) and c.10705A > T (p.Lys3569*). In 52 families with positive results, 45 mothers (86.5%) showed positive results during carrier testing and de novo mutations arose in 7 probands. The prenatal diagnosis was offered to 34 fetuses whether the pregnant mother was a carrier or not. As a result, eight male fetuses were affected, three female fetuses were carriers, and the remaining fetuses had no pathogenic mutation. CONCLUSIONS: This study reported that MLPA and NGS could be used for screening the DMD gene mutations. Furthermore, the stepwise procedure of prenatal diagnosis of DMD gene was shown in our study, which is important for assessing the mutation type of fetuses and providing perinatal care in DMD high-risk families.

14.
Anal Chem ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31746200

RESUMO

Multiblock DNA probe attracted a large amount of scientific attention, for the development of multi-target biosensor and improved specificity/sensitivity. However, the development of multiblock DNA probes highly relied on the chemical synthesis of organic linkers or nanomaterials, which limited their practicability and biological compatibility. In this work, we developed a label-free assembling strategy using a triblock DNA capture probe, which connects 2 DNA probes with its intrinsic polyA fragment (Probe-PolyA-Probe, PAP). The middle polyA segment has a high affinity to the gold electrode surface, leading to excellent reproducibility, stability, and regeneration of our biosensor. Two flanking capture probes were tandemly co-assembled on the electrode surface with consist spatial relationship and exactly the same amount. When combined with the target DNA, the hybridization stability was improved due to the strong base stacking effect of two capture probes. The sensitivity of our biosensor was proved to be 10 fM, with a wide analysis range between 10 fM to 1 nM. Our PAP-based biosensor showed excellent specificity when facing mismatched DNA sequences. Even single nucleotide polymorphisms (SNP) can be distinguished by each probe. The excellent practicability of our biosensor was demonstrated by analyzing genomic DNA both with and without PCR amplification.

15.
Phys Chem Chem Phys ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31746866

RESUMO

We study the lattice thermal conductivity of two-dimensional (2D) pentagonal systems, such as penta-silicene and penta-germanene. Penta-silicene has been recently reported, while the stable penta-germanene, made up of another group IV element, is first revealed by our ab initio calculations. We find that both penta-silicene and penta-germanene at room temperature have ultra-low lattice thermal conductivities, κ, of 1.29 W m-1 K-1 and 0.30 W m-1 K-1, respectively. To the best of our knowledge, penta-germanene may have the lowest κ in 2D crystal materials. We attribute the ultra-low κ to the weak phonon harmonic interaction and strong anharmonic scattering. A small phonon group velocity, a small Debye frequency, a large Grüneisen parameter, and a large number of modes available for phonon-phonon interplay together lead to the ultra-low κ of penta-silicene and penta-germanene. These discoveries provide new insight into the manipulation of ultra-low κ in 2D materials and highlight the potential applications of silicon and germanium based high thermoelectric materials.

16.
Spectrochim Acta A Mol Biomol Spectrosc ; 227: 117581, 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31670044

RESUMO

A novel multifunctional diarylethene fluorescence sensor 1O containing pyrene unit was designed and synthesized. The photochromism and fluorescence photoswitching properties of this diarylethene were studied in detail by irradiation of UV/Vis lights and response of metal ions in acetonitrile solution. Diarylethene fluorescence sensor 1O has high selectivity and sensitivity for the detections of Cd2+ and Zn2+. The limit of detections (LODs) for Cd2+ and Zn2+ were determined to be 1.85 × 10-9 mol L-1 and 7.68 × 10-9 mol L-1, respectively. The binding constants (Ka) of 1O with Cd2+ and Zn2+ in acetonitrile solution were calculated to be 5.8 × 104 mol-1 L and 6.0 × 104 mol-1 L, respectively. The compound 1O responded to the metal ions (Cd2+/Zn2+) to form complexations with 1 : 1 stoichiometry which were verified by Job's plot and MS analysis, respectively. In addition, the fluorescence sensor 1O has been successfully applied to the detection of Cd2+ and Zn2+ in real water samples and processed into test strips for on-site analysis and testing.

17.
Appl Opt ; 58(27): 7367-7374, 2019 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-31674382

RESUMO

Division of focal plane (DoFP) polarization imaging sensors have the distinct advantage of acquiring temporally synchronized Stokes vector in one scene. The sensors' spatially modulated arrangement of a micropolarization array results in loss of spatial resolution and instantaneous field-of-overview errors. Polarization demosaicking (PDM) methods are often utilized to address these drawbacks and achieve the goal of recovering missing polarization information. In this paper, we propose minimized Laplacian polarization residual interpolation for PDM. The Laplacian energy is introduced to improve the interpolation accuracy. We employ interchannel correlation and a guided filter to generate precise tentative estimates and the interpolation performed in the residual domain, where the residuals are the differences between observed values and tentative estimates. Experiments demonstrate that the proposed algorithm provides superior performance in terms of mean average error and peak signal-to-noise ratio.

18.
Anal Chem ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31692338

RESUMO

Dynamic tracking of the spatiotemporal coordination among various organelles to in-depth understanding of the mechanism of autophagy have attracted considerable attention. However, the monitor of nucleoli participation in autophagy was somehow neglected. Herein, we report a RNA-targeted bioprobe (ADAP) with high selective permeability into nuclear pore complexes, which induced a two-photon (TP) fluorescence "off-on" response by groove combination with RNA, dynamically monitoring the autophagy process among multiorganelles (nucleoli, mitochondria, and mitochondria-containing lysosomes). This work provides a simple and convenient way to observe the dynamic behavior of multiorganelles during the autophagy process, which benefits the understanding of cellular metabolism.

19.
Medicine (Baltimore) ; 98(47): e18019, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31764817

RESUMO

We aimed to investigate the correlation of homocysteine (Hcy) level with clinical characteristics, and explore its predictive value for major adverse cardiovascular events (MACE) risk in female patients with premature acute coronary syndrome (ACS).The serum Hcy level was detected from 1299 female patients with premature ACS. According to the tertile of Hcy level, patients were divided into 3 groups: lowest tertile group (≤9.1 µmol/L), middle tertile group (9.2-11.6 µmol/L) and highest tertile group (>11.6 µmol/L). MACE incidence was recorded and MACE-free survival was caculated with the median follow-up duration of 28.3 months.Increased Hcy correlated with older age (P < .001), higher creatinine level (P < .001), and enhanced uric acid level (P = .001), while reduced fasting glucose concentration (P < .001). MACE incidence was 10.7% and it was highest in highest tertile group (22.1%), followed by middle tertile group (7.7%) and lowest tertile group (2.4%) (P < .001). Receiver operating characteristic curve showed that Hcy distinguished MACE patients from non-MACE patients with the area under the curve of 0.789 (95% CI: 0.742-0.835). Kaplan-Meier curves revealed that MACE-free survival was shortest in Hcy highest tertile group, followed by middle tertile group and lowest tertile group (P < .001). Multivariate Cox analyses further showed that higher Hcy level was an independent predictive factor for poor MACE-free survival (middle tertile vs lowest tertile (P = .001, HR: 3.615, 95% CI: 1.661-7.864); highest tertile vs lowest tertile (P < .001, HR: 11.023, 95% CI: 5.356-22.684)).Hcy serves as a potential predictive factor for increased MACE risk in female patients with premature ACS.

20.
Microb Drug Resist ; 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31755810

RESUMO

Both mcr-1 phosphoethanolamine transferase enzymes and extended-spectrum ß-lactamases (ESBLs) are the main plasmid-mediated mechanisms of resistance to colistin and third-generation cephalosporins, respectively, and currently considered a major concern to humans and food animals. Prevalence of mcr-1 gene in Escherichia coli from dairy cattle has rarely been reported. Our objective was to determine prevalence and characteristics of mcr-1 carrying E. coli isolated from clinical mastitis cases in large dairy farms (>500 cows) in 16 provinces of China. A total of 249 E. coli was isolated from 2,038 mastitic milk samples. Among these isolates, 2.0% (n = 5) and 19.7% (n = 49) were colistin resistant mcr-1-positive and ESBL-producing isolates, respectively. All mcr-1-positive isolates that produced ESBLs also carried the blaCTX-M-15 gene and belonged to phylogroup-A. Most mcr-1 and blaCTX-M-15 genes were located on conjugative plasmids (IncP and IncF, respectively) that were successfully transferred to transconjugants in conjugation experiments. All mcr-1-positive E. coli isolates were multidrug resistant, exhibiting resistance to common antimicrobials. Multilocus sequence typing of these mcr-1-carrying E. coli isolates revealed four sequence types, reflecting substantial diversity. Multilocus sequence analysis detected evolutionary connection of mcr-1 carrying isolates with our recently reported ESBL-producing E. coli isolates, raising concerns regarding fast dissemination between bacteria. To our knowledge, this was the first nation-wide report describing isolates of E. coli from mastitic milk samples collected on large dairy farms in China, carrying mcr-1 and blaCTX-M-15 genes on conjugative plasmids. We concluded that dairy cattle are a potential source of mcr-1-carrying and ESBL-producing E. coli.

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