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1.
Clin Chim Acta ; 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31654632

RESUMO

Myhre syndrome is a rare autosomal dominant multi-organ disorder characterized by growth retardation, skeletal anomalies, muscular hypertrophy, joint stiffness, facial dysmorphism, deafness, cardiovascular disease, and abnormal sexual development. Here we described the first two Chinese Myhre syndrome patients diagnosed by whole-exome sequencing. They both had de novo c.1498A > G (p.Ile500Val) variant in SMAD4 and presented with key characteristics of Myhre syndrome but also revealed uncommon features (polydactyly in the girl and precocious puberty in the boy). We performed functional analysis on four previously reported SMAD4 pathogenic variants in Myhre syndrome patients using dual-luciferase assay. Our results revealed that the pathogenic variants resulted in a variable degree of increased transcription activity of target genes that contain the minimal SMAD binding elements in their promoter regions. The boy responded to the recombinant human growth hormone treatment with improved height but also led to hyperinsulinemia and advanced bone age. Because of his precocious puberty, we subsequently combined the recombinant human growth hormone and gonadotrophin-releasing hormone agonist treatments, which resulted in overall improved height. We reviewed the sexual features of reported Myhre syndrome cases and discussed the possible mechanism of SMAD4 variants in Myhre syndrome that lead to the abnormal hypothalamic-pituitary-gonadal axis.

2.
Child Obes ; 15(7): 459-467, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31408357

RESUMO

Background: Despite perceiving their child as being above a healthy weight, many parents do not intervene. Little is known about the factors influencing parental action. We assessed parental perception of child's weight status, the prevalence of mitigating parental action, and the underlying factors. Methods: We studied 20,242 children and adolescents from 6 centers across China. Anthropometry was measured by research nurses. Parents answered questionnaires, including their perception of their child's weight status, and any subsequent weight treatment. Results: A total of 3254 children had obesity (16.1%), with 63.0% correctly perceived as overweight by their parents. These children were more likely to be older (≥8 years; p < 0.0001), have severe obesity [adjusted relative risk (aRR) 1.41; p < 0.0001], and have mothers with overweight/obesity (aRR 1.15; p < 0.0001). In particular, parents of children aged <8 years were over five times more likely to perceive their child with overweight/obesity as "thin" than parents of teenagers. Conversely, girls, older children/adolescents, and urban youth were more likely to be wrongly perceived by parents as having an overweight issue. Only one in four children (27.8%) with available information received treatment for their perceived weight problem. Children with severe obesity were more likely to be treated (aRR 1.34; p < 0.0001), as were children of mothers with overweight/obesity (aRR 1.18; p = 0.002). Conclusions: Only one in four Chinese children perceived as overweight by their parents received treatment for their weight problem. Given that overweight/obesity in childhood tracks into adulthood and many parents did not intervene despite perceiving an overweight problem in their child, interventions for childhood obesity need to extend beyond parental perception of children's weight status.

3.
BMC Pediatr ; 19(1): 264, 2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31362719

RESUMO

BACKGROUND: The relationship between birth weight and blood pressure has not been well explored in Chinese children and adolescents. The aim of this study was to investigate the relationship between birth weight and childhood blood pressure in China. METHODS: A total of 15324 children and adolescents (7919 boys and 7405 girls) aged 7-17 years were stratified into six birth weight groups. Analysis of covariance and binary logistic regression were used to analyse the relationship between birth weight and blood pressure while controlling for potential confounding factors, including age, gestational age, season of birth and area of residence. RESULTS: The group with birth weights from 2500 to 2999 g had the lowest prevalence of hypertension (8.9%). Lower birth weight children (< 2000 g) had significantly higher systolic blood pressure (SBP) (106.00 ± 0.72, P = 0.017), and children with heavier birth weights also had higher SBP (3500-3999 g, 105.13 ± 0.17, P < .001; ≥ 4000 g, 105.96 ± 0.27, P < .001). No significant relationship was found between birth weight and diastolic blood pressure (DBP). The overall rate of hypertension was 10.8% (12.1% in boys and 9.4% in girls). The median weight group (2500-2999 g) had the lowest rate of hypertension (8.9%). Compared with children in the median weight group, children with lower birth weight had a higher prevalence of hypertension (< 2000 g, OR = 1.85, 95% CI = 1.25-2.74; 2000-2499 g, OR = 1.57, 95% CI = 1.15-2.13), and groups with higher birth weights also had higher risks of hypertension (3500-3999 g, OR = 1.22, 95% CI = 1.02-1.45; ≥ 4000 g, OR = 1.42, 95% CI = 1.16-1.74). CONCLUSIONS: Excluding the confounding effect of obesity, a U-shaped relationship between birth weight and risk of hypertension was found in children and adolescents in Chinese cities. Birth weight significantly influences SBP but has a minimal effect on DBP. Further basic research on foetal development and programming may shed light on this phenomenon.

4.
Pediatrics ; 144(1)2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31253739

RESUMO

OBJECTIVES: Although it is widely believed that China is facing a major shortage of pediatricians, the real situation of the current national status of pediatric human resources and their working conditions has not been evaluated to date. METHODS: We administered a survey to 54 214 hospitals from all 31 provinces in mainland China from 2015 to 2016. Hospital directors of all secondary and tertiary hospitals with pediatric services and a random sample (10%) of primary hospitals provided information on number of pediatricians and their educational levels, specialties, workloads, dropout rates, and other hospital characteristics. A data set of medical resources and socioeconomic information regarding each region (1997-2016) was constructed from the Chinese National Statistics Bureau. The Gini coefficient was used to describe the geographical distributions of pediatricians and hospitals. RESULTS: There were 135 524 pediatricians in China or ∼4 pediatricians per 10 000 children. Pediatricians' average educational level was low, with ∼32% having only 3 years of junior college training after high school. The distribution of pediatricians was extremely skewed (Gini coefficient 0.61), and the imbalance of highly educated pediatricians was even more skewed (Gini coefficient 0.68). The dropout rate of pediatricians was 12.6%. Despite an increase in the Chinese government's financial investment in health over the last decade, physicians have been burdened with a greater workload. CONCLUSIONS: Uneven development of the pediatric care system, inadequately trained pediatricians, low job satisfaction, and unmet demand for pediatric care are the major challenges facing China's pediatric health care system.

5.
Sci Rep ; 9(1): 5281, 2019 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-30918291

RESUMO

Gastric cancer (GC) is the fourth most common malignant neoplasm and the second leading cause of cancer death. Identification of key molecular signaling pathways involved in gastric carcinogenesis and progression facilitates early GC diagnosis and the development of targeted therapies for advanced GC patients. Emerging evidence has revealed a close correlation between forkhead box (FOX) proteins and cancer development. However, the prognostic significance of forkhead box S1 (FOXS1) in patients with GC and the function of FOXS1 in GC progression remain undefined. In this study, we found that upregulation of FOXS1 was frequently detected in GC tissues and strongly correlated with an aggressive phenotype and poor prognosis. Functional assays confirmed that FOXS1 knockdown suppressed cell proliferation and colony numbers, with induction of cell arrest in the G0/G1 phase of the cell cycle, whereas forced expression of FOXS1 had the opposite effect. Additionally, forced expression of FOXS1 accelerated tumor growth in vivo and increased cell migration and invasion through promoting epithelial-mesenchymal transition (EMT) both in vitro and in vivo. Mechanistically, the core promoter region of FOXS1 was identified at nucleotides -660~ +1, and NFKB1 indirectly bind the motif on FOXS1 promoters and inhibit FOXS1 expression. Gene set enrichment analysis revealed that the FOXS1 gene was most abundantly enriched in the hedgehog signaling pathway and that GLI1 expression was significantly correlated with FOXS1 expression in GC. GLI1 directly bound to the promoter motif of FOXS1 and significantly decreased FOXS1 expression. Finally, we found that miR-125a-5p repressed FOXS1 expression at the translational level by binding to the 3' untranslated region (UTR) of FOXS1. Together, these results suggest that FOXS1 can promote GC development and could be exploited as a diagnostic and prognostic biomarker for GC.

6.
Gene ; 689: 11-17, 2019 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-30553996

RESUMO

Most cancer cells predominantly produce their energy through a high rate of glycolysis in the presence of abundant oxygen. Glycolysis has become a target of anticancer strategies. Previous researches showed that glucose transporter 1 (GLUT1) inhibitor is effective as anticancer agents. This study assessed the effects of the selective GLUT1 inhibitor WZB117 on regulation of neuroblastoma (NB) cell line SH-SY5Y viability, cell cycle and glycolysis in vitro. SH-SY5Y cells were grown and treated with WZB117 for up to 72 h and then subjected to cell viability, qRT-PCR, Western blot and flow cytometry analysis. Level of ATP and LDH was also analyzed. The result showed that WZB117 treatment reduced tumor cells viability, downregulated level of GLUT1 protein. Moreover, WZB117 treatment arrested tumor cells at the G0-G1 phase of the cell cycle, induced tumor cells to undergo necrosis instead of apoptosis. In addition, WZB117 treatment downregulated the levels of intracellular ATP, LDH and glycolytic enzymes. Thus, WZB117-induced GLUT1 inhibition suppressed tumor cell growth, induced cell cycle arrest and reduced glycolysis metabolites in NB cells in vitro. This study suggested that GLUT1 can be used as a potential therapeutic target for NB.


Assuntos
Transportador de Glucose Tipo 1/antagonistas & inibidores , Hidroxibenzoatos/farmacologia , Neuroblastoma/metabolismo , Neuroblastoma/patologia , Apoptose/efeitos dos fármacos , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Pontos de Checagem do Ciclo Celular/genética , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Proliferação de Células/genética , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/genética , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Transportador de Glucose Tipo 1/genética , Glicólise/efeitos dos fármacos , Glicólise/genética , Humanos , Neuroblastoma/genética
7.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 34(11): 994-999, 2018 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-30591108

RESUMO

Objective To study the effect of inhibiting the expression of glucose transporter 1 (GLUT1) in neuroblastoma cells on the proliferation, invasion and migration of tumor cells. Methods The specific small molecule inhibitor WZB117 was used to inhibit the expression of GLUT1 in neuroblastoma cells. The expression of GLUT1 mRNA was detected by real-time quantitative PCR; the expression of GLUT1 protein was detected by Western blotting; the ability of cell proliferation was detected by CCK-8 assay; the ability of cell invasion and migration were detected by TranswellTM invasion and migration assay, respectively. Results After treated with WZB117, the expression level of GLUT1 mRNA increased, while the protein expression level decreased in the neuroblastoma cells. Cell proliferation was inhibited, and the ability of cell invasion and migration were reduced. Conclusion Inhibiting the expression of GLUT1 in neuroblastoma cells might attenuate the malignant biological behaviors of tumor cells.


Assuntos
Movimento Celular , Transportador de Glucose Tipo 1/metabolismo , Neuroblastoma/patologia , Linhagem Celular Tumoral , Proliferação de Células , Humanos , Invasividade Neoplásica , RNA Mensageiro
8.
Int J Mol Sci ; 19(11)2018 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-30453504

RESUMO

The assembly and maintenance of cilia depend on intraflagellar transport (IFT) proteins, which play an important role in development and homeostasis. IFT80 is a newly defined IFT protein and partial mutation of IFT80 in humans causes diseases such as Jeune asphyxiating thoracic dystrophy (JATD) and short rib polydactyly (SRP) type III, both characterized by abnormal skeletal development. However, the role and mechanism of IFT80 in the invasion of gastric cancer is unknown. We established SGC-7901 and MKN-45 gastric cancer cell lines that stably overexpressed IFT80, as verified by quantitative reverse transcription-PCR, Western blot, and immunofluorescence. Matrix metalloproteinase-9 (MMP9) plays an important role in tumor invasion, and its expression was assessed by quantitative reverse transcription-PCR, Western blotting, and immunofluorescence. The invasion ability of IFT80 on SGC-7901 and MKN-45 cells was examined by the Matrigel invasion assay. The relationship between p75NGFR, and the p75NGFR antagonists, PD90780 and IFT80, were detected by quantitative reverse transcription-PCR and Western blotting. We first detected an IFT80 expression pattern, and found that IFT80 was highly expressed in gastric cancer clinical samples. Overexpression of IFT80 in the gastric cancer cell lines, SGC-7901 and MKN-45, led to lengthening cilia. Additionally, overexpression of IFT80 significantly improved proliferation and invasion, but inhibited apoptosis, in gastric cancer cells. We further found that overexpression of IFT80 increased p75NGFR and MMP9 mRNA and protein expression. Treatment with the p75NGFR antagonist PD90780 inhibited the increased invasion ability resulting from overexpression of IFT80 in SGC-7901 and MKN-45 gastric cancer cells. Thus, these results suggest that IFT80 plays an important role in invasion of gastric cancer through regulating the ift80/p75NGFR/MMP9 signal pathways.


Assuntos
Proteínas de Transporte/metabolismo , Metaloproteinase 9 da Matriz/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Receptores de Fator de Crescimento Neural/metabolismo , Transdução de Sinais , Neoplasias Gástricas/metabolismo , Neoplasias Gástricas/patologia , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Cílios/metabolismo , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Metaloproteinase 9 da Matriz/genética , Invasividade Neoplásica , Proteínas do Tecido Nervoso/antagonistas & inibidores , Proteínas do Tecido Nervoso/genética , Quinazolinas/farmacologia , Receptores de Fator de Crescimento Neural/antagonistas & inibidores , Receptores de Fator de Crescimento Neural/genética , Transdução de Sinais/efeitos dos fármacos , Neoplasias Gástricas/genética
9.
Onco Targets Ther ; 11: 6925-6935, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30410350

RESUMO

Introduction: Cervical cancer, one of the most common malignant gynecological tumors, is a significant burden on the health of females worldwide. The purpose of this study was to investigate genes associated with lymph node metastasis in cervical cancer. Methods: We report on the lymph node metastasis-associated gene, small cell adhesion glycoprotein (SMAGP), as a key regulator of cervical cancer development and progression. SMAGP expression levels were investigated in 70 cervical squamous cell carcinoma samples and 10 normal cervical squamous epithelium samples. Results: Immunohistochemistry analysis revealed that SMAGP protein levels were significantly elevated in cervical cancer tissue compared with normal cervical squamous epithelium. Silencing of SMAGP induced cell cycle arrest, inhibited the cell proliferation and colony formation ability of cervical cancer cells in vitro and suppressed their tumorigenic potential in nude mice. In addition, SMAGP knockdown reduced expression of epithelial mesenchymal transition-related proteins, including vimentin, ß-cadherin, MMP2, and Twist. Conclusion: Together, our findings demonstrate that SMAGP plays a critical role in cell proliferation and tumorigenesis and could be a new therapeutic target in cervical cancer.

10.
Cell Death Dis ; 9(2): 198, 2018 02 07.
Artigo em Inglês | MEDLINE | ID: mdl-29416014

RESUMO

The purpose of this study was to develop an lncRNA signature to improve the prediction of the prognosis of cervical cancer through integration bioinformatics and analysis of TCGA RNA sequencing data. In this study, we established a set of four lncRNA signatures that was significantly associated with recurrence-free survival using the Cox regression model. Functionally, we screened the CC-associated lncRNA NCK1-AS1 as a new candidate lncRNA and regulator which promotes development and progression in CC. qRT-PCR and RNA in situ hybridization (RISH) results showed that NCK1-AS1 was significantly up-regulated in 77.4% (24/31) of the CC tissue group compared with the normal group (P < 0.01). Interestingly, we demonstrated that transcription factor SP1 directly binds to the promoter to activate NCK1-AS1 expression in SiHa cells. In vitro and in vivo assays of silencing NCK1-AS1 significantly inhibited cell proliferation and invasion, with induction of cell arrest in S phase of the cell cycle. Furthermore, Human Transcriptome Array 2.0 analysis after NCK1-AS1 silencing highlighted alterations in cell proliferation and cell cycle pathways. NCK1-AS1 functioned as a molecular sponge for miR-6857, antagonizing its ability to repress CDK1/6 protein translation. In conclusion, these findings suggest that NCK1-AS1/miR-6857/CDK1 crosstalk serve as a critical effector in cervical cancer progression and may serve as a potential target in cervical cancer.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/genética , Proteína Quinase CDC2/metabolismo , Proteínas Oncogênicas/genética , RNA Antissenso/metabolismo , RNA Longo não Codificante/metabolismo , Neoplasias do Colo do Útero/metabolismo , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Animais , Proteína Quinase CDC2/genética , Ciclo Celular/fisiologia , Linhagem Celular Tumoral , Proliferação de Células/fisiologia , Progressão da Doença , Feminino , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Pessoa de Meia-Idade , Proteínas Oncogênicas/metabolismo , Regiões Promotoras Genéticas , RNA Antissenso/genética , RNA Longo não Codificante/genética , Transfecção , Regulação para Cima , Neoplasias do Colo do Útero/genética , Neoplasias do Colo do Útero/patologia
11.
Obes Res Clin Pract ; 12(Suppl 2): 90-100, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-28111084

RESUMO

Metabolic disorders usually increase the level of reactive oxygen species (ROS) and damage mitochondrial function. The placenta supplies nutrients and hormonal signals to the fetus for regulating fetal metabolism, and is also prone to injury by oxidants. The aim of this study was to determine the effect of pre-existing maternal type 2 diabetes mellitus (DM) combined with obesity on placental mitochondrial function and metabolism disorders of offspring. The study included 96 pregnant women. The women were put into the following groups: healthy women (control, n=24), women with DM (DM, n=24), women with obesity (OB, n=24) and women with both DM and obesity (DM+OB, n=24). The ROS level, mitochondrial content, and the mitochondrial respiratory complex activities of the placenta were measured in the four groups. The expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) was detected by immunofluorescence staining and western blotting. In addition, serum levels of insulin, glucose, leptin, nonesterified fatty acid (NEFA), adiponectin and triglycerides of their offspring were also measured. Maternal DM combined with obesity markedly increased ROS level, reduced mitochondrial DNA (mtDNA) content and mitochondrial respiratory complex I, II-III activities in placenta compared to the placenta from the control group and the DM group. Maternal DM combined with obesity significantly decreased Nrf2 and HO-1 expression. Furthermore, maternal DM combined with obesity influenced the glucose and lipid metabolism in their offspring. In conclusion, women with both DM and obesity detrimentally alter placenta function in oxidative stress regulation, and the Nrf2/ARE (antioxidant responsive element) pathway is involved. This may increase metabolic disturbance susceptibility in their offspring.


Assuntos
Elementos de Resposta Antioxidante/genética , Diabetes Mellitus Tipo 2/complicações , Fator 2 Relacionado a NF-E2/fisiologia , Obesidade/complicações , Placenta/metabolismo , Gravidez em Diabéticas/fisiopatologia , Efeitos Tardios da Exposição Pré-Natal , Adulto , Elementos de Resposta Antioxidante/efeitos dos fármacos , Diabetes Mellitus Tipo 2/metabolismo , Diabetes Mellitus Tipo 2/fisiopatologia , Feminino , Regulação da Expressão Gênica no Desenvolvimento , Humanos , Recém-Nascido , Dinâmica Mitocondrial/fisiologia , Obesidade/metabolismo , Obesidade/fisiopatologia , Estresse Oxidativo , Gravidez , Gravidez em Diabéticas/metabolismo , Espécies Reativas de Oxigênio/metabolismo
12.
Exp Ther Med ; 13(3): 1151-1154, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28450956

RESUMO

Primary hypothyroidism usually leads to retardation of linear growth and delay or even arrest of puberty in juvenile patients. In rare cases, pediatric patients with hypothyroidism may present with signs of VanWyk-Grumbach's syndrome (VWGS), which includes juvenile hypothyroidism, delayed bone age and pseudoprecocious puberty. The present study reported on a rare case of VWGS and other complications, including obesity, short stature, hepatomegaly and pituitary hyperplasia. In addition, a comprehensive literature review was performed to illustrate the treatment and outcome of VWGS in pediatric patients. The present study contributed to the current knowledge regarding the diagnosis and treatment of VWGS in pediatric patients.

13.
Mol Med Rep ; 14(3): 2518-26, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27485938

RESUMO

The aim of the present study was to investigate the effect of testosterone on glucolipid metabolism and vascular injury in male rats, and examine the underlying molecular mechanisms. A total of 40 male Sprague-Dawley rats were divided into a control group (n=10), high-fat-diet + castration group (n=10), high­fat­diet + castration + low dose testosterone group (n=10), and high-fat-diet + castration + high dose testosterone group (n=10). Hematoxylin and eosin staining was performed to evaluate the morphology of the thoracic aortic tissues. Immunohistochemical staining was used to detect biomarkers of the phosphoinositide 3­kinase (PI3K) signaling pathway. The mRNA and protein expression levels of PI3K, AKT, insulin receptor substrate­1 (IRS­1), glucose transporter type 4 (GLUT­4), nuclear factor (NF)­κB and tumor necrosis factor (TNF)­α in the aortas were determined using quantitative polymerase chain reaction and Western blot analyses, respectively. Apoptosis in the aortic tissues was detected using a TUNEL assay. Castration induced apoptosis in the animals fed a high­fat­diet, whereas low dose testosterone replacement ameliorated the apoptosis in the aorta. However, the levels of apoptosis was more severe following high­dose testosterone treatment. Low­dose testosterone induced upregulation in the levels of IRS­1, AKT, GLUT­4 protein, NF­κB, TNF­α and PI3K, compared with those in the animals fed a high­fat diet following castration. A high dose of testosterone resulted in a significant decrease in the levels of IRS­1, AKT, GLUT­4, NF­κB, TNF­α and PI3K. Compared with the rats in the high­fat diet + castration group, a low dose of testosterone induced upregulation in the mRNA levels of IRS­1, AKT and GLUT­4, and downregulation of the mRNA levels of NF­κB, TNF­α and PI3K. A high dose of testosterone resulted in a significant decrease in the levels of IRS­1, AKT and GLUT­4, and marked increases in the mRNA levels of NF­κB, TNF­α and PI3K, compared with the low dose group. Castration induced marked disorders of glucolipid metabolism and vascular injuries in the pubescent male rats. Low­dose testosterone treatment was found to ameliorate the vascular damage caused by castration via the PI3K/AKT signaling pathway.


Assuntos
Orquiectomia/efeitos adversos , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Maturidade Sexual/efeitos dos fármacos , Transdução de Sinais/efeitos dos fármacos , Testosterona/farmacologia , Doenças Vasculares/etiologia , Doenças Vasculares/metabolismo , Animais , Aorta Torácica/metabolismo , Aorta Torácica/patologia , Biomarcadores , Modelos Animais de Doenças , Masculino , NF-kappa B/metabolismo , Ratos , Doenças Vasculares/tratamento farmacológico
14.
Oncotarget ; 7(35): 56193-56199, 2016 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-27486880

RESUMO

BACKGROUND: The expression levels and clinical significances of Lysosomal-associated protein transmembrane-4ß-35 (LAPTM4B-35) protein are unknown in the non-small-cell lung cancer (NSCLC). This study aimed to explore the expression and prognostic value of LAPTM4B-35 in NSCLC patients. METHODS: The clinicopathological and survival data of 107 NSCLC patients who received radical surgery from 2007 and 2011 were reviewed. The LAPTM4B-35 expression of the paired tumors and adjacent normal specimens were detected, and the association between LAPTM4B-35 and clinical variables was explored. Kaplan-Meier analysis and Cox regression (Proportional hazard model) were performed to investigate the prognostic significance for NSCLC. RESULTS: LAPTM4B-35 was over expressed in NSCLC tissues. The elevated LAPTM4B-35 expression was associated with cancer recurrence (P = 0.031). The 5-year median OS and PFS were significantly worse in the LAPTM4B-35 overexpressed group. Multivariate Cox analysis showed that LAPTM4B-35 over-expression was an independent factor for OS and PFS in NSCLC(P = 0.018, P = 0.026, respectively). CONCLUSIONS: The overexpressed LAPTM4B-35 was an independent prognostic biomarker for NSCLC, which could predict cancer recurrence and poor over survival. And that may be applied as potential target for NSCLC treatment.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/genética , Regulação Neoplásica da Expressão Gênica , Neoplasias Pulmonares/genética , Proteínas de Membrana/metabolismo , Recidiva Local de Neoplasia/genética , Proteínas Oncogênicas/metabolismo , Idoso , Biomarcadores Tumorais/metabolismo , Carcinoma Pulmonar de Células não Pequenas/mortalidade , Carcinoma Pulmonar de Células não Pequenas/patologia , Carcinoma Pulmonar de Células não Pequenas/cirurgia , Intervalo Livre de Doença , Feminino , Seguimentos , Humanos , Estimativa de Kaplan-Meier , Neoplasias Pulmonares/mortalidade , Neoplasias Pulmonares/patologia , Neoplasias Pulmonares/cirurgia , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia/patologia , Estadiamento de Neoplasias , Prognóstico , Modelos de Riscos Proporcionais , Fatores de Risco
15.
Oncotarget ; 7(34): 55765-55770, 2016 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-27303917

RESUMO

BACKGROUNDS: High serum C-reactive protein (CRP) was found to be associated with poor prognosis in kinds of solid tumors, however, its role in the recurrent gastric cancer (RGC) is unknown. The present study aimed to explore the prognostic value of serum CRP in RGC patients. METHODS: A total 72 RGC patients who underwent radical surgery from January 2005 to May 2008 were enrolled. The clinical, pathological and survival information were collected. The serum CRP level was measured when the recurrence was confirmed, and the association between serum CRP and clinicopathological characters was analyzed. The prognostic value of serum CRP for RGC was investigated. RESULTS: The serum CRP was elevated in 39 patients (H-CRP), while 33 patients were within the normal range (N-CRP).The elevated CRP was associated with Lymph node metastasis (p = 0.003) and tumor size (p = 0.004). The median survival time after recurrence was significantly worse in the H-CRP group than N-CRP group (6.5 months vs. 11.5 months, p = 0.012). Multivariate analyses identified that elevated CRP level (HR=2.325, p < 0.001), time to recurrence (HR = 0.466, p=0.033), and the follow-up treatment (HR = 2.650, p=0.001) were independent prognostic factors. CONCLUSIONS: High serum CRP level was associated with aggressive pathological features, was an independent poor prognostic factors for RGC, which might be a potential prognostic marker for RGC patients.


Assuntos
Proteína C-Reativa/análise , Recidiva Local de Neoplasia/mortalidade , Neoplasias Gástricas/mortalidade , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia/sangue , Recidiva Local de Neoplasia/patologia , Prognóstico , Modelos de Riscos Proporcionais , Neoplasias Gástricas/sangue , Neoplasias Gástricas/patologia
16.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 32(4): 451-6, 2016 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-27053608

RESUMO

OBJECTIVE: To study the correlation of coronin-1 and mycolic acid (MA)-induced foam cell formation of macrophages and explore its possible mechanism. METHODS: According to the difference of coronin-1 expression level, the experiment included three types of cells: RAW264.7-Cor.Plus, RAW264.7 and RAW264.7-Cor.Minus. After the cells were treated with the polystyrene microspheres coated with 100 µg/mL MA for 24 hours, total proteins were extracted and the level of coronin-1 in each group was detected by Western blotting. With the microspheres coated with 0, 25, 50, 75 and 100 µg/mL MA as phagocytic particles, the cells were swallowed for 24, 48, 72 hours, 5 and 8 days before and after the treatment with 2 µmol/L cytochalasin D (ctyD), and the levels of total cholesterol (TC) and free cholesterol (FC) were tested by TC enzyme kit and FC enzyme kit, respectively; then the cholesterol ester (CE) and the CE/TC ratio were used to quantitatively evaluate the level of foam cell formation. The ctyD-treated cells (RAW264.7-ctyD, RAW264.7-ctyD-MA) and their control groups were stained with FITC-phalloidin, and then the percentage of F-actin rearrangement was calculated. RESULTS: After MA induction, the coronin-1 level of the three experimental groups were significantly higher than that of the corresponding control groups, and there was also obvious difference between the three experimental groups (RAW264.7-Cor.Plus>RAW264.7>RAW264.7-Cor.Minus). The level of foam cell formation of macrophages in each group with different coronin-1 level was positively correlated with the MA coating concentration and its phagocytic time. The highest coronin-1 expression group (RAW264.7-Cor.Plus) had the highest foam cell formation level, and the lowest coronin-1 expression group (RAW264.7-Cor.Minus) had the lowest foam cell formation level. The inhibition of F-actin by ctyD significantly decreased the foam cell formation induced by MA, but the inhibition of F-actin had no significant impact on the positive correlation between coronin-1 and the foam cell formation induced by MA. After phalloidin staining, the F-actin rearrangement rate of MA-treated cells was significantly higher than that of non-MA control cells. CONCLUSION: MA could induce the expression of coronin-1 of macrophage, and the coronin-1 level was positively correlated with the foam cell formation induced by MA. F-actin was involved in the process of lipid accumulation in MA-treated macrophages, but the F-actin was not the key or the only way in coronin-1 regulating the foam cell formation induced by MA.


Assuntos
Células Espumosas/efeitos dos fármacos , Macrófagos/efeitos dos fármacos , Proteínas dos Microfilamentos/metabolismo , Ácidos Micólicos/farmacologia , Animais , Diferenciação Celular/efeitos dos fármacos , Células Espumosas/citologia , Células Espumosas/metabolismo , Macrófagos/citologia , Macrófagos/metabolismo , Camundongos , Proteínas dos Microfilamentos/genética , Células RAW 264.7
17.
J Pediatr Endocrinol Metab ; 29(12): 1331-1335, 2016 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-26959535

RESUMO

BACKGROUND: The diagnosis of non-alcoholic fatty liver disease (NAFLD) is usually based on liver ultrasonography and serum alanine aminotransferase (ALT) levels. However, the serum ALT level is not sensitive for detecting NAFLD. If more serum markers are available, serum analysis may play a more important role in the diagnosis of NAFLD. METHODS: Here, we have investigated whether vascular endothelial cadherin (VE-cad) and thrombomodulin (TM) are markers of NAFLD in children. After an examination of liver ultrasonography, 90 children were divided into a lean control group (n=32), an overweight/obese NAFLD group (group-NAFLD, n=34) and an overweight/obese non-NAFLD group (group-SOO, n=24). RESULTS: Two overweight/obese groups had similar obesity. However, serum VE-cad and TM levels were increased in group-NAFLD but not group-SOO. When data from all children were pooled, serum VE-cad and TM levels were positively correlated to body-mass index (BMI) and serum ALT levels. CONCLUSIONS: In conclusion, VE-cad and TM are markers of pediatric NAFLD.


Assuntos
Antígenos CD/sangue , Biomarcadores/sangue , Caderinas/sangue , Hepatopatia Gordurosa não Alcoólica/diagnóstico , Obesidade/complicações , Sobrepeso/complicações , Trombomodulina/sangue , Adolescente , Estudos de Casos e Controles , Criança , Feminino , Humanos , Resistência à Insulina , Masculino , Hepatopatia Gordurosa não Alcoólica/sangue , Hepatopatia Gordurosa não Alcoólica/etiologia , Prognóstico
18.
Int J Clin Exp Med ; 8(8): 13491-9, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26550286

RESUMO

OBJECTIVE: Metabolic syndrome (MS) is conceived as the pathogenic basis of an increased cardiovascular burden. We investigate the correlation between interleukin-6 (IL-6) and the risk factors of MS and cardiovascular disease (CVD) in diet-induced model of MS and determined whether IL-6 was associated with the prevalence of MS and cardiovascular disease. METHODS: A total of 40 Spague-Dawley (SD) rats were randomly divided into high-fat and high salt (FSC) group, high-fat (FC) group and normal control (NC) group. After feeding for 7 weeks, fasting blood glucose (FBG) and fasting insulin (FIN) were measured at the 60 min, 120 min and 180 min after the glucose administration. Blood pressure, body weight, height, waist circumference (WC), liver weight, visceral fat weight as well as blood lipid profile were determined at the end of 7-week. Furthermore, IL-6 levels from adipose tissues were analyzed using ELISA, and the correlation between IL-6 and the risk factors of MS and cardiovascular disease was investigated. RESULTS: After treatment with different diets, significant difference was noted in the WC, body mass index (BMI), visceral fat weight and liver weight of FSC group compared with those of NC group (P<0.05). The levels of systolic blood pressure (SBP), diastolic blood pressure (DBP), total cholesterol (TC), triacylglycerol (TG) and low density lipoprotein (LDL) were markedly elevated in FSC group compared with those in NC group, while the level of high density lipoprotein (HDL) was remarkable lower in FSC group (P<0.05). After glucose administration, the concentrations of blood glucose and insulin were significant higher in FSC group than those in NC group at different time points (P<0.05). Moreover, high-fat and high salt diet brought about significant elevation of IL-6 compared with that with normal or high-fat diet in SD rats. Furthermore, IL-6 was significantly associated with FIN, HOMA-IR, LDL, TC, TG, HDL, visceral fat mass and body weight in FSC group, while IL-6 was markedly correlated with TC, LDL, TG, visceral fat mass and body weight (P<0.05). CONCLUSION: A characteristic rat model of MS may be induced by the high-fat and high-salt diet. IL-6 may be considered as an early and representative marker in the pathogenesis of MS and related cardiovascular burden.

19.
Int J Clin Exp Pathol ; 8(8): 9468-70, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26464706

RESUMO

Coronary artery aneurysm or ectasia was reported in approximately 15% to 25% of the affected children, particularly in the proximal end of the main blood vessel and the left anterior descending part. Rare patients have been reported with aneurysm in the distal end of the right coronary artery. In this case report, we present a rare case with aneurysm in the distal end of the right coronary artery. Multi-slice computed tomography was performed for the coronary angiography. Aspirin (10 mg/kg body weight per day) and gamma globulin (2 kg/kg body weight) was administrated via intravenous injection. The patient is currently in a healthy status with a 12-month follow up.


Assuntos
Aneurisma Coronário/etiologia , Síndrome de Linfonodos Mucocutâneos/complicações , Aspirina/uso terapêutico , Criança , Aneurisma Coronário/diagnóstico por imagem , Aneurisma Coronário/tratamento farmacológico , Angiografia Coronária , Fibrinolíticos/uso terapêutico , Humanos , Masculino , Síndrome de Linfonodos Mucocutâneos/diagnóstico por imagem , Resultado do Tratamento , gama-Globulinas/uso terapêutico
20.
Exp Ther Med ; 9(6): 2379-2383, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26136991

RESUMO

The aim of the present study was to outline any predisposing factors and clinical and radiological features of post-infectious bronchiolitis obliterans (PIBO) in pediatric patients, and to determine the effect of long-term azithromycin treatment on these factors. In total, 16 cases of children with PIBO were retrospectively reviewed. Adenovirus and Mycoplasma pneumoniae were the most common etiological agents (12/16) in the children with PIBO. The patients presented with persistent dyspnea, a chronic cough, sputum production and wheezing following the initial lung infection. Chest X-rays indicated pulmonary overinflation and patchy ground-glass opacity. In addition, high-resolution computed tomography (HRCT) scans revealed patchy ground-glass opacity, bronchiectasis, bronchial wall thickening and mosaic perfusion in all 16 cases. A unilateral hyperlucent lung was observed in two cases. All the patients underwent treatment with low-dose azithromycin and prednisone. Follow-up examinations of the 16 cases, varying in duration between 7 and 31 months, showed that the disease condition had improved in 10 cases. However, no significant improvements were identified from the HRCT scans or were observed in the patient condition in the additional six cases. The diagnosis of BO is primarily based on a typical clinical presentation and HRCT observations. Therefore, a typical clinical history and patchy ground-glass opacity features on HRCT scans are screening indices that predict BO development. Steroids are the cornerstone of BO treatment; however, long-term azithromycin treatment can improve the condition of the patients. In summary, PIBO is a disease with a high morbidity rate and should be treated by a multidisciplinary team. Patients should receive follow-up examination for an extended period. Patchy ground-glass opacity features on HRCT scans indicate that clinical suspicion of BO is necessary in children with persistent and severe wheezing.

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