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1.
Exp Mol Pathol ; : 104304, 2019 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-31479659

RESUMO

Diabetes induces time-dependent alterations in urinary bladders. Long-term diabetes causes an underactive bladder. However, the fundamental mechanisms are still elusive. This study aimed to examine the histological changes and the potential molecular pathways affected by long-term diabetes in the rat bladder. Diabetes was induced in 8-week-old male Lewis rats by streptozotocin, while age-matched control rats received citrate buffer only. Forty-four weeks after diabetes induction, bladders were harvested for histological and molecular analyses. The expressions of proteins related to fibrosis, apoptosis and oxidative stress as well as the cellular signaling pathway in the bladder were examined by immunoblotting. Histological examinations illustrated diabetes caused detrusor hypertrophy and fibrotic changes in the bladder. Immunoblotting analysis demonstrated higher collagen I but lower elastin expression in the bladder in diabetic rats. These were accompanied by an increase in the expression of transforming growth factor-beta1, along with the downregulation of matrix metalloptoteinase-1, and upregulation of tissue inhibitor of metalloproteinase-1. Diabetic rats showed an increase in nitrotyrosine, but decrease in nuclear factor erythroid-related factor 2 (Nrf2) levels in the bladder. Enhanced apoptotic signaling was observed, characterized by increased expression of Bcl-2-associated X protein (Bax), decreased expression of Bcl-2, in the diabetic bladder. The nerve growth factor level was decreased in the diabetic bladder. A significant suppression in the protein expressions of phosphorylated extracellular signal-regulated kinases 1/2 was found in diabetic bladders. This study demonstrated that long-term diabetes caused molecular changes that could promote fibrosis and apoptosis in the bladder. Oxidative stress may be involved in this context.

2.
Biomed Chromatogr ; : e4680, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31415097

RESUMO

In this study, an accurate and reliable method of ultra-performance liquid chromatography coupled with a triple-quadrupole tandem mass spectrometry was firstly developed and fully validated for the simultaneous determination of epicatechin, neoastilbin, astilbin, isoastilbin, engeletin and resveratrol in rat plasma after administration of Smilacis glabrae Roxb. extract. Naringenin was used as an internal standard (IS). The analyte and IS were separated on a C18 column by gradient elution with a mobile phase of acetonitrile-0.3% acetic acid at a flow rate of 0.25 mL/min for a total run time of 8 min. The method was validated in terms of selectivity, linearity, precision, accuracy, extraction recovery, matrix effect and stability. The developed method was successfully applied to determine the main pharmacokinetic parameters of six components in rat plasma.

3.
Am J Physiol Renal Physiol ; 317(4): F906-F912, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31241994

RESUMO

Bladder dysfunction in diabetes progresses gradually over time. However, the mechanisms of the development are not clear. We tested the hypothesis that oxidative stress plays a key role in the development of diabetic bladder dysfunction using an inducible smooth muscle (SM)-specific superoxide dismutase 2 (Sod2) gene knockout (SM-Sod2 KO) mouse model. Eight-week-old male Sod2lox/lox, SM-CreERT2(ki)Cre/+ mice and wild-type mice were assigned to diabetic or control groups. 4-Hydroxytamoxifen was injected into Sod2lox/lox, SM-CreERT2(ki)Cre/+ mice to activate CreERT2-mediated deletion of Sod2. Diabetes was induced by injection of streptozotocin, whereas control mice were injected with vehicle. Nine weeks later, bladder function was evaluated, and bladders were harvested for immunoblot analysis. Wild-type diabetic mice presented compensated bladder function along with increased nitrotyrosine and MnSOD in detrusor muscle. Induction of diabetes in SM-Sod2 KO mice caused deteriorated bladder function and even greater increases in nitrotyrosine compared with wild-type diabetic mice. Expression levels of apoptosis regulator Bax and cleaved caspase-3 were increased, but apoptosis regulator Bcl-2 expression was decreased in detrusor muscle of both diabetic groups, with more pronounced effects in SM-Sod2 KO diabetic mice. Our findings demonstrate that exaggerated oxidative stress can accelerate the development of bladder dysfunction in diabetic mice and the enhanced activation of apoptotic pathways in the bladder may be involved in the process.

4.
Nat Commun ; 10(1): 1494, 2019 04 02.
Artigo em Inglês | MEDLINE | ID: mdl-30940818

RESUMO

A complete and accurate genome sequence provides a fundamental tool for functional genomics and DNA-informed breeding. Here, we assemble a high-quality genome (contig N50 of 6.99 Mb) of the apple anther-derived homozygous line HFTH1, including 22 telomere sequences, using a combination of PacBio single-molecule real-time (SMRT) sequencing, chromosome conformation capture (Hi-C) sequencing, and optical mapping. In comparison to the Golden Delicious reference genome, we identify 18,047 deletions, 12,101 insertions and 14 large inversions. We reveal that these extensive genomic variations are largely attributable to activity of transposable elements. Interestingly, we find that a long terminal repeat (LTR) retrotransposon insertion upstream of MdMYB1, a core transcriptional activator of anthocyanin biosynthesis, is associated with red-skinned phenotype. This finding provides insights into the molecular mechanisms underlying red fruit coloration, and highlights the utility of this high-quality genome assembly in deciphering agriculturally important trait in apple.


Assuntos
Genoma de Planta , Malus/genética , Retroelementos , Cor , Frutas/química , Frutas/genética , Genômica , Malus/química , Malus/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Sequências Repetidas Terminais , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
5.
ACS Appl Mater Interfaces ; 11(2): 2140-2148, 2019 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-30569697

RESUMO

Although stretchable transparent conductors, stemmed from the strategies of both conductive composite and structural design of nonstretchable conductors, have been extensively studied, these conductors either suffer from low stretchability or require a complex fabrication process, which drastically limits their practical applications. Here, we propose a novel strategy combining the design of substrates and a simple template-assisted transfer printing process to fabricate three-dimensional (3D) transparent conductors. The strategy not only eliminates the complex and costly fabrication processes but it also endows conductors with high stretchability and long-term stability, thanks to the controllable strain distribution as well as the seamless connection between the conductor layer and the substrate. These newly designed 3D conductors achieve a low sheet resistance of 1.0 Ω/sq with a high transmittance of above 85% and remain stable without obvious resistance change during 1000 stretching-relaxation cycles until 60% strain, which are superior to most reported conductors. A large-area stretchable heater based on the 3D conductor realizes the temperature fluctuation below 10% even under a large strain, thus showing huge application prospects in the field of wearable healthcare electronics. The simple solution-processed fabrication method and high performance such as stretchability and low resistance change over a large strain range promote the practical applications of these newly designed 3D conductors.

6.
Clin Exp Hypertens ; : 1-7, 2018 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-29667441

RESUMO

Western diet (WD), rich in saturated fat and sugars, has become a risk factor for obesity and metabolic syndrome, however, its effect on endothelial function and vascular remodeling is not fully elucidated. Recent evidence suggests cross-talk between Rho kinase (ROCK) pathway and cardiovascular system. We aimed to investigate the effect of WD on aortic remodeling and the contribution of ROCK signaling. Eight week old male Sprague-Dawley rats were fed either standard chow diet (SD) or high fructose/ high-fat diet, typically as in WD. After 42 weeks, WD-fed rats showed hyperglycemia, dyslipidemia, and hypertension without marked weight gain, compared to SD-fed rats. Significant up-regulation of ROCK-1 and -2, along with a decline in eNOS expression were found in the aortic tissue of WD-fed rats. Additionally, WD-fed rats displayed oxidative stress and fibrosis in their aortic tissues versus controls. Our findings suggest that long-term feeding of WD contributes to endothelial dysfunction and aortic remodeling in adult male rats. ROCK activation seems to be involved in WD-related vascular disorders and may represent a promising therapeutic target.

7.
Biomed Res Int ; 2018: 2925985, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29511675

RESUMO

Enhanced spontaneous contractions are associated with overactive bladder. Elevated levels of reactive oxygen species might contribute to enhanced spontaneous contractions. We investigated the regulation of spontaneous contractions and the effects of hydrogen peroxide (H2O2) in intact rat bladder strips. The spontaneous contractions were measured using a tissue bath system. The vehicle or the specific activators/blockers were applied and followed by the application of 0.003 g% H2O2. The basal tension, amplitude, and frequency of spontaneous contractions were quantified. Nisoldipine and bisindolylmaleimide 1 had no effects on spontaneous contractions. SKF96365 and Y27632 decreased basal tension and amplitude. Ryanodine slightly increased frequency. Both iberiotoxin and NS-1619 increased amplitude. Apamin reduced frequency but increased amplitude. NS-309 inhibited both the amplitude and frequency. The basal tension and amplitude increased when H2O2 was applied. Pretreatment with NS-309 inhibited H2O2-elicited augmented amplitude and frequency, while pretreatment with Y-27632 inhibited the augmented basal tension. The combined application of NS-309 and Y27632 almost eliminated spontaneous contractions and its augmentation induced by H2O2. In conclusion, Ca2+ influx, Rho kinase activation, and SK channel inactivation play important roles in spontaneous contractions in intact bladder strips, whereas only latter two mechanisms may be involved in H2O2-elicited increased spontaneous contractions.


Assuntos
Peróxido de Hidrogênio/administração & dosagem , Contração Muscular/efeitos dos fármacos , Bexiga Urinária Hiperativa/tratamento farmacológico , Bexiga Urinária/efeitos dos fármacos , Amidas/administração & dosagem , Animais , Imidazóis/administração & dosagem , Indóis/administração & dosagem , Masculino , Maleimidas/administração & dosagem , Músculo Liso/efeitos dos fármacos , Nisoldipino/administração & dosagem , Oximas/administração & dosagem , Piridinas/administração & dosagem , Ratos , Rianodina/administração & dosagem , Canais de Potássio Ativados por Cálcio de Condutância Baixa/genética , Bexiga Urinária/fisiologia , Bexiga Urinária Hiperativa/fisiopatologia , Quinases Associadas a rho/genética
8.
Genomics ; 110(3): 162-170, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-28935392

RESUMO

Paecilomyces hepialid (PH) is an endoparasitic fungus of Cordyceps sinensis (CS) and has become a substitute for CS due to their similar pharmacological activities. Because the market demand for CS is difficult to satisfy, and cordycepin, the effective compound of CS, is difficult to industrially produce, we produced 5 samples of PH by culturing for different durations and adding different additives to the culture broth, and detected their cordycepin content with UPLC ESI MS/MS. Then we grouped these cultures into five transcriptome comparisons containing 3 time variable groups and 2 additive variable groups. We used next-generation (NG) sequencing methods to acquire transcriptomic information and investigated the response of gene expression to the additives and the influence of different growth stages. This work will contribute to a better understanding of purine metabolism in PH, and possibly in other Cordyceps species. It will provide a useful resource to further advance transcriptomics studies in Cordyceps species.


Assuntos
Vias Biossintéticas , Desoxiadenosinas/biossíntese , Paecilomyces/metabolismo , Perfilação da Expressão Gênica , Paecilomyces/genética , Paecilomyces/crescimento & desenvolvimento , Espectrometria de Massas em Tandem
9.
Int. braz. j. urol ; 43(4): 753-761, July-Aug. 2017. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-892855

RESUMO

ABSTRACT Objective Diabetes affects the erectile function significantly. However, the penile alterations in the early stage of diabetes in experimental animal models have not been well studied. We examined the changes of the penis and its main erectile components in diabetic rats. Materials and methods Male Sprague-Dawley rats were divided into 2 groups: streptozotocin (STZ)-induced diabetics and age-matched controls. Three or nine weeks after diabetes induction, the penis was removed for immunohistochemical staining of smooth muscle and neuronal nitric oxide synthase (nNOS) in midshaft penile tissues. The cross-sectional areas of the whole midshaft penis and the corpora cavernosa were quantified. The smooth muscle in the corpora cavernosa and nNOS in the dorsal nerves were quantified. Results The weight, but not the length, of the penis was lower in diabetics. The cross-sectional areas of the total midshaft penis and the corpora cavernosa were lower in diabetic rats compared with controls 9 weeks, but not 3 weeks after diabetes induction. The cross-sectional area of smooth muscle in the corpora cavernosa as percentage of the overall area of the corpora cavernosa was lower in diabetic rats than in controls 9 weeks, but not 3 weeks after diabetes induction. Percentage change of nNOS in dorsal nerves was similar at 3 weeks, and has a decreased trend at 9 weeks in diabetic rats compared with controls. Conclusions Diabetes causes temporal alterations in the penis, and the significant changes in STZ rat model begin 3-9 weeks after induction. Further studies on the reversibility of the observed changes are warranted.

10.
Ann Clin Microbiol Antimicrob ; 16(1): 45, 2017 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-28646898

RESUMO

BACKGROUND: The purpose of this study was to evaluate the prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium and Ureaplasma urealyticum infections in infertile men that consulted our outpatient departments using a novel simultaneous amplification testing (SAT) that is RNA-detection based. The possible impact of C. trachomatis, N. gonorrhoeae, M. genitalium and U. urealyticum infections on semen parameters was also noted in the present study. METHODS: A total of 2607 males that were diagnosed with infertility were included in this study. C. trachomatis, N. gonorrhoeae, M. genitalium and U. urealyticum infections were detected in the urine samples using SAT method. Related data, including semen parameters and age as well as C. trachomatis, N. gonorrhoeae, M. genitalium and U. urealyticum infections were collected and analyzed. RESULTS: A total of 51 and 1418 urine samples were found positive for M. genitalium RNA and U. urealyticum RNA, respectively, while the prevalence of C. trachomatis and N. gonorrhoeae was relatively lower. Men with positive M. genitalium RNA and U. urealyticum RNA had higher sperm DNA fragmentation index (DFI) while the comparisons of other semen parameters yielded nonsignificant results between the RNA positive and negative group. A multivariate linear regression analysis revealed that U. urealyticum and M. genitalium infections posed significant factors of DFI (adjusted R2 = 46.2%). CONCLUSIONS: Our study suggested a relative high prevalence of U. urealyticum and M. genitalium infection based on this novel SAT detection method. U. urealyticum and M. genitalium infection could possibly impair male fertility potential through promoting sperm DNA damage.


Assuntos
Chlamydia trachomatis/isolamento & purificação , Infertilidade Masculina/microbiologia , Mycoplasma genitalium/isolamento & purificação , Neisseria gonorrhoeae/isolamento & purificação , Técnicas de Amplificação de Ácido Nucleico/métodos , Ureaplasma urealyticum/isolamento & purificação , Adulto , China/epidemiologia , Chlamydia trachomatis/genética , Humanos , Infertilidade Masculina/epidemiologia , Masculino , Mycoplasma genitalium/genética , Neisseria gonorrhoeae/genética , Prevalência , RNA Bacteriano/genética , Ureaplasma urealyticum/genética , Urina/microbiologia , Adulto Jovem
11.
Gigascience ; 6(4): 1-5, 2017 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-28327946

RESUMO

The Northern snakehead (Channa argus), a member of the Channidae family of the Perciformes, is an economically important freshwater fish native to East Asia. In North America, it has become notorious as an intentionally released invasive species. Its ability to breathe air with gills and migrate short distances over land makes it a good model for bimodal breath research. Therefore, recent research has focused on the identification of relevant candidate genes. Here, we performed whole genome sequencing of C. argus to construct its draft genome, aiming to offer useful information for further functional studies and identification of target genes related to its unusual facultative air breathing. Findings: We assembled the C. argus genome with a total of 140.3 Gb of raw reads, which were sequenced using the Illumina HiSeq2000 platform. The final draft genome assembly was approximately 615.3 Mb, with a contig N50 of 81.4 kb and scaffold N50 of 4.5 Mb. The identified repeat sequences account for 18.9% of the whole genome. The 19 877 protein-coding genes were predicted from the genome assembly, with an average of 10.5 exons per gene. Conclusion: We generated a high-quality draft genome of C. argus, which will provide a valuable genetic resource for further biomedical investigations of this economically important teleost fish.


Assuntos
Genoma , Genômica , Perciformes/genética , Animais , Biologia Computacional/métodos , Tamanho do Genoma , Genômica/métodos , Anotação de Sequência Molecular , Família Multigênica , Perciformes/classificação , Filogenia , Sequências Repetitivas de Ácido Nucleico , Análise de Sequência de DNA , Sequenciamento Completo do Genoma
12.
Int Braz J Urol ; 43(4): 753-761, 2017 Jul-Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28338308

RESUMO

OBJECTIVE: Diabetes affects the erectile function significantly. However, the penile alterations in the early stage of diabetes in experimental animal models have not been well studied. We examined the changes of the penis and its main erectile components in diabetic rats. MATERIALS AND METHODS: Male Sprague-Dawley rats were divided into 2 groups: streptozotocin (STZ)-induced diabetics and age-matched controls. Three or nine weeks after diabetes induction, the penis was removed for immunohistochemical staining of smooth muscle and neuronal nitric oxide synthase (nNOS) in midshaft penile tissues. The cross-sectional areas of the whole midshaft penis and the corpora cavernosa were quantified. The smooth muscle in the corpora cavernosa and nNOS in the dorsal nerves were quantified. RESULTS: The weight, but not the length, of the penis was lower in diabetics. The crosssectional areas of the total midshaft penis and the corpora cavernosa were lower in diabetic rats compared with controls 9 weeks, but not 3 weeks after diabetes induction. The cross-sectional area of smooth muscle in the corpora cavernosa as percentage of the overall area of the corpora cavernosa was lower in diabetic rats than in controls 9 weeks, but not 3 weeks after diabetes induction. Percentage change of nNOS in dorsal nerves was similar at 3 weeks, and has a decreased trend at 9 weeks in diabetic rats compared with controls. CONCLUSIONS: Diabetes causes temporal alterations in the penis, and the significant changes in STZ rat model begin 3-9 weeks after induction. Further studies on the reversibility of the observed changes are warranted.


Assuntos
Diabetes Mellitus Experimental/complicações , Diabetes Mellitus Tipo 1/complicações , Disfunção Erétil/etiologia , Músculo Liso/fisiopatologia , Pênis/fisiopatologia , Animais , Diabetes Mellitus Experimental/fisiopatologia , Diabetes Mellitus Tipo 1/fisiopatologia , Disfunção Erétil/fisiopatologia , Masculino , Óxido Nítrico Sintase Tipo I/metabolismo , Pênis/inervação , Ratos , Ratos Sprague-Dawley
13.
Am J Physiol Renal Physiol ; 312(5): F887-F896, 2017 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-28052873

RESUMO

Diabetes mellitus (DM) is a prevalent chronic disease. Type 1 DM (T1DM) is a metabolic disorder that is characterized by hyperglycemia in the context of absolute lack of insulin, whereas type 2 DM (T2DM) is due to insulin resistance-related relative insulin deficiency. In comparison with T1DM, T2DM is more complex. The natural history of T2DM in most patients typically involves a course of obesity to impaired glucose tolerance, to insulin resistance, to hyperinsulinemia, to hyperglycemia, and finally to insulin deficiency. Obesity is a risk factor of T2DM. Diabetes causes some serious microvascular and macrovascular complications, such as retinopathy, nephropathy, neuropathy, angiopathy and stroke. Urological complications of obesity and diabetes (UCOD) affect quality of life, but are not well investigated. The urological complications in T1DM and T2DM are different. In addition, obesity itself affects the lower urinary tract. The aim of this perspective is to review the available data, combined with the experience of our research teams, who have spent a good part of last decade on studies of association between DM and lower urinary tract symptoms (LUTS) with the aim of bringing more focus to the future scientific exploration of UCOD. We focus on the most commonly seen urological complications, urinary incontinence, bladder dysfunction, and LUTS, in obesity and diabetes. Knowledge of these associations will lead to a better understanding of the pathophysiology underlying UCOD and hopefully assist urologists in the clinical management of obese or diabetic patients with LUTS.


Assuntos
Diabetes Mellitus Tipo 1/complicações , Diabetes Mellitus Tipo 2/complicações , Sintomas do Trato Urinário Inferior/etiologia , Obesidade/complicações , Próstata/fisiopatologia , Pesquisa Médica Translacional , Doenças da Bexiga Urinária/etiologia , Bexiga Urinária/fisiopatologia , Incontinência Urinária/etiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Diabetes Mellitus Tipo 1/metabolismo , Diabetes Mellitus Tipo 1/fisiopatologia , Diabetes Mellitus Tipo 2/metabolismo , Diabetes Mellitus Tipo 2/fisiopatologia , Feminino , Humanos , Sintomas do Trato Urinário Inferior/metabolismo , Sintomas do Trato Urinário Inferior/fisiopatologia , Masculino , Pessoa de Meia-Idade , Obesidade/metabolismo , Obesidade/fisiopatologia , Prognóstico , Próstata/metabolismo , Fatores de Risco , Bexiga Urinária/metabolismo , Doenças da Bexiga Urinária/metabolismo , Doenças da Bexiga Urinária/fisiopatologia , Incontinência Urinária/metabolismo , Incontinência Urinária/fisiopatologia , Adulto Jovem
14.
Mol Biol Evol ; 34(1): 145-159, 2017 01.
Artigo em Inglês | MEDLINE | ID: mdl-28007977

RESUMO

The Amur ide (Leuciscus waleckii) is a cyprinid fish that is widely distributed in Northeast Asia. The Lake Dali Nur population inhabits one of the most extreme aquatic environments on Earth, with an alkalinity up to 50 mmol/L (pH 9.6), thus providing an exceptional model with which to characterize the mechanisms of genomic evolution underlying adaptation to extreme environments. Here, we developed the reference genome assembly for L. waleckii from Lake Dali Nur. Intriguingly, we identified unusual expanded long terminal repeats (LTRs) with higher nucleotide substitution rates than in many other teleosts, suggesting their more recent insertion into the L. waleckii genome. We also identified expansions in genes encoding egg coat proteins and natriuretic peptide receptors, possibly underlying the adaptation to extreme environmental stress. We further sequenced the genomes of 10 additional individuals from freshwater and 18 from Lake Dali Nur populations, and we detected a total of 7.6 million SNPs from both populations. In a genome scan and comparison of these two populations, we identified a set of genomic regions under selective sweeps that harbor genes involved in ion homoeostasis, acid-base regulation, unfolded protein response, reactive oxygen species elimination, and urea excretion. Our findings provide comprehensive insight into the genomic mechanisms of teleost fish that underlie their adaptation to extreme alkaline environments.


Assuntos
Adaptação Fisiológica/genética , Evolução Biológica , Cyprinidae/genética , Animais , Ásia , Evolução Molecular , Ambientes Extremos , Feminino , Perfilação da Expressão Gênica/métodos , Estudos de Associação Genética , Genômica/métodos , Concentração de Íons de Hidrogênio , Lagos , Análise de Sequência de DNA/métodos , Estresse Fisiológico/genética , Transcriptoma
15.
Genome Announc ; 4(4)2016 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-27389266

RESUMO

Paecilomyces hepiali is an endoparasitic fungus that commonly exists in the natural Cordyceps sinensis Here, we report the draft genome sequence of P. hepiali, which will facilitate the exploitation of medicinal compounds produced by the fungus.

16.
J Diabetes Complications ; 30(5): 778-85, 2016 07.
Artigo em Inglês | MEDLINE | ID: mdl-27037041

RESUMO

AIMS: Diabetic bladder dysfunction (DBD) has been extensively studied in animal models of type 1 diabetes. We aimed to examine the functional and morphological alterations of the urinary bladder in a type 2 diabetes model, FVB(db/db) mice. METHODS: FVB(db/db) mice and age-matched FVB/NJ control mice were tested at either 12, 24 or 52weeks of age. Body weight, blood glucose and glycated hemoglobin (HbA1c) levels were measured. Bladder function was assessed by measurement of 24-h urination behavior and conscious cystometry. Bladder was harvested for Masson's Trichrome staining and morphometric analysis. RESULTS: The body weights of FVB(db/db) mice were twice as those of FVB/NJ control mice. The blood glucose and HbA1c levels were higher in FVB(db/db) mice at 12 and 24weeks, but not at 52weeks. A significant increase in the mean volume per void, but decrease in the voiding frequency, in FVB(db/db) mice was observed. Cystometry evaluation showed increased bladder capacity, voided volume, and peak micturition pressure in FVB(db/db) mice compared with FVB/NJ mice. Morphometric analysis revealed a significant increase in the areas of detrusor muscle and urothelium in FVB(db/db) mice. In addition, some FVB(db/db) mice, especially males at 12 and 24weeks, showed small-volume voiding during 24-h urination behavior measurement, and detrusor overactivity in the cystometry measurement. CONCLUSIONS: The FVB(db/db) mouse, displaying DBD characterized by not only increased bladder capacity, void volume, and micturition pressure, but also bladder overactivity, is a useful model to further investigate the mechanisms of type 2 diabetes-related bladder dysfunction.


Assuntos
Envelhecimento , Complicações do Diabetes/fisiopatologia , Diabetes Mellitus Tipo 2/complicações , Obesidade/complicações , Bexiga Urinária Hiperativa/complicações , Bexiga Urinária/fisiopatologia , Urotélio/fisiopatologia , Animais , Comportamento Animal , Glicemia , Complicações do Diabetes/sangue , Complicações do Diabetes/patologia , Ingestão de Líquidos , Feminino , Hemoglobina A Glicada/análise , Masculino , Camundongos Endogâmicos , Camundongos Mutantes , Tamanho do Órgão , Caracteres Sexuais , Bexiga Urinária/patologia , Bexiga Urinária Hiperativa/sangue , Bexiga Urinária Hiperativa/patologia , Bexiga Urinária Hiperativa/fisiopatologia , Micção , Urotélio/patologia , Ganho de Peso
17.
Prostate ; 76(11): 964-76, 2016 08.
Artigo em Inglês | MEDLINE | ID: mdl-27040645

RESUMO

OBJECTIVE: Accumulating evidences suggests that obesity and metabolic syndrome (MetS) contribute towards lower urinary tract symptoms (LUTS) through alterations in the phenotype of bladder and prostate gland. Clinical studies indicate a link between MetS and LUTS. Nevertheless, there is lack of suitable animal model(s) which could illustrate an association linking obesity to LUTS. We examined the lower urinary tract function in an obesity-initiated MetS mouse model. METHODS: Male C57BL/6N wild-type and obese B6.V-Lepob/J maintained on regular diet for 28 weeks were subjected to the assessment of body weight (BW), body length (BL), waist circumference (WC), body mass index (BMI), blood glucose (BG), plasma insulin (INS), plasma leptin (LEP), total cholesterol (CHO), free fatty acid (FFA), and measurement of urinary functions. Whole animal peritoneal and subcutaneous adipose tissue measurements as well as prostate and bladder volumes were analyzed by MRI followed by histological evaluation. These parameters were used to draw correlations between MetS and LUTS. RESULTS: Obesity parameters such as BW, WC, and BMI were significantly higher in B6.V-Lepob/J mice compared to C57BL/6N mice (P < 0.01). Higher levels of total CHO and FFA were noted in B6.V-Lepob/J mice than C57BL/6N mice (P < 0.05). These results were concurrent with frequency, lower average urine volume and other urinary voiding dysfunctions in B6.V-Lepob/J mice. MRI assessments demonstrate marked increase in body fat and prostate volume in these mice. Compared to C57BL/6N mice, histological analysis of the prostate from B6.V-Lepob/J mice showed increased proliferation, gland crowding, and infiltration of immune cells in the stroma; whereas the bladder urothelium was slightly thicker and appears more proliferative in these mice. The regression and correlation analysis indicate that peritoneal fat (R = 0.853; P < 0.02), CHO (R = 0.729; P < 0.001), BG (R = 0.712; P < 0.001) and prostate volume (R = 0.706; P < 0.023) strongly correlate with LUTS whereas BMI, WC, INS, and FFA moderately correlate with the prevalence of bladder dysfunction. CONCLUSION: Our results suggest that LUTS may be attributable in part to obesity and MetS. Validation of an in vivo model may lead to understand the underlying pathophysiological mechanisms of obesity-related LUTS in humans. Prostate 76:964-976, 2016. © 2016 Wiley Periodicals, Inc.


Assuntos
Síndrome Metabólica/complicações , Obesidade/complicações , Transtornos Urinários/etiologia , Tecido Adiposo/patologia , Animais , Biometria , Composição Corporal , Colesterol/sangue , Modelos Animais de Doenças , Leptina/sangue , Sintomas do Trato Urinário Inferior/etiologia , Sintomas do Trato Urinário Inferior/patologia , Sintomas do Trato Urinário Inferior/fisiopatologia , Imagem por Ressonância Magnética , Masculino , Síndrome Metabólica/fisiopatologia , Camundongos , Camundongos Endogâmicos C57BL , Obesidade/fisiopatologia , Peritônio , Próstata/patologia , Bexiga Urinária/patologia , Transtornos Urinários/patologia , Transtornos Urinários/fisiopatologia
18.
Sci Rep ; 6: 24015, 2016 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-27052994

RESUMO

Although polyhydroxyalkanoate (PHA) accumulation and mobilization are one of the most general mechanisms for haloarchaea to adapt to the hypersaline environments with changeable carbon sources, the PHA mobilization pathways are still not clear for any haloarchaea. In this study, the functions of five putative (R)-specific enoyl-CoA hydratases (R-ECHs) in Haloferax mediterranei, named PhaJ1 to PhaJ5, respectively, were thoroughly investigated. Through gene deletion and complementation, we demonstrated that only certain of these ECHs had a slight contribution to poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) biosynthesis. But significantly, PhaJ1, the only R-ECH that is associated with PHA granules, was shown to be involved in PHA mobilization in this haloarchaeon. PhaJ1 catalyzes the dehydration of (R)-3-hydroxyacyl-CoA, the common product of PHA degradation, to enoyl-CoA, the intermediate of the ß-oxidation cycle, thus could link PHA mobilization to ß-oxidation pathway in H. mediterranei. This linkage was further indicated from the up-regulation of the key genes of ß-oxidation under the PHA mobilization condition, as well as the obvious inhibition of PHA degradation upon inhibition of the ß-oxidation pathway. Interestingly, 96% of phaJ-containing haloarchaeal species possess both phaC (encoding PHA synthase) and the full set genes of ß-oxidation, implying that the mobilization of carbon storage in PHA through the ß-oxidation cycle would be general in haloarchaea.


Assuntos
Proteínas Arqueais/metabolismo , Enoil-CoA Hidratase/metabolismo , Haloferax mediterranei/metabolismo , Poli-Hidroxialcanoatos/metabolismo , Acil Coenzima A/metabolismo , Sequência de Aminoácidos , Proteínas Arqueais/genética , Cromatografia Líquida de Alta Pressão , Enoil-CoA Hidratase/classificação , Enoil-CoA Hidratase/genética , Deleção de Genes , Regulação da Expressão Gênica em Archaea , Regulação Enzimológica da Expressão Gênica , Haloferax mediterranei/enzimologia , Haloferax mediterranei/genética , Isoenzimas/genética , Isoenzimas/metabolismo , Mutação , Oxirredução , Ácidos Pentanoicos/metabolismo , Filogenia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Homologia de Sequência de Aminoácidos
19.
Mar Genomics ; 28: 1-3, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26908308

RESUMO

Marinobacter psychrophilus strain 20041(T) was isolated from sea-ice of the Canadian Basin. Here we report the complete sequence of the 3.9-Mb genome of this strain. The complete genome sequence will facilitate the study of the physiology and evolution of Marinobacter species.


Assuntos
Genoma Bacteriano , Camada de Gelo/microbiologia , Marinobacter/genética , Regiões Árticas , Canadá , Marinobacter/classificação , Filogenia , Análise de Sequência de DNA
20.
Mar Genomics ; 26: 13-6, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26796622

RESUMO

Draconibacterium orientale strain FH5(T) isolated from a marine sediment sample from coast of Weihai, China, was a new species within the proposed new genus Draconibacterium in class Bacteroidia. Here, we present the genome sequence of D. orientale FH5(T), which contains 5,132,075 bp with a G+C content of 41.31%. The genome sequence will contribute to a better understanding of the physiology of this species.


Assuntos
Bactérias/genética , Genoma Bacteriano , Organismos Aquáticos , Mapeamento Cromossômico , Cromossomos Bacterianos , DNA Bacteriano/genética , Filogenia
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