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1.
J Environ Manage ; 261: 110226, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32148296

RESUMO

Dye decolorization is of crucial concern for effectively treating dye wastewater. In this study, rapid and effective decolorization of malachite green cationic dye was achieved by tea polyphenols and ferric ions under moderate conditions. Approximately 96.2% of decolorization efficiency could be obtained within the first 10 min at the initial dye concentration of 50 mg/L. The proposed method can perform excellently in a wide pH range of 5-9 and decolorization kinetics of malachite green under different solution pH were well fitted by the pseudo-second-order model. After the decolorization, only a slight reduction of tea polyphenols was observed, while the strength of peaks assigned to nitrogen-containing groups was significantly weakened, indicating that the N-demethylation reaction might occur during the decolorization process. The nucleophilic attack of deprotonated hydroxyl groups of tea polyphenols was proposed as the decolorization mechanism. The presence of ferric ions at an appropriate dosage could promote the deprotonation process and therefore enhance decolorization efficiency, while excess ferric ions in solution might compete with malachite green dye towards reductive sites on tea polyphenols. The findings from this study provided an economical and environmentally friendly technique for the effective decolorization of dye wastewater.


Assuntos
Polifenóis , Corantes de Rosanilina , Corantes , Concentração de Íons de Hidrogênio , Íons , Chá
2.
Chemosphere ; 252: 126378, 2020 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-32199161

RESUMO

Biofilm based systems and the hybrid between activated sludge and biofilms have been popularly applied for wastewater treatment. Unlike the suspended biomass, the biofilm concentration and kinetics on the media cannot be easily measured. In this study, a novel and easy-to-use approach has been developed based on pulse-flow respirometer to characterize the biofilm stoichiometry and kinetics in situ. With the new designed breathing reactor, the mutual interference between the magnetic stirring and biofilm media that happened in the conventional breathing reactor was solved. Moreover, Microsoft Excel based programs had been developed to fit the oxygen uptake rate curves with dynamic nonlinear regression. With this new approach, the yield coefficient, maximum oxidation capacity, and half-saturation constant of substrate for the heterotrophic biofilms in a fix bed reactor were determined to be 0.46 g-VSS/g-COD, 67.0 mg-COD/(h·L-media), and 4.4 mg-COD/L, respectively. Those parameters for biofilm ammonia oxidizers from a moving bed biofilm reactor were determined to be 0.17 g-VSS/g-N, 18.6 mg-N/(h·L-media), and 1.2 mg-N/L, respectively, and they were 0.11 g-VSS/g-N, 20.9 mg-N/(h·L-media), and 0.98 mg-N/L for nitrite oxidizers in the same biofilms. This study also found that the maximum specific substrate utilization rate for detached biofilms increased by 3.2 times, indicating that maintaining biofilm integrity was very important in the kinetic tests. Using this approach, the biofilm kinetics on the media can be regularly measured for treatment optimization.

3.
Comput Biol Med ; 119: 103653, 2020 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-32090899

RESUMO

BACKGROUND: Magnetic nanoparticles (MNPs) have been proposed as drug carriers for targeted therapy. Noninvasive imaging methods that can compute the distribution of MNPs have also attracted much attention. METHOD: Based on the Langevin theory, the theoretical relationship between the magnetic force and the concentration of MNPs was derived. The acoustic pressure wave equation containing the concentration of MNPs was established. RESULT: The acoustic pressure waveform reflected the dimension and position of the MNPs region. From reconstructed images, MNPs regions with different concentrations and different sizes were clearly distinguished. CONCLUSION: The concentration of MNPs can be parsed from the acoustic signals generated by particles vibrations. This conclusion indicates that magneto-acoustic concentration tomography of magnetic nanoparticles with magnetic induction (MACT-MI) has potential to detect and reconstruct the concentration of MNPs in biological tissue.

4.
Yeast ; 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-32050051

RESUMO

A highly efficient and targeted clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing system was constructed for Pichia pastoris (syn Komagataella phaffii). Plasmids containing single guide RNA and the methanol expression regulator 1 (MXR1) homology arms were used to precisely edit the transcriptional activator Mxr1 on the P. pastoris genome. At the S215 amino acid position of Mxr1, one, two, and three nucleotides were precisely deleted or inserted, and S215 was also mutated to S215A via a single-base substitution. Sequencing of polymerase chain reaction (PCR) amplicons in the region spanning MXR1 showed that CRISPR/Cas9 technology enabled efficient and precise gene editing of P. pastoris. The expression levels of several of the Mxr1-targeted genes, AOX1, AOX2, DAS1, and DAS2, in strains containing the various mutated variants of MXR1, were then detected through reverse transcription PCR following induction in methanol-containing culture medium. The frameshift mutations of Mxr1 led to almost zero transcription of AOX1, DAS1, and DAS2, while that of AOX2 was reduced to 60%. For the Mxr1 S215A mutant, the transcription of AOX1, AOX2, DAS1, and DAS2 was also reduced by nearly 60%. Based on these results, it is apparent that the transcription of AOX1, DAS1, and DAS2 is exclusively regulated by Mxr1 and serine phosphorylation at Mxr1 residue 215 is not critical for this function. In contrast, the transcription of AOX2 is mainly dependent on the phosphorylation of this residue. CRISPR/Cas9 technology was, therefore, successfully applied to the targeted editing of MXR1 on the P. pastoris genome, and it provided an effective method for the study of this transcription factor and its targets.

5.
Endocrine ; 2020 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-31927749

RESUMO

OBJECTIVES: To assess the efficacy and safety of once weekly glucagon-like peptide-1 receptor agonist (GLP-1 RA) dulaglutide for the treatment of type 2 diabetes mellitus (T2DM). MATERIALS AND METHODS: We searched PubMed, Embase, and Cochrane Library from inception to August 18, 2019. Revman5.3 and Stata13.0 software were used for meta-analysis. RESULTS: Twenty-one trials including 20,367 patients were analyzed. Compared with control group, hemoglobin A1c (HbA1c) in 0.75 mg dulaglutide group and 1.5 mg dulaglutide group were reduced by 0.29% and 0.55%, respectively. More patients treated with 0.75 mg dulaglutide [RR 1.24, 95% CI (1.08, 1.42), p = 0.002] and 1.5 mg dulaglutide [RR 1.66, 95% CI (1.40, 1.99), p < 0.00001] had reached the target of HbA1c 7.0%. In patients with T2DM, 0.75 mg dulaglutide and 1.5 mg dulaglutide had a statistically higher adverse events (AEs) incidence than control, whereas the risk of hypoglycaemia was lower in 0.75 mg dulaglutide group and 1.5 mg dulaglutide group than in control group. CONCLUSIONS: Based on the current evidence, 0.75 and 1.5 mg dulaglutide are associated with better glycemic control and lower rate of hypoglycemia in patients with T2DM.

6.
BMC Plant Biol ; 19(1): 546, 2019 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-31823718

RESUMO

BACKGROUND: As one of the main crops in the world, sterility of rice (Oryza sativa L.) significantly affects the production and leads to yield decrease. Our previous research showed that OsPUB73, which encodes U-box domain-containing protein 73, may be associated with male sterility. However, little information is available on this gene that is required for anther development. In the present study, we knocked out OsPUB73 by using the CRISPR/Cas9 system and studied the cytological and transcriptome of the gene-defect associated with pollen development and sterility in the rice variety (Taichung 65). RESULTS: The sequence analysis indicated that OsPUB73 was comprised of 3 exons and 2 introns, of which CDS encoded 586 amino acids including a U-box domain. The expression pattern of OsPUB73 showed that it was highly expressed in the anther during meiosis stage. The ospub73 displayed low pollen fertility (19.45%), which was significantly lower than wild type (WT) (85.37%). Cytological observation showed tapetum vacuolated at the meiosis stage and pollen exine was abnormal at the bi-cellular pollen stage of ospub73. RNA-seq analysis detected 2240 down and 571 up-regulated genes in anther of ospub73 compared with WT during meiosis stage. Among of 2240 down-regulated genes, seven known genes were associated with tapetal cell death or pollen exine development, including CYP703A3 (Cytochrome P450 Hydroxylase703A3), CYP704B2 (Cytochrome P450 Hydroxylase704B2), DPW (Defective Pollen Wall), PTC1 (Persistant Tapetal Cell1), UDT1 (Undeveloped Tapetum1), OsAP37 (Aspartic protease37) and OsABCG15 (ATP binding cassette G15), which were validated by quantitative real-time polymerase chain reaction (qRT-PCR). These results suggested OsPUB73 may play an important role in tapetal or pollen exine development and resulted in pollen partial sterility. CONCLUSION: Our results revealed that OsPUB73 plays an important role in rice male reproductive development, which provides valuable information about the molecular mechanisms of the U-box in rice male reproductive development.

7.
iScience ; 23(1): 100754, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31884171

RESUMO

The Hantzsch and free-radical polymerization reactions were combined in a one-pot high-throughput (HTP) system to simultaneously prepare 30 unique polymers in parallel. Six aldehydes derived from natural products were used as the starting materials to rapidly prepare the library of 30 poly(1,4-dihydropyridines). From this library, HTP evaluation methods led to the identification of an antibacterial polymer. Mechanistic studies revealed that the dihydropyridine group in the polymer side-chain structure plays an important role in resisting bacterial attachment to the polymer surface, thus leading to the antibacterial function of this polymer. This research demonstrates the value of multicomponent reactions (MCRs) in interdisciplinary fields by discovering functional polymers for possible practical applications. It also provides insights to further developing new functional polymers using MCRs and HTP methods with important implications in organic chemistry, polymer chemistry, and materials science.

9.
Sci Total Environ ; 694: 133625, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31756838

RESUMO

Thallium (Tl) contamination caused by the industrial wastewater leakage has become a serious environmental problem due to thallium's high toxicity. In this study, a novel titanium­iron magnetic nano-sized adsorbent was synthesized and applied for the effective removal of thallium(I). The physicochemical properties of the adsorbent were investigated by a series of techniques such as scanning electron microscope (SEM), X-ray powder diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). About 83% of equilibrium adsorption capacity could be accomplished within the initial 30 min. The adsorption of Tl(I) was found to be highly dependent on solution pH. The maximum adsorption capacity of Tl(I) was 111.3 mg/g at pH 7.0. The presence of such co-existing cations as Na+, Mg2+, Ca2+ and Cu2+ could have a certain influence on the uptake of Tl(I). The adsorption mechanism was proposed as a surface complexation process of Tl(I) ions by binding to deprotonated sites of hydroxyl groups on the adsorbent surface. The prepared magnetic adsorbent would be suitable for effectively treating thallium-containing water due to its promising adsorption ability towards Tl(I) and ease in operation.

10.
J Pharm Sci ; 108(12): 3785-3791, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31521642

RESUMO

Owing to the biological activity of the vaccine, the complicated production process, sterility, and uniformity of the product, the producing process of the vaccine is complicated and the product quality hard to control. In recent years, with the development of basic science such as cell biology, molecular biology, and metabolic engineering, bioprocess engineering research has developed rapidly. Therefore, U.S. Food and Drug Administration and European Medicines Agency conduct stringent control over the development of biomedical process engineering and product quality. This case study describes an example of Quality by Design-driven process development for manufacturing a human vaccine produced with Vero cells. Cell density in harvest fermentation broth and antigenic titer were chosen as 2 critical quality attributes. The study through 3 rounds design of experiment revealed that H2O2 and cell boost 4 had a significant effect on antigenic titer. Ethanolamine had significant improvement in the final concentration of cells. Through the Monte Carlo simulation, the design spaces and control space of process parameters were determined. A successful validation in a bioreactor was executed to verify the results of a spinner flask. Our investigation presents a successful case of Quality by Design principle, which encourages other researchers to combine the methodology into other biopharmaceutical manufacturing process.

11.
Mar Pollut Bull ; 146: 427-434, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31426177

RESUMO

To study the impact of rapid industrialization and urbanization in Guangxi Province on the coastal environment in recent years, seven metals (Hg, Cu, Pb, Cd, Cr, Zn and As) were investigated in the surface seawater and sediments of the northern Beibu Gulf. The levels of the metals were lower than in other regions in China, but Hg, Cu and Cd showed a significant increasing trend in both seawater and sediments over the past 20 years. Higher levels were consistently observed in the nearshore area, particularly in the northwest, which may be related to the rapid industrial development in coastal areas. Correlation and principal component analyses suggested that both terrestrial inputs and biological processes influenced the distribution of metals. In addition, the higher risk observed for Hg and Cu may be largely influenced by the increasing trend in these metals in the Beibu Gulf.


Assuntos
Sedimentos Geológicos/análise , Metais Pesados/análise , Água do Mar/análise , Poluentes Químicos da Água/análise , China , Monitoramento Ambiental , Urbanização
12.
Mar Pollut Bull ; 142: 603-612, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31232347

RESUMO

Dual isotope nitrate (NO3-) analysis was performed on the western coast of Guangdong Province to investigate seasonal changes in the main nitrate sources and their biogeochemical processing, which are due to the rapid development of the local economy. In the nearshore area, significant seasonal variations of nitrate sources occurred. The dominant nitrate sources, originating from manure and sewage, suggested that the nitrate along the west coast of Guangdong Province was mainly influenced by local cities despite the westward flow of diluted Pearl River water. In the offshore area, the nitrate loss in the upper and mid water during both two seasons mainly caused by phytoplankton assimilation, whereas coupled nitrification-denitrification could be responsible for the nitrate loss in the bottom waters during summer. Our results suggest that, with the rapid development of local economy, the nitrate sources in the coastal area have shifted to manure and sewage from the local cities.


Assuntos
Nitratos/análise , Poluentes Químicos da Água/análise , China , Desnitrificação , Monitoramento Ambiental/métodos , Fertilizantes , Esterco , Nitratos/metabolismo , Nitrificação , Isótopos de Nitrogênio/análise , Fitoplâncton/metabolismo , Estações do Ano , Água do Mar/análise , Água do Mar/química , Esgotos/análise
13.
Front Chem ; 7: 236, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31069214

RESUMO

A novel structural series of quinoline derivatives were designed, synthesized and biologically evaluated as PI3K/mTOR dual inhibitors upon incorporation of C-4 acrylamide fragment. Consequently, all of them exerted remarkable inhibition against PI3Kα with IC50 values ranging from 0.50 to 2.03 nM. Besides, they exhibited sub-micromolar to low micromolar anti-proliferative activity against both prostate cancer PC3 and colorectal cancer HCT116 cell lines. In subsequent profiling, 8i, a representative compound throughout this series, also significantly inhibited other class I PI3Ks and mTOR. In PC3 cells, it remarkably down-regulated the crucial biomarkers of PI3K/Akt/mTOR signaling, including phos-Akt (Ser473), phos-Akt (Thr308), phos-S6 ribosomal protein (Ser235/236), and phos-4E-BP1 (Thr37/46), at a concentration as low as 5 nM. Moreover, 8i displayed favorable metabolic stability with long elimination half-life in both human liver and rat liver microsomes. A further in vivo pharmacokinetic (PK) study demonstrated 8i possessed acceptable oral exposure, peak plasma concentration, and elimination half-life. Taken together, 8i, as a potent PI3K/mTOR dual inhibitor, merited further investigation and structural optimization.

14.
Small ; 15(21): e1900564, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30977978

RESUMO

Dip-pen nanolithography (DPN) is a unique nanofabrication tool that can directly write a variety of molecular patterns on a surface with high resolution and excellent registration. Over the past 20 years, DPN has experienced a tremendous evolution in terms of applicable inks, a remarkable improvement in fabrication throughput, and the development of various derivative technologies. Among these developments, polymer pen lithography (PPL) is the most prominent one that provides a large-scale, high-throughput, low-cost tool for nanofabrication, which significantly extends DPN and beyond. These developments not only expand the scope of the wide field of scanning probe lithography, but also enable DPN and PPL as general approaches for the fabrication or study of nanostructures and nanomaterials. In this review, a focused summary and historical perspective of the technological development of DPN and its derivatives, with a focus on PPL, in one timeline, are provided and future opportunities for technological exploration in this field are proposed.

15.
Pest Manag Sci ; 75(10): 2756-2764, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30859694

RESUMO

BACKGROUND: Poorly water-soluble and photosensitive pesticide compounds are difficult to be formulated as environmentally friendly formulations with high efficacy. Conventional wettable powder, emulsifiable concentrate and emulsion in water have disadvantages of dust drift, overuse of organic solvent and low efficacy. Therefore, there is an urgent need to construct a novel formulation to improve the bioavailability of pesticides. RESULTS: An abamectin nanosuspension was developed using a wet-milling method combined with orthogonal experimental design. The average particle sizes of the abamectin nanosuspension measured by dynamic light scattering, scanning electron microscope and transmission electron microscope were 233, 90 and 140 nm, respectively. The zeta potential and sliding angle on cabbage leaves were -36.9 mV and 62°. Retention and anti-photolysis were around 1.5 and 1.6 times those of emulsions in water. Furthermore, the biological activity of the nanosuspension towards diamondback moths was approximately twice that of conventional formulations. CONCLUSION: This study provides an easy and scalable technique for constructing pesticide nanosuspensions. The preparation and composition of the nanosuspension avoid the use of organic solvents. Application of the highly effective nanoformulation will significantly enhance pesticide efficacy, and reduce the dosage and environmental pollution of the pesticide. © 2019 Society of Chemical Industry.


Assuntos
Acaricidas/farmacocinética , Antinematódeos/farmacocinética , Inseticidas/farmacocinética , Ivermectina/análogos & derivados , Nanopartículas/química , Disponibilidade Biológica , Ivermectina/farmacocinética , Tamanho da Partícula , Solubilidade , Molhabilidade
16.
Chemistry ; 25(23): 5904-5911, 2019 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-30767346

RESUMO

Electrosynthesis of NH3 through the N2 reduction reaction (NRR) under ambient conditions is regarded as promising technology to replace the industrial energy- and capital-intensive Haber-Bosch process. Herein, a room-temperature spontaneous redox approach to fabricate a core-shell-structured Au@CeO2 composite, with Au nanoparticle sizes below about 10 nm and a loading amount of 3.6 wt %, is reported for the NRR. The results demonstrate that as-synthesized Au@CeO2 possesses a surface area of 40.7 m2 g-1 and a porous structure. As an electrocatalyst, it exhibits high NRR activity, with an NH3 yield rate of 28.2 µg h-1 cm-2 (10.6 µg h-1 mg-1 cat. , 293.8 µg h-1 mg-1 Au ) and a faradaic efficiency of 9.50 % at -0.4 V versus a reversible hydrogen electrode in 0.01 m H2 SO4 electrolyte. The characterization results reveal the presence of rich oxygen vacancies in the CeO2 nanoparticle shell of Au@CeO2 ; these are favorable for N2 adsorption and activation for the NRR. This has been further verified by theoretical calculations. The abundant oxygen vacancies in the CeO2 nanoparticle shell, combined with the Au nanoparticle core of Au@CeO2 , are electrocatalytically active sites for the NRR, and thus, synergistically enhance the conversion of N2 into NH3 .

17.
Rapid Commun Mass Spectrom ; 33(9): 883-893, 2019 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-30771236

RESUMO

RATIONALE: Discriminating between aglycone-substituted and saccharide-substituted saikosaponins by liquid chromatography/tandem mass spectrometry (LC/MSn ) is a long-standing issue that is still to be resolved. It is necessary to characterize the two types of substituted saikosaponins taking into consideration the potential significant difference in their bioactivity. METHODS: Taking the substituents malonyl and acetyl as examples, we developed a MS strategy to discriminate between the aglycone-substituted and saccharide-substituted saikosaponins through comparing their Y0 - nH2 O (n = 1-2) ions from the protonated molecules in the full-scan mass spectra and their B ions in the MS2 spectra of sodium-adduct molecules in the positive mode. RESULTS: The deprotonated molecules of the aglycone-substituted saikosaponins presented similar fragmentation patterns to those of saccharide-substituted ones in the negative mode, which could not discriminate whether the substitutes were located on the aglycone or the saccharide. In contrast, the Y0 - nH2 O (n = 1-2) ions containing or no substituent were observed respectively in the mass fragmentation of the protonated molecules of aglycone-substituted or saccharide-substituted saikosaponins in the positive mode. In addition, the B ions containing or no substituent were observed respectively in the mass fragmentation of the sodium-adduct molecules of the saccharide-substituted or aglycone-substituted saikosaponins in the positive mode. Two aglycone-malonylated saikosaponins were reported for the first time. CONCLUSIONS: Whether the substituents were located on the aglycone or the saccharide could be determined according to the Y0 - nH2 O (n = 1-2) ions from the protonated molecules in the full-scan mass spectra and the B ions in the MS2 spectra of sodium-adduct molecules in the positive mode. Our results have updated the mass fragmentation patterns of substituted saikosaponins, which is helpful for the quality control of pharmaceutical preparations containing saikosaponins. More importantly, this MS strategy should be able to be extended to characterize other substituted saponins of bioactive significance in future studies.

18.
Water Environ Res ; 91(6): 500-509, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30695122

RESUMO

Oxygen transfer is a key process determining the energy use of a biological wastewater treatment process. In this research, we investigated the effect of sludge morphology, especially the role of filamentous microorganisms, on oxygen transfer using bench-scale complete-mix activated sludge reactors with solids retention times (SRTs) of 10-, 20-, and 40-days, respectively. Results indicated 5%-10% reduced aeration need in the 40-day SRT reactor, compared with 10- and 20-day SRT reactors to maintain the same dissolved oxygen level, due to the improvement in sludge settleability and oxygen transfer efficiency (OTE). Filamentous microorganisms adversely impacted OTE via an increase in apparent viscosity of the mixed liquor, which resulted in an increase in the air bubble size and liquid film thickness and, therefore, limited oxygen transfer at the air-liquid boundary. A statistical analysis also confirmed that the mixed liquor viscosity is a statistically significant parameter links to OTE. PRACTITIONER POINTS: Filamentous organisms reduce oxygen transfer via increasing mixed liquor viscosity, which increases air bubble size and liquid film thickness at the air-liquid boundary. Increasing solids retention time reduces filament density. As a result, very long solids retention time promotes oxygen transfer.


Assuntos
Ar , Oxigênio/metabolismo , Esgotos/microbiologia , Reatores Biológicos/microbiologia , Esgotos/química , Viscosidade
19.
Nanomaterials (Basel) ; 9(2)2019 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-30678132

RESUMO

Recent years have witnessed significant progress in nanotechnology and pesticide research in pest control and crop protection. There are more motivations to develop nanoformulations that are less harmful to environment than conventional formulations. The use of nanosuspension has been proposed as a novel formulation to process poorly soluble pesticides. In this study, the lambda-cyhalothrin nanosuspension (LCNS) was prepared in a melt emulsification method. The prepared nanosuspension had a mean particle size of 12.0 ± 0.1 nm and a polydispersity index of 0.279 ± 0.135. The smaller particle size and polydispersity confer better wettability, stability and bioavailability than conventional suspension concentrates. The excellent properties of the nanosuspension were attributed to the reduced particle size and the emulsification and dispersion of the surfactants. The LCNS eliminates the need for organic solvents and significantly reduces the amount of surfactant required. The simple production process of LCNS saves production and equipment costs. The results indicate that lambda-cyhalothrin nanosuspensions would have a broad application prospect in agricultural production systems.

20.
Can J Physiol Pharmacol ; 97(5): 345-351, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-29894643

RESUMO

We aimed to investigate whether the cardioprotection of sevoflurane against ischemia-reperfusion (IR) injury is via inhibiting endoplasmic reticulum stress. The rat in vivo model of myocardial IR injury was induced by ligation of the left anterior descending coronary artery. Sevoflurane significantly ameliorated the reduced cardiac function, increased infarct size, and elevated troponin I level and lactate dehydrogenase activity in plasma induced by IR injury. Sevoflurane suppressed the IR-induced myocardial apoptosis. The increased protein levels of glucose-regulated protein 78 and C/EBP homologous protein (CHOP) after myocardial IR were significantly reduced by sevoflurane. The protein levels of phosphorylated protein kinase RNA-like endoplasmic reticulum kinase (PERK), phosphorylated eukaryotic initiation factor 2 (eIF2α), and activating transcription factor 4 (ATF4) were significantly increased in rats with IR and attenuated by sevoflurane treatment. The phosphorylation of Akt was further activated by sevoflurane. The cardioprotection of sevoflurane could be blocked by wortmannin, a PI3K/Akt inhibitor. Our results suggest that the cardioprotection of sevoflurane against IR injury might be mediated by suppressing PERK/eIF2a/ATF4/CHOP signaling via activating the Akt pathway, which helps in understanding the novel mechanism of the cardioprotection of sevoflurane.


Assuntos
Cardiotônicos/farmacologia , Estresse do Retículo Endoplasmático/efeitos dos fármacos , Traumatismo por Reperfusão Miocárdica/patologia , Traumatismo por Reperfusão Miocárdica/prevenção & controle , Sevoflurano/farmacologia , Fator 4 Ativador da Transcrição/metabolismo , Animais , Apoptose/efeitos dos fármacos , Vasos Coronários/efeitos dos fármacos , Vasos Coronários/patologia , Fator de Iniciação 2 em Eucariotos/metabolismo , Coração/efeitos dos fármacos , Coração/fisiopatologia , Masculino , Traumatismo por Reperfusão Miocárdica/metabolismo , Traumatismo por Reperfusão Miocárdica/fisiopatologia , Miocárdio/metabolismo , Miocárdio/patologia , Proteínas Proto-Oncogênicas c-akt/metabolismo , Ratos , Ratos Sprague-Dawley , Transdução de Sinais/efeitos dos fármacos , eIF-2 Quinase/metabolismo
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