Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 17 de 17
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Curr Microbiol ; 2020 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-31982968

RESUMO

The soil organic carbon is associated with the plant quality and the microbial community structure. In the present study, carbon fertilizers were applied to paddy soil to elucidate the relationship between soil carbon and neutral aroma substances in both tobacco and soil microbiome by transcriptome sequencing and 16S rDNA-based analysis, respectively. Our results showed that (1) the increase in soil carbon content was closely correlated with the abundance of microorganisms belonging to two classes (which could potentially affect tobacco plants), namely Gammaproteobacteria and Chloroflexia, (2) soil carbon apparently affected tobacco neutral aroma substances, and (3) soil carbon improved neutral aroma substances by affecting the transcriptional processes of sesquiterpenoid and chlorophyll biosyntheses. These results suggest that increased soil carbon-especially active organic carbon-resulted in desirable improvements in aroma substances in tobacco leaves.

2.
PLoS One ; 14(10): e0224556, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31671156

RESUMO

The application of biochar is one of the most useful methods for improving soil quality, which is of the utmost significance for the continuous production of crops. As there are no conclusive studies on the specific effects of biochar application on tobacco quality, this study aimed to improve the yield and quality of tobacco as a model crop for economic and genetic research in southern China, by such application. We used transcriptome sequencing to reveal the effects of applied biochar on tobacco development before and after topping. Our results showed that topping affected carbon and nitrogen metabolism, photosynthesis and secondary metabolism in the tobacco plants, while straw biochar-application to the soil resulted in amino acid and lipid synthesis; additionally, it affected secondary metabolism of the tobacco plants through carbon restoration and hormonal action, before and after topping. In addition to the new insights into the impact of biochar on crops, our findings provide a basis for biochar application measures in tobacco and other crops.

3.
PeerJ ; 7: e7576, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31565561

RESUMO

Background: The increasing demand for food production has resulted in the use of large quantities of chemical fertilizers. This has created major environmental problems, such as increased ammonia volatilization, N2O emission, and nitrogen (N) leaching from agricultural soil. In particular, the utilization rate of N fertilizer is low in subtropical southern parts of China due to high rainfall. This causes not only large financial losses in agriculture, but also serious environmental pollution. Methods: In this study, 16S rDNA-based analysis and static-chamber gas chromatography were used to elucidate the effects of continuous straw biochar application on the N pool and bacteria environment in two typical soil types, purple and paddy soils, in southern China. Results: Straw biochar application (1) improved the soil N pool in both rhizosphere and non-rhizosphere soils; (2) significantly reduced the emission of N2O, with no difference in emission between 1 and 2 years of application; (3) increased the abundance of N-processing bacteria in the soil and altered the bacterial community structure; and (4) improved the tobacco yield and N use efficiency in paddy soil. These findings suggest that, in southern China, the application of straw biochar can promote N transformation in purple and paddy soils and reduce the emission of the greenhouse gas N2O.

4.
R Soc Open Sci ; 6(7): 181499, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31417689

RESUMO

Soil carbon reserves are the largest terrestrial carbon pools. Common agricultural practices, such as high fertilization rates and intensive crop rotation, have led to global-scale environmental changes, including decreased soil organic matter, lower carbon/nitrogen ratios and disruption of soil carbon pools. These changes have resulted in a decrease in soil microbial activity, severe reduction in soil fertility and transformation of soil nutrients, thereby causing soil nutrient imbalance, which seriously affects crop production. In this study, 16S rDNA-based analysis and static chamber-gas chromatography were used to elucidate the effects of continuous application of straw biochar on soil carbon pools and the soil microbial environments of two typical soil types (purple and paddy soils) in southern China. Application of biochar (1) improved the soil carbon pool and its activity, (2) significantly promoted the release of soil CO2 and (3) improved the soil carbon environment. Soil carbon content was closely correlated with the abundance of organisms belonging to two orders, Lactobacillales and Bacteroidales, and, more specifically, to the genus Lactococcus. These results suggest that biochar affects the soil carbon environment and soil microorganism abundance, which in turn may improve the soil carbon pool.

5.
Sci Rep ; 9(1): 6168, 2019 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-30992508

RESUMO

The application of fertilisers incorporated with plant residues improves nutrient availability in soils, which shifts the microbial community structure and favours plant growth. To understand the impact of wheat straw compost fertiliser on soil properties and microbial community structure, tobacco planting soils were treated with four different fertilisers using varied amounts of straw compost fertiliser and a no fertiliser control (CK). Results showed that different fertilisers affected available soil nutrient contents differently. Treatment of tobacco soil with application of combined chemical fertiliser/wheat straw compost led to improved soil chemical properties, and increased soil organic matter and available phosphorus and potassium content. Treatment with FT1 200 kg/mu straw was found to be superior in improving soil fertility. Metagenomic DNA sequencing revealed that different fertiliser treatments resulted in changes in the microbial community composition. In soil treated with FT2 300 kg/mu straw for 60 days, the predominant bacterial phyla were Proteobacteria, Actinobacteria, and Verrucomicrobia, whereas Cyanobacteria, Basidiomycota, and Chlorophyta were found in high abundance in soil samples treated with FT1 200 kg/mu straw for 30 days. Functional annotation of metagenomic sequences revealed that genes involved in metabolic pathways were among the most abundant type. PCoA analysis clearly separated the samples containing straw compost fertiliser and chemical fertiliser. A significant correlation between soil properties and the dominant phyla was identified.

6.
Huan Jing Ke Xue ; 40(2): 915-923, 2019 Feb 08.
Artigo em Chinês | MEDLINE | ID: mdl-30628360

RESUMO

A five year (2013-2017) experiment was conducted to explore the effects of biochar application on the dynamic changes in soil respiration, soil water, and heat factors under four treatments:CK (without biochar), T1 (with 1.5 t·hm-2biochar), T2 (with 15 t·hm-2biochar), and T3 (with 45 t·hm-2biochar). The results showed that:① the soil respiration rate in the growing season of flue-cured tobacco was significantly reduced by 25.89% under the five year application of medium-dose biochar (T2:15 t·hm-2) in soil, while it was significantly increased by 21.48% when the applied dose increased to 45 t·hm-2(T3) (P<0.05). ② The long-term application of medium-dose biochar in the soil significantly reduced the soil heterotrophic respiration and autotrophic respiration rates by 29.80% and 28.75%, respectively. Meanwhile, the application of high-dose biochar (T3:45 t·hm-2) significantly increased the heterotrophic respiration rate by 28.88%. In addition, the application of low-dose biochar (T1:1.5 t·hm-2) and medium-dose biochar significantly increased the proportion of autotrophic respiration, whereas the high-dose biochar application significantly increased the proportion of heterotrophic respiration (P<0.05). ③ The addition of low-dose biochar to the soil significantly reduced the soil temperature at 5 cm in the growing season of flue-cured tobacco, while the high-dose application significantly reduced the soil humidity. There was a significant index correlation between soil respiration and soil temperature at 5 cm but no significant correlation with soil humidity at 5 cm (P<0.05). Ultimately, the application of low-dose biochar for five years in soil had no effect on soil respiration, and the application of the proper amount of biochar had a carbon sequestration effect. Additionally, large-dose biochar application may be counterproductive. It is recommended that the application range of biochar should be controlled within 15 t·hm-2.


Assuntos
Carvão Vegetal , Solo/química , Tabaco/crescimento & desenvolvimento , Sequestro de Carbono
7.
Huan Jing Ke Xue ; 39(5): 2412-2419, 2018 May 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965542

RESUMO

To identify the effect of biochar addition on soil abiotic and biotic properties and provide evidence for the soil improvement with biochar input, the soil physiochemical properties and fungal community were investigated in a cinnamon soil after 3-year biochar additions of 10, 20, and 40 t ·hm-2. The relationship between the fungal community and edaphic physicochemical characteristics was also analyzed. The results showed that soil pH, moisture, total nitrogen (TN), and total organic carbon (TOC) significantly increased but dissolved organic carbon (DOC) content and soil bulk density decreased with biochar addition. High-throughput sequencing results indicated that biochar amendment had little influence on fungal α diversity but significantly changed the fungal community structure. The taxonomic classification showed that the dominant fungal phyla were Ascomycota, Zygomycota, and Basidiomycota, and these phyla accounted for more than 90% of the total sequences. The relative abundance of Ascomycota and Basidiomycota increased, while the abundance of Zygomycota decreased with biochar addition. At the genus level, biochar addition increased the relative abundances of Alternaria, Conocybe, and Aspergillus but decreased the relative abundances of Actinomucor and Gibberella. Redundancy analysis (RDA) showed that soil DOC, pH, and moisture were key environmental factors leading to the shift in the soil fungal community composition. In summary, the application of biochar changed the soil physicochemical properties, which drove the ecological succession of soil fungal communities.


Assuntos
Carvão Vegetal/química , Fungos/classificação , Microbiologia do Solo , Solo/química
8.
Res Microbiol ; 169(2): 115-126, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29122672

RESUMO

Soil microorganisms play a crucial role in cycling soil nutrients and providing organic nutrients for plant growth and development. Fertilisation balances soil fertility and quality, and affects soil microbial communities. Fertilisation is a frontier subject in agricultural and environmental sciences. Here we showed that the application of high-carbon basal fertiliser treatment could improve the tobacco yield and quality when compared to chemical fertiliser, high-carbon basal fertiliser and mixed high-carbon chemical fertiliser. The potential reason is that different fertiliser treatments influence soil fertility, such as nitrogen, phosphorus, and other contents, besides soil organic matter. Further experiments revealed that populations of bacteria, fungi and actinomycetes fluctuated during tobacco development under different fertilisation treatments. Then we performed high-throughput sequencing of the 16S rRNA gene, and the results showed that the fertilisation treatments had significant effects on the microbial community, particularly within the finer taxonomic divisions or non-dominant taxa. Moreover, proteobacteria and fungal genera had significantly different relative abundances during tobacco growth under various tobacco developmental stages and fertilisation treatments. These results indicated that mixed high-carbon chemical fertiliser could improve soil fertility by influencing the soil microorganism, and that the fertilisation treatments impacted on the structure and composition of the microbial community, and especially the diversity of non-dominant taxa. However, more studies are needed to confirm their reliability.


Assuntos
Bactérias/isolamento & purificação , Carbono/análise , Fertilizantes/análise , Fungos/isolamento & purificação , Microbiologia do Solo , Tabaco/crescimento & desenvolvimento , Bactérias/classificação , Bactérias/genética , Bactérias/metabolismo , Biodiversidade , Carbono/metabolismo , Fungos/classificação , Fungos/genética , Fungos/metabolismo , Fósforo/análise , Fósforo/metabolismo , Solo/química , Tabaco/microbiologia
9.
Ying Yong Sheng Tai Xue Bao ; 26(5): 1440-6, 2015 May.
Artigo em Chinês | MEDLINE | ID: mdl-26571663

RESUMO

Using 'Yuyan 10' as the material, the effects of different phosphorus fertilizer application on root characteristics of tobacco, such as root dry mass and the difference of dry matter distribution and mineral nutrient accumulation between its above and underground parts were investigated. The results showed that the growth of flue-cured tobacco root system and the distribution of dry matter to the aboveground part were significantly promoted by phosphorus fertilizer application. The application of 30 kg P2O5 · hm(-2) led to the maximums of root dry mass, root volume, root activity and the minimum of root to shoot ratio. The maximum nutrient accumulation rates of root and leaf appeared 57-66 days after transplanting and 44-55 days after transplanting, respectively. Phosphorus could not only promote the mineral nutrition absorption of tobacco and the earlier appearance of maximum nutrient accumulation, but significantly promote the nutrient accumulation of the aboveground part. But, the positive effects described above would be weakened when the amount of phosphorus fertilizer was more than 30 kg P2O5 · hm(-2). Therefore, it's necessary to control the amount of phosphorus application to improve the quality of tobacco leaves.


Assuntos
Fertilizantes , Fósforo/química , Raízes de Plantas/crescimento & desenvolvimento , Tabaco/fisiologia , Folhas de Planta/crescimento & desenvolvimento
10.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 40(12): 1357-64, 2015 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-26739079

RESUMO

OBJECTIVE: To evaluate signal intensity-time (SI-Time) curve and quantitative dynamic contrast-enhanced 3.0T magnetic resonance imaging in diagnosis and differentiating neoplasm of uterus.
 METHODS: A total of 42 cases of uterine neoplasm (20 were malignant and 22 were benign) were evaluated in our study. All cases received dynamic contrast-enhanced scanning on 3.0T MRI. The raw data was processed by Siemens Tissue 4D software and the SI-Time curve was obtained and analyzed. Pharmacokinetic modeling of Tofts with a modeled vascular input function was used for calculating volume parameters: volume transfer constant (Ktrans), reverse volume transfer constant (Kep), the extravascular extracellular space volume per unit volume of tissue (Ve). The correlation of these parameters at each groups were investigated. The SI-Time curve and the data of perfusion parameters between the 2 groups were compared by T test.
 RESULTS: Among 20 malignant tumors, 12 were cervical carcinoma and 8 were endometrial cancer. Among the benign tumors, 13 were leiomyomas, 3 were endometrial polyp, 3 were endometrial hyperplasia, and 3 were adenomyosis. 59.1% cases of benign tumors belong to Type I curve and 65% cases of malignant tumors belong to Type II curve. There was significant difference in SI-Time curve between benign and malignant tumors (P=0.011). If Type I curve was used as diagnostic criteria for benign tumors, and Type II and III curve were for malignant tumors, the diagnostic sensitivity, specificity, positive predictive value, negative predictive value were 90.0%, 59.1%, 66.7%, and 86.7%, respectively. Ve was 0.477 ± 0.143 in malignant and 0.614 ± 0.146 in control group with significant difference (P=0.004). Ve was 0.477 ± 0.143 in malignant and 0.589 0.176 in benign group with significant difference (P=0.004). Ktrans was (0.178 ± 0.067) min⁻¹ in malignant and (0.263 ± 0.111) min⁻¹ in control group with significant difference (P=0.003). Ktrans was (0.182 ± 0.096) min⁻¹ in benign and (0.263 ± 0.111) min⁻¹ in control group with significant difference (P=0.011). 
 CONCLUSION: The type of SI-Time curve and perfusion parameters were important for differentiating benign and malignant uterine tumors in dynamic enhanced MRI. These parameters provide a supplement for conventional morphological MR diagnosis.


Assuntos
Meios de Contraste , Imagem por Ressonância Magnética , Neoplasias Uterinas/diagnóstico , Útero/patologia , Feminino , Humanos , Sensibilidade e Especificidade
11.
Mol Biol Rep ; 41(9): 5701-9, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24919756

RESUMO

SnRK2 is a plant-specific protein kinase family involved in abiotic stress signalling. In this study, NtSnRK2.1, NtSnRK2.2, and NtSnRK2.3, were cloned from tobacco by in silico cloning and reverse transcription PCR. The three protein kinases were classed into subfamily II of the SnRK2 family using a phylogenetic tree and C-terminus analysis. Subcellular localization revealed NtSnRK2s in the nuclear and cytoplasmic compartments. Dynamic expression of NtSnRK2s in tobacco plants that were exposed to drought, salt, or cold stressors were characterised using quantitative real-time PCR. It was revealed that the three genes showed similar patterns of transcription under abiotic stress responses; there was evidence NtSnRK2s participated in abscisic acid-dependent signalling pathways. NtSnRK2.1-3 responded much faster to drought and salt than to cold stress. To investigate the role of NtSnRK2s under abiotic stresses, NtSnRK2.1 gene was over-expressed in tobacco. A stress tolerance assay showed that tobacco plants that over-expressed NtSnRK2.1 plants had greater salt tolerance. The results indicate that NtSnRK2s are involved in abiotic stress response pathways.


Assuntos
Proteínas de Plantas/genética , Proteínas Serina-Treonina Quinases/genética , Tabaco/genética , Sequência de Aminoácidos , Compartimento Celular , Clonagem Molecular , Temperatura Baixa , Citoplasma/metabolismo , DNA Complementar/genética , DNA de Plantas/genética , Secas , Dados de Sequência Molecular , Filogenia , Proteínas de Plantas/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Análise de Sequência de DNA , Transdução de Sinais , Cloreto de Sódio , Estresse Fisiológico , Tabaco/química
12.
PLoS One ; 8(4): e60279, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23593186

RESUMO

OBJECTIVE: Prospectively assess the performance of diffusion-weighted magnetic resonance imaging (DW-MRI) for differentiation of central lung cancer from atelectasis. MATERIALS AND METHODS: 38 consecutive lung cancer patients (26 males, 12 females; age range: 28-71 years; mean age: 49 years) who were referred for thoracic MR imaging examinations were enrolled. MR examinations were performed using a 1.5-T clinical scanner and scanning sequences of T1WI, T2WI, and DWI. Cancers and atelectasis were measured by mapping of the apparent diffusion coefficients (ADCs) obtained with a b-value of 500 s/mm(2). RESULTS: PET/CT and DW-MR allowed differentiation of tumor and atelectasis in all 38 cases, but T2WI did not allow differentiation in 9 cases. Comparison of conventional T2WI and DW-MRI indicated a higher contrast noise ratio of the central lung carcinoma than the atelectasis by DW-MRI. ADC maps indicated significantly lower mean ADC in the central lung carcinoma than in the atelectasis (1.83±0.58 vs. 2.90±0.26 mm(2)/s, p<0.0001). ADC values of small cell lung carcinoma were significantly greater than those from squamous cell carcinoma and adenocarcinoma (p<0.0001 for both). CONCLUSIONS: DW-MR imaging provides valuable information not obtained by conventional MR and may be useful for differentiation of central lung carcinoma from atelectasis. Future developments may allow DW-MR imaging to be used as an alternative to PET-CT in imaging of patients with lung cancer.


Assuntos
Carcinoma/diagnóstico , Imagem de Difusão por Ressonância Magnética , Neoplasias Pulmonares/diagnóstico , Imagem Multimodal , Tomografia por Emissão de Pósitrons , Atelectasia Pulmonar/diagnóstico , Tomografia Computadorizada por Raios X , Adulto , Idoso , Diagnóstico Diferencial , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Carcinoma de Pequenas Células do Pulmão
13.
J Plant Res ; 126(1): 121-9, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22760586

RESUMO

Leaf morphology and the leaf protein expression profiles of flue-cured tobacco grown in central Henan province of China under low nitrogen (low-N) and normal nitrogen (normal-N) nutrition were examined. The leaf length and width were measured at 50, 60, and 70 days after transplanting. Leaves grown under low-N conditions were shorter and more narrow than those grown under normal-N conditions. The protein expression profiles of tobacco leaves harvested at 70 days after transplanting were analyzed by 2-dimensional electrophoresis, and five differentially expressed proteins including a putative protein were identified. Except for the MCM protein-like protein, the other three differentially expressed proteins of cyclophilin-like protein, vacuolar invertase INV2, MAR-binding protein and the one putative protein showed increased expression in the low-N nutrition group. Among these proteins, the cyclophilin-like protein, which is a stress-responsive signal protein, may play pivotal roles in regulating leaf development under stress conditions. Real-time quantitative PCR analysis showed that the mRNA expression level of the cyclophilin-like protein at day 50, 60 and 70 under low-N conditions was 0.90, 1.43 and 6.9-fold higher than that under normal-N conditions, indicating that the gene expression of cyclophilin-like protein was strongly induced by low-N conditions.


Assuntos
Ciclofilinas/metabolismo , Nitrogênio/deficiência , Nitrogênio/metabolismo , Folhas de Planta/crescimento & desenvolvimento , Tabaco/metabolismo , Fertilizantes , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Folhas de Planta/anatomia & histologia , Proteínas de Plantas/metabolismo , RNA Mensageiro/metabolismo , Transdução de Sinais , Estresse Fisiológico , Tabaco/genética
14.
Ying Yong Sheng Tai Xue Bao ; 21(8): 2072-7, 2010 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-21043118

RESUMO

Taking flue-cured tobacco Yunyan 87 as test material, this paper studied its photosynthesis and dry matter production at seedling stage under 100%, 88%, 72%, and 62% natural light intensities. At noon of sunny days, 100% natural light intensity inhibited the photosynthesis, while proper shading (88% natural light intensity) could eliminate the inhibition, and the daily photosynthesis was significantly higher than other treatments. Shading reduced the light saturation point and compensation point, enhanced the apparent quantum yield of photosynthesis and the net photosynthetic rate under weak light, increased the chlorophyll a and chlorophyll b contents, but decreased the chlorophyll a/b and cartenoids contents. Under 88% natural light intensity, tobacco seedlings had higher light saturation point, lower compensation point, higher suitability to the change of light intensity, and higher photosynthetic potentiality. 100% natural light intensity was more advantageous to the transfer of dry matter and soluble sugar to stem, while 88% natural light intensity was more beneficial to the transfer of dry matter and soluble sugar to root. Under the conditions of this experiment, proper shading (88% natural light intensity treatment) could improve the seedling quality of flue-cured tobacco.


Assuntos
Carboidratos/análise , Fotossíntese/fisiologia , Plântula , Luz Solar , Tabaco/fisiologia , Plântula/crescimento & desenvolvimento , Plântula/fisiologia , Plântula/efeitos da radiação , Tabaco/crescimento & desenvolvimento , Tabaco/efeitos da radiação
15.
Wei Sheng Wu Xue Bao ; 49(5): 624-30, 2009 May.
Artigo em Chinês | MEDLINE | ID: mdl-19637570

RESUMO

OBJECTIVE: We screened dominating microbial species isolated from aging flue-cured tobacco and studied their aroma improving effect. METHODS: Total DNA of microorganisms from the fermentation flue-cured tobacco surface of NC89, ZhongYan 100 and ZhongYan 101 were extracted. Under the PCR-DGGE, the diversity of microorganisms on fermentation tobacco leaves were studied and dominating microbial species were screened. We further studied the influence of dominating microbial species on the content of aroma components of the fermentation flue-cured tobacco. RESULTS: 1) By using DGGE analysis, there were 5 dominant bands A, B, C, D and E in all tobacco leaves samples of the three varieties; In further studies, five dominant DGGE bands were isolated, cloned and sequenced. From them we screened a dominant microorganism. 2) The content of most aroma components in tobacco leaves increased when they were sprayed with the dominant microorganism, comparing with the control. CONCLUSION: The dominant microorganism can improve the flavor of tobacco leaves during ripening.


Assuntos
Bactérias/metabolismo , Fermentação/fisiologia , Folhas de Planta/microbiologia , Tabaco/microbiologia , Técnicas de Laboratório Clínico , Hidrocarbonetos Aromáticos , Fenômenos Fisiológicos , Folhas de Planta/química , Tabaco/genética , Indústria do Tabaco/métodos
16.
Ying Yong Sheng Tai Xue Bao ; 18(11): 2642-5, 2007 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-18260477

RESUMO

Through shading with white gauze to simulate different light habitats (100%, 68.2% 35.4% and 16.7% of full sunlight), this paper studied the effects of light intensity on the morphological and physiological characteristics of tobacco seedlings. The results showed that with the decrease of relative light intensity, seedling height increased, while stem circumference, dry weight/fresh weight ratio, leaf thickness, specific leaf weight, and matter accumulation decreased. Less effect of light intensity was observed on the number of seedling leaves. Under weak light condition, the contents of free water, chlorophyll, total nitrogen, and protein in leaves increased, while bound water content, chlorophyll a/b ratio, and invertase activity decreased. The root/shoot ratio, root biomass, and root vitality also decreased. All of these suggested that weak light was unfavorable to the cultivation of strong tobacco seedlings, and the light conditions of seedbed should be improved in tobacco production.


Assuntos
Folhas de Planta/efeitos da radiação , Plântula/efeitos da radiação , Luz Solar , Tabaco/efeitos da radiação , Clorofila/metabolismo , Nitrogênio/metabolismo , Folhas de Planta/anatomia & histologia , Folhas de Planta/fisiologia , Proteínas de Plantas/metabolismo , Plântula/anatomia & histologia , Plântula/fisiologia , Tabaco/anatomia & histologia , Tabaco/fisiologia , Água/metabolismo
17.
Se Pu ; 24(2): 161-3, 2006 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-16830465

RESUMO

A reversed-phase high performance liquid chromatographic (RP-HPLC) method for the determination of carotenoids in flue-cured tobacco leaves was developed. Carotenoids were extracted from flue-cured tobacco leaves by acetone-water (90:10, v/v) solution containing 0.1% butylated hydroxytoluene (BHT). Plant proteins were eliminated by adding 0.1 g Pb(Ac)2 and by centrifugation (10000 r/min) for 5 min at 4 degrees C. Lutein, beta-carotene, neoxanthin, violaxanthin and other plant pigments were separated on a reversed-phase C18 column (3.9 mm i.d. x 150 mm, 5 microm), with a mobile phase of (A) methanol-isopropyl alcohol (1:1, v/v) and (B) water using a gradient elution at a flow rate of 0.5 mL/min. The optimum elution gradient was as follows: 0-10 min, 70% A + 30% B; 10-17 min, 100% A; 17-30 min, 90% A + 10% B. The recoveries of carotenoids in flue-cured tobacco leaves were 91.77%-97.42%, and relative standard deviations were 3. 46%-0.98%. This method was applied to determine carotenoids in flue-cured tobacco leaves during its growth with satisfactory results.


Assuntos
Carotenoides/análise , Cromatografia Líquida de Alta Pressão/métodos , Cromatografia de Fase Reversa/métodos , Tabaco/química , beta Caroteno/análise , Luteína/análise , Xantofilas/análise
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA