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1.
Int Immunopharmacol ; 82: 106316, 2020 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-32088642

RESUMO

Hydroxysafflor yellow A (HSYA) is an effective therapeutic agent that alleviates myocardial ischaemia/reperfusion injury (MIRI), but the exact mechanisms remain elusive. The aim of this study was to investigate the potential protective effect of HSYA against MIRI through mechanisms related to NLRP3 inflammasome regulation. In this study, hypoxia/reoxygenation (H/R)-induced H9c2 cardiomyocytes were treated with HSYA or the AMPK inhibitor, compound C (CC). Our results showed that HSYA pretreatment improved cardiomyocyte viability, maintained mitochondrial membrane potential, reduced apoptotic cardiomyocytes, decreased caspase-3 activity, and inhibited NOD-like receptor 3 (NLRP3) inflammasome activation during H/R injury. Moreover, the inhibition of AMPK activation by the CC inhibitor partially abolished the effects of HSYA treatment, including suppressing the upregulation of NLRP3 inflammasome components (NLRP3, caspase-1 and interleukin-1ß) and promoting autophagy (LC3-II/LC3-I and p62). In conclusion, the protective mechanism of HSYA in H/R-induced cardiomyocyte injury is associated with inhibiting NLRP3 inflammasome activation through the AMPK signalling pathway.

2.
J Plant Physiol ; 244: 153093, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31841951

RESUMO

Nitrogen (N) mobilization from mature leaves plays a key role in supplying amino acids to vegetative and reproductive sinks. However, it is unknown if the mobilized N is predominantly sourced by net N-export (a senescence-related process) or other source of N-export from leaves. We used a new approach to partition gross and net N-export from leaf blades at different developmental stages in Cleistogenes squarrosa (a perennial C4 grass). Net N-export was determined as net loss of leaf N with age, while gross N-export was quantified from isotopic mass balances obtained following 24 h-long 15N-labeling with nitrate on 10-12 developmentally distinct (mature and senescing) leaves of individual major tillers. Net N-export was apparent only in older leaves (leaf no. > 7, with leaves numbered basipetally from the tip of the tiller and leaf no. 2 the youngest fully-expanded leaf), while gross N-export was largely independent of leaf age category and was ∼8.4 times greater than the net N-export of a tiller. At whole-tiller level, N import compensated 88 ±â€¯14 (SE) % of gross N-export of all mature blades leading to a net N-export of 0.51 ±â€¯0.07 (SE) µg h-1 tiller-1. N-import was equivalent to 0.09 ±â€¯0.01 (SE) d-1 of total leaf N, similar to reported rates of leaf protein turnover. Gross N-export from all mature blades of a tiller was ∼1.9-times the total demand of the immature tissues of the same (vegetative) tiller. Significant N-export is evident in all mature blades, and is not limited to senescence conditions, implying a much shorter mean residence time of leaf N than that calculated from net N-export. Gross N-export contributes not only to the N demand of the immature tissues of the same tiller but also to N supply of other sinks, such as newly formed tillers. N dynamics at tiller level is integrated with that of the remainder of the shoot, thus highlights the importance of integration of leaf-, tiller-, and plant-scale N dynamics.

3.
Huan Jing Ke Xue ; 41(1): 460-468, 2020 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854949

RESUMO

The effects of a single application of three amendments (biochar, lignite, and chicken manure) on the fraction transformations of soil Cd and Ni and uptake by winter wheat are reported to provide reference for passivation and the remediation of heavy metals in soil. Field experiments were conducted to investigate the effects of different passivators on the forms of Cd and Ni in soils at different growth stages of winter wheat, and to analyze the contents of Cd and Ni in different organs of wheat. The results showed that biochar increased soil pH and that lignite reduced soil pH, but not significantly. Chicken manure significantly reduced soil pH at booting and mature stages, and decreased by 0.23 and 0.20 pH units, respectively. The single application of biochar, chicken manure, or lignite did not reduce the exchangeable Ni content significantly, whereas the effect on the exchangeable Cd was significant. Lignite 2% treatment had the greatest decrease in exchangeable Cd at different growth stages of wheat, which were 30.50%, 43.34%, and 31.20%, respectively. The contents of Cd and Ni in the shoots and underground parts of wheat decreased to some extent, and the extent of these decreases increased with the increase of passivator dosage. The decrease of Cd content in wheat roots was the largest under the treatment of lignite 2% at different growth stages, reaching 38.35%, 58.00%, and 50.20% respectively. The greatest decline of Ni content in wheat roots occurred in the lignite 2% treatment at the booting (41.33% decline) and mature stages (51.35%). All the three amendments reduced the availability of Cd and Ni in slightly alkaline soil, and the passivation effect on Cd was better than that of Ni. All three amendments also effectively reduced the content of Cd and Ni in different organs of wheat plants in different growth period. The order of decreasing effect of Cd in wheat organs was lignite > biochar > chicken manure at the same dose of these three passivators.

4.
Plant Sci ; 290: 110293, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31779893

RESUMO

Class I TGA transcription factors (TFs) are known to participate in plant resistance responses, however, their regulatory functions in the biosynthesis of secondary metabolites were rarely revealed. In this study, a class I TGA TF, TwTGA1, from Tripterygium wilfordii Hook.f. was cloned and characterized. Overexpression of TwTGA1 in T. wilfordii Hook.f. cells increased the production of triptolide and two sesquiterpene pyridine alkaloids, which was further enhanced by methyl jasmonate (MeJA) treatment. RNA interference of TwTGA1 showed no significant effects on the production of these metabolites, indicating the existence of other TGA partner(s) with overlapping functions. Heterologous expression of TwTGA1 in tobacco By-2 cells promoted the biosynthesis of pyridine alkaloids. Under the elicitation of MeJA, the contents of nonpyrrolidine alkaloids further increased but not for nicotine. TwTGA1 could induce the expression of Putrescine N-methyltransferase (PMT) and N-methylputrescine oxidase 1 (MPO1) through binding to their promoters. Finally, transient expression of TwTGA1 in leaves of Catharanthus roseus changed both the profiles of vinca alkaloids (increased contents of serpentine and catharanthine, but decreased that of vinblastine) and the expressions of biosynthesis-related genes. The metabolic and transcriptional data indicated a relationship between jasmonic acid signaling pathway and the functions of TwTGA1.

5.
Zhongguo Zhong Yao Za Zhi ; 44(18): 4053-4059, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31872745

RESUMO

Nanwuweizi( Schisandrae Sphenantherae Fructus) and Wuweizi( Schisandrae Chinensis Fructus) have long-term history of use as common traditional Chinese medicines since the Eastern Han Dynasty( AD.25-220 year).However their information are always confused in ancient literature because they were both used as " Wuweizi". Nanwuweizi and Wuweizi are faced with problems such as confused distribution of producing areas,unclear source plants and efficacy characteristics,which limit modern resource development and application. Based on ancient literatures of materia medica,this study conducted a systematic review from several aspects,i.e. the name,distribution of producing areas,source plants,efficacy characteristics and processing of the two medicines in ancient time. This study clarified five main aspects,as following,ancient production areas and corresponding modern distribution areas; source plants used for medicinal purposes in ancient time; application period and application scope; efficacy characteristics in clinical application;processing method. This study provides a reference for evaluating the quality and for their clinical application and reasonable development of Nanwuweizi and Wuweizi.


Assuntos
Medicamentos de Ervas Chinesas/história , Medicina Herbária/história , Materia Medica , Schisandra , China , História Antiga , Medicina Tradicional Chinesa , Fitoterapia , Plantas Medicinais
6.
Zhongguo Zhong Yao Za Zhi ; 44(20): 4405-4411, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31872625

RESUMO

The application of chemical fingerprint to evaluate the quality of traditional Chinese medicine has been widely accepted and used in many countries. However,only by analyzing the type and content of its chemical components to evaluate the quality of traditional Chinese medicines,the gold standard of quality evaluation by evaluating pharmacodynamic effects is ignored. The study of Chinese medicinal spectrum-effect relationships combining the chemical composition with the pharmacodynamic activity of traditional Chinese medicine,which can evaluate the quality of traditional Chinese medicine from more comprehensive and different angles,has been applied in many fields of traditional Chinese medicine research. This paper mainly summarizes the research methods of the Chinese medicinal spectrum-effect relationships and its application in the field of traditional Chinese medicine study,and provides reference for the research,development and application of the Chinese medicinal spectrum-effect relationships.


Assuntos
Medicamentos de Ervas Chinesas , Medicina Tradicional Chinesa , Registros , Projetos de Pesquisa
7.
Exp Ther Med ; 18(6): 4249-4258, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31772627

RESUMO

Non-small cell lung cancer (NSCLC) is the leading cause of lung cancer-associated mortality. Recent studies revealed that long non-coding (lnc)RNAs have crucial roles in human cancers. The present study was the first, to the best of our knowledge, to indicate that the lncRNA transducer of ERBB2, 1-antisense 1 (TOB1-AS1) acts as a tumor suppressor in NSCLC. Knockdown of TOB1-AS1 significantly induced NSCLC cell migration, invasion and proliferation. It was also demonstrated that the higher expression of TOB1-AS1 in NSCLC samples was associated with longer overall survival time. Furthermore, a TOB1-AS1-mediated competing endogenous RNA network in NSCLC was constructed, including Homo sapiens (hsa)-microRNA (miR)-27a-3p, hsa-miR-23a-3p, hsa-miR-23b-3p, hsa-miR-27b-3p, hsa-miR-23c, dynein cytoplasmic 2 light intermediate chain 1, E4F transcription factor 1, TSPY-like 4, component of oligomeric Golgi complex 7, inositol hexakisphosphate kinase 2 and deltex E3 ubiquitin ligase 3. Of note, dysregulation of targets of TOB1-AS1 was associated with the prognosis of NSCLC patients. The present study suggested that TOB1-AS1 may serve as a novel biomarker for NSCLC.

8.
Br J Neurosurg ; : 1-3, 2019 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-31564169

RESUMO

Sclerosing epithelioid fibrosarcoma (SEF) is a rare soft tissue tumour subtype, and those arising from the spine are even rarer. To the best of our knowledge, only 3 cases with the spine as the primary site of SEF have been previously reported. We report a 61-year-old female who presented with backache and bilateral leg numbness for 3 years, worsened over the last three months. Pathological fracture of the L1 vertebra was detected, and soft tissue density in the spinal canal and left vertebral body margin was also seen on contrast CT. She underwent tumour resection via a posterior approach, decompression, bone grafting, fusion, and internal fixation. Histology confirmed the diagnosis of SEF.

9.
Biometals ; 32(5): 757-769, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31363876

RESUMO

Ferritin is a ubiquitous iron storage protein which plays key role in regulating iron homeostasis and metabolism. In this paper, the ferritin heavy chain homologs (HCH) and light chain homologs (LCH) from Bombyx mori (BmFerHCH and BmFerLCH) were amplified through PCR and cloned into the expression vector pET-30a(+). The recombinant BmFerHCH and BmFerLCH expressed in Escherichia coli were in the form of insoluble inclusion bodies, indicating that the two proteins were not in their natural structural conformation. In order to obtain refolded ferritin in vitro, the inclusion bodies (BmFerHCH and/or BmFerLCH) were dissolved in denaturing buffer (100 mM Tris, 50 mM Glycine, 8 M urea, 5 mM DTT, pH 8.0) and then refolded in refolding buffer (100 mM Tris, 400 mM L-arginine, 0.2 mM PMSF, 0.5 mM DTT). The result showed that it was only when both BmFerHCH and BmFerLCH were present together in the denaturing buffer that refolding was successful and resulted in the formation of heteropolymers (H-L chain dimers) over homopolymers (H-H chain or L-L chain dimers). Moreover, the molecules (NaCl, Triton and glycerol) were found to enhance protein refolding. The optimum temperature, pH and ratios of BmFerHCH/BmFerLCH required for refolding were found to be 10 °C, pH 7, 1:1 or 1:2, respectively. Finally, the refolded ferritin had the ability to store iron, exhibited ferroxidase activity, and could withstand high temperatures and pH treatment, which is consistent with ferritin in other species.

10.
J Nat Prod ; 82(5): 1063-1071, 2019 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-31050424

RESUMO

Eight new cadinene-sesquiterpenes (1-8), one eudesmane-sesquiterpene (9), and three known compounds (10-13) were isolated from an endophytic fungus, Aspergillus flavus, which was isolated from a toxic medicinal plant, Tylophora ovata. Their structures were elucidated by interpretation of spectroscopic data, and absolute configurations determined according to the specific rotation and electron circular dichroism methods. Compounds 4-8, 11, and 12 exhibited latent hepatic protection effects at 10 µM, and compound 12 selectively inhibited the proliferation of MCF-7 breast cancer cells with an IC50 values of 2.6 µM.

11.
Zhongguo Zhong Yao Za Zhi ; 44(8): 1623-1634, 2019 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-31090327

RESUMO

Antithrombus is one of the effective methods to prevent and treat cardiovascular diseases. Based on the theory of traditional Chinese medicine,the author's previous research and relevant literature,it was found that the alkaloids in Houttuynia cordata has potential antithrombotic effect. However,the pharmacological substance basis and antithrombotic mechanism of H. cordata have not been clarified. In this study,molecular docking was used for virtual screening of antithrombotic alkaloids from H. cordata. Seventy alkaloids selected from H. cordata were screened in the docking ligand data-base with teen thrombosis targets with known crystal structures as the receptors. In addition,the small-molecule approved or to be approved drugs of targets from Drug Bank database were set as a positive reference with minimum score(S value) of each target's approved or to be approved drugs as threshold. The Dock module in Molecular Operating Environment(Version 2016) software was applied to screen the potential active compounds which their scores(S value) were lower than the minimum score of reference. At last the mechanism of antithrombotic effect was preliminarily revealed by compared the main active sites of the test alkaloids with original ligands and references. This study provided some useful information to development of antithrombus drugs.


Assuntos
Alcaloides/farmacologia , Fibrinolíticos/farmacologia , Houttuynia/química , Simulação de Acoplamento Molecular , Compostos Fitoquímicos/farmacologia
12.
Chin Med J (Engl) ; 132(10): 1208-1211, 2019 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-30882465

RESUMO

BACKGROUND: Blood purification (BP) is one of the most important rescue measures for patients with critical illness in the intensive care unit (ICU), especially for those with acute kidney injury. The purpose of this nationwide survey was to reveal the real world of current BP practice in different ICUs all over China. This study was designed to be a multi-center cross-sectional study. METHODS: All adult patients (over 18 years of age), who were admitted to ICU and required BP in 35 sub-centers across China were included during 30-day survey period in 2018. Demographic characteristics and clinical data were recorded including the timing of treatment initiation, indications, modality, relative contraindication, establishment of vascular access, selection of filter/membrane, settings, anti-coagulation, executive department, complication, intake, and output. DISCUSSION: This nationwide survey may contribute to reveal the real world of current BP practice in different ICUs all over China. TRIAL REGISTRATION: Chinese Clinical Trial Registry, ChiCTR-EOC-17013119; http://www.chictr.org.cn/showproj.aspx?proj=22487.


Assuntos
Hemofiltração/métodos , China , Estudos Transversais , Humanos , Unidades de Terapia Intensiva/estatística & dados numéricos , Modelos Teóricos
13.
ISA Trans ; 92: 134-144, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30826111

RESUMO

This paper deals with the stabilization problem for discrete-time linear system with unsymmetric saturation. To handle the structural properties of the unsymmetric saturation nonlinearity, the description of the transformation carries from the symmetric case to the unsymmetric case is employed. By using the parametric Lyapunov equation-based low gain feedback design approach, linear matrix inequality formulations for the state feedback and output feedback control design are given. The effectiveness of the proposed method is confirmed by numerical examples.

14.
Nat Prod Res ; 33(15): 2133-2138, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30822136

RESUMO

A new steroid lactone aspergilolide (1), and nine known compounds helvolic acid (2), verruculogen (3), tryprostatin B (4), 13-oxofumitremorgin B (5), fumitremorgin C (6), demethoxy fumitremorgin C (7), terezine D (8), aszonalenin (9), 12, 13-dihydroxy-fumitremorgin C (10) from cultures of the endophytic fungus Aspergillus sp. MBL1612. Their chemical structures were determined by a series of extensive spectroscopic methods. All of the compounds were isolated from this genus for the first time. The cytotoxicity against five human cancer cell lines of new compound were detected.


Assuntos
Aspergillus/metabolismo , Lactonas/metabolismo , Paeonia/microbiologia , Esteroides/biossíntese , Linhagem Celular Tumoral , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Lactonas/química , Lactonas/farmacologia , Análise Espectral/métodos , Esteroides/química , Esteroides/farmacologia
15.
PLoS One ; 13(10): e0205334, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30359377

RESUMO

The intrinsic factors (IF) influencing visual attention performance (VAP) might cause potential human errors, such as "error/mistake", "forgetting" and "omission". It is a key issue to develop a systematic assessment of IF in order to distinguish the levels of VAP. Motivated by the Stimulus-Response (S-R) model, we take an interactive cancellation test-Neuron Type Test (NTT)-to explore the IF and present the corresponding systematic assessment. The main contributions of this work include three elements: a) modeling the IF on account of attention span, attention stability, distribution-shift of attention with measurable parameters by combining the psychological and statistical concepts; b) proposing quantitative analysis methods for assessing the IF via its computational representation-intrinsic qualities (IQ)-in the sense of computational model; and c) clustering the IQ of air traffic control (ATC) students in the feature space of interest. The response sequences of participants collected with the NTT system are characterized by three parameters: Hurst exponent, normalized number of decisions (NNoD) and error rate of decisions (ERD). The K-means clustering is applied to partition the feature space constructed from practical data of VAP. For the distinguishable clusters, the statistical inference is utilized to refine the assessment of IF. Our comprehensive analysis shows that the IQ can be classified into four levels, i.e., excellent, good, moderate and unqualified, which has a potential application in selecting air traffic controllers subject to reducing the risk of the inadequacy of attention performances in aviation safety management.


Assuntos
Atenção/fisiologia , Aviação/normas , Tempo de Reação/fisiologia , Visão Ocular/fisiologia , Percepção Visual/fisiologia , Adulto , Algoritmos , Análise por Conglomerados , Humanos , Fator Intrínseco , Masculino , Modelos Teóricos
16.
Small ; 14(36): e1801095, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-30091845

RESUMO

Droplet microfluidics has been widely applied in functional microparticles fabricating, tissue engineering, and drug screening due to its high throughput and great controllability. However, most of the current droplet microfluidics are dependent on water-in-oil (W/O) systems, which involve organic reagents, thus limiting their broader biological applications. In this work, a new microfluidic strategy is described for controllable and high-throughput generation of monodispersed water-in-water (W/W) droplets. Solutions of polyethylene glycol and dextran are used as continuous and dispersed phases, respectively, without any organic reagents or surfactants. The size of W/W droplets can be precisely adjusted by changing the flow rate of dispersed and continuous phases and the valve switch cycle. In addition, uniform cell-laden microgels are fabricated by introducing the alginate component and rat pancreatic islet (ß-TC6) cell suspension to the dispersed phase. The encapsulated islet cells retain high viability and the function of insulin secretion after cultivation for 7 days. The high-throughput droplet microfluidic system with high biocompatibility is stable, controllable, and flexible, which can boost various chemical and biological applications, such as bio-oriented microparticles synthesizing, microcarriers fabricating, tissue engineering, etc.


Assuntos
Portadores de Fármacos/química , Microfluídica/métodos , Microesferas , Água/química , Alginatos/química , Animais , Cálcio/química , Linhagem Celular Tumoral , Dextranos/química , Secreção de Insulina , Peso Molecular , Polietilenoglicóis/química , Ratos , Reologia
17.
Biomed Pharmacother ; 106: 210-216, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29960167

RESUMO

Acute kidney injury (AKI) is a clinical syndrome that results in severe tubular damage with high morbidity and mortality. However, there is a lack of effective therapy strategies. Therefore, it is critical to develop effective drugs for AKI. Dexmedetomidine (DEX), a highly selective α2-adrenoreceptor agonist, has neuroprotective, anti-inflammatory and sympatholytic properties. The present study aimed to investigate the effect DEX on attenuating the inflammatory reaction and apoptosis in the kidney tissues of septic mice and to explore its underlying mechanisms. Sepsis-induced AKI mice models were generated via intraperitoneal injection of lipopolysaccaride (LPS). DEX reduced LPS-induced local inflammation and tubular apoptosis, which was aggravated in the pathogenesis of renal dysfunction. Reverse transcription-quantitative polymerase chain reaction and western blot analysis results revealed that the expression of pro-apoptotic genes and inflammatory factors were markedly reduced by DEX pretreatment. Furthermore, the protective role of DEX was markedly inhibited by the α7 nicotinic acetylcholine receptor (nAChR) antagonist α-bungarotoxin. These findings provided novel evidence for the anti-apoptotic and anti-inflammatory effects of DEX in LPS-induced AKI mice through an α7 nAChR-dependent signaling pathway.


Assuntos
Lesão Renal Aguda/prevenção & controle , Anti-Inflamatórios/farmacologia , Apoptose/efeitos dos fármacos , Dexmedetomidina/farmacologia , Túbulos Renais/efeitos dos fármacos , Lipopolissacarídeos , Sepse/tratamento farmacológico , Receptor Nicotínico de Acetilcolina alfa7/metabolismo , Lesão Renal Aguda/induzido quimicamente , Lesão Renal Aguda/metabolismo , Lesão Renal Aguda/patologia , Animais , Proteínas Reguladoras de Apoptose/genética , Proteínas Reguladoras de Apoptose/metabolismo , Citoproteção , Modelos Animais de Doenças , Mediadores da Inflamação/metabolismo , Túbulos Renais/metabolismo , Túbulos Renais/patologia , Masculino , Camundongos Endogâmicos C57BL , Sepse/induzido quimicamente , Sepse/metabolismo , Transdução de Sinais/efeitos dos fármacos , Fatores de Tempo
18.
Molecules ; 23(6)2018 May 26.
Artigo em Inglês | MEDLINE | ID: mdl-29861462

RESUMO

A phytochemical investigation of the stems of Kadsura interior has led to an isolation of four new lignans, named kadsutherin E⁻H (1⁻4), together with two known lignans (5⁻6). The structures of the four new compounds were established on the basis of comprehensive spectroscopic analyses. Compounds 1⁻6 exhibited inhibition against adenosine diphosphate (ADP) induced platelet aggregation. Among the isolated compounds, kadsutherin F (2) showed the strongest anti-platelet aggregation activity with an inhibition of 49.47%.


Assuntos
Kadsura/química , Lignanas/química , Lignanas/farmacologia , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Dicroísmo Circular , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Compostos Fitoquímicos/química , Compostos Fitoquímicos/farmacologia , Inibidores da Agregação de Plaquetas/química , Inibidores da Agregação de Plaquetas/farmacologia
19.
Med Sci Monit ; 24: 2489-2496, 2018 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-29684003

RESUMO

BACKGROUND The formation of new blood vessels, known as angiogenesis, is critical for recovery from ischemic heart disease, and estrogen is considered an important factor in this process. Here, we investigated the effects of 17ß-estradiol (17ß-E2) on proliferation and migration of cardiac microvascular endothelial cells (CMECs) in vitro. MATERIAL AND METHODS Rat CMECs were isolated and cultured with 17ß-E2 (0.001-1 µmol/l) in the absence or presence of the estrogen antagonist tamoxifen. Then, the expression level of estrogen receptor alpha was evaluated by using immunofluorescence assay, RT-PCR, and Western blot. Cell proliferation was detected by methyl thiazolyl tetrazolium analysis and the cell migration was verified by a scraping assay and quantified by a Transwell chamber assay. CMEC differentiation was examined using a tube formation assay. Vascular endothelial growth factor (VEGF) secretion was detected by enzyme-linked immunosorbent assay. RESULTS CMECs exhibited homogenous, polygonal, exhibited contact inhibition, and had characteristically ovoid nuclei with 1 or 2 nucleoli, and the cytoplasm exhibited red fluorescence after staining for von Willebrand factor. 17ß-E2 treatment upregulated estrogen receptor alpha expression in CMECs. 17ß-E2 treatment significantly promoted the proliferation, migration, tubular structure formation, and VEGF secretion in CMECs. The maximal proliferation occurred in the presence of 0.01 µmol/l 17ß-E2. Furthermore, estrogen and VEGF were found to synergistically stimulate angiogenesis. CONCLUSIONS Our data show that 17ß-E2 promotes angiogenesis in vitro and suggests that estrogen treatment as a novel therapeutic modality in the management of arterial insufficiency.


Assuntos
Células Endoteliais/efeitos dos fármacos , Estradiol/farmacologia , Indutores da Angiogênese , Animais , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Endoteliais/citologia , Células Endoteliais/metabolismo , Endotélio Vascular/citologia , Endotélio Vascular/efeitos dos fármacos , Endotélio Vascular/metabolismo , Coração/efeitos dos fármacos , Masculino , Isquemia Miocárdica/metabolismo , Isquemia Miocárdica/patologia , Miocárdio/citologia , Miocárdio/patologia , Neovascularização Patológica/tratamento farmacológico , Neovascularização Patológica/metabolismo , Cultura Primária de Células , Ratos , Ratos Sprague-Dawley , Transdução de Sinais/efeitos dos fármacos , Regulação para Cima/efeitos dos fármacos , Fator A de Crescimento do Endotélio Vascular/metabolismo
20.
J Phys Chem A ; 122(16): 4085-4091, 2018 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-29641202

RESUMO

The structural and electronic properties of Pu2O n ( n = 1-8) molecules have been systematically studied within the screened hybrid density functional theory. On the basis of the calculations, plutonium and oxygen generally prefers forming Pu-O and Pu-O-Pu bonds over to Pu-Pu or O-O bonds. In the ground-state geometries, we find that the highest oxidation state for plutonium atoms is Pu(VI). Through fragmentation studies, we find that the Pu2O and Pu2O2 molecules are energetically stable among all studied compounds. When fixing the Fermi level to be energy zero, the Pu-5 f states always distribute at the two sides of the Fermi level. With increasing the number of oxygen atoms and the oxidation state of plutonium, the Pu-6d states shift down from above to below the Fermi level.

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