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1.
Horm Metab Res ; 54(2): 113-118, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-35130572

RESUMO

The function of miR-551b has been widely reported in various human cancers, and its dysregulation in papillary thyroid cancer (PTC) has also been disclosed, implying its potential regulator role in PTC. The aim of the study was to evaluate the function of miR-551b in PTC development and its potential mechanism. miR-551b was evaluated in PTC tissues and cells by RT-qPCR and associated with the clinicopathological features of patients. The biological effect of miR-551b on cellular processes of PTC was assessed with the CCK8 proliferation assay and the Transwell migration and invasion assay. The potential molecular mechanism was estimated with the dual-luciferase reporter assay. miR-551b was significantly upregulated in PTC, which showed a close relationship with the malignancy and development of PTC patients. miR-551b served as a prognostic biomarker negatively related to patients' survival together with the TNM stage. The overexpression of miR-551b exerted promoted effect on the development-related cellular processes of PTC, which was reversed by the overexpression of ERBB4. In conclusion, miR-551b could predict the poor prognosis of PTC patients and serve as a tumor promoter via suppressing ERBB4.


Assuntos
MicroRNAs , Neoplasias da Glândula Tireoide , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Regulação Neoplásica da Expressão Gênica , Humanos , MicroRNAs/genética , Prognóstico , Receptor ErbB-4/genética , Câncer Papilífero da Tireoide/genética , Neoplasias da Glândula Tireoide/genética , Neoplasias da Glândula Tireoide/patologia
2.
Artif Intell Med ; 128: 102308, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35534146

RESUMO

Left ventricular assist device (LVAD) is an effective method to treat ventricular failure. According to the physiological conditions of different patients, the device adaptively adjusts its rotation speed to change LVAD output. In this study, a physiological control system for LVAD based on deep reinforcement learning (DRL) is proposed. The system estimates the amount of blood required by LVAD based on a Starling-like method. The DRL controller regulates LVAD to adjust the speed and quickly approach the target value. The changes of vascular resistance, myocardial contractility, and the transition from rest to exercise were simulated, and the single factor and mixed factor experiments were carried out to compare the effects of DRL controller and proportional integral derivative (PID) controller, which controls the system according to the difference between measured variables and expected values. Two metrics are used to illustrate the regulation effect: the sum of absolute error (SAE) and the response time of the two controllers, where SAE is the difference between the estimated required pumped blood flow LVADQe and the actual measured blood flow LVADQm. The experimental result shows that the SAE of the DRL controller is 47.6% of that of the PID controller, and the response time of the DRL controller is 38.6% of that of the PID controller. This study demonstrates that the LVAD based on the DRL controller can respond more quickly and more effectively to the different physiological needs of a variety of patients than a PID controller.


Assuntos
Insuficiência Cardíaca , Coração Auxiliar , Algoritmos , Simulação por Computador , Insuficiência Cardíaca/terapia , Humanos , Modelos Cardiovasculares
3.
Water Res ; 219: 118529, 2022 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-35569277

RESUMO

A system of Cu2+/calcite/PDS was constructed to degrade sulfadiazine (SDZ). Different from the traditional Cu-mediated activation, a low concentration of Cu2+ that met drinking water standards (≤ 1 mg/L) transformed into Cu(Ⅱ) solid in the presence of calcite, and then enhanced the degradation of SDZ via PDS activation over a pH range from 3 to 9. According to scavenger and chemical probe experiments, Cu(Ⅲ), rather than radicals (hydroxyl radicals and sulfate radicals) and singlet oxygen, was the predominant reactive species, which was responsible for the degradation of SDZ. Based on the results of XRD, ATR-FTIR, and CV curves et al., CuCO3 was the main complex with high reactivity for PDS activation to form Cu(Ⅲ). Moreover, detailed degradation pathways of sulfadiazine were proposed according to the UPLC-ESI-MS/MS and their toxicity was predicted by ECOSAR. Besides, the real water matrix would not seriously affect the degradation of SDZ in the Cu2+/calcite/PDS system. In summary, this study reveals a new insight into the synergistic effect of Cu2+ and calcite on the SDZ degradation, and promotes an understanding of the environmental benefits of natural calcite.

4.
Front Bioeng Biotechnol ; 10: 893337, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35600894

RESUMO

Background: Femoral neck fracture (FNF) is the most serious bone disease in the elderly population. The multiscale mechanical response is a key to predicting the strength of the femoral neck, assessing the risk of FNF, and exploring the role of mechanosensation and mechanotransmission in bone remodeling, especially in the context of aging bone. Methods: Multiscale finite element (FE) models of the proximal femur for both young and elderly people were developed. The models included organ scale (proximal femur), tissue scale (cortical bone), tissue element scale (osteon), and cell scale [osteocyte lacuna-canalicular network (LCN) and extracellular matrix (ECM), OLCEM]. The mechanical responses of cortical bone and osteocytes in the mid-femoral neck and the differences in mechanical responses between these two scales were investigated. Results: The mechanical responses of cortical bone and osteocyte showed significant differences between the elderly and the young. The minimum principal strains and mean SEDs of cortical bone in the elderly were 2.067-4.708 times and 3.093-14.385 times of the values in the young, respectively; the minimum principal strains and mean SEDs of osteocyte in the elderly were 1.497-3.246 times and 3.044-12 times of the values in the young, respectively; the amplification factors of minimum principal strain in the inferior (Inf), anterior (Ant), and posterior (Post) quadrants in the young were 1.241-1.804 times of the values in the elderly, but the amplification factor of minimum principal strain in the superior (Sup) quadrant was 87.4% of the value in the elderly; the amplification factors of mean SED in the young were 1.124-9.637 times of the values in the elderly. Conclusion: The mass and bone mineral density (BMD) of cortical bone in the femoral neck is closely related to the mechanical response of osteocytes, which provides a new idea for improving cortical bone quality. Perhaps cortical bone quality could be improved by stimulating osteocytes. Quadrantal differences of bone quality in the mid-femoral neck should be considered to improve fracture risk prediction in the future.

5.
NanoImpact ; 25: 100368, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35559876

RESUMO

The increasing application of zinc oxide nanoparticles (ZnO NPs) in consumer products has raised concerns about the potential health risks in human. It is crucial to understand the toxicokinetic information of ZnO NPs, especially the differences between NPs and non-nano form material. This study investigated the toxicokinetic profile of ZnO NPs and food grade bulk-sized ZnO in rats after single or repeated oral dosages. For single oral administration of ZnO suspensions at 350 mg/kgbw, the Zn content in blood and tissues showed no elevation, the majority of ZnO particles were eliminated via feces within 48 h. For repeated oral exposure to ZnO suspensions at 350 mg/kgbw or ZnSO4 solution at 700 mg/kgbw for 90 days, elevated Zn levels were observed in liver, kidney, and bone in all three treatment groups, the Zn level recovered to normal level in liver and kidney, but not in bone, after a recovery period. ZnO NPs and bulk-sized ZnO showed similarity in toxicokinetics in rats, regardless of exposure duration or gender. ZnO particles shared a similar biodistribution profile with ZnSO4, and were likely to be absorbed mostly in ionic forms.

6.
Comput Intell Neurosci ; 2022: 4192367, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35463224

RESUMO

Pedestrian trajectory prediction is an essential but challenging task. Social interactions between pedestrians have an immense impact on trajectories. A better way to model social interactions generally achieves a more accurate trajectory prediction. To comprehensively model the interactions between pedestrians, we propose a multilevel dynamic spatiotemporal digraph convolutional network (MDST-DGCN). It consists of three parts: a motion encoder to capture the pedestrians' specific motion features, a multilevel dynamic spatiotemporal directed graph encoder (MDST-DGEN) to capture the social interaction features of multiple levels and adaptively fuse them, and a motion decoder to produce the future trajectories. Experimental results on public datasets demonstrate that our model achieves state-of-the-art results in both long-term and short-term predictions for both high-density and low-density crowds.


Assuntos
Pedestres , Previsões , Humanos , Movimento (Física)
8.
Oncoimmunology ; 11(1): 2057399, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35371619

RESUMO

Microbial dysbiosis plays an important role in the development of intestinal diseases. Recent studies suggest a link between intestinal bacteria and mammary cancer. Here, we report that female ApcMin/+ mice infected with Helicobacter hepaticus exhibited an increased mammary and small/large intestine tumor burden compared with uninfected littermates. H. hepaticus DNA was detected in small/large intestine, mammary tumors, and adjacent lymph nodes, suggesting a migration pathway. CD11b+Gr1+ myeloid-derived suppressor cells (MDSCs) infiltrated and expressed high levels of Wnts, likely enhancing tumorigenesis through activation of Wnt/ß-catenin pathway. Our previous studies indicated that histidine decarboxylase (Hdc) marks a population of myeloid-biased hematopoietic stem cells and granulocytic MDSCs. Cytokines/chemokines secreted by IL-17-expressing mast cells and tumor tissues promoted Hdc+ MDSCs expansion and trafficking toward mammary tumors. Adoptive transfer of MDSCs isolated from H. hepaticus-infected mice increased MDSCs frequencies in peripheral blood, mesenteric lymph nodes, mammary gland, and lymph nodes in recipient ApcMin/+ mice. The adoptive transfer of H. hepaticus primed MDSCs also increased the size and number of mammary tumors. Our results demonstrate that H. hepaticus can translocate from the intestine to mammary tissues to promote mammary tumorigenesis with MDSCs. Targeting bacteria and MDSCs may be useful for the prevention and therapy of extraintestinal cancers. Abbreviations: Helicobacter hepaticus, Hh; myeloid-derived suppressor cell, MDSC; histidine decarboxylase, Hdc; Breast cancer, BC; T regulatory, TR; inflammatory bowel disease, IBD; fluorescence in situ hybridization, FISH; myeloid-biased hematopoietic stem cells, MB-HSCs; granulocytic MDSCs, PMN-MDSCs; Lipopolysaccharide, LPS; Toll-like receptors, TLRs; Mast cells, MCs; Granulocyte-macrophage colony-stimulating factor, GM-CSF; epithelial-mesenchymal transition, EMT; Intestinal epithelial cells, IECs.


Assuntos
Células Supressoras Mieloides , Animais , Transformação Celular Neoplásica/metabolismo , Feminino , Helicobacter hepaticus , Hibridização in Situ Fluorescente , Camundongos , Camundongos Endogâmicos BALB C , Células Supressoras Mieloides/metabolismo
9.
Med Sci Monit ; 28: e934703, 2022 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-35304433

RESUMO

BACKGROUND This retrospective study aimed to compare the effects of methylprednisolone and dexamethasone in 35 patients with anaphylactic shock due to iodinated contrast media (ICM) during cardiac catheterization. MATERIAL AND METHODS Thirty-five patients diagnosed with ICM-related anaphylactic shock were enrolled. Nineteen patients received methylprednisolone, while 16 patients received dexamethasone. RESULTS The mean age of patients was 59.8 years in the methylprednisolone group and 57.2 years in the dexamethasone group (P=0.502). Most patients were male (82.9% [26/35]). The groups were matched for cardiac function, kidney function, comorbidities, prior contrast, previous radiocontrast media reactions, and allergic tendencies (all P>0.05). The severity of coronary artery disease, assessed using the SYNTAX II score, was similar between groups (P=0.620). Six patients died from ICM-induced anaphylactic shock. There was no significant difference in mortality between the 2 groups (P=1.000). CONCLUSIONS There was no significant difference in patient mortality from anaphylactic shock associated with ICM during cardiac catheterization between the methylprednisolone and dexamethasone groups.


Assuntos
Anafilaxia/induzido quimicamente , Anafilaxia/tratamento farmacológico , Cateterismo Cardíaco , Meios de Contraste/efeitos adversos , Dexametasona/farmacologia , Compostos de Iodo/efeitos adversos , Metilprednisolona/farmacologia , Anti-Inflamatórios/farmacologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos
10.
Chemosphere ; 297: 134131, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35257708

RESUMO

In this work, the effect of Co substitution in the Fe1-xS (CSP) on the activation of H2O2 to degrade tetracycline (TC) is investigated. A series of CSP samples with different Co content are synthesized via a high-temperature sulfidation method and characterized by XRD, XPS, SEM, and electrochemical analysis. The result showed that low Co content (≤1%) promotes the catalytic activity of Fe1-xS, while excessive Co (1%﹤x ≤ 3%) inhibits its catalytic activity. The investigation of Behnajady-Modirshahla-Ghanbery kinetic model (BMG) showed that the maximum initial degradation rate of TC over 1.0% CSP/H2O2 was 1.6 times than that of in CSP/H2O2 system. The Box-Behnken with Response Surface Methodology was employed to verify optimum condition for TC degradation. The quenching experiments and ESR determined that ·OH, ·O2- and 1O2 were involved in TC degradation with the treatment of 1.0% CSP/H2O2 system. Electrochemical analysis, ·OH quantification, and metal ion concentrations measure reveal that Co substitution accelerates electron transfer efficiency and Fe2+ regeneration. Furthermore, nine intermediates are identified and the possible degradation pathway of TC is proposed. The unique effect of Co provides novel insight and efficient strategies for improving the reactivity of iron sulfide.


Assuntos
Peróxido de Hidrogênio , Tetraciclina , Antibacterianos , Catálise , Transporte de Elétrons , Peróxido de Hidrogênio/química
11.
Ann Transl Med ; 10(4): 204, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-35280397

RESUMO

Background: Periodontitis is a highly prevalent dental disease which is associated with diabetes and is challenging to cure in diabetic patients. However, the mechanism of comorbid diabetes and periodontitis is still unclear. This study aimed to uncover the role of endoplasmic reticulum (ER) stress in high glucose-associated periodontitis. Methods: Periodontal tissues were obtained from diabetic patients with periodontitis, periodontitis patients without systemic disease, and healthy teeth. The expressions of ER stress-related factors GRP78, ATF6, PERK and XBP1 were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and immumohistochemical staining. Periodontal ligament stem cells (PDLSCs) from three states of periodontal tissues were isolated and cultured as diabetic PDLSCs (dPDLSCs), inflamed PDLSCs (iPDLSCs) and healthy PDLSCs (hPDLSCs), and the cell stemness was assayed. Different concentrations (8, 11, and 25 mmol/L) of D-glucose were used on hPDLSCs to simulate high glucose microenvironment. The changes of osteogenic ability of PDLSCs were observed, and the expressions of ER stress-related factors in different time point (6, 12, 24, and 72 h) were detected. Finally, GRP78 shRNA lentivirus was used to block ER stress on PDLSCs in the 25 mmol/L D-glucose microenvironment, and the osteogenic ability of PDLSCs was observed. Results: The results showed that the expressions of GRP78, ATF6, PERK, and XBP1 were highest in the diabetic periodontitis group and lowest in the healthy periodontal tissue group (P<0.05). The clone formation, osteogenic and lipogenic differentiation abilities were lowest in dPDLSCs and highest in hPDLSCs. With the increase of glucose concentration, the osteogensis ability of PDLSCs decreased. After 6 hours of stimulation with D-glucose 25 mmol/L, the ER stress pathways in PDLSCs were effectively activated, and the peak value was reached at 12 hours. The decrease in the osteogensis ability of PDLSCs in a high glucose microenvironment reversed when ER stress was blocked. Conclusions: The osteogenic differentiation ability of PDLSCs cells is inhibited in a high glucose microenvironment, and this effect is realized by ER stress activation. Blocking ER stress can partially restore the reduced osteogenic ability of PDLSCs. These results suggest that high glucose inhibits the osteogenic differentiation ability of PDLSCs by activating ER stress, which ultimately exacerbates periodontitis.

12.
Catheter Cardiovasc Interv ; 99 Suppl 1: 1418-1423, 2022 05.
Artigo em Inglês | MEDLINE | ID: mdl-35120269

RESUMO

OBJECTIVES: We aimed to evaluate the efficacy of a protective ballooning technique in preventing side branch (SB) occlusion and to assess the long-term clinical outcomes for coronary nonleft main true bifurcation lesions. BACKGROUND: SB occlusion is a major complication associated with percutaneous coronary intervention (PCI) for coronary bifurcation lesions. METHODS: Patients were consecutively enrolled and randomly assigned to protective ballooning technique or jailed wire technique group. Periprocedural and long-term clinical outcomes were compared. RESULTS: Patients in the protective ballooning technique (n = 173) and jailed wire technique (n = 167) groups were followed up for 12 months. SB occlusion occurred in one patient (0.6%) and nine patients (5.4%) in each group, respectively. The proportion of thrombolysis in myocardial infarction (TIMI) flow grade 3 of the SB was higher in the protective ballooning technique group (98.8% vs. 95.2%, p < 0.05). SB rewiring was necessary in one patient in the protective ballooning technique group (0.6%) with provisional stenting, significantly lower than that in the jailed wire technique group (seven patients, 4.2%; p = 0.03). Periprocedural myocardial infarction occurred in three (1.73%) and six (3.59%) patients in the protective ballooning technique and jailed wire technique groups without significant difference, respectively. Major adverse cardiovascular events at 12 months were similar in both groups. CONCLUSIONS: Protective ballooning technique is effective for the prevention of SB occlusion in nonleft main true bifurcation lesions and had favorable long-term outcomes at the 12-month follow-up.


Assuntos
Angioplastia Coronária com Balão , Doença da Artéria Coronariana , Infarto do Miocárdio , Intervenção Coronária Percutânea , Angioplastia Coronária com Balão/efeitos adversos , Doença da Artéria Coronariana/complicações , Doença da Artéria Coronariana/diagnóstico por imagem , Doença da Artéria Coronariana/terapia , Humanos , Infarto do Miocárdio/etiologia , Infarto do Miocárdio/prevenção & controle , Intervenção Coronária Percutânea/efeitos adversos , Resultado do Tratamento
13.
Int J Mol Med ; 49(4)2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35119082

RESUMO

Following the publication of the above article, the authors have realized that they inadvertently included incorrect data panels in Fig. 7 on p. 323; specifically, while preparing the figure, the panels denoting the 'CD31 DEXs­miRNA­194­3p inhibitor' and 'VEGF­DEXs­blank' panels were imported incorrectly in Fig. 7C. The revised version of Fig. 7, containing the correct data for the abovementioned panels, is shown below. Note that the errors made during the assembly of this figure did not affect the overall conclusions reported in the paper. All the authors agree with the publication of this corrigendum, and are grateful to the Editor of International Journal of Molecular Medicine for allowing them the opportunity to publish this. They also apologize to the readership for any inconvenience caused. [the original article was published in International Journal of Molecular Medicine 47: 315­325, 2021; DOI: 10.3892/ijmm.2020.4776].

14.
J Biol Eng ; 16(1): 2, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34986861

RESUMO

BACKGROUND: Although long non-coding RNA (lncRNA) NCK1-AS1 plays important roles in human cancer, its function in atherosclerosis (AS) remains unclear. METHOD: The expression of NCK1-AS1 in AS blood samples was detected by qRT-PCR. Oxidized low-density lipoprotein (ox-LDL) was used to construct the AS cell model, and quantitative real-time polymerase chain reaction (qRT-PCR) assay was used to evaluate NCK1-AS1 level. Cell phenotypes including proliferation and apoptosis were assessed by Cell Counting Kit-8 (CCK-8) assay and flow cytometer, respectively. The malondialdehyde level was measured to evaluate oxidative stress. The expression of apoptosis-related proteins was evaluated by western blot. The expression of inflammatory cytokines (IL-1ß, IL-6 and TNK-α) was measured by qRT-PCR and ELISA assays. The relationship among NCK1-AS1, miR-1197 and COX10 was determined by bioinformatic analysis and luciferase reporter assay. RESULTS: NCK1-AS1 was significantly upregulated in AS blood samples and ox-LDL stimulated vascular smooth muscle cells (VSMCs). Knockdown of NCK1-AS1 increased cell viability, reduced cell apoptosis and MDA level, and also inhibited the expression of inflammatory cytokines (IL-1ß, IL-6 and TNK-α) in ox-LDL stimulated VSMCs. NCK1-AS1 could positively regulate COX10 expression by directly sponging miR-1197. Moreover, co-transfection of sh-NCK1-AS1 and miR-1197 inhibitor, or co-transfection of sh-NCK1-AS1 and pc-COX10 (COX10 overexpressing plasmid) obviously reduced cell viability, promoted cell apoptosis, and increased MDA level in VSMCs followed by ox-LDL treatment for 24 h compared to that in sh-NCK1-AS1 transfected VSMCs. CONCLUSION: Our study revealed that knockdown of NCK1-AS1 attenuated the development of AS by regulating miR-1197/COX10 axis, suggesting that this lncRNA might be a potential therapeutic target for AS.

15.
Environ Sci Pollut Res Int ; 29(25): 38006-38016, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35067884

RESUMO

Naturally occurring Mn-rich limonite mainly composed of goethite and manganese oxides was used to remove tetracycline (TC) from the aqueous solution. The effects of dosage, initial solution pH, temperature, and coexisting anions on TC removal were investigated. Results showed that 95% of TC (30.0 mg·L-1) was removed in a wide pH range of 3.0-9.0 by limonite with high specific surface area (145.0 m2·g-1) and mesoporous structure. The presence of Cl-, NO3-, and SO42- in the studied concentration range did not influence TC removal efficiency significantly, while PO43- inhibited the adsorption of TC over limonite due to the competition with TC for active sites. Integrated with the FT-IR analysis, electrostatic interaction and complexation were proved to be the adsorption mechanisms of TC by limonite. The quenching experiments and ESR analysis revealed that singlet oxygen (1O2) also was involved in TC degradation. In addition, limonite displayed an efficient recycling performance and stability after four cycles. This study revealed that the Mn-rich limonite was a promising adsorbent for TC removal from aqueous solutions and promoted the application of natural mineral material in the environmental field.

16.
Sci Adv ; 8(1): eabj9513, 2022 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-34995107

RESUMO

We pursued the hypothesis that specific glycans can be used to distinguish breast cancer stem cells (CSCs) and influence their function. Comparison of CSCs and non-CSCs from multiple breast cancer models revealed that CSCs are distinguished by expression of α2,3 sialylated core2 O-linked glycans. We identified a lectin, SLBR-N, which binds to O-linked α2,3 sialic acids, that was able to enrich for CSCs in vitro and in vivo. This O-glycan is expressed on CD44 and promotes its interaction with hyaluronic acid, facilitating CD44 signaling and CSC properties. In contrast, FUT3, which contributes to sialyl Lewis X (sLeX) production, is preferentially expressed in the non-CSC population, and it antagonizes CSC function. Collectively, our data indicate that SLBR-N can be more efficient at enriching for CSCs than CD44 itself because its use avoids the issues of CD44 splicing and glycan status. These data also reveal how differential glycosylation influences CSC fate.

17.
Environ Sci Technol ; 56(2): 823-834, 2022 01 18.
Artigo em Inglês | MEDLINE | ID: mdl-35038870

RESUMO

Despite the biogeochemical importance of phosphate fate and transport in aquatic environments, little is known about how competition with other common aqueous oxyanions affects its retention by mineral surfaces. Here, we examined the competitive uptake of phosphate and silicate on goethite over a wide pH range, using batch measurements supported by DFT calculations. The results show selective adsorption of phosphate at pH < 4 and silicate at pH > 10 with little to no competitive effect. However, between 4 < pH < 10, the total phosphate and silicate loading was found to be almost equal to that of silicate loading from single-component solution, revealing a proportionate competition for surface site types and a competitive effect controlling their mutual retention. DFT-calculated adsorption energies and charge density redistributions for various surface complexes on different charged (101) and (210) facets are consistent with the trends observed in batch measurements, suggesting that the observed behavior reflects the primary controlling influence of goethite surface chemistry at the molecular scale. An important implication is that at the circumneutral pH in most environmental systems, where iron oxyhydroxides comprise much of the reactive interfacial area, unbound phosphate concentrations may be strongly controlled by dissolved silicate concentration, and vice versa.


Assuntos
Compostos de Ferro , Fosfatos , Adsorção , Teoria da Densidade Funcional , Concentração de Íons de Hidrogênio , Compostos de Ferro/química , Minerais/química , Fosfatos/química , Silicatos
18.
Bioprocess Biosyst Eng ; 45(3): 515-525, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35059820

RESUMO

The phenomenon that the anaerobic system is inhibited by acid has always been a bottleneck hindering the application of anaerobic digestion (AD) technology. We tried to introduce electrolysis into AD to improve the acidification resistance, and eventually the productivity of the energy. In a batch fermentation device, the ability of electrochemical anaerobic digestion (EAD) to resist acidification was evaluated in current intensity, electrode potential, AC impedance, microbial community, pH value, and volatile fatty acids (VFAs). The results showed that the average concentration of VFAs in EAD was 32.9% lower than that in AD, the energy efficiency of EAD is 53.25% higher than AD, indicating that EAD has stronger anti-acidification ability and energy conversion efficiency than AD. When the EAD reaches a steady state, the current intensity fluctuates in the range of 7-12 mA, the electrode potential difference is maintained at 600 ± 5 mV, and the internal resistance decreases from 3333.3 ± 16Ω at startup to 68.9 ± 1.4Ω at the steady state, indicating that the EAD has stronger resistance to acidification may be due to the degradation of some VFAs on the electrode surface. Furthermore, the 16S rRNA sequencing analysis showed that the dominant electricity-producing bacteria on EAD anode surface were Clostridium, Hydrogenophaga and Trichloromonas, with a relative abundance of 40.32%, while the relative abundance of electrogenic bacteria in AD bulk solution and EAD bulk solution were about 1/2 and 1/4 that of EAD anode film, suggesting that the electricity-producing bacteria on the electrode surface play an important role in the degradation of VFAs.


Assuntos
Reatores Biológicos , Ácidos Graxos Voláteis , Anaerobiose , Reatores Biológicos/microbiologia , Eletrólise , Concentração de Íons de Hidrogênio , RNA Ribossômico 16S/genética , RNA Ribossômico 16S/metabolismo , Esgotos
19.
Regul Toxicol Pharmacol ; 128: 105091, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34863905

RESUMO

The present study aimed to evaluate the subchronic toxicity of feeding with phytase-transgenic maize line 11TPY050 in Sprague-Dawley (SD) rats. Rats (n = 10/sex/group) were fed with 12.5%, 25% or 50% (w/w) transgenic maize diet, 12.5%, 25% or 50% (w/w) non-transgenic isoline OSL940 maize diet, or 50% (w/w) commercially available Zhengdan958 maize diet for 90 days. Daily clinical observations and weekly measurements of body weights and food consumption were conducted. Blood samples were collected on day 46 and day 91 for hematology and clinical chemistry evaluations. At the end of the study, macroscopic and microscopic examinations were performed. No effects on body weight and food consumption were observed. The results of hematology, clinical chemistry, and absolute and relative organ weights in the transgenic maize group were comparable to those in the parental maize group. Several statistical differences were not dose-related and were not considered to be biologically significant. Furthermore, the terminal necropsy and histopathological examination showed no treatment-related changes among the groups. The results from the present 90-day feeding study of phytase-transgenic maize 11TPY050 indicated no unexpected adverse effects in SD rats. The phytase transgenic maize 11TPY050 has substantial equivalence with non-transgenic maize.


Assuntos
6-Fitase/administração & dosagem , Plantas Geneticamente Modificadas/toxicidade , Zea mays/genética , Animais , Peso Corporal/efeitos dos fármacos , Relação Dose-Resposta a Droga , Comportamento Alimentar/efeitos dos fármacos , Feminino , Testes Hematológicos , Masculino , Tamanho do Órgão/efeitos dos fármacos , Plantas Geneticamente Modificadas/enzimologia , Ratos , Ratos Sprague-Dawley
20.
Bioengineered ; 13(1): 1276-1287, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34738865

RESUMO

Long non-coding RNAs (lncRNAs) are closely associated with the development of lung adenocarcinoma (LADC). The present study focused on the role of LINC00960 in LADC. miRNA and mRNA expression levels were detected using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Cellular functions were evaluated by MTT, colony formation, and Transwell assays, respectively. LINC00960 Luciferase and RNA pull-down assays were performed to clarify the interaction between miR-124a and LINC00960 or Recombinant Sphingosine Kinase 1 (SphK1). We observed that LINC00960 was overexpressed in LADC tumor tissues and cell lines. LINC00960 knockdown suppressed the proliferation, migration, and invasion of LADC cells. Moreover, LINC00960 sponged miR-124a to inhibit the SphK1/S1P pathway in LADC cells. LINC00960 knockdown markedly reduced the rate of tumor growth. The luciferase reporter assay results demonstrated an interaction between miR-124a and LINC00960 or SphK1. This interaction was confirmed using the RNA pull-down assay. In addition, miR-124a downregulation or SphK1 upregulation reversed the inhibitory effects of LINC00960 knockdown on cellular functions of LADC cells, suggesting that LINC00960 may be a potential therapeutic biomarker for LADC via the miR-124a/SphK1 axis. Accordingly, LINC00960 may be a potential therapeutic biomarker for LADC.


Assuntos
Adenocarcinoma de Pulmão/patologia , Neoplasias Pulmonares/patologia , MicroRNAs/genética , Fosfotransferases (Aceptor do Grupo Álcool)/genética , RNA Longo não Codificante/genética , Regulação para Cima , Células A549 , Adenocarcinoma de Pulmão/genética , Animais , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Feminino , Regulação Neoplásica da Expressão Gênica , Técnicas de Silenciamento de Genes , Humanos , Neoplasias Pulmonares/genética , Masculino , Camundongos , Metástase Neoplásica , Transplante de Neoplasias , Fosfotransferases (Aceptor do Grupo Álcool)/metabolismo
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