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1.
Biophys Chem ; 292: 106916, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36343393

RESUMO

Human respiratory syncytial virus (RSV) is a primary cause of lower respiratory tract infections and hospital visits during infancy and childhood. The RSV phosphoprotein (P) is a major polymerase cofactor that interacts with nucleoprotein (N) to promote the recognition of ribonucleoprotein complex (RNP) by viral RNA polymerase. The binding pocket of N protein is chemically diverse, in or around which a number of aromatic and charged amino acid residues are observed. Previously, a nonapeptide segment (P peptide, 233DNDLSLEDF241) representing the C-terminal tail of P protein was identified to mediate the N-P interaction with a moderate affinity, in which the Phe241 at the end of P's C-terminus plays a critical role in the binding of P peptide to N protein. Here, we found that the side-chain aromatic phenyl moiety of P Phe241 residue can form short- and long-range cation-π interactions with N Arg132 and Arg150 residues, respectively, as well as T-shaped and parallel-displaced π-π stackings with N Tyr135 and His151 residues, respectively, which co-define a geometrically satisfactory π-stacking system at the complex interface of N protein with P peptide, thus largely stabilizing the complex architecture. The stacking effect was further optimized by systematically mutating the P Phe241 residue to other natural and non-natural aromatic amino acids with diverse chemical substitutions at the phenyl moiety to examine their structural and energetic effects on π-stacking system and on protein-peptide binding. The electron-donating mutations at the phenyl moiety of P Phe241 residue can effectively enhance the π-stacking system and then promote peptide binding, whereas the bulky and positively charged mutations would considerably impair the peptide potency by introducing steric hindrance and electrostatic repulsion. The [Tyr]P, [Thp]P and [Fph]P mutants were determined to have an increased affinity relative to wild-type P peptide, which could be used as self-inhibitory peptides to competitively disrupt the native interaction between N and P proteins.


Assuntos
Vírus Sincicial Respiratório Humano , Humanos , Criança , Vírus Sincicial Respiratório Humano/genética , Vírus Sincicial Respiratório Humano/química , Vírus Sincicial Respiratório Humano/metabolismo , Fosfoproteínas/química , Fosfoproteínas/genética , Fosfoproteínas/metabolismo , Nucleocapsídeo/metabolismo , Nucleoproteínas , Peptídeos/química
2.
Cancer Lett ; 552: 215977, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36279983

RESUMO

IL-17-producing CD8 (Tc17) T cells have been shown to play an important role in infection and chronic inflammation, however their implications in hepatocellular carcinoma (HCC) remain elusive. In this study, we performed cytometry by time-of-flight (CyTOF) and revealed the distinctive immunological phenotypes of two IFNγ+ and IFNγ- Tc17 subsets that were preferentially enriched in human HCC. Single-cell RNA-sequencing analysis further revealed regulatory circuits governing the different phenotypes of these Tc17 subsets. In particular, we discovered that IFNγ- Tc17 subset demonstrated pro-tumoral characteristics and expressed higher levels of CCL20. This corresponded to increased tumor infiltration of T regulatory cells (Treg) validated by immunohistochemistry in another independent HCC cohort, demonstrating the immunosuppressive functions of IFNγ- Tc17 subset. Most importantly, higher intra-tumoral proportions of IFNγ- Tc17 were associated with poorer prognosis in patients with HCC and this was further validated in The Cancer Genome Atlas (TCGA) HCC cohort. Taken together, this compendium of transcriptomic and proteomic data of Tc17 subsets sheds light on the immunosuppressive phenotypes of IFNγ- Tc17 and its implications in HCC progression.


Assuntos
Carcinoma Hepatocelular , Tolerância Imunológica , Neoplasias Hepáticas , Humanos , Carcinoma Hepatocelular/imunologia , Carcinoma Hepatocelular/patologia , Linfócitos T CD8-Positivos , Interferon gama , Interleucina-17/genética , Neoplasias Hepáticas/imunologia , Neoplasias Hepáticas/patologia , Proteômica
3.
Appl Psychol Meas ; 47(1): 64-75, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36425286

RESUMO

Diagnostic classification models (DCMs) have been used to classify examinees into groups based on their possession status of a set of latent traits. In addition to traditional item-based scoring approaches, examinees may be scored based on their completion of a series of small and similar tasks. Those scores are usually considered as count variables. To model count scores, this study proposes a new class of DCMs that uses the negative binomial distribution at its core. We explained the proposed model framework and demonstrated its use through an operational example. Simulation studies were conducted to evaluate the performance of the proposed model and compare it with the Poisson-based DCM.

4.
Neuroradiology ; 2022 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-36447059

RESUMO

PURPOSE: To characterize the altered brain function in patients with vestibular migraine (VM) using resting-state functional magnetic resonance imaging (fMRI). METHODS: In this prospective study, fMRI images as well as clinical characteristics and behavioral scales were collected from 40 VM patients and 40 healthy controls (HC). All patients received neurological, neuro-otological, and conventional MRI examinations to exclude peripheral vestibular lesions, focal lesions, and other neurological diseases. Seed-based (bilateral parietal operculum cortex 2, OP2) functional connectivity (FC) and independent component analysis (ICA)-based functional network connectivity (FNC) were performed to investigate the brain functional changes in patients with VM. Additionally, the correlations between the altered FC/FNC and behavioral results were analyzed. RESULTS: Compared with HC, patients with VM showed increased FC between the left OP2 and right precuneus and exhibited decreased FC between the left OP2 and left anterior cingulate cortex. We also observed increased FC between the right OP2 and regions of the right middle frontal gyrus and bilateral precuneus, as well as decreased FC between the bilateral OP2. Furthermore, patients with VM showed decreased FNC between visual network (VN) and networks of auditory and default mode, and exhibited increased FNC between VN and executive control network. A correlation analysis found that FC between the left OP2 and right precuneus was positively correlated with scores of dizziness handicap inventory (DHI) in patients with VM. CONCLUSION: The present study demonstrated altered brain function in patients with VM.

5.
BMC Psychiatry ; 22(1): 744, 2022 11 30.
Artigo em Inglês | MEDLINE | ID: mdl-36451150

RESUMO

BACKGROUND: Major depressive disorder (MDD) is a high risk factor for suicide, with up to 20% of MDD patients attempting suicide during their lifetime. Current treatments for MDD are slow onset of action, low efficiency, and the inability to control suicidal behaviors quickly and effectively. Intravenous ketamine has been shown to have a rapid but transient antidepressant effect, but there is still lack evidence on the efficacy and safety of intravenous esketamine in reducing suicidal ideation and depressive symptoms in MDD patients with suicidal ideation. We designed a study to investigate the effect of short-term repeated intravenous infusion of esketamine three times in MDD patients with suicidal ideation. METHODS: This study features a randomized, double-blind, placebo-controlled trial (RCT) comparing short-term repeated intravenous infusions of esketamine with placebo as a supplement to conventional antidepressants with an intervention period of 6 days and one infusion every other day, followed by 4 weeks of follow-up. These methods support the examination of the efficacy, safety, tolerability, and mechanism of action of short-term repeated intravenous infusions of esketamine in MDD patients with suicidal ideation. DISCUSSION: This is the first RCT to explore the efficacy and safety of short-term repeated infusion of esketamine on suicidal ideation and depressive symptoms in MDD patients with suicidal ideation. If proven effective and tolerated, it will provide evidence for rapid and effective treatment of suicidal ideation and depressive symptoms in MDD individuals with suicidal ideation. TRIAL REGISTRATION: Chinese Clinical Trial Register, ChiCTR2000041232 . Registered 22 December 2020.


Assuntos
Transtorno Depressivo Maior , Ketamina , Suicídio , Humanos , Ideação Suicida , Ketamina/efeitos adversos , Transtorno Depressivo Maior/tratamento farmacológico , Ensaios Clínicos Controlados Aleatórios como Assunto
6.
Animals (Basel) ; 12(22)2022 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-36428331

RESUMO

A 60-day feeding trial was performed to assess the effects of dietary phosphorus levels on growth performance, body composition, phosphorus utilization, plasma physiological parameters and intestinal Ca and P transport-related gene expression of juvenile Chinese soft-shelled turtle (P. sinensis). Four diets containing available P at graded levels of 0.88%, 1.00%, 1.18% and 1.63% (termed as D0.88, D1.00, D1.18 and D1.63, respectively) were formulated and each diet was fed to turtles (5.39 ± 0.02 g) in sextuplicate. The turtles were randomly distributed to 24 tanks with 8 turtles per tank. The results indicated that final body weight, specific growth rate, feed conversion ratio and protein efficiency ratio performed best in turtles fed 1.00% available P diet. The crude lipids of the whole body exhibited a decreasing trend with the dietary available P, whereas the calcium and phosphorus of the whole body and bone phosphorus showed an opposite tendency. The apparent digestibility coefficient of phosphorus declined with the dietary available P. Turtles fed 1.00% available phosphorus had the highest phosphorus retention ratio compared with other treatments. Simultaneously they had significantly lower phosphorus loss than turtles fed D1.18 and D1.63 and had no differences in this respect from turtles fed a low-phosphorus diet. It was noteworthy that the lowest plasma calcium concentrations, and alkaline phosphatase activities in plasma and liver, were discovered in turtles fed the diet containing 1.63% available phosphorus. In addition, the high-phosphorus diet resulted in significantly down-regulated expression of intestinal phosphorus and calcium transport-related key genes. In conclusion, the available phosphorus requirement of juvenile P. sinensis was determined at 1.041% (total phosphorus was 1.80%) based on quadratic regression of weight gain rate, and excessive dietary phosphorus stunted turtle growth possibly via inhibiting intestinal calcium absorption.

7.
Nurs Open ; 2022 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-36403131

RESUMO

AIM: The mediating role of emotional disorders between conflict management styles and work engagement was explored based on constructing structural equation models in paediatric nurses. DESIGN: A cross-sectional study. METHODS: According to a cross-sectional survey, 300 paediatric nurses were selected from three tertiary hospitals (Chang sha, China), the data were collected using demographic questionnaires, the Rahim Organizational Conflict Inventory-II, Depression, Anxiety and Stress Scales and the Utrecht Work Engagement Scale. The Structural Equation Model was employed to investigate the mediating role of emotional disorders between conflict management styles and work engagement. RESULTS: Among conflict management styles, emotional disorders and work engagement, the associations were all significant (p < .05). In the mediation models, emotional disorders partially mediate the relationships between conflict management styles and work engagement (indirect effect 0.095, p < .01; direct effect -0.330, p < .01; total effect -0.330, p < .01) and between conflict management styles and work engagement (indirect effect 0.095, p < .01; direct effect 0.329, p < .01; total effect 0.424, p < .01).

8.
Cancers (Basel) ; 14(22)2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36428745

RESUMO

Multiple myeloma (MM) is the second-most common hematologic malignancy and remains incurable despite potent plasma cell directed therapeutics. The tumor microenvironment (TME) is a key player in the pathogenesis and progression of MM and is an active focus of research with a view to targeting immune dysregulation. Tumor-associated macrophages (TAM), myeloid derived suppressor cells (MDSC), and dendritic cells (DC) are known to drive progression and treatment resistance in many cancers. They have also been shown to promote MM progression and immune suppression in vitro, and there is growing evidence of their impact on clinical outcomes. The heterogeneity and functional characteristics of myelomonocytic cells in MM are being unraveled through high-dimensional immune profiling techniques. We are also beginning to understand how they may affect and be modulated by current and future MM therapeutics. In this review, we provide an overview of the biology and clinical relevance of TAMs, MDSCs, and DCs in the MM TME. We also highlight key areas to be addressed in future research as well as our perspectives on how the myelomonocytic compartment of the TME may influence therapeutic strategies of the future.

9.
Nanomaterials (Basel) ; 12(21)2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36364702

RESUMO

Metal-organic frameworks (MOFs) have attracted extensive attention as precursors for the preparation of carbon-based materials due to their highly controllable composition, structure, and pore size distribution. However, there are few reports of MOFs using p-phenylenediamine (pPD) as the organic ligand. In this work, we report the preparation of a bimetallic MOF (CoCu-pPD) with pPD as the organic ligand, and its derived hollow carbon spheres (BMHCS). CoCu-pPD exhibits a hollow spherical structure assembled by nanosheets. BMHCS inherits the unique hollow spherical structure of CoCu-pPD, which also shows a large specific surface area and heteroatom doping. When using as the anode of sodium-ion batteries (SIBs), BMHCS exhibits excellent cycling stability (the capacity of 306 mA h g-1 after 300 cycles at a current density of 1 A g-1 and the capacity retention rate of 90%) and rate capability (the sodium storage capacity of 240 mA h g-1 at 5 A g-1). This work not only provides a strategy for the preparation of pPD-based bimetallic-MOFs, but also enhances the thermal stability of the pPD-based MOFs. In addition, this work also offers a new case for the morphology control of assembled carbon materials and has achieved excellent performance in the field of SIBs.

10.
Int J Clin Pract ; 2022: 8568724, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36380749

RESUMO

Background: To establish two nomograms to quantify the diagnostic factors of lung metastasis (LM) and their role in assessing prognosis in young patients with LM osteosarcoma. Methods: A total of 618 osteosarcoma young patients from 2010 to 2015 were included from the Surveillance, Epidemiology, and End Results (SEER) database. Another 131 patients with osteosarcoma from local hospitals were also collected as an external validation set. Patients were randomized into training sets (n = 434) and validation sets (n = 184) with a ratio of 7:3. Univariate and multivariate logistic regression analyses were used to identify the risk factor for LM and were used to construct the nomogram. Risk variables for the overall survival rate of patients with LM were evaluated by Cox regression. Another nomogram was also constructed to predict survival rates. The results were validated using bootstrap resampling and retrospective research on 131 osteosarcoma young patients from 2010 to 2019 at three local hospitals. Results: There were 114 (18.45%) patients diagnosed as LM at initial diagnosis. The multivariate logistic regression analysis suggested that T stage, N stage, and bone metastasis were independent risk factors for LM in newly diagnosed young osteosarcoma patients (P < 0.001). The ROC analysis revealed that area under the curve (AUC) values were 0.751, 0.821, and 0.735 in the training set, internal validation set, and external validation set, respectively, indicating good predictive discrimination. The multivariate Cox proportional hazard regression analysis suggested that age, surgery, chemotherapy, primary site, and bone metastasis were prognostic factors for young osteosarcoma patients with LM. The time-dependent ROC curves showed that the AUCs for predicting 1-year, 2-year, and 3-year survival rates were 0.817, 0.792, and 0.815 in the training set and 0.772, 0.807, and 0.804 in the internal validation set, respectively. As for the external validation set, the AUCs for predicting 1-year, 2-year, and 3-year survival rates were 0.787, 0.818, and 0.717. Conclusions: The nomograms can help clinicians strengthen their personal decision-making and can improve the prognosis of osteosarcoma patients.


Assuntos
Neoplasias Ósseas , Neoplasias Pulmonares , Osteossarcoma , Humanos , Nomogramas , Programa de SEER , Estudos Retrospectivos , Estadiamento de Neoplasias , Prognóstico , Neoplasias Ósseas/diagnóstico , Neoplasias Ósseas/secundário , Fatores de Risco
11.
ACS Omega ; 7(44): 40306-40315, 2022 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-36385901

RESUMO

To overcome the environmental and economic challenges posed by the increasing amounts of the coal gasification slag, here, a simple and efficient method for enriching the residual carbon from the coal gasification fine slag was proposed. The residual carbon enrichment pattern in the particle size distribution of coal gasification fine slags after the ultrasonic pretreatment was mainly enriched toward the 500-250 µm and 250-125 µm particle size classes by analyzing the changes in the particle size distribution and apparent morphology. The pulp pretreatment at the ultrasonic output power of 270 W for 4 min was determined as the optimal experimental condition with respect to the yield, ash content, and ash rejection of the concentrates. Compared to the conventional wet sieving separation, the yield and ash content of the final concentrates were reduced by 7.99 and 14.96%, respectively. Moreover, the ash rejection of the final concentrates was as high as 88.51%, indicating an increment of 11.63% than the conventional wet sieving separation. Furthermore, thermogravimetric analysis confirmed that the final concentrates exhibited the lowest reactivity; however, these demonstrated had the highest carbon content (nearly 70%) with 27.27% ash content. The combustion characteristics analysis showed that the wet screening concentrate after ultrasonic pretreatment had the highest composite combustion characteristic index (S) of 3.17 × 10-8, as compared to the raw and conventional sieving concentrates.

12.
Front Public Health ; 10: 1033432, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36330112

RESUMO

The COVID-19 epidemic has caused more than 6.4 million deaths to date and has become a hot topic of interest in different disciplines. According to bibliometric analysis, more than 340,000 articles have been published on the COVID-19 epidemic from the beginning of the epidemic until recently. Modeling infectious diseases can provide critical planning and analytical tools for outbreak control and public health research, especially from a spatio-temporal perspective. However, there has not been a comprehensive review of the developing process of spatio-temporal dynamic models. Therefore, the aim of this study is to provide a comprehensive review of these spatio-temporal dynamic models for dealing with COVID-19, focusing on the different model scales. We first summarized several data used in the spatio-temporal modeling of the COVID-19, and then, through literature review and summary, we found that the existing COVID-19 spatio-temporal models can be divided into two categories: macro-dynamic models and micro-dynamic models. Typical representatives of these two types of models are compartmental and metapopulation models, cellular automata (CA), and agent-based models (ABM). Our results show that the modeling results are not accurate enough due to the unavailability of the fine-grained dataset of COVID-19. Furthermore, although many models have been developed, many of them focus on short-term prediction of disease outbreaks and lack medium- and long-term predictions. Therefore, future research needs to integrate macroscopic and microscopic models to build adaptive spatio-temporal dynamic simulation models for the medium and long term (from months to years) and to make sound inferences and recommendations about epidemic development in the context of medical discoveries, which will be the next phase of new challenges and trends to be addressed. In addition, there is still a gap in research on collecting fine-grained spatial-temporal big data based on cloud platforms and crowdsourcing technologies to establishing world model to battle the epidemic.


Assuntos
COVID-19 , Epidemias , Humanos , COVID-19/epidemiologia , Simulação por Computador , Surtos de Doenças , Previsões
13.
Geriatr Nurs ; 48: 154-159, 2022 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-36219935

RESUMO

This cross-sectional study aimed to explore the relationship between diet quality, dietary diversity, and oxidative stress levels in older adults in parts of the Ningxia Hui Autonomous Region in northwestern China. A total of 335 participants voluntarily participated in the study from April to July 2021. Laboratory tests and questionnaires were used to obtain general characteristics, dietary conditions, and indicators of oxidative stress. The dietary diversity scores of the participants were 5.20±1.39, the diet quality indices were 56.91±11.14, and most had poor diet quality. The levels of the oxidative stress markers malondialdehyde, superoxide dismutase, 8-iso-prostaglandin F2α, and total antioxidant capacity were 4.77±1.77, 40.33±9.85, 763.10±245.41, and 1.02±0.14, respectively. The results showed that the diet quality scores of participants were related to dietary diversity and whether the scores passed or not was significantly related to total antioxidant capacity.

14.
Front Neurosci ; 16: 956056, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36188452

RESUMO

Background: Ketamine, a robust antidepressant, has promising potential in the treatment of major depressive disorder (MDD). However, it does not work for all MDD patients, and the mechanism underlying its anti-depressive effects is unclear. Researchers have explored the mechanisms of ketamine action in MDD patients through MRI, a technique that measures brain activity intuitively. Notably, many MRI results were inconsistent because they selected different brain regions as seeds, particularly with respect to functional connectivity (FC) analysis. To eliminate the influence of prior seeds as much as possible, we used the significantly different results in degree centrality (DC) analysis as seeds to explore the FC changes in MDD patients to identify an imaging biomarker of ketamine's effect. Methods: Forty-four MDD patients and 45 healthy controls (HCs) were included in the study. Patients, aged 18-65, received six intravenous ketamine injections over 12 days. Depressive symptoms were estimated and MRI scans were performed at baseline and the day after the sixth infusion. We estimated FC differences between responders, non-responders and HCs using the region that showed significant differences between responders and non-responders in DC analysis as the seed. The correlation between the MADRS changes and zFC values was performed, and the potential of zFC values to be a neuroimaging biomarker was explored using the receiver operating characteristic curve. Result: Compared with non-responders, responders had significantly decreased DC values in the right middle frontal gyrus (MFG). In the analysis of FC using the region that showed significant differences in DC as a seed, there was a significant difference in the region of the right supplementary motor area (SMA) among responders, non-responders, and HCs. This region also overlapped with the bilateral median cingulate gyrus. In post hoc analysis, responders had higher FC than non-responders and HCs, and non-responders had lower FC than HCs. Importantly, the FC between the MFG and SMA (overlapping bilateral median cingulate gyrus) was correlated with the improvement of symptoms, which was estimated by the Mongomery-Asberg Depression Scale (MADRS). FC has the potential to be an imaging biomarker that can predict the ketamine effect in MDD patients according to the receiver operating characteristic curve analysis. Conclusion: Our results revealed that FC between the SMG and SMA and mACC was highly correlated with depressive symptoms and has the potential to be a neuroimaging biomarker to predict the effect of ketamine in MDD.

15.
Artigo em Inglês | MEDLINE | ID: mdl-36239896

RESUMO

Hydroxyapatite-coated quartz sands were synthesized by the sol-gel method and employed as a permeable reactive barrier (PRB) medium for the manganese contaminated aqueous solution treatment. The effects of composite particle size, initial concentration of manganese, and hydraulic load on the manganese removal in aqueous solution were investigated by column test. The Thomas and Yoon-Nelson dynamic models were used to reproduce the Mn(II) adsorption behavior observed in these column experiments. The scanning electron microscope (SEM) coupled with energy dispersive spectrometer (EDS), X-ray diffractometer (XRD), and X-ray photoelectron spectroscopy (XPS) were employed to investigate the Mn(II) removal mechanism. Results showed that the initial concentration of manganese had the greatest influence on Mn(II) removal when the initial concentration of manganese is 3 mg/L, the particle size is 0.15 ~ 0.3 mm, the hydraulic load is 5.5 m3/m2·d, and the adsorption capacity of the composites reached the maximum of 1.10 mg/g. The Thomas model fitted the breakthrough curves better. The maximum adsorption capacity of Mn(II) is 0.7546 mg/g. The adsorption mechanisms are mainly ion exchange and dissolution-precipitation. The results indicate that the hydroxyapatite-coated quartz sands could be an effective PRB media for the manganese-contaminated water treatment.

16.
Materials (Basel) ; 15(19)2022 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-36233954

RESUMO

This paper presents the reverse priority impedance control of manipulators with reference to redundant robots of a given task. The reverse priority kinematic control of redundant manipulators is first expressed in detail. The motion in the joint space is derived following the opposite order compared with the classical task priority-based solution. Then the Cartesian impedance control is combined with the reverse priority impedance control to solve the reverse hierarchical impedance controlled, so that the Cartesian impedance behavior can be divided into the primary priority impedance control and the secondary priority impedance control. Furthermore, the secondary impedance control task will not disturb the primary impedance control task. The motion in the joint space is affected following the opposite order and working in the corresponding projection operators. The primary impedance control tasks are implemented at the end, so as to avoid the possible deformations caused by the singularities occurring in the secondary impedance control tasks. Hence, the proposed reverse priority impedance control of manipulator can achieve the desired impedance control tasks with proper hierarchy. In this paper, the simulation experiments of the manipulator will verify the proposed reverse priority control algorithm.

17.
Biomed Res Int ; 2022: 9223954, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36193300

RESUMO

Background: Ovarian cancer (OC) is a common clinical gynecological disease, which seriously threatens women's health and life. We investigated the roles of SPOCK2 in OC and its associated molecular mechanism in the current study. Methods: The expressions and prognostic value of SPOCK2 in OC were identified using the clinical data and data from the GEPIA database. Then, SPOCK2 silence was implemented to search functions of SPOCK2 in OC cells. CCK-8 was used to examine cell proliferation. Cell apoptosis was detected by flow cytometry. The OC cell invasion and migration were evaluated by transwell assays. Results: Overexpressed SPOCK2 was identified in OC, which was correlated with poor prognosis and a shorter survival rate. SPOCK2 downregulation significantly suppressed OC cell proliferation, migration, and invasion, and cell apoptosis was markedly promoted by SPOCK2 silence. Meanwhile, SPOCK2 knockdown could effectively suppress Wnt/ß-catenin. Conclusion: SPOCK2 exerted crucial functions in OC progression and could serve as a promising candidate for OC targeted therapy.


Assuntos
Neoplasias Ovarianas , Via de Sinalização Wnt , Carcinoma Epitelial do Ovário , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Neoplasias Ovarianas/patologia , Proteoglicanas/metabolismo , Sincalida/metabolismo , Via de Sinalização Wnt/genética , beta Catenina/genética , beta Catenina/metabolismo
18.
Front Genet ; 13: 975905, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36313456

RESUMO

Introduction: Molecular chaperones and long non-coding RNAs (lncRNAs) have been confirmed to be closely related to the occurrence and development of tumors, especially lung cancer. Our study aimed to construct a kind of molecular chaperone-related long non-coding RNAs (MCRLncs) marker to accurately predict the prognosis of lung adenocarcinoma (LUAD) patients and find new immunotherapy targets. Methods: In this study, we acquired molecular chaperone genes from two databases, Genecards and molecular signatures database (MsigDB). And then, we downloaded transcriptome data, clinical data, and mutation information of LUAD patients through the Cancer Genome Atlas (TCGA). MCRLncs were determined by Spearman correlation analysis. We used univariate, least absolute shrinkage and selection operator (LASSO) and multivariate Cox regression analysis to construct risk models. Kaplan-meier (KM) analysis was used to understand the difference in survival between high and low-risk groups. Nomogram, calibration curve, concordance index (C-index) curve, and receiver operating characteristic (ROC) curve were used to evaluate the accuracy of the risk model prediction. In addition, we used gene ontology (GO) enrichment analysis and kyoto encyclopedia of genes and genomes (KEGG) enrichment analyses to explore the potential biological functions of MCRLncs. Immune microenvironmental landscapes were constructed by using single-sample gene set enrichment analysis (ssGSEA), tumor immune dysfunction and exclusion (TIDE) algorithm, "pRRophetic" R package, and "IMvigor210" dataset. The stem cell index based on mRNAsi expression was used to further evaluate the patient's prognosis. Results: Sixteen MCRLncs were identified as independent prognostic indicators in patients with LUAD. Patients in the high-risk group had significantly worse overall survival (OS). ROC curve suggested that the prognostic features of MCRLncs had a good predictive ability for OS. Immune system activation was more pronounced in the high-risk group. Prognostic features of the high-risk group were strongly associated with exclusion and cancer-associated fibroblasts (CAF). According to this prognostic model, a total of 15 potential chemotherapeutic agents were screened for the treatment of LUAD. Immunotherapy analysis showed that the selected chemotherapeutic drugs had potential application value. Stem cell index mRNAsi correlates with prognosis in patients with LUAD. Conclusion: Our study established a kind of novel MCRLncs marker that can effectively predict OS in LUAD patients and provided a new model for the application of immunotherapy in clinical practice.

19.
Curr Psychol ; : 1-16, 2022 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-36313582

RESUMO

The economic impact caused by the outbreak and dynamic evolution of COVID-19 has reduced employees' psychological security (PS), which not only threatens the physical and mental health of employees but also seriously affects the stable operation and sustainable development of enterprises. PS has been determined to be closely related to daily life experiences. Therefore, the purpose of this article is to examine the types and combinations of life events that improve employees' PS during the pandemic. Cross-sectional data came from 764 enterprise employees in 8 provinces and cities in China during the pandemic period. The participants completed the PS scale to evaluate their PS, and the PS events scale to evaluate the different types of daily life events they experienced. Multiple regression analysis (MRA) and fuzzy-set qualitative comparative analysis (fsQCA) methods were used to test the research hypothesis. The results of MRA suggest that rich leisure activities (RLA), harmonious family relationship (HFR), stable economic order (SEO) and recognition and support from others (RSO) are important life events that enhance employees' PS. The results of fsQCA suggest that the independent role of SEO, the combined role of sound social security system (SSSS), peace and health events (PHE) and HFR, the combined role of PHE, fulfilling work/life status (FWLS), SEO and RSO can substitute for each other to promote employees' high PS. This article reveals the contribution of daily life events to the PS of enterprise employees, and provides an empirical basis for formulating corresponding intervention measures to promote the physical and mental health of enterprise employees and effective enterprise management.

20.
Neuroimage Clin ; 36: 103230, 2022 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-36274375

RESUMO

BACKGROUND: The default mode network (DMN) is implicated in the pathophysiology of major depressive disorder (MDD), and functional connectivity (FC) involved in DMN is suggested to be associated with antidepressant remission. The goal of this study is to recognize relationships between FC within DMN and early amelioration in MDD patients and to further test the capacity of FC to predict early efficacy. METHODS: In total 66 MDD patients and 57 healthy controls were recruited for resting-state functional magnetic resonance imaging scans at baseline. After four weeks of treatment with Escitalopram or Venlafaxine, patients were divided into subgroups with remitters (R, n = 31) and non-remitters (NR, n = 35). Independent component analysis (ICA) was used to compare intranetwork functional connectivity (intra-FC) in DMN between the three groups. RESULTS: Relative to NR-MDD group and HCs, the R-MDD group showed significantly higher intra-FC in the right angular gyrus of DMN, and the intra-FC was positively correlated with the reduction ratio of the depressive symptom scores. The ROC curve analysis revealed that intra-FC exhibited a high diagnostic value for remission. CONCLUSION: These findings indicated that intra-FC related to the DMN is a prognostic marker that can potentially predict early remission of symptoms after antidepressant treatment.

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