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1.
Nat Commun ; 13(1): 125, 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-35013285

RESUMO

Rechargeable Li-I2 battery has attracted considerable attentions due to its high theoretical capacity, low cost and environment-friendliness. Dissolution of polyiodides are required to facilitate the electrochemical redox reaction of the I2 cathode, which would lead to a harmful shuttle effect. All-solid-state Li-I2 battery totally avoids the polyiodides shuttle in a liquid system. However, the insoluble discharge product at the conventional solid interface results in a sluggish electrochemical reaction and poor rechargeability. In this work, by adopting a well-designed hybrid electrolyte composed of a dispersion layer and a blocking layer, we successfully promote a new polyiodides chemistry and localize the polyiodides dissolution within a limited space near the cathode. Owing to this confined dissolution strategy, a rechargeable and highly reversible all-solid-state Li-I2 battery is demonstrated and shows a long-term life of over 9000 cycles at 1C with a capacity retention of 84.1%.

2.
J Am Chem Soc ; 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-35007081

RESUMO

Enantioselective [3 + 2] annulation of N-heteroarenes with alkynes has been developed via a cobalt-catalyzed dearomative umpolung strategy in the presence of chiral ligand and reducing reagent. A variety of electron-deficient N-heteroarenes, including quinolines, isoquinolines, quinoxaline, and pyridines, and internal or terminal alkynes are employed in this reaction, showing a broad substrate scope and good functionality tolerance. Annulation of electron-rich indoles with alkynes is also developed. This protocol provides a straightforward access to a variety of N-spiroheterocyclic molecules in excellent enantioselectivities (76 examples, up to 99% ee).

3.
J Environ Sci (China) ; 115: 465-473, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-34969474

RESUMO

Cross-boundary transport of air pollution is a difficult issue in pollution control for the North China Plain. In this study, an industrial district (Shahe City) with a large glass manufacturing sector was investigated to clarify the relative contribution of fine particulate matter (PM2.5) to the city's high levels of pollution. The Nest Air Quality Prediction Model System (NAQPMS), paired with Weather Research and Forecasting (WRF), was adopted and applied with a spatial resolution of 5 km. During the study period, the mean mass concentrations of PM2.5, SO2, and NO2 were observed to be 132.0, 76.1, and 55.5 µg/m3, respectively. The model reproduced the variations in pollutant concentrations in Shahe at an acceptable level. The simulation of online source-tagging revealed that pollutants emitted within a 50-km radius of downtown Shahe contributed 63.4% of the city's total PM2.5 concentration. This contribution increased to 73.9±21.2% when unfavorable meteorological conditions (high relative humidity, weak wind, and low planetary boundary layer height) were present; such conditions are more frequently associated with severe pollution (PM2.5 ≥ 250 µg/m3). The contribution from Shahe was 52.3±21.6%. The source apportionment results showed that industry (47%), transportation (10%), power (17%), and residential (26%) sectors were the most important sources of PM2.5 in Shahe. The glass factories (where chimney stack heights were normally < 70 m) in Shahe contributed 32.1% of the total PM2.5 concentration in Shahe. With an increase in PM2.5 concentration, the emissions from glass factories accumulated vertically and narrowed horizontally. At times when pollution levels were severe, the horizontally influenced area mainly covered Shahe. Furthermore, sensitivity tests indicated that reducing emissions by 20%, 40%, and 60% could lead to a decrease in the mass concentration of PM2.5 of of 12.0%, 23.8%, and 35.5%, respectively.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Poluentes Atmosféricos/análise , Poluição do Ar/análise , China , Cidades , Monitoramento Ambiental , Material Particulado/análise , Estações do Ano
4.
Environ Pollut ; 292(Pt B): 118273, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34634404

RESUMO

Public places favor the transmission of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) due to dense population, large personal mobility, and higher contact opportunities. In order to protect the health of general public in operating public places during COVID-19 pandemic, this study proposed general precautions and control strategies from perspective of operation management, social distancing, cleaning and disinfection, and personal protection. In addition, with regard of risk level, specific precautions and control strategies were proposed for living service places, outdoor places, and confined places. The comprehensive application of above recommendations could effectively interrupt the spread of COVID-19, and protect the health of general public in public places. This study proposed general and specific precautions and control strategies in public places during COVID-19, and suggested further improvement of pandemic response.


Assuntos
COVID-19 , Pandemias , Desinfecção , Humanos , Distanciamento Físico , SARS-CoV-2
5.
Chemosphere ; 287(Pt 2): 132060, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34507145

RESUMO

Freshwater resilience is facing to an increasing challenge, while carbon neutral wastewater reclamation has been put onto agenda in more and more countries. The activated sludge-microfiltration (MF)-reverse osmosis (RO) process has been currently adopted for reclamation of municipal wastewater to high-grade product water (e.g. NEWater). However, the conventional activated sludge (CAS) unit in this process has the drawbacks of excessive sludge generation, high energy consumption, greenhouse gases (GHGs) emissions etc. To address these emerging issues, an integrated anaerobic fixed-film membrane bioreactor (AnfMBR)-RO-chlorination process was developed in this study. Results showed that about 99.9% of COD, 99.3% of phosphate and 95.3% of NH4+-N were removed in the AnfMBR-RO process, while breakpoint chlorination served as a polishing step when the NH4+-N concentration in RO permeate exceeded the typical NH4+-N concentration (e.g. 1 mg/L) of NEWater. The net energy consumption and total GHG emissions in the proposed integrated process were estimated to be 0.33 kWh/m3 and 310.2 g CO2e/m3 influent wastewater treated, respectively, which were 64% and 74% less than those in the current municipal wastewater reclamation process. Consequently, this study offers an alternative path to bring municipal wastewater reclamation one step closer to carbon neutrality and water sustainability.


Assuntos
Águas Residuárias , Purificação da Água , Anaerobiose , Reatores Biológicos , Carbono , Halogenação , Membranas Artificiais , Osmose , Eliminação de Resíduos Líquidos , Águas Residuárias/análise , Água
6.
Sci Total Environ ; 805: 150270, 2022 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-34536863

RESUMO

Polychlorinated biphenyls (PCBs), together with 11 other organic compounds, were initially listed as persistent organic pollutants (POPs) by the Stockholm Convention because of their potential threat to ecosystems and humans. In China, many monitoring studies have been undertaken to reveal the level of PCBs in environment since 2005 due to the introduced stricter environmental regulations. However, there are still significant gaps in understanding the overall spatial and temporal distributions of PCBs in China. This review systematically discusses the occurrence and distribution of PCBs in environmental matrices, organisms, and humans in China. Results showed that PCB contamination in northern and southern China was not significantly different, but the PCB levels in East China were commonly higher than those in West China, which might have been due to the widespread consumption of PCBs and intensive human activities in East China. Serious PCB contamination was found in e-waste disassembling areas (e.g., Taizhou of Zhejiang Province and Qingyuan and Guiyu of Guangdong Province). Higher PCB concentrations were also chronicled in megalopolises and industrial clusters. The unintentionally produced PCBs (UP-PCBs) formed during industrial thermal processes may play an increasingly significant role in PCB pollution in China. Low PCB levels were recorded in rural and underdeveloped districts, particularly in remote and high-altitude localities such as the Tibetan Plateau and the South China Sea. However, these data are limited. Human exposure to PCBs is closely related to the characteristics of environmental pollution. This review also discusses existing issues and future research prospects on PCBs in China. For instance, the accumulation characteristics and migration regularities of PCBs in food webs should be further studied. More investigations should be undertaken to assess the quantitative relationship between external and internal exposure to PCBs. For example, bioaccessibility and bioavailability studies should be supplemented to evaluate human health risks more accurately.


Assuntos
Bifenilos Policlorados , China , Ecossistema , Monitoramento Ambiental , Poluição Ambiental , Cadeia Alimentar , Humanos , Bifenilos Policlorados/análise
7.
Adv Mater ; : e2109203, 2021 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-34883530

RESUMO

Single-atom catalysts (SACs) are being witnessed a rapid development due to their high activity and selectivity toward diverse reactions. However, it remains a grand challenge in the general synthesis of SACs, particularly featuring identical chemical microenvironment and on the same support. Herein, we have developed a universal synthetic protocol to immobilize SACs to metal-organic frameworks (MOFs). Significantly, by means of SnO2 as a mediator or adaptor, not only different single-atom metal sites, such as Pt, Cu and Ni, etc., can be installed, but also the MOF supports can be changed (for example, UiO-66-NH2 , PCN-222, and DUT-67) to afford M1 /SnO2 /MOF architecture. Taking UiO-66-NH2 as a representative, the Pt1 /SnO2 /MOF exhibit ∼5 times higher activity toward photocatalytic H2 production than the corresponding Pt nanoparticles (∼2.5 nm) stabilized by SnO2 /UiO-66-NH2 . Remarkably, despite featuring identical parameters in chemical microenvironment and support in M1 /SnO2 /UiO-66-NH2 , the Pt1 catalyst possesses a hydrogen evolution rate of 2167 µmol·g-1 ·h-1 , superior to the Cu1 and Ni1 counterparts, which is attributed to the differentiated hydrogen binding free energies, as supported by density-functional theory (DFT) calculations. To our knowledge, this is the first report on the universal approach to the stabilization of SACs with identical chemical microenvironment on the identical support. This article is protected by copyright. All rights reserved.

8.
Sci Total Environ ; 808: 152074, 2021 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-34863759

RESUMO

The occurrence and risks of pesticides and their transformation products in soil at the manufacturing sites are "known unknowns." In this study, pesticides and their transformation products were screened in soil at 6 pesticide manufacturing sites across China using liquid and gas chromatography coupled with quadrupole time-of-flight mass spectrometry. The screening strategy can correctly identify 75% of 209 pesticides spiked at 50 ng g-1. A total of 212 pesticides were identified; 23.1% of pesticides detected were above 200 ng g-1, and the maximum concentration was 1.5 × 105 ng g-1. The risk quotients of 20% pesticides were greater than 1, and the maximum risk quotient of imidacloprid reached 6.3 × 104. The most recent site showed a larger number of pesticides with higher diversity, whereas older sites were dominated by organochlorine insecticides. The extended screen identified 163 transformation products with concentrations up to 6.6 × 104 ng g-1. Half of the transformation products had higher concentrations than their parent compounds, and metabolic ratios up to 371 were observed. The results of this study validate the prevalence of pesticides and their transformation products in soil at pesticide manufacturing sites. The results also highlight the importance of comprehensive screening at industrial sites and call for improved management and regulation of pesticide manufacturing, particularly for in-service facilities.

9.
Sci Rep ; 11(1): 23907, 2021 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-34903812

RESUMO

FIP200 is an essential autophagy gene implicated in the regulation of postnatal neural progenitor/stem cells (NSCs). However, the contribution of FIP200's canonical-autophagy function and its non-canonical functions to postnatal NSC maintenance remains unclear. Utilizing a recently generated Fip200-4A allele that specifically impairs FIP200's canonical-autophagy function, we found that non-canonical functions of FIP200 was required for regulation of mouse NSC maintenance and neurogenesis in vivo. Ablating the non-canonical functions of FIP200, but not its autophagy function, increased TBK1 activation and p62 phosphorylation at S403 in NSCs. Phosphorylation of p62 was dependent on TBK1 kinase activity and increased the propensity of p62 aggregate formation specifically in FIP200-null NSCs. Accordingly, inhibition of TBK1 by amlexanox reduced p62 aggregates and restored NSC maintenance and differentiation in Fip200hGFAP cKO mice. These results reveal a mechanism for the non-canonical functions of FIP200 in NSC maintenance and differentiation by limiting TBK1 activation and subsequently, p62 aggregate formation.

10.
Zhongguo Zhong Yao Za Zhi ; 46(23): 6289-6293, 2021 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-34951256

RESUMO

The ripe dried fruit of citron(Citrus medica) is one of the important sources of Chinese herb Citri Fructus. At the same time, it is also grown for edible and ornamental uses. There are many species and abundant genetic variation. To clarify the intraspecific variation and resource distribution of citron, this study investigated the variation in 11 citron fruits, basically covering the main species in China, including Xiaoguo citron(C. medica var. ethrog), Goucheng(C. medica var. yunnanensis), Muli citron(C.medica var. muliensis), Dehong citron(C.medica×Citrus spp.), Fuzhou citron(C.medica×C.grandis?), Mawu(C.medica×C.grandis?), Cangyuan citron, Binchuan citron, Sweet citron, Big citron, and Small citron. The natural communities of citron were proved to be mainly distributed in the southwestern and western Yunnan and southeastern Tibet of China, with Yunnan, Sichuan, Guangxi, Chongqing, Hubei, and Zhejiang identified as the main production areas. Citron has also been widely grown in India, the Mediterranean region, and the Caribbean coast countries. The field investigation revealed the large-scale intraspecific variation of citron fruits. Most of the fruits are oval-like or sphere-like in shape. The fruits are green when raw and yellow when ripe, with oil cell dots on the skin, stripe-likes running from top to bottom, and bulge at the top. Usually, in the smaller citron fruits, the pulp and juice vesicles are better developed and the central columella is tighter. By contrast, the juice vesicles and central columella in larger fruits became more vacant, with carpels visible, and the apex segregation and development of the carpels is one of the reasons for variation. These variations should be given top priority in the future variety selection and breeding, and the quality differences of different citron species and their mechanisms should be further studied. In particular, variety selection and classification management according to their medicinal or edible purposes will provide scientific and technological supports for the orderly, safe, and effective production of citron products consumed as food and medicine.


Assuntos
Citrus , Frutas , China , Paladar , Tibet
11.
Artigo em Inglês | MEDLINE | ID: mdl-34962341

RESUMO

2D metal-organic-framework (MOF) based composites have emerged as promising candidates for electrocatalysis due to their high structural flexibility and fully exposed active sites. Herein, a freestanding metal organic layer (MOL) with a 2D kgd (kagome dual) lattice was constructed with abundant surface oxygenate groups serving as anchoring sites to immobilize diverse guests. Taking Bi as an example, tetragonal Bi 2 O 3 nanowires can be uniformly grown on MOLs after solvothermal treatment, the structural evolution of which was followed by ex-situ electron microscopy. The as-prepared Bi 2 O 3 /MOL exhibits excellent CO 2 electroreduction activity towards formate reaching a specific current of 2.3 A·mg Bi -1 and Faradaic efficiencies of over 85% with a wide potential range from -0.87 to -1.17 V, far surpassing Bi 2 O 3 /UiO (a 3D Zr 6 -oxo based MOF) and Bi 2 O 3 /AB (Acetylene Black). Such a post-synthetic modification strategy can be flexibly extended to develop versatile MOL composites, highlighting the superiority of optimizing MOL based composites for electrocatalysis.

12.
Front Cell Dev Biol ; 9: 774401, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34901019

RESUMO

Coordinated AP-1 and clathrin coat assembly mediate secretory sorting on the trans-Golgi network (TGN) during conventional secretion. Here we found that SMAP-1/SMAPs deficiency caused the apical protein ERM-1 to accumulate on the basolateral side of the TGN. In contrast, the basolateral protein SLCF-1 appeared abnormally on the apical membrane. SMAP-1 colocalized with AP-1 on the TGN. The integrity of AP-1 is required for the subcellular presence of SMAP-1. Moreover, we found that the loss of SMAP-1 reduced clathrin-positive structures in the cytosol, suggesting that SMAP-1 has a regulatory role in clathrin assembly on the TGN. Functional experiments showed that overexpressing clathrin effectively alleviated exocytic defects due to the lack of SMAP-1, corroborating the role of SMAP-1 in promoting the assembly of clathrin on the TGN. Together, our results suggested that the AP-1 complex regulates the TGN localization of SMAP-1, promoting clathrin assembly to ensure polarized conventional secretion in C. elegans intestinal epithelia.

13.
Int J Implant Dent ; 7(1): 115, 2021 11 29.
Artigo em Inglês | MEDLINE | ID: mdl-34842993

RESUMO

PURPOSE: To evaluate the clinical effects of concentrated growth factors (CGFs) combined with bone substitutes for alveolar ridge preservation (ARP) in the maxillary molar area. METHODS: Thirty-six patients who underwent extraction of the upper molars were recruited and randomly divided into three groups: 1. Grafted with CGFs combined with deproteinized bovine bone mineral (DBBM) and covered with CGFs membrane (CGFs/DBBM group), 2. Grafted with DBBM alone and covered with collagen membrane (DBBM group), 3. Control group spontaneous healing. The area of the alveolar bone in center (C-), mesial (M-) and distal (D-) section was compared with preoperative in radiography. Bone cores were obtained for histopathology observation and comparison. RESULTS: In C-, M- and D-section, the alveolar ridge area in all three groups was significantly reduced at 8 months postoperatively compared to the baseline (P < 0.05). The alveolar ridge area declines in the CGFs/DBBM group (C-12.75 ± 2.22 mm2, M-14.69 ± 2.82 mm2, D-16.95 ± 4.17 mm2) and DBBM group (C-14.08 ± 2.51 mm2, M-15.42 ± 3.47 mm2, D-16.09 ± 3.97 mm2) were non-significant differences. They were significantly less than the decline in the control group (C-45.04 ± 8.38 mm2 M-31.98 ± 8.34 mm2, D-31.85 ± 8.52 mm2) (P < 0.05). The percentage of newly formed bone in the CGFs/DBBM group (41.99 ± 12.99%) was significantly greater than that in DBBM group (30.68 ± 10.95%) (P < 0.05). The percentage of residual materials in the CGFs/DBBM group (16.19 ± 6.63%) was significantly less than that in the DBBM group (28.35 ± 11.70%) (P < 0.05). CONCLUSION: Combined application of CGFs and DBBM effectively reduced the resorption of alveolar ridge and resulted in more newly formed bone than the use of DBBM with collagen membranes.

14.
Mater Horiz ; 8(8): 2216-2229, 2021 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-34846426

RESUMO

Ferroptosis, a newly recognized form of non-apoptotic cell death, has recently been introduced for effective cancer therapy. The reported ferroptosis-inducing nanomaterials mainly consisted of metal-based components. Herein, we designed an inorganic metal-free nanoplatform, PSMA-targeted arsenic nanosheets (PMANs), which simultaneously increased glutathione (GSH) consumption, suppressed solute carrier family 7 member 11 (SLC7A11) and glutathione-dependent peroxidase 4 (GPX4) expression, and promoted the generation of reactive oxygen species (ROS) and lipid peroxides (LPO). In addition, owing to the large surface area, PMANs efficiently transported doxorubicin (DOX) to prostate cancer for synergistic therapy. Surprisingly, we found that PMANs could sensitize prostate cancer cells to DOX through downregulating the expression of ataxia telangiectasia mutated (ATM), which further augmented the GPX4 downregulation-mediated ferroptotic tumoricidal effect. Given that arsenic trioxide has been routinely and successfully used in the clinical treatment of leukemia for a long time, we anticipate that PMANs will offer a promising strategy for prostate cancer therapy.

15.
Chemosphere ; : 133072, 2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34838833

RESUMO

Organophosphorus flame retardants (OPFRs) have been regarded as one of the most rebarbative classes of emerging contaminants due to their persistence and toxicity. In the current study, Fe-based metal organic framework (MIL-88A) was synthesized and employed as photo-Fenton catalyst for the degradation of tris-(2-chloroisopropyl) phosphate (TCPP), a typical representative of OPFRs. The observations indicated that visible light could boost the reduction of ≡FeIII to ≡FeII in Fe-O clusters of MIL-88A during the photo-Fenton system and consequently induce the transformation of H2O2 to OH, which realized efficient degradation of TCPP. Due to the excellent function of MIL-88A, the effective pH application range of photo-Fenton system was extended in comparison with traditional Fenton system. The degradation efficiency of TCPP was visibly influenced in presence of humic acid (HA). MIL-88A exhibited a commendable reusability and stability after 3 times cycle. As the photo-Fenton reaction proceeded, TCPP was disintegrated to several kinds of carboxylated, dechlorinated and hydroxylated products. The observations of metabolomics endorsed that the interference of intermediate products mixture on E. coli weakened to a certain extent. In conclusion, carboxylation, dechlorination, hydroxylation and oxidation of TCPP were likewise effective for its detoxification, revealing that heterogeneous photo-Fenton system driven by Fe-based metal organic framework will be an attractive and safe treatment technique for OPFRs control.

16.
Cell Res ; 2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34789895

RESUMO

A major obstacle in Alzheimer's disease (AD) research is the lack of predictive and translatable animal models that reflect disease progression and drug efficacy. Transgenic mice overexpressing amyloid precursor protein (App) gene manifest non-physiological and ectopic expression of APP and its fragments in the brain, which is not observed in AD patients. The App knock-in mice circumvented some of these problems, but they do not exhibit tau pathology and neuronal death. We have generated a rat model, with three familiar App mutations and humanized Aß sequence knocked into the rat App gene. Without altering the levels of full-length APP and other APP fragments, this model exhibits pathologies and disease progression resembling those in human patients: deposit of Aß plaques in relevant brain regions, microglia activation and gliosis, progressive synaptic degeneration and AD-relevant cognitive deficits. Interestingly, we have observed tau pathology, neuronal apoptosis and necroptosis and brain atrophy, phenotypes rarely seen in other APP models. This App knock-in rat model may serve as a useful tool for AD research, identifying new drug targets and biomarkers, and testing therapeutics.

17.
Int J Biol Macromol ; 2021 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-34742552

RESUMO

The influence of inulin (IN) on pasting, thermal, rheological properties, and in vitro digestibility of pea starch gel was investigated. Results showed that as the concentration of IN in PS increased, the pasting temperature of PS gradually increased, while the value of peak viscosity, breakdown, and setback decreased. Rheological test suggested that all PS-IN gels were typical non-Newtonian fluids and exhibited a solid-like behavior. With the increased concentration of IN, hardness, chewiness, and gumminess of PS-IN gels significantly declined, in which the minimum value was at addition level of 20%. The presence of IN increased the gelatinization temperatures of PS-IN gels, while decreased the gelatinization enthalpy. Fourier-transform infrared spectroscopy (FT-IR) results indicated that no covalent bonding but intermolecular hydrogen bonding occurred between PS and IN. No influence of IN on the diffraction peak of PS after pasting was confirmed by X-ray diffraction analysis. In addition, IN decreased the content of rapidly and slowly digestible starch of PS, while increasing the content of resistant starch. These results will expand the application range of PS, and also provide a theoretical basis for the development of inulin-starch based products.

18.
Zool Res ; 42(6): 834-844, 2021 11 18.
Artigo em Inglês | MEDLINE | ID: mdl-34766482

RESUMO

Understanding the zoonotic origin and evolution history of SARS-CoV-2 will provide critical insights for alerting and preventing future outbreaks. A significant gap remains for the possible role of pangolins as a reservoir of SARS-CoV-2 related coronaviruses (SC2r-CoVs). Here, we screened SC2r-CoVs in 172 samples from 163 pangolin individuals of four species, and detected positive signals in muscles of four Manis javanica and, for the first time, one M. pentadactyla. Phylogeographic analysis of pangolin mitochondrial DNA traced their origins from Southeast Asia. Using in-solution hybridization capture sequencing, we assembled a partial pangolin SC2r-CoV (pangolin-CoV) genome sequence of 22 895 bp (MP20) from the M. pentadactyla sample. Phylogenetic analyses revealed MP20 was very closely related to pangolin-CoVs that were identified in M. javanica seized by Guangxi Customs. A genetic contribution of bat coronavirus to pangolin-CoVs via recombination was indicated. Our analysis revealed that the genetic diversity of pangolin-CoVs is substantially higher than previously anticipated. Given the potential infectivity of pangolin-CoVs, the high genetic diversity of pangolin-CoVs alerts the ecological risk of zoonotic evolution and transmission of pathogenic SC2r-CoVs.


Assuntos
COVID-19/veterinária , Evolução Molecular , Pangolins/virologia , SARS-CoV-2/genética , Animais , Genoma Viral , Filogenia , RNA Viral/genética
19.
Int J Mol Sci ; 22(21)2021 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-34769361

RESUMO

In the present study, four large-scale field trials using two doubled haploid wheat populations were conducted in different environments for two years. Grain protein content (GPC) and 21 other yield-related traits were investigated. A total of 227 QTL were mapped on 18 chromosomes, which formed 35 QTL clusters. The potential candidate genes underlying the QTL clusters were suggested. Furthermore, adding to the significant correlations between yield and its related traits, correlation variations were clearly shown within the QTL clusters. The QTL clusters with consistently positive correlations were suggested to be directly utilized in wheat breeding, including 1B.2, 2A.2, 2B (4.9-16.5 Mb), 2B.3, 3B (68.9-214.5 Mb), 4A.2, 4B.2, 4D, 5A.1, 5A.2, 5B.1, and 5D. The QTL clusters with negative alignments between traits may also have potential value for yield or GPC improvement in specific environments, including 1A.1, 2B.1, 1B.3, 5A.3, 5B.2 (612.1-613.6 Mb), 7A.1, 7A.2, 7B.1, and 7B.2. One GPC QTL (5B.2: 671.3-672.9 Mb) contributed by cultivar Spitfire was positively associated with nitrogen use efficiency or grain protein yield and is highly recommended for breeding use. Another GPC QTL without negatively pleiotropic effects on 2A (50.0-56.3 Mb), 2D, 4D, and 6B is suggested for quality wheat breeding.

20.
Technol Cancer Res Treat ; 20: 15330338211056478, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34806464

RESUMO

Purpose: In the present study, we mainly aimed to evaluate the prognostic value of 2-deoxy-2-[18F]fluoro-D-glucose ([18F]F-FDG) positron emission tomography (PET)/computed tomography (CT) after allogeneic stem cell transplantation (allo-SCT) in lymphoblastic lymphoma (LBL) patients using Deauville Scores (DS). Materials and Methods: A total of 63 LBL patients who benefited from 18F-FDG PET-CT after allo-SCT in our institution between April 2010 and August 2020 were enrolled in this retrospective study. These above-mentioned patients were divided into two groups based on the Deauville criteria. Diagnostic efficiency of 18F-FDG PET/CT and integrated CT in detecting lymphoma were calculated. Consistencies were evaluated by comparing 18F-FDG PET/CT and integrated CT results through kappa coefficient. Kaplan-Meier method was used in survival analysis, and the log-rank method was adopted in comparisons. Prognostic factor analysis was performed by the Cox regression model. Results: The sensitivity, specificity, positive predictive value, negative predictive value, accuracy of post-SCT 18F-FDG PET-CT were 100%(12/12), 92.2%(47/51), 75.0%(12/16), 100%(47/47) and 93.7%(59/63). The consistency of 18F-FDG PET-CT and integrated CT was moderate(Kappa = .702,P < .001). Positive post-SCT 18F-FDG PET-CT was associated with lower progression-free survival (PFS) but not overall survival (OS) (p = .000 and p = .056, respectively). The 3-year PFS of the PET-positive group and PET-negative group was 18.8% and 70.2%, respectively. Multivariate analysis showed that post-SCT PET-CT findings was an independent prognostic factor for PFS (p = .000; HR, 3.957; 95%CI, 1.839-8.514). Other factors independently affecting PFS were sex (p = .018; HR, 2.588; 95% CI, 1.181 - 5.670) and lactate dehydrogenase (LDH) (p = .005; HR, 3.246; 95% CI, 1.419 - 7.426). However, none of the above-mentioned factors were associated with OS. Conclusions: Collectively, we found that 18F-FDG PET-CT after allo-SCT was a strong indicator for PFS, but not OS, which might provide important evidence for the selection of subsequent treatment regimen for LBL patients. Trial registration number: ChiCTR2100046709.

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