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1.
Ticks Tick Borne Dis ; 12(3): 101679, 2021 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-33578256

RESUMO

Ticks and tick-borne rickettsial diseases have been gaining greater attention in China over the past decade. However, most published studies to date have occurred in Northern China, with limited investigations occurring in China's southern provinces. As part of larger surveillance efforts, a cross-sectional survey was conducted in six sites at Guangdong, Guangxi and Yunnan investigating rickettsial infection in ticks. A total of 581 ticks were collected from hosts and screened via PCR, targeting rrs, gltA, ompB, sca4, and ompA gene fragments. Two of 12 Haemaphysalis formosensis ticks were infected with novel Rickettsia strain GD01, which was closest phylogenetically (97.3-98.9 % identity) to Rickettsia tamurae strain AT-1, but not within the same clade. Another detected strain (GD02) shared similar identity, 99-100 % across four gene targets, to recently detected Candidatus Rickettsia longicornii isolate ROK-HL727, with an overall prevalence of 12.5 % (71/569). The presence of such pathogens calls for increased public health attention and active surveillance in patients reporting recent tick bites.

2.
Bioresour Technol ; 319: 124131, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33002784

RESUMO

In this study, the multiple effects of granular activated carbon (GAC) on sludge anaerobic digestion at ambient (16-24 °C), mesophilic (35 °C) and thermophilic (55 °C) temperature were investigated. After GAC addition, although the methane yields of raw sludge were reduced by 6.5%-36.9%, the lag phases of methanogenesis were shortened by 19.3%-30.6% and the reductions of methane yields were declined to only 5.9%-8.1% simultaneously for pretreated sludge. The inhibitory substances like phenols that generated by thermal pretreatment were reduced after GAC addition, which were demonstrated to be responsible for the methanogenic acceleration. Meanwhile, the methane reduction due to the non-selective adsorption by GAC could be mitigated by pretreatment and elevated temperature. Thus, a strategy coupling thermal pretreatment with detoxification by GAC was proposed to improve the methane production rate and avoid the negative effects during sludge anaerobic digestion with GAC addition.


Assuntos
Metano , Esgotos , Aceleração , Anaerobiose , Reatores Biológicos , Carvão Vegetal
3.
Zhongguo Zhong Yao Za Zhi ; 45(12): 2858-2864, 2020 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-32627460

RESUMO

Pre-formulation physicochemical properties of the component-based Chinese medicine of Qinqi Fengshi Fang were investigated to provide a research basis for the design of the dosage form for component-based Chinese medicine of Qinqi Fengshi Fang. The macroporous resin adsorption and refining technology was used to prepare the total glycosides extract of Gentianae Macrophyllae Radix, Panacis Majoris Rhizome and Corni Fructus respectively in the prescription of Qinqi Fengshi Fang. Their physicochemical properties were investigated, including solubility, wettability, hygroscopicity, equilibrium solubility, oil-water partition coefficient, and stability. The results showed that the total glycosides of Gentianae Macrophyllae Radix, Panacis Majoris Rhizome and Corni Fructus all had good solubility and wettability. The solubility index of each total glycoside component was greater than 85%, and the water absorption index was greater than 50%. In the range of pH 2.0-7.4, the equilibrium solubility of three kinds of total glycosides all increased with the increase of pH, showing a consistent change trend of solubility. The hydrophilicity was also suitable and similar. Overall, three kinds of total glycosides showed good stability, but strong hygroscopicity. The degree of hygroscopicity was as follows: total glycosides of Gen-tianae Macrophyllae Radix > total glycosides of Corni Fructus > total glycosides of Panacis Majoris Rhizome. Therefore, the hygroscopi-city needed to be considered in the preparation of the component-based Chinese medicine of Qinqi Fengshi Fang. The excipients and packaging materials can be properly selected to reduce the hygroscopicity of the preparation. This study provides a reference for the dosage form design of the component-based Chinese medicine of Qinqi Fengshi Fang.


Assuntos
Cornus , Medicamentos de Ervas Chinesas , Medicina Tradicional do Leste Asiático , Glicosídeos , Rizoma
4.
Huan Jing Ke Xue ; 41(6): 2812-2821, 2020 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-32608798

RESUMO

To investigate the potential interference of dead bacteria DNA on the analysis of antibiotic resistance genes (ARGs) and microbial communities in anaerobic digestion sludge, three different types of anaerobic digestion sludge were treated with propidium monoazide (PMA) in this study, and the results of subsequent ARGs and microbial community analysis with the interference of dead cell DNA blocked by PMA was compared to that without PMA treatment. It was found that after PMA treatment, the absolute abundance of the ARGs in the anaerobic digestion sludge from self-anaerobic digestion system of excess sludge and from high solid sludge anaerobic digestion system decreased by 41%-86%, and 74%-98%, respectively. ARGs abundance in the sludge from the anaerobic digestion system with sludge hydrolyzate as a substrate and anaerobic granular sludge as inoculum after 15 days of digestion considerably decreased with PMA treatment. However, its ARGs abundance still declined by up to 34%. PMA treatment influenced the analysis of microbial community of the three types of anaerobic digestion sludge to different degrees, in which the influence was the highest on the community structure analysis of the sludge from high solid anaerobic digestion system. The results of the correlation analysis between the ARGs abundance and the microbial community composition were completely different in the case with PMA treatment and without PMA treatment. This study proved the potential interference of dead cell DNA on the analysis of ARGs and microbial community in anaerobic digestion sludge. PMA pretreatment could achieve a more accurate analysis of the microbial community and ARGs characteristics in anaerobic digestion sludge.


Assuntos
Microbiota/efeitos dos fármacos , Esgotos , Anaerobiose , Antibacterianos/farmacologia , Resistência Microbiana a Medicamentos , Genes Bacterianos/efeitos dos fármacos
5.
Vector Borne Zoonotic Dis ; 20(10): 755-762, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32679008

RESUMO

As one of the important tick-borne zoonotic pathogens, Anaplasma has both veterinary and public health significance. Here, we performed a survey of Anaplasma infection in the goats from a farm in Beijing, China, and found 44.6% (41/92) were infected with Anaplasma capra, and 22.8% (21/92) were infected with Anaplasma sp. This Anaplasma sp. bacterium was close to a recently emerging Anaplasma platys strain based on gltA and groEL gene phylogenetic analysis. As to further understand the characteristics of Anaplasma sp., we raised a couple of positive goats (n = 2) in the laboratory with tick-free settings. We observed inappetence, vomiting, high fever, and weakness of limbs in the goat's offspring (n = 3). In addition, the blood samples from all offspring were all positive of this Anaplasma spp. We did not see any intracellular morulae in neutrophils, monocytes, and erythrocytes, but we identified some in the platelets of the blood smears from the positive goats by light microscopy. We named it A. platys-like and suggested it may infect platelets and be transmitted vertically through the placenta of goats. These findings deserve further evaluation.

6.
Zhongguo Zhong Yao Za Zhi ; 45(1): 106-112, 2020 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-32237418

RESUMO

To explore the permeation mechanism of micro-molecule medicinal ingredients of water extract of tradition Chinese medicine(TCM) in membrane separation process. With phenolic acid components as the model solute, five phenolic acids with similar molecular weight and structure, namely gallic acid, protocatechuate acid, 4-hydroxybenzoic acid, 3-hydroxybenzoic acid and salicylic acid, were selected in the PES membrane separation experiments. With the relative flux and the transmission rate as indexes, the scanning electron microscopy(SEM) and the electrochemical impedance spectroscopy(EIS) were used to analyze the permeation mechanism of different phenolic acid components. The results showed phenolic acids with similar molecular weight had different permeation behaviors, with decreased relative flux and increased solute permeation with the increase of solute concentration. According to the permeation behavior analyzed by the molecular structure of solute, the transmission rate of phenolic acids increased with the increase of the number of hydroxyl, and the order of substituent positions of phenolic acids based on the permeation rate as follows: para-substituted > meta-substitution > ortho-substitution. Electrochemical impedance spectroscopy reflected the role of charge repulsion in the membrane process; that is to say, the greater the resistance is, the less the solute permeation is. Therefore, the permeation phenomenon of the phenolic acid components in the PES membrane is not only the result of simple sieving mechanisms, but also has the effects of steric hindrance and charge repulsion during the membrane process.


Assuntos
Medicamentos de Ervas Chinesas/análise , Hidroxibenzoatos/isolamento & purificação , Membranas Artificiais , Medicina Tradicional Chinesa , Estrutura Molecular , Peso Molecular
7.
J Med Entomol ; 57(4): 1270-1276, 2020 07 04.
Artigo em Inglês | MEDLINE | ID: mdl-32053722

RESUMO

The importance of ticks in veterinary and medical science has received much attention. The dominant tick species in northeastern China, Ixodes persulcatus tick can transmit various pathogens to humans and animals and there are some studies on the microbiome composition of this tick. Our study characterized the bacterial communities in I. persulcatus by 16S amplicon pyrosequencing and described the differences of microorganisms in male and female tick and assessed the variation of microorganisms in the development stages in northeastern China. We mainly found the following bacteria genera: Pseudomonas (Pseudomonadales: Pseudomonadaceae), Citrobacter (Enterobacteriales: Enterobacteriaceae), and Rickettsia (Rickettsiales: Rickettsiaceae). Rickettsia is common and harmful pathogen transmitted by ticks. Meanwhile, we found there were differences between male and female tick of microbiomes, and the diversity of microbiome increased from engorged female ticks to eggs, but decreased when the eggs were molting into larvae. Our data showed that male ticks exhibited greater microbial diversity than female I. persulcatus tick and larvae presented with a different bacterial community compared to engorged female tick and hatched eggs. These findings may be useful for further understanding the interaction between I. persulcatus and microbiome biology.

8.
Infect Immun ; 88(4)2020 03 23.
Artigo em Inglês | MEDLINE | ID: mdl-31907196

RESUMO

Spotted fever group rickettsia (SFGR) can cause mild to fatal illness. The early interaction between the host and rickettsia in skin is largely unknown, and the pathogenesis of severe rickettsiosis remains an important topic. A surveillance of SFGR infection by PCR of blood and skin biopsy specimens followed by sequencing and immunohistochemical (IHC) detection was performed on patients with a recent tick bite between 2013 and 2016. Humoral and cutaneous immunoprofiles were evaluated in different SFGR cases by serum cytokine and chemokine detection, skin IHC staining, and transcriptome sequencing (RNA-seq). A total of 111 SFGR cases were identified, including 79 "Candidatus Rickettsia tarasevichiae," 22 Rickettsia raoultii, 8 Rickettsia sibirica, and 2 Rickettsia heilongjiangensis cases. The sensitivity to detect SFGR in skin biopsy specimens (9/24, 37.5%) was significantly higher than that in blood samples (105/2,671, 3.9%) (P < 0.05). As early as 1 day after the tick bite, rickettsiae could be detected in the skin. R. sibirica infection was more severe than "Ca Rickettsia" and R. raoultii infections. Increased levels of serum interleukin-18 (IL-18), IP10, and monokine induced by gamma interferon (MIG) and decreased levels of IL-2 were observed in febrile patients infected with R. sibirica compared to those infected with "Ca Rickettsia." RNA-seq and IHC staining could not discriminate between SFGR-infected and uninfected tick bite skin lesions. However, the type I interferon (IFN) response was differently expressed between R. sibirica and R. raoultii infections at the cutaneous interface. It is concluded that skin biopsy specimens were more reliable for the detection of SFGR infection in human patients although the immunoprofile may be complicated by immunomodulators induced by the tick bite.


Assuntos
Fatores Imunológicos/análise , Rickettsia/crescimento & desenvolvimento , Pele/patologia , Rickettsiose do Grupo da Febre Maculosa/patologia , Picadas de Carrapatos/complicações , Biópsia , Citocinas/sangue , Perfilação da Expressão Gênica , Humanos , Imuno-Histoquímica , Pele/imunologia , Pele/microbiologia , Rickettsiose do Grupo da Febre Maculosa/imunologia , Rickettsiose do Grupo da Febre Maculosa/microbiologia
9.
J Med Virol ; 92(8): 1085-1092, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-31850595

RESUMO

Enterovirus A71 (EV-A71) infection is known to cause hand, foot, and mouth disease (HFMD). Last year, an inactivated EV-A71 whole virus vaccine was used to prevent this disease in Yunnan, China. To obtain a viral genetic background for evaluating vaccine protection and monitor the adaptive evolution of the virus after the vaccination, a 5-year molecular epidemiology survey was performed before the vaccination. Twenty-six EV-A71 strains were separated from 561 stool specimens of patients with serious HFMD. The whole-genomic sequences of these strains were sequenced. Phylogenetic trees were constructed, and the mutation spectra were analyzed based on these viral sequences. There was no obvious mutation for the circular EV-A71 strains of the same year. Pathogenic EV-A71 strains may arise from a "subgroup" randomly each year. Whole-genomic analyses showed that a hotspot nonsynonymous substitution potentially affecting the immunogenicity of vaccines was found in the 2A gene, but not in genes of the viral capsid proteins, and the genetic diversity of whole viral genomes associated with the incidence of HFMD. Therefore, it will be valuable to monitor the genome-wide changes of EV-A71 to detect the adaptive mutations affecting immunogenicity or perform investigations using genetic diversity as a parameter.

11.
World J Clin Cases ; 7(15): 2022-2037, 2019 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-31423434

RESUMO

BACKGROUND: Noninvasive biomarkers have been developed to predict hepatitis B virus (HBV) related fibrosis owing to the significant limitations of liver biopsy. Both serum biomarkers and imaging techniques have shown promising results and may improve the evaluation of liver fibrosis. However, most of the previous studies focused on the diagnostic effects of various imaging techniques on fibrosis in all chronic liver diseases. AIM: To compare the performance of common imaging methods and serum biomarkers for prediction of significant fibrosis caused only by HBV infection. METHODS: A systematic review was conducted on the records available in PubMed, EMBASE, and the Cochrane Library electronic databases until December 2018. We systematically assessed the effectiveness of two serum biomarkers and three imagine techniques in predicting significant fibrosis solely caused by HBV infection. The serum biomarkers included aspartate aminotransferase-to-platelet ratio index (APRI) and fibrosis index based on the 4 factors (FIB-4). The three imaging techniques included acoustic radiation force impulse (ARFI), FibroScan, and magnetic resonance elastography (MRE). Three parameters, the area under the summary receiver operating characteristic curve (AUSROC), the summary diagnostic odds ratio, and the summary sensitivity and specificity, were used to examine the accuracy of all tests for liver fibrosis. RESULTS: Out of 2831 articles evaluated for eligibility, 204 satisfied the predetermined inclusion criteria for this current meta-analysis. Eventually, our final data contained 81 studies. The AUSROCs of serum biomarkers of APRI and FIB-4 were both 0.75. For imaging techniques (ARFI, FibroScan, and MRE), the areas were 0.89, 0.83, and 0.97, respectively. The heterogeneities of ARFI and FibroScan were statistically significant (I 2 > 50%). The publication bias was not observed in any of the serum biomarkers or imaging methods. CONCLUSION: These five methods have attained an acceptable level of diagnostic accuracy. Imaging techniques, MRE in particular, demonstrate significant advantages in accurately predicting HBV-related significant fibrosis, while serum biomarkers are admissible methods.

12.
Ying Yong Sheng Tai Xue Bao ; 30(6): 2093-2100, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31257784

RESUMO

In this study, we evaluated the effectiveness of SrCl2·6H2O to mark otoliths of juvenile common carp (Cyprinus carpio). The carp fish were immersed with four concentrations of the Sr compound (i.e., 0, 4, 8, 12 mg·L-1) for 2 d to determine the essential concentration for immersing. Then, the carp fish were immersed in 8 mg·L-1 of the Sr compound for five different immersing times (i.e., 1, 2, 3, 4, 5 d) to establish the essential immersing time. Results from electron microprobe analysis (EPMA) showed that otolith Sr marks were very obvious in all C. carpio fish of the aforementioned Sr immersed group with high ratio of Sr/Ca concentration and red color (high Sr level) ring map, compared to the control group with low ratio of Sr/Ca concentration and basic blue color (low Sr level) map. We obtained a 100% mark-success rate of otolith Sr marking in juvenile C. carpio. No significant difference were observed on the average body length, mass and mortality between the immersed group and the control group, suggesting that Sr marking had no negative impact on fish. As clear and complete otolith Sr marked rings occurred at concentration of 8 mg·L-1 or more, and immersing time of 2 d or more for the Sr compound, 8 mg·L-1 and 2 d were suggested as the essential immersing Sr concentration and immersing time, respectively. Our results demonstrated the strong feasibility of otolith Sr marking for juvenile C. carpio.


Assuntos
Carpas/metabolismo , Membrana dos Otólitos/metabolismo , Estrôncio/metabolismo , Animais
13.
J Nanosci Nanotechnol ; 19(11): 7269-7277, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31039885

RESUMO

Pillared graphene composite (GP) is prepared by in situ polymerization and subsequent carbonization of graphene oxide (GO) and polyaniline (PANI) precursors. The interlayer spacing of GO layer can reach 1.418 nm with 200% increase compared with the original spacing of 0.706 nm by the intercalation of aniline monomer through π-π conjugate and electrostatic interactions. After carbonization, the graphene composite is reinforced by the intercalated PANI-converted carbon pillars and also has a nitrogen-doped level of ca. 4.49 atom%. Electrochemical characterization studies show that the GP composite exhibits a high reversible capacity of 653 mAh g-1 at a current density of 100 mA g-1 and an excellent rate capability (343 mAh g-1 at a current density of 1 A g-1), which are superior to graphene owing to the unique pillared and the nitrogen-doped structure.

14.
EBioMedicine ; 43: 317-324, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31003930

RESUMO

BACKGROUND: A tick-borne segmented RNA virus called Jingmen tick virus (JMTV) was recently identified, variants of which were detected in a non-human primate host and fatal patients with Crimean-Congo haemorrhagic fever. We investigated its infectivity and pathogenicity for humans. METHODS: We obtained skin-biopsy, blood and serum samples from patients with tick bites, and used high-throughput sequencing, in situ hybridisation, and serologic testing to diagnose and ascertain the cases of JMTV infection. FINDINGS: A JMTV strain was isolated from the tick Amblyomma javanense into an embryo-derived tick cell line. We obtained sustained passage of JMTV, and revealed that it was able to accumulate in salivary glands of experimentally infected ticks. Four JMTV-infected patients were identified by high-throughput sequencing of skin biopsies and blood samples. The virus replication in skin tissue was visualised by in situ hybridisation. The four patients all had an itchy or painful eschar at the site of tick bite, with or without lymphadenopathy. Immunohistochemical examination revealed remarkable local inflammation manifested as infiltration by neutrophils. Eight patients were identified by serological testing and showed more severe clinical manifestations. Two Ixodes persulcatus ticks detached from patients were positive for JMTV. All JMTV strains identified in this study formed a well-supported sub-lineage, distinct from those previously reported in China. Interpretation The public significance of JMTV should be highly concerning due to its potential pathogenicity for humans and efficient transmission by potential ticks. FUND: China Natural Science Foundation, State Key Research Development Programme, and United Kingdom Biotechnology and Biological Sciences Research Council.


Assuntos
Doenças Transmissíveis Emergentes/epidemiologia , Doenças Transmissíveis Emergentes/virologia , Infecções por Flavivirus/epidemiologia , Infecções por Flavivirus/virologia , Flavivirus , Biomarcadores , China , Doenças Transmissíveis Emergentes/diagnóstico , Doenças Transmissíveis Emergentes/transmissão , Flavivirus/classificação , Flavivirus/genética , Infecções por Flavivirus/diagnóstico , Infecções por Flavivirus/transmissão , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Imuno-Histoquímica , Hibridização In Situ , Filogenia , Vigilância em Saúde Pública , RNA Viral , Estudos Retrospectivos , Testes Sorológicos , Pele/patologia , Picadas de Carrapatos
15.
Eval Program Plann ; 73: 176-186, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30665124

RESUMO

Researchers worldwide are increasingly reporting the societal impact of their research as part of national research productivity assessments. However, the challenges they encounter in developing their impact case studies against specified government assessment criteria and how pitfalls can be mitigated are not reported. This paper examines the key steps taken to develop an Aboriginal Family Wellbeing (FWB) empowerment research impact case study in the context of an Australian Research Council (ARC) pilot research impact assessment exercise and the challenges involved in applying the ARC criteria. The requirement that researchers demonstrate how their institutions support them to conduct impactful research has the potential to create supportive environments for researchers to be more responsive to the needs of users outside academia. However, the 15-year reference period for the associated research underpinning the reported impact and the focus on researcher's current institutional affiliation constitute potential constraints to demonstrating the true impact of research. For researchers working with Indigenous people, relationships that build over long periods of time, irrespective of university affiliation, are critical to conducting impactful research. A more open-ended time-frame, with no institutional restrictions for the 'associated research' provides the best opportunity to demonstrate the true benefits of research not only for Indigenous people but for Australian society more broadly.


Assuntos
Pesquisa Participativa Baseada na Comunidade/métodos , Saúde da Família/etnologia , Grupo com Ancestrais Oceânicos , Avaliação de Programas e Projetos de Saúde/métodos , Austrália , Pesquisa Participativa Baseada na Comunidade/normas , Relações Comunidade-Instituição , Humanos , Projetos Piloto , Poder Psicológico , Avaliação de Programas e Projetos de Saúde/normas
17.
Parasitology ; 146(2): 161-167, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30066666

RESUMO

Northeastern China is a region of high tick abundance, multiple tick-borne pathogens and likely human infections. The spectrum of diseases caused by tick-borne pathogens has not been objectively evaluated in this region for clinical management and for comparison with other regions globally where tick-transmitted diseases are common. Based on clinical symptoms, PCR, indirect immunofluorescent assay and (or) blood smear, we identified and described tick-borne diseases from patients with recent tick bite seen at Mudanjiang Forestry Central Hospital. From May 2010 to September 2011, 42% (75/180) of patients were diagnosed with a specific tick-borne disease, including Lyme borreliosis, tick-borne encephalitis, human granulocytic anaplasmosis, human babesiosis and spotted fever group rickettsiosis. When we compared clinical and laboratory features to identify factors that might discriminate tick-transmitted infections from those lacking that evidence, we revealed that erythema migrans and neurological manifestations were statistically significantly differently presented between those with and without documented aetiologies (P < 0.001, P = 0.003). Twelve patients (6.7%, 12/180) were co-infected with two tick-borne pathogens. We demonstrated the poor ability of clinicians to identify the specific tick-borne disease. In addition, it is necessary to develop specific laboratory assays for optimal diagnosis of tick-borne diseases.


Assuntos
Doenças Transmitidas por Carrapatos/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Anaplasmose/sangue , Anaplasmose/diagnóstico , Anaplasmose/epidemiologia , Babesiose/sangue , Babesiose/diagnóstico , Babesiose/epidemiologia , Criança , Pré-Escolar , China/epidemiologia , Encefalite Transmitida por Carrapatos/sangue , Encefalite Transmitida por Carrapatos/diagnóstico , Encefalite Transmitida por Carrapatos/epidemiologia , Feminino , Humanos , Lactente , Doença de Lyme/sangue , Doença de Lyme/diagnóstico , Doença de Lyme/epidemiologia , Masculino , Pessoa de Meia-Idade , Prevalência , Rickettsiose do Grupo da Febre Maculosa/sangue , Rickettsiose do Grupo da Febre Maculosa/diagnóstico , Rickettsiose do Grupo da Febre Maculosa/epidemiologia , Picadas de Carrapatos/complicações , Picadas de Carrapatos/patologia , Doenças Transmitidas por Carrapatos/sangue , Doenças Transmitidas por Carrapatos/diagnóstico , Adulto Jovem
18.
Zhongguo Zhong Yao Za Zhi ; 43(19): 3876-3883, 2018 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-30453712

RESUMO

In order to analyze the law of membrane permeation of different alkaloids, seven traditional Chinese medicine alkaloids with different parent nucleus and substituent structures, including berberine, palmatine, sinomenine, matrine, oxymatrine, sophoridine, and tetrandrine, were prepared into the simulated solution with same molar concentration, and the membrane penetrating experiments with membrane RC1K and membrane RC5K were carried out. The dynamic transmittance, the total transmittance and the total adsorption rate of each substance were measured, and the scanning electron microscopy (SEM) images of the membrane surface before and after the membrane experiment were considered to predict and analyze the reason of differences in dynamic transmittance of different alkaloids. The results showed that there were significant differences in the dynamic transmittance of the chemical constituents of different alkaloids during penetrating the two membranes. The contamination degree on the surface of the membrane material was also different. The transmittance of the same compound through the RC5K membrane was larger than that through RC1K membrane. Within a certain range, the smaller the pore size of the membrane, the better the selective screening effect on the chemical constituents of traditional Chinese medicine. All the membrane surfaces were less polluted. The difference in transmittance between different substances on the same membrane showed a positive correlation with the difference in structural complexity, providing an experimental basis for the surface modification design in contamination control of membrane materials. In the design of membrane modified material, the surface properties of the membrane can be improved by grafting different polar groups, thereby changing the adsorption characteristics of the membrane surface. The pore size was designed accordingly to achieve the high transmittance and low pollution of the corresponding compounds.


Assuntos
Alcaloides de Berberina/química , Medicamentos de Ervas Chinesas/química , Medicina Tradicional Chinesa , Permeabilidade
19.
Fitoterapia ; 130: 265-271, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30243779

RESUMO

Gracilistones A (1) and B (2), two new eudesmane-type sesquiterpenoids with an unusual tetrahydrofuran-fused 6/6/5 tricyclic ring system, were obtained from Acanthopanax gracilistylus under the guidance of LC-MS investigation. Their structures and absolute configurations were assigned by extensive spectroscopic analyses and quantum calculation methods. Compounds 1 and 2 showed potent inhibitory activity against LPS-induced nitric oxide production in RAW 264.7 macrophages, compared with the positive control L-NMMA. In addition, compounds 1 and 2 were also evaluated for their antioxidant (DPPH• and ABTS•+) and xanthine oxidase (XO) inhibitory activities, and they exhibited weak inhibitory effects at 100 µM.


Assuntos
Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Eleutherococcus/química , Sesquiterpenos de Eudesmano/farmacologia , Animais , Anti-Inflamatórios/isolamento & purificação , Antioxidantes/isolamento & purificação , China , Cromatografia Líquida , Furanos , Espectrometria de Massas , Camundongos , Estrutura Molecular , Óxido Nítrico/metabolismo , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Raízes de Plantas/química , Células RAW 264.7 , Sesquiterpenos de Eudesmano/isolamento & purificação
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