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1.
PLoS One ; 14(9): e0222003, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31483841

RESUMO

In this study, a new type of N2-inhibitor-water mist (NIWM) technology was proposed to resolve the problem of fire prevention and extinguishing in the goaf of coal mine. The corresponding equipment was designed and manufactured. Under the condition that both gas pressure and liquid pressure were 0.5-2MPa, the NIWM equipment produced the water mist with Sauter mean diameter (SMD) range of 166-265µm. The experimental results of the operating parameters of NIWM equipment were in agreement with the theoretical derivation. The theory of two-phase flow atomisation can be used as theoretical guide for this technology. After that, on the basis of the NIWM equipment, the experiments of inhibiting low temperature (30-100°C) oxidation and extinguishing high temperature combustion of large dosage of coal sample were carried out. Water mist with SMD = 188µm had good diffusivity in the container. The inhibiting effect of N2-inhibitor-water mist on low temperature oxidation of coal was obviously greater than that of single material. N2-water mist extinguished the burning coal completely in 20 minutes. The addition of water mist solved the shortcoming of poor cooling effect of N2. In different stages of coal-oxygen reaction, N2, inhibitor and water mist play very different role in controlling the process of coal-oxygen reaction, which was not simple accumulation of the three. The combination of N2, inhibitor and water mist should be determined according to the state of the coal mine goaf fire. On the basis of the research conclusions, the onsite arrangement diagram of the NIWM fire prevention and extinguishing equipment in the goaf was designed. The research results proved the feasibility and effectiveness of this technology, and it is of great significance to the prevention and control of coal spontaneous combustion in goaf.

2.
Artigo em Inglês | MEDLINE | ID: mdl-31479802

RESUMO

OBJECTIVES: The clinical effect of 5-aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) may be correlated with the degree of dysplasia of cancer tissues, but much is still unknown regarding the differences in its effectiveness, especially in oral cancer and precancerous lesions. The aim of this study is to compare the effects of ALA-PDT on a human oral precancerous cell line (DOK) and an oral squamous cell carcinoma cell line (CAL-27). METHODS: First, we explored the dose- and time-dependent responses of DOK and CAL-27 cells to ALA-PDT. DOK and CAL-27 cells were incubated with various concentrations of ALA (from 0.25 to 2 mM), followed by PDT using laser irradiation at 635 nm. The resulting photocytotoxicity was assessed in both cell lines using MTT assays. Further, apoptosis was assessed using flow cytometry, reactive oxygen species (ROS) generation was evaluated with 2,7-dichlorofluorescein diacetate (DCFH2-DA), and the response to treatment was examined via RT-qPCR and Western blotting to measure the mRNA and protein expression levels of matrix metallopeptidase 2 (MMP-2) and MMP-9. RESULTS: ALA-PDT inhibited the proliferation of DOK and CAL-27 cells in a dose- and time-dependent manner. Dose-effect and inhibition-time relationships were also found. The rates of DOK and CAL-27 cell apoptosis when the ALA dose was 1 mM were 30.66 ± 3.10 % and 75.40 ± 1.29 %, respectively (P < 0.01). Following PDT, compared with DOK cells, the ROS level in CAL-27 cells was significantly increased and was correlated with an increase in the ALA concentration. Mechanistically, both the mRNA and protein expression levels of MMP-2 and MMP-9 were found to be regulated in both cell types after ALA-PDT. CONCLUSION: ALA-PDT effectively killed DOK and CAL-27 cells in a dose- and time-dependent manner in vitro. However, under the same conditions, the susceptibilities of these cell lines to ALA-PDT were different. Further studies are necessary to confirm whether this difference is present in clinical oral cancer and precancerous lesions.

4.
Br J Neurosurg ; : 1-4, 2019 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-31514549

RESUMO

Purpose: To report a rare complication that Onyx gel blocked the MCA trunk and branches unexpectedly during AVM embolism and our strategy to rescue. Material and methods: A 16 years old otherwise healthy girl hold a left side Spetzler - Martin grade III fronto-temporal AVM, during embolization, the L-MCA and its branches were blocked by Onyx completely, the patient was transferred to the operating room to extract the Onyx gel immediately. Result: After totally 10 arterotomies, all the Onyx gel were removed. 8 hours after occlusion, all arteries were then seen to pulsate. Conclusion: Iatrogenic MCA full-length acute occlusion is a rare and severe complication during AVM embolism. Carefully identify the feeding arteries, micro-catheter angiography before Onyx gel injection and balloon-assisted embolism could probably prevent it. Surgical operation to extract onyx gel and re-canalize MCA was recommended, AVM should be resect if possible.

5.
Mol Cancer ; 18(1): 133, 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31481066

RESUMO

BACKGROUND: Circular RNAs (circRNAs) represent a subclass of regulatory RNAs that have been shown to have significant regulatory roles in cancer progression. However, the biological functions of circRNAs in bladder cancer (BCa) are largely unknown. METHODS: Cell invasion models were established, and invasion-related circRNAs were detected by qPCR. Using above method, circ-ZKSCAN1 was picked out for further study. Circ-ZKSCAN1 expression and survival analyses were performed through qPCR. The survival curves were generated by the Kaplan-Meier method, and the log-rank test was used to assess the significance. Cell proliferation, migration and invasion were examined to investigate the function of circ-ZKSCAN1. Tumorigenesis in nude mice was assessed to determine the effect of circ-ZKSCAN1 in bladder cancer. Biotin-coupled probe pull-down assays, FISH and luciferase reporter assays were conducted to confirm the relationship between circ-ZKSCAN1 and microRNA. RNA-seq revealed different molecular changes in downstream genes. RESULTS: Here, we found that circ-ZKSCAN1 was downregulated in BCa tissues and cell lines. Circ-ZKSCAN1 levels were associated with survival, tumor grade, pathological T stage and tumor recurrence. Overexpressed circ-ZKSCAN1 inhibits cell proliferation, migration, invasion and metastasis in vitro and in vivo. Mechanistically, we demonstrated that circ-ZKSCAN1 upregulated p21 expression by sponging miR-1178-3p, which suppressed the aggressive biological behaviors in bladder cancer. CONCLUSIONS: These results reveal that Circ-ZKSCAN1 acts as a tumor suppressor via a novel circ-ZKSCAN1/miR-1178-3p/p21 axis, which have the important role in the proliferation, migration and invasion ablitities of BCa cells and provide a novel perspective on circRNAs in BCa progression.

6.
J Cardiopulm Rehabil Prev ; 39(5): 290-292, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31464884

RESUMO

The evidence base supporting cardiac rehabilitation is substantial and overwhelmingly supports its utilization for all qualified patients. However, important lines of inquiry remain and require attention. This commentary provides a model for cardiac rehabilitation centers that provide patient care to meaningfully contribute to our scientific understanding of this lifestyle intervention.

7.
Medicine (Baltimore) ; 98(33): e16879, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31415430

RESUMO

BACKGROUND: Optic atrophy (OPA) is a very tricky disorder. Presently, no effective management is available for this condition. Previous studies have reported that acupuncture may be effective for the treatment of OPA. However, its effectiveness is still inconclusive. Thus, this study will aim to assess the effectiveness and safety of acupuncture for OPA. METHODS: A comprehensive literature search for relevant studies will be performed from the databases of PUMBED, EMBASE, CINAHI, Cumulative Index to Nursing and Allied Health Literature, Allied and Complementary Medicine Database, Cochrane Library, Chinese Biomedical Literature Database, China National Knowledge Infrastructure, and other literature sources from inception up to the present. No language limitations will be applied to all literature searches. We will consider all randomized controlled trials (RCTs) and case-controlled trials (CCTs) for assessing the effectiveness and safety of acupuncture for OPA. The primary outcomes include the rates of vision improvement and visual field improvement. The secondary outcomes consist of the increased visual field average sensitivity, pattern visual evoked potential (PVEP) amplitude, and shortened PVEP latency, as well as any expected and unexpected adverse reactions. Risk of bias assessment will be performed by Cochrane risk of bias for RCTs and Newcastle-Ottawa Scale for CCTs. RESULTS: In this study, we will outline details of the aims and methods on the effectiveness and safety of acupuncture for the treatment of OPA. CONCLUSION: The results of this study will summarize the most current evidence of acupuncture for the treatment of patients with OPA. DISSEMINATION AND ETHICS: The results of this study are expected to be published on peer-reviewed journals. This is a literature-based study; therefore, no ethical approval is necessary. SYSTEMATIC REVIEW REGISTRATION: PROSPERO CRD42019135785.


Assuntos
Terapia por Acupuntura/métodos , Atrofia Óptica/terapia , Potenciais Evocados Visuais , Humanos , Revisão Sistemática como Assunto , Resultado do Tratamento
9.
Phytopathology ; 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31433275

RESUMO

The co-existence of cereal cyst nematode (CCN) species Heterodera avenae and H. filipjevi together with multiple pathotypes is a limiting factor for wheat production in China. Some of the known genes for resistance to CCN are not effective against both nematode species, which complicates breeding efforts to develop CCN-resistant wheat cultivars. Here we demonstrate that the CCN resistance of wheat cultivar Madsen to both Heterodera species is controlled by different genetic loci, both of which originated from Aegilops ventricosa. A new QTL, QCre-ma7D, was identified and localized in a 3.77 Mb genomic region on chromosome 7DL, which confers resistance to H. filipjevi. QCre-ma2A on chromosome 2AS corresponds to CCN resistance gene Cre5 and confers resistance to H. avenae. Three KASP markers, BS00150072, BS00021745, and BS00154302, were developed for molecular marker-assisted selection of QCre-ma7D and locally adapted wheat lines with resistance to both nematode species were developed. The identification of different loci underlying resistance to H. avenae and H. filipjevi and the development of adapted resistant entries will facilitate development of wheat cultivars that are resistant to these devastating nematodes in China.

10.
Trends Ecol Evol ; 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31422890

RESUMO

Our knowledge of host-associated microorganisms and their role in host functions is rapidly evolving. Stress-affected plants assemble beneficial microbes in their rhizosphere to maximize survival and growth. Similarly, insects have gut microbiomes that extend their functional repertoire in fighting stress. A strong microbial linkage between soil, plants, and pollinators is emerging and this can influence pollination services and overall ecosystem health. Yet, the nature of microbial interactions between different ecosystem components remains poorly understood. Here we highlight the acquisition pathways of beneficial microbes and their functions in protecting hosts against stress. By adopting a new 'eco-holobiont' approach, which explicitly incorporates biotic feedbacks, we can significantly expand our ecological understanding and better develop sustainable environmental management.

11.
Bull Environ Contam Toxicol ; 103(2): 336-341, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31297552

RESUMO

Phytoremediation experiments were carried out to assess the ability of different herbaceous buffer strips to absorb metals. Results revealed the Medicago sativa buffer strip had the best removal capacity for copper, lead, and cadmium in the runoff and seepage flow. Copper and lead content in M. sativa were significantly higher than in Lolium perenne and Poa pratensis (p < 0.05). Cadmium content in P. pratensis was significantly higher than in L. perenne and M. sativa(p < 0.05). Total accumulation of copper and lead in M. sativa buffer strips was 13.45 mg kg-1 and 1.01 mg kg-1, respectively. Total cadmium accumulation was approximately 0.50 mg kg-1 in both M. sativa and P. pratensis. Overall, results indicated that using M. sativa in the buffer strips was optimal for the remediation of copper, lead, and cadmium in sewage water.

12.
Biochim Biophys Acta Mol Basis Dis ; 1865(10): 2647-2656, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31319155

RESUMO

A tightly controlled activity of renin-angiotensin system (RAS) including renin, angiotensin-converting enzymes (ACEs), and angiotensin II (Ang II) receptors is critical not only for maintaining systemic hemodynamics and blood volume but also for controlling cell proliferation, differentiation, and tissue remodeling in target organs. ACE inhibitors or Ang II receptor type 1 (AT1R) blockers are widely used as first line drugs for the treatment of cardiovascular diseases that are caused by chronical activation of RAS. However, about 15% of patients using ACE inhibitors develop side effects in the skin and the underlying mechanisms have been poorly understood or even neglected. Herein we show an endogenous RAS in maintaining self-renewal and regeneration potential of epidermal stem cells (ESCs) thereby contributing to wound healing. Firstly, we found that ESCs may express ACE, and its members in wound edges were positively associated with wound healing in Captopril-treated rats. Secondly, we demonstrated that human ESCs had a functional RAS including ACE1, ACE2, Ang II, AT1R, and AT2R. ACE-Ang II axis maintains human ESC function via activation of both AT1R and AT2R, which are negatively regulated by each other. Ang II-induced activation of extracellular signal-regulated kinase (ERK) and signal transducers and activators of transcription (STAT)1 and STAT3 was mediated by the negative cross-talk between AT1R and AT2R in human ESCs. These results suggest that Ang II is a critical regulator of ESC function and ESC-mediated epidermal regeneration. Inappropriate interruption of Ang II-operated signaling may prejudice ESC function leading to impaired skin wound healing or even disease.

13.
J Agric Food Chem ; 67(26): 7348-7364, 2019 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-31180673

RESUMO

A chemical study on the peels of the cultivated edible mushroom Wolfiporia cocos led to the isolation and identification of 47 lanostane triterpenoids including 16 new compounds (1-16). The structures of the new compounds were determined by analysis of the NMR, MS, and electronic circular dichroism (ECD) data. Compounds 1 and 2 represent new members of the family of 4,5-secolanostane triterpenes. Compound 3 is a new aromatic lanostane triterpene with an unusual methyl rearrangement from C-10 to C-6. The absolute configurations of 1 and 8 were assigned by ECD spectra calculation. All compounds were evaluated for cytotoxicity (K562, SW480, and HepG2) and glucose-uptake-stimulating effects. Compounds 23, 25, 29, and 31 showed weak inhibition on the K562 cells with IC50 in the range of 25.7 to 68.2 µM, respectively. Compounds 21, 28, and 30 increased the glucose uptake in 3T3-L1 cells by 25%, 14%, and 50% at 5 µM, respectively. In addition, compounds 14, 23, 29, 35, and 43 showed insulin-sensitizing activity by increasing the insulin-stimulated glucose uptake at 2.5 µM in 3T3-L1 adipocytes. A preliminary structure-activity relationship analysis indicates that the 6/6/6/5 ring skeleton and the double bond between C-8 and C-9 are beneficial for the glucose-uptake-stimulating and insulin-sensitizing activities. Furthermore, the alkaline-insoluble fraction mainly containing compounds 22, 24, 28, and 31 were confirmed to have hypoglycemic and hypolipidemic activity on high-fat-diet-induced obese mice. This work confirms the potential of the peels' extracts of W. cocos as a functional food or dietary supplements.


Assuntos
Glucose/metabolismo , Extratos Vegetais/farmacologia , Triterpenos/farmacologia , Wolfiporia/química , Células 3T3-L1 , Adipócitos/efeitos dos fármacos , Adipócitos/metabolismo , Animais , Transporte Biológico/efeitos dos fármacos , Hipoglicemiantes/química , Hipoglicemiantes/isolamento & purificação , Hipoglicemiantes/farmacologia , Insulina/metabolismo , Camundongos , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Triterpenos/química , Triterpenos/isolamento & purificação
14.
J Cell Mol Med ; 23(8): 5642-5653, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31199066

RESUMO

This study aimed to examine miR-140 expression in clinical samples from tuberculosis (TB) patients and to explore the molecular mechanisms of miR-140 in host-bacterial interactions during Mycobacterium tuberculosis (M tb) infections. The miR-140 expression and relevant mRNA expression were detected by quantitative real-time PCR (qRT-PCR); the protein expression levels were analysed by ELISA and western blot; M tb survival was measured by colony formation unit assay; potential interactions between miR-140 and the 3' untranslated region (UTR) of tumour necrosis factor receptor-associated factor 6 (TRAF6) was confirmed by luciferase reporter assay. MiR-140 was up-regulated in the human peripheral blood mononuclear cells (PBMCs) from TB patients and in THP-1 and U937 cells with M tb infection. Overexpression of miR-140 promoted M tb survival; on the other hand, miR-140 knockdown attenuated M tb survival. The pro-inflammatory cytokines including interleukin 6, tumour necrosis-α, interleukin-1ß and interferon-γ were enhanced by M tb infection in THP-1 and U937 cells. MiR-140 overexpression reduced these pro-inflammatory cytokines levels in THP-1 and U937 cells with M tb infection; while knockdown of miR-140 exerted the opposite actions. TRAF6 was identified to be a downstream target of miR-140 and was negatively modulated by miR-140. TRAF6 overexpression increased the pro-inflammatory cytokines levels and partially restored the suppressive effects of miR-140 overexpression on pro-inflammatory cytokines levels in THP-1 and U937 cells with M tb infection. In conclusion, our results implied that miR-140 promoted M tb survival and reduced the pro-inflammatory cytokines levels in macrophages with M tb infection partially via modulating TRAF6 expression.

15.
Plant Physiol Biochem ; 141: 343-352, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31207495

RESUMO

Plant U-box (PUB) E3 ubiquitin ligases play crucial roles in the plant response to abiotic stress and the phytohormone abscisic acid (ABA) signaling, but little is known about them in bryophytes. Here, a representative U-box armadillo repeat (PUB-ARM) ubiquitin E3 ligase from Antarctic moss Pohlia nutans (PnSAG1), was explored for its role in abiotic stress response in Arabidopsis thaliana and Physcomitrella patens. The expression of PnSAG1 was rapidly induced by exogenous abscisic acid (ABA), salt, cold and drought stresses. PnSAG1 was localized to the cytoplasm and showed E3 ubiquitin ligase activity by in vitro ubiquitination assay. The PnSAG1-overexpressing Arabidopsis enhanced the sensitivity with respect to ABA and salt stress during seed germination and early root growth. Similarly, heterogeneous overexpression of PnSAG1 in P. patens was more sensitive to the salinity and ABA in their gametophyte growth. The analysis by RT-qPCR revealed that the expression of salt stress/ABA-related genes were downregulated in PnSAG1-overexpressing plants after salt treatment. Taken together, our results indicated that PnSAG1 plays a negative role in plant response to ABA and salt stress.


Assuntos
Ácido Abscísico/farmacologia , Arabidopsis/fisiologia , Briófitas/enzimologia , Bryopsida/fisiologia , Estresse Salino , Ubiquitina-Proteína Ligases/genética , Regiões Antárticas , Arabidopsis/genética , Briófitas/genética , Bryopsida/genética , Biologia Computacional , Secas , Regulação da Expressão Gênica de Plantas , Células Germinativas Vegetais/metabolismo , Germinação , Raízes de Plantas/crescimento & desenvolvimento , Plantas Geneticamente Modificadas/fisiologia , Transdução de Sinais
16.
Sci China Life Sci ; 62(8): 1087-1095, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31209796

RESUMO

Filamentous fungi are excellent sources for the production of a group of bioactive small molecules which are often called secondary metabolites (SMs). The advanced genome sequencing technology combined with bioinformatics analysis reveals a large number of unexplored biosynthetic gene clusters (BGCs) in the fungal genomes. To unlock this fungal SM treasure, many approaches including heterologous expression are being developed and efficient cloning of the BGCs is a crucial step to do this. Here, we present an efficient strategy for the direct cloning of fungal BGCs. This strategy consisted of Splicing by Overlapping Extension (SOE)-PCR and yeast assembly in vivo. By testing 14 BGCs DNA fragments ranging from 7 kb to 52 kb, the average positive rate was over 80%. The maximal insertion size for fungal BGC assembly was 52 kb. Those constructs could be used conveniently for the heterologous expression leading to the discovery of novel natural products. Thus, our results provide an efficient and quick method for the low cost direct cloning of fungal BGCs.

17.
Planta Med ; 85(13): 1088-1097, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31216579

RESUMO

As part of our search for new cytotoxic and antimicrobial natural products from endolichenic fungi, 19 compounds including 1 new 10-member lactone (2: ), 1 new polyacetylene glycoside (3: ), 1 new brasilane-type sesquiterpenoid glycoside (4: ), and 2 isobenzofuran-1(3H)-one derivatives (5: and 6: ) were isolated from the solid culture of the endolichenic fungus Hypoxylon fuscum. Their structures were unambiguously elucidated by NMR spectroscopic data, MS, ECD (electronic circular dichroism) calculation, and chemical methods. The cytotoxic effects on K562, SW480, and HEPG2 cell lines and the antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Candida albicans were assessed. Compounds 1, 2: , and 5: exhibited moderate cytotoxicity against K562, SW480, and HEPG2 cell lines while compounds 1, 9: , and 11: displayed weak antibacterial activity against S. aureus.


Assuntos
Citotoxinas/isolamento & purificação , Xylariales/metabolismo , Anti-Infecciosos/isolamento & purificação , Anti-Infecciosos/farmacologia , Bacillus subtilis/efeitos dos fármacos , Candida albicans/efeitos dos fármacos , Linhagem Celular Tumoral/efeitos dos fármacos , Dicroísmo Circular , Citotoxinas/farmacologia , Escherichia coli/efeitos dos fármacos , Células Hep G2/efeitos dos fármacos , Humanos , Células K562/efeitos dos fármacos , Espectroscopia de Ressonância Magnética , Staphylococcus aureus/efeitos dos fármacos , Xylariales/química
18.
ACS Appl Mater Interfaces ; 11(22): 19986-19993, 2019 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-31083896

RESUMO

For lithium-sulfur batteries (LSBs), the dissolution of lithium polysulfide and the consequent "shuttle effect" remain major obstacles for their practical applications. In this study, we designed a new cathode material comprising MoSe2/graphene to selectively adsorb polysulfides on the selenium edges and thus to mitigate their dissolution. More specifically, few-layered MoSe2 was first grown on nitrogen-doped reduced graphene oxide (N-rGO) using the chemical vapor deposition method and then infiltrated with sulfur as the cathode for LSBs. An initial capacity of 1028 mA h g-1 was achieved for S/MoSe2/N-rGO at 0.2 C, higher than 981 and 405.1 mA h g-1 for pure graphene and sulfur, respectively, along with enhanced cycling durability and rate capability. Moreover, the density functional theory simulation, in addition to the experimental adsorption test, X-ray photoelectron spectroscopy analysis, and transmission electron microscopy technique, reveals the dual roles that MoSe2 plays in improving the performance of LSBs by functioning as the binding sites for lithium polysulfides and as the platform that enables fast Li-ion diffusion by reducing its diffusion barrier. The reported finding suggests that the transition-metal selenides could be an efficient alternative material as the cathode for LSBs.

19.
Sci Rep ; 9(1): 6892, 2019 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-31053834

RESUMO

Some microbes enhance stress tolerance in plants by minimizing plant ethylene levels via degradation of its immediate precursor, 1-aminocyclopropane-1-carboxylate (ACC), in the rhizosphere. In return, ACC is used by these microbes as a source of nitrogen. This mutualistic relationship between plants and microbes may be used to promote soil properties in stressful environments. In this study, we tested the hypothesis that amendments of ACC in soils reshape the structure of soil microbiome and alleviate the negative impacts of salinity on soil properties. We treated non-saline and artificially-developed saline soils with ACC in different concentrations for 14 days. The structure of soil microbiome, soil microbial properties and productivity were examined. Our results revealed that microbial composition of bacteria, archaea and fungi in saline soils was affected by ACC amendments; whereas community composition in non-saline soils was not affected. The amendments of ACC could not fully counteract the negative effects of salinity on soil microbial activities and productivity, but increased the abundance of ACC deaminase-encoding gene (acdS), enhanced soil microbial respiration, enzymatic activity, nitrogen and carbon cycling potentials and Arabidopsis biomass in saline soils. Collectively, our study indicates that ACC amendments in soils could efficiently ameliorate salinity impacts on soil properties and plant biomass production.

20.
Int J Cancer ; 2019 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-31081930

RESUMO

Akt is a promising therapeutic target for cancer treatment. In our study, we have identified that 7-deoxynarciclasine (7-DONCS) is a potential inhibitor of Akt, which results in the repression of multiple oncogenic processes in hepatocellular carcinoma (HCC). We have found that 7-DONCS suppresses the growth of HCC by inducing the apoptotic and autophagic capacities, as well as by inhibiting epithelial-mesenchymal transition (EMT) in vitro and in vivo. Pretreatment of cells with specific autophagy inhibitor (Bafilomycin A1) or knockdown of endogenous LC-3B by siRNA strongly abrogates 7-DONCS-regulated apoptosis and EMT. Consequently, we have found that 7-DONCS selectively inhibits phospho-Akt (Ser473), and subsequent molecular docking reveals that 7-DONCS directly binds to the C-terminal domain of Akt. Overexpressing Akt significantly blocks these effects via 7-DONCS in HCC cells. Furthermore, 7-DONCS, by targeting Akt, exhibits a promising therapeutic effect in orthotopic hepatocellular tumors. Finally, higher p-Akt expression is associated with poor prognosis, and higher level of Akt was positively correlated with the enrichment of both apoptosis and autophagy downregulation, and EMT upregulation in HCC patients. These studies suggest that 7-DONCS serves as an attractive drug candidate by targeting Akt for future HCC therapy.

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