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1.
Qual Life Res ; 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31898113

RESUMO

PURPOSE: Kidney transplant recipients suffer from a variety of symptoms, and symptom management is crucial in improving their quality of life (QOL). Accordingly, this study aims to identify the symptoms experienced by kidney transplant recipients and examine the relationship amongst antecedents, symptom experience and QOL of recipients in China. METHODS: A total of 300 kidney transplant recipients were investigated in one of the most first-rate hospitals in China by asking them to fill out a self-designed general questionnaire, the Symptom Experience Scale, the Sense of Coherence Scale (Chinese version), the Revised Life Orientation Test (Chinese version) and the 36-item Short Form Health Survey (Chinese version). RESULTS: Kidney transplant recipients experienced multiple symptoms that were frequent, severe and bothersome. The final model showed significant consistency with the data. In the QOL model, post-transplant time, complications, immunosuppressive agents and sense of coherence explained 30.7% of the variance of symptom experience. Moreover, habitual residence, economic burden, post-transplant time, kidney function, optimism and symptom experience accounted for 70.6% of the variance on QOL. CONCLUSIONS: We can explain the relationship between antecedents, symptom experience and QOL amongst Chinese kidney transplant recipients by using Symptoms Experience Model. Clinicians and caregivers can manage the recipient's symptoms during follow-up from psychological, physical and medication management perspectives. Improving sense of coherence, maintaining optimism and managing symptoms are essential for enhancing QOL.

2.
J Clin Gastroenterol ; 2020 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-31904680

RESUMO

BACKGROUND: Quantitative information is scarce with regard to guidelines for currently prescribed medications for constipation. Furthermore, these guidelines do not reflect the differences in the number of bowel movements caused by each drug. GOALS: In this study, we used a model-based meta-analysis to quantitatively estimate the deviations from the baseline number of spontaneous bowel movements (SBMs) and complete spontaneous bowel movements (CSBMs) associated with pharmacotherapy for chronic constipation to bridge the knowledge gap in the guidelines for current medications. STUDY: A comprehensive survey was conducted using literature databases. In this study, we also included randomized placebo-controlled trials on chronic constipation. Pharmacodynamic models were established to describe the time course of the numbers of SBMs and CSBMs produced by each drug. RESULTS: Data from 20 studies (comprising 9998 participants and 8 drugs) were used to build this model. The results showed that bisacodyl had the greatest effect on increasing the frequency of bowel movements, whereas plecanatide yielded the lowest increase in the number of SBMs and CSBMs. After eliminating the placebo effect, the maximal increase in bowel movement frequency associated with bisacodyl was 6.8 for SBMs (95% confidence interval: 6.1-7.6) and 4.7 for CSBMs (95% confidence interval: 4.3-5.1) per week. These numbers are ∼4 times higher than the number of bowel movements produced by plecanatide. The change in the frequency of SBMs and CSBMs for other drugs, such as sodium picosulfate, velusetrag, linaclotide, elobixibat, lubiprostone, and prucalopride, was similar. The highest increases in the frequency of SBM and CSBM were 2.5 to 4 and 1 to 2.1 per week, respectively. Bisacodyl had the most noticeable loss of efficacy between week 1 and week 4; it reduced the frequencies of SBMs and CSBMs by 2.3 and 2.2, respectively. By contrast, the changes in the frequencies of SBMs and CSBMs were not as great with other drugs. CONCLUSIONS: The data provided in this study may be a valuable supplement to the medication guidelines for the treatment of chronic constipation.

3.
Invert Neurosci ; 20(1): 1, 2020 01 21.
Artigo em Inglês | MEDLINE | ID: mdl-31960127

RESUMO

Proteins encoded by nanchung, inactive, nompC and piezo genes have been shown to play crucial roles in the initial detection of mechanical force by various insect auditory neurons, nociceptors and touch receptors. Most of this previous research has been performed on the larval and adult fruit fly, Drosophila melanogaster. We identified and assembled all four homologous genes in transcriptomes from the cockroach, Periplaneta americana. Injection of long double-stranded RNA (dsRNA) into the adult cockroach abdomen successfully reduced the expression of each gene, as measured by quantitative PCR (RT-qPCR). A simple electrophysiological assay was used to record action potential firing in afferent nerves of cockroach femoral tactile spines in response to a standardized mechanical step displacement. Responses of nanchung knockdown animals were significantly reduced compared to matched sham-injected animals at 14 and 21 days after injection, and inactive knockdowns similarly at 21 days. In contrast, responses of nompC and piezo knockdowns were unchanged. Our results support a model in which Nanchung and Inactive proteins combine to form a part of the mechanotransduction mechanism in the cockroach tactile spine.

4.
Ecotoxicol Environ Saf ; 191: 110145, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31954214

RESUMO

Arsenic (As) and antimony (Sb) are listed as the priority pollutants by the U.S. Environmental Protection Agency (EPA) and the European Union (EU) due to their toxicity and potential carcinogenicity. It is necessary to investigate their adsorption over soil as such a behavior affects their mobility and bioavailability. In this study, the effect of pH on the adsorption of As(V) and Sb(V) by the black soil was investigated with three systems: the Single system, Binary system, and Sequence system. The operating pH was set at 4.0, 7.0 and 10.0. Based on the Langmuir isothermal and the pseudo-second-order kinetic models, the adsorption for As(V) was always better than Sb(V) in the whole pH range; the best adsorption performance for the two sorbates was achieved at pH of 4.0, followed by 7.0 and 10.0 in the three systems. The reasons could be that the atomic radius of arsenic is smaller than that of antimony, and the positively charged functional groups carried by the inorganic colloids in the soil contributed to binding with the negatively charged As(V)/Sb(V). A lower pH promoted the inorganic colloids to carry more positive charges. Compared to Single system, the maximum adsorption capacity (qm) and the initial adsorption rates (k2qe,cal2) of As(V) and Sb(V) in Binary system decreased obviously, suggesting competitive adsorption occurred when As(V) and Sb(V) coexisted. The findings of this workimprove the understanding of As(V)/Sb(V) adsorption behavior in soil under different situations and would facilitate a comprehensive evaluation on the risk assessment of arsenic and antimony.

5.
J Cell Mol Med ; 24(2): 1541-1552, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31793207

RESUMO

Both PNPLA3 I148M and hepatic inflammation are associated with nonalcoholic fatty liver disease (NAFLD) progression. This study aimed to elucidate whether PNPLA3 I148M is involved in NF-kB-related inflammation regulation in NAFLD. HepG2 cells homozygous for the PNPLA3 I148M mutation were used. The human PNPLA3 promoter sequence was screened for NF-kB binding sites using the MATCH and PATCH tools. NF-kB-mediated transcriptional regulation of the PNPLA3 gene was assessed by luciferase reporter assay, EMSA and ChIP-qPCR. Wild-type (I148I) and mutant (M148M) PNPLA3 were overexpressed using stable lentivirus-mediated transfection. The pCMV vector and siRNA were transiently transfected into cells to direct NF-kB overexpression and PNPLA3 silencing, respectively. A putative NF-kB binding site in the human PNPLA3 promoter was shown to be necessary for basal and NF-kB-driven transcriptional activation of PNPLA3 and protein/DNA complex formation. Supershift analysis demonstrated a protein/DNA complex specifically containing the NF-kB p65 and p50 subunits. ChIP-qPCR confirmed the endogenous binding of NF-kB to the human PNPLA3 promoter in response to NF-kB overexpression and palmitic acid (PA) challenge. The silencing of PNPLA3 blocked the overexpression of NF-kB or PA-induced TNF-α up-regulation. Moreover, mutant PNPLA3 overexpression prevented NF-kB inhibitor-induced down-regulation of TNF-α expression in PA-treated HepG2 cells. Finally, the overexpression of mutant but not wild-type PNPLA3 increased TNF-α expression and activated the ER stress-mediated and NF-kB-independent inflammatory IRE-1α/JNK/c-Jun pathway. Human PNPLA3 was shown to be a target of NF-kB, and PNPLA3 I148M mediated the regulatory effect of NF-kB on inflammation in PA-treated HepG2 cells, most likely via the IRE-1α/JNK/c-Jun ER stress pathway.

6.
Bull Environ Contam Toxicol ; 104(1): 96-106, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31729560

RESUMO

During the summer of 2015, polycyclic aromatic hydrocarbons (PAHs) in the atmosphere were collected by passive air samplers in typical urban-rural fringe of Wuhan-Ezhou region, Central China. The results showed that 16 kinds of PAHs were ubiquitous with the concentrations of ∑16PAHs from 14.69 to 136.30 ng·m-3 and the mean concentration of 43.03 ng·m-3. Phenanthrene (Phe), fluoranthene (Fla) and pyrene (Pyr) were major components, which accounted for 81% of ∑16PAHs. PAHs atmospheric concentrations presented obvious spatial variation, being significantly related to geographical environment and influenced by anthropogenic activity. Air-soil exchange status of PAHs was discussed according to the fugacity fraction (ff). The results showed that HMW-PAHs behaved as net deposition, while LMW-PAHs were more likely to establish dynamic equilibrium between atmosphere and soil than MMW-PAHs and HMW-PAHs. For some PAHs, such as acenaphthylene (Acy) and anthracene (Ant), the soil acted as second sources of them.

7.
Phytopathology ; 110(2): 309-316, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31556343

RESUMO

Rice yield is greatly reduced owing to rice blast, a polycyclic fungal disease caused by the ascomycete Magnaporthe oryzae. Previously, Bacillus cereus HS24, isolated from a rice farm, showed a strong antimicrobial effect toward M. oryzae. To better exploit it as a biocontrol agent, HS24 was studied for the mechanism that it uses to suppress rice blast. Conidium germination in M. oryzae was significantly inhibited by HS24, whereby inhibition reached 97.8% at the concentration of 107 CFU/ml. The transcription levels of Ca2+/calmodulin-dependent protein kinase II, PMC1, and CCH1, key genes involved in the M. oryzae Ca2+ signaling pathway, were significantly decreased in HS24-treated conidia at high concentration. The treatment of M. oryzae with the corresponding Ca2+ signaling pathway inhibitors KN-93, verapamil, and cyclopiazonic acid significantly reduced conidium germination. This inhibitory effect was found to be concentration dependent, similar to the HS24 treatment. We also found that HS24 was able to decrease the intracellular free Ca2+ concentration in M. oryzae conidia significantly. The addition of exogenous Ca2+ did not diminish the inhibitory effect of HS24 on the reduction of intracellular free Ca2+ concentration and the level of conidium germination. In conclusion, B. cereus HS24 at high concentration prevents extracellular Ca2+ from entering the conidia in M. oryzae, causes a significant reduction of intracellular free Ca2+ concentration, and results in the inhibition of conidium germination.

8.
Int J Mol Med ; 45(1): 186-194, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31746354

RESUMO

Senescence of cardiomyocytes is considered a key factor for the occurrence of doxorubicin (Dox)­associated cardiomyopathy. The NOD­like receptor family pyrin domain­containing 3 (NLRP3) inflammasome is reported to be involved in the process of cellular senescence. Furthermore, thioredoxin­interactive protein (TXNIP) is required for NLRP3 inflammasome activation and is considered to be a key component in the regulation of the pathogenesis of senescence. Studies have demonstrated that pretreatment with honokiol (Hnk) can alleviate Dox­induced cardiotoxicity. However, the impact of Hnk on cardiomyocyte senescence elicited by Dox and the underlying mechanisms remain unclear. The present study demonstrated that Hnk was able to prevent Dox­induced senescence of H9c2 cardiomyocytes, indicated by decreased senescence­associated ß­galactosidase (SA­ß­gal) staining, as well as decreased expression of p16INK4A and p21. Hnk also inhibited TXNIP expression and NLRP3 inflammasome activation in Dox­stimulated H9c2 cardiomyocytes. When TXNIP expression was enforced by adenovirus­mediated gene overexpression, the NLRP3 inflammasome was activated, which led to inhibition of the anti­inflammation and anti­senescence effects of Hnk on H9c2 cardiomyocytes under Dox treatment. Furthermore, adenovirus­mediated TXNIP­silencing inhibited the NLRP3 inflammasome. Consistently, TXNIP knockdown enhanced the anti­inflammation and anti­senescence effects of Hnk on H9c2 cardiomyocytes under Dox stimulation. In summary, Hnk was found to be effective in protecting cardiomyocytes against Dox­stimulated senescence. This protective effect was mediated via the inhibition of TXNIP expression and the subsequent suppression of the NLRP3 inflammasome. These results demonstrated that Hnk may be of value as a cardioprotective drug by inhibiting cardiomyocyte senescence.

9.
Water Res ; 168: 115164, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31629229

RESUMO

Carbamazepine (CBZ) is a typical pharmaceutical residue commonly found in aqueous environments, but its removal through activated carbon or advanced oxidation processes is often disrupted by co-existing organic matter. An imprinting system which consisted of the target pollutant CBZ (template molecule) and 10 different kinds of functional monomers was constructed via molecular simulation to screen for appropriate monomers, thereby addressing CBZ removal disruptions. An annealing method simulation was used to search for stable, low-energy conformations of the template-monomer interaction system to calculate the binding energy of these different monomers with CBZ. The order of binding affinity calculated was: 4-vinylbenzoic acid > itaconic acid > methacrylic acid, which was consistent with the experimental observations. The adsorption capacity of the molecular imprinted polymer (MIP) prepared using 4-vinylbenzoic acid reached 28.40 mg/g, and the imprinting factor reached 2.72. The simulation and measurement of the ultraviolet spectrum of the imprinting system showed that a new interaction system was formed between the template and monomers, and that multiple binding conformations between them took place when specific recognition occurred. Energy calculation and hydrogen bond analysis revealed that the van der Waals force, including the π-π conjugate and electrostatic forces including hydrogen bonding, played an important role during selective adsorption, which was confirmed by infrared spectroscopy analysis.


Assuntos
Poluentes Ambientais , Impressão Molecular , Preparações Farmacêuticas , Adsorção , Carbamazepina
10.
J Food Sci ; 85(1): 123-131, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31872874

RESUMO

Adlay oil (AO) is an important component of adlay seeds that has many beneficial functions to human health. In this study, the variations in the chemical composition and antioxidant activity of AOs extracted from adlay samples obtained from four main producing areas of China were first investigated. Results revealed that the AO of the adlay samples varied from 7.398 ± 0.486% to 8.464 ± 0.725%. The highest contents of total fatty acid, triolein, total phenolic, and total flavonoid were observed in Xingren AO, whereas coixol content was found to be highest in Pucheng AO. The AO samples were grouped successfully in accordance with the origins by partial least squares-discriminant analysis and hierarchical cluster analysis based on the contents of the chemical components. Furthermore, AOs exhibited considerable levels of 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity (IC50 , 0.924 ± 0.113 to 1.116 ± 0.109 mg/mL) radical scavenging activity, ferric-reducing antioxidant power (FRAP) activity (EC50 , 0.019 ± 0.002 to 0.028 ± 0.002 mg/mL) activity, and ß-carotene-linoleic acid bleaching activity (IC50, 0.233 ± 0.008 to 0.414 ± 0.012 mg/mL) activity. Total phenolic and total flavonoid both demonstrated highest correlation with ABTS values (r = -0.952 and r = -0.960, respectively). The results derived from above studies suggest that geographic origin has a certain influence on the phytochemical profiles and antioxidant activity of the AOs. PRACTICAL APPLICATION: The chemical composition and antioxidant activity of adlay oils extracted from the adlay samples obtained from four main producing areas of China were evaluated, including total fatty acid, triolein, total phenolic, and total flavonoid, and ABTS radical scavenging activity, FRAP activity, and ß-carotene-linoleic acid bleaching activity.

11.
Sleep ; 2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31781753

RESUMO

STUDY OBJECTIVES: This study aimed to develop a robust placebo response model for the pharmacotherapy for insomnia to guide drug development and clinical practice. METHODS: PubMed, EMBASE, and Cochrane Library databases were systematically searched for randomized placebo-controlled trials of medications for insomnia dating from the inception dates of the databases to April 18, 2018. Three placebo response models were established to describe the time-course of sleep parameters measured by objective (polysomnography or actigraphy) or subjective methods (sleep diary or questionnaires). The established models were applied to simulate placebo response distribution under different conditions using Monte Carlo simulations. RESULTS: Fifty-four studies involving 6,416 subjects were included. Placebo response increased over time and reached a plateau at approximately 8 weeks from start of therapy. Established models described the observed data reasonably well based on various diagnostic plots. Baseline sleep parameters affected the placebo response. There were significant positive correlations with placebo response and the severity of sleep latency, wake after sleep onset, and total sleep time at baseline. In addition, placebo response, assessed by subjective and objective methods, was consistent after correcting the baseline levels. CONCLUSIONS: The established placebo response models can serve as a tool to predict placebo response at different baseline levels, which can provide valuable reference for clinical trial design, decision-making in drug development, and clinical practice.

12.
Environ Sci Pollut Res Int ; 26(35): 35657-35669, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31732950

RESUMO

Mine tailings, generated from the extraction, processing, and utilization of mineral resources, have resulted in serious acid mine drainage (AMD) pollution. Recently, scholars are paying more attention to two alternative strategies for resource recovery and ecological reclamation of mine tailings that help to improve the current tailing management, and meanwhile reduce the negative environmental outcomes. This review suggests that the principles of geochemical evolution may provide new perspective for the future in-depth studies regarding the pollution control and risk management. Recent advances in three recycling approaches of tailing resources, termed metal recovery, agricultural fertilizer, and building materials, are further described. These recycling strategies are significantly conducive to decrease the mine tailing stocks for problematic disposal. In this regard, the future recycling approaches should be industrially applicable and technically feasible to achieve the sustainable mining operation. Finally, the current state of tailing phytoremediation technologies is also discussed, while identification and selection of the ideal plants, which is perceived to be the excellent candidates of tailing reclamation, should be the focus of future studies. Based on the findings and perspectives of this review, the present study can act as an important reference for the academic participants involved in this promising field.

13.
Aging (Albany NY) ; 11(21): 9643-9660, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31689236

RESUMO

In this study, we investigated the role of ubiquitin-specific protease 22 (USP22) in the growth and progression of gastric cancer (GC). USP22 mRNA and protein levels were significantly higher in GC tissue samples and GC cell lines than in adjacent noncancerous tissue samples and a normal gastric mucosal epithelial cell line (GES1), respectively. USP22 knockdown significantly decreased in vitro survival, proliferation, migration, and invasiveness of GC cells compared with the controls. Western blot analysis of control and USP22-silenced GC cells showed that USP22 modulates the c-Myc/NAMPT/SIRT1-dependent FOXO1 and YAP signaling pathways. Subcutanenous injection of USP22-silenced GC cells into SCID mice generated significantly smaller xenograft tumors than did control cells. Moreover, USP22-silenced GC cells showed less lung metastasis than the controls following tail vein injection in SCID mice. In addition, high USP22 expression correlated positively with tumor size, advanced stage and metastasis, and correlated negatively with tumor differentiation and prognosis in GC patients. These results show that USP22 regulates growth and progression of GC via the c-Myc/NAMPT/SIRT1-dependent FOXO1 and YAP signaling pathways.

14.
Int J Mol Med ; 44(6): 2065-2076, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31638187

RESUMO

Post­infarction remodeling is accompanied and influenced by perturbations in the mammalian target of rapamycin (mTOR) signaling. Regulated in development and DNA damage response­1 (Redd1) has been reported to be involved in DNA repair and modulation of mTOR activity. However, little is known about the role of Redd1 in the heart. In the present study the potential contribution of Redd1 overexpression to the chronic phase of heart failure after myocardial infarction (MI) was explored and the mechanisms underlying Redd1 actions were determined. Redd1 was downregulated in the mouse heart subjected to MI surgery. To determine the role of Redd1 in the process of MI, adeno­associated virus 9 mediated overexpression of Redd1 was used to enhance Redd1 content in cardiomyocytes. Redd1 overexpression improved left ventricular dysfunction and reduced the expansion index. Additionally, Redd1 overexpression resulted in suppressed myocardial apoptosis and improved autophagy. Furthermore, the studies revealed that Redd1 overexpression could inhibit the phosphorylation of mTOR and its downstream effectors P70/S6 kinase and 4EBP1. In conclusion, this study demonstrated that Redd1 overexpression protects against the development and persistence of heart failure post MI by reducing apoptosis and enhancing autophagy via the mTOR signaling pathway. The present study clearly demonstrated that Redd1 is a therapeutic target in the development of heart failure after MI.

15.
Mol Med Rep ; 20(6): 4855-4866, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31638204

RESUMO

Aberrant expression of the epidermal growth factor receptor (EGFR) plays vital roles in tumor development and progression. In the present study, ultraviolet irradiation induced the upregulation of EGFR in skin­derived keratinocytes, which may contribute to the development of cutaneous squamous cell carcinoma (CSCC). This was supported by the high expression of EGFR in CSCC clinical samples. Treating A431 CSCC cells with gefitinib, a tyrosine kinase inhibitor, activated the intrinsic mitochondrial apoptotic pathway while inducing protective autophagy. Combined application of chloroquine with gefitinib enhanced the treatment efficacy of gefitinib against CSCC by inhibiting autophagic flux. These findings demonstrated that autophagy inhibition may be an effective strategy for enhancing the sensitivity of EGFR­expressing cells to tyrosine kinase inhibitor treatment. Manipulating pro­survival autophagy by the combined application of chloroquine with gefitinib is a promising approach for improving the efficacy of EGFR inhibitors in cancer treatment. This may contribute to novel EGFR­targeted therapeutic strategies in the near future.

16.
Aging (Albany NY) ; 11(17): 7021-7035, 2019 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-31494646

RESUMO

Aging is associated with an increased risk of cardiovascular disease. Numerical and functional declines in endothelial progenitor cells (EPCs) limit their capacity for endothelial repair and promote the development of cardiovascular disease. We explored the effects of nuclear factor (erythroid-derived 2)-like 2 (NRF2) on EPC activity during aging. Both in vitro and in vivo, the biological functioning of EPCs decreased with aging. The expression of NRF2 and its target genes (Ho-1, Nqo-1 and Trx) also declined with aging, while Nod-like receptor protein 3 (NLRP3) expression increased. Aging was associated with oxidative stress, as evidenced by increased reactive oxygen species and malondialdehyde levels and reduced superoxide dismutase activity. Nrf2 silencing impaired the functioning of EPCs and induced oxidative stress in EPCs from young mice. On the other hand, NRF2 activation in EPCs from aged mice protected these cells against oxidative stress, ameliorated their biological dysfunction and downregulated the NLRP3 inflammasome. These findings suggest NRF2 can prevent the functional damage of EPCs and downregulate the NLRP3 inflammasome through NF-κB signaling.

17.
Nanomaterials (Basel) ; 9(9)2019 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-31480685

RESUMO

Transition metal dichalcogenides (TMDCs) have received wide attention as a new generation of semiconductor materials. However, there are still many problems to be solved, such as low carrier mobility, contact characteristics between metal and two-dimensional materials, and complicated fabrication processes. In order to overcome these problems, a large amount of research has been carried out so that the performance of the device has been greatly improved. However, most of these studies are based on complicated fabrication processes which are not conducive to the improvement of integration. In view of this problem, a horizontal-gate monolayer MoS2 transistor based on image force barrier reduction is proposed, in which the gate is in the same plane as the source and drain and comparable to back-gated transistors on-off ratios up to 1 × 104 have been obtained. Subsequently, by combining the Y-Function method (YFM) and the proposed diode equivalent model, it is verified that Schottky barrier height reduction is the main reason giving rise to the observed source-drain current variations. The proposed structure of the device not only provides a new idea for the high integration of two-dimensional devices, but also provides some help for the study of contact characteristics between two-dimensional materials and metals.

18.
Data Brief ; 25: 104345, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31485468

RESUMO

This data article provides supporting information to a related research article "Identification of volatile organic compounds for the biocontrol of postharvest litchi fruit pathogen Peronophythora litchii" (Zheng et al., 2019) [1]. The litchi downy blight (LDB) caused by Peronophythora litchii is a major postharvest disease that can severely damage litchi trees and harvested litchi fruit. This data article describes the analysis of volatile compounds (VOCs) in three bacterial biological control agents (BCAs) of LDB (Bacillus amyloliquefaciens PP19, Bacillus pumilus PI26, and Exiguobacterium acetylicum SI17) via gas chromatography/mass spectrometry (GC-MS). Volatile compounds produced by the three BCAs were captured at five culture time of 24, 36, 48, 60 and 72 h by a solid-phase micro extraction method. The chemical compositions were identified and their retention times as well as relative peak areas were analyzed. Compounds commonly produced at more than one time points were then subjected to in vitro (on petri dish) and in vivo (litchi fruit and leaves) evaluations for their antagonistic activities against the pathogen Peronophythora litchii.

19.
Carbohydr Polym ; 224: 115202, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31472833

RESUMO

We report here the development of transparent and strong polymer composites reinforced by unmodified cellulose nanofibrils (CNFs) with a Pickering emulsion gelation strategy. The CNFs entangle and firmly stabilize on the surface of emulsion droplets containing polymethyl methacrylate (PMMA) solution, leading to the gelation of the emulsions. CNFs/PMMA composites were generated via vacuum filtration and solvent washing of the gel and a subsequent two-step hot pressing. The composites contained a unique self-assembled two-tier hierarchy of CNFs networks and demonstrate promising transparency, tensile strength, flexibility, and an extremely low thermal expansion. Remarkably, these properties are highly tunable with varying the concentration of CNFs and the volume ratio of the water to oil phase. This work offers a facile route to realize the well dispersion of unmodified CNFs in hydrophobic polymer matrix and achieve high performance of polymeric materials reinforced by CNFs.

20.
Eur J Clin Pharmacol ; 75(11): 1533-1540, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31401678

RESUMO

PURPOSE: The purpose of this study was to investigate the potential impact of CYP3A4, CYP3A5, and CYP3A7 polymorphisms on the concentration and efficacy of tacrolimus in a cohort of pediatric patients with nephrotic range proteinuria. METHODS: Genetic variants including CYP3A5*3 (rs776746), CYP3A4*1G (rs2242480), rs4646437, and CYP3A7 rs2257401 and rs10211 were detected in 70 pediatric patients with nephrotic range proteinuria. The relationships of dose-adjusted trough concentration (C0) of tacrolimus with corresponding genotypes were investigated. RESULTS: The tacrolimus concentration in patients without CYP3A5*3 A allele was 94% higher than those with A allele (90.7 vs 54.2, P = 0.00006). The CYP3A7 rs2257401 was also associated with the concentration of tacrolimus. The C allele carriers had an obviously lower C0 than the non-carriers (62.4 vs 90.7, P = 0.001). In addition, there were significant differences in tacrolimus concentration among CYP3A7 rs10211 G carriers and non-carriers; the latter had an almost twofold C0 of the former (101.8 vs 59.6, P = 0.0004). CONCLUSIONS: Our study demonstrated the associations between CYP3A5*3, CYP3A7 rs2257401 and rs10211, and tacrolimus concentration in pediatric patients with nephrotic range proteinuria. Children with CYP3A5*3 A, CYP3A7 rs2257401 C, and rs10211 G alleles might need a higher dose of tacrolimus.

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