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1.
Medicine (Baltimore) ; 99(3): e18869, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32011510

RESUMO

RATIONALE: The frequency of tooth avulsion is on the rise due to increasing rates of maxillofacial trauma. Avulsed teeth present with varying degrees of root resorption, and are generally asymptomatic; therefore, they often go undiagnosed. The etiopathogenesis of root resorption in replanted teeth following avulsion remains unclear. PATIENT CONCERNS: In case 1, the left upper lateral incisor became loose after 10 years of replantation. In case 2, the patient underwent tooth replantation after external root canal treatment due to tooth dislocation caused by trauma 8 years ago. DIAGNOSIS: According to the medical history, clinical manifestations and imaging studies of the 2 patients, root resorption after replantation was diagnosed. INTERVENTIONS: The teeth extraction was given to one patient. Besides the histological examination of extracted teeth was performed. OUTCOMES: Teeth that underwent pulp treatment presented with external resorption. On the other hand, the tooth that had received no pulp treatment showed both external and internal resorption; residual vital pulp tissue was detected within the pulp cavity. LESSONS: The dental pulp tissues may be involved in the initiation or development of internal resorption. Trauma to the periodontal ligament might play a major role in external resorption, whereas internal tooth resorption may be caused as a result of injury to the residual pulp tissue. Thus, the effective management of these tissues during the treatment of replanted teeth is essential.

2.
Aging (Albany NY) ; 122020 Feb 16.
Artigo em Inglês | MEDLINE | ID: mdl-32062614

RESUMO

Previous Mendelian randomization (MR) studies have yielded a conflicting causal relationship between sarcopenia and coronary artery disease (CAD), and lack the association of CAD with sarcopenia. We performed a bi-directional MR approach to clarify the causality and causal direction between sarcopenia-related traits and CAD. In stage 1 analysis, estimates of inverse variance weighting (IVW) and several sensitivity analyses were obtained by applying genetic variants that predict sarcopenia-related traits to CAD. Conversely, we also applied genetic variants that predict CAD to sarcopenia-related traits in stage 2 analyses. IVW analysis showed that higher handgrip strength reduces risk for CAD: A 1-kilogram (kg) increase in genetically determined left handgrip strength reduced odds of CAD by 36% [odds ratio (OR) = 0.64, 95% confidence interval (CI) 0.498 - 0.821, p = 4.56E-04], and right handgrip strength reduced odds of CAD by 41.1% (OR = 0.599, 95% CI 0.476 - 0.753, p = 1.10E-05). However, genetically predicted CAD did not show any causal association with handgrip strength, and no significant causal relationship was detected between genetically instrumented body lean mass and CAD. Our results suggest that decreased muscle strength but not decreased muscle mass leads to the increased risk of CAD in sarcopenia.

3.
Planta ; 251(2): 47, 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31925576

RESUMO

MAIN CONCLUSION: Circular RNA (circRNA) identification and expression profiles, and construction of circRNAs-miRNAs-mRNAs networks indicates that circRNAs are involved in wood formation of poplars in acclimation to low nitrogen availability. Circular RNAs (circRNAs) are covalently closed non-coding RNAs that play pivotal roles in various biological processes. However, circRNAs' roles in wood formation of poplars in acclimation to low nitrogen (N) availability are currently unknown. Here, we undertook a systematic identification and characterization of circRNAs in the wood of Populus × canescens exposed to either 50 (low N) or 500 (normal N) µM NH4NO3 using rRNA-depleted RNA-sequencing. A total of 2,509 unique circRNAs were identified, and 163 (ca. 6.5%) circRNAs were significantly differentially expressed (DE) under low N condition. We observed a positive correlation between the expression patterns of DE circRNAs and their hosting protein-coding genes. Moreover, circRNAs-miRNAs-mRNAs' networks were identified in the wood of poplars under low N availability. For instance, upregulated several circRNAs, such as circRNA1226, circRNA 1732, and circRNA392 induced increases in nuclear factor Y, subunit A1-A (NFYA1-A), NFYA1-B, and NFYA10 transcript levels via the mediation of miR169b members, which is in line with reduced xylem width and cell layers of the xylem in the wood of low N-supplied poplars. Upregulation of circRNA1006, circRNA1344, circRNA1941, circRNA901, and circRNA146 caused increased transcript level of MYB61 via the mediation of a miR5021 member, corresponding well to the higher lignin concentration in the wood of low N-treated poplars. Overall, these results indicated that DE circRNAs play an essential role in regulating gene expression via circRNAs-miRNAs-mRNAs' networks to modulate wood anatomical and chemical properties of poplars in acclimation to low N availability.

4.
Inorg Chem ; 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31990186

RESUMO

Tumor hypoxia is known to be one of the vital factors that aggravate tumor resistance to radiation therapy (RT) in which oxygen plays a critical role in tumor destruction. Herein, we synthesize a simple nanoradiosensitizer based on ultrathin BiO2-x nanosheets (NSs) modified with Tween 20 (T-BiO2-x NSs) to overcome the hypoxia-induced radioresistance as well as increase the efficacy of RT. On the one hand, bismuth as a high-Z element can effectively enhance the sensitivity of RT by depositing a higher radiation dose in tumors. The semiconductor property also endows its photocatalytic ability to produce extra reactive oxygen species (ROS) by reaction with the surrounding water. On the other hand, the defect-abundant BiO2-x NSs are also found to decompose the highly expressed hydrogen peroxide (H2O2) at the tumor site into oxygen (O2) for combating hypoxia. Both in vitro and in vivo experiments indicate that the as-prepared T-BiO2-x NSs could effectively inhibit tumor growth with X-ray irradiation. Our work thus provides a simple nanoradiosensitizer with multifunctionalities for increasing the RT efficacy while alleviating tumor hypoxia at the same time.

5.
Phys Chem Chem Phys ; 22(4): 2521-2529, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31939952

RESUMO

The guest anions play a key role in the construction of layered double hydroxide (LDH)-based host-guest functional materials. In this work, the orientation of the interlayer species, interlayer distances, binding energies, electronic density differences and density of states of the MgnAl-LDHs (n = 1.6, 2.0, 2.6, 3.5, 5.0, and 8.0) with nine different anions (F-, Cl-, Br-, I-, OH-, NO3-, CO32-, SO42-, and PO43-) are calculated by density functional theory (DFT). The results reveal that the LDHs containing the anions with more inclined arrangement, larger size, lower charge and with a larger number of interlayer water molecules show larger interlayer distances. The higher anion charge leads to a larger binding energy for LDHs, and the order of binding energy implies that the sequence of anion exchange is PO43- > CO32- > SO42- > OH- > F- > Cl- > Br- > NO3- > I-. The interactions between interlayer species and the host layer or the interlayer water molecules are mainly derived from the electrostatic interactions. The main components of the valence band maximum (VBM) and conduction band minimum (CBM) of MgAl-LDHs are derived from p orbitals of halogen anions or the O-2p orbitals of other anions, and the Mg-2p orbital, respectively. This illustrates that the most basic sites of MgAl-LDHs are the interlayer anions rather than the hydroxyl group in the layer, while the most acidic sites are Mg in the layers. And LDHs containing anions with higher charge show stronger basicity. The calculation results agree well with the experimental findings. This work provides effective theoretical information for the design and preparation of the anion-controlled functional LDHs or related materials with prospective applications.

6.
Pharmacology ; : 1-11, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31910411

RESUMO

BACKGROUND: Gastric cancer has become the second major cause of cancer death. The aim of the present study was to explore the relationship between miR-21 and the long noncoding RNA growth arrest-specific 5 (GAS5) in gastric cancer and the effect on gastric cancer cells. METHODS: The expression of miR-21 and GAS5 mRNA was analyzed by quantitative real-time-PCR. Overexpression of GAS5 was used to investigate the biological functions of GAS5 in cells. The cell proliferation was detected by cell counting kit-8 assay and the cell migration and invasion were detected by Transwell. Cell apoptosis was evaluated by Annexin V-FITC/PI staining and apoptosis-related proteins were detected by western blot. The mechanism of GAS5 in vivo was evaluated by the tumorigenesis of nude mice, and dual luciferase reporter was used to determine if miR-21 is a GAS5 target. The inhibition of miR-21 and the simultaneous overexpression of GAS5 and miR-21 were further performed, and the above indicators were detected again. RESULTS: GAS5 was low expression and miR-21 was high expression in gastric cancer tissues and cells. GAS5 overexpression reduced the proliferation, migration, and invasion of gastric cancer cells and increased the apoptosis of gastric cancer cells. The growth rate of GAS5 group slowed down and the volume of tumor decreased. miR-21 is a GAS5 target and GAS5 inhibits the proliferation of gastric cancer cells by targeting miR-21. CONCLUSION: Our research shown that overexpression of GAS5 can significantly inhibit the proliferation, migration, invasion and tumor formation of gastric cancer cells, and promote the apoptosis of gastric cancer cells, which may be related to the targeting inhibition of miR-21 expression by GAS5.

7.
Sci Total Environ ; 705: 135905, 2020 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-31838423

RESUMO

In this work, a novel dual signal amplification strategy for aptasensor employing reduced graphene with silver nanoparticles and prussian blue-gold nanocomposites was developed for detection of acetamiprid. To improve the sensitivity of aptasensors, reduced graphene oxide-silver nanoparticles (rGo-AgNPs) were modified on a bare glassy carbon electrode surface, which provided a large specific surface area for subsequent material immobilization and amplified current signal. The electrical signal output and sensitivity of the aptasensor was significantly improved after the immobilization of prussian blue-gold nanoparticles (PB-AuNPs) as a catalyst for the redox reaction. The analysis experiment exhibited that it had super-high sensitivity with a detection limit of 0.30 pM (S/N = 3), which met the requirements of the vast majority of daily leaf vegetable testing. Under optimized conditions, the proposed aptasensor showed a wide linear detection range from 1 pM to 1 µM. This aptasensor also had good stability and high selectivity for acetamiprid detection without an interfering effect of some other pesticides. The proposed aptasensor displayed good recovery rates in real samples, which proposed a new method for constructing electrochemical sensors and provided a novel tool for rapid, sensitive analysis of pesticides with low cost.

8.
Plant Physiol ; 182(1): 626-639, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31694900

RESUMO

Temperature has a large impact on plant immune responses. Earlier studies identified intracellular immune receptor nucleotide-binding leucine-rich repeat (NLR) genes and salicylic acid (SA) as targets of high-temperature inhibition of plant immunity. Here, we report that moderately low temperature enhances immunity to the bacterial pathogen Pseudomonas syringae in Arabidopsis (Arabidopsis thaliana). This enhancement is dependent on SA signaling and is accompanied by up-regulation of multiple SA biosynthesis and signaling genes at lower temperature. SA signaling is repressed by jasmonic acid and ethylene at both normal and low temperatures. The inhibition of SA biosynthesis by ethylene, while mainly through ISOCHORISMATE SYNTHASE1/SALICYLIC ACID-INDUCTION DEFICIENT2 (ICS1/SID2) at normal temperature, is through ENHANCED DISEASE SUSCEPTIBILITY5 (EDS5)/SID1, ICS2, and ICS1/SID2 at lower temperature. The repression by ethylene is mediated by a direct regulation of the ethylene response transcription factor ETHYLENE INSENSITIVE3 (EIN3) on multiple SA biosynthesis and signaling genes. Thus, low temperature enhances the SA pathway to promote immunity and at the same time uses ethylene to repress multiple SA regulators to achieve fine-tuned immune responses.

9.
Bioorg Med Chem Lett ; 30(2): 126848, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31836443

RESUMO

A series of pyrrolo[2,3-b]pyridine derivatives bearing 4-oxoquinoline moiety were designed, synthesized and evaluated for the anti-proliferative on three cancer cell lines (A549, HepG2 and MCF-7) in vitro. Most of the compounds showed moderate to high potency. Some excellent compounds were tested for the inhibitory activity of c-Met kinase. Compound 34 (c-Met IC50 = 17 nM) was investigated the selectivity against Flt-3, c-Kit, VEGFR-2, ALK, PDGFR-ß and RON. Structure-activity relationship studies indicated that hydrogen, fluorine atom, and mono-electron-withdrawing groups (mono-EWGs, such as R2 = F) on R, R1 and R2, respectively, were beneficial for the anti-proliferative activities of the target compounds. Besides, we have took further study on the combined mode between compound 34 and c-Met kinase through molecular docking.

10.
Environ Pollut ; 257: 113639, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31796315

RESUMO

Epidemiological studies have investigated the associations of bisphenol A (BPA) exposure with hypertension risk or blood pressure levels, but findings are inconsistent. Furthermore, the association between its alternatives bisphenol S and F (BPS and BPF) and hypertension risk are not yet known. We conducted a cross-sectional study in 1437 eligible participants without hypertension-related diseases, with complete data about blood pressure levels, hypertension diagnosis, and urinary bisphenols concentrations. Multivariable logistic and linear models were respectively applied to examine the associations of urinary bisphenols concentrations with hypertension risk and blood pressure levels. The dose-response relationship was explored by the restricted cubic spline model. Compared with the reference group of BPA, individuals in the middle and high exposure group had an adjusted odds ratio (OR) of 1.30 and 1.40 for hypertension, had a 3.08 and 2.82 mm Hg higher systolic blood pressure (SBP) levels, respectively, with an inverted "U" shaped dose-response relationship. Compared with the reference group of BPS, individuals in the second and third tertile had an adjusted OR of 1.49 and 1.48 for hypertension, had a 2.61 and 3.89 mm Hg increased levels of SBP, respectively, with a monotonic curve. No significant associations of BPF exposure with hypertension risk or blood pressure levels were found. BPA and BPS exposure were suggested to be associated with increased hypertension risk and blood pressure levels, with different dose-response relationships. Our findings have important implications for public health but require confirmation in prospective studies.

11.
ACS Appl Mater Interfaces ; 12(2): 2687-2694, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31860262

RESUMO

Silicon germanium (SiGe) alloys hold promise for thermoelectric power generation at high temperatures and have been applied in deep-space missions. However, enhancement of the dimensionless thermoelectric figure-of-merit (ZT) is still needed for practical civil applications of SiGe. In this work, we report high-performance oxide/SiGe bulk composites that were obtained via hot-press sintering of mixed powders composed of phosphorus (P)-doped SiGe prepared via mechanical alloying, using a ball-milling technique and La-Nb-doped SrTiO3 (La-Nb-STO). The La-Nb-STO powder was obtained from ball milling of a bulk La-Nb-STO sample that was sintered via hot pressing of hydrothermally synthesized La-Nb-STO powder. Controlling the amount of La-Nb-STO nanoparticles added to SiGe matrix increased the power factor by optimizing the electron concentration and mobility in the composite. In addition, compared with single-phase P-doped SiGe, the second phase decreased the thermal conductivity because of additional phonon scattering at the interface. As a result, a high ZT of 0.91 was realized in the n-type oxide/SiGe bulk composite at 1000 K, which was 18% larger than that for the typical materials used in space flight missions and 5% higher than the single-phase SiGe alloys obtained in the present study. The strategy used in this study could also be viable to further enhance the ZT of nanostructured n-type SiGe and SrTiO3-based oxide materials.

12.
J Drug Target ; : 1-16, 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31868032

RESUMO

Because the induction of strong host antitumor responses plays a very important role in antitumor therapy, identifying effective approaches to elicit immunogenic cell death could have important implications. RIP3-dependent necroptotic cancer cells have been reported to release damage-associated molecular patterns and enhance antitumor immunity. In this study, hyaluronic acid-conjugated cationic liposomes (DOTAP/DOPE/PEG-DSPE/CHOL) (HA-P-LP) were prepared as a vector for mRIP3-pDNA overexpression in tumours. Compared with standard cationic liposomes, this vector markedly increased cellular gene internalisation in vitro, enhanced the tumour-targeting effect in vivo and exhibited a significant antitumor effect in combination with adjuvant chloroquine. Considering the dramatic increase in RIP3 under the pathological condition of pancreatitis and the correlation between pancreatitis and necroptosis, non-HA-conjugated liposomes with the same formulation loaded with shRNA mRIP3-pDNA effectively controlled the disease by decreasing the serum amylase concentration and inflammatory cell infiltration. The versatile cationic liposomes loaded with plasmids with opposing functions in this study provide a new concept and method for both tumour therapy and pancreatitis therapy.

13.
R Soc Open Sci ; 6(11): 181795, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31827814

RESUMO

In the present study, uniaxial compression tests were conducted on sandstone-coal composite samples to investigate the effects of original macro-cracks in coal on the rockburst tendency. First, the energy dissipation theory was used to derive the elastic energy attenuation index of composite samples during uniaxial loading. Then, based on the test results obtained, the rockburst tendency of composite samples was evaluated and analysed using the uniaxial compressive strength and elastic energy attenuation index. The results show that the original macro-cracks in coal deteriorated the rockburst tendency of composite samples. The original horizontal cracks had the lowest effect on the rockburst tendency, whereas the vertical penetrating cracks through the coal centre (parallel to the loading direction) displayed the greatest effect. The mechanism by which these macro-cracks weakened the rockburst tendency involved two steps: (i) changing the physical properties and energy accumulation conditions of composite samples and (ii) increasing the energy dissipation of composite samples during uniaxial loading. These aspects are important to understand the rockburst hazards induced by the structural instability and failure of the composite system of coal seam and roof rock during deep coal mining.

14.
Transl Oncol ; 13(2): 245-253, 2019 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-31869749

RESUMO

Breast cancer is the most commonly diagnosed cancer and the leading cause of cancer death among women. Various mechanisms are involved in the initiation and progression of breast cancer. Metabolic dysregulation has been associated with increasing breast cancer incidence and mortality. However, little is known about how metabolic disease regulates the development and progression of breast cancer at the molecular level. Here, using a hybridization capture-based panel including 124 cancer-associated genes, we performed targeted next-generation sequencing of tumor tissues and matched blood samples from 20 postmenopausal patients with primary breast cancer, in which 6 cases suffered from preexisting metabolic disorders including hypertension, type 2 diabetes, and coronary heart disease. We took only the protein-altering variants and identified 170 somatic mutations of 59 genes. Among these, 40 mutated genes were found in the metabolic disease group, and 33 mutated genes were found in the non-metabolic disease group. Importantly, nonsynonymous mutations of 26 genes (MSH3, BRAF, MLH3, MTOR, DDR2, ALK, etc.) were uniquely present in the metabolic disease group. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes enrichment analysis were performed to investigate biological functions and key pathways of somatic mutations. TP53, PIK3CA, and PTEN were the top three commonly mutated genes at a higher frequency compared with the Cancer Genome Atlas (TCGA) data, and several novel but infrequent mutations in other genes were also found. Although further studies are required to validate these variants, our results are the first to suggest a specific molecular profile of breast cancer with preexisting metabolic disease.

15.
J Vet Sci ; 20(6): e68, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31775195

RESUMO

Viral-encoded microRNAs (miRNAs) have vital roles in the regulation of virus replications and host immune responses. The results of previous studies have indicated that miRNA clusters are involved in the replication and virulence of the pseudorabies virus (PRV), which may potentially lead to immune escape or facilitation of PRV replication. This study's previous research revealed that prv-miR-LLT11a was differentially expressed during PRV infection. The present study's results have demonstrated that prv-miR-LLT11a could significantly inhibit PRV replication. It was further determined that SLA-1 was the target gene of prv-miR-LLT11a, and simultaneously, that overexpression of prv-miR-LLT11a could downregulate the mRNA and protein levels of SLA-1 in a dose-independent manner. Furthermore, the present study also observed that prv-miR-LLT11a can downregulate TAP1 expression. Our findings provide a better understanding of the molecular mechanism involved in the effects of prv-miR-LLT11a on SLA-1 and TAP1 as well as its involvement in immune system evasion of PRV.

16.
Food Sci Nutr ; 7(11): 3471-3479, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31762998

RESUMO

In the current study, the impact of fermentation and freezing/thawing treatment on corn flour was studied. Fermentation revealed an increase (12%) in amylose content, while freezing reflected a loss of amylose. The results of scanning electron microscope (SEM) revealed more grooves, indentations, and the irregular shape of particles. Rapid Visco Analyzer (RVA) exhibited different pasting behavior on the dough. The molecular structure had similar profiles but showed several discernible absorbance at the different wavelengths. Differential scanning calorimetry (DSC) showed an increase in melting temperature range due to fermentation and freezing/thawing treatment attributed to more heterogeneous morphology. Overall, the results of this research showed the insight alterations that induce the changes in corn flour leading to improvement in some properties and it may enhance the acquaintance about the upright revolution in the profile of corn dough and its potential usage in industry and homes.

17.
Food Funct ; 10(11): 7565-7575, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31687719

RESUMO

The aim of this study was to elucidate the effect and the underlying mechanism of glycosaminoglycan from Apostichopus japonicus (AHG) on hepatic glucose production (HGP) in insulin resistant hepatocytes. Insulin resistance was induced with high glucose (HG) for 24 h in primary hepatocytes. The results showed that AHG exhibited hypoglycemic activity at a relatively low concentration (1 µg mL-1) and revealed non-toxic activity to insulin resistant hepatocytes even at 500 µg mL-1 concentration. The HGP test showed that the treatment of AHG (10 µg mL-1) for 3 h decreased HGP by 25% in insulin resistant hepatocytes. Quantitative PCR and western blot analysis revealed that AHG also ameliorated phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase). The data revealed the mechanism of AHG in alleviating HGP via activating the Akt/FoxO1 signaling pathway and suppressing the PKA/CREB signaling pathway in insulin resistant hepatocytes. This finding suggests that AHG could be a potential marine natural product for the treatment of dysregulating glucose homeostasis.

18.
Nanoscale ; 11(48): 23182-23187, 2019 Dec 28.
Artigo em Inglês | MEDLINE | ID: mdl-31777895

RESUMO

There is increasing interest in hot-electron photodetection due to the extended photoresponse well below the semiconductor band edge. However, the photoresponsivity is extremely low and the metallic nanostructures used to excite surface plasmons (SPs) for improved quantum yield are too complex for practical applications. Here, we show that by exciting Tamm plasmons (TPs), a planar device consisting of a thin metal film of 30 nm on a distributed Bragg reflector (DBR) can absorb ∼93% of the incident light, resulting in a high hot-electron generation that is over 34-fold enhanced compared to that of the reference without the DBR. Besides, the electric field increases with the light penetration depth in the metal, leading to hot-electron generation that is strongly concentrated near the Schottky interface. As a result, the photoresponsivity can be over 30 (6) times larger than that of the reference (conventional grating system). Moreover, the planar device exhibits an easily tunable working wavelength from the visible to the near-infrared, sustained performance under oblique incidences, and a multiband photodetection functionality. The proposed strategy avoids the complicated fabrication of the metallic nanostructures, facilitating the compact, large-area, and low-cost photodetection, biosensing, and photocatalysis applications.

19.
Oncol Lett ; 18(5): 5301-5309, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31612040

RESUMO

Factor that binds to the inducer of short transcripts of the human immunodeficiency virus-1 (FBI-1) represents as a crucial gene regulator in colorectal cancer; however, the correlation between FBI-1 and preoperative radiotherapy (RT) in rectal cancer (RC) has not yet been reported. The aim was to detect FBI-1 expression in patients with RC with or without RT, by immunohistochemistry and quantitative polymerase chain reaction, and to analyze its association with clinicopathological features and response to RT. The results from immunohistochemistry analysis (n=139) and reverse transcription-quantitative polymerase chain reaction (n=55) demonstrated that FBI-1 was overexpressed in patients with RC, whether they had received preoperative RT or not. Subsequently, the association between FBI-1 expression, and the clinicopathological features and response to RT in patients with RC was analyzed. Cytoplasmic FBI-1 was upregulated in non-RT (n=77) and RT (n=62) groups (17.7 vs. 74.0%, P<0.001; 41.1 vs. 69.4%, P=0.002, respectively) of patients with RC compared with normal mucosa. However, nuclear FBI-1 was downregulated (75.8 vs. 22.1%, P<0.001; 83.9 vs. 35.5%, P<0.001, respectively) in both groups. RT had no significant effect on FBI-1 expression in RC tissues. Furthermore, nuclear FBI-1 was positively associated with tumor-node-metastasis stage and distant recurrence (P=0.003 and P=0.010, respectively). In patients with stage I, II or III RC, higher nuclear FBI-1 expression was associated with poorer disease-free survival [hazard ratio (HR)=1.934, 95% confidence interval (CI): 1.055-3.579, P=0.033] and overall survival (HR=2.174, 95% CI: 1.102-4.290, P=0.025), independently of sex, age, growth pattern, differentiation and RT. In addition, FBI-1 was positively correlated with numerous biological factors, including p73 [Spearman's correlation coefficient (rs)=0.332, P=0.007], lysyl oxidase (rs=0.234, P=0.043), Wrap53 (rs=-0.425, P=0.0002) and peroxisome proliferator-activated receptor δ (rs=-0.294, P=0.026). In conclusion, the present study demonstrated that nuclear FBI-1 was an independent prognostic factor in patients with RC and correlated with numerous biological factors, which indicated that it may have multiple roles in RC.

20.
Artigo em Inglês | MEDLINE | ID: mdl-31614779

RESUMO

Conspicuous expansion and intensification of impervious surfaces accompanied by rapid urbanization are widely recognized to have exerted evident impacts on the urban thermal environment. Investigating the spatially and temporally varying relationships between Land Surface Temperature (LST) and impervious surfaces (IS) at multiple scales is of great significance for steering IS expansion and intensification. This study proposes an analytical framework to investigate the spatiotemporal variations of LST and its responses to IS in Wuhan, China at both city scale and sub-region scale. The summer LST patterns in 2002-2017 are extracted by Multi-Task Gaussian Process (MTGP) model from raw 8-day synthesized MODerate-resolution Imaging Spectroradiometer (MODIS) LST data. At the city scale, the weighted center of LST (LSTWC) and impervious surface fraction (ISFWC), multi-temporal trajectories and coupling indicators are utilized to comprehensively examine the spatial and temporal dynamics of LST and IS within Wuhan. At the sub-region scale, urban heat island ratio index (URI), impervious surfaces contribution index (ISCI) and sprawl rate are introduced for further quantifying the relationships of LST and IS. The results reveal that IS and hot thermal landscapes expanded by 407.43 km2 and 255.82 km2 in Wuhan in 2002-2017 at city scale. The trajectories of LSTWCs and ISFWCs are visually coherent and both heading to southeast direction in general. At the sub-region scale, the specific cardinal directions with the highest ISCI variations are examined to be the exact directions of ISFWC trajectories in 2002-2017. The results reveal that the spatiotemporal variations of LST and IS are highly correlated at both city and sub-region scales within Wuhan, thus testifying the significance of steering IS expansion and renewal for controlling urban thermal environment deterioration.

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