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1.
Toxicol Lett ; 316: 10-19, 2019 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-31476341

RESUMO

Rapid risk assessment models for different types of cigarette smoke extract (CSE) exposure are critical to understanding the etiology of chronic obstructive pulmonary disease. The present study investigated inflammation of cultured tracheal tissues with CSE exposure. Rat trachea rings were isolated, cultured, then exposed to various concentrations of CSE from 3R4 F reference cigarettes for 4 h. Tissue/cellular morphology, ultrastructure, viability and damage, inflammatory cell infiltration, and inflammatory protein levels were measured and compared to untreated controls. Human bronchial epithelial cells (BEAS-2B) exposed to 0 or 300 µg/mL CSE were cocultured with macrophages to assess extent of mobilization and phagocytosis. Endotracheal epithelium cilia densities were significantly reduced with increasing CSE concentrations, while mucous membranes became increasingly disordered; both eventually disappeared. Macrophages became larger as the CSE concentration increased, with microvilli and extended pseudopodium covering their surface, and many primary and secondary lysosomes present in the cytoplasm. Inflammatory cell infiltration also increased with increasing CSE dose, as did intracellular adhesion molecule-1(ICAM-1), interleukin-6(IL-6). The method described here may be useful to qualitatively characterized the effects of the compound under study. Then, we use BEAS-2B cell line system to strength the observation made in the cultured tissues. Probably, an approach to integrate results from both experiments will facilitate its application. These results demonstrate that cultured rat tracheal rings have a whole-tissue structure that undergoes inflammatory processes similar to in vivo tissues upon CSE exposure.

2.
Atherosclerosis ; 289: 94-100, 2019 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-31487565

RESUMO

BACKGROUND AND AIMS: About 20% of patients with ST-segment elevated myocardial infarction (STEMI) are young adults. Morphological characteristics of culprit lesion in young STEMI patients have not been systematically evaluated in vivo. The present study aimed to investigate culprit lesion characteristics in young patients versus older patients using optical coherence tomography (OCT). METHODS: 1442 STEMI patients who underwent OCT examination of culprit lesion were included and divided into young group (age ≤50 years, n = 400) and older group (age >50 years, n = 1042). Clinical characteristics, angiography and OCT findings were compared between the two groups. RESULTS: Culprit lesions in STEMI patients aged ≤50 years had more plaque erosion (32.0% vs. 21.1%, p < 0.001) and larger minimal lumen area (2.3 ±â€¯1.7 mm2vs. 1.9 ±â€¯1.1 mm2, p < 0.001) than in those aged >50 years. As compared with older patients, lipid rich plaque (80.5% vs. 87.2%, p = 0.001), thin cap fibroatheroma (TCFA, 59.5% vs. 69.5%, p < 0.001), calcification (31.3% vs. 48.7%, p < 0.001), spotty calcification (25.3% vs. 36.1%, p < 0.001) and cholesterol crystals (26.3% vs. 38.4%, p < 0.001) were less frequently observed in young patients. A gradient increase in typical plaque vulnerability was observed from age ≤50 years to 50-70 years to >70 years. In multivariate regression analysis, age ≤50 years was independently associated with less frequency of plaque rupture, TCFA, spotty calcification, cholesterol crystals and smaller lumen area stenosis. CONCLUSIONS: Morphological characteristics of culprit lesion in young STEMI patients were different from those in older patients. Patients aged ≤50 years had more plaque erosion and less vulnerable plaque features.

3.
Ecotoxicol Environ Saf ; 184: 109617, 2019 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-31476449

RESUMO

Cigarette smoking, as an individual consumption habit, is associated with a variety of related diseases. Exposure of cigarette smoke was reported to induce autophagy and inflammation in experimental animals and humans. However, the toxicity mechanism of cigarette smoke in organisms has not been entirely investigated. In this present study, we studied the role of autophagy played in the inflammation caused by cigarette smoke in human bronchial epithelial cells (BEAS-2B), as well as the role of the phosphatidylinositol 3-kinase (PI3K) signaling pathway and the mitogen-activated protein kinase (MAPK) signaling pathways underlying autophagy and inflammation. We found that cigarette smoke induced autophagy and inflammation in BEAS-2B, and the blockage of autophagy significantly reduced the release levels of IL-1ß, IL-6 and IL-8 in BEAS-2B exposed to cigarette smoke for 24 h. Cigarette smoke downregulated the activity of PI3K/Akt/mTOR pathway and elevated the activity of MAPK pathways. Pretreatment of autophagic inhibitor could inhibit autophagy and the activity of JNK and p38 pathways. These results suggested that cigarette smoke-induced autophagy triggered inflammation through the activation of JNK and p38 pathways, which might contribute to understanding the adverse outcome pathways induced by cigarette smoke exposure and provide the information about the risk assessment of tobacco products.

4.
J Infect Dis ; 2019 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-31419286

RESUMO

BACKGROUND: Trans-translation is a ribosome rescue system that plays an important role in bacterial tolerance to environmental stresses. It is absent in animals, making it a potential treatment target. However, its role in antibiotic tolerance in Pseudomonas aeruginosa remains unknown. METHODS: The role and activity of trans-translation during antibiotic treatment were examined with a trans-translation-deficient strain and a genetically modified trans-translation component gene, respectively. In vitro assays and murine infection models were used to examine the effects of suppression of trans-translation. RESULTS: We found that the trans-translation system plays an essential role in P. aeruginosa tolerance to azithromycin and multiple aminoglycoside antibiotics. We further demonstrated that gentamicin could suppress the azithromycin-induced activation of trans-translation. Compared with each antibiotic individually, gentamicin and azithromycin combined increased the killing efficacy against planktonic and biofilm-associated P. aeruginosa cells, including a reference strain PA14 and its isogenic carbapenem-resistance oprD mutant, the mucoid strain FRD1, and multiple clinical isolates. Furthermore, the gentamicin-azithromycin resulted in improved bacterial clearance in murine acute pneumonia, biofilm implant, and cutaneous abscess infection models. CONCLUSIONS: Combination treatment with gentamicin and azithromycin is a promising strategy in combating P. aeruginosa infections.

5.
Aging (Albany NY) ; 11(16): 6089-6108, 2019 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-31442207

RESUMO

Copy number variations (CNVs) are crucial genetic change elements in malignancies, and lncRNAs deregulation induced by genomic and epigenomic aberrations plays key driving role in tumorigenesis, including colorectal cancer (CRC). However, effects of CNVs associated with lncRNAs in CRC is largely unknown. Here, we perform integrative analysis considering messenger RNA expression levels, DNA methylation and DNA copy numbers from 289 cases of CRC specimens. There are five prognostic subtypes of CRC determined by multi-omics integration, and differentially expressed lncRNAs (DE-lncRNAs) are acquired among five subtypes and normal cases. Finally, CNVs pattern matched with DE-lncRNAs reveals a signature including 10 lncRNAs (LOC101927604, LOC105377267, CASC15, LINC-PINT, CLDN10-AS1, C14orf132, LMF1, LINC00675, CCDC144NL-AS1, LOC284454), conspicuously contributing to poor prognosis in CRC, which can be validated in another independent dataset. Together, our research is interested in copy number changes relevant with lncRNAs, not only expending the spectrum of CNVs, but also perfecting the regulation network of lncRNAs in CRC. The main purpose is to provide novel biomarkers for prognostic managements of CRC patients.

6.
J Org Chem ; 84(14): 9151-9160, 2019 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-31273988

RESUMO

A selective catalytic system for the dehydrogenation of primary alcohols to carboxylic acids using a facial ruthenium complex generated in situ from the [Ru(COD)Cl2]n and a hybrid N-heterocyclic carbene (NHC)-phosphine-phosphine ligand (CPP) has been first reported. The facial coordination model was unveiled by NMR analysis of the reaction mixture. Such a fac-ruthenium catalyst system exhibited high catalytic activity and stability, and a high turnover number of 20 000 could be achieved with catalyst loading as low as 0.002 mol %. The exceedingly high catalyst stability was tentatively attributed to both the anchoring role of NHC and the hemi-lability of phosphines. The catalytic system also features a wide substrate scope. In particular, the facial coordination of CPP ligands was found to be beneficial for sterically hindered alcohols, and ortho-substituted benzylic alcohols and bulky adamantanyl methanol as well as cholesterol were all found to be viable dehydrogenation substrates.

7.
Artigo em Inglês | MEDLINE | ID: mdl-31309678

RESUMO

Enormous efforts have been devoted to carbon dioxide (CO2) reduction by utilizing various driving forces, such as heat, electricity and radiation. However, efficient reduction of CO2 is still challenging because of the sluggish kinetics. Recent pioneering works from several groups including us have demonstrated that coupling of solar energy and thermal energy serves as a novel and promising strategy to promote activity and/or manipulate selectivity in CO2 reduction. Herein, we will clearly clarify the definition and principles of coupling of solar energy and thermal energy, and comprehensively review the status and prospects of CO2 reduction by coupling of solar energy and thermal energy. Catalyst design, reactor configuration, photo-mediated activity/selectivity and mechanism studies in photo-thermo CO2 reduction will be emphasized. This review aims to promote the understandings towards CO2 activation and provide guidelines for the design of new catalysts for efficient CO2 reduction.

8.
Food Funct ; 10(6): 3603-3614, 2019 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-31161181

RESUMO

Ginsenoside Rg2 is one of the specific ginsenosides in red ginseng, and has been reported to exhibit protective effects against neurotoxicity and memory impairment, and also inhibition of hepatic glucose production. However, the effect of Rg2 on the prevention of obesity has not been investigated. In this study, we evaluated the anti-obesity and anti-adipogenic effects of Rg2 in high-fat diet-induced obese mice (HFD mice) and 3T3-L1 preadipocytes. Oral administration of Rg2 (10 mg kg-1) to HFD mice significantly decreased body weight gain, total triglycerides, and free fatty acid levels. In 3T3-L1 preadipocytes, Rg2 (80 µM) inhibited adipocyte differentiation and reduced the accumulation of intracellular lipids. Quantitative PCR and western blot analysis revealed that Rg2 decreased the expression levels of adipogenic transcription factors (PPARγ, C/EBPα, and SREBP1-c), and then regulated target genes such as acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS). Rg2 significantly promoted AMP-activated protein kinase (AMPK) both in vivo and in vitro, which is known to suppress adipogenesis. It was also found that pretreating with compound C, a typical inhibitor of AMPK, attenuated the inhibitory effect of Rg2 on AMPK phosphorylation. These findings suggested that Rg2-induced activation of AMPK leads to a decrease in the expression of adipogenic transcription factors, and suppression of adipogenesis in vivo and in vitro. Hence, Rg2 has the potential for the development of healthy foods and the prevention of obesity.

9.
J Mol Endocrinol ; 2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-31247591

RESUMO

The prevalence of obesity is dramatic increased and strongly associated with cardiovascular disease. Adipokines, secreted from adipose tissues, are critical risk factors for the development of cardiomyopathy. Present study aimed to investigate the pathophysiological role of autotaxin in obesity-related cardiomyopathy. In high fat diet-fed mice, autotaxin was mainly synthesized and secreted from adipocytes. The increased accumulation of cardiac autotaxin was positively associated with cardiac dysfunction in obese mice. Interestingly, specific blockage of adipose tissue autotaxin effectively protected against high fat diet-induced cardiac structural disorders, left ventricular hypertrophy and dysfunction. Inhibition of autotaxin further improved high fat diet-induced cardiac fibrosis and mitochondrial dysfunction, including improvement of mitochondrial structure, mass and activities. Our findings demonstrated intervention of adipose tissue biology could influence cardiac modification in obese mice, and adipocyte-derived autotaxin was a potential diagnostic marker and therapeutic target for obesity-related cardiomyopathy.

10.
J Dairy Sci ; 102(8): 6914-6919, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31202653

RESUMO

Escherichia coli has been frequently reported as a major foodborne bacterium contaminating raw milk or pasteurized milk. Therefore, the aim of this study was to explore a quantitative real-time PCR (qPCR) technique combined with sodium dodecyl sulfate (SDS) and propidium monoazide (PMA) to detect culturable E. coli in milk. An internal amplification control was also added into this reaction system as an indicator of false-negative results. The inclusivity and exclusivity of the primers were tested using DNA from 7 E. coli and 14 other bacterial strains. The concentrations of SDS and PMA were determined according to plate counts and quantitative cycle values of qPCR, respectively. A standard curve was established using series diluted E. coli DNA. The reliability and specificity of this method were further determined by the detection of E. coli in spiked milk. The results showed that the optimal concentrations of SDS and PMA were 100 µg/mL and 40 µM, respectively. A standard curve with a good linear relationship (coefficient of determination = 0.997; amplification efficiency = 100.5%) was obtained. Compared with conventional PCR and PMA-qPCR, the SDS-PMA-qPCR assay was more specific and sensitive in culturable E. coli detection. Therefore, we evaluated and improved the SDS-PMA-qPCR method for detecting culturable E. coli in milk.

11.
Acta Diabetol ; 2019 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-31115753

RESUMO

AIMS: Hyperglycemia induces endothelial cell apoptosis and blood vessel damage, while diallyl trisulfide (DATS) has shown cardiovascular protection in animal models and humans. The aim of this study was to investigate the effects of DATS on inhibition of high glucose-induced endothelial cell apoptosis and the underlying molecular events. METHODS: Human umbilical vein endothelial cells (HUVECs) were incubated with DATS (100 µM) for 30 min and then cultured in high-glucose medium (HG, 33 mM) for 24 h for assessment of apoptosis, glutathione (GSH), reactive oxygen species (ROS), superoxide dismutase (SOD), and gene expression using the terminal deoxyuridine triphosphate nick end labeling (TUNEL), flow cytometry, caspase-3 activity, ROS, SOD, and western blot assays as well as JC-1 and MitoTracker Red staining, respectively. RESULTS: DATS treatment significantly inhibited high glucose-induced HUVEC apoptosis by blockage of intracellular and mitochondrial ROS generation, maintenance of the mitochondrial membrane potential, and suppression of high glucose-induced dynamin-related protein 1 (Drp1) expression. Furthermore, DATS blockage of high glucose-induced mitochondrial fission and apoptosis was through adenosine monophosphate-activated protein kinase (AMPK) activation-inhibited Drp1 expression in HUVECs. CONCLUSIONS: DATS demonstrated the ability to inhibit high glucose-induced HUVEC apoptosis via suppression of Drp1-mediated mitochondrial fission in an AMPK-dependent fashion.

12.
Anal Chem ; 91(12): 7842-7849, 2019 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-31121095

RESUMO

A fiber Bragg grating (FBG) was created to accurately and simultaneously monitor the biohydrogen and metabolic heat production in biofilms containing Rhodopseudomonas palustris CQK-01 photosynthetic bacteria (PSB). The proposed hydrogen sensor was made from an FBG unit separated into two regions by a wet etching process; a thin region with a diameter of 15 µm was employed to monitor the temperature. A smaller region of the etched FBG with a diameter of 8.0 µm was coated with a 50 nm-thick Pd film by sputtering to determine the responses to the temperature and hydrogen concentration. To monitor the biohydrogen production and metabolic heat within the biofilms, three FBGs were evenly distributed in a polydimethylsiloxane channel (biofilm carrier) with vertical distances of 80 µm. In addition, the thickness, surface morphology, active biomass, and porosity of the biofilms were investigated. The FBG sensor can rapidly and accurately determine the difference in Bragg wavelength shifts caused by changes in the hydrogen concentration and temperature. The measured biohydrogen concentration is highly correlated with the real biohydrogen production with a correlation of 0.9765. The biohydrogen production capacity of PSB in the surface layer is much higher than that internally because of sharp decreases in the active biomass and porosity from the surface to within the biofilm. The highest biohydrogen concentration is obtained at 1.218 × 104 ppm for a biofilm thickness of 165 µm, and the temperature difference from metabolic heat production is ∼1.1 °C in the biofilm culture.

13.
Bioorg Chem ; 88: 102940, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31028991

RESUMO

A series of imidazo[4,5f][1,10]phenanthroline derivatives (1-6) have been synthesized in this study, and their inhibitory activity was evaluated by MTT assay. Results showed that all of these compounds demonstrate a promising inhibitory activity against a panel of human cancer cell lines. The 6, the most effective compound with IC50 of approximately 2.3 ±â€¯0.1 µM, was against the growth and could induce autophagy of HepG2 cells. This condition was confirmed by abundant autophagic vacuoles appearing in cells and evident ultrastructural changes observed under transmission electron microscopy. The autophage induced by 6 has also been demonstrated by up-regulating LC3-II and Beclin1. The apoptosis and G2/M phase cell cycle arrest through DSB damage have also been confirmed after the HepG2 cells were treated by 6. These multiple effects, especially induction apoptosis and autophagy, indicate the potential of 6 for development as a novel anticancer drug.

14.
Dis Markers ; 2019: 5231269, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30956730

RESUMO

Colorectal cancer (CRC) is a complex and heterogeneous disease with four consensus molecular subtypes (CMS1-4). LTBP2 is a member of the fibrillin/LTBP super family and plays a critical role in tumorigenesis by activating TGF-ß in the CMS4 CRC subtype. So far, the expression and prognostic significance of LTBP2 in CRC remains obscure. In this study, we aimed to analyze the mRNA and protein expression levels of LTBP2 in CRC tissues and then estimate their values as a potential prognostic biomarker. We detected the mRNA expression of LTBP2 in 28 cases of fresh CRC tissues and 4 CRC cell lines and the protein expression of LTBP2 in 483 samples of CRC tissues, matched tumor-adjacent tissues, and benign colorectal diseases. LTBP2 protein expression was then correlated to patients' clinical features and overall survival. Both LTBP2 mRNA and protein expression levels in CRC tissues were remarkably superior to those in adjacent normal colorectal tissues (P = 0.0071 and P < 0.001, respectively), according to TCGA dataset of CRC. High LTBP2 protein expression was correlated with TNM stage (P < 0.001), T stage (P < 0.001), N stage (P < 0.001), and M stage (P < 0.001). High LTBP2 protein expression was related to poor overall survival in CRC patients and was an independent prognostic factor for CRC. LTBP2 mRNA expression was especially higher in the CMS4 subtype (P < 0.001), which was confirmed in CRC cell lines. Our data suggested that LTBP2 may act as an oncogene in the development of colorectal cancer and have important significance in predicting CRC prognosis. LTBP2 could be a novel biomarker and potential therapeutic target for mesenchymal colorectal cancer and can improve the outcome of high-risk CRC.


Assuntos
Biomarcadores Tumorais/genética , Neoplasias Colorretais/genética , Proteínas de Ligação a TGF-beta Latente/genética , Biomarcadores Tumorais/metabolismo , Células CACO-2 , Neoplasias Colorretais/classificação , Neoplasias Colorretais/metabolismo , Neoplasias Colorretais/patologia , Transição Epitelial-Mesenquimal , Feminino , Células HT29 , Humanos , Proteínas de Ligação a TGF-beta Latente/metabolismo , Masculino , Pessoa de Meia-Idade , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Regulação para Cima
15.
Sensors (Basel) ; 19(8)2019 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-30999637

RESUMO

The Internet of Things (IoT) has tremendous success in health care, smart city, industrial production and so on. Protected agriculture is one of the fields which has broad application prospects of IoT. Protected agriculture is a mode of highly efficient development of modern agriculture that uses artificial techniques to change climatic factors such as temperature, to create environmental conditions suitable for the growth of animals and plants. This review aims to gain insight into the state-of-the-art of IoT applications in protected agriculture and to identify the system structure and key technologies. Therefore, we completed a systematic literature review of IoT research and deployments in protected agriculture over the past 10 years and evaluated the contributions made by different academicians and organizations. Selected references were clustered into three application domains corresponding to plant management, animal farming and food/agricultural product supply traceability. Furthermore, we discussed the challenges along with future research prospects, to help new researchers of this domain understand the current research progress of IoT in protected agriculture and to propose more novel and innovative ideas in the future.


Assuntos
Agricultura/tendências , Redes de Comunicação de Computadores , Abastecimento de Alimentos , Internet/tendências , Cidades , Humanos , Tecnologia sem Fio
16.
Food Funct ; 10(4): 2221-2233, 2019 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-30950462

RESUMO

Zeaxanthin (ZEA), a type of oxygenated carotenoid with strong antioxidant activity, has previously been found to exhibit an anti-lipogenesis effect. In the present study, we investigated the effect of ZEA on brown-like adipocyte formation and mitochondrial biogenesis in 3T3-L1 adipocytes. Brown adipocyte-specific markers, mitochondrial biogenesis and oxidative stress, and the involvement of AMP-activated protein kinase (AMPK) α1 were assessed. ZEA treated adipocytes demonstrated a brown-like pattern, with upregulated expression of uncoupling protein 1 (UCP1) and other brown adipocyte markers. In addition, ZEA intervention induced a dramatic increase in mitochondrial DNA (mtDNA) content and in the mRNA levels of genes associated with mitochondrial biogenesis. Furthermore, ZEA attenuated mitochondrial oxidative damage caused by lipid peroxidation in adipocytes, significantly improved the mitochondrial membrane potential (MMP), and scavenged intracellular reactive oxygen species (ROS) and mitochondrial superoxide. Finally, we concluded that AMPKα1 mediated the ZEA-caused inhibition of lipid accumulation and promotion of brown and beige adipocyte-biomarker expression, as the positive effects of ZEA were diminished by Prkaa1 (AMPKα1) knockdown. These findings demonstrated that ZEA promoted the expression of brown and beige adipogenesis markers and mitochondrial biogenesis, which involved AMPKα1 activation, thus contributing to the anti-obesity effects of ZEA.


Assuntos
Proteínas Quinases Ativadas por AMP/metabolismo , Adipócitos Marrons/efeitos dos fármacos , Mitocôndrias/efeitos dos fármacos , Zeaxantinas/farmacologia , Células 3T3-L1 , Proteínas Quinases Ativadas por AMP/genética , Adipócitos Bege/efeitos dos fármacos , Adipócitos Bege/metabolismo , Adipócitos Marrons/metabolismo , Adipogenia , Animais , Biomarcadores/metabolismo , Camundongos , Mitocôndrias/genética , Mitocôndrias/metabolismo , Biogênese de Organelas
17.
Appl Opt ; 58(8): 2091-2099, 2019 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-30874074

RESUMO

A photochemical fiber-optic sensor was developed by integrating a plastic optical fiber (POF), polymer membrane, gold mirror, and TiO2-based composite, and was shown to sensitively and selectively detect phenol in aqueous solution. The sensing element consisted of a thinned POF and visible-light-driven SiO2/N-doped TiO2 coating. The gold mirror was used to develop a reflective POF probe. The polymer membrane with high phenol permselectivity was employed to form a micro-channel between the membrane and probe. Our findings highlight the sensor's capability of phenol detection in aqueous solutions with high sensitivity of 0.294×10-3 (mg·L-1)-1, pH immunity ranging from 2.0 to 14.0, and high selectivity with a limit of detection of 30 µg·L-1.

18.
J Plant Physiol ; 234-235: 154-166, 2019 Mar - Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30818185

RESUMO

This paper investigates the physiological mechanism of the effect of delaying cucumber senescence on protein levels under the experimental model of monoculture and wheat intercropping. We analyzed cucumber roots for differential protein expression, and protein types were identified by core technology of proteomics. 45 differential proteins were identified as being differentially expressed between monoculture and intercropping of wheat, which were involved in carbohydrate metabolism, antioxidant and stress response, growth and development regulation, biological information transfer, and nucleic acid biosynthesis. The results showed the rate of photosynthesis of cucumber was increased under wheat intercropping pattern due to three enzymes being up-regulated. The respiration of cucumber was reduced when five enzymes were down-regulated. The antioxidant and resistant capacity of cucumber was enhanced significantly under wheat intercropping pattern because two enzymes were down-regulated while the activity of four other antioxidant enzymes was up-regulated. Intercropping wheat could delay the senescence of cucumber leaves by increasing the expression of IPT gene, reducing the expression of PAO and ETR2 gene, and inhibiting the expression of Cu/Zn-SOD and GS1 gene in later stages. Two proteins related to growth and development in cucumber were up-regulated, and one was down-regulated, while two proteins related to nucleic acid biosynthesis and chaperonin showed obvious down-regulation under wheat intercropping. Therefore, the growth and development was improved and senescence of cucumber could be delayed. Under intercropping pattern, the fresh weight, chlorophyll content, photosynthetic rate, and peroxidase activity of cucumber plants were higher than those of cucumber monoculture groups. Thus, the senescence of cucumber could be delayed under wheat intercropping by regulating its physiological mechanisms, such as by improving photosynthesis, reducing respiratory consumption, slowing the cell apoptosis rate, and enhancing the antioxidant and resistant capacity significantly, etc.


Assuntos
Cucumis sativus/fisiologia , Proteínas de Plantas/metabolismo , Raízes de Plantas/metabolismo , Agricultura/métodos , Folhas de Planta/fisiologia , Triticum
19.
BMC Cancer ; 19(1): 237, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30876464

RESUMO

BACKGROUND: Patients with ovarian cancer commonly have a poor prognosis, owing to its invasiveness and distant metastasis. Studies have found TM4SF1 participates in regulating tumor cell invasion and migration. Therefore, it is expected to become a target for anti-invasion and metastasis in ovarian cancer. METHODS: The expression of TM4SF1 in normal ovarian epithelial tissues, benign ovarian tumor tissues, primary foci of epithelial ovarian cancer and the matched lymph mode metastatic foci was detected using immunohistochemistry to analyze its association with prognosis. The expression of TM4SF1 in HO8910PM, SKOV3 was inhibited using RNAi, and the growth, proliferation, migration, invasion abilities of HO8910PM and SKOV3 cells and the growth of xenograft tumors in nude mice were examined. RESULTS: (1) The positive expression rate of TM4SF1 protein in epithelial ovarian cancer tissues (90.90%) was higher than that in benign ovarian tumor tissues (65.22%) and normal ovarian epithelial tissues (31.25%), and both differences were significant (P < 0.05). The expression of TM4SF1 protein was positive in all metastatic lymph node foci and matched primary foci (100%). (2) The level of TM4SF1 protein expression was positively correlated with the International Federation of Gynecology and Obstetrics (FIGO) stage and histological grade. However, The positive TM4SF1 protein expression was not an independent factor of prognosis (P > 0.05). (3) Silencing TM4SF1 expression did not affect growth, proliferation, or cell cycle distribution but inhibited the migration and invasion abilities of HO8910PM and SKOV3 cells. Silencing TM4SF1 expression inhibited the growth of xenograft tumors in nude mice. CONCLUSION: TM4SF1 is a potential target for anti-invasion and metastasis in ovarian cancer.


Assuntos
Antígenos de Superfície/metabolismo , Carcinoma Epitelial do Ovário/patologia , Proteínas de Neoplasias/metabolismo , Neoplasias Ovarianas/patologia , Regulação para Cima , Animais , Carcinoma Epitelial do Ovário/metabolismo , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Camundongos , Camundongos Nus , Gradação de Tumores , Invasividade Neoplásica , Metástase Neoplásica , Proteínas de Neoplasias/antagonistas & inibidores , Estadiamento de Neoplasias , Transplante de Neoplasias , Neoplasias Ovarianas/metabolismo , Prognóstico , Interferência de RNA/fisiologia
20.
J Agric Food Chem ; 67(13): 3651-3661, 2019 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-30875211

RESUMO

Forkhead/winged helix transcription factor O-class member 1 (FoxO1) is a key mediator of insulin and glucagon signaling in control of glucose homeostasis. Although epigallocatechin gallate (EGCG) has attracted interest owing to its potential to combat hyperglycemic diabetes, molecular mechanisms underlying its antihyperglycemic effect, in particular the effect on FoxO1, is poorly understand. This study aims to assess the impact of EGCG on the glucagon signaling pathway in regulating glucose metabolism. Primary hepatocytes from wild-type (WT), liver-specific FoxO1 knock out (FKO), and FoxO1-S273D knock-in (KI) mice were isolated, cultured, and treated with EGCG and/or glucagon. Our data showed the treatment of 10 µM EGCG for 6 h decreased hepatic glucose production by 20 and 23% in WT and FKO primary hepatocytes, respectively. EGCG repressed both gluconeogenesis and glycogenolysis in primary hepatocytes, coupled with activating AMPK. In addition, EGCG decreased mitochondrial oxygen consumption. We further investigated the effects of EGCG on glucagon-stimulated cAMP/PKA signaling pathway. EGCG reduced p-PKA-T197/T-PKA and p-CREB-S133/T-CREB levels by 39 and 20%, blocked p-FoxO1-S273, and suppressed nuclear FoxO1 translocation, suggesting that FoxO1 and CREB were possible downstream targets. A novel mechanism of EGCG in restraining hepatic glucose production (HGP) is through antagonizing glucagon signaling and suppressing FoxO1 via Ser273. EGCG may serve as a promising compound for regulating glucose homeostasis.


Assuntos
Catequina/análogos & derivados , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Proteína Forkhead Box O1/metabolismo , Glucose/metabolismo , Hepatócitos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Animais , Catequina/farmacologia , Proteínas Quinases Dependentes de AMP Cíclico/genética , Regulação para Baixo/efeitos dos fármacos , Proteína Forkhead Box O1/genética , Hepatócitos/metabolismo , Fígado/metabolismo , Camundongos , Camundongos Knockout , Transdução de Sinais/efeitos dos fármacos
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