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1.
Int J Hyg Environ Health ; 223(1): 171-178, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31548162

RESUMO

AIMS: Evidence concerning the impact of ambient particulate matter (PM) on mental health is just emerging and inconsistent. Air pollution with high PM levels has been frequently reported in China, however, no Chinese study has determined the association between PM exposures and anxiety hospitalizations. We examined the potential association between PM concentrations and anxiety admissions in 26 Chinese cities from January 2014 to December 2015. METHODS: A time-stratified case-crossover design was employed in the study. Anxiety hospitalizations were identified according to ICD-10 from the electronic hospitalization summary reports system in China. Conditional logistic regression was applied to estimate the relation between PM levels and anxiety admissions, stratified by age and sex. RESULTS: Positive associations between PM2.5/PM10 and admitted anxiety cases were observed. PM2.5 had the largest effect estimate at lag 5 days, with a per 10 µg/m3 increase corresponding to a 0.63% (95% CI, 0.26-1.00) increase in anxiety admissions. PM10's largest effect estimate was observed at lag 3 days, increasing 0.37% (95% CI, 0.12-0.62) anxiety admissions per 10 µg/m3. Females were more sensitive to PM2.5/PM10 concentrations than males, however, the effect modification by age was not significant. A marginally significant distinction in anxiety hospitalizations was found in patients with and without CVDs when they were exposed to PM2.5. CONCLUSIONS: Our findings indicate that short-term exposure to increased concentrations of PM2.5/PM10 exacerbates risks of anxiety hospitalizations in 26 Chinese cities. We observed effect modification by sex, with significantly stronger associations in female patients. This study offers the promise that reducing PM air pollution could probably reduce the huge disease burden from anxiety disorders.

2.
Sci Total Environ ; 700: 134414, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31698277

RESUMO

This study synthesized magnetic greigite/biochar composites (MGBs) by a solvothermal method and tested their ability to remove Cr(VI) from heavy metal-polluted wastewater. X-ray diffraction (XRD), Fourier transformed infrared spectrometry (FT-IR) and scanning electron microscopy (SEM) revealed that magnetic greigite (Fe3S4) flakes were aggregated and anchored to the biochar surface, resulting in more active sites than pristine biochar. Maximum Cr removal efficiency and capacity of MGB-30 (greigite/biochar = 30%) at an initial Cr(VI) concentration of 20 mg/L were 93% and 23.25 mg/g, respectively. A pseudo-first-order kinetic model was determined for the Cr(VI) removal process and the Cr(VI) removal rate constants were highly dependent on the mass ratios of Fe3S4 loaded on biochar, initial MGB and Cr(VI) concentrations and solution pH. X-ray photoelectron spectroscopy (XPS) and flame atomic absorption spectrometric (FAAS) analysis demonstrated that Cr(VI) was preferentially adsorbed on MGBs and subsequently reduced to Cr(III) by MGBs. Electron paramagnetic resonance (EPR) spectroscopy and iron redox transformations revealed that the Cr(VI) removal enhancement was attributed to efficient surface Fe(III)/Fe(II) cycling via electron transfer with the persistent free radicals (PFRs) of biochar. These novel findings provide new insights into the Fe(III)/Fe(II) cycle induced by biochar and the prospects of using magnetic greigite/biochar composites for remediation of Cr(VI)-rich wastewaters.

3.
Ecotoxicol Environ Saf ; 187: 109855, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31689622

RESUMO

A calcium precipitating and denitrifying bacterium H12 was used to investigate the F- removal performance and mechanism. The results showed that the strain H12 reduced 85.24% (0.036 mg·L-1·h-1) of F-, 62.43% (0.94 mg·L-1·h-1) of Ca2+, and approximately 100% of NO3- over 120 h in continuous determination experiments. The response surface methodology analysis demonstrated that the maximum removal efficiency of F- was 88.98% (0.062 mg·L-1·h-1) within 72 h under the following conditions: the initial Ca2+ concentration of 250.00 mg·L-1, pH of 7.50, and the initial C4H4Na2O4·6H2O concentration of 800.00 mg·L-1. The scanning electron microscopy images, the X-ray photoelectron spectroscopy, and X-ray diffraction results suggested the following removal mechanism of F-: (1) the bacteria, as the nucleation site, were encapsulated by bioprecipitation to form biological crystal seeds; (2) Biological crystal seeds adsorbed F- to form Ca5(PO4)3F and CaF2; (3) Under the induction of bacteria, calcium, fluoride and phosphate coprecipitated to form Ca5(PO4)3F and CaF2. In addition, the gas chromatography data indicated that F- had little or no effect on the gas composition during denitrification, and the fluorescence spectroscopy analysis also proved that the extracellular polymeric substance (protein) is the site of bioprecipitation nucleation.

4.
Toxicol In Vitro ; 62: 104667, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31629901

RESUMO

Serum is an important component in cell culture medium. It also possesses potent antioxidant properties. Therefore, the conventional protocols for detecting reactive oxygen species (ROS) in cultured cells with fluorescent probes include washing and suspending cells with serum-free buffers, such as PBS. This transient serum deprivation is essential for the ROS detecting. Unfortunately, it may also cause unexpected results, which push us to choose more optimal experiment conditions. In the present study, we found an acute lytic cell death induced by xanthohumol (XN), which obstructed ROS detecting in human leukemia cell line HL-60 cells. XN induced ROS burst, caused cell swelling, membrane permeability increase, LDH release, and ultimately an acute lytic cell death and cell rupture. These effects could be alleviated by the antioxidant N-Acetyl-L-cysteine (NAC). Apoptosis, pyroptosis or necroptosis were not observed in this process. Results also indicated that 2% serum addition had already completely scavenged ROS induced by 10 µM XN. Taken together, it is strongly suggested to detecting ROS in a serum-free medium when studying where and how ROS generated in cells. The concentration at the ROS maximum point (10 µM XN in this study) can be selected as the optimal concentration.

5.
Food Chem ; 308: 124832, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-31648093

RESUMO

Potatoes usually suffer from greatly decrease of hardness after boiling, which limits their processing potential in food industry. Moreover, methods for enhancing the hardness of potatoes after boiling are underexplored. In this study, the hardness of potato slices after boiling were increased from 288 g to 2342 g by the combined treatment of lactic acid (LA) and calcium chloride (CC). Through the analysis of the microstructure of the potato cells, the molecular weight distribution and natural sugar ratio of different soluble pectin fractions, and the enzymatic activities (polygalacturonase, PG and pectin methylesterase, PME), the possible mechanism behind the hardness enhancement by LA and CC pretreatment, namely the direct link between pectin and potato structure was revealed. The obtained results confirmed the target spot for enhancing the hardness of potatoes after boiling lay in PG activity and gelation of the pectin, which also could be used to help other plants resist the heat process if pectin existed in their cell wall.

6.
J Nanosci Nanotechnol ; 20(5): 2878-2886, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31635624

RESUMO

Magnetic Ni0.3Mg0.3Zn0.4Fe2O4 nanoparticles were synthesized by the absolute alcohol combustion method. The morphology, microstructure, and composition of as-prepared Ni0.3Mg0.3Zn0.4Fe2O4 nanoparticles were characterized by several techniques: the vibrating sample magnetometer (VSM), the scanning electron microscopy (SEM), the X-ray diffraction (XRD), and the energy dispersive spectroscopy (EDS). The experimental results showed that the calcination temperature and the solvent volume were the crucial factors for the synthesis of the magnetic Ni0.3Mg0.3Zn0.4Fe2O4 nanoparticles. The adsorption performance of Ni0.3Mg0.3Zn0.4Fe2O4 nanoparticles for congo red (CR) was investigated. The model of pseudo-second-order kinetic was optimal matching for obtaining the parameters of adsorption CR in the initial range of 100-400 mg/L-1, while, the isotherm data of CR onto Ni0.3Mg0.3Zn0.4Fe2O4 nanoparticles could conform to the Temkin model owing to the values of the square deviations, which revealed that the adsorption of CR onto Ni0.3Mg0.3Zn0.4Fe2O4 nanoparticles at room temperature was the monolayer and multilayer adsorption mechanism.

7.
J Nanosci Nanotechnol ; 20(5): 2998-3003, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31635639

RESUMO

A novel type of magnetic Fe2O3/Fe3O4 heterogeneous microparticles with ellipsoidal macropores was prepared via the ethanol-water reflux and rapid combustion process. The experimental results showed that the volume of absolute ethyl alcohol and the calcination temperature were the key factors to the grain sizes and the magnetic properties, the calcination temperature largely affected the saturation magnetization and the grain size of Fe2O3/Fe3O4 heterogeneous microparticles, and the amount of absolute ethyl alcohol also tremendously affected the saturation magnetization, however, the amount of absolute ethyl alcohol affected little on the grain size. Fe2O3/Fe3O4 heterogeneous microparticles calcined at 200 °C for 1 h with absolute ethyl alcohol of 20 mL had the largest saturation magnetization of 90.1 Am²/kg.

8.
Br J Radiol ; : 20190615, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31794250

RESUMO

OBJECTIVES: To review safety and efficacy of percutaneous microwave ablation (MWA) for post-procedural haemostasis. METHODS: Institutional databases retrospective research identified 10 cases of iatrogenic bleeding who underwent percutaneous microwave ablation for post-procedural haemostasis. Ultrasound examination with Doppler and contrast enhancement identified a source of active bleeding prior to ablation; additionally they were used as guiding modality for antenna insertion whilst, post ablation, assessed the lack of active extravasation. Target locations included liver intercostal space spleen and thyroid gland. Technical success was defined as positioning of the antenna on the desired location. Treatment endpoint was considered the disappearance of active extravasation in both Doppler imaging and CEUS. RESULTS: Technical success (i.e., positioning of the antenna on the desired location) was achieved in all cases. No complications were noted. All patients post microwave ablation remained hemodynamically stable with no need for transfusion and were discharged from the hospital the next morning. Imaging and clinical follow-up in all patients before exiting the hospital did not depict any sign of active extravasation or bleeding. CONCLUSION: Our limited experience reports preliminary data showing that microwave ablation could be added in the armamentarium of percutaneous therapies for iatrogenic bleeding. More prospective studies with larger patient samples are necessary for verification of this technique as well as for drawing broader conclusions in order to evaluate the place of percutaneous ablation in the treatment algorithm of haemorrhage. ADVANCES IN KNOWLEDGE: Percutaneous ablation might have a role in hemostasis in well selected cases.

9.
Biomolecules ; 9(12)2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31795133

RESUMO

Rheumatoid arthritis (RA) is a known chronic autoimmune disease can cause joint deformity and even loss of joint function. Fibroblast-like synoviocytes (FLS), one of the main cell types in synovial tissues of RA patients, are key effector cells in the development of RA and are considered as promising therapeutic targets for treating RA. Herbal medicines are precious resources for finding novel agents for treating various diseases including RA. It is reported that induction of apoptosis in FLS is an important mechanism for the herbal medicines to treat RA. Consequently, this paper reviewed the current available references on pro-apoptotic effects of herbal medicines on FLS and summarized the related possible signal pathways. Taken together, the main related signal pathways are concluded as death receptors mediated apoptotic pathway, mitochondrial dependent apoptotic pathway, NF-κB mediated apoptotic pathways, mitogen-activated protein kinase (MAPK) mediated apoptotic pathway, endoplasmic reticulum stress (ERS) mediated apoptotic pathway, PI3K-Akt mediated apoptotic pathway, and other reported pathways such as janus kinase/signal transducers and activators of transcription (JAK-STAT) signal pathway. Understanding the apoptosis induction pathways in FLS of these herbal medicines will not only help clear molecular mechanisms of herbal medicines for treating RA but also be beneficial for finding novel candidate therapeutic drugs from natural herbal medicines. Thus, we expect the present review will highlight the importance of herbal medicines and its components for treating RA via induction of apoptosis in FLS, and provide some directions for the future development of these mentioned herbal medicines as anti-RA drugs in clinical.

10.
Biosci Biotechnol Biochem ; : 1-12, 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31790634

RESUMO

Two kinds of Kunitz-type protease inhibitors, AKPI1 and AKPI2, were purified from Apios americana tubers by four steps of column chromatographies and their cDNA cloning was performed. AKPI1 cDNA consist of 809 nucleotides, and the matured protein had 190 amino acids with 20,594 Da. AKPI2 cDNA consist of 794 nucleotides, and the matured protein had 177 amino acids with 19,336 Da. P1 site of AKPI2 was Leu88, suggested the target enzyme was chymotrypsin. On the other hand, Gly85-Ile86-Ser87 was positioned around P1 site of AKTI1. Sequence analysis suggested that two forms (single-chain and two-chain form) of AKPI2 protein were present in the tubers. Recombinant AKPI2 expressed by E.coli system showed inhibitory activity toward serine proteases and heat stability. The Ki values toward chymotrypsin and trypsin were 4 × 10-7 M and 6 × 10-6 M, respectively.Abbreviations: AAL: Apios americana lectin; AATI: Apios americana Bowman-Birk type trypsin inhibitor; ACE: angiotensin-converting enzyme; IPTG: isopropyl-ß-D-thio-galactopyranoside; Ki: inhibition constant; KPIs: Kunitz-type protease inhibitors; L-BAPA: Benzoyl-L-arginine p-nitroanilide monohydrochloride; L-BTPA: Benzoyl-L-tyrosine p-nitroanilide; PFLNA: Pyr-Phe-Leu-p-nitroanilide; RP-HPLC: reverse-phase high-performance liquid chromatography; RT-PCR: reverse transcription-polymerase chain reaction; SDS-PAGE: sodium dodecyl sulfate-polyacrylamide gel electrophoresis; SLIC: sequence and ligation independent cloning; STANA: N-Succinyl-Ala-Ala-Ala-p-nitroanilide; SHR: spontaneously hypertensive rats; TFA: trifluoroacetic acid; UTR: untranslated region.

11.
Sci Data ; 6(1): 295, 2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31784528

RESUMO

The data presented here are related to the studyforrest project that uses the movie 'Forrest Gump' to map brain functions in a real-life context using functional magnetic resonance imaging (fMRI). However, neural-related fMRI signals are often small and confounded by various noise sources (i.e., artifacts) that makes searching for the signals induced by specific cognitive processes significantly challenging. To make neural-related signals stand out from the noise, the audio-visual movie watching fMRI dataset from the project was denoised by a combination of spatial independent component analysis and manual identification of signals or noise. Here, both the denoised data and the labeled decomposed components are shared to facilitate further study. Compared with the original data, the denoised data showed a substantial improvement in the temporal signal-to-noise ratio and provided a higher sensitivity in subsequent analyses such as in an inter-subject correlation analysis.

12.
Mol Cancer ; 18(1): 174, 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31791342

RESUMO

BACKGROUND: Long noncoding RNAs (lncRNAs) play nonnegligible roles in the epigenetic regulation of cancer cells. This study aimed to identify a specific lncRNA that promotes the colorectal cancer (CRC) progression and could be a potential therapeutic target. METHODS: We screened highly expressed lncRNAs in human CRC samples compared with their matched adjacent normal tissues. The proteins that interact with LINRIS (Long Intergenic Noncoding RNA for IGF2BP2 Stability) were confirmed by RNA pull-down and RNA immunoprecipitation (RIP) assays. The proliferation and metabolic alteration of CRC cells with LINRIS inhibited were tested in vitro and in vivo. RESULTS: LINRIS was upregulated in CRC tissues from patients with poor overall survival (OS), and LINRIS inhibition led to the impaired CRC cell line growth. Moreover, knockdown of LINRIS resulted in a decreased level of insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2), a newly found N6-methyladenosine (m6A) 'reader'. LINRIS blocked K139 ubiquitination of IGF2BP2, maintaining its stability. This process prevented the degradation of IGF2BP2 through the autophagy-lysosome pathway (ALP). Therefore, knockdown of LINRIS attenuated the downstream effects of IGF2BP2, especially MYC-mediated glycolysis in CRC cells. In addition, the transcription of LINRIS could be inhibited by GATA3 in CRC cells. In vivo experiments showed that the inhibition of LINRIS suppressed the proliferation of tumors in orthotopic models and in patient-derived xenograft (PDX) models. CONCLUSION: LINRIS is an independent prognostic biomarker for CRC. The LINRIS-IGF2BP2-MYC axis promotes the progression of CRC and is a promising therapeutic target.

13.
Sci Rep ; 9(1): 18127, 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31792327

RESUMO

Salidroside (SAL) is the major ingredient of Rhodiola rosea, and has been traditionally used in Chinese medicine for decades. Numerous studies have demonstrated the protective effects of SAL for myocardial ischemia. However, it is yet to be deciphered whether SAL has cardioprotective effects after myocardial infarction (MI) in vivo. In the present study, we established a mouse MI model via coronary artery ligation. The aim was to investigate whether SAL treatment could reduce mortality, improve cardiac function and attenuate myocardial remodeling in MI mice. Post-surgery, mice were randomly administered SAL or normal saline. After 21 days, SAL was found to significantly reduce mortality, improve cardiac function, reduce fibrosis and infarct size compared to normal saline. In addition, oral administration of SAL could attenuate myocardial inflammation and apoptosis and promote angiogenesis. SAL down-regulated the expression levels of TNF-α, TGF-ß1, IL-1ß, Bax and up-regulate the expression of Bcl-2, VEGF, Akt and eNOS. These results indicated that SAL could alleviate the pathological processes of myocardial remodeling in MI mice, and may be a potentially effective therapeutic approach for the management of clinical ischemic cardiovascular diseases.

14.
Biotechnol Bioeng ; 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31803933

RESUMO

Phenylpropionic acids are commonly used in the synthesis of pharmaceuticals, cosmetics, and fine chemicals. However, the synthesis of phenylpropionic acids faces the challenges of high cost of substrates and a limited range of products. Here, we present an artificially designed amino-group-transformation biocatalytic process, which uses simple phenols, pyruvate, and ammonia to synthesize diverse phenylpropionic acids. This biocatalytic cascade comprises an amino-group-introduction module and three amino-group-transformation modules, and operates in a modular assembly manner. Escherichia coli catalysts coexpressing enzymes from different modules achieve whole-cell simultaneous one-pot transformations of phenols into the corresponding phenylpropionic acids including (S)-α-amino acids, α-keto acids, (R)-α-amino acids, and (R)-ß-amino acids. With cofactor recycling, protein engineering, and transformation optimization, four (S)-α-amino acids, four α-keto acids, four (R)-α-amino acids, and four (R)-ß-amino acids are synthesized with good conversion (68%-99%) and high enantioselectivities (> 98%). Therefore, the amino-group-transformation concept provides a universal and efficient tool for synthesizing diverse products. This article is protected by copyright. All rights reserved.

15.
Mol Cell Endocrinol ; 501: 110642, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31759100

RESUMO

The beneficial effects of empagliflozin (EMPA) on cardiac functions during ischemia and reperfusion were characterized. The contractile functions of isolated cardiomyocytes from adult C57BL/6J mice were determined with IonOptix SoftEdgeMyoCam system. The mitochondrial superoxide production was measured by MitoSOX fluorescent probe. The ex vivo isolated heart perfusion system was used to determine the pharmacological effects of EMPA on heart's contractile functions under both physiological and pathological conditions. The in vivo regional myocardial ischemia and reperfusion by ligation of left artery coronary artery descending (LAD) was used to measure the myocardial infarction caused by ischemia and reperfusion with or without EMPA treatment. The results demonstrated that EMPA treatment significantly improves cardiomyocyte contractility under hypoxia conditions and augments the post-ischemic recovery in the ex vivo heart perfusion system. Furthermore, the in vivo myocardial infarction measurement shows that EMPA treatment significantly reduce myocardial infarct size caused by ischemia and reperfusion. The biochemical analysis demonstrated that EMPA can trigger cardiac AMPK signaling pathway and attenuate mitochondrial superoxide production under hypoxia and reoxygenation conditions. In conclusion, EMPA can trigger AMPK signaling pathways and modulate myocardial contractility and reduce myocardial infarct size caused by ischemia and reperfusion independent of hypoglycemic effect. The results for the first time demonstrate that the activation of AMPK by EMPA could one reason about EMPA's beneficial effects on heart disease.

16.
Stem Cell Res ; 41: 101646, 2019 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-31783296

RESUMO

The coronary slow flow phenomenon (CSFP) is characterized by delayed progression of the injected contrast medium through the coronary tree during coronary angiography due to unknown mechanisms. Here, a human induced pluripotent stem cell (iPSC) line (SYSUi002-A) was established using the Sendai-virus delivery system from dermal fibroblasts of a CSFP patient. This cell line may represent a valuable tool for investigating the pathogenesis and therapeutic strategies of CSFP.

17.
Artigo em Inglês | MEDLINE | ID: mdl-31685425

RESUMO

The azido asymmetric stretching motion is widely used for the elucidation of the intrinsic conformational preference and folding mechanism of protein since it has strong vibrational absorbance in the spectral transparent windows. However, the possible secondary structural disturbance induced by the insertion of azido group in the side chain of polypeptides should be carefully evaluated. Here, DFT calculation and enhanced sampling method were employed for model dipeptides with or without azido substitution, and the outcome results show that the lower potential energy basins of isolated model dipeptides are consistent with the preferred structural distributions of model dipeptides in aqueous solution. The azido asymmetric stretching frequency shows its sensitivity to the backbone configurations just like amide-I vibration does, and the azido vibration exhibits great potential as a structural reporter in the transparent window. For the evaluation of the application of azido group in biologically related system, the structural dynamics of Aß37-42 and N3-Aß37-42 fragments and the self-assemble process of their protofiliments in aqueous solution were demonstrated. The outcome results show that the structural fluctuations of Aß37-42 and its protofilament in aqueous solution are quite similar with or without azido substitution, and the dewetting transitions of Aß37-42 and N3-Aß37-42 ß-sheet layers are both complete within 30 ns and assemble into stable protofilaments. Therefore, the azido asymmetric vibrational motion is a minimally invasive structural probe and would not introduce much disturbance to the structural dynamics of polypeptides.

18.
Adv Mater ; : e1904969, 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31736178

RESUMO

Hierarchical porosity and functionalization help to fully make use of metal-organic frameworks (MOFs) for their diverse applications. Herein, a simple strategy is reported to construct hierarchically porous MOFs through a competitive coordination method using tetrafluoroborate (M(BF4 )x , where M is metal site) as both functional sites and etching agents. The resulting MOFs have in situ formed defect-mesopores and functional sites without sacrificing their structure stability. The formation mechanism of the defect-mesopores is elucidated by a combination of experimental and first-principles calculation method, indicating the general feasibility of this new approach. Compared with the original microporous counterparts, the new hierarchical MOFs exhibit superior adsorption for the bulky dye molecules and catalytic performance for the CO2 conversion attributed to their specific hierarchical pore structures.

19.
J Pediatr Surg ; 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31668400

RESUMO

PURPOSE: Lactoferrin has been used as a milk supplement to prevent disease progression in necrotizing enterocolitis. However, the underlying mechanism is still unclear. We hypothesize that lactoferrin administration can modulate intestinal epithelial cell injury. METHODS: We established an in vitro model of epithelial cell injury by treating rat intestinal epithelial cells IEC-18 and human Caco-2 cells with hydrogen peroxide (H2O2), while lactoferrin was added as treatment at the same time. Live/dead cells were detected by immunofluorescence. Gene expression of inflammatory markers interleukin-6 (IL-6), intestinal stem cells (Lgr5), and proliferation marker (Wnt/ß-catenin) were measured. Data was presented as mean ±â€¯SEM and compared using one-way ANOVA. A p-value <0.05 was considered significant. RESULTS: Compared to control cells, H2O2 induced cell death in both IEC-18 and Caco-2 cells, whereas treatment with lactoferrin maintained cell viability. In addition, lactoferrin reduced gene expression of IL-6, while it increased gene expression of Lgr5 and Wnt/ß-catenin. CONCLUSIONS: Intestinal cell injury can be induced by exposure to H2O2, mimicking epithelial damage during intestinal injury. This damage can be reversed by lactoferrin administration by reducing inflammation and inducing cell proliferation. Lactoferrin can be a potential pharmacological intervention for the prevention and recovery of intestinal epithelial injury.

20.
J Pediatr Surg ; 2019 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-31668401

RESUMO

PURPOSE: Cholangitis after Kasai procedure has been previously shown to be related to poor prognosis in Biliary Atresia (BA). To investigate the risk factors and clinical outcomes of cholangitis, we did a retrospective study in post-Kasai BA patients. METHODS: Two-year follow-up data of 180 type-III BA patients after Kasai procedure in 2016 in our hospital were analyzed, including 119 cholangitis patients (66.11%). Among the cholangitis group, patients were further divided into early vs late cholangitis and single vs recurrent cholangitis groups. Liver pathology, liver function, cholangitis occurrence and frequency, jaundice clearance, and survival rates were examined. RESULTS: Higher gamma-glutamyl transferase level before Kasai is a risk factor for cholangitis (p = 0.0393). Older age and higher liver fibrosis score at Kasai are risk factors for recurrent cholangitis (p < 0.05). Shorter prophylactic intravenous antibiotics usage may contribute to early cholangitis, which may lead to higher cholangitis frequency (p < 0.0001). Recurrent cholangitis is associated with earlier cholangitis onsets (p < 0.0001). Cholangitis patients have a relatively delayed jaundice clearance, while early and recurrent cholangitis may contribute to lower overall survival. CONCLUSIONS: Personalized treatment considering risk factors in individual BA patients is needed to prevent cholangitis, especially early onsets, and to improve postoperative outcomes. LEVEL OF EVIDENCE: III.

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