Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 21
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Huan Jing Ke Xue ; 40(1): 318-326, 2019 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-30628289

RESUMO

To improve the efficiency of the removal of dye wastewater, a new type of coagulant "polysilicate ferromanganese (PSFM)" has been synthesized using sodium silicate, ferrous sulfate, and potassium permanganate. Three dyes (direct red, disperse blue, and active yellow) were used for the coagulation tests. The effects of the alkalinity and turbidity on the performance of PSFM were studied. The experimental results show that PSFM performs well with respect to the coagulation of the direct red and disperse blue dyes. The color and TOC removal efficiencies reach 99.2%, 95.4% and 98.5%, and 93.8%, respectively. The coagulation performance is better than that of the conventional coagulants polysilicate iron (PSF), Al2(SO4)3, and FeCl3. The color and TOC removal rates of PSFM for the active yellow dye reach 56% and 51%, respectively. Turbidity has no significant effect on the coagulation efficiency of PSFM. The purification efficiency and alkalinity depend on the amount of dye to be removed. The best alkalinity for the direct red, disperse blue, and active yellow dyes is 50 mg·L-1, 0 mg·L-1, and 75 mg·L-1, respectively. In addition, PSFM has been characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The Zeta potentials of the mixed solutions and flocs during coagulation were also determined. The main indicators of PSFM coagulation are positively charged polynuclear complexes produced by hydrolysis of Fe+ and Mn+ and the bridging polymerization of polysilicon. The adsorption of hydrated manganese dioxide and hydroxyl oxide may also be included.

2.
Huan Jing Ke Xue ; 39(4): 1668-1676, 2018 Apr 08.
Artigo em Chinês | MEDLINE | ID: mdl-29964992

RESUMO

The mechanism of adsorption of a typical antibiotic (tetracycline, TC) on particles in the aquatic environment and the parameters affecting adsorption were investigated. Experiments were conducted to elucidate the effects of pH and various cation species with different concentrations. The results show that the adsorption of TC on particles is rapid during the first four hours in the mixing stage. The adsorption process becomes slow after the first four hours. The adsorption of TC on particles can be described well by a Langmuir equation. The maximum adsorption of TC on particles occurs at pH 4.5, however it is reduced by strongly acidic (pH<4) or alkaline (pH>9) conditions. Moreover, the adsorption process is also inhibited by various cations (e.g. Na+ and Ca2+) in the range of 0.0001-0.1 mol·L-1 ionic concentrations. A special finding concerns Al3+ ions; at a low concentration of these ions (0-0.0001 mol·L-1) the adsorption of TC on particles improves, whereas at increased concentrations the adsorption is weakened. In summary, an effective removal of the particles is critical to control TC pollution in natural waters because of the rapid adsorption of TC on particles.


Assuntos
Antibacterianos/química , Tetraciclina/química , Poluentes Químicos da Água/química , Adsorção , Cátions/química , Concentração de Íons de Hidrogênio
3.
Pest Manag Sci ; 71(3): 404-14, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24777582

RESUMO

BACKGROUND: Strobilurins are one of the most important classes of agricultural fungicides. To discover new strobilurin analogues with broad spectrum and high activity, a series of novel oxime ether strobilurin derivatives containing substituted benzofurans in the side chain were synthesised and bioassayed. RESULTS: The synthesised compounds were characterised by (1) H NMR, (13) C NMR, MS and HRMS. Bioassays demonstrated that most target compounds possessed good or excellent fungicidal activities, especially against Erysiphe graminis and Pyricularia oryzae. Furthermore, methyl 3-methoxypropenoate oxime ethers exhibited remarkably higher activities against E. graminis, Colletotrichum lagenarium and Puccinia sorghi Schw. Notably, (E,E)-methyl 3-methoxy-2-{2-[({[5-fluoro-1-(benzofuran-2-yl)ethylidene]amino}oxy)methyl]phenyl}propenoate (BSF2) and (E,E)-methyl 3-methoxy-2-{2-[({[5-chloro-1-(benzofuran-2-yl)ethylidene]amino}oxy)methyl]phenyl}propenoate (BSF3) were identified as the most promising candidates for further study. CONCLUSION: The present work demonstrates that oxime ether strobilurin derivatives containing benzofurans can be used as possible lead compounds for developing novel fungicides.


Assuntos
Fungos/efeitos dos fármacos , Fungicidas Industriais/química , Fungicidas Industriais/síntese química , Ácidos Graxos Insaturados/síntese química , Ácidos Graxos Insaturados/química , Ácidos Graxos Insaturados/farmacologia , Fungicidas Industriais/farmacologia , Espectroscopia de Ressonância Magnética , Metacrilatos/síntese química , Metacrilatos/química , Metacrilatos/farmacologia , Oximas/síntese química , Oximas/química , Oximas/farmacologia , Relação Estrutura-Atividade
4.
Biochim Biophys Acta ; 1843(11): 2448-60, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25017793

RESUMO

Numerous studies have shown that the NALP3 inflammasome plays an important role in various immune and inflammatory diseases. However, whether the NALP3 inflammasome is involved in the pathogenesis of diabetic nephropathy (DN) is unclear. In our study, we confirmed that high glucose (HG) concentrations induced NALP3 inflammasome activation both in vivo and in vitro. Blocking NALP3 inflammasome activation by NALP3/ASC shRNA and caspase-1 inhibition prevented IL-1ß production and eventually attenuated podocyte and glomerular injury under HG conditions. We also found that thioredoxin (TRX)-interacting protein (TXNIP), which is a pro-oxidative stress and pro-inflammatory factor, activated NALP3 inflammasome by interacting with NALP3 in HG-exposed podocytes. Knocking down TXNIP impeded NALP3 inflammasome activation and alleviated podocyte injury caused by HG. In summary, the NALP3 inflammasome mediates podocyte and glomerular injury in DN, moreover, TXNIP participates in the formation and activation of the NALP3 inflammasome in podocytes during DN, which represents a novel mechanism of podocyte and glomerular injury under diabetic conditions.

5.
Chin Med J (Engl) ; 126(12): 2276-80, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23786938

RESUMO

BACKGROUND: Data on the epidemiology of hypertension in Chinese non-dialysis chronic kidney disease (CKD) patients are limited. The aim of the present study was to investigate the prevalence, awareness, treatment, and control of hypertension in the non-dialysis CKD patients through a nationwide, multicenter study in China. METHODS: The survey was performed in 61 tertiary hospitals in 31 provinces, municipalities, and autonomous regions in China (except Hong Kong, Macao, and Taiwan). Trained physicians collected demographic and clinical data and measured blood pressure (BP) using a standardized protocol. Hypertension was defined as systolic BP ≥ 140 mmHg and/or diastolic BP ≥ 90 mmHg, and/or use of antihypertensive medications. BP < 140/90 mmHg and < 130/80 mmHg were used as the 2 thresholds of hypertension control. In multivariate logistic regression with adjustment for sex and age, we analyzed the association between CKD stages and uncontrolled hypertension in non-dialysis CKD patients. RESULTS: The analysis included 8927 non-dialysis CKD patients. The prevalence, awareness, and treatment of hypertension in non-dialysis CKD patients were 67.3%, 85.8%, and 81.0%, respectively. Of hypertensive CKD patients, 33.1% and 14.1% had controlled BP to < 140/90 mmHg and < 130/80 mmHg, respectively. With successive CKD stages, the prevalence of hypertension in non-dialysis CKD patients increased, but the control of hypertension decreased (P < 0.001). When the threshold of BP < 130/80 mmHg was considered, the risk of uncontrolled hypertension in CKD 2, 3a, 3b, 4, and 5 stages increased 1.3, 1.4, 1.4, 2.5, and 4.0 times compared with CKD 1 stage, respectively (P < 0.05). Using the threshold of < 140/90 mmHg, the risk of uncontrolled hypertension increased in advanced stages (P < 0.05). CONCLUSIONS: The prevalence of hypertension Chinese non-dialysis CKD patients was high, and the hypertension control was suboptimal. With successive CKD stages, the risk of uncontrolled hypertension increased.


Assuntos
Hipertensão/epidemiologia , Insuficiência Renal Crônica/complicações , Adulto , Idoso , Conscientização , Feminino , Humanos , Hipertensão/complicações , Hipertensão/terapia , Masculino , Pessoa de Meia-Idade , Prevalência
6.
Environ Sci Pollut Res Int ; 20(9): 6317-23, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23589259

RESUMO

Transition-metal is known to catalyze peroxymonosulfate (PMS) decomposition to produce sulfate radicals. Here we report reactions between PMS and chloride, without a need of transition metals, also can be used to degrade organic dye pollutant (Rhodamine B, (RhB)). Some important operating parameters, such as dosages of PMS and Cl(-), pH of solution, temperature, ionic strength, and several common cations, were systematically investigated. Almost complete decoloration of RhB was achieved within 5 min ([PMS] = 0.5 mM, [Cl(-)] = 120 mM, and pH 3.0), and RhB bleaching rate increased with the increased dosages of both PMS and chloride ion, following the pseudo-first-order kinetic model. However, the total organic carbon (TOC) removal results demonstrated that the decoloration of RhB was due to the destruction of chromophore rather than complete degradation. RhB decoloration could be significantly accelerated due to the high ionic strength. Increasing of the reaction temperature from 273 K to 333 K was beneficial to the RhB degradation, and the activation energy was determined to be 32.996 kJ/mol. Bleaching rate of RhB with the examined cations increased with the order of NH4 (+) < Na(+) < K(+) < Al(3+) < Ca(2+) < Mg(2+). Some major degradation products of RhB were identified by GC-MS. The present study may have active technical implications for the treatment of dyestuff wastewater in practice.


Assuntos
Cloretos/química , Corantes/química , Oxidantes/química , Peróxidos/química , Rodaminas/química , Concentração de Íons de Hidrogênio , Estrutura Molecular , Concentração Osmolar , Temperatura Ambiente , Fatores de Tempo , Poluentes Químicos da Água/química
7.
PLoS One ; 8(2): e56864, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23437258

RESUMO

OBJECTIVE: Current evidence suggests high serum uric acid may increase the risk of type 2 diabetes, but the association is still uncertain. The aim of the study was to evaluate the association between serum uric acid and future risk of type 2 diabetes by conducting a meta-analysis of prospective cohort studies. DESIGN AND METHODS: We conducted a systematic literature search of the PubMed database through April 2012. Prospective cohort studies were included in meta-analysis that reported the multivariate adjusted relative risks (RRs) and the corresponding 95% confidence intervals (CIs) for the association between serum uric acid and risk of type 2 diabetes. We used both fix-effects and random-effects models to calculate the overall effect estimate. The heterogeneity across studies was tested by both Q statistic and I(2) statistic. Begg's funnel plot and Egger's regression test were used to assess the potential publication bias. RESULTS: We retrieved 7 eligible articles derived from 8 prospective cohort studies, involving a total of 32016 participants and 2930 incident type 2 diabetes. The combined RR of developing type 2 diabetes for the highest category of serum uric acid level compared with the lowest was 1.56(95% CI, 1.39-1.76). Dose-response analysis showed the risk of type 2 diabetes was increased by 6% per 1 mg/dl increment in serum uric acid level (RR 1.06, 95% CI: 1.04-1.07). The result from each subgroup showed a significant association between serum uric acid and risk of type 2 diabetes. In sensitive analysis, the combined RR was consistent every time omitting any one study. Little evidence of heterogeneity and publication bias was observed. CONCLUSIONS: Our meta-analysis of prospective cohort studies provided strong evidence that high level of serum uric acid is independent of other established risk factors, especially metabolic syndrome components, for developing type 2 diabetes in middle-aged and older people.


Assuntos
Diabetes Mellitus Tipo 2/sangue , Ácido Úrico/sangue , Suscetibilidade a Doenças , Humanos , Estudos Prospectivos , Viés de Publicação , Risco
8.
Acta Crystallogr Sect E Struct Rep Online ; 68(Pt 4): m486-7, 2012 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-22589845

RESUMO

In the title compound, [Co(C(10)H(7)N(2)O(2))(2)(H(2)O)(4)], the Co(II) atom lies on an inversion centre and displays a slightly distorted octa-hedral geometry. The coordination sphere is defined by two mutually trans N atoms from two 4-(imidazol-1-yl)benzoate ligands and the O atoms from four water mol-ecules. The crystal structure is stabilized by O-H⋯O hydrogen bonds.

9.
Guang Pu Xue Yu Guang Pu Fen Xi ; 32(11): 3019-23, 2012 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-23387170

RESUMO

Visible infrared spectroscopy (Vis/SW-NIRS) was investigated in the present study for measurement accuracy of soil properties,namely, available nitrogen(N) and available potassium(K). Three types of pretreatments including standard normal variate (SNV), multiplicative scattering correction (MSC) and Savitzky-Golay smoothing+first derivative were adopted to eliminate the system noises and external disturbances. Then partial least squares (PLS) and least squares-support vector machine (LS-SVM) models analysis were implemented for calibration models. Simultaneously, the performance of least squares-support vector machine (LS-SVM) models was compared with three kinds of inputs, including PCA(PCs), latent variables (LVs), and effective wavelengths (EWs). The results indicated that all LS-SVM models outperformed PLS models. The performance of the model was evaluated by the correlation coefficient (r2) and RMSEP. The optimal EWs-LS-SVM models were achieved, and the correlation coefficient (r2) and RMSEP were 0.82 and 17.2 for N and 0.72 and 15.0 for K, respectively. The results indicated that visible and short wave-near infrared spectroscopy (Vis/SW-NIRS)(325-1 075 nm) combined with LS-SVM could be utilized as a precision method for the determination of soil properties.

10.
Cell Calcium ; 50(6): 523-9, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-21959089

RESUMO

Albumin, which is the most abundant component of urine proteins, exerts injurious effects on renal cells in chronic kidney diseases. However, the toxicity of albumin to podocytes is not well elucidated. Here, we show that a high concentration of albumin triggers intracellular calcium ([Ca(2+)](i)) increase through mechanisms involving the intracellular calcium store release and extracellular calcium influx in conditionally immortalized podocytes. The canonical transient receptor potential-6 (TRPC6) channel, which is associated with a subset of familial forms of focal segmental glomerulosclerosis (FSGS) and several acquired proteinuric kidney diseases, was shown to be one of the important Ca(2+) permeable ion channels in podocytes. Therefore we explored the role of TRPC6 on albumin-induced functional and structural changes in podocytes. It was found that albumin-induced increase in [Ca(2+)](i) was blocked by TRPC6 siRNA or SKF-96365, a blocker of TRP cation channels. Long-term albumin exposure caused an up-regulation of TRPC6 expression in podocytes, which was inhibited by TRPC6 siRNA. Additionally, the inhibition of TRPC6 prevented the F-actin cytoskeleton disruption that is induced by albumin overload. Moreover, albumin overload induced expression of the endoplasmic reticulum (ER) stress protein GRP78, led to caspase-12 activation and ultimately podocyte apoptosis, all of which were abolished by the knockdown of TRPC6 using TRPC6 siRNA. These results support the view that albumin overload may induce ER stress and the subsequent apoptosis in podocytes via TRPC6-mediated Ca(2+) entry.


Assuntos
Albuminas/farmacologia , Apoptose , Cálcio/metabolismo , Estresse do Retículo Endoplasmático , Podócitos/metabolismo , Canais de Cátion TRPC/fisiologia , Citoesqueleto de Actina/efeitos dos fármacos , Citoesqueleto de Actina/metabolismo , Animais , Apoptose/efeitos dos fármacos , Sinalização do Cálcio , Caspase 12/metabolismo , Citometria de Fluxo , Camundongos , Microscopia de Fluorescência , Podócitos/citologia , Podócitos/efeitos dos fármacos , RNA Interferente Pequeno , Canais de Cátion TRPC/metabolismo , Transfecção
11.
Acta Crystallogr Sect E Struct Rep Online ; 67(Pt 4): m493, 2011 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-21754003

RESUMO

In the title compound, [Co(C(8)H(5)Cl(2)O(3))(C(12)H(12)N(2))], the Co(II) atom, lying on a twofold rotation axis, is coordinated by four O atoms from two chelating 2,4-dichloro-phen-oxy-acetate ligands and two N atoms from a 5,5'-dimethyl-2,2'-bipyridine ligand, displaying a distorted octa-hedral geometry. A three-dimensional supra-molecular structure is formed through inter-molecular C-H⋯O hydrogen bonds and π-π stacking inter-actions between the pyridine and benzene rings [centroid-centroid distance = 3.779 (2) Å].

12.
Acta Crystallogr Sect E Struct Rep Online ; 67(Pt 5): m606, 2011 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-21754324

RESUMO

In the title compound, [Zn(C(12)H(9)O(2))(2)(C(12)H(12)N(2))]·0.5H(2)O, the water mol-ecule lies on a twofold rotation axis. The Zn(II) atom is coordinated by three O atoms from two 1-naphthyl-acetate ligands, one monodentate and the other asymmetric bidentate chelate, and two N atoms from a 5,5'-dimethyl-2,2'-bipyridine ligand, giving an irregular environment. In the crystal, the complex mol-ecules are inter-linked through the water mol-ecule by O-H⋯O(carboxyl-ate) hydrogen bonds, together with weak C-H⋯O and bipyridine ring π-π stacking inter-actions [ring centroid separation = 3.761 (2) Å], giving a two-dimensional network structure.

14.
Cell Biol Int ; 35(8): 827-34, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21166652

RESUMO

Proteinuria is a well-established exacerbating factor of chronic kidney diseases. However, the harmful effects of protein overload on podocytes and the underlying mechanisms are still poorly understood. In the present study, we examined the effects of high concentrations of albumin on podocytes and investigated the role of CD2AP (CD2-associated protein) in albumin overload-induced podocyte apoptosis. Conditionally immortalized mouse podocytes were cultured in vitro and treated with different concentrations of BSA. In addition, CD2AP eukaryotic expression vector or siRNA (small interfering RNA) was transfected into podocytes before they were exposed to BSA. Podocyte apoptosis, expressions of active caspase-3 (p17) and CD2AP, and the distribution of F-actin cytoskeleton were detected by flow cytometry, Western-blot analysis and fluorescent staining respectively. It was found that exposure of podocytes to BSA induced podocyte apoptosis in a concentration-dependent manner that was accompanied by up-regulation of active caspase-3, the disruption of F-actin cytoskeleton, and decreased expression of CD2AP. Transfection of CD2AP eukaryotic expression vector into podocytes increased CD2AP expression, partially restored F-actin distribution, blocked active caspase-3 expression and inhibited podocyte apoptosis. In contrast, transfection of CD2AP siRNA deteriorated the above changes induced by BSA. It is concluded that protein overload induces podocyte apoptosis via the down-regulation of CD2AP and subsequent disruption of cytoskeleton of podocytes, and CD2AP may play an important role in protein overload-induced podocyte injury.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/biossíntese , Apoptose/efeitos dos fármacos , Proteínas do Citoesqueleto/biossíntese , Podócitos/efeitos dos fármacos , Soroalbumina Bovina/farmacologia , Actinas/análise , Actinas/efeitos dos fármacos , Proteínas Adaptadoras de Transdução de Sinal/genética , Animais , Apoptose/genética , Western Blotting , Caspase 3/biossíntese , Caspase 3/genética , Linhagem Celular , Proteínas do Citoesqueleto/genética , Citoesqueleto/efeitos dos fármacos , Citometria de Fluxo , Rim/metabolismo , Nefropatias , Camundongos , Podócitos/citologia , Podócitos/metabolismo , Proteinúria/metabolismo , Interferência de RNA , RNA Interferente Pequeno , Soroalbumina Bovina/metabolismo , Transdução de Sinais
15.
Cell Biol Int ; 33(6): 632-9, 2009 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-19306938

RESUMO

Emerging evidences show that CD2-associated protein (CD2AP) is involved in podocyte injury and the pathogenesis of proteinuria. However, the exact molecular mechanism by which CD2AP exerts its biological function is elusive. We knocked down CD2AP gene by target siRNA in conditionally immortalized mouse podocytes, which showed lowered cell adhesion and spreading ability (P<0.05). At the same time, cell cycle was arrested in G2/M phase (P<0.05), and pathologic nuclear division could easily be seen in CD2AP siRNA-transfected podocytes. The proliferation of podocytes were also inhibited significantly by CD2AP siRNA transfection (P<0.05). Further study revealed disordered distributions of F-actin, as well as lowered nephrin expression and phosphorylation in podocytes. These data suggest that CD2AP may play a crucial role in maintaining the normal function of podocytes and lowered CD2AP causes podocyte injury by disrupting the cytoskeleton and disturbing the nephrin-CD2AP signaling pathway.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Proteínas do Citoesqueleto/metabolismo , Podócitos/fisiologia , Actinas/metabolismo , Proteínas Adaptadoras de Transdução de Sinal/genética , Animais , Adesão Celular , Linhagem Celular , Movimento Celular , Proliferação de Células , Proteínas do Citoesqueleto/genética , Citoesqueleto , Regulação para Baixo , Fase G2 , Técnicas de Silenciamento de Genes , Proteínas de Membrana/metabolismo , Camundongos , Podócitos/metabolismo , Podócitos/patologia , RNA Interferente Pequeno/metabolismo , Transdução de Sinais , Transfecção
16.
Sheng Li Xue Bao ; 60(1): 135-42, 2008 Feb 25.
Artigo em Chinês | MEDLINE | ID: mdl-18288369

RESUMO

To study the cellular changes and the potential role of CD2-associated protein (CD2AP) in podocyte differentiation, conditionally immortalized murine podocyte cell line was cultured in RPMI 1640 medium under permissive condition at 33 °C. After transfection with CD2AP small interfering RNA (siRNA) the cells were shifted to non-permissive condition at 37 °C. Simultaneously, untransfected cells were taken as differentiation control. The podocyte proliferation rate was determined by MTT method. The expressions of CD2AP, WT1, synaptopodin and nephrin mRNAs were examined by RT-PCR. CD2AP, WT1 and nephrin protein expressions were examined by Western blot. The distribution of CD2AP, nephrin, F-actin and tubulin in differentiated and undifferentiated podocytes was detected by laser scanning confocal microscopy. The results showed: (1) CD2AP, WT1 and nephrin were stably expressed in differentiated and undifferentiated podocytes while synaptopodin was only expressed in differentiated podocytes. (2) CD2AP and nephrin mRNA and protein expressions were up-regulated during podocyte differentiation (P<0.05). (3) CD2AP and tubulin were distributed in the cytoplasm and perinulcear region in undifferentiated podocytes, and F-actin was predominantly localized to a cortical belt and paralleled to the cell axis. Under differentiation condition, CD2AP distribution profile was presented as peripheral accumulation, tubulin took on fascicular style and F-actin extended into foot processes in podocytes. CD2AP colocalized with nephrin and F-actin in undifferentiated podocytes. (4) After transfection with CD2AP siRNA, the expression of CD2AP was partially inhibited and cell growth was arrested; Synaptopodin, the differentiation podocyte marker, was apparently down-regulated; The differentiation of podocytes was delayed. The results demonstrate that podocyte differentiation is accompanied by cytoskeleton rearrangement and cell morphology change. CD2AP might play an essential role in podocyte differentiation.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/fisiologia , Diferenciação Celular , Proteínas do Citoesqueleto/fisiologia , Podócitos/citologia , Actinas/metabolismo , Animais , Linhagem Celular , Citoesqueleto/metabolismo , Proteínas de Membrana/metabolismo , Camundongos , Proteínas dos Microfilamentos/metabolismo , RNA Interferente Pequeno , Transfecção , Proteínas WT1/metabolismo
17.
Zhonghua Yi Xue Za Zhi ; 87(28): 1991-5, 2007 Jul 24.
Artigo em Chinês | MEDLINE | ID: mdl-17923043

RESUMO

OBJECTIVE: To investigate the localization and expression of protein kinase C (PKC)-betaI, betaII in diabetic nephropathy (DN) mouse kidney and its relation to angiotensin receptor blocker telmisartan (Micardis). METHODS: Eighteen mice were divided into three groups: normal group, DN group and Micardis-treated group (n = 6, each group). The expression of PKC-betaI, betaII, transforming growth factor- beta 1 (TGF-beta1) and vascular endothelial growth factor (VEGF) in glomeruli was measured by semiquantitative immunofluorescence histochemistry, the localization of PKC-betaI, betaII was detected by confocal immunofluorescence laser scanning microscopy and the expression of PKC-betaI, betaII in renal cortex, outer and inner medulla were evaluated by semiquantitative Western blotting. RESULTS: Compared to normal mice, the expression of PKC-betaI and betaII on apical membrane of proximal tubule epithelial cells of DN mice was significantly increased, whereas the expression of PKC-betaII on cortical and inner medullary collecting duct was decreased. Western blotting detected increasing expression of PKC-betaI in the renal cortex and outer medulla (P < 0.01), and decreasing expression of PKC-betaII in renal cortex of DN mice (P < 0.01). Enhanced expression of PKC-betaI as well as TGF-beta1 and VEGF (P < 0.01) were shown in the glomeruli of DN mice, where the expression of PKC-betaII was decreased (P < 0.05). Meanwhile, PKC-betaI exhibited a positive correlation to TGF-beta1 (r = 0.649, P = 0.030), but no correlation to VEGF (r = 0.387, P = 0.079). Micardis could partly attenuate above changes. CONCLUSION: The localization and expression of PKC-betaI, betaII are altered in DN mice, PKC-betaI, betaII may change the function of proximal tubule and PKC-betaI may contribute to glomerular hypertrophy through influencing the expression of glomerular TGF-beta1. Treatment with Micardis can partly improve the abnormal expression and distribution of PKC-betaI, betaII in kidneys of DN mice, which suggests that renin-angiotensin-system is implicated in the pathogenesis of DN by regulating the expression and activation of PKC-betaI, betaII isoforms.


Assuntos
Bloqueadores do Receptor Tipo 1 de Angiotensina II/farmacologia , Benzimidazóis/farmacologia , Benzoatos/farmacologia , Nefropatias Diabéticas/prevenção & controle , Rim/efeitos dos fármacos , Proteína Quinase C/metabolismo , Animais , Nefropatias Diabéticas/enzimologia , Nefropatias Diabéticas/patologia , Imunofluorescência , Isoenzimas/metabolismo , Rim/enzimologia , Rim/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Telmisartan , Fator de Crescimento Transformador beta1/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo
18.
Acta Pharmacol Sin ; 28(6): 829-38, 2007 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-17506942

RESUMO

AIM: To investigate the effects of angiotensin receptor blocker (ARB) telmisartan on the expression and distribution of protein kinase C (PKC)-alpha in the kidneys of diabetic mice. METHODS: Diabetic mice were induced with streptozotocin and a group of them were randomly selected for treatment with telmisartan. After 6 weeks, the expression and localization of PKC-alpha in the renal cortex, and the outer and inner medulla were assessed by immunohistochemistry and semiquantitative Western blotting. In addition, expressions of PKC-alpha, transforming growth factor-beta1 (TGF-beta1), and vascular endothelial growth factor (VEGF) in glomeruli were measured by semiquantitative immunohistochemistry. RESULTS: Diabetic and normal mice showed similar distributions of PKC-alpha in the kidneys. The expression of PKC-alpha was found in glomeruli, epithelial cells of proximal tubules, and medullary-collecting duct, while not in the medullary and cortical thick ascending limb, and was different in the epithelial cells of proximal tubules of diabetic nephropathy (DN) mice, PKC-alpha was mostly translocated from the basement membrane to the apical membrane, whereas it was largely translocated from the apical membrane to the basement membrane in epithelial cells of the inner medullary-collecting duct. Western blotting detected increased expression of PKC-alpha in the renal cortex and outer medulla, but not in the inner medulla of DN mice. Enhanced expressions of PKC-alpha, TGF-beta1, and VEGF were shown in the glomeruli of DN mice, where PKC-alpha exhibited a correlation to VEGF, but no correlation to TGF-beta1. ARB telmisartan attenuated alterations of PKC-alpha as mentioned earlier in the DN mice. CONCLUSION: Our findings suggest that PKC-alpha may play a role in the pathogenesis of DN, and that the nephroprotective effects of ARB telmisartan may be partly associated with its influence on PKC-alpha.


Assuntos
Bloqueadores do Receptor Tipo 1 de Angiotensina II/farmacologia , Benzimidazóis/farmacologia , Benzoatos/farmacologia , Diabetes Mellitus Experimental , Rim , Proteína Quinase C-alfa/metabolismo , Animais , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Experimental/patologia , Rim/citologia , Rim/enzimologia , Rim/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Proteína Quinase C-alfa/genética , Distribuição Aleatória , Telmisartan , Fator de Crescimento Transformador beta1/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo
19.
Acta Pharmacol Sin ; 27(5): 579-87, 2006 May.
Artigo em Inglês | MEDLINE | ID: mdl-16626513

RESUMO

AIM: To study the potential pathological role of endogenous angiopoietins in daunorubicin-induced progressive glomerulosclerosis in rats. METHODS: Seventy male Wistar rats were allocated randomly into a daunorubicin group (DRB; n=40) or a control group (n=30). The rats in the DRB group were injected with DRB (15 mg/kg), in their tails. Subsequently, at intervals of 1, 2, 4, 6, 8, and 12 weeks, 5 male Wistar rats in each group were chosen randomly for 24 h urinary protein quantitative measurements (24 h UPQM), and determination of plasma tumor necrosis factor alpha (TNF-alpha), angiopoietin-1 (Ang1), and angiopoietin-2 (Ang2) levels. Kidney sections were examined by electron microscopy, Periodic Acid Schiff (PAS) staining, immunohistochemical staining and in situ hybridization histochemistry. RESULTS: As glomerulosclerosis progressed in the DRB group, expression of Ang1 mRNA and protein in glomeruli decreased and expression of TNF-alpha protein, Ang2 mRNA and protein in glomeruli increased. Expression of Ang1 mRNA and protein in glomeruli were negatively correlated with 24 h UPQM, Fn protein expression, and mean area of extracellular matrix (MAECM). In comparison, expression of Ang2 mRNA and protein in glomeruli were positively correlated with 24 h UPQM, Fn protein expression and MAECM; furthermore, there was a positive correlation between plasma Ang2 and 24 h UPQM. Plasma TNF-alpha and expression of TNF-alpha in glomeruli were positively correlated with expression of Ang2 mRNA and protein in glomeruli. There was a negative correlation between Ang1 protein expression and Ang2 protein expression in glomeruli. CONCLUSION: During DRB-induced glomerulosclerosis, podocyte injury led to a shift in the balance of Ang1 and Ang2 in glomeruli. Increased TNF-alpha in plasma and glomeruli may upregulate Ang2 expression in glomeruli. Elevated Ang2 in both plasma and glomeruli may mediate protein permeability through the glomerular filtration barrier. Moreover, local expression of Ang2 may facilitate the progress of glomerulosclerosis by upregulating a component expression of extracellular matrix.


Assuntos
Angiopoietina-1/biossíntese , Angiopoietina-2/biossíntese , Glomerulosclerose Segmentar e Focal/metabolismo , Glomérulos Renais/metabolismo , Angiopoietina-1/sangue , Angiopoietina-1/genética , Angiopoietina-2/sangue , Angiopoietina-2/genética , Animais , Daunorrubicina , Fibronectinas/sangue , Fibronectinas/metabolismo , Glomerulosclerose Segmentar e Focal/induzido quimicamente , Glomerulosclerose Segmentar e Focal/patologia , Glomérulos Renais/ultraestrutura , Masculino , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Distribuição Aleatória , Ratos , Ratos Wistar , Fator de Necrose Tumoral alfa/metabolismo
20.
Hepatobiliary Pancreat Dis Int ; 5(1): 143-6, 2006 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-16481302

RESUMO

BACKGROUND: The complicated pathogenesis of systemic inflammatory response syndrome (SIRS) is a hot topic in critical care medicine. In this study we explored the expression of toll-like receptor (TLR) 2, 4 of livers in SIRS mice and evaluated the role of TLR2, 4 in the pathogenesis of SIRS. METHODS: Forty BABL/C mice were randomly divided into 2 groups: control (n=20) and SIRS (n=20). SIRS model was induced by severe acute pancreatitis. Blood routine, blood amylase, glutamic pyruvic transaminase and temperature were measured. Histological changes of pancreases and livers were observed microscopically. The mRNA expressions of TLR2, 4 were detected by real-time polymerase chain reaction (PCR). The protein expressions of TLR2, 4 were examined by Western blot. RESULTS: Marked edema, inflammatory cell infiltration, hemorrhage, and necrosis were observed in the pancreases and livers of SIRS mice. The concentrations of amylase and glutamic pyruvic transaminase were increased significantly. Body temperature and white blood cell count were decreased. The mRNA and protein expressions of TLR2, 4 increased markedly in SIRS mice. Significant difference was observed between SIRS and control mice (P<0.01). CONCLUSION: The expressions of TLR2, 4 of livers were increased markedly in SIRS mice, indicating that TLR might play an important role in the pathogenesis of SIRS.


Assuntos
Expressão Gênica , Fígado/metabolismo , RNA Mensageiro/genética , Síndrome de Resposta Inflamatória Sistêmica/metabolismo , Receptor 2 Toll-Like/genética , Receptor 4 Toll-Like/genética , Animais , Modelos Animais de Doenças , Fígado/patologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Reação em Cadeia da Polimerase , RNA Mensageiro/biossíntese , Índice de Gravidade de Doença , Síndrome de Resposta Inflamatória Sistêmica/genética , Síndrome de Resposta Inflamatória Sistêmica/patologia , Receptor 2 Toll-Like/biossíntese , Receptor 4 Toll-Like/biossíntese
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA