Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 157
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Opt Express ; 27(19): 26588-26599, 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31674537

RESUMO

Quantum entanglement and coherence are both essential physical resources in quantum theory. Cold polar molecules have long coherence time and strong dipole-dipole interaction and thus have been suggested as a platform for quantum information processing. In this paper, we employ the pendular states of the polar molecules trapped in static electric fields as the qubits, and put forward several theoretical schemes to generate the entanglement and coherence for two coupled dipoles by using optimal control theory. Through the designs of appropriate laser pulses, the transitions from the ground state to the Bell state and maximally coherent state can be realized with high fidelities 0.9906 and 0.9943 in the two-dipole system, respectively. Meanwhile, we show that the degrees of entanglement and coherence between the two pendular qubits are effectively enhanced with the help of optimized control fields. Furthermore, our schemes are generalized to the preparation of the Hardy state and even to the creation of arbitrary two-qubit states. Our findings can shed some light on the implementation of quantum information tasks with the molecular pendular states.

2.
Nanotechnology ; 30(50): 502003, 2019 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-31491782

RESUMO

Nanomaterials, in addition to their small size, possess unique physicochemical properties that differ from bulk materials, making them ideal for a host of novel applications. Magnetic nanoparticles (MNPs) are one important class of nanomaterials that have been widely studied for their potential applications in nanomedicine. Due to the fact that MNPs can be detected and manipulated by remote magnetic fields, it opens a wide opportunity for them to be used in vivo. Nowadays, MNPs have been used for diverse applications including magnetic biosensing (diagnostics), magnetic imaging, magnetic separation, drug and gene delivery, and hyperthermia therapy, etc. Specifically, we reviewed some emerging techniques in magnetic diagnostics such as magnetoresistive (MR) and micro-Hall (µHall) biosensors, as well as the magnetic particle spectroscopy, magnetic relaxation switching and surface enhanced Raman spectroscopy (SERS)-based bioassays. Recent advances in applying MNPs as contrast agents in magnetic resonance imaging and as tracer materials in magnetic particle imaging are reviewed. In addition, the development of high magnetic moment MNPs with proper surface functionalization has progressed exponentially over the past decade. To this end, different MNP synthesis approaches and surface coating strategies are reviewed and the biocompatibility and toxicity of surface functionalized MNP nanocomposites are also discussed. Herein, we are aiming to provide a comprehensive assessment of the state-of-the-art biological and biomedical applications of MNPs. This review is not only to provide in-depth insights into the different synthesis, biofunctionalization, biosensing, imaging, and therapy methods but also to give an overview of limitations and possibilities of each technology.

3.
Appl Opt ; 58(18): 5090-5097, 2019 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-31503830

RESUMO

Near-infrared spectroscopy (NIRS) is an efficient method for detecting the content of carbon and nitrogen in many materials, which solves the problems of the time-consuming and high-cost traditional chemical analysis method. To quickly detect the carbon-nitrogen ratio (C/N) for the anaerobic fermentation (AF) feedstock using NIRS, a genetic simulated annealing algorithm (GSA) is presented based on a genetic algorithm combined with a simulated annealing algorithm. By combining GSA with backward interval partial least squares (BiPLS), we construct a BiPLS-GSA algorithm to optimize the characteristic wavelength variables of NIRS; this algorithm significantly reduced the number of wavelength variables involved in modeling and effectively improved the detection accuracy and efficiency of the model. The determination coefficients, root mean squared error, mean relative error (MRE) and residual predictive deviation for the validation set in the BiPLS-GSA regression model were 0.9067, 7.6676, 5.5274%, and 3.5626, respectively. Meanwhile, compared to the entire spectrum model, the MRE was decreased by 16.54% in the BiPLS-GSA-based model. The research in this paper improves the adaptability of the prediction model based on optimizing sensitive wavelength variables for C/N, which provides a new way for rapid and accurate measurement of the C/N of AF feedstock.

4.
Pharmacology ; : 1-10, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31390636

RESUMO

BACKGROUND: Experimental and clinical evidence suggests that liver fibrosis is potentially reversible. Hepatic stellate cells (HSCs) play a key role in the development of hepatic fibrosis. Previous clinical applications and researches showed that Siniruangan recipe (SNRG) reversed liver fibrosis and even liver cirrhosis. This experimental study aimed to elucidate the effects of SNRG on the proliferation, apoptosis and activation of HSCs. METHODS: The human HSCs line LX-2 was cultured with normal culture medium and multi-dose SNRG water decoction for 48 h. Cell Counting Kit-8 assay was used to detect the proliferation and cytotoxicity of LX-2 cells. Annexin V-FITC/PI double staining was performed to identify apoptotic cells. Immunofluorescence staining was used to determine the relative content of cleaved caspase-3, tissue inhibitor of metalloproteinase-1 (TIMP-1) and transforming growth factor-ß1 (TGF-ß1) in LX-2 cells. Western blot was used to detect the relative content of Bcl-2, Bax, α-smooth muscle actin, ß-catenin and TIMP-1 protein expression in LX-2 cells. RESULTS: The SNRG inhibited the proliferation of LX-2 and induced cell apoptosis through caspase-dependent and mitochondrial-dependent pathways. SNRG may inhibit the activation of LX-2 through the ß-catenin pathway. The decrease in TIMP-1 and TGF-ß1 protein induced by SNRG promoted the degradation of the extracellular matrix (ECM). CONCLUSIONS: SNRG induced LX-2 cell apoptosis, inhibited cell proliferation, decreased LX-2 cell activity and promoted the degradation of ECM in vitro, which may be important mechanisms for reversing liver fibrosis and liver cirrhosis.

5.
Vet Parasitol ; 272: 31-39, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31395202

RESUMO

The lesswright (lwr) gene and its products are essential molecules in mitosis, DNA repair, and embryo formation in many eukaryotes. In this study, immunohistochemical analysis revealed that the Lwr protein was located in the internal tissues and the surface layer of the adult Schistosoma japonicum (Sj) worms. The mRNA expression levels of SjLwr at different points were evaluated by quantitative real-time RT-PCR. The expression of SjLwr peaked at 14 days and then decreased thereafter. SjLwr expression was relatively more stable in male worms than in female worms. The functions of SjLwr were explored by siRNA-based gene silencing with a simple soaking method. The results showed that knockdown of the SjLwr gene impaired the growth and development of S. japonicum in mice, as well as survival, morphology, reproductive capacity, and egg vitality. These observations imply that SjLwr presents a novel target for the development of immuno- and/or small molecule-based therapeutics for the control and treatment of schistosome infections.


Assuntos
Proteínas de Helminto/metabolismo , Schistosoma japonicum/fisiologia , Esquistossomose Japônica/parasitologia , Animais , Feminino , Regulação da Expressão Gênica , Técnicas de Silenciamento de Genes , Inativação Gênica , Proteínas de Helminto/genética , Masculino , Camundongos , RNA Interferente Pequeno/metabolismo , Reprodução/genética , Schistosoma japonicum/genética , Schistosoma japonicum/crescimento & desenvolvimento , Esquistossomose Japônica/prevenção & controle
6.
Clin Respir J ; 13(9): 545-554, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31295761

RESUMO

OBJECTIVE: This study intended to explore the relation between heart rate recovery at 1 minutes (HRR1) during the recovery phase of cardiopulmonary exercise test (CPET) and exercise capacity in female systemic lupus erythematosus associated pulmonary arterial hypertension (SLE-PAH) patients. METHODS: Twenty-one female SLE-PAH patients underwent right heart catheterization (RHC), pulmonary function test (PFT) and CPET. Forty-two healthy subjects matched with SLE-PAH patients in age, sex and BMI were recruited as a control group. The correlations between HRR1 with clinical and CPET parameters were performed. RESULTS: Peak HR, ΔHR, HRR1, Peak HR-warm HR1min , Peak HR-warm HR2min and CR were significantly lower in SLE-PAH than in controls (P < .01). Increased incidence of CRI was seen in SLE-PAH. Except for the Peak PET O2 , which was higher in controls, all other CPET parameters were lower in SLE-PAH. SLE-PAH patients with HRR1 ≥ 16 had longer 6MWD, lower NT-proBNP, better percent of predicted gas transfer index or diffusing capacity for carbon monoxide (DLco% pred) as well as better CO and CI. Peak HR, ΔHR, HRR1, Peak HR-warm HR1min , Peak HR-warm HR2min , CR, Peak Load, Peak VO2 , Peak PET CO2 , OUEP and OUES were lower and duration of exercise was shorter in patients with HRR1 < 16. HRR1 had positive correlation with 6MWD, DLco% pred, CO, CI and some key CPET parameters. CONCLUSIONS: HRR1 is an easily obtained auxiliary parameter in SLE-PAH patients to reflect an altered autonomic tone. SLE-PAH patients with HRR1 < 16 have more severe hemodynamics, worse clinical findings and marked oxygen uptake inefficiency than those with HRR1 ≥ 16.

7.
Parasit Vectors ; 12(1): 340, 2019 Jul 11.
Artigo em Inglês | MEDLINE | ID: mdl-31296252

RESUMO

BACKGROUND: Schistosoma japonicum (S. japonicum) is an important zoonotic parasite that is prevalent in China and parts of Southeast Asia. Water buffaloes are an important reservoir and the main transmission sources of S. japonicum. However, self-curing and resistance to re-infection have been observed in water buffaloes. RESULTS: In this study, we compared the morphometry and differences in transcriptional expression of adult S. japonicum worms recovered from primary-infected and re-infected water buffaloes using Illumina RNA-sequencing (RNA-Seq) technology. Results of morphometry analysis revealed that adult S. japonicum worms recovered from re-infected water buffaloes were runtish with smaller organs. The ventral length of male worms was shorter in re-infected buffaloes (328 ± 13 vs 273 ± 8 µm, P < 0.05), and in female worms the oral sucker length (44 ± 3 vs 33 ± 5 µm, P < 0.05), ovary length (578 ± 23 vs 297 ± 27 µm, P < 0.05) and width (150 ± 8 vs 104 ± 9 µm, P < 0.05) were shorter, with fewer eggs in the uteri (41 ± 2 vs 12 ± 1, P < 0.05). Of 13,605 identified genes, 112 were differentially expressed, including 51 upregulated and 61 downregulated genes, in worms from re-infected compared with primary-infected water buffaloes. Gene ontology (GO) enrichment analysis revealed that GO terms such as "oxidation-reduction process", "calcium-dependent phospholipid binding", "lipid binding" and "calcium ion binding" were significantly enriched in downregulated genes, whereas GO terms related to metabolism and biosynthesis were significantly enriched in upregulated genes. The results revealed that the downregulation of some important genes might contribute to a reduction in worm numbers and maldevelopment of surviving worms in re-infected water buffaloes. Furthermore, upregulation of genes related to metabolic processes and biosynthesis might be a compensatory mechanism of worms in disadvantageous environments. CONCLUSIONS: To our knowledge, our results present the first large-scale transcriptional expression study identifying the differences between adult S. japonicum worms from primary-infected and re-infected water buffaloes, and particularly emphasize differential expression that may affect the survival and growth of worms in re-infected water buffalo. This will provide new insight into screening for anti-schistosome targets and vaccine candidates.


Assuntos
Búfalos/parasitologia , Reservatórios de Doenças/veterinária , Perfilação da Expressão Gênica , Schistosoma japonicum/genética , Esquistossomose Japônica/veterinária , Animais , China , Feminino , Masculino , Recidiva , Schistosoma japonicum/anatomia & histologia , Esquistossomose Japônica/parasitologia
8.
Clin Respir J ; 13(9): 574-582, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31306554

RESUMO

INTRODUCTION: Venous thromboembolism (VTE) because of lung cancer has been sufficiently studied, nevertheless, little is known regarding the discrepancy of clinical characteristics and predictive factors among different presentations of VTE because of lung cancer. OBJECTIVES: This study was designed to investigate the distinction of clinical characteristics and predictive factors among different presentations of VTE because of lung cancer. METHODS: All patients concomitant lung cancer and VTE were stratified into three groups: pulmonary embolism (PE) group in which patients had sole PE, deep vein thrombosis (DVT) group with sole DVT and concomitance group with both PE and DVT. RESULTS: Concomitance of PE and DVT (28.2 days) mostly occurred at the early stage after the diagnosis of lung cancer, by contrast with DVT (63.6 days) which did at the latest stage, whereas PE (36.7 days) generally developed intermediately in between (P = .02). In a Kaplan-Meier analysis, the cumulative survival rate of DVT group was higher than that of concomitance group, whereas the rate of PE group lied in between. (P = .002) The strongest correlated factors with the development of DVT, PE and concomitance were adenocarcinoma (HR 3.27, P = .003), chemotherapy (HR 2.62, P = .005) and D-Dimer (HR 3.88, P < .001), respectively. The strongest correlated factors with the mortality of DVT, PE and concomitance were comorbidity (HR 2.32, P = .003), metastasis (HR 3.12, P < .001), and metastasis (HR 4.29, P < .001), respectively. CONCLUSION: Concomitance of DVT and PE represents the severest state of lung cancer, the earliest occurrence of VTE, and the worst survival rate, whereas DVT stands for the mildest condition of lung cancer and stablest pattern of VTE.

9.
Acta Parasitol ; 64(3): 638-644, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31172353

RESUMO

INTRODUCTION: Gastrointestinal nematodes (GINs) are a major constraint to the survival and productivity of animals. In southern China, goats are the most important small domestic ruminants. METHODS: From May 2013 to May 2017, we conducted a longitudinal study of hircine GIN infections in Huangshantou Town, Gongan County, Hubei Province, China, using fecal egg counts. RESULTS: Our investigation revealed that the GINs of goats in Hubei Province have changed significantly. Over 90% of eggs detected in the first month of investigation, May 2013, belonged to the species Haemonchus contortus and Chabertia sp. There was no seasonal variation in positive rates (PRs) of GINs, but the mean eggs per gram (EPG) of GINs were higher between April and July than between September and November (P < 0.05). The gradual increase in the percentage of H. contortus eggs among all detected eggs during our research and the low cure rate of IVM mass treatment revealed the emergence of IVM resistance in H. contortus. After the implementation of an integrated GIN control strategy, which included two mass treatments (one in April/May with ABZ and another in September/October with IVM + ABZ), in 2016 and 2017, both the PRs and EPG of GINs were significantly reduced. CONCLUSION: The results presented here reveal that controlling GINs of small ruminants in small farms in southern China requires an integrated control strategy that should include monitoring of infection and anthelmintic resistance, and increased farmer education on the importance of using the appropriate drugs at the correct dose.

10.
Microb Ecol ; 2019 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-31177395

RESUMO

Grasses often establish multiple simultaneous symbiotic associations with endophytic fungi and arbuscular mycorrhizal fungi (AMF). Many studies have examined pair-wise interactions between plants and endophytic fungi or between plants and AMF, overlooking the interplays among multiple endosymbionts and their combined impacts on hosts. Here, we examined both the way in which each symbiont affects the other symbionts and the tripartite interactions between leaf endophytic fungi, AMF, and Leymus chinensis. As for AMF, different species (Glomus etunicatum, GE; Glomus mosseae, GM; Glomus claroideum, GC; and Glomus intraradices, GI) and AMF richness (no AMF, single AMF taxa, double AMF mixtures, triple AMF mixtures, and all four together) were considered. Our results showed that significant interactions were observed between endophytes and AMF, with endophytes interacting antagonistically with GM but synergistically with GI. No definitive interactions were observed between the endophytes and GE or GC. Additionally, the concentration of endophytes in the leaf sheath was positively correlated with the concentration of AMF in the roots under low AMF richness. The shoot biomass of L. chinensis was positively related to both endophyte concentration and AMF concentration, with only endophytes contributing to shoot biomass more than AMF. Endophytes and AMF increased shoot growth by contributing to phosphorus uptake. The interactive effects of endophytes and AMF on host growth were affected by the identity of AMF species. The beneficial effect of the endophytes decreased in response to GM but increased in response to GI. However, no influences were observed with other GC and GE. In addition, endophyte presence can alter the response of host plants to AMF richness. When leaf endophytes were absent, shoot biomass increased with higher AMF richness, only the influence of AMF species identity outweighed that of AMF richness. However, when leaf endophytes were present, no significant association was observed between AMF richness and shoot biomass. AMF species identity rather than AMF richness promoted shoot growth. The results of this study demonstrate that the outcomes of interspecific symbiotic interactions are very complex and vary with partner identity such that the effects of simultaneous symbioses cannot be generalized and highlight the need for studies to evaluate fitness response of all three species, as the interactive effects may not be the same for each partner.

11.
ACS Appl Mater Interfaces ; 11(26): 22979-22986, 2019 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-31252472

RESUMO

Magnetic nanoparticles (MNPs) have been extensively used as contrasts and tracers for bioimaging, heating sources for tumor therapy, carriers for controlled drug delivery, and labels for magnetic immunoassays. Here, we describe a MNP Brownian relaxation dynamics-based magnetic particle spectroscopy (MPS) method for the quantitative detection of molecular biomarkers. In MPS measurements, the harmonics of oscillating MNPs are recorded and used as a metric for the freedom of rotational motion, which indicates the bound states of the MNPs. These harmonics can be collected from microgram quantities of iron oxide nanoparticles within 10 s. As the harmonics are largely dependent on the quantity of the MNPs in the sample, the MPS bioassay results could be biased by the deviations of MNP quantities in each sample, especially for the very low-concentration biomarker detection scenarios. Herein, we report three MNP concentration/quantity-independent metrics for characterizing the bound states of MNPs in MPS. Using a streptavidin-biotin binding system as a model, we demonstrate the feasibility of using MPS and MNP concentration/quantity-independent metrics to sense these molecular interactions, showing that this method can achieve rapid, wash-free bioassays, and is suitable for future point-of-care, sensitive, and versatile diagnosis.

12.
Parasit Vectors ; 12(1): 196, 2019 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-31046821

RESUMO

BACKGROUND: Yellow cattle and water buffalo are important natural reservoir hosts and the main transmission sources of Schistosoma japonicum in endemic areas of China. The worms from the two hosts have marked differences in general worm morphology and ultrastructure, gene transcription and protein expression profiles. RESULTS: To investigate microRNAs (miRNAs) involved in the regulation of schistosome development and survival, we compared miRNA expression profiles of adult schistosomes derived from yellow cattle and water buffalo by using high-throughput sequencing with Illumina Hiseq Xten. Schistosoma japonicum from water buffalo and yellow cattle yielded 63.78 million and 63.21 million reads, respectively, of which nearly 50% and 49% could be mapped to selected miRNAs in miRbase. A total of 206 miRNAs were identified, namely 79 previously annotated miRNAs of S. japonicum and 127 miRNAs that matched with the S. japonicum genome and were highly similar to the annotated miRNAs from other organisms. Among the 79 miRNAs, five (sja-miR-124-3p, sja-miR-219-5p, sja-miR-2e-3p, sja-miR-7-3p and sja-miR-3490) were significantly upregulated in the schistosomes from water buffalo compared with those from yellow cattle. A total of 268 potential target genes were predicted for these five differentially expressed miRNAs. Eleven differentially expressed targets were confirmed by qRT-PCR among 15 tested targets, one of which was further validated through dual-luciferase reporter assay. Among the 127 'possible' S. japonicum miRNAs, ten were significantly differentially expressed in the schistosomes from these two hosts. CONCLUSIONS: These results highlight the important roles of miRNAs in regulating the development and survival of schistosomes in water buffalo and yellow cattle and facilitate understanding of the miRNA regulatory mechanisms in schistosomes derived from different susceptible hosts.


Assuntos
Búfalos/parasitologia , Doenças dos Bovinos/parasitologia , MicroRNAs/genética , RNA de Helmintos/genética , Schistosoma japonicum/genética , Schistosoma japonicum/isolamento & purificação , Esquistossomose Japônica/parasitologia , Animais , Bovinos , Feminino , Perfilação da Expressão Gênica , Masculino , MicroRNAs/metabolismo , RNA de Helmintos/metabolismo , Schistosoma japonicum/classificação , Schistosoma japonicum/crescimento & desenvolvimento
13.
Waste Manag ; 89: 303-312, 2019 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-31079744

RESUMO

Energy utilization efficiency of heating for the operation process of biogas reactor is an important factor limiting its development and popularization. A novel mode of solar radiant heating combined with the conventional heating mode was proposed to reduce the power loss and improve the utilization cycle of heat exchanger. In present work, experimental and numerical researches about the anaerobic fermentation process under two heating modes were made to investigate the effect of temperature fluctuation on non-isothermal fermentation process under solar radiant heating. The results show that the methane production capacity of non-isothermal process under solar radiant heating reduces by up to 14% compared with the constant temperature condition in three seasons; increasing the total solid concentration of bioreactor is helpful for improving the effect of solar radiant heating; the effects of temperature fluctuation coefficient on acid and methane productions are bigger than the one on pH of slurry.


Assuntos
Reatores Biológicos , Calefação , Biocombustíveis , Metano , Luz Solar , Temperatura Ambiente
14.
Clin Respir J ; 13(3): 148-158, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30667180

RESUMO

BACKGROUND: Pulmonary hypertension patients with mean pulmonary artery pressure (mPAP) ≥ 25 mm Hg had impaired cardiopulmonary exercise testing (CPET). Borderline mean pulmonary pressures (boPAP; 21-24 mm Hg) represent early pulmonary vasculopathy. The CPET characteristics of boPAP are a matter of discussion. We aimed to determine the CPET profile of such borderline hemodynamics. METHODS: A matched case-control study was conducted on consecutive boPAP patients at the Shanghai Pulmonary Hospital between Jan 2012 and Jan 2017. Hemodynamics, echocardiography, the pulmonary function test (PFT) and CPET parameters were compared between boPAP patients and normal mPAP patients which were matched 1:1 by sex and age. Conditional logistic regression analysis was performed to determine the efficacy of CPET in detecting boPAP. RESULTS: A total of 48 patients underwent RHC and CPET (24 Normal, 24 boPAP). There were no differences in the demographics, echocardiography and PFT. BoPAP patients had significantly decreased VO2 at the anaerobic threshold and peak VO2 /kg (858.4 ± 246.5 mL/min vs 727.9 ± 228.0 mL/min, P = 0.037; 21.1 ± 6.4 mL/min/kg vs. 15.5 ± 5.6 mL/min/kg, P = 0.001, respectively). Significant differences were not observed in ventilation efficiency. A trend of impaired oxygen pulse and submaximal exercise tolerance were observed in boPAP patients. Conditional logistical regression analysis revealed the risk of boPAP increased by 2.493 (95% confident interval: 1.388 to 4.476, P = 0.002) with every 5 mL/min/kg decrease in peak VO2 /kg. CONCLUSIONS: Patients with boPAP have a greater prevalence of exercise intolerance, a trend of impaired oxygen pulse and submaximal exercise tolerance.


Assuntos
Teste de Esforço/métodos , Hipertensão Pulmonar/diagnóstico , Artéria Pulmonar/fisiopatologia , Adulto , Idoso , Estudos de Casos e Controles , China , Ecocardiografia , Feminino , Hemodinâmica , Humanos , Hipertensão Pulmonar/fisiopatologia , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Testes de Função Respiratória
15.
BMC Pulm Med ; 18(1): 161, 2018 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-30326867

RESUMO

BACKGROUND: Risk assessment of pulmonary arterial hypertension (PAH) contributes to its management. Unfortunately, the existing risk assessment approaches are defective for clinicians to practice in daily clinical settings to some extent. METHODS: We designed a modified Risk Assessment Score of PAH (mRASP) comprising four non-invasive variables which were World Health Organization functional class(WHO FC), 6-min walk distance (6MWD), N-terminal of the pro-hormone brain natriuretic peptide(NT-pro BNP), and right atrial area(RAA), then validated it in the prediction of one-year survival rate for patients with PAH by contrast with the REVEAL risk score. RESULTS: For the validation cohort(n = 216), the predicted one-year survival rate were 95-100%, 90-95%, and < 90% in the mRASP risk score strata of 0-2, 3-5, and 6-8, respectively; meanwhile, the observed one-year survival rates were 97.1, 92.6, and 52.2%, in each corresponding stratum, respectively. The mRASP (c-index = 0.727) demonstrated similar predictive power in contrast with the REVEAL risk assessment score (c-index = 0.715) in the prediction of one-year survival rate. CONCLUSION: The mRASP is an eligible risk assessment tool for the prognostic assessment of PAH. In contrast with the REVEAL score, it demonstrated similar predictive power and accuracy, with extra simplicity and convenience.

16.
Vet Parasitol ; 261: 18-21, 2018 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-30253847

RESUMO

Schistosomiasis is a zoonotic parasitic disease threatening tens of millions people and farm animals. Water buffalos are a major reservoir for schistosomiasis and a control target. Epidemiological surveys suggest that buffalos can develop resistance against Schistosoma japonicum reinfection. In the present paper, relative to control animals, we report an over 97% worm burden reduction after two rounds of infection with S. japonicum and treatment with Praziquantel (PZQ). Relative to control animals, shorter length of female worms, and lower egg counts (over 87.7% reduction rates) were observed in reinfected buffalos. We also found that the reinfected buffalos had significantly higher levels of IL-4, IL-10, and IFN-γ, 4-9 weeks after the secondary infection, and a significantly higher level of specific IgG antibodies before infection. Our results confirmed that after infection buffalos develop resistance against S. japonicum reinfection, and that this resistance is mainly due to acquired immunity. These findings may aid in the future vaccine design for water buffalos.


Assuntos
Imunidade Adaptativa , Búfalos , Resistência à Doença/imunologia , Schistosoma japonicum/fisiologia , Esquistossomose Japônica/veterinária , Animais , Masculino , Esquistossomose Japônica/imunologia , Esquistossomose Japônica/parasitologia
17.
3 Biotech ; 8(9): 403, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30221116

RESUMO

Fatty acids are the precursors for the production of fuels, oleochemicals and special health care products. In this study, a novel rapid method for fatty acid (FA) preparation by the enzymatic hydrolysis of Phoenix tree seed, an undeveloped woody oil seed, was developed. High-temperature GC with flame ionization detector (FID) and the hydrolysis ratio were used to monitor reaction progress. Enzyme screening and the effect of reaction variables on the hydrolysis of seeds were evaluated and optimized by response surface methodology. The results showed that among the tested enzymes, Lipozyme TLIM showed the greatest amount of hydrolysis of Phoenix tree seed. FAs can be rapidly prepared by one-step hydrolysis of Phoenix tree seeds using Lipozyme TLIM as the biocatalyst. Under the optimized conditions (6% enzyme load, 1:8 mass ratio of seed to water, 47.7 °C and 16 min), the maximum hydrolysis ratio (96.4 ± 1.1%) can be achieved. The effect of reaction variables on the hydrolysis decreased in the following order: reaction time > enzyme load > substrate ratio of seed to water > reaction temperature. This work provides a novel and rapid method for FA preparation from oil seeds.

18.
BMC Pulm Med ; 18(1): 143, 2018 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-30139350

RESUMO

BACKGROUND: Anemia is prevalent not only in COPD but also in pulmonary hypertension. We postulated that anemia may have certain prognostic value in COPD concomitant with PH due to COPD (COPD-PH). METHODS: We performed a 12-month prospective investigation to follow up COPD patients with or without PH assessed by right heart catheterization. Eligible patients were enrolled, stratified into COPD-PH-anemia group (n = 40), COPD-PH group (n = 42), COPD-anemia group (n = 48), and COPD group(n = 50), and then followed up for 12 months. RESULTS: After the follow-up, for both of the actual variation value and variation rate, the increase of NT-pro BNP (P<0.001; P = 0.03) and CAT score (P = 0.001; 0.002), as well as the decrease of PaO2 (P = 0.03; 0.086) and Peak VO2 (P = 0.021; 0.009) in COPD-PH-anemia group were highest among four groups. The cumulative one-year survival rates were similar among four groups (P = 0.434). The cumulative exacerbation-free rate was lowest in COPD-PH-anemia group among four groups (P<0.001). Hemoglobin was an independent promoting factor for the probability of hospitalization due to exacerbation ≧ 1/year in patients with COPD-PH-anemia [HR 3.121(2.325-5.981); P<0.001]. CONCLUSIONS: Anemia is a promoting factor for the worsening of exercise capacity, deterioration of hypoxemia, declining of life quality, and aggravation of exacerbations in patients with COPD-PH-anemia, by contrast with COPD-PH, COPD-anemia, and COPD.

20.
Opt Express ; 26(16): 20122-20131, 2018 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-30119327

RESUMO

The artificial field can be generated by properly arranging pulsed magnetic fields interacting with a Bose-Einstein condensate (BEC), which can be widely used to simulate the phenomena of traditional condensed matter physics, such as spin-orbit (SO) coupling and the neutral atom spin Hall effect. The introduction of SO coupling in a BEC will alter its optical properties. Eletromagnetically induced transparency (EIT) is a powerful tool that can change and detect the properties of an atomic medium in a nondestructive way. It is important and interesting to study EIT properties and to investigate the effects of SO coupling on EIT. In this paper, we investigate EIT in a SO-coupled BEC. Not only is the transparency existing, but the real and imaginary parts of the susceptibility have an additional red frequency shift, which is linearly proportional to the strength of the SO coupling. By using this unconventional, sensitive EIT spectrum, SO coupling can be detected and its strength can be accurately measured according to the frequency shift.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA