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1.
Food Chem ; 371: 131206, 2022 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-34619635

RESUMO

A DIA-based quantitative proteomic strategy was used to investigate the effects of different cooking procedures (steaming and boiling) on pork meat quality. Results showed that steamed meats had higher redness, cohesion, springiness, but lower lightness, yellowness, shear force, hardness, chewiness and cooking loss than boiled meats. In total of 1608 proteins were identified and 103 proteins exhibited significant difference (fold change > 1.5, P < 0.05). These DAPs mainly involved in protein structure, metabolic enzyme, protein turnover and oxidation stress. ALDOC, PVALB, PPP1R14C, AMPD1, CRYAB and SOD1 were validated as potential indicators of color variations in cooked meat. CFL1, COL1A1, COL3A1, RTN4, NRAP, NT5C3A, and SOD1 might be potential biomarker for texture changes of cooked meats. Moreover, these validated proteins exhibited significant (P < 0.05) correlation with cooking loss and could be serve as candidate predictors for cooking loss changes of meats in different cooking procedures.


Assuntos
Carne de Porco , Proteômica , Animais , Culinária , Dureza , Carne/análise , Suínos
2.
Food Chem ; : 131614, 2021 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-34848091

RESUMO

As the major byproduct of meat processing, bovine bone are produced in large amounts annually. However, the inefficient utilization with low-added value resulted in serious resource waste. The study aims to prepare high-value bovine bone power (BBP) via instant catapult steam-explosion (ICSE) treatment, taking ball milling (BM) method as control. Results showed that ICSE treatment deconstructed bovine bone with more holes emerging, and effectively promoted mineral dissolution and protein degradation while reduced energy consumption. Compared with BM-BBP, ICSE-BBP possessed more protein and essential minerals, presenting in regular elliptical shapes with narrow distribution of particle size (0.1 âˆ¼ 40 µm), and owned better solution stability and protein solubility. ICSE-BBP also exhibited higher mineral release and protein digestibility during GI digestion while revealed no obvious cytotoxicity, indicating the potential applicability in nutrition-fortified foods. Taken together, ICSE technology holds promise in reusing bovine bone, providing an efficient and eco-friendly process for BBP industrial production.

3.
Food Res Int ; 145: 110401, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-34112404

RESUMO

As a kind of promising resource, animal bone has been widely processed into functional foods. However, there is little research about the effect of particle size on the physicochemical properties and digestibility of yak bone powder (YBP), as well as its anti-osteoporosis activity. In this study, the YBP with median particle sizes (MPS) ranging from 19.68 to 128.37 µm were prepared, and their digestibility and anti-osteoporosis activity were investigated. The results showed that smaller MPS significantly increased water holding capacity and protein solubility without changing composition. The MPS reduction greatly promoted protein digestion, producing more peptides<3 kDa and free amino acids while decreased Ca2+ and P5+ release during gastrointestinal digestion. The in vivo results revealed the positive effect of YBP on ovariectomy-induced osteoporosis in rats. The bone mineral density of ovariectomized (OVX) rats was obviously improved by regulating bone turnover markers (B-ALP, OCN, S-CTX, ES and TRAP), thus potentially shedding light on osteoporosis remission. However, different MPS exhibited a weak effect on osteoporosis in OVX rats. Therefore, YBP could be produced in relatively large particle size without sacrificing food sensory quality, the processing time of which could also be shortened for higher productivity and lower cost.


Assuntos
Osteoporose , Animais , Osso e Ossos , Bovinos , Modelos Animais de Doenças , Feminino , Humanos , Ovariectomia , Tamanho da Partícula , Pós , Ratos
4.
Food Chem ; 357: 129765, 2021 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-33957473

RESUMO

The oxidation and volatile constituents of bacon (which made of white pig (Duroc × Landrace × Yorkshire) and black pig (Beijing Black × Yorkshire)) during refrigerated storage were analyzed by thiobarbituric acid reactive substances (TBARS), total thiol groups, gas chromatography-mass spectrometry (GC-MS) and gas chromatography coupling with ion mobility spectrometry (GC-IMS). The TBARS value of tested samples increased while the total thiol groups decreased during 60 days storage (P < 0.05). Volatile fingerprint results were obtained by GC-MS and GC-IMS detector, the classes and contents of volatile compounds detected in WP bacon were much abundant than BP bacon. Higher phenols and acids concentrations were observed in WP bacon, while alcohols, ketones, and nitrogen-containing compounds were more determined in BP bacon. Besides, WP bacon was more notably affected by storage rather than BP bacon, and the difference of raw material is the main reason for the flavor in WP and BP bacon comparing with the storage process.

5.
Food Res Int ; 129: 108832, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-32036888

RESUMO

The main purpose of this research was to evaluate the element distribution in the edible viscera of Tibetan pigs and to clarify its correlation with soils, drinking water and feed. A total of 55 chemical elements were simultaneously quantified. P, K, Na, Mg, Ca, Fe and Zn were the most abundant elements in the analyzed viscera. The general distribution of all element concentrations in the viscera of Tibetan pigs was such that liver > kidney > small intestine > heart = lung = large intestine > stomach. Comparison with national and international allowable limits of toxic elements indicates that consumption of Tibetan pig viscera presents potential health risks. Spearman correlation analysis reveals that significantly (p < 0.0001) positive relationships exist between the element profiles of viscera and drinking water, soils as well as feed. For all edible viscera, the largest values of correlation coefficient were observed to be with corn feed. Our research provides a relatively comprehensive investigation of the elemental composition in Tibetan pig viscera. The correlation data would be helpful for the local farm to reformulate the feed for Tibetan pigs to improve the quality and safety of the viscera.


Assuntos
Ração Animal , Meio Ambiente , Vísceras/química , Animais , Água Potável , Intestino Delgado , Rim , Fígado , Solo , Estômago , Suínos , Tibet , Zea mays
6.
Sci Rep ; 9(1): 9993, 2019 07 10.
Artigo em Inglês | MEDLINE | ID: mdl-31292502

RESUMO

Acute infectious gastroenteritis cases in Shanghai, reported over three years, were analyzed. Pathogens were identified in 1031 patients; of these, 725 and 306 were bacterial and viral cases, respectively. Vibrio parahemolyticus and Salmonella were the dominant bacteria, and Caliciviridae and Reoviridae were the dominant viral families in the local area. The acute gastroenteritis epidemic peaks appeared in August and January, which represented the active peak periods of bacteria and viruses, respectively. Logistic regression analyses with sex stratification showed that abdominal pain, fever and ingestion of unsafe food at restaurants were independent factors more frequently associated with bacterial gastroenteritis irrespective of sex; red cell-positive fecal matter was associated with bacterial gastroenteritis with an odds ratio (OR) of 3.28 only in males; and white blood cell count was associated with bacterial gastroenteritis with an OR of 1.02 only in females. Pathogen stratification showed that age, vomiting and red cell-positive fecal matter were associated with males with ORs of 0.99, 0.61 and 1.71, respectively, in bacterial gastroenteritis; and the migrant ratio was higher in males with an OR of 2.29 only in viral gastroenteritis. In conclusion, although bacterial and viral gastroenteritis shared many features, epidemiological and clinical factors differed between sexes and pathogens.


Assuntos
Caliciviridae/isolamento & purificação , Doenças Transmissíveis/epidemiologia , Gastroenterite/microbiologia , Reoviridae/isolamento & purificação , Salmonella/isolamento & purificação , Vibrio parahaemolyticus/isolamento & purificação , Adulto , China/epidemiologia , Feminino , Gastroenterite/epidemiologia , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Vigilância da População , Fatores de Risco , Estações do Ano , Caracteres Sexuais
7.
Food Res Int ; 119: 426-435, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30884673

RESUMO

The primary aim of this study was to unravel key proteins for the differentiation of Tibetan (n = 15) and Duroc × (Landrace × Yorkshire) (n = 15) pork. A platform consisting of LC-MS/MS analysis and label-free quantitative proteomics was utilized. Changes in the proteome profiles were observed for different pork cuts. A total of 91 and 116 differentially expressed proteins (fold change >2 or < 0.5, p-value<.05) were identified in the five cuts (e.g., shoulder, rump, loin, shank and belly) of Tibetan (TP) and Duroc × (Landrace × Yorkshire) (DLY) pork, respectively. Meanwhile, a comparative proteomics analysis was performed between the TP and DLY pork. We identified 102 expression altered proteins, of which 52.9% (n = 54) and 47.1% (n = 48) were up- and downregulated, respectively, in DLY pork compared to TP. Functional analysis of these proteins revealed that the most significantly enriched gene ontology term for the biological process was the purine-containing compound metabolic process (p = .003), while that with respect to molecular function was threonine-type peptidase activity (p = .002) and that for the cellular component was the mitochondrial inner membrane (p = .001). The most significantly enriched KEGG pathway was involved in histidine metabolism (p = .01), followed by oxidative phosphorylation (p = .02). Proteins involved in oxidative phosphorylation were overabundant in TP. Using a chemometrics approach, we identified 68 significant proteins for the discrimination of TP and DLY pork. The most significantly upregulated proteins in TP and DLY pork were nicotinamide nucleotide transhydrogenase and heat shock protein 90-beta, respectively. This study demonstrates the feasibility of using differential proteomic analysis to discriminate between TP and DLY pork, and the current dataset can be expanded to a larger sample size for possible discriminant validation.


Assuntos
Proteínas/análise , Proteômica , Carne Vermelha/análise , Animais , Cromatografia Líquida , Sus scrofa , Suínos , Espectrometria de Massas em Tandem , Tibet
8.
Oncotarget ; 8(4): 6079-6084, 2017 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-28030828

RESUMO

Methicillin-resistant Staphylococcus aureus (MRSA) strains are now common both in the health care setting and in the community. Active surveillance is critical for MRSA control and prevention. Specimens of patients (200 patients with 1119 specimens) as well as medical staff and hospital setting (1000 specimens) were randomly sampled in a level 2 hospital in Shanghai from September 2011 to August 2012. Isolation, cultivation and identification of S. aureus were performed. Totally, 67 S. aureus strains were isolated. 32 S. aureus strains were isolated from patient samples; 13 (13/32, 40.6%) of the 32 S. aureus isolates were MRSA; sputum sample and patients in the department of general internal medicine were the most frequent specimen and patient group for S. aureus strains isolation. Remaining 35 S. aureus strains were isolated from the medical staff and hospital setting; 20 (20/35, 57.1%) of the 35 S. aureus isolates were MRSA; specimens sampled from doctors and nurses' hands and nose and hospital facilities were the most frequent samples to isolate S. aureus. Resistant and virulent genes detection showed that, all 33 MRSA strains were mecA positive which accounts for 49.3% of the 67 S. aureus strains; 38 isolates were Panton-Valentine leukocidin (PVL) gene positive which accounts for 56.7% of the 67 S. aureus strains; and 17 (17/67, 25.4%) isolates are mecA and PVL genes dual positive. Multidrug-resistant strains of MRSA and PVL positive S. aureus are common in patients, medical staff and hospital setting, the potential health threat is worthy of our attention.


Assuntos
Proteínas de Bactérias/genética , Toxinas Bacterianas/genética , Exotoxinas/genética , Leucocidinas/genética , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Proteínas de Ligação às Penicilinas/genética , Infecções Estafilocócicas/diagnóstico , Mãos/microbiologia , Humanos , Staphylococcus aureus Resistente à Meticilina/classificação , Staphylococcus aureus Resistente à Meticilina/genética , Nariz/microbiologia , Vigilância da População , Escarro/microbiologia
9.
Artigo em Chinês | MEDLINE | ID: mdl-24645332

RESUMO

OBJECTIVE: Traditional detection approaches for non-O157 STEC are both time and labour consuming in diseases surveillance. Virulence genes detection based on multiplex PCR could not only improve the detection efficiency but also increase the accuracy. METHODS: Six virulence genes of non-O157:H7 (stx1, stx2, eae, hly, etpD, katP6) were detected by two groups of trebling PCRs. The multiplex PCRs were optimized by melting curve analysis in SYBR Green I real-time PCR. Testing result of multiplex PCR was consistent with serological testing. RESULTS: The sensitivity limits of the multiplex PCR for stx1, stx2, eaeP, etpD, katP, and hly were 10 ng/ml, 120 ng/ml, 110 ng/ml,165 ng/ml, 85 ng/ml, and 15 ng/ml, respectively, which is similar with that of single PCR. When the multiplex PCR was applied in 120 adults and 90 children diarrhea samples detection, 13 cases were detected for non-O157 positive. CONCLUSION: The method we established can be used for non-O157 STEC virulence genes detection and screening with high efficiency and accuracy.


Assuntos
Infecções por Escherichia coli/microbiologia , Reação em Cadeia da Polimerase Multiplex/métodos , Escherichia coli Shiga Toxigênica/isolamento & purificação , Fatores de Virulência/genética , Infecções por Escherichia coli/diagnóstico , Proteínas de Escherichia coli/genética , Humanos , Escherichia coli Shiga Toxigênica/genética
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