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1.
J Hazard Mater ; 401: 123311, 2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-32652417

RESUMO

Thallium (Tl), is a highly toxic trace metal in the natural environment. Emerging Tl pollution in waters has gradually become a global concern. However, limited removal technologies are available for Tl-containing wastewater. Herein, MnFe2O4-biochar composite (MFBC) was successfully fabricated via coprecipitation method as a novel and efficient adsorbent for treating Tl(I)-contaminated wastewater. It was found that the MFBC, with a specific surface area of 187.03 m2/g, exhibited high performance across a wide pH range of 4-11, with the superior Tl(I) removal capacity (170.55 mg/g) based on Langmuir model (pH 6.0, a dosage of 1 g/L). The removal mechanisms included physical and chemical adsorption, ion exchange, surface complexation, and oxidation. This investigation revealed that MFBC is a promising and environmentally friendly adsorbent with a low cost, large specific surface area, magnetic properties, and high efficiency for the removal of Tl(I) from wastewater.

2.
J Nanosci Nanotechnol ; 21(2): 1148-1153, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33183455

RESUMO

In order to investigate the role of composite nanoparticles in the immune stage, we observed the expression of R-1 in anti-mycotic infection of human corneal epithelial cells, and divided the experiment into control group, fungal stimulation group, Gw507 blocking group and Dectin-1. In the inhibitor group, the immune effect of human corneal epithelial cells was studied. Subsequently, the expression of total R-1 and phosphorylated form of p-R-1 in each experimental group was detected by western blot method. Finally, real-time quantitative PCR was used to detect the expression of factors IL-6 and IL-8. We concluded that p-R-1 was slightly expressed in the control group. After 15 minutes of fungal stimulation, the expression level in the experimental group was significantly higher than that in the blank group, and the difference was statistically significant (p < 0.05). In the blocking group of two different inhibitors, the expression of p-R-1 was lower than that in the fungal stimulation experimental group, and the difference was statistically significant (p < 0.05). Therefore, R-1 is expressed in corneal epithelial cells and the fungus exerts its antifungal effect through the Dectin-1 signaling pathway.

3.
Nat Commun ; 11(1): 5653, 2020 11 06.
Artigo em Inglês | MEDLINE | ID: mdl-33159076

RESUMO

Scars are more severe when the subcutaneous fascia beneath the dermis is injured upon surgical or traumatic wounding. Here, we present a detailed analysis of fascia cell mobilisation by using deep tissue intravital live imaging of acute surgical wounds, fibroblast lineage-specific transgenic mice, and skin-fascia explants (scar-like tissue in a dish - SCAD). We observe that injury triggers a swarming-like collective cell migration of fascia fibroblasts that progressively contracts the skin and form scars. Swarming is exclusive to fascia fibroblasts, and requires the upregulation of N-cadherin. Both swarming and N-cadherin expression are absent from fibroblasts in the upper skin layers and the oral mucosa, tissues that repair wounds with minimal scar. Impeding N-cadherin binding inhibits swarming and skin contraction, and leads to reduced scarring in SCADs and in animals. Fibroblast swarming and N-cadherin thus provide therapeutic avenues to curtail fascia mobilisation and pathological fibrotic responses across a range of medical settings.

4.
J Hazard Mater ; : 124402, 2020 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-33189469

RESUMO

Thallium (Tl) pollution caused by the exploitation of uranium (U) mines has long been neglected due to its low crustal abundance. However, Tl may be enriched in minerals of U ore because Tl has both sulfurophile and lithophile properties. Herein, a semi-dynamic leaching experiment combined with statistical analysis, geochemical speciation and multi-characterization provided novel insight into the distinct features and mechanisms of Tl release from uranium mill tailings (UMT). The results showed that particle size effects prevail over the pH on Tl release, and surface dissolution is the pivotal mechanism controlling Tl release based on Fick's diffusion model. The study revealed that long-term leaching and weathering can lead to the increased acid-extractable and oxidizable fractions of Tl in UMT, and that the exposure and dissolution of Tl-containing sulfides would largely enhance the flux of Tl release. The findings indicate that UMT containing (abundant) pyrite should be paid particular attention due to Tl exposure. Besides, critical concern over the potential Tl pollution in universal U mining and hydrometallurgical areas likewise may need to be seriously reconsidered.

5.
J Fish Dis ; 2020 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-33165930

RESUMO

Autophagy can markedly alter host response to infectious disease, and several studies have demonstrated that a restricted diet or deoxynivalenol modulates autophagy and reduces mortality of fish due to bacterial disease. The picture is less clear for viral diseases of fish. Duplicate tanks of fathead minnow, Pimephales promelas Rafinesque, were fed a replete diet (control), 100 µM chloroquine, 5 µM deoxynivalenol, 10% (fasted) or 40% of a replete diet (pair-fed) for 2 weeks and then experimentally infected by intraperitoneal injection with 2 × 105 viral haemorrhagic septicaemia virus IVb. Survival from highest to lowest for the different treatments was as follows: deoxynivalenol (average 43.3%); control (40.0%); pair-fed (35.0%); fasted (33.3%); and chloroquine (21.7%). No treatment significantly altered the survival rate of fathead minnow after VHSV IVb infection when compared to controls; however, the fish fed with chloroquine had significantly lower survival rate than the fish fed deoxynivalenol (p < .05).

6.
Environ Int ; 146: 106207, 2020 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-33197789

RESUMO

Industrial activities tend to deteriorate adjacent agricultural lands due to accumulation of potentially toxic elements in soils and crops. However, better understanding of their distinctive source partitions and transfer process remains insufficient in steel-making area. The paper focuses on the pollution levels, health risks, and provenance identification of Tl, As, Pb, Cu, Ni, Co, Sb, Cd, Zn, Be, Cr, Fe, Mn, Mo, Sn, and V in common vegetables from different farmlands near a steel-making plant. The results showed that the Tl, As, Pb, Cd, Cr, Cu and Mn were of high-level contamination in soils and generally above the maximum permissible level (MPL). Calculation using hazard quotients (HQ) exhibited that consumption of the studied vegetables may entail significant health risks to residents, especially for children, resulting from the elevated contents of Tl, As and associated toxic elements. Calculation by binary mixing model using Pb isotopic compositions suggested that steel-making activities contributed to 35-80% of the contamination of Pb and As in vegetables. It is necessary to adopt appropriate remediation measures to mitigate the farmland contamination and ensure the food safety of the agricultural products.

7.
Artigo em Inglês | MEDLINE | ID: mdl-33130226

RESUMO

STUDY OBJECTIVE: To demonstrate practical tips and tricks for successful use of the transvaginal natural orifice transluminal endoscopic surgery (NOTES) technique for performing high uterosacral ligament suspension (HUS). DESIGN: Stepwise demonstration with narrated video footage (Canadian Task Force classification III). SETTING: An academic tertiary care hospital. INTERVENTIONS: A 58-year-old G2P2, NSVDx2 with stage III anterior vaginal prolapse, stage II uterine prolapse, and posterior vaginal prolapse. The preoperative vaginal length was 7-cm. Transvaginal NOTES is a creative yet difficult approach that averts an abdominal incision while simultaneously providing enhanced visualization in comparison with traditional vaginal surgery [1]. However, this approach may be technically challenging. After performing transvaginal hysterectomy and anterior repair, the single-site port was placed, and bilateral salpingo-oophorectomy was subsequently performed. The following key techniques were used to perform NOTES-HUS: tagging the sutures for bilateral uterosacral ligament before single-site port placement, identifying the ischial spine and ureters, pulling the tagged uterosacral ligament suture to assist in locating the high uterosacral ligament, grasping and lifting the uterosacral ligament while placing a suture, and giving the suture a tug after placement to confirm the correct location [2-4]. The procedure was successfully performed in approximately 160 minutes with a postoperative vaginal length of 5-cm. Postoperative pelvic organ prolapse quantification was stage 0. CONCLUSION: Transvaginal NOTES-HUS is a feasible and practical technique for apical vaginal prolapse. There is an increased cost to using laparoscopically assisted NOTES surgery as well as a risk of pneumoperitoneum. Applying the tips and tricks presented here, such as tagging the uterosacral ligament before port placement and so on, the challenging transvaginal NOTES-HUS technique can be performed efficiently and safely.

8.
Int J Mol Sci ; 21(21)2020 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-33182266

RESUMO

Tumor suppressor p53 plays a key role in tumor suppression. In addition to tumor suppression, p53 is also involved in many other biological and pathological processes, such as immune response, maternal reproduction, tissue ischemia/reperfusion injuries and neurodegenerative diseases. While it has been widely accepted that the role of p53 in regulation of cell cycle arrest, senescence and apoptosis contributes greatly to the function of p53 in tumor suppression, emerging evidence has implicated that p53 also exerts its tumor suppressive function through regulation of many other cellular processes, such as metabolism, anti-oxidant defense and ferroptosis. Ferroptosis is a unique iron-dependent form of programmed cell death driven by lipid peroxidation in cells. Ferroptosis has been reported to be involved in cancer, tissue ischemia/reperfusion injuries and neurodegenerative diseases. Recent studies have shown that ferroptosis can be regulated by p53 and its signaling pathway as well as tumor-associated mutant p53. Interestingly, the regulation of ferroptosis by p53 appears to be highly context-dependent. In this review, we summarize recent advances in the regulation of ferroptosis by p53 and its signaling pathway. Further elucidation of the role and molecular mechanism of p53 in ferroptosis regulation will yield new therapeutic strategies for cancer and other diseases, including neurodegenerative diseases and tissue ischemia/reperfusion injuries.

9.
Reprod Sci ; 2020 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-33211273

RESUMO

BNC1 is a transcription factor that is crucial for spermatogenesis and male fertility, although the underlying mechanism remains unclear. To study BNC1's specific role in spermatogenesis, we characterized a previously developed mouse model carrying a truncating mutation in Bnc1 (termed Bnc1+/tr for heterozygotes and Bnc1tr/tr for homozygotes) and found that the mutation decreased BNC1 protein levels and resulted in germ cell loss by apoptosis. Given that loss of functional Bnc1 is known to result in decreased expression of the spermatogenesis genes Ybx2 and Papolb, we aimed to explore whether and how BNC1 promotes transcription of Ybx2 and Papolb to mediate its role in spermatogenesis. We confirmed significant reduction in YBX2 and PAPOLB protein levels in testis tissue from Bnc1+/tr and Bnc1tr/tr males compared with wild-type mice (Bnc1+/+). Consistently, knockdown of Bnc1 led to downregulation of Ybx2 and Papolb in CRL-2196 cells in vitro. To investigate if BNC1 directly induces Ybx2 and Papolb gene expression, chromatin immunoprecipitation using mouse testicular tissue and luciferase reporter assays in HEK293 cells were used to identify functional binding of BNC1 to the Ybx2 and Papolb promoters at defined BNC1 binding sites. Taken together, this study reveals a mechanism for BNC1's role in spermatogenesis by directly binding to BNC1 binding elements in the promoter regions of both Ybx2 and Papolb and inducing transcription of these important spermatogenesis genes.

10.
BMC Plant Biol ; 20(1): 457, 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33023477

RESUMO

BACKGROUND: Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food. Currently, improving starch content is one of the major goals for seed-lotus breeding. ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus. RESULTS: We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component. Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content. Seven putative AGPase genes were identified in the lotus genome (Nelumbo nucifera Gaertn.), which could be grouped into two subfamilies. Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes. Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome. NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed. CONCLUSIONS: Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed. These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content.

11.
Gen Physiol Biophys ; 39(5): 461-470, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33084599

RESUMO

Globally, acute myocardial infarction (AMI) is a serious condition affecting millions of individuals. While AMI therapy improves blood flow during surgery, reperfusion-induced injury may also occur, leading to secondary cardiac damage or even death. Here, we investigated miR-129-5p in myocardial ischemia-reperfusion (I/R) injury in rats, to explore reperfusion-related molecular mechanisms in myocardium. We used Sprague Dawley rats to establish a myocardial I/R model, with agomiR-129-5p injection, and used rat cardiomyocytes (H9c2) treated with anoxia-reoxygenation (A/R) to mimic myocardial I/R injury in vitro. A dual-luciferase reporter assay determined miR-129-5p binding to high mobility group box-1 (HMGB1) in H9c2 cells. We showed that exogenous miR-129-5p restored cardiac function indices, alleviated cardiac injury, relieved inflammatory effects and reduced infarct size and cell apoptosis in rat myocardium after I/R treatment. Elevated miR-129-5p induced a reduction in HMGB1 expression in rat I/R myocardium. miR-129-5p also targeted HMGB1, and negatively regulated its expression in H9c2 cells. Moreover, miR-129-5p overexpression in the cardiomyocytes reduced cell apoptosis and recovered cell viability after A/R injury, which was reversed by subsequent HMGB1 overexpression. These findings suggest miR-129-5p plays a cardioprotective role in ameliorating myocardial I/R injury in rats, by negatively targeting HMGB1. This mechanism provides new insights into the treatment of myocardium reperfusion-related damage.

12.
Neurology ; 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33024025

RESUMO

OBJECTIVE: To investigate the spatial patterns and the probable sequences of grey matter atrophy in spinocerebellar ataxia type 3 (SCA3). METHODS: A total of 47 patients with SCA3 and 49 age- and sex-matched healthy control subjects participated in the study. High-resolution T1-weighted magnetic resonance images were examined in all participants. We used the causal network of structural covariance (CasCN) to identify the sequence of grey matter atrophy patterns. This was achieved by applying Granger causality analysis to a grey matter atrophy staging scheme performed by voxel-based morphometry from the network level. RESULTS: Participants in the premanifest stage of the disease showed the presence of focal grey matter atrophy in the vermis. As the disease duration increased, there was progressive grey matter atrophy in the cerebellar, the neostriatum, the frontal lobe, and the parietal lobe. The patients with SCA3 also showed proximal and distal cortical atrophy sequences exerting from the vermis to the regions mainly located in the cerebellum-neostriatum-cortical network. CONCLUSION: Our results, although preliminary in nature, indicate that the grey matter atrophy in SCA3 lies and extends to involve more regions according to distinct anatomical patterns, mainly in the cerebellum-neostriatum-cortical network. These findings advance our understanding on the natural history of structural damage in SCA3, while confirming known clinical features. This could provide unique insight into the ordered sequential process of regional brain atrophy that targets a particular network.

13.
Int J Mol Med ; 46(5): 1671-1682, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33000267

RESUMO

Oxidized low­density lipoprotein (ox­LDL)­induced endothelial cell (EC) injury is a risk factor for atherosclerosis. Therefore, the present study aimed to investigate the effects of insulin­receptor substrate 1 (IRS­1) on injury to ox­LDL­exposed ECs. For this purpose, thoracic aorta tissues were isolated from rats and cultured to obtain ECs, which were then identified using immunohistochemical staining. IRS­1 overexpression plasmid (pcDNA3.1­IRS­1) and IRS­1­small interfering RNA were synthesized and transfected into ECs pre­exposed to ox­LDL. MTT and TUNEL assays were performed to evaluate the cell proliferative activity and apoptosis. Intracellular reactive oxygen species (ROS) production was determined by a flow cytometry assay. Reverse transcription­quantitative PCR was conducted to measure the peroxisome proliferator­activated receptor gamma co­activator 1 alpha (Ppargcla), phosphoenolpyruvate carboxykinase 1 (Pck1) and glucose­6­phosphatase catalytic subunit (G6pc) gene transcription levels. Western blot analysis was then performed to determine the IRS­1, forkhead box O1 (FoxO1), phosphorylated (p­)FoxO1, 78­kDa glucose­regulated protein (GRP78), p­eukaryotic translation initiation factor 2A (eIF2α), CHOP, Akt and p­Akt expression levels. Immunofluorescence staining was used to evaluate p­FoxO1 nuclear localization. The results indicated that IRS­1 significantly enhanced the proliferative activity, whereas it inhibited the apoptosis of ECs in a model of ox­LDL­induced atherosclerosis compared with ECs without IRS­1 treatment (P<0.05). IRS­1 significantly decreased the p­FoxO1/FoxO1 ratio compared with ECs without ox­LDL treatment (P<0.05). IRS­1 significantly downregulated the expression of ER stress biomarkers, including GRP78, CHOP and the p­eIF2α/eIF2α ratio in ox­LDL­exposed ECs compared with ECs without ISR­1 treatment (P<0.05). IRS­1 significantly reduced the intracellular ROS levels in the EC models of ox­LDL­induced atherosclerosis compared with ECs without IRS­1 treatment (P<0.05). Moreover, IRS­1 promoted the phosphorylation of Akt in the EC models of ox­LDL­induced atherosclerosis. IRS­1 also significantly suppressed the transcription of atherosclerosis­associated genes in ox­LDL­exposed ECs compared with ECs without IRS­1 treatment (P<0.05). Furthermore, IRS­1 significantly increased the cytoplasmic localization of p­FoxO1 in EC models of ox­LDL­induced atherosclerosis. On the whole, the findings of the present study demonstrate that IRS­1 exerts protective effects in an EC model of ox­LDL­induced atherosclerosis by inhibiting ER stress/oxidative stress­mediated apoptosis and activating the Akt/FoxO1 signaling pathway.

14.
Membranes (Basel) ; 10(10)2020 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-33007858

RESUMO

Lignification is especially prominent in postharvest pumelo fruit, which greatly impairs their attractiveness and commercial value. This study investigated the energy metabolism and lipid peroxidation and their relationship with accumulated lignin content in juice sacs of "Hongroumiyou" (HR) during 90 d of storage at 25 °C. The results indicated that, the alterations of energy metabolism in juice of sacs of postharvest pumelos was featured by a continuous decline in energy charge and ATP/ADP; an increase in succinic dehydrogenase (SDH) activity before 30 d and increases in activities of cytochrome c oxidase (CCO) and F0F1-ATPase before 60 d; but declines in activities of Ca2+-ATPase and H+-ATPase. Additionally, enhanced contents of H2O2, O2-, and -OH scavenging rate; increased malondialdehyde (MDA) content; and transformation of unsaturated fatty acids (USFA) to saturated fatty acids (USFA) and reduced USFA/SFA (U/S) could result in lipid peroxidation and membrane integrity loss. Moreover, correlation analysis showed that lignin accumulation was in close relation to energy metabolism and lipid peroxidation in juice sacs of postharvest pumelos. These results gave evident credence for the involvement of energy metabolism and lipid peroxidation in the lignin accumulation of HR pumelo fruit during postharvest storage.

15.
Pediatr Infect Dis J ; 2020 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-33021592

RESUMO

OBJECTIVE: The purpose of the current study is to investigate the bactericidal effect of macrolides and ß-lactams on Bordetella pertussis (B. pertussis) in the nasopharynx and provide guidance for treating macrolides-resistant B. pertussis infections. METHODS: Patients with whooping cough was diagnosed by culture of nasopharynx swabs between January 2016 to December 2018. B. pertussis was identified using specific antisera against pertussis and parapertussis. Drug susceptibility test was carried out using the E-test method. The clearance of B. pertussis in nasopharynx at 7 and 14 days into and posttreatment with macrolides, and ß-lactams was compared. RESULTS: A total of 125 B. pertussis samples were collected from patients who received single antibiotic treatment. Among those isolates, 62.4% (78/125) had high resistance with minimum inhibitory concentrations greater than 256 mg/L for erythromycin and azithromycin. The MIC90 of piperacillin, cefoperazone-sulbactam, meropenem, ampicillin, ceftriaxone, ceftazidime and trimethoprim-sulfamethoxazole for these isolates was <0.016, 0.094, 0.094, 0.19, 0.19, 0.25 and 0.75 mg/L, respectively. The clearance rate with ß-lactams treatment (68.8%, 44/64) was significantly higher than that with macrolides treatment at 14 days posttreatment (50.8%, 31/61) (χ = 4.18, P = 0.04). Macrolides had a better clearance rate at 7 days posttreatment than ß-lactams (χ = 4.49, P = 0.03) for macrolides-sensitive isolates and a worse clearance rate for macrolides-resistant isolates. CONCLUSION: B. pertussis isolates had a high-resistant rate for macrolides in our study. Macrolides are the first choice for treating pertussis caused by macrolides-sensitive strains, and some ß-lactams such as piperacillin should be considered as alternative antibiotics for treatment of macrolides-resistant B. pertussis infection.

16.
Psychol Health Med ; : 1-6, 2020 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-33080151

RESUMO

Medical staff in radiology departments faces a higher risk of infection and a heavier workload during the new coronavirus disease (COVID-19) outbreak. High perceived stress levels endanger physical and mental health and affect work efficiency and patient safety. Therefore, it is urgent to understand the perceived stress levels of medical staff and explore its risk factors. We recruited 600 medical staff from the radiology departments of 32 public hospitals in Sichuan Province, China, to evaluate perceived stress scores via a mobile app-based questionnaire. The results showed that the perceived stress level among medical staff in the radiology departments during the COVID-19 outbreak was high and a sense of tension was strongly present. A positive correlation was found between anxiety score and perceived stress. Multivariate analysis showed that risk factors for perceived stress were female, existing anxiety, and fears of being infected at work, an uncontrollable outbreak, and not being able to pay rent or mortgage. Conversely, good knowledge about COVID-19, being unmarried, and working in a higher-grade hospital were protective factors for perceived stress. Therefore, more attention should be given to medical staff in the radiology departments that present the risk factors outlined above. Timely risk assessment of psychological stress and effective intervention measures should be taken for these high-risk groups to keep their perceived stress within normal limits.

17.
Artigo em Inglês | MEDLINE | ID: mdl-33107573

RESUMO

BACKGROUND: Ultrasound guided thermal ablation plays an important role in the management of thyroid disease. The objective of this study was to evaluate the feasibility, efficacy, and safety of thermal ablation for patients with solitary T1bN0M0 PTC who are ineligible for or unwilling to undergo surgery. MATERIALS AND METHODS: Data pertaining to 172 patients (38 males and 134 females) who received thermal ablation therapy at 12 hospitals between April 2015 and March 2020 were retrospectively analyzed. The mean duration of follow-up was 24.9 ± 14.1 months (range, 12-60). The technical feasibility, technical success, efficacy, and safety of treatment were analyzed. Post-ablation tumor size at various time-points was compared with pre-ablation measurement. RESULTS: All patients selected for thermal ablation received enlarged ablation according to contrast-enhanced ultrasound post-ablation. The maximum diameter and volume of ablation zone at 6,12, 18, 24, 36, and 48 months post-ablation were significantly smaller than those recorded pre-ablation (P < 0.05 for all). At the most recent follow-up, 106 (61.6%) tumors had completely disappeared. The rate of lymph node metastasis (LNM) was 0.6% (1/172) and the incidence of new tumor was 1.2% (2/172). The overall complication rate was 5.2% (9/172) (major complications: 4.6% [8/172]; minor complications: 0.6% [1/172]). All major complications were relieved within four months post-ablation. CONCLUSION: Thermal ablation may be a feasible, effective, and safe treatment option for patients with solitary T1bN0M0 PTC who are ineligible for or unwilling to undergo surgery. It may provide a novel treatment option for selected patients.

19.
Artigo em Inglês | MEDLINE | ID: mdl-33096264

RESUMO

STUDY OBJECTIVE: To demonstrate stepwise techniques for the successful utilization of the LESS technique for safely performing pectopexy. DESIGN: Stepwise demonstration with narrated video footage (Canadian Task Force classification III). SETTING: An academic tertiary care hospital. PATIENT: A 48-year-old G2P2, NSVDx2 with Stage III anterior vaginal prolapse, Stage III uterine prolapse & posterior vaginal prolapse. The preoperative vaginal length was 6 cm. INTERVENTIONS: Laparoscopic sacrocolpopexy is the current gold standard for pelvic organ prolapse demonstrating a low recurrence rate; however, it can be technically challenging to perform, particularly in women with obesity, or in the event of an anatomical variation. The pectineal ligament, also known as Cooper's ligament, is familiar to surgeons and can be utilized for a tension free mesh suspension in prolapse patients. Integration of Laparoendoscopic single site surgery (LESS) and pectopexy is a novel alternative minimally invasive approach that is more cosmetic, simpler and effective. Key steps in LESS pectopexy include:Measurements and main results: The procedure was successfully performed in approximately 80 minutes with a postoperative vaginal length of 6 cm. Postoperative pelvic organ prolapse quantification was stage 0. CONCLUSION: Laparoendoscopic single site surgery (LESS) is a feasible technique for pectopexy in patients with pelvic organ prolapse. LESS pectopexy results in better cosmesis and offers an alternative for challenging pelvic organ prolapse patient such as obesity.

20.
Biol Reprod ; 2020 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-33074310

RESUMO

MicroRNA (miR)-210 is a well-known hypoxia-inducible small RNA. Increasing in vitro evidence demonstrate its involvement in regulating multiple behaviors of placental trophoblasts. However, direct in vivo evidence remains lacking. In the present study, we generated a miR-210-deficient mouse strain using CRISPR/Cas9 technology, in which miR-210 expression was markedly deficient in various tissues. Little influence on fertility rate and litter size was observed after the deletion of miR-210 in mice. Continuous exposure of pregnant mice to hypoxia (10.5% O2) from E6.5 to E10.5 or to E18.5 led to reduction in fetal weight, and such fetal weight loss was markedly worsened in miR-210-knockout dams. Analysis of the placental structure demonstrated the reduced expansion of placental spongiotrophoblast layer and hampered development of labyrinth fetal blood vessels in knockout mice compared to the wildtype controls upon hypoxia stimulation. The findings indicate that miR-210 participates in regulating placental adaptation to hypoxic stress during pregnancy.

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