Size-matched hierarchical porous carbon materials anchoring single-atom Fe-N4 sites for PMS activation: An in-depth study of key active species and catalytic mechanisms.

Single-atom catalysts are considered to be one of the most promising catalysts for AOPs. However, how to design and synthesize cost-effective and highly loaded single-atom catalysts is the bottleneck limiting its development and application. In this study, we report a highly loaded single-atom iron catalyst (Fe-SAC-BC) using waste biomass as a carbon carrier to anchor Fe-N4 sites. The catalyst showed excellent catalytic performance and stability in wastewater treatment. Unlike conventional radical oxidation, the non-radical degradation process of Fe-N4 as the active site and high-valent iron-oxygen intermediates as the key active species identified by burst and probe experiments. DFT calculations and molecular dynamics simulations were applied to the catalytic mechanism of Fe-SAC-BC, in which Fe (III)-N4 is the most likely active site and Fe (IV)-OH is the most dominant active species. This study provides new strategies and understanding for the design of novel single-atom catalysts and the mechanistic probing of the non-radical pathways of AOPs.

Optimization of a QuEChERS-LC-MS/MS method for 51 pesticide residues followed by determination of the residue levels and dietary intake risk assessment in foodstuffs.

A modified QuEChERS purification-liquid chromatography-tandem mass spectrometry method has been developed to determine 51 pesticide residues with newly established maximum residue limits (MRLs) in foodstuffs. Samples were extracted with acetonitrile under citrate-buffered conditions and purified using a modified QuEChERS method employing hydroxylated MWCNTs, SAX, and C18. The limits of quantification ranged from 0.2 to 9.8 µg/kg. Recoveries in ten different foodstuffs ranged from 70.2% to 117.9%, with relative standard deviations between 2.3% and 19.9% at three spiking levels. This method was applied to analyze 352 market samples, detecting 14 pesticides in 97 samples. Notably, Afidopyropen, cyantraniliprole, and fluxapyroxad residues in vegetables exhibited a consistent pattern of higher levels in the spring and winter and lower levels in the summer and autumn. Moreover, the risk assessments for acute and chronic dietary exposure to the 14 detected pesticides indicated that the %ADI and %ARfD were well below 100%.

Recent advancements in the taste transduction mechanism, identification, and characterization of taste components.

In the realm of human nutrition, the phenomenon known as taste refers to a distinctive sensation elicited by the consumption of food and various compounds within the oral cavity and on the tongue. Moreover, taste affects the overall comfort in the oral cavity, and is a fundamental attribute for the assessment of food items. Accordingly, clarifying the material basis of taste would be conducive to deepening the cognition of taste, investigating the mechanism of taste presentation, and accurately covering up unpleasant taste. In this paper, the basic biology and physiology of transduction of bitter, umami, sweet, sour, salty, astringent, as well as spicy tastes are reviewed. Furthermore, the detection process of taste components is summarized. Particularly, the applications, advantages, and distinctions of various isolation, identification, and evaluation methods are discussed in depth. In conclusion, the future of taste component detection is discussed.

TMT quantitative proteomics analysis reveals molecular mechanism of ferroptosis during beef refrigeration.

Ferroptosis is a recently identified cell death process in refrigerated beef, and its mediated protein oxidation and cell death may reduce muscle quality, but the mechanism of ferroptosis is unclear. In the study, free iron accumulation reached 19.670 ± 0.482 µg/g after 6 days refrigeration, the levels of apoptosis, ROS, and lipid peroxidation increased significantly (P < 0.05), and muscle tissue cells exhibited typical ferroptosis characteristics. A total of 377 differentially expressed proteins (DEPs) were identified by TMT quantitative proteomics. 15 DEPs, including transferrin, ferritin, glutathione peroxidase (GPX) 4, and heme oxygenase 1, were involved in lipid peroxidation, Fe2+ and Fe3+ conversion, iron ion accumulation, and mitochondrial oxidative stress to induce ferroptosis. In addition, signalling pathways, such as chemical carcinogenesis-ROS, glutathione metabolism, HIF-1, and PPAR may promote ferroptosis by affecting free iron overload and GPX4 inactivation.

Development of a fluorescence-based sensor based on 1,8-naphthalimide for highly sensitive detection of phosgene.

Developing advanced sensing technologies for phosgene is critical for public safety and national security. Here, we report a novel fluorescent probe NP (abbreviation for naphthalimide-based probe) for the selective detection of phosgene. The sensor was synthesized through a substitution reaction between 1,8-naphthalimide with 1,4,7-triazacyclononane. The high reactivity of probe NP towards phosgene led to a marked fluorescence emission turn-on, demonstrating the high selectivity of this sensor. Furthermore, a practical on-site detection method for phosgene was developed by loading probe NP on test strips. These test strips can detect gaseous phosgene below 1 ppm. The successful development of this sensor opens up new possibilities for detecting methods for phosgene.

Fermentation-promoting effect of three salt-tolerant Staphylococcus and their co-fermentation flavor characteristics with Zygosaccharomyces rouxii in soy sauce brewing.

Staphylococcus is the dominant genus in the fermentation process of soy sauce, but its effect on the flavor of soy sauce has not been clearly established. In order to investigate the role of this genus in soy sauce fermentation, individual fermentation with Staphylococcus spp. screened from the moromi and their co-fermentation with an ester-producing yeast of Zygosaccharomyces rouxii were designed. Through the analysis of physicochemical properties, organic acid composition, volatile flavor compounds (VFCs) and sensory characteristics during fermentation, Staphylococcus was confirmed as a contributor to the acidity, ester aroma and alcohol aroma of soy sauce. In their co-fermentation with yeast, the ester aroma of soy sauce was further enhanced. Moreover, pathway enrichment analysis and network construction of key VFCs also revealed potential metabolic networks for formation of characteristic flavor compounds in co-fermentation. This work will help optimize the fermentation functional microbiota to obtain better soy sauce flavor.

New electric field responsive photonic crystals with remarkable yellow-to-green switch for adaptive camouflage.

Electric field responsive photonic crystals take on critical significance in developing adaptive camouflage technology, which are promising materials for adaptive camouflage devices with better fabrication processes and color saturation. However, electric field responsive photonic crystals are primarily susceptible to poor fusion with typical background colors and necessitate the continuous implementation of electric fields to attain specific colors, thus limiting their practical applications. Monodisperse polyvinylpyrrolidone modified cadmium sulfide (PVP/CdS) microspheres with large refractive index are well prepared in this study. Liquid photonic crystals, exhibiting earth-yellow and light green under specific electric field, are obtained with PVP/CdS particles dispersed in propylene carbonate in accordance with the subtractive color mixing principle of structural color and initial color. The resulting electric field responsive photonic crystals are characterized by a simple preparation process, fast electrical response, long-time holding of the earth-yellow state, and reversible color changes between earth-yellow and light green in -3.5 V and + 3.5 V electric field switching. This study can contribute to the development of color-changing devices designed for adaptive camouflage applications.

Dual Z-scheme TCN/ZnS/ ZnIn2S4 with efficient separation for photocatalytic nitrogen fixation.

The development of an efficient catalyst that can use solar energy for NH3 production is of great significance in solving the environmental and energy crisis caused by the traditional ammonia synthesis process. In this work, a dual Z-scheme tubular carbon nitride/zinc sulfide/zinc indium sulfide ternary composited photocatalyst (TCN/ZnS/ZnIn2S4) with excellent nitrogen photofixation performance under visible light was prepared by self-assembly and hydrothermal methods. The crystal structure studies confirmed that tubular carbon nitride (TCN) had more active sites that could promote N2 adsorption. The photochemical studies proved that the double charge transfer channel provided by the dual Z-scheme heterojunction could improve the efficiency of electron-hole separation and achieve excellent photocatalytic nitrogen fixation. The ammonia production rate of the TCN/ZnS/ZnIn2S4 catalyst was up to 136.56 µmol/L, and it also has good stability and reusability. This work provides new insight into the development of Z-scheme heterojunction photocatalysts with green and efficient nitrogen fixation.

iTRAQ-based proteomics identifies proteins associated with betaine accumulation in Lycium barbarum L.

In order to better understand the mechanism of betaine accumulation in Lycium barbarum L. (LBL), we used iTRAQ (Isotope relative and absolute quantitative labeling) proteomics to screen and identify differentially abundant proteins (DAPs) at five stages (S1-young fruit stage, S2-green fruit stage, S3-early yellowing stage, S4-late yellowing stage, S5-ripening stage). A total of 1799 DAPs and 171 betaine-related DAPs were identified, and phosphatidylethanolamine N-methyltransferase (NMT), choline monooxygenase (CMO), and betaine aldehyde dehydrogenase (BADH) were found to be the key enzymes related to betaine metabolism. These proteins are mainly involved in carbohydrates, amino acids and their derivatives, fatty acids, carboxylic acids, photosynthesis and photoprotection, isoquinoline alkaloid biosynthesis, peroxisomes, and glycine, serine, and threonine metabolism. Three of the key enzymes were also up- and down-regulated to different degrees at the mRNA level. The study provide new insights into the of mechanism of betaine accumulation in LBL. SIGNIFICANCE: Betaine, a class of naturally occurring, water-soluble alkaloids, has been found to be widespread in animals, higher plants, and microbes. In addition to being an osmotic agent, betaine has biological functions such as hepatoprotection, neuroprotection, and antioxidant activity. Betaine metabolism (synthesis and catabolism) is complexly regulated by developmental and environmental signals throughout the life cycle of plant fruit maturation. As a betaine-accumulating plant, little has been reported about the regulatory mechanisms of betaine metabolism during the growth and development of Lycium barbarum L. (LBL) fruit. Therefore, this study used iTRAQ quantitative proteomics technology to investigate the abundance changes of betaine-related proteins in LBL fruit, screen and analyze the differential abundance proteins related to betaine metabolism, and provide theoretical references for the in-depth study of the mechanism of betaine metabolism in LBL fruit.

Potential Mechanisms Underlying the Therapeutic Roles of Gancao fuzi Decoction in Cold-dampness Obstruction Syndrome-type Knee Osteoarthritis.

BACKGROUND: The key active components and potential molecular mechanism of Gancao Fuzi decoction (GFD) in the treatment of cold-dampness obstruction-type knee osteoarthritis (KOA) remain unclear. OBJECTIVES: To explore the mechanism of GFD in the treatment of cold-dampness obstruction syndrome-type KOA by network pharmacology. METHODS: The potential active components and targets of the four herbs in GFD (Fuzi, Guizhi, Baizhu, and Gancao) were screened using the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database. The targets of KOA were obtained with the Comparative Toxicogenomics Database (CTD), the GeneCards database, and the DisGeNET database, and the common targets of the drugs and disease were ultimately obtained. Cytoscape (v.3.7.1) was used to draw the active component-target network, and the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) (v.11.0) database was used to construct the protein interaction network. The Database for Annotation, Visualization, and Integrated Discovery (DAVID) was used for the Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of the intersecting targets. RESULTS: A total of 102 potential active components and 208 targets of GFD in the treatment of cold-dampness obstruction syndrome-type KOA were screened. GFD treatment was found to be closely related to many inflammatory signalling pathways in the treatment of KOA. CONCLUSION: The effect of GFD on cold-dampness obstruction syndrome-type KOA is mediated by multicomponent, multitarget, and multichannel mechanisms, which provides the basis for further experimental study of its pharmacodynamic material basis and mechanism.

Structure-activity relationships of bioactive polysaccharides extracted from macroalgae towards biomedical application: A review.

Macroalgae are valuable and structurally diverse sources of bioactive compounds among marine resources. The cell walls of macroalgae are rich in polysaccharides which exhibit a wide range of biological activities, such as anticoagulant, antioxidant, antiviral, anti-inflammatory, immunomodulatory, and antitumor activities. Macroalgae polysaccharides (MPs) have been recognized as one of the most promising candidates in the biomedical field. However, the structure-activity relationships of bioactive polysaccharides extracted from macroalgae are complex and influenced by various factors. A clear understanding of these relationships is indeed critical in developing effective biomedical applications with MPs. In line with these challenges and knowledge gaps, this paper summarized the structural characteristics of marine MPs from different sources and relevant functional and bioactive properties and particularly highlighted those essential effects of the structure-bioactivity relationships presented in biomedical applications. This review not only focused on elucidating a particular action mechanism of MPs, but also intended to identify a novel or potential application of these valued compounds in the biomedical field in terms of their structural characteristics. In the last, the challenges and prospects of MPs in structure-bioactivity elucidation were further discussed and predicted, where they were emphasized on exploring modern biotechnology approaches potentially applied to expand their promising biomedical applications.

Developing a novel benzothiazole-based red-emitting probe for intravital imaging of superoxide anion.

Superoxide anion (O2•-), the first generated reactive oxygen species (ROS), is a critical player in cellular signaling network and redox homeostasis. Imaging of O2•-, particularly in vivo, is of concern for further understanding its roles in pathophysiological and pharmacological events. Herein, we designed a novel probe, (E)-4-(5-(2-(benzo[d]thiazol-2-yl)-2-cyanovinyl)furan-2-yl)phenyl trifluoromethane-sulfonate (BFTF), by modifying hydroxyphenyl benzothiazole (a widely used dye scaffold) which includes insertion of both an acrylonitrile unit and a furan ring to extend the total π-conjugation system and to enhance push-pull intramolecular charge transfer process, and utilization of trifluoromethanesulfonate as the response unit. Toward O2•-, the probe features near-infrared fluorescent emission (685 nm), large Stokes shift (135 nm), and deep tissue penetration (300 µm). With its help, we successfully mapped preferential generation of O2•- in HepG2 cells over L02 cells, as well as in A549 over BEAS-2B cells by ß-lapachone (an anticancer agent that generates O2•-), and more importantly, visualized overproduction of O2•- in living mice with liver injury induced by acetaminophen (a well-known analgesic and antipyretic drug).

The essential roles of m6A modification in osteogenesis and common bone diseases.

N6-methyladenosine (m6A) is the most prevalent modification in the eukaryotic transcriptome and has a wide range of functions in coding and noncoding RNAs. It affects the fate of the modified RNA, including its stability, splicing, and translation, and plays an important role in post-transcriptional regulation. Bones play a key role in supporting and protecting muscles and other organs, facilitating the movement of the organism, ensuring blood production, etc. Bone diseases such as osteoarthritis, osteoporosis, and bone tumors are serious public health problems. The processes of bone development and osteogenic differentiation require the precise regulation of gene expression through epigenetic mechanisms including histone, DNA, and RNA modifications. As a reversible dynamic epigenetic mark, m6A modifications affect nearly every important biological process, cellular component, and molecular function, including skeletal development and homeostasis. In recent years, studies have shown that m6A modification is involved in osteogenesis and bone-related diseases. In this review, we summarized the proteins involved in RNA m6A modification and the latest progress in elucidating the regulatory role of m6A modification in bone formation and stem cell directional differentiation. We also discussed the pathological roles and potential molecular mechanisms of m6A modification in bone-related diseases like osteoporosis and osteosarcoma and suggested potential areas for new strategies that could be used to prevent or treat bone defects and bone diseases.

The 2022 report of synergetic roadmap on carbon neutrality and clean air for China: Accelerating transition in key sectors.

China is now confronting the intertwined challenges of air pollution and climate change. Given the high synergies between air pollution abatement and climate change mitigation, the Chinese government is actively promoting synergetic control of these two issues. The Synergetic Roadmap project was launched in 2021 to track and analyze the progress of synergetic control in China by developing and monitoring key indicators. The Synergetic Roadmap 2022 report is the first annual update, featuring 20 indicators across five aspects: synergetic governance system and practices, progress in structural transition, air pollution and associated weather-climate interactions, sources, sinks, and mitigation pathway of atmospheric composition, and health impacts and benefits of coordinated control. Compared to the comprehensive review presented in the 2021 report, the Synergetic Roadmap 2022 report places particular emphasis on progress in 2021 with highlights on actions in key sectors and the relevant milestones. These milestones include the proportion of non-fossil power generation capacity surpassing coal-fired capacity for the first time, a decline in the production of crude steel and cement after years of growth, and the surging penetration of electric vehicles. Additionally, in 2022, China issued the first national policy that synergizes abatements of pollution and carbon emissions, marking a new era for China's pollution-carbon co-control. These changes highlight China's efforts to reshape its energy, economic, and transportation structures to meet the demand for synergetic control and sustainable development. Consequently, the country has witnessed a slowdown in carbon emission growth, improved air quality, and increased health benefits in recent years.

Effects of different strains fermentation on the sensory and nutritional properties of soy-based yogurt.

BACKGROUND: Soy protein is the only full plant protein that is comparable to animal protein. Soy whey contains a variety of nutrients including isoflavones and oligosaccharides. Additionally, different strains have profound influence on functional metabolism. Most prospective studies used mixed strain fermented yogurt, but few studies on single strain fermented yogurt. The present study aimed to ferment nutritious and healthy soy-based yogurt using a single strain and to provide technical support for the reuse of soy whey. RESULTS: Streptococcus salivarius subsp. thermophilus HCS07-002, Bifidobacterium animalis subsp. lactis HCS04-001 and Lactiplantibacillus plantarum HCS03-084 had strong growth and metabolic activities. Bifidobacterium animalis subsp. lactis HCS04-001 fermentation raised the texture and rheological properties of yogurt and enriched the flavor substances in the yogurt, resulting in a good quality soy-based yogurt. The contents of arginine, glutamic and proline were greatly affected by the fermentation of different strains. The fermentation of B. animalis subsp. lactis HCS04-001 was beneficial to the enrichment of soy isoflavone and equol in yogurt, and enhanced antioxidant activity of yogurt. CONCLUSION: Our findings indicated that yogurt fermented with B. animalis subsp. lactis HCS04-001 had the best sensory and nutritional properties, which provides valuable insights into the selection of suitable strains to improve the quality and nutritional value of plant yogurt. © 2023 Society of Chemical Industry.

Associations of nighttime light exposure during pregnancy with maternal and neonatal gut microbiota: A cohort study.

BACKGROUND: Nighttime light (NTL) pollution has been reported as a risk factor for human health. However, the relationship between NTL and gut microbiota has not been reported in pregnant women and neonates. This study was conducted to investigate the relationship between NTL and gut microbial diversity and composition in mothers and their neonates. METHODS: This study analyzed 44 mothers and 28 newborns. The composition of gut microbiota was evaluated using 16S rRNA V3-V4 sequencing. The monthly mean NTL exposure during pregnancy was respectively calculated based on each participant's residential address (NTLpoint) and a concentric 1 km radius buffer zone around their address (NTL1000m). The relationships between NTL exposure and gut microbiota of mothers and newborns were assessed using generalized linear models. RESULTS: NTL exposure during pregnancy was not associated with alpha diversity of mothers or neonates. For mothers, results revealed that after adjusting for covariates, NTLpoint was negatively correlated with Prevotella_2 (p = 0.004, FDR-adjusted p = 0.030) and norank_o__Gastranaerophilales (p = 0.018, FDR-adjusted p = 0.049) at the genus level. In addition, Lachnospira (p = 0.036, FDR-adjusted p = 0.052) and Coprococcus_3 (p = 0.025, FDR-adjusted p = 0.052) were positively correlated with NTLpoint. The association between Coprococcus_3 (p = 0.01, FDR-adjusted p = 0.046) and NTLpoint persisted even after controlling for covariates. For neonates, Thauera was positively associated with NTLpoint (p = 0.015) and NTL1000m (p = 0.028), however, after adjusting for covariates and FDR correction, Thauera was not significantly associated with NTLpoint and NTL1000m. CONCLUSIONS: This study found that NTL exposure was associated with maternal gut microbiota composition. Our findings provide a foundation for the potential impact of NTL exposure on maternal gut microbiota from a microbiological perspective. More population-based validation of the effects of NTL exposure on human gut microbiota is needed in future.

Interplay between SUMO1-related SUMOylation and phosphorylation of p65 promotes hepatocellular carcinoma progression.

The nuclear factor kappaB (NF-κB) subunit p65, plays an important role in the progression of hepatocellular carcinoma (HCC). Phosphorylation of p65 is considered as an important mechanism for the positive regulation of NF-κB activity. According to our previous data, p65 can be SUMOylated by small ubiquitin-related modifier 1 (SUMO1) protein, and SUMO1 promotes p65 nuclear import and HCC progression. However, the effect of SUMO1-related p65 SUMOylation on NF-κB transcriptional activity and the relationship between phosphorylation and SUMOylation of p65 remain obscure. Here, we found that phosphorylated p65 level was increased in cancer tissues of HCC patients, and similar phenomenon was found for SUMO1 expression but not for SUMO2/3. Further clinical data showed a positive correlation between SUMO1 and phosphorylated p65. We also verified that overexpression of SUMO1 upregulated phosphorylated p65 levels. Next, we verified SUMO1-related p65 SUMOylation with in vitro SUMOylation assay, constructed mutants of p65 SUMOylation and phosphorylation, and found that SUMO1-related p65 SUMOylation promoted p65 nuclear import and increased NF-κB activity. Both SUMO1-related p65 SUMOylation and p65 phosphorylation (especially at S276 site) increased the viability and invasion of hepatoma cells, and decreased the apoptosis of hepatoma cells. At last, we found that the phosphorylation of p65 promoted the level of SUMO1-related p65 SUMOylation, and SUMO1-related p65 SUMOylation upregulated phosphorylated p65 (at S276 site). Our study contributes to the exploration of the oncogenic mechanism of p65, which is the important protein in HCC.

Effect of macrophage polarization on parasitic protection against type 1 diabetes mellitus.

Type 1 diabetes mellitus is a chronic disease caused by the destruction of pancreatic beta cells. Based on the hygiene hypothesis, a growing body of evidence suggests a negative association between parasitic infections and diabetes in humans and animal models. The mechanism of parasite-mediated prevention of type 1 diabetes mellitus may be related to the adaptive and innate immune systems. Macrophage polarization is a new paradigm for the treatment of type 1 diabetes mellitus, and different host macrophage subsets play various roles during parasite infection. Proinflammatory cytokines are released by M1 macrophages, which are important in the development of type 1 diabetes mellitus. Parasite-activated M2 macrophages prevent the development of type 1 diabetes mellitus and can influence the development of adaptive immune responses through several mechanisms, including Th2 cells and regulatory T cells. Here, we review the role and mechanism of macrophage polarization in parasitic protection against type 1 diabetes mellitus.

Region-specific hippocampal atrophy is correlated with poor glycemic control in type 2 diabetes: a cross-sectional study.

To examine the association between prediabetes/type 2 diabetes mellitus (T2DM) and hippocampal subfields and to investigate the effects of glycemic control (HbA1c and FBG)/diabetes duration on the volume of hippocampal subfields in T2DM patients. This cross-sectional study included 268 participants from Tianjin Union Medical Center between August 2019 and July 2022. The participants were divided into three groups: T2DM, prediabetes and no diabetes. All participants underwent brain MRI examination on a 3T MRI scanner. FreeSurfer was performed to segment hippocampus automatically based on T1 MPRAGE images. The relationships between glycemic status/glycemic control/diabetes duration and hippocampal subfield volumes were estimated by multiple linear regression analysis/generalized additive modeling (GAM). Among all participants, 76 (28.36%) had prediabetes, and 96 (35.82%) had T2DM. In multi-adjusted linear regression models, those with prediabetes had a significantly lower volume of bilateral parasubiculum (ßright = -5.540; ßleft = -6.497). Those with diabetes had lower volume of parasubiculum (ßleft = -7.868), presubiculum-head (ßleft = -6.244) and fimbria (ßleft = -7.187). We did not find relationship between diabetes duration and hippocampal subfield volumes. In stratified analysis, long duration with high FBG related with lower volume of right fimbria (ßright = -15.583). Long duration with high HbA1c related with lower volume of presubiculum-head (ßright = -19.693), subiculum-head (ßright = -28.303), subiculum-body (ßleft = -38.599), CA1-head (ßright = -62.300, ßleft = -47.922), CA1-body (ßright = -19.043), CA4-body (ßright = -14.392), GC-ML-DG-head (ßright = -20.521), GC-ML-DG-body (ßright = -16.293, ßleft = -12.799), molecular_layer_HP-head (ßright = -44.202, ßleft = -26.071) and molecular_layer_HP-body, (ßright = -31.368), hippocampal_tail (ßleft = -80.073). Prediabetes related with lower bilateral parasubiculum volume, and T2DM related with lower left parasubiculum, presubiculum-head and fimbria. T2DM with chronic poor glycemic control had lower volume in multiple hippocampal subregions.