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1.
Ecotoxicol Environ Saf ; 213: 112036, 2021 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-33588187

RESUMO

A hydroponic method was performed to explore the effects of sulfate supply on the growth, manganese (Mn) accumulation efficiency and Mn stress alleviation mechanisms of Polygonum lapathifolium Linn. Three Mn concentrations (1, 8 and 16 mmol L-1, representing low (Mn1), medium (Mn8) and high (Mn16) concentrations, respectively) were used. Three sulfate (S) levels (0, 200, and 400 µmol L-1, abbreviated as S0, S200 and S400, respectively) were applied for each Mn concentration. (1) The average biomass (g plant-1) of P. lapathifolium was ordered as Mn8 (6.36) > Mn1 (5.25) > Mn16 (4.16). Under Mn16 treatment, S addition increased (P < 0.05) biomass by 29.96% (S200) and 53.07% (S400) compared to that S0. The changes in the net photosynthetic rate and mean daily increase in biomass were generally consistent with the changes in biomass. (2) Mn accumulation efficiency (g plant-1) was ordered as Mn8 (99.66) > Mn16 (58.33) > Mn1 (27.38); and S addition increased (p < 0.05) plant Mn accumulation and Mn transport, especially under Mn16 treatment. (3) In general, antioxidant enzyme activities (AEAs) and malondialdehyde (MDA) in plant leaves were ordered in Mn16 > Mn8 > Mn1. Sulfate addition decreased (P < 0.05) AEAs and MDA under Mn16 treatment, while the changes were minor under Mn1 and Mn8 treatments. (4) Amino acid concentrations generally increased with increasing Mn concentration and S level. In summary, the medium Mn treatment promoted plant growth and Mn bioaccumulation; sulfate, especially at 400 µmol L-1 S, can effectively promote plant growth and Mn accumulation efficiency. The most suitable bioremediation strategy was Mn16 with 400 µmol L-1 S.

2.
Artigo em Inglês | MEDLINE | ID: mdl-33483923

RESUMO

Landscape plants have both ecological and aesthetic value and may also represent ideal candidates for phytoremediation. In the present study, one round of hydroponic culture for 14 days with different cadmium (Cd) concentrations (0, 0.5, 1, and 2 mg L-1 Cd) was carried out to test whether Hydrocotyle vulgaris L. is a Cd-tolerant plant. Furthermore, physiological parameters, including pigment concentrations, photosynthesis, antioxidant enzyme activities (AEAs), and nutrient uptake, were also examined to determine the tolerance of H. vulgaris to Cd exposure. The results showed that H. vulgaris could grow normally under all Cd supply levels. The Cd removal efficiency reached 100% at Cd concentrations ≤1.0 mg L-1. The concentrations of Cd in roots and shoots increased (P < 0.05) with Cd supplementation. The maximum concentrations of Cd reached 26.4 and 118 mg kg-1 in shoots and roots, respectively. The translocation factor values were similar under all Cd treatments. The highest mean daily increase in biomass (MDIB) was obtained under 1 mg L-1 Cd exposure, which increased by 69.86% compared to that in the control, which may be due to the increased photosynthetic pigments, photosynthetic rate, and the consistent nutrient concentrations under this Cd level, as there were positive relationships between these parameters and MDIB. Moreover, the activities of AEA also generally explicated highest among all Cd levels. All these results indicate that the above physiological parameters play a positive role in promoting plant growth and alleviating Cd stress. In summary, H. vulgaris was verified as a potential Cd-tolerant plant, providing new information for Cd phytoremediation. Furthermore, given its extensive habitat distribution, this species might be tested for phytoremediation of contaminated soils in future work.

3.
Talanta ; 222: 121524, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33167234

RESUMO

Protein MALDI imaging mass spectrometry (MALDI-IMS) holds a great promise to acquire spatial distribution information of proteins on biological tissue, but it suffers from the small number of proteins detected by direct MALDI-IMS detection. Ionic surfactants have been extensively used for protein extraction to improve the number of proteins detected in tissue samples by LC-MS analysis, but seldom by direct MALDI-IMS detection. Nonionic surfactants are milder than ionic surfactants and protein native structures are remained after extraction, which favors the spatial resolution of direct MALDI-IMS. However, nonionic surfactants are less effective than ionic surfactants. In this report, we utilized polyoxyethylene nonionic surfactants (PNS) to pre-incubate the tissue section, followed by the on-tissue trypsin digestion and then direct MALDI detection of in-situ formed peptides. For the first time, we observed that the additive effect of PNS and the synergistic effect of the mixed PNS in improving the number of peptides detected. Specifically, the peptides detected were 73.0-90.7% distinct when the different PNS (Tween 80 or Triton X-100 alone or their mixture) was used. Taking advantage of this additive effect, the 96 proteins including 12 transmembrane proteins were detected, corresponding to a ~10-fold improvement compared to MALDI-IMS without surfactant. When the mixed surfactants were used to replace Tween 80 and Triton X-100 alone, the optimized surfactant concentration decreased 20-100-fold and the number of peptides detected with m/z > 2500 Da was improved 15-fold. The additive and synergistic effects of PNS suggested that the interaction mode between each PNS and proteins is highly variable. Benefiting from the strong additive effect and diversity of PNS, further improvement of the number of proteins detected by MALDI-IMS is clearly feasible.

4.
J Proteome Res ; 2020 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-33200940

RESUMO

Rapid early triage and dose estimation is vital for limited medical resource allocation and treatment of a large number of the wounded after radiological accidents. Lipidomics has been utilized to delineate biofluid lipid signatures after irradiation. Here, high-coverage targeted lipidomics was employed to screen radiosensitive lipids after 0, 1, 2, 3, 5, and 8 Gy total body irradiation at 4, 24, and 72 h postirradiation in rat plasma. Ultra-performance liquid chromatography-tandem mass spectrometry with a multiple reaction monitoring method was utilized. In total, 416 individual lipids from 18 major classes were quantified and those biomarkers altered in a dose-dependent manner constituted panel A-panel D. Receiver operator characteristic curve analysis using combined lipids showed good to excellent sensitivity and specificity in triaging different radiation exposure levels (area under curve = 0.814-1.000). The equations for dose estimation were established by stepwise regression analysis for three time points. A novel strategy for radiation early triage and dose estimation was first established and validated using panels of lipids. Our study suggests that it is feasible to acquire quantitative lipid biomarker panels using targeted lipidomics platforms for rapid, high-throughput triage, which can provide further insights in developing lipidomics strategies for radiation biodosimetry in humans.

5.
Antiviral Res ; 184: 104953, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-33065138

RESUMO

BACKGROUND & AIMS: Normal/mildly elevated ALT (<2 × ULN) CHB patients are potentially at risk of progression to cirrhosis and/or hepatocellular carcinoma (HCC). We aimed to assess the outcomes of anti-viral therapy for normal/mild elevation of ALT CHB patients. METHODS: CHB patients (n = 432) who have had liver biopsied were determined. It was determined that the outcomes of anti-viral therapy in CHB patients with normal/mild elevation of ALT, in response to nucleoside/nucleotide analogues (NAs) (n = 190) and pegylated interferon (PEG-IFN) (n = 30) treatment for up to 72 weeks. Non-anti-viral treated patients were used as control (n = 40). RESULTS: There was about 50% of the CHB patients showed hepatic inflammatory necrosis ≥ G2 and/or fibrosis ≥ S2 among >30-years-old. The rate of undetectable HBV DNA in NAs and PEG-IFN groups was ~50%, ~80% or ~90% at week 24, 48 or 72, respectively. HBeAg clearance rate was lower in NAs treated than that in PEG-IFN group at week 48 (6% vs 20%, P < 0.05). ALT normalization rate was increased by 1.18-fold at week 72. HBsAg decline in HBeAg+ patients treated with NAs or PEG-IFN was 0.418 or 1.217 log IU/mL (P < 0.0001) at week 48; whereas HBsAg decline was 0.176 or 0.816 log IU/mL (P < 0.001) in HBeAg- patients. HBsAg at baseline and week 24 were strong predictors of "low HBsAg at week 48". CONCLUSION: Long term anti-viral therapy inhibits HBV replication effectively in ALT<2 × ULN CHB patients. PEG-IFN therapy is recommended for HBeAg+ patients with baseline HBsAg<4.37 log IU/ml and HBeAg- patients with baseline HBsAg<2.66 log IU/ml to achieve "low HBsAg at week 48".

6.
Front Physiol ; 11: 526, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32655398

RESUMO

Background and Aims: Necroptosis is a newly identified type of cell death with programmed pathways. The current study was performed to investigate necroptosis by measuring its key regulators; receptor interacting protein kinase 3 (RIPK3) and mixed lineage kinase domain-like (MLKL) in patients with Hepatitis B virus (HBV) related acute-on-chronic liver failure (ACLF). Methods: HBV-related ACLF (HBV-ACLF) patients (n = 90), non-ACLF patients without cirrhosis (N = 70), patients with cirrhosis (N = 40), and healthy controls (HCs; n = 70) were enrolled in the study. All patients were subject to serum RIPK3 measurement. Hepatic RIPK3 and MLKL were also determined in the livers of 18 patients and five donors, using immunohistochemistry. Results: Serum RIPK3 was significantly elevated in HBV-ACLF patients compared to that of non-ACLF patients and the HCs. Serum RIPK3 in ACLF patients at recruitment was significantly higher in non-survivors than those in survivors at the 90-day follow-up. The predictive accuracy of serum RIPK3 at the 90-day outcome was relatively good with an area under the receiver operating curve (AUROC) of 0.72 (p < 0.001), similar to that of the model of end-staged liver disease (MELD) score (0.76, p < 0.001). The combined use of RIPK3 and MELD score further increased the AUROC to 0.80. The hepatic RIPK3 and MLKL measured by immunohistochemistry, significantly increased in the patients with HBV-ACLF than in the patients without ACLF and the HCs. Conclusion: Circulating RIPK3 was significantly increased in patients with HBV-ACLF and was associated with a clinical outcome. The improved combined objective scores could offer additional prognostic value in ACLF patients, for physicians with more accurate expectations.

7.
Chemosphere ; 260: 127651, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32688324

RESUMO

Phosphate (P) fertilization is a commonly used agronomic practice. However, research on bioremediation is very limited. This study's principal objective was to evaluate the role of P in the growth and heavy metals (HMs) accumulation of Polygonum pubescens Blume cultured in Mn-contaminated soil. To this end, the effects of sodium dihydrogen phosphate (SDP) and single superphosphate (SSP) on the growth, Mn bioremediation efficiency, organ HMs, and physiological parameters related to antioxidant stress of P. pubescens were examined. The results showed that both SDP and SSP increased soil pH and available P but decreased available HMs. Phosphate significantly (P < 0.05) promoted P. pubescens height and biomass. Average height increased by 36.1% and 32.6% with SDP and SSP, respectively, with corresponding biomass increases of 71.8% and 135%. Phosphate significantly (P < 0.05) reduced Mn concentrations, especially in leaves, where the values decreased by >50.0% for DSP and SSP. Total Mn significantly (P < 0.05) decreased with DSP amendment but significantly (P < 0.05) increased by 38.5% with SSP (200 mg kg-1) through an increase in biomass. Phosphate significantly (P < 0.05) decreased all organ HM concentrations and translocation, indicating that less HM stress occurred with P amendment. The changes in reactive oxygen species, antioxidants and non-antioxidant materials further supported these results. Pearson correlation analysis revealed negative relationships between soil available P and HMs, indicating a novel role of P in HM passivation. The uncommonly high Ca concentrations in leaves suggested that Ca plays a vital role in promoting growth and alleviating HM stress in P. pubescens, which warrants further study.


Assuntos
Biodegradação Ambiental , Manganês/metabolismo , Poluentes do Solo/metabolismo , Biomassa , Manganês/análise , Metais Pesados/análise , Fosfatos/análise , Folhas de Planta/química , Polygonum/crescimento & desenvolvimento , Solo , Poluentes do Solo/análise
8.
Environ Sci Pollut Res Int ; 27(16): 19933-19945, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32232756

RESUMO

Mine tailings are a characteristic of landscapes where mineral extraction has occurred and provide a prime opportunity for vegetation succession. In this study, soil heavy metal concentrations, plant composition and biodiversity, heavy metal accumulation, and their relationships were studied in the tailings of the Pingle Mn mine (abandoned for over 15 years) in South China. The total heavy metal concentrations ranged from 440 to 15,590 mg kg-1 for Mn, 5.01 to 20.7 mg kg-1 for Cd, 101 to 319 mg kg-1 for Pb, 546 to 1693 mg kg-1 for Zn, and 116 to 180 mg kg-1 for Cu. According to soil contamination assessment by single contamination indexes and the Nemerow multifactor index, the tailing soil had a heavy pollution level. According to ecological risk assessment by monomial potential ecological risk factors and potential ecological risk indexes, the tailing soil presented a high ecological risk level, to which Cd was the key contributor. A total of 13 plant species from 2 families (Gramineae and Compositae) successfully colonized the tailings. Importance values based on relative height, relative coverage, relative abundance, and relative frequency indicated that Neyraudia reynaudiana K. and Paspalum orbiculare F. were the dominant species. The species were multi-metal-tolerant species, and most of them were shoot accumulators, as their translocation factor values were above 1. Plants exhibited the highest bioconcentration factor for Pb, and the average values for roots, stems, and leaves were 2.56, 1.45, and 1.70, respectively. There were positive relationships (P < 0.01) between soil Mn, Cd, Zn, and Cu and plant Pb; similar results were found for soil heavy metals and leaf/stem Mn. The species composition in the tailings of the Pingle Mn mine was reflective of long-term vegetation succession, and the results obtained in this study provide insight for selecting plant species and reconstruction practices for Mn wasteland restoration.


Assuntos
Metais Pesados/análise , Poluentes do Solo/análise , China , Monitoramento Ambiental , Manganês , Solo
9.
Int J Phytoremediation ; 22(11): 1156-1167, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32202138

RESUMO

The seed germination plant growth parameters and level of heavy metal accumulation were investigated in pakchoi cultured in four contaminated soils with different levels of heavy metals supplemented with citric acid (CA) or calcium phosphate (CP). Results showed that the seed germination energy, germination percentage and germination index parameters were similar, while the seed vigor (SV) significantly (p < 0.05) decreased as the soil pollution level increased. The lengths of the shoots and roots presented the same trend as SV. All the seedlings grew in heavily polluted soil without any amendments before harvesting; therefore, no plant material was available for subsequent analyses. The photosynthesis parameters of pakchoi cultured in lightly polluted soil without amendment (LPS), lightly polluted soil with CA (LPSA) and moderately polluted soil with CP (MPSP) were similar. The concentrations of Pb, Zn, Mn, Cu and Cd in the shoots, roots and whole plants were in the order of MPSP > LPSA > LPS. Pakchoi cultured in MPSP showed the most promising results in terms of plant height, biomass and heavy metal accumulation. Pakchoi presented the highest translocation and bioaccumulation factors for Cd and the lowest for Pb.HighlightsSoil pollution and the type of chemical amendment had no effect on the seed germination of pakchoi.Citric acid addition in lightly polluted soil improved pakchoi growth and heavy metal extraction.Pakchoi cultured in moderately polluted soil with calcium phosphate amendment presented the highest biomass and heavy metal concentration.


Assuntos
Metais Pesados/análise , Poluentes do Solo/análise , Biodegradação Ambiental , Germinação , Sementes , Solo
10.
Chemosphere ; 251: 126415, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32169698

RESUMO

In 1976, a tailing dam collapse accident at the Xingping Lead-Zinc Mine in Guangxi Province, China, led to the spillage of mining wastewater and sludge into downstream agricultural lands in Side Village. Heavy metal concentrations, soil pollution and soil enzyme activity were analysed to understand the pollution characteristics of the agricultural lands along the Side stream by this accident. The tailing soil (TS), natural forest soil without spill contamination (NFS) and four representative agricultural soils were selected. The four agricultural soil sites located at the entrance of the stream to the agricultural soil (EnS), the upstream region (US), the middle stream (MS) and at the exit of the stream (ExS) from the village, respectively. The results showed that the soil pH values and heavy metal concentrations were in the order of tailing soil TS > ExS > MS > US > EnS > NFS. The concentration of Pb ranked highest among the analysed elements followed by Zn, Cd, Mn and Cu. The concentrations of Pb, Zn, Cd Mn and Cu 10530.41, 1708.58, 8.32, 885.61 and 104.51 mg kg-1, respectively. Soil pollution assessments by single pollution index, synthesis pollution index and individual/comprehensive potential ecological risk analysis explicated all the soils reached the heavily polluted level and presented extremely high ecological risk grades. Pb, Zn and Cd were the dominant pollutants. The soil enzyme activities of invertase, protease and urease exhibited the opposite distribution pattern as those of the heavy metal concentrations, while the inversely results were observed for the activity of catalase.


Assuntos
Monitoramento Ambiental , Metais Pesados/análise , Poluentes do Solo/análise , Agricultura , China , Poluição Ambiental/análise , Chumbo/análise , Mineração , Medição de Risco , Solo/química , Zinco/análise
11.
Cancer Med ; 9(9): 3057-3069, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32150664

RESUMO

BACKGROUND: Alpha-fetoprotein (AFP), routinely used for diagnosis of hepatocellular carcinoma (HCC), is limited with relatively low sensitivity and high false positivity in HBV-related HCC (HBV-HCC). Thus, an alternative approach was explored to improve specificity/sensitivity for diagnosis of HBV-HCC, using the combination of AFP, inflammatory score, and liver function. METHODS: Chronic hepatitis B (CHB) (n = 510) and HBV-HCC (n = 473) patients were identified retrospectively for this study. The diagnostic value of single vs combined biomarkers for HBV-HCC was analyzed, using ROC curve. RESULTS: It was observed that elderliness, male sex, cirrhosis, HBeAg+ or no-antiviral therapy, and elevation of ALT, AST, neutrophil-lymphocyte ratio (NLR), and AFP were associated with developing HBV-HCC. However, the cut-off ALT defined by Chinese standard, but not by AASLD, was a risk factor. Interestingly, AFP of HBeAg- HBV-HCC patients without cirrhosis was significantly higher than that of the HBeAg+ patients. AUC values for AFP, ALT, AST, or NLR were 0.84 (95% CI: 0.815-0.862), 0.533 (95% CI: 0.501-0.565), 0.696 (95% CI: 0.666-0.725), or 0.684 (95% CI: 0.654-0.713) with optimal cut-off at 7.21 ng/mL, 43 IU/mL, 38 IU/mL, or 2.61, respectively. Combination of AFP with ALT, AST, and NLR improved the diagnostic performance for HBV-HCC, compared to any of the single biomarkers or any other combinations among these patients (except no-cirrhosis). CONCLUSIONS: Elderliness, male sex, elevated ALT, AST, NLR, AFP, cirrhosis, HBeAg+ , and no-antiviral treatment were independent risk factors for HBV-HCC. AASLD standard of ALT cut-off value may not be suitable for the Chinese population. Regular monitoring of HCC among HBeAg- patients with abnormal AFP may improve the management of HBV-HCC. The diagnostic performance of AFP combined with ALT, AST, and NLR for HBV-HCC was superior to single biomarker or any other combinations among these patients, and its diagnostic equation can be used as useful tool for differentiation of HBV-HCC from CHB.

12.
Int J Phytoremediation ; 22(10): 1075-1084, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32064892

RESUMO

The effects of potassium (K) fertilization (KCl, analytically pure; 0, 60, 200, and 400 mg kg-1) on the growth and Mn accumulation of Camellia oleifera in two types of Mn-contaminated soils were investigated. The potential mechanisms underlying the impacts of K fertilization were explored. C. oleifera accumulated high amounts of Mn in both soil conditions. The addition of K fertilizer decreased the soil pH and promoted Mn accumulation in C. oleifera. However, the plant biomass decreased significantly under the high level of K fertilization (400 mg kg-1), and the oxidative stress was stimulated under Mn contamination. But an appropriate concentration of K fertilizer (200 mg kg-1) was necessary for the formation of photosynthesis pigments, nonenzymatic antioxidants and antioxidant enzymes, metabolic processes, and nutrient uptake. Furthermore, when plants supplemented with a low level of K fertilization (200 mg kg-1), the catalase activity in C. oleifera leaves was enhanced to alleviate oxidative stress and protect the plant from Mn contamination. Our study demonstrated that 200 mg kg-1 of K fertilizer has the potential to further enhance the efficiency of Mn phytoremediation by C. oleifera.


Assuntos
Camellia , Poluentes do Solo/análise , Biodegradação Ambiental , Manganês , Potássio , Solo
13.
Ecotoxicol Environ Saf ; 184: 109603, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31473561

RESUMO

Manganese (Mn) pollution in soil, especially around the mining areas, is a severe problem in China. Seeking for effective remediation methods for Mn-contaminated soil is therefore urgent and necessary. Camellia oleifera (C. oleifera) is one of the world's four major woody oil plants, which is widely cultivated in subtropical acidic soils for oil production and has become an important economic and ecological resource in Guangxi Province. Nitrogen (N) is one of the most common limiting factors for plant growth and development in soils. We carried out this study to evaluate the effects of different N fertilization levels (0, 100, 300 and 500 mg kg-1) on the morphological and physiological characteristics of C. oleifera in two soils with different Mn-contamination degrees. The results indicate that N fertilization affected the plant growth and the content of photosynthetic pigments, while C. oleifera accumulated great amounts of Mn in both soils. However, the plant biomass reduced significantly at the high-level N fertilization (≥300 mg kg-1), and the oxidative stress was stimulated under Mn contamination. As a comparison, the plant biomass remained unaffected at the low-level N fertilization (100 mg kg-1), and the ascorbate peroxidase (APX) activity in C. oleifera leaves were enhanced to alleviate the oxidative stress and therefore protecting the plant from Mn contamination. Meanwhile, plants supplemented with a low-level of N fertilizer (100 mg kg-1) had appropriate antioxidant enzyme and nonenzymatic antioxidant activities, which indicates that this was favorable growth conditions for C. oleifera. Thus, the recommended N fertilization level for maintaining plant biomass and increasing Mn accumulation in plant is 100 mg kg-1 N; at which level the efficiency of Mn phytoremediation by C. oleifera can be further enhanced.


Assuntos
Camellia/efeitos dos fármacos , Fertilizantes , Manganês/metabolismo , Nitrogênio/farmacologia , Poluentes do Solo/metabolismo , Biodegradação Ambiental , Biomassa , Camellia/crescimento & desenvolvimento , Camellia/metabolismo , Camellia/fisiologia , China , Fertilizantes/análise , Nitrogênio/análise , Estresse Oxidativo , Solo/química
14.
Int J Phytoremediation ; 21(12): 1225-1233, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31140289

RESUMO

This study examined how different nitrogen (N) forms and application levels promote plant growth and assist in manganese (Mn) remediation of Polygonum pubescens Blume (P. pubescens) cultured in soil with a high Mn level. The effects of ammonium chloride (a) and urea (u), at three application levels (10, 20, and 30 mg L-1 N) and control (no N addition, CK) on the growth, Mn accumulation, and enzymatic anti-oxidative defenses of P. pubescens were examined. In general, both ammonium-N and urea-N promoted the plant mass and height of P. pubescens. The total Mn amount of roots, stems, and leaves in N treatments were higher (p < 0.05) than that of CK. The ammonium-N treatments showed greater plant biomass and Mn accumulation compared to the urea-N ones. In general, the accumulations of Mn, Cr, Zn, and Cu were significantly lower (p < 0.05) in the N fertilizer treatment than those in the control; while the accumulations of Pb were higher (p < 0.05) in P. pubescens across all N fertilizer treatments than those in the control. The N addition decreased the contents of O2- and H2O2 in the leaves of P. pubescens, while increasing the activities of enzymatic anti-oxidative defenses.


Assuntos
Polygonum , Poluentes do Solo , Biodegradação Ambiental , Fertilizantes , Peróxido de Hidrogênio , Manganês , Nitrogênio , Solo
15.
Environ Sci Pollut Res Int ; 26(21): 21329-21339, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31124060

RESUMO

This study intended to help illustrate the Mn accumulation ability of Camellia oleifera and provide it as a novel species for possible use in Mn-contaminated sites. Field surveys have been carried out on Mn accumulation in C. oleifera growing near Mn mining area in Hezhou. Pot growth experiments in soil and sand culture were conducted to investigate Mn tolerance, accumulation, and translocation patterns in C. oleifera. C. oleifera grew well and showed no symptoms of Mn toxicity at a Mn treatment level below 1026 mg kg-1 in soil culture and 15.0 mmol L-1 in sand culture. Mn concentrations in leaves and stems reached a maximum of 9612.8 ± 83.5 and 6134.8 ± 94.0 mg kg-1, respectively, in soil culture and 28,465.8 ± 1276.7 and 15,398.4 ± 1148.6 mg kg-1, respectively, in sand culture. Meanwhile, most of the Mn taken from the substrates was transported to the aboveground tissues in soil and sand culture, e.g., over 92.07% of the total Mn taken up by C. oleifera was translocated to shoots in the 10.0 mmol L-1 treatment. Our findings confirmed that C. oleifera exhibited extraordinary Mn accumulation and toleration abilities, and C. oleifera was a suitable species for phytoremediation of Mn-contaminated sites in Guangxi Province.


Assuntos
Biodegradação Ambiental , Camellia/fisiologia , Manganês/toxicidade , Poluentes do Solo/toxicidade , Adaptação Fisiológica , China , Manganês/análise , Mineração , Solo , Poluentes do Solo/análise , Madeira/química
16.
Biosci Trends ; 13(2): 130-135, 2019 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-30930359

RESUMO

Single nucleotide polymorphisms (SNP) influence the outcome of antiviral therapy in chronic hepatitis B patients. Interferon ß promoter stimulator 1 polymorphisms (IPS-1) regulate interferon (IFN) mediated viral clearance in hepatitis B virus (HBV) infection. In our study, HepG2 and HepG2.2.15 were transfected with different SNP genotype expression vectors of IPS-1 (wild-type, rs17857295, rs7262903 and rs7269320). The production of IPS-1 and IFN were evaluated in these transfected cells. IPS-1 in the HepG2.2.15 cells transfected with rs17857295 or rs7262903 was 37% or 31% lower than that with wild-type transfection (p < 0.001). IFN-ß in rs17857295 or rs7262903 transfected HepG2.2.15 cells was 5.4 or 3.7 fold higher than that of wild-type transfection (p < 0.0001). IPS-1 in rs7269320 SNP transfected HepG2.2.15 cells was 40% lower than that of wild-type transfection (p < 0.0001); no significantly different IFN-ß was observed between rs7269320 SNP and wild-type transfections. IFN-ß expression was > 2 fold higher in rs17857295 transfected HepG2.2.15 cells than HepG2 cells (p < 0.001). The data suggests that host HBV viral clearance is stronger in IPS-1 rs17857295 or rs7262903 SNP genotype patients than wild-type patients. Relatively weak inducible IFN-ß production in HBV infected patients with IPS-1 rs7269320 SNP or wild-type may contribute to chronic virus infection.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/genética , Hepatite B/genética , Interferon beta/metabolismo , Polimorfismo de Nucleotídeo Único/genética , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Vetores Genéticos/metabolismo , Genótipo , Células HEK293 , Células Hep G2 , Humanos , RNA Mensageiro/genética , RNA Mensageiro/metabolismo
17.
J Viral Hepat ; 26(7): 835-845, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-30974482

RESUMO

Extensive hepatocyte death leads to hepatic inflammation and contributes to systemic inflammation in decompensated cirrhosis. We aimed to investigate the prognostic value of serum cell death markers in patients with hepatitis B virus (HBV)-related acute decompensation (AD) of cirrhosis with and without acute-on-chronic liver failure (ACLF). We studied two cohorts-cohort 1: 201 outpatients with stable chronic hepatitis B (49 cirrhosis); cohort 2: 232 inpatients with HBV-related cirrhosis admitted for AD. Cell death was determined with serum keratin-18 (K18) for total death and serum caspase-cleaved-K18 (cK18) for apoptosis. Survival analyses were performed using competing risk method. We found that serum K18 and cK18 were significantly (P < 0.001) higher in patients from cohort 2 than those from cohort 1. Among cohort 2, ACLF patients had significantly (P < 0.001) increased K18 and cK18 comparing to those without ACLF. Increased K18 and cK18 were mainly attributed to HBV flare and were associated with liver and coagulation failure. HBV-AD patients without ACLF who admitted with upper tertile of K18 or cK18 were at higher risk of developing ACLF during follow-up. Baseline serum K18 or cK18 was significantly associated with transplant-free 90-day survival independent of leucocytes, HBV DNA, bacterial infection, encephalopathy and severity scores. The combination of cell death biomarkers significantly improved the prognostic value of the currently established prognostic scores. The reduction of cell death level after standard treatment was associated with increased short-term survival. In conclusion, measurements of serum K18 or cK18 in HBV decompensated cirrhosis are a promising tool for predicting ACLF and risk stratification of short-term outcome.


Assuntos
Biomarcadores , Hepatite B Crônica/sangue , Queratina-18/sangue , Cirrose Hepática/sangue , Cirrose Hepática/diagnóstico , Adulto , Morte Celular , Progressão da Doença , Feminino , Seguimentos , Hepatite B Crônica/complicações , Hepatite B Crônica/diagnóstico , Hepatite B Crônica/virologia , Humanos , Cirrose Hepática/etiologia , Cirrose Hepática/mortalidade , Testes de Função Hepática , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Prospectivos , Análise de Sobrevida , Carga Viral
18.
Cancer Manag Res ; 11: 2015-2025, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30881126

RESUMO

Purpose: A better understanding of the underlying molecular mechanisms in treatment failure of bevacizumab (BEV) for malignant glioma would contribute to overcome therapeutic resistance. Methods: Here, we used a quantitative proteomic method to identify molecular signatures of glioblastoma cell after BEV treatment by two-dimensional liquid chromatography-tandem mass spectrometry analysis and 6-plex iTRAQ quantification. Next, the function of cold-inducible RNA-binding protein (CIRP), one of the most significantly affected proteins by drug treatment, was evaluated in drug resistance of glioma cells by invasion assays and animal xenograft assays. Target molecules bound by CIRP were determined using RNA-binding protein immunoprecipitation and microarray analysis. Then, these mRNAs were identified by quantitative real-time PCR. Results: Eighty-seven proteins were identified with significant fold changes. The biological functional analysis indicated that most of the proteins were involved in the process of cellular signal transduction, cell adhesion, and protein transport. The expression of CIRP greatly decreased after BEV treatment, and ectopic expression of CIRP abolished cell migration in BEV-treated glioma cells. In addition, CIRP could bind mRNA of CXCL12 and inhibit BEV-induced increase of CXCL12 in glioma cells. Conclusion: These data suggested that CIRP may take part in BEV-induced migration of gliomas by binding of migration-relative RNAs.

19.
Ecotoxicol Environ Saf ; 173: 235-242, 2019 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-30772713

RESUMO

The effects of water-extractable Mn concentration, bioaccumulation factor (BAF), translocation factor (TF), and Mn uptake by Polygonum pubescens Blume cultured in the unexplored soil, mining soil and tailing soil from the Pingle Mn mine in China were quantified in a pot experiment to determine the effects of EDTA exposure on the success of phytoremediation. The results showed that EDTA significantly (P < 0.05) increased the water-extractable Mn concentration, and soils with different amounts of artificial disturbances had different responses to EDTA exposure. Low and medium EDTA concentrations might have positive effect on plant growth of P. pubescens cultured in the unexplored soil, as indicated by comparable increases in biomass, plant height and photosynthetic pigment content, but opposite results were found with high EDTA concentrations exposure. EDTA exposure had a negative effect on the growth of P. pubescens cultured in the mining soil and tailing soil. In general, the concentration of Mn in different tissues significantly (P < 0.05) increased as the EDTA concentration increased in each soil. The efficacy of Mn remediation by P. pubescens was enhanced in all three soils, with all EDTA treatments.


Assuntos
Quelantes/farmacologia , Ácido Edético/farmacologia , Manganês/metabolismo , Polygonum/efeitos dos fármacos , Poluentes do Solo/metabolismo , Biodegradação Ambiental , Biomassa , China , Mineração , Polygonum/crescimento & desenvolvimento , Polygonum/metabolismo
20.
Infect Drug Resist ; 11: 2001-2009, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30464542

RESUMO

Background and aims: Chronic hepatitis B (CHB) patients rarely achieve hepatitis B surface antigen (HBsAg) loss with nucleoside/nucleotide analog therapy. Methods: In this retrospective study, it was evaluated that the rate of HBsAg loss in the HBe antigen negative (HBeAg-) patients (n=101) treated with entecavir (ETV) for ≥24 weeks followed by switching to (n=22) or adding on (n=26) pegylated interferon (PEG-IFN), and continuing ETV (n=53). Results: HBsAg clearance rate at week 48 was 9% (2/22), 15% (4/26), and 0% (0/53) (P<0.05), in switch-to or add-on, or ETV monotherapy CHB patients, respectively. HBsAg reduction at week 48 was 1.182, 0.6614, or 0.056 log IU/mL, in switch-to, add-on, and ETV patients, respectively (P<0.001). The response rate (HBsAg reduction >1 log IU/mL at week 48) in the switch-to, add-on, and ETV monotherapy CHB patients was 60%, 40%, and 2%, respectively (P<0.001). In the switch-to and add-on patients, HBsAg reduction and clearance were associated with HBsAg titers at week 0 and HBsAg reduction at week 24. Furthermore, HBsAg reduction at week 24 was associated with the response rate at week 48 in the switch-to and add-on patients, showing that the area under the receiver operating characteristic curve was 0.904. Positive predictive value and negative predictive value for response rate was 70% and 100% with cut-off value 0.2 log IU/mL, respectively. Conclusion: In summary, we demonstrated that PEG-IFN enhanced HBsAg loss in HBeAg- CHB patients. High HBsAg clearance was achieved in the patients with HBsAg titers at baseline <1,000 IU/mL and HBsAg reduction >0.2 log IU/mL.

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