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1.
J Clin Invest ; 130(3): 1252-1270, 2020 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-32039918

RESUMO

Current antiangiogenic therapy is limited by its cytostatic property, scarce drug delivery to the tumor, and side toxicity. To address these limitations, we unveiled the role of ZEB1, a tumor endothelium-enriched zinc-finger transcription factor, during tumor progression. We discovered that the patients who had lung adenocarcinomas with high ZEB1 expression in tumor endothelium had increased prevalence of metastases and markedly reduced overall survival after the diagnosis of lung cancer. Endothelial ZEB1 deletion in tumor-bearing mice diminished tumor angiogenesis while eliciting persistent tumor vascular normalization by epigenetically repressing TGF-ß signaling. This consequently led to improved blood and oxygen perfusion, enhanced chemotherapy delivery and immune effector cell infiltration, and reduced tumor growth and metastasis. Moreover, targeting vascular ZEB1 remarkably potentiated the anticancer activity of nontoxic low-dose cisplatin. Treatment with low-dose anti-programmed cell death protein 1 (anti-PD-1) antibody elicited tumor regression and markedly extended survival in ZEB1-deleted mice, conferring long-term protective anticancer immunity. Collectively, we demonstrated that inactivation of endothelial ZEB1 may offer alternative opportunities for cancer therapy with minimal side effects. Targeting endothelium-derived ZEB1 in combination with conventional chemotherapy or immune checkpoint blockade therapy may yield a potent and superior anticancer effect.

2.
Toxicol Appl Pharmacol ; 389: 114882, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31953203

RESUMO

Pulmonary fibrosis is a prototypic chronic progressive lung disease with high morbidity and mortality worldwide. Novel effective therapeutic agents are urgently needed owing to the limited treatment options in clinic. Herein, nagilactone D (NLD), a natural dinorditerpenoid obtained from Podocarpus nagi, was found to suppress transforming growth factor-ß1 (TGF-ß1)-mediated fibrotic process in vitro and bleomycin (BLM)-induced pulmonary fibrosis in vivo. NLD attenuated TGF-ß1-induced expression of fibrotic markers including type I and III collagen, fibronectin, α-SMA, and CTGF in human pulmonary fibroblasts (WI-38 VA-13 and HLF-1 cells). Mechanism study indicated that NLD suppressed TGF-ß1-induced up-regulation of TßR I, and Smad2 phosphorylation, nuclear translocation, and transcriptional activation. Moreover, NLD ameliorated BLM-induced histopathological abnormalities in the lungs of experimental fibrotic mice, suppressed synthesis of relative fibrotic markers and fibroblast-to-myofibroblast transition, as well as BLM-induced up-regulation of TßR I expression and Smad signaling in mouse lungs. These data collectively support NLD to be a potential therapeutic agent for pulmonary fibrosis.

3.
Angew Chem Int Ed Engl ; 58(36): 12705-12710, 2019 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-31297923

RESUMO

Exploring dynamic bonds and their applications in fabricating dynamic materials has received great attention. A photoinduced [2]rotaxane-based dynamic mechanical bond (DMB) features visible-light-triggered dynamic bonding behavior that is essentially distinguished from conventional dynamic chemical bonds. In this DMB, a photoisomerizable ortho-fluoroazobenzene unit is introduced as a steric-controllable stopper, the visible-light-induced dynamic wagging movement of which enables the photoregulated threading of the macrocycle. This allows reversible in situ de-/reforming of the mechanical bond without involving dynamic chemical linkage. The DMB-cross-linked polymeric gel shows interesting photoinduced degradation behavior upon visible light irradiation. Benefiting from the distinctive dual dynamic nature of reversible bonding behavior and mechanical interlocked structure, this DMB is expected to serve as a new type of dynamic bond that can be applied in designing dynamic soft materials.

4.
Nat Commun ; 10(1): 3210, 2019 07 19.
Artigo em Inglês | MEDLINE | ID: mdl-31324807

RESUMO

Accumulating evidence indicates that the zinc-finger transcription factor ZEB1 is predominantly expressed in the stroma of several tumours. However, the role of stromal ZEB1 in tumour progression remains unexplored. In this study, while interrogating human databases, we uncover a remarkable decrease in relapse-free survival of breast cancer patients expressing high ZEB1 levels in the stroma. Using a mouse model of breast cancer, we show that ZEB1 inactivation in stromal fibroblasts suppresses tumour initiation, progression and metastasis. We associate this with reduced extracellular matrix remodeling, immune cell infiltration and decreased angiogenesis. ZEB1 deletion in stromal fibroblasts increases acetylation, expression and recruitment of p53 to FGF2/7, VEGF and IL6 promoters, thereby reducing their production and secretion into the surrounding stroma. Importantly, p53 ablation in ZEB1 stroma-deleted mammary tumours sufficiently recovers the impaired cancer growth and progression. Our findings identify the ZEB1/p53 axis as a stroma-specific signaling pathway that promotes mammary epithelial tumours.


Assuntos
Fibroblastos/metabolismo , Neoplasias Epiteliais e Glandulares/metabolismo , Transdução de Sinais , Proteína Supressora de Tumor p53/metabolismo , Homeobox 1 de Ligação a E-box em Dedo de Zinco/metabolismo , Animais , Mama/patologia , Neoplasias da Mama/metabolismo , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Proliferação de Células , Transformação Celular Neoplásica/metabolismo , Matriz Extracelular/metabolismo , Feminino , Fator 2 de Crescimento de Fibroblastos/metabolismo , Fator 7 de Crescimento de Fibroblastos/metabolismo , Deleção de Genes , Regulação Neoplásica da Expressão Gênica , Predisposição Genética para Doença/genética , Humanos , Interleucina-6 , Masculino , Neoplasias Mamárias Experimentais/metabolismo , Neoplasias Mamárias Experimentais/patologia , Camundongos , Camundongos Knockout , Recidiva Local de Neoplasia/metabolismo , Neoplasias Experimentais , Neoplasias Epiteliais e Glandulares/patologia , Microambiente Tumoral , Fator A de Crescimento do Endotélio Vascular/metabolismo , Homeobox 1 de Ligação a E-box em Dedo de Zinco/genética
5.
MycoKeys ; (46): 119-150, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30814907

RESUMO

Neostagonosporellasichuanensis sp. nov. was found on Phyllostachysheteroclada collected from Sichuan Province in China and is introduced in a new genus Neostagonosporella gen. nov. in this paper. Evidence for the placement of the new taxon in the family Phaeosphaeriaceae is supported by morphology and phylogenetic analysis of a combined LSU, SSU, ITS and TEF 1-α DNA sequence dataset. Maximum-likelihood, maximum-parsimony and Bayesian inference phylogenetic analyses support Neostagonosporella as a distinct genus within this family. The new genus is compared with related genera of Phaeosphaeriaceae and full descriptions and illustrations are provided. Neostagonosporella is characterised by its unique suite of characters, such as multiloculate ascostromata and cylindrical to fusiform, transversely multiseptate, straight or curved ascospores, which are widest at the central cells. Conidiostromata are multiloculate, fusiform to long fusiform or rhomboid, with two types conidia; macroconidia vermiform or subcylindrical to cylindrical, transversely multiseptate, sometimes curved, almost equidistant between septa and microconidia oval, ellipsoidal or long ellipsoidal, aseptate, rounded at both ends. An updated phylogeny of the Phaeosphaeriaceae based on multigene analysis is provided.

6.
J Exp Clin Cancer Res ; 38(1): 134, 2019 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-30898152

RESUMO

BACKGROUND: The transforming growth factor ß (TGFß) and bone morphogenetic protein (BMP) signaling pathways are both constitutively activated in triple-negative breast cancer (TNBC). We are interested in isolating the naturally-derived small-molecule inhibitor that could simultaneously targeting TGFß/BMP pathways and further studying its anti-proliferative/-metastatic effects as well as the underlying mechanisms in multiple tumor models. METHODS: Multiple in vitro cell-based assays are used to examine the compound's inhibitory efficacy on TNBC cell growth, stemness, epithelial-mesenchymal transition (EMT), invasion and migration by targeting TGFß/BMP signaling pathways. Transgenic breast cancer mouse model (MMTV-PyMT), subcutaneous xenograft and bone metastasis models are used to examine ZL170's effects on TNBC growth and metastasis potentials in vivo. RESULTS: ZL170 dose-dependently inhibits cell proliferation, EMT, stemness, invasion and migration in vitro via specifically targeting canonical TGFß/BMP-SMADs pathways in TNBC cells. The compound significantly hinders osteolytic bone metastasis and xenograft tumor growth without inflicting toxicity on vital organs of tumor-bearing nude mice. ZL170 strongly inhibits primary tumor growth and lung metastases in MMTV-PyMT transgenic mice. ZL170-treated tumors exhibit impaired TGFß/BMP signaling pathways in both epithelial and stromal compartments, thereby creating a suppressive tumor microenvironment characterized by reduced extracellular matrix deposition and decreased infiltration of stromal cells. CONCLUSIONS: ZL170 inhibits tumor EMT, stemness and metastasis and could be further developed as a potent anti-metastatic agent used in combination with cytotoxic drugs for treatment of TNBC and other advanced metastatic cancers.


Assuntos
Transição Epitelial-Mesenquimal/efeitos dos fármacos , Células-Tronco Neoplásicas/efeitos dos fármacos , Oxindois/administração & dosagem , Transdução de Sinais/efeitos dos fármacos , Bibliotecas de Moléculas Pequenas/administração & dosagem , Neoplasias de Mama Triplo Negativas/tratamento farmacológico , Animais , Proteínas Morfogenéticas Ósseas/metabolismo , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular , Feminino , Humanos , Camundongos , Camundongos Nus , Camundongos Transgênicos , Células-Tronco Neoplásicas/metabolismo , Oxindois/farmacologia , Bibliotecas de Moléculas Pequenas/farmacologia , Fator de Crescimento Transformador beta/metabolismo , Neoplasias de Mama Triplo Negativas/genética , Neoplasias de Mama Triplo Negativas/metabolismo , Ensaios Antitumorais Modelo de Xenoenxerto
7.
Iran J Public Health ; 48(1): 112-119, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30847318

RESUMO

Background: This study aimed to evaluate the characteristics of faculty and research activities of basic stem cell research groups in China. Methods: A questionnaire was administered to persons who knew the information among 46 basic stem cell research groups in China. Multiple linear regression models and repeated-measures analyses of variance were used. Repeated-measures analyses of variance were used. Results: Of the 46 groups, 39.1% did not have any faculty recruited from abroad from 2009 to 2013, 37.0% did not have any faculty with junior-level title, 34.8% had ≤25.0% faculty with either M.D. or Ph.D. degree. Papers published in SCI journals per faculty and having faculty recruited from abroad were positively associated with research funding per faculty. The groups with faculty recruited from abroad had significantly higher research funding per faculty over time compared with the group without faculty recruited from abroad. Repeated-measures analyses of variance showed the group with faculty recruited from abroad had significantly higher research funding per faculty over time compared with the group without faculty recruited from abroad. Conclusion: To increase the development of basic stem cell research, some characteristics of human resources should be improved, and the groups should recruit more faculty with overseas experience.

8.
Phys Chem Chem Phys ; 21(7): 3795-3804, 2019 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-30714064

RESUMO

The selective removal of oxygen from 5-hydroxymethylfurfural (HMF) is challenging for the effective utilization of biomass. The catalytic mechanisms of palladium acetate toward the conversion of HMF to furfuryl alcohol (FFA), 5-methylfurfural (5-MF) and 2,5-dihydroxymethyl furan (DHMF) have been theoretically investigated. The decarbonylation of HMF to FFA includes (i) migratory extrusion, (ii) metal-acetate-co-assisted deprotonation, (iii) decarbonylation, (iv) metal-assisted deprotonation, and (v) migratory extrusion and catalyst regeneration. Both hydrogenation and deoxidation of HMF with HCOOH as the H-source involve (i) migratory extrusion, (ii) oxidative addition, (iii) reductive elimination, (iv) metal-assisted deprotonation, and (v) migratory extrusion and catalyst regeneration. The C-H bond cleavage is the crucial reaction step, in which the metal-acetate-co-assisted deprotonation is kinetically more preferable than the oxidative addition. Both FFA and DHMF are kinetically superior to 5-MF. In terms of selectivity, increasing the temperature is beneficial to decarbonylation and decreasing the temperature is advantageous to hydrogenation. The present finding provides molecular-level insight into the functions of both the metal-center and coordinated-ligand in the Pd(OAc)2 catalyst, which may drive the novel design of catalytic systems toward both decarbonylation and hydrogenation reactions.

9.
Int Heart J ; 60(2): 296-302, 2019 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-30799382

RESUMO

Recent reports show that an early repolarization pattern (ERP) is associated with a higher incidence of sudden cardiac death in patients with obstructive coronary artery disease (CAD). Sporadic case studies have pointed out that ERP might be related to obstructive CAD.In consecutive patients who had undergone coronary angiography, we investigated the relationship between ERP and obstructive CAD by evaluating its association with coronary artery stenosis.The study population consisted of 3785 patients (59.9% men; mean age 63.1 years) with or without obstructive CAD. Adjusting for major cardiovascular risk factors, ERP was significantly associated with obstructive CAD (adjusted odds ratio (OR): 2.24 [95% CI 1.70-2.95]) with an incremental predictive value (ROC AUC 0.76 versus 0.71, P = 0.02; NRI 55.3%, P < 0.001; IDI = 0.05, P = 0.008), specifically in subjects with low risk and intermediate risk. ERP also significantly improved the predictive value for multi-vessel disease (AUC: 0.77 versus 0.72, P = 0.02 for two-vessel disease; 0.79 versus 0.73, P = 0.04 for three-vessel disease). ERP was consistently associated with stenoses of 3 main coronary arteries.ERP is associated with significant increased risk for obstructive CAD.Further studies are warranted to confirm our results and to elucidate the specific pathogenic mechanisms.


Assuntos
Angiografia Coronária/métodos , Doença da Artéria Coronariana , Estenose Coronária , Vasos Coronários , Morte Súbita Cardíaca , Eletrocardiografia , China/epidemiologia , Angiografia Coronária/estatística & dados numéricos , Doença da Artéria Coronariana/complicações , Doença da Artéria Coronariana/diagnóstico , Doença da Artéria Coronariana/epidemiologia , Doença da Artéria Coronariana/fisiopatologia , Estenose Coronária/diagnóstico por imagem , Estenose Coronária/fisiopatologia , Vasos Coronários/diagnóstico por imagem , Vasos Coronários/fisiopatologia , Morte Súbita Cardíaca/epidemiologia , Morte Súbita Cardíaca/etiologia , Morte Súbita Cardíaca/prevenção & controle , Eletrocardiografia/métodos , Eletrocardiografia/estatística & dados numéricos , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Estudos Retrospectivos , Medição de Risco/métodos , Fatores de Risco , Índice de Gravidade de Doença
10.
Int J Syst Evol Microbiol ; 69(3): 811-815, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30688630

RESUMO

A facultatively anaerobic and Gram-negative bacterium, designated strain PLHSC7-2T, was isolated from the gut of sea cucumber Apostichopusjaponicus that had been collected from the coastal area of Yantai, China. The cells were rod-shaped and motile by means of polar flagella. The novel isolate grew optimally at 28-30 °C, in the presence of 2.0-3.0 % (w/v) NaCl and at pH 7.0-7.5. The sole respiratory quinone was Q-8 and the major fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C17 : 0. The predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain PLHSC7-2T was phylogenetically affiliated with the genus Motilimonas, and exhibited sequence similarity of 96.2 % toMotilimonas eburnea YH6T. The DNA G+C content was 45.5 mol%. On the basis of phenotypic , phylogenetic and genetic distinctiveness, strain PLHSC7-2T (=MCCC 1K03522T=KCTC 62589T) was classified as a novel species within the genus Motilimonas, for which the name Motilimonas pumila sp. nov. is proposed.


Assuntos
Gammaproteobacteria/classificação , Filogenia , Stichopus/microbiologia , Animais , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Gammaproteobacteria/isolamento & purificação , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
11.
Cell Physiol Biochem ; 51(3): 1301-1312, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30481776

RESUMO

BACKGROUND/AIMS: Early repolarization syndrome (ERS) has been recently recognized as early repolarization pattern with idiopathic ventricular fibrillation. However, the genetic background of ERS has not been fully understood. METHODS: A Chinese family with sudden cardiac death associated with ERS was investigated. Direct sequencing of ERS susceptibility genes was performed on the proband and family members. Whole-cell patch-clamp methods were used to characterize the mutant channel expressed in HEK 293 cells. RESULTS: One missense mutation (p. K801T) was found in the hERG (KCNH2 gene) by the direct sequencing of candidate genes. Whole cell voltage clamp studies of the K801T mutation in HEK 293 cells demonstrated a 1.5-fold increase in maximum steady state current (37.2±7.3 vs 20.3±4.4 pA/pF) that occurred at a 20 mV more positive potential compared to the wild type channels. The voltage dependence of inactivation was significantly shifted in the positive voltage direction (WT -59.5±1.4 vs K801T -44.3±1.2 mV). Kinetic analysis revealed slower inactivation rates of K801T, but faster rates of activation and deactivation. The hERG channel blockers tested inhibited K801T-hERG channel in concentration response, and the potencies of these drugs can be rank-ordered as follows: quinidine> disopyramide> sotalol> flecainide. CONCLUSION: Our study indicated that the K801T mutation caused the gain of function of hERG channels that may account for the clinical phenotype of ERS. Quinidine and disopyramide could improve the function of K801T-hERG mutant channel, and may be therapeutic options for patients with the K801T hERG mutation.


Assuntos
Canal de Potássio ERG1/genética , Mutação de Sentido Incorreto , Fibrilação Ventricular/genética , Adulto , Morte Súbita Cardíaca/etiologia , Células HEK293 , Heterozigoto , Humanos , Masculino , Linhagem , Fibrilação Ventricular/complicações
12.
Opt Lett ; 43(22): 5599-5602, 2018 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-30439905

RESUMO

We demonstrate a 266 nm ultraviolet (UV) picosecond laser by fourth-harmonic generation of a Nd:YAG laser with a 5.4 mm thick NaSr3Be3B3O9F4 (NSBBF) crystal. A maximum output power exceeding 1 W at 266 nm was obtained (the highest output power being 1.6 W), corresponding to a conversion efficiency of 10.3%. The stability measurements on the NSBBF crystal with a fluctuation of 3.34% at 200 mW within 1 h indicate that it is a promising UV nonlinear optical material for practical applications. In addition, for the first time, to the best of our knowledge, we measured the effective nonlinear coefficient of NSBBF crystal at 266 nm and compared it with that of ß-BaB2O4 crystal.

13.
Chem Commun (Camb) ; 54(67): 9356-9359, 2018 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-30079425

RESUMO

Photochromic [2]rotaxanes with bidirectional photoswitchability were fabricated, whose colored states exhibit remarkable visible-light and thermal stabilities as revealed by systematically spectroscopic investigations.

14.
J Cell Mol Med ; 22(11): 5639-5647, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30160358

RESUMO

BACKGROUND: Abnormal cardiac ion channels current, including transient outward potassium current (Ito ), is associated with early repolarization syndrome (ERS). Previous studies showed that mutations in SCN1Bß both to increase the Ito current and to decrease the sodium current. Yet its role in ERS remains unknown. OBJECTIVE: To determine the role of mutations in the SCN1Bß subunits in ERS. METHODS: We screened for mutations in the SCN1B genes from four families with ERS. Wild-type and mutant SCN1Bß genes were co-expressed with wild-type KCND3 in human embryonic kidney cells (HEK293). Whole-cell patch-clamp technique and co-immunoprecipitation were used to study the electrophysiological properties and explore the underlying mechanisms. RESULTS: S248R and R250T mutations in SCN1Bß were detected in 4 families' probands. Neither S248R nor R250T mutation had significant influence on the sodium channel current density (IN a ) when co-expressed with SCN5A/WT. Co-expression of KCND3/WT and SCN1Bß/S248R or SCN1Bß/R250T increased the transient outward potassium current Ito by 27.44% and 199.89%, respectively (P < 0.05 and P < 0.01, respectively) when compared with SCN1Bß/WT. Electrophysiological properties showed that S248R and R250T mutations decreased the steady-state inactivation and recovery from inactivation of Ito channel. Co-immunoprecipitation study demonstrated an increased association between SCN1Bß mutations and Kv4.3 compared with SCN1Bß/WT (P < 0.05 and P < 0.01, respectively). CONCLUSION: The S248R and R250T mutations of SCN1Bß gene caused gain-of-function of Ito by associated with Kv4.3, which maybe underlie the ERS phenotype of the probands.


Assuntos
Morte Súbita Cardíaca/patologia , Coração/fisiopatologia , Canais de Potássio Shal/genética , Subunidade beta-1 do Canal de Sódio Disparado por Voltagem/genética , Adulto , Idoso , Animais , Eletrofisiologia , Feminino , Predisposição Genética para Doença , Células HEK293 , Coração/diagnóstico por imagem , Humanos , Masculino , Potenciais da Membrana , Mutação/genética , Técnicas de Patch-Clamp , Transfecção
15.
Chem Asian J ; 13(19): 2818-2823, 2018 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-29975456

RESUMO

The development of artificial self-assembling systems with dynamic photo-regulation features in aqueous solutions has drawn great attention owing to the potential applications in fabricating elaborate biological materials. Here we demonstrate the fabrication of water-soluble cucurbit[8]uril (CB[8])-mediated supramolecular polymers by connecting the fluorinated azobenzene (FAB) containing monomers through host-enhanced heteroternary π-π stacking interactions. Benefiting from the unique visible-light-induced E→Z photoisomerization of the FAB photochromophores, the encapsulation behaviors between the CB[8] macrocycle and the monomers could be regulated upon visible light irradiation, resulting in the depolymerization of such CB[8]-mediated supramolecular polymers.

16.
Lancet Public Health ; 3(8): e385-e394, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-30033200

RESUMO

BACKGROUND: Transmission of infection through international travel is a growing health issue, and the frequency of imported infection is increasing in China. We aimed to quantify the total number of infections imported into mainland China by arriving travellers. METHODS: We actively surveyed arriving travellers at all 272 international entry-exit ports in mainland China. Suspected cases were detected through fever screening, medical inspection, self-declaration, and reporting by on-board staff. Participants completed a standardised questionnaire with questions about demographics, their travel itinerary (including detailed information about all countries or regions visited), and clinical manifestations. Nasopharyngeal swabs, sputum samples, faecal samples, vomitus, blood, and serum were collected as appropriate for diagnoses. Diagnosis was made by specific laboratory tests according to the national technical guidelines. Infections were classified as respiratory, gastrointestinal, vector-borne, blood-transmitted and sex-transmitted, or mucocutaneous. We divided arriving travellers into two groups: travellers coming from countries other than China, and travellers coming from Hong Kong, Macau, and Taiwan. We integrated surveillance data for 2014-16, calculated incidences of travel-related infections, and compared the frequency of infections among subgroups. FINDINGS: Between Jan 1, 2014, and Dec 31, 2016, 22 797 cases were identified among 805 993 392 arriving travellers-an overall incidence of 28·3 per million. 45 pathogens were detected in participants: 18 respiratory (19 662 cases), ten gastrointestinal (189 cases), seven vector-borne (831 cases), seven blood-transmitted and sex-transmitted (1531 cases), and three mucocutaneous (584 cases). Both the overall number and incidence of infection were more than five times higher in 2016 than in 2014. Case numbers and incidences also varied substantially by province, autonomous region, and municipality. Overall, 17 643 (77%) infections were detected by fever screening, but 753 (49%) blood-transmitted and sex-transmitted infections were identified through medical inspection. 14 305 (73%) cases of respiratory infection and 96 (51%) of gastrointestinal infections were in tourists. Tuberculosis, hepatitis A virus, vector-borne, and blood-transmitted and sex-transmitted infections were common among Chinese labourers who worked abroad. Dengue and malaria were most commonly diagnosed in travellers arriving from Africa. 12 126 (93%) of the 12 985 cases arriving from Hong Kong, Macau, or Taiwan were respiratory infections. Hand, foot, and mouth disease accounted for 2·90% of infections in travellers from Hong Kong, Macau, or Taiwan and 0·31% of infections in international travellers. INTERPRETATION: This report is the first to characterise the profile of travel-related infections among arriving travellers in mainland China. Our findings should increase public awareness of the potential risk of imported infections, and help health-care providers to make evidence-based health recommendations to travellers. FUNDING: The Natural Science Foundation of China.


Assuntos
Vigilância da População , Doença Relacionada a Viagens , Adolescente , Adulto , Criança , China/epidemiologia , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Adulto Jovem
17.
Med Sci Monit ; 24: 4305-4316, 2018 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-29934492

RESUMO

BACKGROUND The method of multiple targets overall control is increasingly used to predict the main active ingredient and potential target group of Chinese traditional medicines and to determine the mechanisms involved in their curative effects. Qingdai is the main traditional Chinese medicine used in the treatment of chronic myelogenous leukemia (CML), but the complex active ingredients and antitumor targets in treatment of CML have not been clearly defined in previous studies. MATERIAL AND METHODS We constructed a protein-protein interaction network diagram of CML with 638 nodes (proteins) and 1830 edges, based on the biological function of chronic myelocytic leukemia by use of Cytoscape, and we determined 19 key gene nodes in the CML molecule by network topological properties analysis in a data bank. Then, we used the Surflex-dock plugin in SYBYL7.3 docking and acquired the protein crystal structures of key genes involved in CML from the chemical composition of the traditional Chinese medicine Qingdai with key proteins in CML networks. RESULTS According to the score and the spatial structure, the pharmacodynamically active ingredients of Qingdai are Isdirubin, Isoindigo, N-phenyl-2-naphthylamine, and Isatin, among which Isdirubin is the most important. We further screened the most effective activity key protein structures of CML to find the best pharmacodynamically active ingredients of Qingdai, according to the binding interactions of the inhibitors at the catalytic site performed in best docking combinations. CONCLUSIONS The results suggest that Isdirubin plays a role in resistance to CML by altering the expressions of PIK3CA, MYC, JAK2, and TP53 target proteins. Network pharmacology and molecular docking technology can be used to search for possible reactive molecules in traditional chinese medicines (TCM) and to elucidate their molecular mechanisms.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Leucemia Mielogênica Crônica BCR-ABL Positiva/tratamento farmacológico , Medicina Tradicional Chinesa , Simulação de Acoplamento Molecular , Biologia de Sistemas/métodos , Avaliação Pré-Clínica de Medicamentos , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Regulação Leucêmica da Expressão Gênica/efeitos dos fármacos , Humanos , Leucemia Mielogênica Crônica BCR-ABL Positiva/genética , Ligantes , Proteínas de Neoplasias/metabolismo , Mapas de Interação de Proteínas
18.
Opt Lett ; 43(11): 2563-2566, 2018 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-29856430

RESUMO

We report a high-energy single-frequency deep-ultraviolet (DUV) solid-state laser at 167.079 nm by the eighth-harmonic generation of a diode-pumped Nd:LGGG laser. A maximum DUV laser output energy of 1.5 µJ at a 5 Hz repetition rate with a 200 µs pulse duration is achieved. The central wavelength of the DUV laser is located at 167.079 nm and can be finely tuned from 167.075 to 167.083 nm. The linewidth is estimated to be 0.025 pm. To the best of our knowledge, this is the first Letter reporting a high-energy single-frequency solid-state DUV laser below 170 nm. The successful demonstration of the high-energy single-frequency DUV laser source with the unique wavelength is useful for direct detection of a Al+27 ion via resonance fluorescence in a multi-ion optical clock.

19.
Ying Yong Sheng Tai Xue Bao ; 29(4): 1321-1327, 2018 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-29726243

RESUMO

The application of municipal sludge in ecological restoration has a good prospect for avoiding the food chain of grain crops, but its influences on surface water environmental are unclear. The municipal sludge and construction waste were mixed with 1:1 (V/V) as growth media, which were covered over simulation coal gangue slopes. Eight native woody species were sowed in the mixed media. The plant growth and coverage, as well as conductivity, pH, the concentrations of nitrogen (N), phosphorus (P), potassium (K), heavy metal and polycyclic aromatic hydrocarbon (PAHs) of surface and underground runoff of the slopes in the growing season were investigated. The results showed that plants grew well on the mixed media. The average plant coverage reached 60%. The pH of the surface and underground runoff changed little and near to neutral. The conductivity, N, P, K, heavy metal and PAHs contents of the slope runoff were high. The N and P contents in the growing season were above the National Standards of Surface Water Quality (GB 3838-2002) V. The contents of heavy metal were the highest in July. The contents of As lied at the GB IV-V, whereas other heavy metal contents up to GB II-IV. With strong rain leaching in the summer as well as the absorption, degrading and fix effect of plant-soil system on chemical substrates, the conductivity and N, P, K, heavy metal and PAHs contents of the slope runoff significantly decreased. The contents of heavy metal in late stage of growing season arrived at GB 2-3. The contents of PAHs reduced by about 50%. The direct application of municipal sludge in ecological restoration of coal gangue slope were beneficial to plant growth. The plant-soil system might gradually decrease the harmful substance concentrations in the growth media. The negative influences on surface water environment mainly came from eutrophication of N and P. Generally, the environmental safety is manageable.


Assuntos
Metais Pesados/análise , Esgotos , Poluentes do Solo/análise , Carvão Mineral , Nitrogênio , Fósforo , Desenvolvimento Vegetal , Plantas , Hidrocarbonetos Policíclicos Aromáticos , Solo
20.
Invest Ophthalmol Vis Sci ; 59(3): 1609-1616, 2018 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-29625487

RESUMO

Purpose: To assess the development and enlargement of the parapapillary gamma zone in school children. Methods: This school-based prospective longitudinal study included Chinese children attending grade 1 in 2011 and returning for yearly follow-up examinations until 2016. These examinations consisted of a comprehensive ocular examination with biometry and color fundus photographs. The parents underwent a standardized interview. The parapapillary gamma zone was defined as the area with visible sclera at the temporal optic disc margin, and the optic disc itself was measured on fundus photographs. Results: The study included 294 children (mean age in 2016, 11.4 ± 0.5 years [range, 10-13 years]; mean axial length, 24.1 ± 1.1 mm [range, 21.13-27.29 mm]). In multivariate analysis, larger increases in the gamma zone area during the study period were correlated (coefficient of determination for bivariate analysis [r2], r2 = 0.69) with larger increases in the vertical-to-horizontal disc diameter ratios (P < 0.001; standardized regression coefficient beta [beta], 0.53; nonstandardized regression coefficient B [B], 4.05; 95% confidence intervals [CI], 3.37-4.73), larger axial elongation (P < 0.001; beta, 0.32; B, 0.37; 95% CI, 0.26-0.47), a larger vertical disc diameter at baseline (P < 0.001; beta, 0.22; B, 0.98; 95% CI, 0.62-1.33), a larger gamma zone area at baseline (P < 0.001; beta, 0.14; B, 0.41; 95% CI, 0.17-0.64), and more time spent indoors studying (P = 0.015; beta, 0.10; B, 0.09; 95% CI, 0.02-0.17). Conclusions: The development and enlargement of the gamma zone in the temporal parapapillary region were associated with an optic disc rotation around the vertical disc axis as indicated by an increasing vertical-to-horizontal disc diameter ratio. These morphologic findings fit with the notion of a backward pull of the temporal peripapillary sclera through the optic nerve dura mater in axially elongated eyes.


Assuntos
Miopia/patologia , Atrofia Óptica/patologia , Disco Óptico/patologia , Adolescente , Grupo com Ancestrais do Continente Asiático , Comprimento Axial do Olho/patologia , Criança , China , Feminino , Humanos , Estudos Longitudinais , Masculino , Análise Multivariada , Estudos Prospectivos , Esclera/patologia
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