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1.
Oral Dis ; 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31793142

RESUMO

OBJECTIVE: To study the role of LncRNA CASC15 in oral squamous cell carcinoma. RESULTS: In the present study we showed that plasma CASC15 was up-regulated in stage I and II oral squamous cell carcinoma patients than in oral ulcer patients and healthy controls, while no significant correlation was found between oral ulcer patients and healthy controls. Up-regulation of plasma CASC15 distinguished oral squamous cell carcinoma patients from oral ulcer patients and healthy controls. LncRNA MEG3 was inversely correlated with CASC15 in oral squamous cell carcinoma tissues. In oral squamous cell carcinoma cells, CASC15 over-expression led to the inhibited expression of MEG3, MEG3 over-expression did not alter CASC15 expression. MEG3 over-expression decreased, while CASC15 over-expression increased the proliferation rate of oral squamous cell carcinoma cells. In addition, MEG3 over-expression attenuated the effects of CASC15 over-expression. CONCLUSION: Therefore, CASC15 over-expression may serve as a potential diagnostic biomarker for oral squamous cell carcinoma and CASC15 may promote oral squamous cell carcinoma cell proliferation by down-regulating MEG3.

2.
Sci Transl Med ; 11(516)2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31666403

RESUMO

Nonsteroidal anti-inflammatory drugs (NSAIDs) are among the most important causes of peptic ulcer disease in high-income countries. Proton pump inhibitors are the current standard treatment; however, safety and long-term adverse effects of using these drugs are attracting more and more concerns in recent years. Using a porcine model of NSAID-related gastric ulcer, we herein show that adipose-derived mesenchymal stem cells (ADMSCs) delivered by endoscopic submucosal injection promoted ulcer healing with less inflammatory infiltration and enhanced reepithelization and neovascularization at day 7 and day 21 when compared with the controls (saline injection). However, only few engrafted ADMSCs showed myofibroblast and epithelial cell phenotype in vivo, suggesting the ulcer healing process might be much less dependent on the stem cell transdifferentiation. Further experiment with submucosal injection of MSC-derived secretome revealed a therapeutic efficacy comparable to that of stem cell transplantation. Profiling analysis showed up-regulation of genes associated with inflammation, granulation formation, and extracellular matrix remodeling at day 7 after injection of MSC-derived secretome. In addition, the extracellular signal-regulated kinase/mitogen-activated protein kinase and the phosphoinositide-3-kinase/protein kinase B pathways were activated after injection of ADMSCs or MSC-derived secretome. Both signaling pathways were involved in mediating the major events critical to gastric ulcer healing, including cell survival, migration, and angiogenesis. Our data suggest that endoscopic submucosal injection of ADMSCs serves as a promising approach to promote healing of NSAID-related peptic ulcer, and the paracrine effectors released from stem cells play a crucial role in this process.

3.
Sci Rep ; 9(1): 17830, 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31780710

RESUMO

Programmed cell death 1 (PD-1) monoclonal antibodies have been approved by regulatory agencies for the treatment of various types of cancer, and the mechanism involves the restoration of T cell functions. We report herein the X-ray crystal structure of a fully human monoclonal antibody mAb059c fragment antigen-binding (Fab) in complex with the PD-1 extracellular domain (ECD) at a resolution of 1.70 Å. Structural analysis indicates 1) an epitope, comprising fragments from the C'D, BC and FG loops of PD-1, contributes to mAb059c interaction, 2) an unique conformation of the C'D loop and a different orientation of R86 enabling the capture of PD-1 by the antibody complementarity determining region (CDR) and the formation of one salt-bridge contact - ASP101(HCDR3):ARG86(PD-1), and 3) the contact of FG with light chain (LC) CDR3 is maintained by a second salt-bridge and two backbone hydrogen bonds. Interface analysis reveals that N-glycosylation sites 49, 74 and 116 on PD-1 do not contact mAb059c; while N58 in the BC loop is recognized by mAb059c heavy chain CDR1 and CDR2. Mutation of N58 attenuated mAb059c binding to PD-1. These findings and the novel anti-PD-1 antibody will facilitate better understanding of the mechanisms of the molecular recognition of PD-1 receptor by anti-PD-1 mAb and, thereby, enable the development of new therapeutics with an expanded spectrum of efficacy for unmet medical needs.

4.
Sci Rep ; 9(1): 17472, 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31767888

RESUMO

Control chart methods have been received much attentions in biosurvillance studies. The correlation between charting statistics or regions could be considerably important in monitoring the states of multiple outcomes or regions. In addition, the process variable distribution is unknown in most situations. In this paper, we propose a new nonparametric strategy for multivariate process monitoring when the distribution of a process variable is unknown. We discuss the EWMA control chart based on rank methods for a multivariate process, and the approach is completely nonparametric. A simulation study demonstrates that the proposed method is efficient in detecting shifts for multivariate processes. A real Japanese influenza data example is given to illustrate the performance of the proposed method.

5.
Cancer Cell ; 36(5): 498-511.e17, 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31715132

RESUMO

Signal transducer and activator of transcription 3 (STAT3) is an attractive cancer therapeutic target. Here we report the discovery of SD-36, a small-molecule degrader of STAT3. SD-36 potently induces the degradation of STAT3 protein in vitro and in vivo and demonstrates high selectivity over other STAT members. Induced degradation of STAT3 results in a strong suppression of its transcription network in leukemia and lymphoma cells. SD-36 inhibits the growth of a subset of acute myeloid leukemia and anaplastic large-cell lymphoma cell lines by inducing cell-cycle arrest and/or apoptosis. SD-36 achieves complete and long-lasting tumor regression in multiple xenograft mouse models at well-tolerated dose schedules. Degradation of STAT3 protein, therefore, is a promising cancer therapeutic strategy.

6.
Drug Dev Ind Pharm ; 45(12): 1879-1888, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31672067

RESUMO

The goal of this paper was to develop and evaluate dual component-loaded with the hydrophilic sinomenine hydrochloride (SH) and lipophilic cinnamaldehyde (CA) cubic liquid crystal gels for transdermal delivery. The gels was prepared with a vortex method using phytantriol/water (70:30, w/w) and characterized by polarized light microscopy, small-angle X-ray scattering and rheology. The inner structure of the gels were Pn3m cubic phase and exhibited a pseudoplastic fluid behavior. Furthermore, the in vitro release profile showed that the release behavior of the two drugs from cubic liquid crystal gels conformed to Higuchi equation and were dominated by Fick's diffusion (n < 0.45). The ex vivo penetration experiment indicated that dual components-loaded liquid crystal gels can enhance and extend the skin permeation of these two drugs, especially the ratio of SH to CA is 1: 0.5. Finally, transdermal mechanisms were evaluated using laser scanning confocal microscopy and attenuated total reflectance-fourier transform infrared, hinting that hydrophilic and lipophilic drugs weaken each other's transdermal velocity at the initial stage of penetration. In short, the dual drug-loaded liquid crystal gels was a promising strategy for transdermal applications in treatment of chronic disease.

7.
J Med Chem ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31747516

RESUMO

Signal transducer and activator of transcription 3 (STAT3) is a transcription factor and an attractive therapeutic target for cancer and other human diseases. Despite twenty-years of persistent research efforts, targeting STAT3 has been very challenging. We report herein the structure-based discovery of potent small-molecule STAT3 degraders based upon the proteolysis targeting chimera (PROTAC) concept. We first designed SI-109 as a potent, small-molecule inhibitor of the STAT3 SH2 domain. Employing ligands for cereblon/Cullin 4A E3 ligase and SI-109, we obtained a series of potent PROTAC STAT3 degraders, exemplified by SD-36. SD-36 induces rapid STAT3 degradation at low nanomolar concentrations in cells and fails to degrader other STAT proteins. SD-36 achieves nanomolar cell growth inhibitory activity in leukemia and lymphoma cell lines with high levels of phosphorylated STAT3. A single dose of SD-36 results in complete STAT3 protein degradation in xenograft tumor tissue and normal mouse tissues. SD-36 achieves complete and long-lasting tumor regression in the Molm-16 xenograft tumor model at well-tolerated dose-schedules. SD-36 is a potent, selective and efficacious STAT3 degrader.

8.
Sci Total Environ ; : 134621, 2019 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-31759711

RESUMO

Climate change and associated elevated atmospheric CO2 concentration and rising temperature have become a great challenge to agricultural production especially in arid and semiarid regions, and a great concern to scientists worldwide. Thus, it is very important to assess the response of crop growth and water productivity to climate change projections, which in turn can help devise adaptive strategies to mitigate their impact. An agro-hydrological model with well consideration of CO2 effects on both the stomatal conductance and leaf area was established. The model was well calibrated and validated using the data collected from the middle oasis of Heihe River basin, northwest China, which was selected as a typical arid region. Simulations of soil water contents and crop growth matched well with observations. Then various scenarios were designed with considering three climate change alternatives (RCP 2.6, RCP 4.5 and RCP 8.5) and three agricultural water-saving alternatives in the context of irrigation water availability being constant. Responses of crop growth and water productivity were predicted for thirty years from 2018 to 2047. As compared to current situation, there would be a reduction of 3.4-8.6% in crop yield during the period of 2018-2027 and an increase of 1.5-18.7% in crop yield during the period of 2028-2047 for seed corn, and an increase of 7.4-26.7% in crop yield during the period of 2018-2047 for spring wheat, respectively. Moreover, results showed an increase in water productivity ranged from 14.3% to 44.5% for seed corn and from 34.7% to 52.0% for spring wheat, respectively. Furthermore, adaptive strategies to climate change were recommended for the seed corn and spring wheat, respectively. Our results are expected to provide implications for devising adaptive strategies to changing environments in other arid and irrigation-fed areas.

9.
Angew Chem Int Ed Engl ; 58(50): 18276-18280, 2019 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-31609496

RESUMO

The salts [(BAC)2 PO][BF4 ] (5) and [(BAC)2 PO2 ][BF4 ] (4) (BAC=bis(diisopropylamino) cyclopropenylidene), consisting of the PO+ and PO2 + cations, respectively, coordinated to the singlet carbenes, have been prepared. Computational investigations reveal that the electronic structure of the PO+ cation is a hybrid between the charge-localized and charge-delocalized resonance forms, resulting in ambiphilic reactivity. Compound 5 reacts as a donor with the transition-metal complex K2 PtCl4 to furnish [[(BAC)2 PO]2 PtCl2 ][BF4 ]2 (6) and KCl. Remarkably, both 5 and 4 have shown to act as electrophiles undergoing reactions with fluoride anion, leading to [OPF2 ]- and (BAC)PO2 F, respectively.

10.
Int J Mol Sci ; 20(19)2019 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-31569691

RESUMO

Xanthones are important chemical constituents of Garcinia xanthochymus and varied bioactivities including cytotoxicity. However, their anti-tumor mechanism has remained unknown. Here, we isolated and identified a new xanthone named garciniaxanthone I (1) and five known compounds from the bark of G. xanthochymus. Their structures were elucidated by NMR analysis and HRESIMS. The anti-proliferation activities of all isolated compounds were evaluated on four human tumor cell lines (HepG2, A549, SGC7901, MCF-7). The results demonstrated that the anti-proliferation activity of xanthone was related to the number and location of prenyl groups. We further found that garciniaxanthone I (GXI) could induce HepG2 apoptosis and enhance the expression of cleaved caspase-8, caspase-9, and caspase-3. GXI could also increase Bax level and concurrently reduce the overexpression of Bcl-2, Bcl-XL, Mcl-1, and surviving in HepG2 cells. Moreover, GXI could inhibit cell migration of HepG2 cells by inhibiting the expressions of MMP-7 and MMP-9. In summary, our study suggests that GXI could induce HepG2 apoptosis via the mitochondrial pathway and might become a lead compound for liver cancer treatment.

11.
J Community Psychol ; 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31638727

RESUMO

This study aims to explore potential correlations between gender, loneliness, resilience, and depression simultaneously among Chinese drug users; and especially to investigate the mediating effect of resilience and the moderating effect of gender on the correlation between loneliness and depression. Participants of this study included 513 drug users (399 men and 114 women) who were under treatment in two compulsory drug treatment institutions in one Eastern Chinese city. All participants completed the UCLA (University of California, Los Angeles) loneliness scale, the Zung self-rating depression scale, and the Connor-Davidson resilience scale. A correlation matrix was constructed to analyze correlations between these three characteristics. Path analyses were performed in the PROCESS for SPSS in order to test the mediating effect of resilience on the association between loneliness and depression; while a series of hierarchical multiple regressions were conducted in SPSS 22.0 to test the moderating effect of gender on the association between loneliness and depression. Compared to male drug users, female ones reported higher average scores on loneliness and depression, which indicated that female drug users suffered severer loneliness and depression. Resilience partially mediates the link between loneliness and depression and gender acts as a moderator for the relationship between loneliness and depression.

12.
J Am Chem Soc ; 141(42): 16971-16982, 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31557435

RESUMO

The phosphaaluminirenes HC[(CMe)(NDipp)]2Al[C(R)═P] (Dipp = 2,6-i-Pr2C6H3, R = tBu or adamantyl) 2 and 3, featuring an unsaturated three-membered AlCP ring, have been synthesized as crystalline solids via a [1 + 2] cycloaddition reaction of the aluminum(I) complex HC[(CMe)(NDipp)]2Al (1) with phosphaalkynes. Computational investigations infer three-centered 2π-electron aromaticity of the AlCP rings. Compound 3 is readily protonated by tBuOH to induce a ring-opening σ-bond metathesis, giving an alumina-substituted P-hydrogeno phosphaalkene 4. Remarkably, the high strain of the AlCP ring of 3 allows for facile ring enlargement in reactions with CyNC, bis(diisopropylamino) cyclopropenylidene (BAC), elemental Se, Ph2CO, PhCH═CHCOPh, and PhCN at room temperature. These furnish a series of unprecedented main group heterocycles 5-10 with the C═P unsaturated bonds remaining intact. The mechanisms are considered in light of thorough density functional theory (DFT) calculations.

13.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 33(10): 1310-1319, 2019 Oct 15.
Artigo em Chinês | MEDLINE | ID: mdl-31544445

RESUMO

Objective: To investigate the heterotopic osteogenesis of tissue engineered bone using the co-culture system of vascular endothelial cells (VECs) and adipose-derived stem cells (ADSCs) as seed cells. Methods: The partially deproteinized biological bone (PDPBB) was prepared by fibronectin combined with partially deproteinized bone (PDPB). The ADSCs of 18-week-old Sprague Dawley (SD) rats and VECs of cord blood of full-term pregnant SD rats were isolated and cultured. Three kinds of tissue engineered bone were constructed in vitro: PDPBB+VECs (group A), PDPBB+ADSCs (group B), PDPBB+co-cultured cells (VECs∶ADSCs was 1∶1, group C), and PDPBB was used as control group (group D). Scanning electron microscopy was performed at 10 days after cell transplantation to observe cell adhesion on scaffolds. Forty-eight 18-week-old SD rats were randomly divided into groups A, B, C, and D, with 12 rats in each group. Four kinds of scaffolds, A, B, C, and D, were implanted into the femoral muscle bags of rats in corresponding groups. The animals were killed at 2, 4, 8, and 12 weeks after operation for gross observation, HE staining and Masson staining histological observation, and the amount of bone collagen was measured quantitatively by Masson staining section. Results: Scanning electron microscopy showed that the pores were interconnected in PDPB materials, and a large number of lamellar protein crystals on the surface of PDPBB modified by fibronection were loosely attached to the surface of the scaffold. After 10 days of co-culture PDPBB and cells, a large number of cells attached to PDPBB and piled up with each other to form cell clusters in group C. Polygonal cells and spindle cells were mixed and distributed, and some cells grew along bone trabeculae to form cell layers. Gross observation showed that the granulation tissue began to grow into the material pore at 2 weeks after operation. In group C, a large number of white cartilage-like substances were gradually produced on the surface of the material after 4 weeks, and the surface of the material was uneven. At 12 weeks, the amount of blood vessels on the surface of group A increased, and the material showed consolidation; there was a little white cartilage-like material on the surface of group B, but the pore size of the material did not decrease significantly; in group D, the pore size of the material did not decrease significantly. Histological observation showed that there was no significant difference in the amount of bone collagen between groups at 2 weeks after operation ( F=2.551, P=0.088); at 4, 8, and 12 weeks after operation, the amount of bone collagen in group C was significantly higher than that in other 3 groups, and that in group B was higher than that in group D ( P<0.05); there was no significant difference between group A and groups B, D ( P>0.05). Conclusion: The ability of heterotopic osteogenesis of tissue engineered bone constructed by co-culture VECs and ADSCs was the strongest.


Assuntos
Células Endoteliais , Osteogênese , Animais , Células Cultivadas , Técnicas de Cocultura , Células-Tronco Mesenquimais , Ratos , Ratos Sprague-Dawley , Células-Tronco , Engenharia Tecidual , Tecidos Suporte
14.
Lab Chip ; 19(20): 3438-3447, 2019 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-31556903

RESUMO

Paper-based microfluidics (lab on paper) emerges as an innovative platform for building small-scale devices for sensing, diagnosis, and energy storage/conversions due to the power-free fluidic transport capability of paper via capillary action. Herein, we report for the first time that paper-based microfluidic concept can be employed to fabricate high-performing aluminum-air batteries, which entails the use of a thin sheet of fibrous capillary paper sandwiched between an aluminum foil anode and a catalyst coated graphite foil cathode without using any costly air electrode or external pump device for fluid transport. The unique microfluidic configuration can help overcome the major drawbacks of conventional aluminum-air batteries including battery self-discharge, product-induced electrode passivation, and expensive and complex air electrodes which have long been considered as grand obstacles to aluminum-air batteries penetrating the market. The paper-based microfluidic aluminum-air batteries are not only miniaturized in size, easy to fabricate and cost-effective, but they are also capable of high electrochemical performance. With a specific capacity of 2750 A h kg-1 (@20 mA cm-2) and an energy density of 2900 W h kg-1, they are 8.3 and 12.6 times higher than those of the non-fluidic counterpart and significantly outperform many other miniaturized energy sources, respectively. The superior performance of microfluidic aluminum-air batteries originates from the remarkable efficiency of paper capillarity in transporting electrolyte along with O2 to electrodes.

15.
Int J Biol Macromol ; 141: 876-884, 2019 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-31476399

RESUMO

The physical mechanics and structural properties of composite films based on chitosan and syringic acid (SA) were studied in this study. The results indicated that the addition of SA made the chitosan-SA films exhibited higher density, water solubility and opacity, but the water vapor permeability and water content were decreased. In addition, Chitosan-SA films had a significant antibacterial effect on test bacteria. The surface and cross-section of chitosan-SA films were more uniform and smoother when combined with 0.25% and 0.5% 0f SA. The FT-IR and XRD spectra of the chitosan-SA film indicated that the interaction between chitosan and SA may be non-covalent, and DSC indicated that the thermal stability of the composite film was reduced. In summary, the modified color, increased bacteriostatic and water-blocking properties, as well as the slight changes in mechanical properties indicated that the addition of SA may contribute to extend the shelf life of the food. Thus, chitosan-SA films incorporating 0.25% and 0.5% of SA can be further explored as active packing materials for food preservation. The composite film was used for the preservation of quail egg coatings, and it was found that 0.25% and 0.5% of the coating film had a good preservation effect.

16.
J Exp Clin Cancer Res ; 38(1): 401, 2019 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-31511060

RESUMO

BACKGROUND: Colon cancer is the second leading cancer worldwide. Recurrent disease and chemotherapeutic drug resistance are very common in the advanced stage of colon cancer. ATP-citrate lyase (ACLY), the first-step rate-controlling enzyme in lipid synthesis, is elevated in colon cancer. However, it remains unclear about the exact role of ACLY in the development of colon cancer metastasis. METHODS: To evaluate the role of ACLY in colon cancer metastasis, we performed cell migration and invasion assays in two ACLY-deficient colon cancer cell lines. Colon cancer mouse model is used to examine ACLY's effects on colon metastasis potentials in vivo. We analyzed the correlation between ACLY and CTNNB1 protein in 78 colon cancer patients by Pearson correlation. To finally explore the relationship of ACLY and CTNNB1, we used western blots, migration and invasion assays to confirm that ACLY may regulate metastasis by CTNNB1. RESULTS: Our data showed that the abilities of cell migration and invasion were attenuated in ACLY-deficient HCT116 and RKO cell lines. Furthermore, we describe the mechanism of ACLY in promoting colon cancer metastasis in vitro and in vivo. ACLY could stabilize CTNNB1 (beta-catenin 1) protein by interacting, and the complex might promote CTNNB1 translocation through cytoplasm to nucleus, subsequently promote the CTNNB1 transcriptional activity and migration and invasion abilities of colon cancer cells. Immunohistochemical analysis of 78 colon cancer patients showed that the high expression levels of ACLY and CTNNB1 protein was positively correlated with metastasis of colon cancer. CONCLUSIONS: These results shed new light on the molecular mechanism underlying colon cancer metastasis, which might help in improving therapeutic efficacy.

17.
Pathol Oncol Res ; 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31482399

RESUMO

Clear cell odontogenic carcinoma (CCOC) is a rare odontogenic tumor associated with aggressive clinical behavior, metastasis and low survival. To date, only 67 cases have been described in the English language literature, and an understanding of the behavior of CCOC has been based on limited case reports. The aim of the research was to further reveal the features of CCOC. We report 5 new cases of CCOC, with a mean age of 52.4 years. The clinical and histopathologic data of the disease obtained from earlier literature (95 cases) and the 5 new cases were analyzed. Data were extracted, including demographics, histopathologic findings, clinical presentation, primary treatment and outcomes. Immunohistochemical results revealed that the cancer is positive for AE1/AE3, EMA and CK19, negative for smooth muscle actin SMA, Vim and S-100. EWSR1 translocation was also observed in the new cases, which may help in the diagnosis of CCOC. Metastases of CCOC were rare, but the local recurrence rate of CCOC rose to 42%. The best treatment for patients with CCOC is wide local excision combined with regional lymph node dissection.

18.
Water Res ; 167: 115091, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31561089

RESUMO

Cyanobacterial surface scum (here defined as visible Cyanobacteria colonies accumulating at the lake surface) is a harmful phenomenon that negatively affects water quality, human and animal health. Colony-forming Microcystis is one of the most important and ubiquitous genera that can suddenly accumulate at water surfaces. Turbulent water motion, e.g., generated by wind, can vertically disperse this scum layer, which later can re-establish by upward migration of Microcystis colonies. However, the role of wind-generated turbulence in scum formation and development is still poorly understood. Here we present results from a laboratory mesocosm study where we analysed the processes of scum formation and its response to wind-generated turbulence at low wind speed (≤3.6 m s-1). Microcystis colony size and flow velocity at the water surface and in the bulk water were measured using a microscope camera and particle tracking velocimetry. The surface scum formed by aggregation of colonies at the water surface, where they formed loose clusters of increasing size. The presence of large colony aggregations or of a surface film determined the stability of the scum layer. For the largest applied wind speed, most of the aggregations were broken down to sizes <2 mm, which were dispersed to the bulk water. The surface scum recovered quickly from such disturbances after the wind speed decreased. We further observed reduced momentum transfer from wind to water with the growing scum layer. The presence of the scum increased the threshold wind speed for the onset of flow and reduced the flow velocities that were generated above that threshold. This effect was likely caused by the presence of a film of surface-active material at the water surface (surface microlayer), which is related to the presence of Microcystis. Both the small-scale turbulence and surface microlayer might play an important, yet largely unexplored role in Microcystis surface scum development in aquatic ecosystems. Improved understanding of the interplay of both processes will be instrumental for improving current mechanistic models for predicting surface bloom dynamics.


Assuntos
Cianobactérias , Microcystis , Animais , Ecossistema , Lagos , Água
19.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 31(7): 890-895, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31441416

RESUMO

OBJECTIVE: To explore the relationship between training burnout, sleep quality and heat regulation response, severe heatstroke in people performed 5-km armed cross-country training. METHODS: 600 male officers and soldiers who participated in 5-km armed cross-country training in summer from 2017 to 2018 were enrolled. All trainees participated in 5-km armed cross-country training in environment with ambient temperature > 32 centigrade and (or) humidity > 65%. They were divided into two groups according to whether severe heatstroke occurred during 5-km armed cross-country training. The age, military age, body mass index (BMI), physical fitness score, external environment (such as ambient temperature, relative humidity, wind speed, heat index), training burnout score and Pittsburgh sleep quality index scale (PSQI) score, heart rate (HR), core temperature (Tc), sweating volume and serum Na+, K+, Cl- levels were compared between the groups. The risk factors of severe heatstroke during 5-km armed cross-country training were screened by binary multivariate Logistic regression analysis. RESULTS: There were 26 cases of severe heatstroke in 600 trainees who participated in 5-km armed cross-country training, with an incidence of 4.33%. There was no significant difference in age, military age, BMI, physical fitness score and external environment of 5-km armed cross-country training between people with or without severe heatstroke. Compared with those without severe heatstroke, the dimensions of training burnout and the total average scores of training burnout of severe heatstroke personnel before 5-km armed cross-country training were increased significantly (physical and mental exhaustion score: 12.4±2.5 vs. 9.4±3.5, training alienation score: 8.8±2.8 vs. 5.8±2.3, low sense of achievement score: 8.2±2.7 vs. 5.6±2.3, total score of training burnout: 9.8±3.2 vs. 6.9±3.2, all P < 0.01), all factors except daytime dysfunction (DD) of PSQI and total PSQI score were also increased significantly [sleep quality (SQ) score: 1.0 (1.0, 2.0) vs. 1.0 (1.0, 1.0), fall asleep time (SL) score: 2.0 (1.0, 3.0) vs. 1.0 (1.0, 1.0), sleep time (SH) score: 1.0 (0.8, 2.0) vs. 1.0 (0, 1.0), sleep efficiency (SE) score: 1.0 (0, 1.0) vs. 0 (0, 0.8), sleep disorder (SD) score: 2.0 (1.0, 3.0) vs. 1.0 (0, 2.0), total PSQI score: 1.0 (1.0, 2.0) vs. 1.0 (0, 1.0), all P < 0.01], HR was increased significantly at onset (bpm: 120.00±10.57 vs. 86.49±14.91, P < 0.01), Tc was increased significantly (centigrade: 41.46±0.57 vs. 37.97±0.83, P < 0.01), serum electrolyte contents were decreased significantly [Na+ (mmol/L): 130.54±5.97 vs. 143.15±10.56, K+ (mmol/L): 3.72±0.44 vs. 4.37±0.50, Cl- (mmol/L): 97.58±4.80 vs. 102.10±2.39, all P < 0.01], and the amount of sweat during training was increased significantly (g: 395.81±16.16 vs. 371.88±40.76, P < 0.01). Binary multivariate Logistic regression analysis showed that total score of training burnout [odd ratio (OR) = 0.653, 95% confidence interval (95%CI) = 0.563-0.757], total PSQI score (OR = 0.693, 95%CI = 0.525-0.916), HR (OR = 0.871, 95%CI = 0.838-0.908), Tc (OR = 0.088, 95%CI = 0.043-0.179), sweating volume (OR = 0.988, 95%CI = 0.979-0.997), Na+ (OR = 1.112, 95%CI = 1.069-1.158), K+ (OR = 13.900, 95%CI = 5.343-36.166), Cl- (OR = 1.393, 95%CI = 1.252-1.550) were independent risk factors for severe heatstroke during 5-km armed cross-country training (all P < 0.01). CONCLUSIONS: Increase in training burnout, total PSQI score, excessive changes of body heat regulation response and excessive loss of Na+, K+, Cl- in serum are independent risk factors for severe heatstroke during 5-km armed cross-country training under the same conditions with high temperature and humidity environment.


Assuntos
Esgotamento Psicológico , Golpe de Calor , Temperatura Alta , Militares , Frequência Cardíaca , Humanos , Masculino , Sono , Estresse Fisiológico
20.
Genomics ; 2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31376529

RESUMO

Deltamethrin (DM) is widely used in a variety of pest control, resulting in serious drug resistance. Keap1-Nrf2-ARE is the antioxidant stress pathway. We identified 268 genes differentially expressed (DEGs) in Drosophila Kc cells treated with DM, including up-regulated 180 genes and down-regulated 88 genes compared with the control group (fold-change≥2, qValue≤0.001) by RNA-seq, they are mainly linked to metabolic process, stimulation response, immune system process. When the cells are treated with DM in the case of overexpression of the Keap1 gene, the cytochrome P450 family genes were significantly down-regulated, and some diseases-related genes and non-coding genes also changed. Our data shown that Keap1-Nrf2-ARE pathway may play an important role in DM stress, which will provide a new direction for studying the mechanism of insect resistance.

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