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1.
Chemosphere ; 240: 124941, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31726615

RESUMO

Ametryn (AMT), one of the most widely used herbicides in agriculture, has been frequently detected as a micropollutant in many aquatic environments. AMT residue not only pollutes water but also acts as a precursor for the production of disinfection by-products (DBPs). This study systematically investigated the fate of AMT during the UV/chlorine process. It was observed that the combination of UV irradiation and chlorination degraded AMT synergistically. The results of the radical quenching experiments suggested that AMT degradation by the UV/chlorine process involved the participation of UV photolysis, hydroxyl radical (OH) reactions, and reactive chlorine species (RCS) reactions, which accounted for 45.4%, 36.4%, and 14.5% of the degradation, respectively. Moreover, we found that Cl- 2 was an important reactive radical for AMT degradation. The chlorine dose, pH, coexisting anions (Cl- and HCO3-), and natural organic matter (NOM) were found to affect AMT degradation during the UV/chlorine process. Nineteen predominant intermediates/products of AMT degradation during UV/chlorine process were identified, including atrazine. Moreover, the corresponding transformation pathways were proposed, including electron transfer, bond cleavage (C-S, C-N), radical (OH, Cl and Cl- 2) reactions, and subsequent hydroxylation. The toxicity tests with Vibrio fischeri on AMT degradation suggested that more DBPs were generated by UV/chlorine-treated AMT, which possessed higher acute toxicity than AMT did. Although the UV/chlorine process evidently promoted the AMT degradation, optimization of process parameters may reduce the DBP production and merits further investigation.

2.
Molecules ; 24(22)2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31718071

RESUMO

Psoralen (P) and isopsoralen (IP) are the main active ingredients in the dried fruit of Psoralen corylifolia L. (PC), with a wide range of pharmacology activities. The intestinal bacteria biotransformation plays a central role in the metabolism of the complex ingredients in traditional Chinese medicine (TCM). Our study aimed to investigated the metabolic profile of P and IP in the intestinal condition, co-cultured with human fecal bacteria anaerobically. Four bio-transforming products were obtained, including 6,7-furano-hydrocoumaric acid (P-1) and 6,7-furano-hydro- coumaric acid methyl ester (P-2), which transformed from P, and 5,6-furano-hydrocoumaric acid (IP-1) and 5,6-furano-hydrocoumaric acid methyl ester (IP-2), which were transformed from IP. It is worth mentioning that IP-2 is a new compound that has not been published. Their structures were analyzed based on their spectroscopic data. Moreover, a highly sensitive ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was used to characterize the metabolic pathways of P, IP, and their bio-transforming products in the reaction samples. In addition, the dampening effects against the oxidative stress of P, IP, and their bio-transforming products by human intestinal flora were estimated in vitro via the human colorectal cells (HCT116) and heterogeneous human epithelial colorectal adenocarcinoma cells (Caco-2) cell lines. The results showed that the metabolites have stronger activity than P and IP, which possibly provides a basis for elucidating the treating mechanisms of PC extract against inflammatory bowel disease.

3.
Nat Plants ; 5(11): 1184-1193, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31712753

RESUMO

Bicarbonate transporters play essential roles in pH homeostasis in mammals and photosynthesis in aquatic photoautotrophs. A number of bicarbonate transporters have been characterized, among which is BicA-a low-affinity, high-flux SLC26-family bicarbonate transporter involved in cyanobacterial CO2-concentrating mechanisms (CCMs) that accumulate CO2 and improve photosynthetic carbon fixation. Here, we report the three-dimensional structure of BicA from Synechocystis sp. PCC6803. Crystal structures of the transmembrane domain (BicATM) and the cytoplasmic STAS domain (BicASTAS) of BicA were solved. BicATM was captured in an inward-facing HCO3--bound conformation and adopts a '7+7' fold monomer. HCO3- binds to a cytoplasm-facing hydrophilic pocket within the membrane. BicASTAS is assembled as a compact homodimer structure and is required for the dimerization of BicA. The dimeric structure of BicA was further analysed using cryo-electron microscopy and physiological analysis of the full-length BicA, and may represent the physiological unit of SLC26-family transporters. Comparing the BicATM structure with the outward-facing transmembrane domain structures of other bicarbonate transporters suggests an elevator transport mechanism that is applicable to the SLC26/4 family of sodium-dependent bicarbonate transporters. This study advances our knowledge of the structures and functions of cyanobacterial bicarbonate transporters, and will inform strategies for bioengineering functional BicA in heterologous organisms to increase assimilation of CO2.

4.
Psych J ; 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31722451

RESUMO

Involuntary mental time travel (MTT) refers to the phenomenon of mentally reliving past experiences or pre-living possible future events in an involuntary form. Few studies have explored involuntary MTT in individuals with schizotypal personality features. The present study aimed to first explore the psychometric properties of the Involuntary Autobiographic Memory Inventory (IAMI) in a Chinese sample (Study 1), and then to explore whether individuals with schizotypal personality features experience involuntary MTT more frequently than individuals without schizotypal features. Moreover, the study explored whether the aberrant frequency of involuntary MTT is correlated with positive schizotypal features (Study 2). The results showed that the IAMI had good structural validity and reliability in a Chinese sample. Individuals with schizotypal traits reported a significantly higher frequency, less positive emotion, and stronger emotional intensity for both involuntary memories and future thoughts compared with individuals without schizotypal features. Further analyses in individuals with schizotypal personality features showed that the frequencies of both involuntary memories and future thoughts were significantly correlated with positive schizotypal traits. These results have potential theoretical and clinical implications for a comprehensive understanding of involuntary MTT among individuals with schizotypal personality features.

5.
Sci Total Environ ; 699: 134395, 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31678886

RESUMO

Catalytic ozonation is an efficient process that can be utilized to degrade recalcitrant organics. Char-clay composite derived from refinery spent bleaching earth (SBE) is an economical and readily available catalyst that can be used during the ozonation treatment of recalcitrants in wastewater. Four catalysts of SBE-N2-650, SBE-N2-850, SBE-O2-650, and SBE-O2-850 were prepared by heating the SBE at 650 and 850 °C under N2 or O2 conditions. High surface OH sites in the SBE-N2-650 and SBE-O2-650 relative to the SBE-N2-850 and SBE-O2-850 resulted in an increase in catalytic activity. Additional carbon (C), that existed in the SBE-N2-650 and SBE-N2-850, had a positive effect on catalytic activity. The SBE-N2-650 exhibited the highest activity among those prepared catalysts. During catalytic ozonation, the SBE-N2-650 increased the mineralization rate of benzoic acid by 36% when compared with single ozonation. Molecular ozone was decomposed at the surface active sites on SBE-N2-650, generating active •OH, •O2-, or 1O2 species. Gas and liquid products having calorific values that are generated during SBE-N2-650 preparation can be further utilized. This study introduces a potential use of SBE for the ozonation treatment of recalcitrant wastewaters.

6.
Clin Nutr ; 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31694759

RESUMO

BACKGROUND: Previous clinical and animal studies suggested that medium-chain triglycerides (MCT) might be an alternative energy substrate for the brain and might benefit patients with Alzheimer's disease (AD), but the clinical evidence is not substantial or totally convincing. OBJECTIVE: To investigate the effects of MCT on cognitive ability in patients with mild to moderate AD and explore the changes in peripheral blood metabolomics. METHODS: A double-blind, randomized, placebo-controlled crossover study was undertaken in 53 mild to moderate AD patients. Participants were randomized between two sequences (placebo followed by MCT or MCT followed by placebo) and took MCT jelly or placebo jelly (canola oil) by mouth three times daily (total daily fat dose: 17.3 g MCT, or 19.7 g canola oil) for 30 days per phase. The primary outcome was cognition as measured by the Alzheimer's Disease Assessment Scale-Cognitive Subscale, Chinese version (ADAS-Cog-C). The secondary outcome was self-care as measured by the activities of daily living scale (ADL) and changes in plasma metabolites. RESULTS: This study showed a significant (p < 0.01) reduction in ADAS-Cog-C scores between the MCT (2.62 points below baseline) and placebo interventions (2.57 points above baseline). Data from 46 (86.8%) APOE4-/- subjects who completed the entire study were analyzed. Changes in ADL scores were not significantly different between the MCT and placebo interventions (p > 0.05). The concentrations of TC, HDL-C, ß-hydroxybutyrate and acetoacetate were significantly higher in the MCT group than in the placebo group (p < 0.05). Lysophosphatidylcholine 16:0 (LysoPC (16:0)), LysoPC (P-18:0), LysoPC (P-18:1(9Z)), LysoPC (20:2(11Z,14Z)), and LysoPC (22:5(4Z,7Z,10Z,13Z,16Z)) were significantly increased after MCT intervention, and the concentrations of LysoPC (18:0), palmitic acid, linoleic acid, oleic acid, and 7,12-dimethylbenz[a]anthracene were significantly decreased (p < 0.05), whereas no significant changes appeared after the placebo intervention. Androstenedione concentration increased after placebo intervention. Furthermore, a significant negative correlation was observed between changes in LysoPC (P-18:1(9Z)) and ADAS-Cog-C scores after MCT intervention (r = -0.1472, p < 0.05). CONCLUSIONS: MCT had positive effects on cognitive ability in mild to moderate AD patients with APOE4-/-. These effects of MCT might be related to the metabolism of LysoPC, oleic acid, linoleic acid and palmitic acid, in addition to the ketogenic effect. STUDY ID NUMBER: ChiCTR-IOR-16009737. REGISTRY WEBSITE: WHO ICTRP Search Portal - http://apps.who.int/trialsearch/Default.aspx.

7.
Ann Hum Genet ; 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31691269

RESUMO

Single-nucleotide polymorphisms (SNPs) in the UHRF gene have been shown to be associated with systemic lupus erythematosus (SLE) in European and Hong Kong Chinese, but statistically significant evidence for association has not been found in a mainland Han Chinese population. Therefore, we selected SNP rs13205210 located in UHRF1BP1 as a candidate association from our previously published genome-wide association study (GWAS) data of SLE (1,047 cases and 1,205 controls from a mainland Han Chinese population) to explore the association between the UHRF1BP1 gene and SLE. We conducted a large-scale replication study in an additional independent sample of 3,509 cases and 8,246 controls from a mainland Han Chinese population. Real-time PCR was used to determine gene expression differences in peripheral blood mononuclear cells (PBMCs) from cases and controls. As a result, we replicated the association between the UHRF1BP1 gene and SLE (rs13205210, missense, Pmeta   = 2.26E-17, odds ratio = 1.41) by a meta-analysis of our previous GWAS and this replication study involving a total of 4,556 cases and 9,451 controls. The UHRF1BP1 mRNA expression level in PBMCs was significantly decreased in patients with SLE compared with that in healthy controls. SNP rs13205210 exhibited an expression quantitative trait loci effect on the UHRF1BP1 gene in PBMCs from patients. In conclusion, this study not only suggests that the UHRF1BP1 gene was associated with SLE in a mainland Han Chinese population, but also implied that it might be a common genetic factor contributing to SLE susceptibility in multiple populations.

8.
J Med Virol ; 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31674680

RESUMO

Poliovirus (PV) is a member of the species Enterovirus C (EV-C), which may cause irreversible paralysis and death. So, for the purpose of analyzing the evolution of PV2 to help in eradicating PVs globally, a recombination analysis was performed to verify all viral genomes of EV-C, and we found 13 putative recombination events that produced PV1, 14 recombination events that can give rise to PV2, and 9 events that can lead to PV3. By analyzing our findings, we found that PV2 was involved in 25 of 36 PV recombination events, whereas coxsackievirus A (CVA) strains were involved in 12 of 36 PV recombination events, indicating that PV2 and CVAs play major roles in the natural recombination of PV. In addition, we found 11 of 36 breakpoint positions located in 2A region, which is the most active region of the recombination events.

9.
Food Chem ; : 125865, 2019 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-31757488

RESUMO

Although infant formula represents an important human milk substitute, it has been shown to contain many different lipid structures compared to human milk. In this study, the lipidome and fatty acid composition of human, bovine and caprine milk were analyzed and compared. A total of 13 lipid classes (including TG, DG, SM, PC, Cer, HexCer, Hex2Cer, PE, PG, PS, PI, PA and CL) were analyzed. Human milk was richer in TG containing LA, SM containing ULCFA and PLs containing ARA, DHA and DGLA. Moreover, there were no differential DG and SM species between human and caprine milk. A total of 215 and 147 lipids were identified as potential biomarkers that could be used to further analyze differences in the biological properties of human, bovine and caprine milk. This comprehensive analysis will hopefully help in the design of infant formula more suitable for Chinese babies.

10.
Target Oncol ; 14(6): 719-728, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31691892

RESUMO

BACKGROUND: Droplet digital polymerase chain reaction (ddPCR) is an emerging technology for quantitative cell-free DNA oncology applications. However, a ddPCR assay for the epidermal growth factor receptor (EGFR) p.Thr790Met (T790M) mutation suitable for clinical use remains to be established with analytical and clinical validations. OBJECTIVE: We aimed to develop and validate a new ddPCR assay to quantify the T790M mutation in plasma for monitoring and predicting the progression of advanced non-small-cell lung cancer (NSCLC). METHODS: Specificity of the ddPCR assay was evaluated with genomic DNA samples from healthy individuals. The inter- and intraday variations of the assay were evaluated using mixtures of plasmid DNA containing wild-type EGFR and T790M mutation sequences. We assessed the clinical utility of the T790M assay in a multicenter prospective study in patients with advanced NSCLC receiving tyrosine kinase inhibitor (TKI) treatment by analyzing longitudinal plasma DNA samples. RESULTS: We set the criteria for a positive call when the following conditions were satisfied: (1) T790M mutation frequency > 0.098% (3 standard deviations above the background signal); (2) at least two positive droplets in duplicate ddPCR reactions. Among the 62 patients with advanced NSCLC exhibiting resistance to TKI treatment, 15 had one or more serial plasma samples that tested positive for T790M. T790M mutation was detected in the plasma as early as 205 days (median 95 days) before disease progression, determined by imaging analysis. Plasma T790M concentrations also correlated with intervention after disease progression. CONCLUSIONS: We developed a ddPCR assay to quantify the T790M mutation in plasma. Quantification of longitudinal plasma T790M mutation may allow noninvasive assessment of drug resistance and guide follow-up treatment in TKI-treated patients with NSCLC. TRIAL REGISTRATION: Clinical Trials.gov identifier: NCT02804100.

11.
Mol Med Rep ; 20(5): 4059-4066, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31702028

RESUMO

The present study aimed to explore the role of the PTEN/Akt/mTOR signaling pathway in the neurite outgrowth and apoptosis of cortical neurons. Cortical neurons were seeded on or adjacent to chondroitin sulfate proteoglycans. The length, number and crossing behavior of the neurites were calculated. Immunohistochemical staining and TUNEL data were analyzed. Neurites treated with PTEN inhibitor exhibited significant enhancements in elongation, initiation and crossing abilities when they encountered chondroitin sulfate proteoglycans in vitro. These effects disappeared when the PTEN/Akt/mTOR signaling pathway was blocked. Neurons exhibited significant enhancements in survival ability following PTEN inhibition. The present study demonstrated that PTEN inhibition can promote axonal elongation and initiation in cerebral cortical neurons, as well as the ability to cross the chondroitin sulfate proteoglycan border. In addition, PTEN inhibition is useful for protecting the neuron from apoptosis. The PTEN/Akt/mTOR signaling pathway is an important signaling pathway.

12.
BMC Genomics ; 20(1): 856, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31726968

RESUMO

BACKGROUND: Non-coding RNAs (ncRNAs), including microRNAs (miRNAs), long ncRNAs (lncRNAs) and circular RNAs (circRNAs), accomplish remarkable variety of biological functions. However, the composition of ncRNAs and their interactions with coding RNAs in modulating and controlling of cellular process in plants is largely unknown. Using a diverse group of high-throughput sequencing strategies, the mRNA, miRNA, lncRNA and circRNA compositions of tobacco (Nicotiana tabacum) roots determined and their alteration and potential biological functions in response to topping treatment analyzed. RESULTS: A total of 688 miRNAs, 7423 non-redundant lncRNAs and 12,414 circRNAs were identified, among which, some selected differentially expressed RNAs were verified by quantitative real-time PCR. Using the differentially expressed RNAs, a co-expression network was established that included all four types of RNAs. The number of circRNAs identified were higher than that of miRNAs and lncRNAs, but only two circRNAs were present in the co-expression network. LncRNAs appear to be the most active ncRNAs based on their numbers presented in the co-expression network, but none of them seems to be an eTM (endogenous Target Mimicry) of miRNAs. Integrated with analyses of sequence interaction, several mRNA-circRNA-miRNA interaction networks with a potential role in the regulation of nicotine biosynthesis were uncovered, including a QS-circQS-miR6024 interaction network. In this network miR6024 was significantly down-regulated, while the expression levels of its two targets, circQS and its host gene QS, were sharply increased following the topping treatment. CONCLUSIONS: These results illustrated the transcriptomic profiles of tobacco roots, the organ responsible for nicotine biosynthesis. mRNAs always play the most important roles, while ncRNAs are also expressed extensively for topping treatment response, especially circRNAs are the most activated in the ncRNA pool. These studies also provided insights on the coordinated regulation module of coding and non-coding RNAs in a single plant biological sample. The findings reported here indicate that ncRNAs appear to form interaction complex for the regulation of stress response forming regulation networks with transcripts involved in nicotine biosynthesis in tobacco.

13.
Nanotechnology ; 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31751976

RESUMO

Pathogenic bacterial infection, especially in the wound, may threaten human health. Developing new antibacterial materials for wound healing is still urgent. Metal nanoclusters have been explored as a novel antibacterial agent. Herein, biomolecule gelatin was chosen as a substrate and functionalized with gold/silver clusters for bacterial killing. Through a simple amidation reaction, gold/silver clusters were successfully conjugated in gelatin substrate to obtain Au/Ag@gelatin sponge. The presence of gold/silver clusters modified the porous structure of gelatin, and thus the water absorption and water retention of Au/Ag@gelatin sponge were enhanced. More importantly, the gold/silver clusters show aggregation-enhanced emission and strong reactive oxygen generation, that endow Au/Ag@gelatin sponge good antibacterial property. The good physical performance and favorable bactericidal activity of Au/Ag@gelatin sponge suggest their potential for applying as a wound dressing.

14.
Nanotechnology ; 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31766028

RESUMO

In this work, the effects of Al incorporation into ZrO2 as the gate dielectrics of few-layered MoS2 field-effect transistors (FETs) on their electrical properties are investigated, and a large improvement in device performance is achieved by the Al-doped ZrO2 gate dielectric with Zr:Al = 1:1. The relevant MoS2 transistor exhibits the best electrical characteristics: high carrier mobility of 40.6 cm2/Vs (41% higher than that of the control sample, and an intrinsic mobility of 68.0 cm2/Vs), small subthreshold swing of 143 mV/dec, high on/off current ratio of 6×106 and small threshold voltage of 0.71 V. These are attributed to the facts that (i) Al incorporation into ZrO2 can decrease its oxygen vacancies; densify the dielectric film; and smooth the gate dielectric surface, thus reducing the traps at/near the Zr0.5Al0.5Oy/MoS2 interface and the gate leakage current; (ii) adjusting the dielectric constant of the gate dielectric to an appropriate value, which could achieve a reasonable trade-off between the gate screening effect on the Coulomb-impurity scattering and the surface optical phonon scattering. These results demonstrate that the optimized Zr0.5Al0.5Oy is a potential gate dielectric material for MoS2 FET applications.

15.
Proc Natl Acad Sci U S A ; 116(47): 23850-23858, 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31685622

RESUMO

Increasing maize grain yield has been a major focus of both plant breeding and genetic engineering to meet the global demand for food, feed, and industrial uses. We report that increasing and extending expression of a maize MADS-box transcription factor gene, zmm28, under the control of a moderate-constitutive maize promoter, results in maize plants with increased plant growth, photosynthesis capacity, and nitrogen utilization. Molecular and biochemical characterization of zmm28 transgenic plants demonstrated that their enhanced agronomic traits are associated with elevated plant carbon assimilation, nitrogen utilization, and plant growth. Overall, these positive attributes are associated with a significant increase in grain yield relative to wild-type controls that is consistent across years, environments, and elite germplasm backgrounds.

16.
Int J Mol Med ; 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31746364

RESUMO

The adaptive immune response mediated by T lymphocytes is a well­established factor in the pathogenesis of pulmonary inflammation. Changes in the expression of various connexins (Cxs) or disruption of connexin­mediated cellular communication in T lymphocytes contribute to inflammation or tissue remodeling. The aim of the present study was to investigate the potential therapeutic value of blocking Cxs in a monocrotaline (MCT)­induced pulmonary inflammation rat model. Carbenoxolone (CBX) was used to inhibit connexin­mediated cellular communication. An MCT rat model was established by intraperitoneal (i.p.) injection of a single dose of MCT (60 mg/kg), and CBX treatment (20 µg/kg/day, i.p.) was initiated on the day following MCT treatment for 28 days. Vehicle­treated male Sprague­Dawley rats were used as the negative control. The MCT rat model was evaluated by measuring the pulmonary artery flow acceleration time and right ventricular hypertrophy index (RVHI). Histopathological features of the lung tissues and pulmonary arteriolar remodeling were assessed. The proportions of T lymphocyte subtypes, Cx40/cx43 expression in the T cell subtypes and the cytokine levels in the plasma and the lung tissues were also analyzed. Pharmacological inhibition of Cxs using CBX attenuated MCT­induced right ventricular hypertrophy, pulmonary arteriolar remodeling, lung fibrosis and inflammatory cell infiltration by decreasing the RVHI, pulmonary arterial wall thickening, collagen deposition and pro­inflammatory cytokines production as well as CD3+ and CD4+ T cell accumulation in lung tissues of MCT­treated rats. Furthermore, flow cytometry analysis revealed that CBX may inhibit MCT­induced Cx40 and Cx43 expression in CD4+ and CD8+ T lymphocytes in lung tissues. The present study provides evidence that pharmacological inhibition of Cxs may attenuate MCT­induced pulmonary arteriolar remodeling and pulmonary inflammatory response, at least in part, by decreasing Cx expression. The results highlight the critical role of Cxs in T lymphocytes in the MCT­induced pulmonary inflammatory response and that targeting of Cxs may be a potential therapeutic method for treating pulmonary inflammatory diseases.

17.
Mol Med Rep ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31746411

RESUMO

Wilson disease (WD) is a rare autosomal recessive genetic disorder that causes abnormal copper metabolism, resulting in pathological accumulation of copper in the liver, brain and other organs. Mutations in the ATPase copper transporter 7B (ATP7B) gene, which encodes a membrane P­type adenosine triphosphatase, have been identified as being responsible for WD. The present study analyzed clinical data and collected DNA samples from a pediatric patient with WD and her healthy parents. Mutation screening for ATP7B was performed using direct sequencing, multiplex ligation­dependent probe amplification(MLPA), next­generation sequencing (NGS) and Sanger sequencing of the breakpoint junction sequence. The patient (age, 2.7 years) presented with early­onset hepatic disease. The present study identified compound heterozygous mutations of ATP7B, including a heterozygous mutation (p.Arg1,041Trp) and a novel heterozygous gross deletion of a 57,771 bp fragment (chr13: 52490972­52548742) (GRCh37) from partial exon2­ exon21 to external ATP7B sequence (15.833bp) in the patient. Analysis of the family members of the patient showed that the missense mutation and the gross deletion mutation were inherited from her mother and father, respectively. Microhomology and inverted repeat sequences, which may mediate the deletion mutation, were identified through sequence analysis on both sides of the breakpoints of this deletion. The present study provided additional information on the genotypic spectrum of the ATP7B gene, particularly with regard to early onset hepatic disease, as observed in the present patient with WD. The identification of the precise breakpoint junction sequence warrants further investigation of DNA break and recombination mechanisms. In detecting precise deletions, the NGS associated with Sanger sequencing of breakpoint junction sequence have been found to have more advantages than MLPA.

18.
Artigo em Inglês | MEDLINE | ID: mdl-31677814

RESUMO

The three-dimensional Bi2MoO6 mesoporous architectures constructed by nanosheets were synthesized through solvothermal method. The photocatalytic efficiency for tetracycline (TC) by the pristine Bi2MO6 and the BMO-450 (pristine Bi2MO6 was calcined at 450 °C) reached 73% and 90% after 150 min light irradiation, respectively. Compared to the photocatalytic efficiency of pristine Bi2MoO6, the BMO-450 exhibited much higher photocatalytic performance owing to the presence of oxygen vacancies obvious change the band structure of BMO-450. The X-ray photoelectron spectroscopy (XPS) revealed that the presence of oxygen vacancy in the BMO-450 crystal, which endowed it outstanding photocatalytic performance. Moreover, the BMO-450 possess high crystallization degree, mesoporous structure and the decreasing band gap. The excellent photocatalytic performance for the degradation of TC aqueous solutions, which demonstrate that the calcined Bi2MoO6 mesoporous spheres would have potential applications in the fields of treating wastewater.

19.
Appl Opt ; 58(27): 7359-7366, 2019 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-31674381

RESUMO

The phase-coding method has been widely used for 3D shape measurement, which uses sinusoidal phase-shifting patterns to recover the wrapped phase and the stair phase-coding patterns to determine the fringe order. However, due to random noises and image blurring, the fringe order is always misaligned with the wrapped phase, which will lead to fringe order errors. This paper presents an enhanced phase-coding method to address this misalignment problem by using half-period codewords, in which each codeword is aligned to the half-period of the sinusoidal patterns. Then, two complementary fringe orders with half-period dislocation can be calculated, which can effectively eliminate the fringe order errors. To extend the coding range of stair phase, this paper further develops a computational scheme based on the geometric constraint method. Simulations and experiments have been carried out, and their results confirm that the enhanced method can reliably recover the 3D shape of the measured objects.

20.
Artigo em Inglês | MEDLINE | ID: mdl-31727520

RESUMO

To understand the behavior of dimethyl phthalate (DMP) and realize the effect of DMP and its metabolite, monomethyl phthalate (MMP), on the conformation changes of bovine serum albumin (BSA), the interaction mechanisms of DMP and MMP with BSA were investigated by multispectroscopy and molecular docking. The results of the fluorescence quenching experiment showed that the fluorescence quenching of BSA by DMP and MMP was due to the formation of a complex though static quenching, which was also confirmed by time-resolved fluorescence measurements. The binding constants were 8.06 × 104 M-1 and 4.74 × 104 M-1 for DMP-BSA and MMP-BSA, respectively, and the number of binding sites were 1.20 (DMP) and 1.18 (MMP). The thermodynamic parameters showed different binding forces for DMP and MMP with BSA. The binding of DMP to BSA was driven mainly by hydrophobic interactions and hydrogen bonding, and MMP bound to BSA by van der Waals forces and hydrogen bonding, which were in accordance with the results from the molecular docking. The conformation and structural alterations of BSA upon DMP or MMP binding were studied by UV-vis spectroscopy, circular dichroism spectroscopy and synchronous fluorescence spectroscopy. The presence of metabolite did not change the quenching mechanism but decreased the binding affinity of DMP toward BSA as well as shortened the binding distance, which may be attributed to the competition between DMP and MMP for binding to BSA. This study revealed the combined effects of DMP and its metabolite on BSA at the molecular level.

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