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1.
Org Lett ; 22(5): 1836-1840, 2020 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-32049536

RESUMO

The aggregation-induced emission (AIE) mechanism of restriction of double-bond rotation (RDBR) was utilized to design an excellent solid emitter and sensor for the first time. Thus, cis-tetraphenylethylene (TPE) macrocycle diammoniums were synthesized and bound to a DNA chain by its two ammonium arms. The formed TPE dicycle at the cis position restricted the rotation of the double bond in both the ground and excited states, resulting in AIE enhancement, chiroptical performance enhancement, and sensing enhancement.

2.
Horm Cancer ; 10(4-6): 177-189, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31713780

RESUMO

In hepatocellular carcinoma (HCC), the hypoxic tumor microenvironment can drive enhance tumor malignancy and recurrence. The microRNA (miRNA) miR-196-5p has been shown to modulate the progression of several cancer types, but its roles in HCC remain uncertain. In the present report we observed significant miR-196-5p downregulation in HCC tissues and cells, and we found that the expression of this miRNA significantly impaired the proliferation and metastatic potential of HCC in vitro and in vivo. We identified high-mobility group AT-hook 2 (HMGA2) as a miR-196-5p target gene that was associated with the ability of miR-196-5p to modulate the progression of HCC. Expression of miR-196-5p and HMGA2 were correlated with the clinical characteristics and poor outcomes in patients with HCC. Finally, we found that hypoxic conditions were linked with reduced miR-196-5p expression in the context of HCC. Together these results highlight the role for miR-196-5p as an inhibitor of the proliferation and metastasis of HCC via the targeting of HMGA2, with this novel hypoxia/miR-196-5p/HMGA2 pathway serving as a potential target for future therapeutic intervention.

3.
Prehosp Disaster Med ; 34(6): 592-595, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31587678

RESUMO

INTRODUCTION: Severe trauma can lead to amputation of limbs. There is no golden standard or comprehensive evaluation indicator for amputation. It is difficult for the primary rescue organization to focus on the most essential indicators and to determine whether to perform amputation or take proper operation. PROBLEM: For medical staff in first-line medical teams for disaster relief or in a common primary hospital, what indicators should they focus on to keep the patient's limbs when they receive wounded patients with severe trauma? METHODS: A retrospective case-control study was performed based on the patients with severe trauma from January 2013 through December 2018 in the emergency department of Southwest Hospital (Shapingba District, Chongqing, China), a Level I trauma center. A total of 165 cases were divided into amputation group (n = 79) and non-amputation control group (n = 86), which had severe skin and muscle injury but without amputation. The causes of trauma and the special cases were analyzed. Binary logistic regression models were used to find the essential indicators for amputation. CONCLUSIONS: Neurovascular injury with delayed treatment was the most decisive indicator leading to amputation, and time phase was also important for limb salvage. Preliminary treatment of disaster victims and patients with severe trauma should focus on neurovascular status and timely delivery.

4.
Chin J Traumatol ; 20(5): 299-302, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28988729

RESUMO

Antivenom is the most effective method currently available for the treatment of poisonous snake bite. Allergic reactions to antivenom have been reported in the past. Here we shared a case of allergic reactions to antivenom in an old male patient who was bitten twice by the same snake (probably same one) at the same biting site within a month whereas the patient did not show any allergic disorder in the first bitten. Envenomations twice in a short period time by the same kind of snake are very rare. Physician should be alert to the occurrence of allergic reactions in treating this type of patients with antivenom. The skin allergy test has a certain value in predicting the allergic response before the second use of antivenom. Desensitization may reduce the incidence of allergic reactions, but this is insufficient. Rather than non-IgE-mediated immediate hypersensitivity, patients receiving the second treatment of antivenom may develop IgE-mediated immediate hypersensitivity. Once happened, the antivenom treatment should be stopped promptly and anti-allergy treatment should be given immediately.


Assuntos
Antivenenos/efeitos adversos , Hipersensibilidade/terapia , Mordeduras de Serpentes/terapia , Idoso , Animais , Humanos , Masculino
5.
Biophys J ; 112(10): 2173-2183, 2017 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-28538154

RESUMO

The interactions between Ca2+ ions and sphingomyelin play crucial roles in a wide range of cellular activities. However, little is known about the molecular details of the interactions at interfaces. In this work, we investigated the interactions between Ca2+ ions and egg sphingomyelin (ESM) Langmuir monolayers at the air/water interface by subwavenumber high-resolution broadband sum frequency generation vibrational spectroscopy (HR-BB-SFG-VS). We show that Ca2+ ions can induce ordering of the acyl chains in the ESM monolayer. An analysis of the one alkyl-chain-deuterated ESM revealed that the Ca2+ ions do not affect the N-linked saturated fatty acid chain, although they make the sphingosine backbone become ordered. Further analysis of the SFG-VS spectra shows that the interactions between ESM and Ca2+ ions make the orientation of the methyl group at the end of sphingosine backbone change from pointing downward to pointing upward. Moreover, a large blue shift of the phosphate group at the CaCl2 solution interface indicates, to our knowledge, new cation binding modes. Such binding causes the phosphate moiety to dehydrate, resulting in the conformation change of the phosphate moiety. Based on these results, we propose the molecular mechanism that Ca2+ ions can bind to the phosphate group and subsequently destroy the intramolecular hydrogen bond between the 3-hydroxyl group and the phosphate oxygen, which results in an ordering change of the sphingosine backbone. These findings illustrate the potential application of HR-BB-SFG-VS to investigate lipid-cation interactions and the calcium channel modulated by lipid domain formation through slight structural changes in the membrane lipid. It will also shed light on the interactions of complex molecules at surfaces and interfaces.


Assuntos
Cálcio/metabolismo , Esfingomielinas/metabolismo , Ar , Animais , Cloreto de Cálcio/química , Cátions Bivalentes/metabolismo , Galinhas , Proteínas do Ovo/metabolismo , Ligações de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Pressão , Soluções , Análise Espectral , Propriedades de Superfície , Vibração , Água/química
6.
BMC Pediatr ; 17(1): 90, 2017 03 28.
Artigo em Inglês | MEDLINE | ID: mdl-28351378

RESUMO

BACKGROUND: Previous studies have indicated edema may be involved in the pathophysiology following hypoxic-ischemic encephalopathy (HIE), and melatonin may exhibit neuro-protection against brain insults. However, little is known regarding the mechanisms that involve the protective effects of melatonin in the brain and peripheral tissues after HIE. The present study aimed to examine the effects of melatonin on multiple organs, and the expression of edema related proteins in a neonatal rat model of hypoxic-ischemic brain damage (HIBD). METHODS: One hundred ninety-two neonatal rats were randomly divided into three subgroups that underwent a sham surgery or HIBD. After the HIBD or sham-injury, the rats received an intraperitoneal injection of melatonin or an equal volume vehicle, respectively. We investigated the effects of melatonin on brain, kidney, and colon edema via histological examination and the expression of edema related proteins, including AQP-4, ZO-1 and occludin, via qPCR and western blot. RESULTS: Our data indicated (1) Melatonin reduced the histological injury in the brain and peripheral organs induced by HIBD as assessed via H-E staining and transmission electron microscopy. (2) Melatonin alleviated the HIBD-induced cerebral edema characterized by increased brain water content. (3) HIBD induced significant changes of edema related proteins, such as AQP-4, ZO-1 and occludin, and these changes were partially reversed by melatonin treatment. CONCLUSIONS: These findings provide substantial evidence that melatonin treatment has protective effects on the brain and peripheral organs after HIBD, and the edema related proteins, AQP4, ZO-1, and occludin, may indirectly contribute tothe mechanism of the edema protection by melatonin.


Assuntos
Edema Encefálico/prevenção & controle , Doenças do Colo/prevenção & controle , Edema/prevenção & controle , Hipóxia-Isquemia Encefálica/tratamento farmacológico , Nefropatias/prevenção & controle , Melatonina/uso terapêutico , Fármacos Neuroprotetores/uso terapêutico , Animais , Animais Recém-Nascidos , Biomarcadores/metabolismo , Western Blotting , Edema Encefálico/diagnóstico , Edema Encefálico/etiologia , Edema Encefálico/metabolismo , Doenças do Colo/diagnóstico , Doenças do Colo/etiologia , Doenças do Colo/metabolismo , Edema/diagnóstico , Edema/etiologia , Edema/metabolismo , Hipóxia-Isquemia Encefálica/complicações , Hipóxia-Isquemia Encefálica/fisiopatologia , Injeções Intraperitoneais , Nefropatias/diagnóstico , Nefropatias/etiologia , Nefropatias/metabolismo , Microscopia Eletrônica de Transmissão , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase em Tempo Real , Resultado do Tratamento
7.
Langmuir ; 33(15): 3694-3701, 2017 04 18.
Artigo em Inglês | MEDLINE | ID: mdl-28326789

RESUMO

Although the sophisticated Langmuir and Langmuir-Blodgett (LB) techniques facilitate the fabrication of uniform ultrathin monolayer and films, they are also revealed as powerful tools for the bottom-up construction of the nanostructures through the air/water interface. In this paper, unique nanoflowers or nanospheres were constructed based on the synthesized m-phthalic diamide-linked zinc bisporphyrinate tweezers using the Langmuir and LB techniques. It was found that the two tweezer-type zinc bisporphyrinates could form stable two-dimensional spreading films at the air/water interface, which could be subsequently transferred onto solid substrates using the vertical lifting method. Atomic force microscopy (AFM) revealed that at the initial spreading stage, the compound formed flat disklike domains and then hierarchically evolved into nanoflowers or nanospheres upon compressing the floating film. Such nanostructures have not been reported before and cannot be fabricated using the other self-assembly methods.

8.
Opt Express ; 24(22): A1349-A1359, 2016 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-27828521

RESUMO

p-type inorganic hole transport materials of Li, Cu-codoped NiOx films were deposited using a simple solution-based process. The as-prepared films were used as hole selective contacts for lead halide perovskite solar cell. An enhanced power conversion efficiency of 14.53% has been achieved due to the improved electrical conductivity and optical transmittance of the Li, Cu-codoped NiOx electrode interlayer.

9.
Guang Pu Xue Yu Guang Pu Fen Xi ; 36(1): 47-50, 2016 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-27228738

RESUMO

Achiral molecules with conjugated structures can form chiral supramolecules through interfacial self-assembly. These spontaneous symmetry breaking processes may help elucidate the origin of life and are thus of great importance. So far, the mechanism of interfaciam self-assembly has been discussed in detail. However, dynamics of the chiral assemblies was rarely investigated. In order to clarify whether the chiral structures are stable or dynamic, we employed second harmonic generation linear dichroism (SHG-LD) to investigate the supramolecular chirality of PARC18 at air/aqueous interface. It was shown that PARC18 formed chiral structures with stable chiral state at air/water interface. While at air/NaOH solution interface, the chiral state changed with time. In addition, on NaOH solution subphase, contributions of magnetic dipole to second harmonic signals were dominant. We suggest that this is due to isomerization of PARC18 molecules on NaOH solution subphase. As a result, the two chromophores coupled with each other and the magnetic dipole contribution was enhanced.

10.
Guang Pu Xue Yu Guang Pu Fen Xi ; 36(1): 287-91, 2016 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-27228784

RESUMO

According to the spectral absorption characteristics of polluting gases and fluorescence characteristics, a time-division multiplexing detection system is designed. Through this system we can detect Methane (CH4) and sulfur dioxide (SO2) by using spectral absorption method and the SO2 can be detected by using UV fluorescence method. The system consists of four parts: a combination of a light source which could be switched, the common optical path, the air chamber and the signal processing section. The spectral absorption characteristics and fluorescence characteristics are measured first. Then the experiment of detecting CH4 and SO2 through spectral absorption method and the experiment of detecting SO2 through UV fluorescence method are conducted, respectively. Through measuring characteristics of spectral absorption and fluorescence, we get excitation wavelengths of SO2 and CH4 measured by spectral absorption method at the absorption peak are 280 nm and 1.64 µm, respectively, and the optimal excitation wavelength of SO2 measured by UV fluorescence method is 220 nm. we acquire the linear relation between the concentration of CH4 and relative intensity and the linear relation between the concentration of SO2 and output voltage after conducting the experiment of spectral absorption method, and the linearity are 98.7%, 99.2% respectively. Through the experiment of UV fluorescence method we acquire that the relation between the concentration of SO2 and the voltage is linear, and the linearity is 99.5%. Research shows that the system is able to be applied to detect the polluted gas by absorption spectrum method and UV fluorescence method. Combing these two measurement methods decreases the costing and the volume, and this system can also be used to measure the other gases. Such system has a certain value of application.

11.
Zhongguo Dang Dai Er Ke Za Zhi ; 18(3): 270-6, 2016 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-26975828

RESUMO

OBJECTIVE: To study the changes of miRNA expression in the pineal gland of neonatal rats with hypoxic-ischemic brain damage (HIBD) and the possible roles of miRNA in the pathogenesis of circadian rhythm disturbance after HIBD. METHODS: Seven-day-old Sprague-Dawley (SD) rats were randomly divided into 2 groups: HIBD and sham-operated. HIBD was induced according to the Rice-Vannucci method. The pineal glands were obtained 24 hours after the HIBD event. The expression profiles of miRNAs were determined using GeneChip technigue and quantitative real-time PCR (RT-PCR). Then the miRNA which was highly expressed was selected. The expression levels of the chosen miRNA were detected in different tissues (lungs, intestines, stomach, kidneys, cerebral cortex, pineal gland). RT-PCR analysis was performed to measure the expression profiles of the chosen miRNA and the targeted gene Clock mRNA in the pineal gland at 0, 24, 48 and 72 hours after HIBD. RESULTS: miRNA-182 that met the criteria was selected by GeneChip and RT-PCR. miRNA-182 was highly expressed in the pineal gland. Compared with the sham-operated group, the expression of miRNA-182 was significantly up-regulated in the pineal gland at 24 and 48 hours after HIBD (P<0.05). Compared with the sham-operated group, Clock mRNA expression in the HIBD group increased at 0 hour after HIBD, decreased at 48 hours after HIBD and increased at 72 hours after HIBD (P<0.05). CONCLUSIONS: miRNA-182 may be involved in the pathogenesis of circadian rhythm disturbance after HIBD.


Assuntos
Proteínas CLOCK/genética , Ritmo Circadiano/fisiologia , Hipóxia-Isquemia Encefálica/fisiopatologia , MicroRNAs/fisiologia , Glândula Pineal/metabolismo , RNA Mensageiro/análise , Animais , Animais Recém-Nascidos , Feminino , Regulação da Expressão Gênica , Masculino , MicroRNAs/análise , Ratos , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase em Tempo Real
12.
J Insect Sci ; 152015.
Artigo em Inglês | MEDLINE | ID: mdl-26494776

RESUMO

Secondary reproductives develop primarily from nymphs. However, they have been rarely studied; in particular, the development of adultoid reproductives (AR) with floppy wings is still unclear. In this study, the change in juvenile hormone (JH) levels, vitellogenin gene expression, and oogenesis during the development of AR and brachypterous neotenic reproductives (BN) from the last instar nymphs of Reticulitermes labralis are investigated and compared. The results showed that the AR derived from the last instar nymphs by molting, and they were more similar to neotenic reproductives in morphology. In addition, the paired AR were not able to survive in the absence of workers. In R. labralis, the process of the last instar nymphs developing into AR and BN took an increase in JH level as a starting point. The JH level of the last instar nymphs molting into BN was approximately 1.5-fold higher than that of the AR. Additionally, The JHIII level of BN peaked on day 5, and that of AR peaked on day 10, which induced the onset of vitellogenesis in BN and AR, respectively. After molting, the vitellogenin gene expression levels of both BN and AR initially increased and then declined, and the expression levels in the BN were significantly higher than those in the AR. In addition, the oocytes of BN matured earlier than those of the AR, and the number of eggs laid by the BN was higher than the number laid by the AR. Our results demonstrate that, in R. labralis, the last instar nymphs can develop into AR, which are significantly different from BN in their development.


Assuntos
Isópteros/fisiologia , Hormônios Juvenis/metabolismo , Animais , Feminino , Isópteros/crescimento & desenvolvimento , Masculino , Muda , Ninfa/crescimento & desenvolvimento , Oócitos , Oviposição/fisiologia , Reprodução/fisiologia , Vitelogênese/fisiologia , Vitelogeninas/genética , Asas de Animais/crescimento & desenvolvimento , Asas de Animais/fisiologia
13.
BMC Genomics ; 16: 265, 2015 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-25886736

RESUMO

BACKGROUND: Panax notoginseng (Burk.) F.H. Chen is one of the most highly valued medicinal plants in the world. The major bioactive molecules are triterpene saponins, which are also known as ginsenosides. However, its large genome size has hindered the assembly of a draft genome by whole genome sequencing. Hence, genomic and transcriptomic details about P. notoginseng, especially its biosynthetic pathways and gene expression in different parts of the plant, have remained largely unknown until now. RESULTS: In this study, RNA sequencing of three different P. notoginseng tissues was performed using next generation DNA sequencing. After assembling the high quality sequencing reads into 107,340 unigenes, biochemical pathways were predicted and 9,908 unigenes were assigned to 135 KEGG pathways. Among them, 270 unigenes were identified to be involved in triterpene saponin biosynthesis. In addition, 350 and 342 unigenes were predicted to encode cytochrome P450s and glycosyltransferases, respectively, based on the annotation results, some of which encode enzymes responsible for the conversion of the triterpene saponin backbone into different ginsenosides. In particular, one unigene predominantly expressed in the root was annotated as CYP716A53v2, which probably participates in the formation of protopanaxatriol from protopanaxadiol in P. notoginseng. The differential expression of this gene was further confirmed by real-time PCR. CONCLUSIONS: We have established a global transcriptome dataset for P. notoginseng and provided additional genetic information for further genome-wide research and analyses. Candidate genes involved in ginsenoside biosynthesis, including putative cytochrome P450s and glycosyltransferases were obtained. The transcriptomes in different plant tissues also provide invaluable resources for future study of the differences in physiological processes and secondary metabolites in different parts of P. notoginseng.


Assuntos
Alcaloides/biossíntese , Ginsenosídeos/biossíntese , Panax notoginseng/metabolismo , Proteínas de Plantas/genética , Sistema Enzimático do Citocromo P-450/metabolismo , Flores/genética , Flores/metabolismo , Perfilação da Expressão Gênica/métodos , Glicosiltransferases/metabolismo , Panax notoginseng/genética , Folhas de Planta/genética , Folhas de Planta/metabolismo , Proteínas de Plantas/metabolismo , Raízes de Plantas/genética , Raízes de Plantas/metabolismo , Sapogeninas/metabolismo
14.
Gene ; 547(1): 169-74, 2014 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-24952136

RESUMO

Pax6 functions as a pleiotropic regulator in eye development and neurogenesis. Its splice variant Pax6 5a has been cloned in many vertebrate species including human and mouse, but never in rat. This study focused on the cloning and characterization of the Pax6 5a orthologous splicing variant in rat. It was cloned from Sprague-Dawley rats 10 days post coitum (E10) by RT-PCR and was sequenced for comparison with Pax6 sequences in the GenBank by BLAST. The rat Pax6 5a was revealed to contain an additional 42 bp insertion at the paired domain. At the nucleotide level, the rat Pax6 5a coding sequence (1,311 bp) had a higher degree of homology to the mouse (96% identical) than to the human (93% identical) sequence. At the amino acid (aa) level, rat PAX6 5a shares 99.8% identity with the mouse sequence and 99.5% with the human sequence. The splice variant is preferentially expressed in the rat E10 embryonic headfolds and not in the trunk of neurula. Its effects on the proliferation of rat mesenchymal stem cells (rMSCs) were preliminarily evaluated by the MTT assay. Both pLEGFP-Pax6 5a-transfected cells and pLEGFP-Pax6-transfected cells exhibited a similar growth curve (P>0.05), suggesting that the Pax6 5a has a similar effect on the proliferation of rMSCs as Pax6.


Assuntos
Proteínas do Olho/genética , Proteínas de Homeodomínio/genética , Fator de Transcrição PAX5/genética , Fatores de Transcrição Box Pareados/genética , Processamento de RNA , Proteínas Repressoras/genética , Animais , Sequência de Bases , Células Cultivadas , Clonagem Molecular , Primers do DNA , Células HEK293 , Humanos , Células-Tronco Mesenquimais/metabolismo , Camundongos , Células NIH 3T3 , Fator de Transcrição PAX6 , Ratos , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase Via Transcriptase Reversa
15.
Med Sci Monit ; 19: 194-201, 2013 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-23492458

RESUMO

BACKGROUND: This aim of this study was to investigate the effects of one-side cervical sympathetic block on early inflammatory response in severe trauma patients. MATERIAL AND METHODS: Thirty severe trauma patients with injury severity score (ISS) of 16 to 25 were randomly divided into treatment and control groups (n=15 each). Patients in the treatment group underwent a right-side stellate ganglion block (SGB) using 8 mL 0.75% ropivacaine for 4 times, with the first injection within 12 hr of admission and the other 3 injections were 12 hr, 24 hr and 48 hr later. The same procedures were performed for the control group except that normal saline was injected instead of ropivacaine. Blood was collected before injection and at 6 hr, 24 hr, and 72 hr after the first SGB for serum interleukin (IL)-1beta, IL-4, IL-6, IL-10 and TNF-alpha measurement. RESULTS: The concentrations of IL-1beta, IL-6, and TNF-alpha between 24 hr to 72 hr after SGB were all significantly lower than those in the control group (all P values <0.01). However, there was no significant difference in the concentrations of anti-inflammatory IL-4 and IL-10 between treatment and control groups. There was no obvious impact of SGB on breathing and circulation except for a slower heart rate 10 to 50 min after injection (P<0.01). CONCLUSIONS: SGB regulates early inflammatory response through inhibition of the proinflammatory cytokines IL-1beta, IL-6, and TNF-alpha during severe trauma. SGB has no impact on the levels of anti-inflammatory cytokines IL-4 and IL-10.


Assuntos
Bloqueio Nervoso Autônomo , Vértebras Cervicais/patologia , Inflamação/complicações , Inflamação/patologia , Ferimentos e Lesões/complicações , Ferimentos e Lesões/terapia , Adulto , Amidas/administração & dosagem , Amidas/farmacologia , Estudos de Casos e Controles , Vértebras Cervicais/efeitos dos fármacos , Vértebras Cervicais/fisiopatologia , Citocinas/sangue , Feminino , Humanos , Inflamação/sangue , Inflamação/fisiopatologia , Mediadores da Inflamação/sangue , Masculino , Pessoa de Meia-Idade , Respiração/efeitos dos fármacos , Ropivacaina , Gânglio Estrelado/efeitos dos fármacos , Ferimentos e Lesões/sangue , Ferimentos e Lesões/fisiopatologia , Adulto Jovem
16.
Exp Toxicol Pathol ; 64(6): 543-7, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21159497

RESUMO

AIM: Excessive production of inflammatory mediators during invasive infection plays a key role in the pathogenesis of sepsis. In an attempt to improve survival of patients with this lethal syndrome, agents were developed to selectively inhibit mediators in this inflammatory response. Ulinastatin (UTI), a human protease inhibitor, inhibits the enhanced production of pro-inflammatory molecules. However, it is unknown if Ulinastatin treatment could result in protective effects for sepsis. The aim of this study was to investigate the role of Ulinastatin on septic rats. METHODS: Sixty male Wistar rats were divided into six groups, 10 of each: sham-operation plus PBS (5 ml), cecal ligation and puncture (CLP) plus PBS (5 ml), CLP plus UTI (5000 U/kg), CLP plus UTI (10,000 U/kg), CLP plus UTI (20,000 U/kg) and sham-operation plus UTI (10,000 U/kg). Rats in the UTI groups after CLP operation were treated with Ulinastatin by intraperitoneal injection at different doses and then compared with untreated sepsis control animals. RESULTS: The intestinal concentrations of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), and interleukin-13 (IL-13) were significantly higher in septic rats than those in normal rats. Ulinastatin administration effectively suppressed the levels of TNF-α and IL-6, whereas it markedly enhanced the levels of IL-10 and IL-13. CONCLUSION: Ulinastatin may possess a protective role in the septic process by inhibiting TNF-α and IL-6, and augmenting IL-10 and IL-13 concentrations in intestine of septic rats.


Assuntos
Anti-Inflamatórios/farmacologia , Glicoproteínas/farmacologia , Sepse/prevenção & controle , Inibidores da Tripsina/farmacologia , Animais , Modelos Animais de Doenças , Ensaio de Imunoadsorção Enzimática , Interleucina-1/biossíntese , Interleucina-10/biossíntese , Interleucina-6/biossíntese , Masculino , Ratos , Ratos Wistar , Sepse/imunologia , Fator de Necrose Tumoral alfa/biossíntese
17.
Acta Cardiol ; 65(4): 431-9, 2010 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-20821936

RESUMO

OBJECTIVE: The present study was designed to explore the roles of MMP-2/TIMP-2 in cardiac fibrosis and to study the effects of benazepril, an angiotensin-converting enzyme inhibitor (ACEI) on cardiac remodelling in streptozotocin(STZ)-induced diabetic rats. METHODS AND RESULTS: Male Wistar rats were randomly divided into three groups: a normal control group (NC), a diabetes mellitus-untreated group (DM) and a diabetes mellitus benazepril-treated group (DB). Diabetes mellitus was induced in the DM and DB groups by intraperitoneal injection of streptozotocin (60 mg/kg). DB rats were treated with benazepril 10 mg/kg/day for 12 weeks by remedial perfusing of the stomach. In the DM group, compared with the NC group, the gene and protein expression of MMP-2 decreased while the TIMP-2 gene and protein expression increased in heart tissues, along with a markedly cardiac collagen deposition.All the above changes were attenuated by benazepril treatment in the DB group. CONCLUSIONS: The imbalance of MMP-2 and TIMP-2 expressions in heart tissues might participate in interstitial fibrosis in diabetic myocardiopathy. Benazepril may ameliorate cardiac fibrosis partly by regulating the MMP-2/TIMP-2 system.


Assuntos
Inibidores da Enzima Conversora de Angiotensina/farmacologia , Benzazepinas/farmacologia , Diabetes Mellitus Experimental/tratamento farmacológico , Miocárdio/patologia , Análise de Variância , Animais , Glicemia/metabolismo , Peso Corporal , Colágeno/metabolismo , Fibrose/tratamento farmacológico , Técnicas Imunoenzimáticas , Masculino , Metaloproteinase 2 da Matriz/metabolismo , Tamanho do Órgão , Distribuição Aleatória , Ratos , Ratos Wistar , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Coloração e Rotulagem , Estreptozocina , Inibidor Tecidual de Metaloproteinase-2/metabolismo
18.
Langmuir ; 25(17): 10116-20, 2009 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-19456102

RESUMO

Ionic liquid (IL)-reconstituted acrylic acid (AA)-functionalized cellulose microparticles were successfully prepared by a water-in-oil suspension technique preliminary modification with AA in homogeneous condition. Cellulose was fully dissolved in 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) IL, and subsequently was grafted homogeneously with AA and N,N'-methylenebisacrylamide (N,N'-MBA) initiated with ammonium persulfate. The grafted cellulose was spheroidized using white silicone oil as the dispersion medium and Span 80 as a dispersant and then reconstituted from [Bmim]Cl. Reaction conditions were optimized to obtain microparticles with both the highest possible grafting efficiency and most uniform bead sizes. Fourier transform infrared spectroscopy, scanning electron microscopy, and an optical microscope were employed to provide structural information for the functionalized IL-reconstituted cellulose microparticles. These microparticles were shown to behave as good sorbents for Cu(II), Ni(II), Fe(III) ions.

19.
Zhong Yao Cai ; 32(12): 1867-70, 2009 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-20432905

RESUMO

OBJECTIVE: To investigate the effects of tetrandrine on nuclear factor-kappaB (NF-kappaB) activity and proliferation of Diabetes Mellitus (DM) rats vascular smooth muscle cells (VSMCs). METHODS: DM models were established,the culture of DM and normal VSMCs were done by modified tissue-piece incoculztion; Inhibitory effect of Tetrandrine was determined by cell counting kit (CCK-8). Immunofluorescence laser confocal microscopy was used to estimate the effect of NF-kappaB in DM VSMCs with Tet. RESULTS: Tetrandrine inhibited the proliferation of DM and normal VSMCs,the IC50 value was (11.35 +/- 1.24) micromol/L and (28.01 +/- 4.35) micromol/L, respectively. Furthermore, Tetrandrine strongly inhibited the activation of NF-kappaB in DM VSMCs and reduced the nuclear translocation of NF-kappaB. CONCLUSION: Tetrandrine inhibits the proliferation of DM VSMCs more effectively than that of normal, the mechanism may be related to reducing NF-kappaB nuclear translocation.


Assuntos
Benzilisoquinolinas/farmacologia , Bloqueadores dos Canais de Cálcio/farmacologia , Proliferação de Células/efeitos dos fármacos , Diabetes Mellitus Experimental/patologia , Músculo Liso Vascular/efeitos dos fármacos , Stephania tetrandra/química , Animais , Células Cultivadas , Diabetes Mellitus Experimental/metabolismo , Masculino , Músculo Liso Vascular/metabolismo , NF-kappa B/efeitos dos fármacos , NF-kappa B/metabolismo , Plantas Medicinais/química , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Estreptozocina
20.
Acta Cardiol ; 62(5): 485-91, 2007 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-17982970

RESUMO

OBJECTIVE: The present study was designed to examine the changes of MMP-2/TIMP-2 in the hearts of streptozotocin-induced diabetic rats and gain insight into their roles in extracellular matrix (ECM) remodelling on an experimental animal model of diabetic cardiomyopathy. METHODS AND RESULTS: Sixteen male Wistar rats were randomly divided into two groups: normal control and diabetic rats. Diabetes mellitus was induced in rats by streptozotocin injection. All rats were fed with standard chow and water ad libitum for 4 weeks. At 4 weeks diabetic rats were associated with a lower body weight (BW) and heart weight (HW) but a higher HW/BW. In the diabetic group, serum MMP-2 level had a tendency to increase but not significantly, while serum TIMP-2 significantly increased. Both the activity and expression of MMP-2 weakened in the hearts of diabetic rats. TIMP-2 gene expression in myocardium enhanced significantly. TIMP-2 protein level in diabetic heart was strengthened slightly but not significantly. VG staining showed a marked deposition of collagen in the diabetic group. Multivariate analysis revealed that total collagen content correlated negatively with the activity and gene expression of MMP-2 in the myocardium, and correlated positively with TIMP-1 mRNA expression. CONCLUSIONS: The decrease in MMP-2 activity and expression and increase in TIMP-2 gene expression in the myocardium of diabetic rats may lead to impairment of collagen degradation and contribute to the matrix deposition in diabetic myocardiopathy. The correlation between the serum level and cardiac expression of TIMP-2 in diabetic rats suggested that serum TIMP-2 level may be a viable marker for early diagnosis of diabetic myocardiopathy.


Assuntos
Cardiomiopatias/fisiopatologia , Complicações do Diabetes , Diabetes Mellitus Experimental , Diabetes Mellitus/fisiopatologia , Matriz Extracelular , Metaloproteinase 2 da Matriz/fisiologia , Inibidor Tecidual de Metaloproteinase-2/fisiologia , Animais , Cardiomiopatias/etiologia , Expressão Gênica , Imuno-Histoquímica , Modelos Animais , RNA Mensageiro , Ratos , Ratos Wistar
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