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1.
Behav Brain Res ; : 113621, 2021 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-34624424

RESUMO

Sleep is essential for important physiological functions. Impairment of learning and memory function caused by lack of sleep is a common physiological phenomenon of which underlying changes in synaptic plasticity in the hippocampus are not well understood. The possible different effects of sleep deprivation (SD) lasting for various durations on learning and memory function and hippocampal synaptic plasticity are still not completely clear. In this study, we used a modified multiple platform method (MMPM) to induce rapid eye movement SD (REM SD), lasting for 24h, 48h, and 72h, separately. The novel place recognition (NPR) and novel object recognition (NOR) tasks were used to test the novelty-related object recognition memory (ORM) and object location memory (OLM) of mice. Then, hippocampal synaptic plasticity was evaluated after all behavioural experiments. The results showed that REM SD played a key role in OLM but not in ORM. Specifically, 24h REM SD improved novelty-related OLM, accompanied by a significantly increased hippocampal synaptic plasticity, including gain of dendritic spines, increased expression of hippocampal GluA1, and enhanced long-term potentiation (LTP), whereas 48h REM SD showed no effect on OLM or the hippocampal synaptic plasticity mentioned above; however, 72h REM SD impaired novelty-related OLM and weakened hippocampal synaptic plasticity, including serious loss of dendritic spines, decreased expression of hippocampal GluA1, and significantly attenuated LTP. Our results suggest that REM SD of various durations has different effects on both novelty-related OLM and hippocampal synaptic plasticity.

2.
Nat Commun ; 12(1): 5231, 2021 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-34471132

RESUMO

Biological sodium channels ferry sodium ions across the lipid membrane while rejecting potassium ions and other metal ions. Realizing such ion selectivity in an artificial solid-state ionic device will enable new separation technologies but remains highly challenging. In this work, we report an artificial sodium-selective ionic device, built on synthesized porous crown-ether crystals which consist of densely packed 0.26-nm-wide pores. The Na+ selectivity of the artificial sodium-selective ionic device reached 15 against K + , which is comparable to the biological counterpart, 523 against Ca2 + , which is nearly two orders of magnitude higher than the biological one, and 1128 against Mg2 + . The selectivity may arise from the size effect and molecular recognition effect. This work may contribute to the understanding of the structure-performance relationship of ion selective nanopores.

3.
Water Sci Technol ; 84(5): 1079-1089, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34534107

RESUMO

Dewatered sewage sludge (DSS) and anaerobically digested sludge (ADS) were pyrolyzed at 550 °C to investigate the characteristics of derived biochar and evaluate the risk of heavy metals (Cr, Ni, Cu, As, Cd, and Pb). The results showed that the pH value of the biochar derived from DSS (DSS-C) was slightly lower than that of the biochar derived from ADS (ADS-C), while DSS-C presented relatively higher specific surface area and total pore volume. DSS-C also showed higher H/C and lower O/C ratios than ADS-C, indicating a higher aromatic condensation and a lower polarity. Total concentrations of Cr, Ni, Cu, As, Cd, and Pb in DSS and ADS increased significantly after pyrolysis owing to the thermal decomposition of organic matter in the sludge, with corresponding rise of the Nemerow pollution index (NPI) of the biochars compared with the raw sludge. In addition, the sequential extraction procedure (BCR) analysis revealed that the pyrolysis process promoted the transformation of heavy metals from bio-available fractions to stable fractions. The potential environmental risk of heavy metals decreased from moderate and extremely high levels in the DSS and ADS to low risk and moderate levels in DSS-C and ADS-C after pyrolysis, respectively.


Assuntos
Metais Pesados , Esgotos , Carvão Vegetal , Medição de Risco
4.
Food Funct ; 12(20): 9994-10007, 2021 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-34505615

RESUMO

This study evaluated the hypoglycemic effect of Auricularia auricula polysaccharides (AAPs) on streptozotocin-induced type 2 diabetes mellitus (T2DM) mice using metabolomic analysis. The results of fasting blood glucose, oral glucose tolerance test, fasting serum insulin level, Homeostatic Model Assessment of Insulin Resistance index, TC, TG, HDL-C, LDL-C, and histopathological observation demonstrated that 200 mg per kg body weight per day AAP led to significant hypoglycemic activities. The metabolic profile of the mice was significantly changed after AAP intervention. 45 differential metabolites were screened as biomarkers for AAP adjuvant treatment, and AAPs' effects on the metabolism of amino acids, unsaturated fatty acids, bile acids, and glycerophospholipids were analyzed. Thus, the current results elucidated the metabolic pathway of AAPs for T2DM alleviation and provided guidance for functional food adjuvant development for T2DM treatment.

5.
Ecotoxicol Environ Saf ; 224: 112631, 2021 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-34416634

RESUMO

In view of their vast global usage in both consumer products and industrial processes, environmental emission and fates of siloxanes have become concerned issue. This review summarized the research progress, especially in the last decade, on production/consumption data, toxicities, analysis methods, environmental distribution, migration and degradation/transformation of both dimethylsiloxanes and modified siloxanes in atmospheric, aquatic and terrestrial compartments from various areas (especially in China). In spite of their fast degradation (hydrolysis and hydroxylation, etc) in various matrices (except sediment), dimethylsiloxane oligomers have been found in various environmental matrices from many countries due to their constant usage and emission. Moreover, recent literatures have paid attention to behaviors of dimethylsiloxanes in industrial areas, e.g., their higher residual levels compared with residential areas and unique transformed products (such as halogenated products) arose from special industrial production scenarios. Meanwhile, although most prior studies focused on dimethylsiloxanes, identification of modified-siloxanes with other functional groups in environment have been beginning to attract the attention of scientists. Furthermore, related literatures indicated that compared with dimethylsiloxanes, both halogenated-dimethylsiloxanes and modified methylsiloxanes (phenylsiloxanes and trifluoropropylsiloxanes) could have stronger persistence due to their weaker volatilization and degradation, especially in terrestrial matrices.

6.
Sci Total Environ ; 796: 149039, 2021 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-34328900

RESUMO

Cadmium (Cd) is one of the most harmful heavy metals due to its persistence and bioaccumulation through the food chains, posing health risks to human. Oysters can bioaccumulate and tolerate high concentrations of Cd, providing a great model for studying molecular mechanism of Cd detoxification. In a previous study, we identified two CYP genes, CYP17A1-like and CYP2C50, that were potentially involved in Cd detoxification in the Pacific oyster, Crassostrea gigas. In this work, we performed further investigations on their physiological roles in Cd detoxification through RNA interference (RNAi). After injection of double-stranded RNA (dsRNA) into the adductor muscle of oysters followed by Cd exposure for 7 days, we observed that the expressions of CYP17A1-like and CYP2C50 in interference group were significantly suppressed on day 3 compared with control group injected with PBS. Moreover, the mortality rate and Cd content in the CYP17A1-like dsRNA interference group (dsCYP17A1-like) was significantly higher than those of the control on day 3. Furthermore, the activities of antioxidant enzymes, including SOD, CAT, GST, were significantly increased in dsCYP17A1-like group, while were not changed in dsCYP2C50 group. More significant tissue damage was observed in gill and digestive gland of oysters in RNAi group than control group, demonstrating the critical role of CYP17A1-like in Cd detoxification. Dual luciferase reporter assay revealed three core regulatory elements of MTF-1 within promoter region of CYP17A1-like, suggesting the potential transcriptional regulation of CYP17A1-like by MTF-1 in oysters. This work demonstrated a critical role of CYP17A1-like in Cd detoxification in C. gigas and provided a new perspective toward unravelling detoxification mechanisms of bivalves under heavy metal stress.


Assuntos
Crassostrea , Poluentes Químicos da Água , Animais , Cádmio/metabolismo , Cádmio/toxicidade , Crassostrea/genética , Crassostrea/metabolismo , Regulação da Expressão Gênica , Brânquias/metabolismo , Poluentes Químicos da Água/toxicidade
7.
Genet Test Mol Biomarkers ; 25(7): 478-485, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34280007

RESUMO

Background: Multiple osteochondroma (MO), an autosomal dominant genetic disease, is caused by heterozygous mutations in the EXT1 and EXT2 genes. Approximately 80% of pathogenic mutations are nonsense/missense mutations, small indels, and splicing mutations. Splicing mutations, particularly at the 3' and 5' splice sites, disrupt normal mRNA processing and cause exon skipping or aberrant splicing, ultimately resulting in protein truncation and loss of function. Methods: Polymerase chain reaction (PCR) and Sanger sequencing were applied to detect subtle mutations in a Chinese family with MO, the pathogenicity of a splicing variant was predicted by bioinformatics and further verified using a minigene splicing assay. Results: A novel and heterozygous splicing mutation, c.626 + 2_626 + 5delTAGG, was identified in the EXT2 gene of the proband and the father by PCR and Sanger sequencing, whereas the unaffected mother and brother had wild-type alleles at the same site. Bioinformatics predicted that the 5' splicing site of exon 3 in the EXT2 gene was destroyed due to this mutation. A hybrid minigene splicing assay (HMSA) indicated that the mutation disturbed the normal splicing of the EXT2 gene mRNA and led to a deletion of 79 bp at the 5' end of exon 3, which resulted in aberrant splicing of exon 3 and introduced an earlier stop codon in the EXT2 gene. Conclusion: A novel splicing mutation was identified that produced the MO phenotype through aberrant splicing in a Chinese family. This observation, expands our knowledge of the spectrum of molecular pathogenic mechanisms leading to aberrant mRNA splicing.


Assuntos
Exostose Múltipla Hereditária/genética , N-Acetilglucosaminiltransferases/genética , Adulto , Alelos , Grupo com Ancestrais do Continente Asiático/genética , China , Éxons/genética , Exostose Múltipla Hereditária/metabolismo , Feminino , Humanos , Íntrons/genética , Masculino , Mutação/genética , Mutação de Sentido Incorreto , N-Acetilglucosaminiltransferases/metabolismo , Linhagem , Fenótipo , Sítios de Splice de RNA/genética , Splicing de RNA/genética
8.
Sci Rep ; 11(1): 14879, 2021 07 21.
Artigo em Inglês | MEDLINE | ID: mdl-34290308

RESUMO

The removal and chemical speciation changes of heavy metals in the sewage sludge during the single bioleaching and combined bioleaching/Fenton-like processes were compared in this study. The improvement in the dewaterability of the treated sludge was also investigated. The single bioleaching led to a removal of Zn, Cu, Cd, Cr, Mn, Ni, As and Pb of 67.28%, 50.78%, 64.86%, 6.32%, 56.15%, 49.83%, 20.78% and 10.52% in 10 days, respectively. The chemical speciation analysis showed that the solubilization of heavy metals in mobile forms (exchangeable/acid soluble and reducible forms) and oxidizable form was the main reason for their removal. Subsequent Fenton-like treatment was carried out at different bioleaching stages when the bioleached sludge dropped to certain pH values (4.5, 4.0 and 3.0), by adding H2O2 at different dosages. The highest removal ratio of Zn, Cu, Cd, Cr, Mn and Ni could reach 75.53%, 52.17%, 71.91%, 11.63%, 66.29% and 65.19% after combined bioleaching/Fenton-like process, respectively, with appropriate pH and H2O2 dosages in less than 6 days. The solubilization efficiencies of these heavy metals in mobile forms were further improved by Fenton-like treatment. The removal efficiencies of As and Pb decreased due to their transformation into insoluble forms (mostly residual fraction) after Fenton treatment. The capillary suction times (CST) of the raw sludge (98.7 s) decreased by 79.43% after bioleaching and 87.44% after combined process, respectively.

9.
J Phys Chem Lett ; : 7357-7364, 2021 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-34324348

RESUMO

Guest/host phosphorescence materials have attracted much attention; traditionally, researchers have focused on the influence of the electronic properties and energy levels of the molecules on the phosphorescence activities. However, the effects of the morphology on the phosphorescence properties are ignored. Herein, three isoquinoline guests with different aliphatic rings and three hosts are selected to construct guest/host materials. Experimental results confirm that the guests are dispersed in the host in the form of clusters. More importantly, the morphologies of the guest/host directly affect the phosphorescence properties. In these systems, the guests have strong intermolecular interactions, which are beneficial to stabilize the triplet excitons; meanwhile, the hosts should have weak intermolecular interactions with easily changed morphology to accept the guest clusters, which synergistically ensure that the doped materials have excellent RTP properties. This is the first work focusing on the effect of molecular morphology on the phosphorescence characteristics of guest/host systems.

10.
Chem Commun (Camb) ; 57(58): 7152-7155, 2021 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-34184013

RESUMO

Glass micropipettes are easy to fabricate, have excellent flexibility and stable properties. HKUST-1 and MIL-68(In) are in situ grown in the tip of a micropipette to construct porous nanochannels. After absorbing H2S, the MIL-68(In)-based nanochannel shows effective metal ion responsiveness for Hg2+-detection.


Assuntos
Sulfeto de Hidrogênio/análise , Estruturas Metalorgânicas/química , Adsorção , Vidro/química , Sulfeto de Hidrogênio/isolamento & purificação , Nanoestruturas/química , Porosidade
11.
Front Immunol ; 12: 586078, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34177881

RESUMO

Allergic diseases are significant diseases that affect many patients worldwide. In the past few decades, the incidence of allergic diseases has increased significantly due to environmental changes and social development, which has posed a substantial public health burden and even led to premature death. The understanding of the mechanism underlying allergic diseases has been substantially advanced, and the occurrence of allergic diseases and changes in the immune system state are known to be correlated. With the identification and in-depth understanding of innate lymphoid cells, researchers have gradually revealed that type 2 innate lymphoid cells (ILC2s) play important roles in many allergic diseases. However, our current studies of ILC2s are limited, and their status in allergic diseases remains unclear. This article provides an overview of the common phenotypes and activation pathways of ILC2s in different allergic diseases as well as potential research directions to improve the understanding of their roles in different allergic diseases and ultimately find new treatments for these diseases.


Assuntos
Dermatite Atópica/imunologia , Hipersensibilidade Alimentar/imunologia , Rinite Alérgica/imunologia , Células Th2/imunologia , Alérgenos/imunologia , Animais , Citocinas/metabolismo , Humanos , Imunidade Inata , Fenótipo
12.
Int J Mol Sci ; 22(10)2021 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-34067660

RESUMO

G-protein-coupled receptors (GPCRs) are known to play central roles in the physiology of many organisms. Members of this seven α-helical transmembrane protein family transduce the extracellular signals and regulate intracellular second messengers through coupling to heterotrimeric G-proteins, adenylate cyclase, cAMPs, and protein kinases. As a result of the critical function of GPCRs in cell physiology and biochemistry, they not only play important roles in cell biology and the medicines used to treat a wide range of human diseases but also in insects' physiological functions. Recent studies have revealed the expression and function of GPCRs in insecticide resistance, improving our understanding of the molecular complexes governing the development of insecticide resistance. This article focuses on the review of G-protein coupled receptor (GPCR) signaling pathways in insect physiology, including insects' reproduction, growth and development, stress responses, feeding, behaviors, and other physiological processes. Hormones and polypeptides that are involved in insect GPCR regulatory pathways are reviewed. The review also gives a brief introduction of GPCR pathways in organisms in general. At the end of the review, it provides the recent studies on the function of GPCRs in the development of insecticide resistance, focusing in particular on our current knowledge of the expression and function of GPCRs and their downstream regulation pathways and their roles in insecticide resistance and the regulation of resistance P450 gene expression. The latest insights into the exciting technological advances and new techniques for gene expression and functional characterization of the GPCRs in insects are provided.


Assuntos
Resistência a Inseticidas/genética , Receptores Acoplados a Proteínas G/metabolismo , Receptores Acoplados a Proteínas G/fisiologia , Animais , Sistema Enzimático do Citocromo P-450/genética , Sistema Enzimático do Citocromo P-450/metabolismo , Insetos/genética , Insetos/metabolismo , Transdução de Sinais/fisiologia
13.
Molecules ; 26(10)2021 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-34069969

RESUMO

G-protein coupled receptors (GPCRs) play important roles in cell biology and insects' physiological processes, toxicological response and the development of insecticide resistance. New information on genome sequences, proteomic and transcriptome analysis and expression patterns of GPCRs in organs such as the central nervous system in different organisms has shown the importance of these signaling regulatory GPCRs and their impact on vital cell functions. Our growing understanding of the role played by GPCRs at the cellular, genome, transcriptome and tissue levels is now being utilized to develop new targets that will sidestep many of the problems currently hindering human disease control and insect pest management. This article reviews recent work on the expression and function of GPCRs in insects, focusing on the molecular complexes governing the insect physiology and development of insecticide resistance and examining the genome information for GPCRs in two medically important insects, mosquitoes and house flies, and their orthologs in the model insect species Drosophila melanogaster. The tissue specific distribution and expression of the insect GPCRs is discussed, along with fresh insights into practical aspects of insect physiology and toxicology that could be fundamental for efforts to develop new, more effective, strategies for pest control and resistance management.


Assuntos
Insetos/efeitos dos fármacos , Insetos/metabolismo , Inseticidas/toxicidade , Receptores Acoplados a Proteínas G/metabolismo , Animais , Regulação da Expressão Gênica/efeitos dos fármacos , Insetos/genética , Resistência a Inseticidas/genética , Especificidade de Órgãos/efeitos dos fármacos , Especificidade de Órgãos/genética
14.
Int J Mol Sci ; 22(9)2021 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-34063651

RESUMO

Salt stress is the second most important abiotic stress factor in the world, which seriously affects crop growth, development and grain production. In this study, we performed the first integrated physiological and endoplasmic reticulum (ER) proteome analysis of wheat seedling leaves under salt stress using a label-free-based quantitative proteomic approach. Salt stress caused significant decrease in seedling height, root length, relative water content and chlorophyll content of wheat seedling leaves, indicating that wheat seedling growth was significantly inhibited under salt stress. The ER proteome analysis identified 233 ER-localized differentially accumulated proteins (DAPs) in response to salt stress, including 202 upregulated and 31 downregulated proteins. The upregulated proteins were mainly involved in the oxidation-reduction process, transmembrane transport, the carboxylic acid metabolic process, stress response, the arbohydrate metabolic process and proteolysis, while the downregulated proteins mainly participated in the metabolic process, biological regulation and the cellular process. In particular, salt stress induced significant upregulation of protein disulfide isomerase-like proteins and heat shock proteins and significant downregulation of ribosomal protein abundance. Further transcript expression analysis revealed that half of the detected DAP genes showed a consistent pattern with their protein levels under salt stress. A putative metabolic pathway of ER subproteome of wheat seedling leaves in response to salt stress was proposed, which reveals the potential roles of wheat ER proteome in salt stress response and defense.


Assuntos
Proteínas de Plantas/genética , Proteoma/genética , Estresse Salino/genética , Triticum/genética , Mecanismos de Defesa , Grão Comestível/genética , Grão Comestível/crescimento & desenvolvimento , Retículo Endoplasmático/genética , Folhas de Planta/genética , Folhas de Planta/crescimento & desenvolvimento , Plântula/genética , Estresse Fisiológico/genética , Triticum/crescimento & desenvolvimento
15.
Mol Immunol ; 137: 1-10, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34175710

RESUMO

Epidemiological studies have suggested that fine particulate matter (PM2.5) and asthma have been independently associated with pulmonary fibrosis but rarely studied together. Furthermore, it is unknown whether airway fibrosis in asthma is more attributable to water-soluble ions of PM2.5. Our current study was to explore the potential mechanism of PM2.5 and water-soluble components on airway fibrosis in ovalbumin (OVA)-sensitized asthmatic rats. Rats were intratracheally instilled with PM2.5 and water-soluble components every 3 days for 4 times or 8 times. Histopathological examination demonstrated that lung inflammatory and airway fibrosis were induced after PM2.5 and water-soluble components exposure. Meanwhile, PM2.5, in particular water-soluble extracts, increased expression of collagen 1 (COL-1), connective tissue growth factor (CTGF), interleukin-6 (IL-6), transforming growth factor-ß1 (TGF-ß1), Smad family member 3 (Smad3), and p-Smad3, whereas decreased secretion of heme oxygenase-1 (HO-1). However, pretreating asthmatic rats with SB432542, the inhibitor of TGF-ß1, and SIS3 HCl, the antagonist of Smad3, both reversed the activation of airway fibrosis induced by water-soluble extracts. Therefore, TGF-ß1/Smad3 signaling pathway may be responsible for the pathological process of airway fibrosis in asthmatic rats following PM2.5 and water-soluble components exposure.


Assuntos
Pulmão/imunologia , Material Particulado/imunologia , Fibrose Pulmonar/imunologia , Transdução de Sinais/imunologia , Proteína Smad3/imunologia , Fator de Crescimento Transformador beta1/imunologia , Animais , Asma/imunologia , Modelos Animais de Doenças , Masculino , Ovalbumina/imunologia , Ratos , Ratos Sprague-Dawley , Água
16.
J Vis Exp ; (172)2021 06 08.
Artigo em Inglês | MEDLINE | ID: mdl-34180875

RESUMO

The mosquito Aedes aegypti (Linnaeus), a vector of many important human diseases including yellow fever, dengue fever and Zika fever, shows a strong preference for human hosts over other warm-blooded animals for blood meals. Olfactory cues play a critical role for mosquitoes as they explore their environment and seek a human host to obtain blood meals, thus transmitting human diseases. Odorant receptors (ORs) expressed in the olfactory sensory neurons are known to be responsible for the interaction of mosquito vectors with human odors. To gain deeper insights into Ae. aegypti's olfactory physiology and investigate their interactions with humans at the molecular level, we used an optimized protocol of Xenopus Oocytes heterologous expression to functionally analyze Ae. aegypti odorant receptors in response to human odors. Three example experiments are presented: 1) Cloning and synthesizing cRNAs of ORs and odorant receptor co-receptor (Orco) from four to six days old Ae. aegypti antennae; 2) Microinjection and expression of ORs and Orco in Xenopus oocytes; and 3) Whole-cell current recording from Xenopus oocytes expressing mosquito ORs/Orco with a two-electrode voltage-clamp. These optimized procedures provide a new way for researchers to investigate human odor reception in Aedes mosquitoes and reveal the underlying mechanisms governing their host-seeking activity at a molecular level.

17.
Int Immunopharmacol ; 96: 107788, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34162152

RESUMO

Water-soluble ions (WSI) and organic extract (OE) in traffic-related particulate matter with aerodynamic diameters ≤ 2.5 µm (TRPM2.5) are potential risk factors for asthma exacerbation. Although CD4+ T lymphocytes mediated immune response is involved in the pathogenesis of asthma, the effect of WSI-TRPM2.5 and OE-TRPM2.5 on the balance of Th17/Treg cells in asthma remains poorly understood. In this study, the ovalbumin (OVA)-sensitized rats were repeatedly exposure to TRPM2.5 (3 mg/kg·bw), WSI-TRPM2.5 (1.8 mg/kg·bw, 7.2 mg/kg·bw) and OE-TRPM2.5 (0.6 mg/kg·bw, 2.4 mg/kg·bw) every three days for five times. The inflammation response and hyperemia edema were observed in the lung and trachea tissues. DNA methylation levels of STAT3 and RORγt genes in rats with WSI-TRPM2.5 and OE-TRPM2.5 treatment were decreased. DNA methylation level in STAT5 gene tended to decrease, with no change observed on Foxp3 expression. WSI-TRPM2.5 and OE-TRPM2.5 enhanced the mRNA and protein expression of STAT3 and RORγt while inhibited the expression of STAT5 and Foxp3, which may contribute to the imbalance of Th17/Treg cells (P < 0.05). More importantly, recovered balance of Th17/Treg cell subsets, upregulated p-STAT5 and Foxp3 expression and reduced p-STAT3 and RORγt levels were observed after 5-Aza treatment. Our results demonstrate that the STAT3/RORγt-STAT5/Foxp3 signaling pathway is involved in asthma exacerbation induced by WSI-TRPM2.5 and OE-TRPM2.5 through disrupting the balance of Th17/Treg cells. The alteration of DNA methylation of STAT3, STAT5, and RORγt genes may be involved in asthma exacerbation as well.

18.
Artigo em Inglês | MEDLINE | ID: mdl-34069341

RESUMO

This study aimed to examine how organizational behavior is associated with work engagement (WE) and work-home conflicts (WHCs) of physicians. The data were from a national cross-sectional survey of 3255 Chinese physicians. We examined organizational fairness, leadership attention, and team interaction for organizational behavior. The results indicate that greater organizational fairness is associated with higher WE and lower WHCs. High task fairness was associated with greater pride, and more enjoyment in work, lower sense of guilt towards their family, and less complaints from family members. Physicians reporting higher levels of leaders' attention to their opinions reported experiencing more enjoyment of their work, and less effects on their care for family. A greater number of dinners with colleagues per month was associated with higher WE and lower WHCs, whilst a greater number of clinical case meetings per month was associated with higher WE and higher WHCs. The results suggest that the behavior of organizations could be an important intervention to improve the wellbeing of physicians.


Assuntos
Médicos , Engajamento no Trabalho , China , Estudos Transversais , Humanos , Liderança
19.
Theor Appl Genet ; 134(8): 2469-2479, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33987716

RESUMO

KEY MESSAGE: Powdery mildew resistance gene MlIW39, originated from wild emmer wheat accession IW39, was mapped to a 460.3 kb genomic interval on wheat chromosome arm 2BS. Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is destructive disease and a significant threat to wheat production globally. The most effective way to control this disease is genetic resistance. However, when resistance genes become widely deployed in agriculture, their effectiveness is compromised by virulent variants that were previously minor components of the pathogen population or that arise from mutation. This necessitates continual search for new sources of resistance in both wheat and its near relatives. In this study, we produced a common wheat line 8D49 (87-1/IW39//2*87-1), which has all-stage immunity to Bgt isolate E09 and many other Chinese Bgt isolates, by transferring powdery mildew resistance from Israeli wild emmer wheat (WEW) accession IW39 to the susceptible common wheat line 87-1. Genetic analysis indicated that the powdery mildew resistance in 8D49 was controlled by a single dominant gene, temporarily designated MlIW39. Genetic linkage analyses with molecular markers showed that MlIW39 was located in a 0.7 cm genetic region between markers QB-3-16 and 7Seq546 on the short arm of chromosome 2B. Fine mapping using three large F2 populations delimited MlIW39 to a physical interval of approximately 460.3 kb region in the WEW reference genome (Zavitan v1.0) that contained six annotated protein-coding genes, four of which had gene structures similar to known disease resistance genes. This provides a foundation for map-based cloning of MlIW39. Markers 7Seq622 and 7Seq727 co-segregating with MlIW39 can be utilized for marker-assisted selection in further genetic studies and wheat breeding.


Assuntos
Ascomicetos/fisiologia , Mapeamento Cromossômico/métodos , Cromossomos de Plantas/genética , Resistência à Doença/imunologia , Doenças das Plantas/imunologia , Proteínas de Plantas/metabolismo , Triticum/genética , Resistência à Doença/genética , Regulação da Expressão Gênica de Plantas , Doenças das Plantas/genética , Doenças das Plantas/microbiologia , Proteínas de Plantas/genética , Triticum/crescimento & desenvolvimento , Triticum/microbiologia
20.
Pharmacol Res ; 169: 105650, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-33964468

RESUMO

Stroke is one of the leading causes of death worldwide. Hemorrhagic transformation (HT) is a common serious complication of ischemic stroke (IS) and is related to poor prognosis. Hyperglycemia after stroke is associated with the occurrence of HT and seriously affects the clinical treatment of stroke. Our previous experiments demonstrated that the Xiao-Xu-Ming decoction effective components group (XXMD), which is a Chinese medicine formula reconstituted by active ingredients, has multiple pharmacological effects in the treatment of IS. However, the effects of XXMD on HT after IS remain unclear. Thus, we investigated the preventive effects of XXMD on hyperglycemia-induced HT and further explored the underlying mechanism. Acute hyperglycemia combined with the electrocoagulation cerebral ischemia model was used to establish the HT model. XXMD (37.5, 75, 150 mg/kg/d) was given by gavage for 5 days. Network pharmacology was used to predict potential targets and pathways of XXMD in HT occurrence, and further studies confirmed the related targets. The results showed that hyperglycemia aggravated neurological deficits and blood-brain barrier (BBB) disruption, leading to intracerebral hemorrhage. Pretreatment with XXMD improved neurological function and BBB integrity and inhibited HT occurrence. Network pharmacology revealed that AGE-RAGE-mediated neuroinflammation may be associated with hyperglycemia-induced HT. Further studies confirmed that hyperglycemia activated the AGE-RAGE signaling pathway, increased the expression of HMGB1, TLR4 and p-p65, and induced the release of inflammatory factors and neutrophil infiltration, leading to HT. XXMD could inhibit AGE-RAGE-mediated neuroinflammation. These findings indicated that pretreatment with XXMD alleviated hyperglycemia-induced HT, which may be associated with the inhibition of AGE-RAGE-mediated neuroinflammation. Therefore, XXMD may be a potential therapeutic drug for HT.

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