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1.
Chemistry ; 2022 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-35781896

RESUMO

Metal-organic frameworks (MOFs) constructed by the ligands and metal clusters, have been considered as a promising material for photocatalytic water splitting. In this work, a solvent-assisted ligand exchange (SALE) method has been applied through partial reverse substitution of the ligand in NH 2 -MIL-125 (Ti) by 1,4-dicarboxybenzene (BDC). This modification strategy can optimize the charge transfer dynamics together with the preserved light absorption, resulting in a 3.3 times enhancement of the hydrogen production rate than the pristine material under visible-light irradiation. This work broadens the pathway of the ligand modifications of MOFs to boost the photocatalytic performance.

2.
Phys Chem Chem Phys ; 24(30): 18419-18426, 2022 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-35880864

RESUMO

Materials with superconductivity and a nontrivial band structure near the Fermi level are promising candidates in realizing topological superconductivity. Using first-principles calculations, we systematically investigated the stability, mechanical properties, superconductivity, electronic structures, and topological states of hexagonal TaC and NbC. The results show that they are stable and have excellent mechanical properties. We predicted that these two carbides are strong electron-phonon coupling superconductors with superconducting transition temperatures of 14.8 and 17.1 K, respectively. Strong coupling is mainly dominated by in-plane Ta/Nb atomic vibrations and in-plane Ta/Nb-dxy/dx2-y2 electronic orbitals. The electronic structure calculations demonstrate that a nodal line and a triply degenerate point coexist when not including the spin-orbit coupling (SOC) effect. After including the SOC effect, the nodal line is gapped. The complicated surface states are also calculated and need further experiments to verify. The present results indicate that the hexagonal TaC and NbC are potential candidates as topological superconductors, and pave the way towards exploring the superconductivity and topological materials in condensed matter systems.

3.
Cell Biol Int ; 2022 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-35723244

RESUMO

This study was designed and conducted to clarify the impact of RNF128 expression on malignant biological behaviors of colorectal cancer (CRC) cells and the underlying mechanism. The expression of RNF128 in CRC tissues was analyzed using mRNA sequencing data of TCGA database and was validated by Western blot assay. The experimental studies on biological functions of RNF128 in vitro were conducted to assess its impact on the proliferation, apoptosis, and metastasis of CRC cells. Furthermore, tumor xenograft models in nude mice were established to investigate the relationship between RNF128 expression and tumor growth in vivo. The expression levels of both RNF128 mRNA and protein were significantly increased in CRC tissues (p < .001). The knockdown of RNF128 markedly suppressed the malignant phenotype of HCT116 and SW480 cells in vitro, including cell growth, antiapoptosis, migration, and invasion (p < .001). On the other hand, knockdown of RNF128 exerted a remarkable effect on the growth inhibition of tumor xenografts in vivo (p < .001). Further investigation revealed that RNF128 knockdown lead to a significant decrease in the expression of p-AKT and p-PI3K protein. More importantly, the proliferative, antiapoptotic, metastatic abilities of RNF128-knockdown cells were markedly increased by 740 Y-P treatment (p < .001). These findings further suggested that PI3K/AKT signaling pathway played a key role in RNF128-mediated aggressive phenotype of CRC cells. RNF128 functions as a tumor promoter in the pathogenesis of CRC via regulating PI3K/AKT pathway, and it could be a valuable target for CRC treatment.

4.
Medicine (Baltimore) ; 101(25): e29434, 2022 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-35758378

RESUMO

BACKGROUND: In recent years, clinical studies have found that there is a close relationship between osteoporosis and polycystic ovary syndrome. However, there are few literature on the pathogenesis of osteoporosis and polycystic ovary syndrome. In order to clarify their common pathogenic mechanism and provide potential targets for drugs to regulate them at the same time, bioinformatics methods are used to explore, so as to provide a new direction for the study of the relationship between diseases in the future. METHODS: To screen the targets of osteoporosis and polycystic ovary syndrome by Genecards, Online Mendelian Inheritance in Man databases and Therapeutic Target Database to take the intersection of the two mappings and upload the intersection targets to the STRING database to construct protein-protein interaction network; to screen the core targets by degree value and import them to Metascape database for Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis; and finally, to construct the visualization network of core targets and pathways by Cytoscape software. Ethical approval and informed consent of patients are not required because the data used in this study is publicly available and does not involve individual patient data or privacy. RESULTS: The core targets of polycystic ovary syndrome and osteoporosis were insulin gene, insulin-like growth factor 1, CTNNB1, serine/threonine kinase 1, signal transducer and activator of transcription 3, LEP, etc. The biological processes involved include the regulation of protein phosphorylation, cell proliferation and differentiation, hormone endocrine, reproductive system and skeletal system. The related pathways were concentrated in Foxo signaling pathway, HTLV-I infection, PI3K-AKT signaling pathway, MAPK signaling pathway and AGE-RAGE signaling pathway in diabetic complications. CONCLUSIONS: There is a close relationship between osteoporosis and polycystic ovary syndrome in terms of target and molecular mechanism. This study used bioinformatics to clarify their targets and mechanisms, providing potential targets for drugs to regulate both diseases simultaneously and providing new directions to explore the relationship between the diseases.


Assuntos
Medicamentos de Ervas Chinesas , Osteoporose , Síndrome do Ovário Policístico , Biologia Computacional , Medicamentos de Ervas Chinesas/uso terapêutico , Feminino , Humanos , Osteoporose/tratamento farmacológico , Osteoporose/genética , Fosfatidilinositol 3-Quinases/metabolismo , Síndrome do Ovário Policístico/complicações , Síndrome do Ovário Policístico/tratamento farmacológico , Síndrome do Ovário Policístico/genética
5.
Biosens Bioelectron ; 211: 114393, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-35609450

RESUMO

A fast and simple Cas13a-based assay approach for direct detecting Ebola RNA in unamplified samples is reported. The procedure (named Cas-Roller) is comprised of a 10-min Cas13a-mediated cleavage protocol, followed by a DNA roller running for 30 min. This involves Cas13a collateral cleaving a suitably designed substrate in the presence of Ebola virus RNA sequence, and the cleavage product is used for DNA roller to amplify and generate fluorescent signals. After optimization of the conditions, the assay is able to achieve a limit of detection as low as 291 aM (∼175 copies RNA/µL) along with excellent anti-interfering performance in human serum and blood detection, which is ∼310-fold improved compared with the direct CRISPR assay. The entire workflow can be completed in ∼40 min at 37 °C without any pre-amplification, transcription, or centrifugation steps, thus avoiding the generation of false-negative or positive results. In addition, the downstream roller reaction is independent of the target sequence, this method can be applied to detect any other RNA by merely redesigning the hybridization regions of the crRNA. Overall, this strategy gives a new idea for the construction of simple and accurate Cas13a-based assays for the direct detection of RNA.


Assuntos
Técnicas Biossensoriais , Doença pelo Vírus Ebola , Sistemas CRISPR-Cas/genética , DNA , Doença pelo Vírus Ebola/diagnóstico , Doença pelo Vírus Ebola/genética , Humanos , RNA
6.
Sci Total Environ ; 837: 155847, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-35550889

RESUMO

The Bohai Sea, adjacent to the Northwest Pacific, is a semi-enclosed shallow-water marginal sea that was considered on a critical path of eutrophication and environmental degradation. To better understand the Bohai Sea metabolism-induced summertime dissolved oxygen (DO) decline, five field surveys were conducted between July 2019 and July 2021 to investigate the seasonal/interannual and spatial variations in DO, dissolved inorganic carbon (DIC), and stable isotopic composition of DIC (δ13CDIC). Although the water-mixing scheme was subject to spatial variation, a uniform apparent ratio of δ13CDIC versus apparent oxygen utilization was estimated at -0.0122‰ per µmol O2 kg-1 in the Bohai Sea in summer. Based on a three-endmember water-mixing model and the mass balance of DIC and its stable isotopic composition, the assumed uniform δ13C values of oxygen-consuming organic matter in the Bohai Sea DO-deficient areas was estimated to be -19.47 ± 1.85‰ in 2020 and between -20.6‰ and - 18.1‰ in 2021. This isotopic composition is very similar to the δ13C value of organic matter from marine diatoms, but different to that of terrestrial organic matter sources surrounding the Bohai Sea. Our results indicate that nearly all the organic matter consumed by community respiration in the Bohai Sea is produced in situ by marine plankton. To mitigate the seasonal DO shortage in the Bohai Sea, reduction of allochthonous nutrients is crucial.


Assuntos
Carbono , Oxigênio , Carbono/análise , Isótopos de Carbono/análise , China , Dacarbazina , Monitoramento Ambiental , Oceanos e Mares , Água
7.
Nanomicro Lett ; 14(1): 121, 2022 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-35505158

RESUMO

Bismuth-based materials (e.g., metallic, oxides and subcarbonate) are emerged as promising electrocatalysts for converting CO2 to formate. However, Bio-based electrocatalysts possess high overpotentials, while bismuth oxides and subcarbonate encounter stability issues. This work is designated to exemplify that the operando synthesis can be an effective means to enhance the stability of electrocatalysts under operando CO2RR conditions. A synthetic approach is developed to electrochemically convert BiOCl into Cl-containing subcarbonate (Bi2O2(CO3)xCly) under operando CO2RR conditions. The systematic operando spectroscopic studies depict that BiOCl is converted to Bi2O2(CO3)xCly via a cathodic potential-promoted anion-exchange process. The operando synthesized Bi2O2(CO3)xCly can tolerate - 1.0 V versus RHE, while for the wet-chemistry synthesized pure Bi2O2CO3, the formation of metallic Bio occurs at - 0.6 V versus RHE. At - 0.8 V versus RHE, Bi2O2(CO3)xCly can readily attain a FEHCOO- of 97.9%, much higher than that of the pure Bi2O2CO3 (81.3%). DFT calculations indicate that differing from the pure Bi2O2CO3-catalyzed CO2RR, where formate is formed via a *OCHO intermediate step that requires a high energy input energy of 2.69 eV to proceed, the formation of HCOO- over Bi2O2(CO3)xCly has proceeded via a *COOH intermediate step that only requires low energy input of 2.56 eV.

8.
Materials (Basel) ; 15(9)2022 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-35591480

RESUMO

Using density functional theory and semiclassical Boltzmann transport equation, the lattice thermal conductivity and electronic transport performance of monolayer SnI2 were systematically investigated. The results show that its room temperature lattice thermal conductivities along the zigzag and armchair directions are as low as 0.33 and 0.19 W/mK, respectively. This is attributed to the strong anharmonicity, softened acoustic modes, and weak bonding interactions. Such values of the lattice thermal conductivity are lower than those of other famous two-dimensional thermoelectric materials such as MoO3, SnSe, and KAgSe. The two quasi-degenerate band valleys for the valence band maximum make it a p-type thermoelectric material. Due to its ultralow lattice thermal conductivities, coupled with an ultrahigh Seebeck coefficient, monolayer SnI2 possesses an ultrahigh figure of merits at 800 K, approaching 4.01 and 3.34 along the armchair and zigzag directions, respectively. The results indicate that monolayer SnI2 is a promising low-dimensional thermoelectric system, and would stimulate further theoretical and experimental investigations of metal halides as thermoelectric materials.

9.
Small ; 18(19): e2200303, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35388963

RESUMO

High-valence metal-doped multimetal (oxy)hydroxides outperform noble metal electrocatalysts for the oxygen evolution reaction (OER) owing to the modified energetics between 3d metals and high-valence dopants. However, the rational design of sufficient and subtle modulators is still challenging. With a multimetal layered double hydroxide (LDH) as the OER catalyst, this study introduces a series of operando high-valence dopants (Cr, Ru, Ce, and V), which can restrict the 3+ valence states in the LDH template to prevent phase separation and operando transfer to the >3+ valence states for sufficient electronic interaction during the OER process. Through density functional theory simulations, ultrathin Cr-doped NiFe (NiFeCr) LDH is synthesized with strong electronic interaction between Cr dopants and NiFe bimetallic sites, evidenced by X-ray absorption spectroscopy. The resulting NiFeCr-LDH catalyzes the OER with ultralow overpotentials of 189 and 284 mV, obtaining current densities of 10 and 1000 mA cm-2 , respectively. Further, a NiFeCr-LDH anode is coupled in the anion exchange membrane electrolyzers to promote alkaline water splitting and CO2 -to-CO electrolysis, which achieves low full cell voltages at high current densities.

10.
Angew Chem Int Ed Engl ; 61(28): e202202298, 2022 Jul 11.
Artigo em Inglês | MEDLINE | ID: mdl-35389544

RESUMO

Electrochemical CO2 -to-CO conversion provides a possible way to address problems associated with the greenhouse effect; however, developing low-cost electrocatalysts to mediate high-efficiency CO2 reduction remains a challenge on account of the limited understanding of the nature of the real active sites. Herein, we reveal the Znδ+ metalloid sites as the real active sites of stable nonstoichiometric ZnOx structure derived from Zn2 P2 O7 through operando X-ray absorption fine structure analysis in conjunction with evolutionary-algorithm-based global optimization. Furthermore, theoretical and experimental results demonstrated that Znδ+ metalloid active sites could facilitate the activation of CO2 and the hydrogenation of *CO2 , thus accelerating the CO2 -to-CO conversion. Our work establishes a critical fundamental understanding of the origin of the real active center in the zinc-based electrocatalysts for CO2 reduction reaction.

11.
Open Life Sci ; 17(1): 344-350, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35480487

RESUMO

Complex forms of diabetes are the ultimate common pathway involving multiple genetic variations and multiple environmental factors. Type 2 diabetes (T2DM) is classified as complex diabetes. Varying degrees of insulin deficiency and tissue insulin resistance are two key links to T2DM. The islet ß cell dysfunction plays a crucial role in the pathogenesis of T2DM. The decompensation of the islet ß cell to insulin resistance is a common mechanism leading to the pathogenesis of T2DM. Available data show that genetic factors mainly affect cell function. At present, a number of susceptibility genes related to T2DM have been reported at home and abroad. In this study, the diabetes-related genes in the case of early-onset diabetes with a significant family history were examined, and our results showed the presence of the intron mutations of catalase (CAT) gene and hepatocyte nuclear factor 1ß (HNF1ß) gene. The patient enrolled in this study was observed and analyzed, thus, increasing further understanding of the genes associated with diabetes and exploring the pathogenesis of diabetes from the molecular level. This is significant for guiding the prevention, treatment, and prognosis evaluation of diabetes.

12.
Phys Chem Chem Phys ; 24(12): 7303-7310, 2022 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-35262117

RESUMO

Combining density functional theory (DFT) and semi-classic Boltzmann transport theory, we report the thermoelectric (TE) performance of a family of two-dimensional (2D) group IB-selenides XSe (X = Cu, Ag, Au). The results show that these monolayers exhibit small and anisotropic phonon velocities (0.98-3.84 km s-1), large Grüneisen parameters (up to 100), and drastic phonon scattering between the optical and acoustic phonons. These intrinsic properties originate from strong phonon anharmonicity and suppress the heat transport capacity, resulting in low lattice thermal conductivities (12.54 and 1.22 W m-1 K-1) along the x- and y-directions for a CuSe monolayer. Among our studied monolayers, the 2D CuSe monolayer possesses the most remarkable TE performance with ultrahigh ZT (3.26) for n-type doping along the y-direction at 300 K. CuSe monolayer can achieve higher thermoelectric conversion efficiency at a lower synthetic preparation cost than the expensive AgSe and AuSe monolayers, and our work provides a theoretical basis for paving the way for further experimental studies.

13.
J Am Chem Soc ; 144(13): 6028-6039, 2022 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-35302356

RESUMO

Water-alkaline electrolysis holds a great promise for industry-scale hydrogen production but is hindered by the lack of enabling hydrogen evolution reaction electrocatalysts to operate at ampere-level current densities under low overpotentials. Here, we report the use of hydrogen spillover-bridged water dissociation/hydrogen formation processes occurring at the synergistically hybridized Ni3S2/Cr2S3 sites to incapacitate the inhibition effect of high-current-density-induced high hydrogen coverage at the water dissociation site and concurrently promote Volmer/Tafel processes. The mechanistic insights critically important to enable ampere-level current density operation are depicted from the experimental and theoretical studies. The Volmer process is drastically boosted by the strong H2O adsorption at Cr5c sites of Cr2S3, the efficient H2O* dissociation via a heterolytic cleavage process (Cr5c-H2O* + S3c(#) → Cr5c-OH* + S3c-H#) on the Cr5c/S3c sites in Cr2S3, and the rapid desorption of OH* from Cr5c sites of Cr2S3 via a new water-assisted desorption mechanism (Cr5c-OH* + H2O(aq) → Cr5c-H2O* + OH-(aq)), while the efficient Tafel process is achieved through hydrogen spillover to rapidly transfer H# from the synergistically located H-rich site (Cr2S3) to the H-deficient site (Ni3S2) with excellent hydrogen formation activity. As a result, the hybridized Ni3S2/Cr2S3 electrocatalyst can readily achieve a current density of 3.5 A cm-2 under an overpotential of 251 ± 3 mV in 1.0 M KOH electrolyte. The concept exemplified in this work provides a useful means to address the shortfalls of ampere-level current-density-tolerant Hydrogen evolution reaction (HER) electrocatalysts.

14.
Phys Chem Chem Phys ; 24(13): 7893-7900, 2022 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-35302567

RESUMO

Based on first-principles calculations, we predict five global stable molybdenum phosphorus compounds in the pressure range of 0-300 GPa. All of them display superconductivity with different transition temperatures. Meanwhile, we find that a metastable crystal hex-MoP2, crystallized in a noncentrosymmetric structure, is a double-Weyl semimetal and the Weyl point is in the H-K path. The long Fermi arcs and the topological surface states, which can be observed by angle-resolved photoemission spectroscopy, emerge at the (100) surface below the Fermi level. Furthermore, we find that the superconductivity in hex-MoP2 can be enhanced by carrier doping. Due to the breaking of inversion symmetry, the unconventional spin-triplet pairing coexists with spin-singlet pairing in channel . Based on our theoretical model, there are the superconducting band gaps in both pairings. Our work provides a new platform of hex-MoP2 for studying both topological double-Weyl semimetal and superconductivity.

15.
iScience ; 25(1): 103625, 2022 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-35106466

RESUMO

Thalamic reticular nucleus (TRN) is known to be crucial for dynamically modulating sensory processing. Recently, the functional role of TRN in itch and pain sensation processing has drawn much attention. We found that ventrobasal thalamus (VB) neurons exhibited scratching behavior-related and nociceptive behavior-related neuronal activity changes, and most of VB neurons responsive to pruritic stimulus were also activated by nociceptive stimulus. Inhibition of VB could relieve itch-induced scratching behaviors and pathological pain without affecting basal nociceptive thresholds, and activation of VB could facilitate scratching behaviors. Tracing and electrophysiology recording results showed that VB mainly received inhibitory inputs from ventral TRN. Furthermore, optogenetic activation of TRN-VB projections suppressed scratching behaviors, and ablation of TRN enhanced scratching behaviors. In addition, activation of TRN-VB projections relieved the pathological pain without affecting basal nociceptive thresholds. Thus, our study indicates that TRN modulates itch and pain signals processing via TRN-VB inhibitory projections.

16.
Elife ; 112022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-35167440

RESUMO

Long-lasting negative affections dampen enthusiasm for life, and dealing with negative affective states is essential for individual survival. The parabrachial nucleus (PBN) and thalamic paraventricular nucleus (PVT) are critical for modulating affective states in mice. However, the functional roles of PBN-PVT projections in modulating affective states remain elusive. Here, we show that PBN neurons send dense projection fibers to the PVT and form direct excitatory synapses with PVT neurons. Activation of the PBN-PVT pathway induces robust behaviors associated with negative affective states without affecting nociceptive behaviors. Inhibition of the PBN-PVT pathway reduces aversion-like and fear-like behaviors. Furthermore, the PVT neurons innervated by the PBN are activated by aversive stimulation, and activation of PBN-PVT projections enhances the neuronal activity of PVT neurons in response to the aversive stimulus. Consistently, activation of PVT neurons that received PBN-PVT projections induces anxiety-like behaviors. Thus, our study indicates that PBN-PVT projections modulate negative affective states in mice.


Assuntos
Núcleos Parabraquiais , Animais , Camundongos , Neurônios/fisiologia , Sinapses
17.
Phys Chem Chem Phys ; 24(7): 4613-4619, 2022 Feb 16.
Artigo em Inglês | MEDLINE | ID: mdl-35132981

RESUMO

The discovery of new semiconducting materials with low thermal conductivity is of vital importance in promoting thermal energy conversion and management. Herein, lattice dynamical and thermal transport mechanism of new energetically stable 2D Ga2O3(100) is presented using density functional theory. The results show that 2D Ga2O3(100) possesses an extremely low lattice thermal conductivity of ∼0.71 W mK-1 at 300 K. We find that 2D Ga2O3(100) possesses two intrinsic features that decrease the lattice thermal conductivity: (1) the existence of interspersed distorted tetrahedral and pentahedral coordination geometries, which improves the phonon anharmonicity of the system; (2) compared to bulk ß-Ga2O3, the reduced dimensionality suppresses heat transfer by introducing interfacial scattering in 2D Ga2O3(100). Additionally, the strong Ga-O covalent bond results in a low speed of sound, high phonon-phonon scattering rates, and thus low lattice thermal conductivity. Our finding is remarkable because ultralow thermal conductivity can be realized in a simple 2D oxide, which provides replaceable materials for further applications in the field of thermal management.

18.
Chem Asian J ; 17(5): e202101359, 2022 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-35051308

RESUMO

A Rh2 O3 /BiVO4 composite photoanode exhibits enhanced surface reaction kinetics and charge transfer efficiency, enabling a photocurrent density of ca. 3.5 mA/cm2 at 1.23 V (vs. RHE), which is about 3.89 times higher than that of the pristine BiVO4 , with a lower onset potential of 0.29 V (vs. RHE).

19.
Angew Chem Int Ed Engl ; 61(2): e202111700, 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-34687123

RESUMO

Copper-based materials are efficient electrocatalysts for the conversion of CO2 to C2+ products, and most these materials are reconstructed in situ to regenerate active species. It is a challenge to precisely design precatalysts to obtain active sites for the CO2 reduction reaction (CO2 RR). Herein, we develop a strategy based on local sulfur doping of a Cu-based metal-organic framework precatalyst, in which the stable Cu-S motif is dispersed in the framework of HKUST-1 (S-HKUST-1). The precatalyst exhibits a high ethylene selectivity in an H-type cell with a maximum faradaic efficiency (FE) of 60.0 %, and delivers a current density of 400 mA cm-2 with an ethylene FE up to 57.2 % in a flow cell. Operando X-ray absorption results demonstrate that Cuδ+ species stabilized by the Cu-S motif exist in S-HKUST-1 during CO2 RR. Density functional theory calculations indicate the partially oxidized Cuδ+ at the Cu/Cux Sy interface is favorable for coupling of the *CO intermediate due to the modest distance between coupling sites and optimized adsorption energy.

20.
Sci Total Environ ; 813: 151786, 2022 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-34942265

RESUMO

In animals, the gut microbiome is vital to growth, and changes in the composition of these microbial communities may affect growth and adaptability to the environment. Temperature is another important factor that influences the healthy growth of animals. To date, the mechanism by which juvenile European seabass (Dicentrarchus labrax) and their symbiotic flora adapt to changes in environmental temperature is not well understood. Therefore, we evaluated the effect of temperature on the gut microbiota and metabolism of European seabass juveniles. We used 16S rRNA gene amplicon sequencing and non-targeted liquid chromatography with tandem mass spectrometry (LC-MS/MS)-based metabolomics to study the gut microbes of European seabass after 60 days of rearing of water temperature at 10 °C (T1), 15 °C (T2) and 20 °C (T3). At the phylum level, the abundance of the gut microbiota did not differ significantly among the three groups after 60 days of cultivation. At the genus level, however, the abundance of Faecalibacterium, Filifactor, Butyricicoccus, and Erysipelotrichaceae UCG-006 in the intestines differed significantly among the temperature groups. Compared with T2, the relative abundance of Filifactor in T1 was significantly increased, while Faecalibacterium was significantly decreased, while the relative abundance of Butyricicoccus and Erysipelotrichaceae UCG-006 in T3 was significantly increased. The LC-MS/MS analysis revealed 107 metabolites in the 10 °C group and 68 metabolites in the 20 °C group that differed significantly from those in the intestines of fish in the 15 °C control group. These metabolites are closely related to several metabolic pathways, including amino acid metabolism, glucose and lipid metabolism, and the tricarboxylic acid cycle. Correlation analysis of the Intestine microbiota, metabolic pathways, and metabolites identified metabolic pathways and metabolites that were strongly related to the observed significant differences in the microbiome among the temperature groups. These results show that temperature can induce significant changes in the gut microbiota and metabolism of European seabass juveniles, and that significant changes in metabolites may be mediated through the interaction of the microbiome and metabolic pathways.


Assuntos
Bass , Microbioma Gastrointestinal , Animais , Cromatografia Líquida , RNA Ribossômico 16S/genética , Espectrometria de Massas em Tandem , Temperatura
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