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1.
Biomed J ; 2020 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-32418767

RESUMO

BACKGROUND: MicroRNAs (miRNAs) play an important regulatory role in carcinogenesis and cancer progression. MiR-95-3p has been reported to be an oncogene in hepatocellular carcinoma. However, the role of miR-95-3p in colorectal carcinoma (CRC) remains unclear. METHODS: miR-95-3p was validated in an independent validation sample cohort of 215 CRC tissues. Functional assays, Cell proliferation (MTT) assay colony formation, wound healing, transwell and animal xenograft assays were used to determine the oppressor role of miR-95-3p in human CRC progression. Furthermore, Bioinformatics analysis, western blotting and dual-luciferase reporter assay were used to determine the mechanism by which miR-95-3p suppresses progression of CRC cells. RESULTS: In this study, we found that miR-95-3p was downregulated in CRC tissues. The low level of miR-95-3p in CRC tumors was correlated with aggressive clinicopathological characteristics, and it predicted poor prognosis in CRC patients. The overexpression of miR-95-3p significantly inhibited CRC cell proliferation, colony formation and metastasis in vitro and in vivo. Bioinformatic analysis further identified hepatoma-derived growth factor (HDGF) as a novel target of miR-95-3p in CRC cells. These findings suggest that miR-95-3p regulates CRC cell survival, partially through the downregulation of HDGF. CONCLUSIONS: Therefore, the miR-95-3p/HDGF axis might serve as a novel therapeutic target in patients with CRC.

2.
BMC Plant Biol ; 20(1): 5, 2020 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-31900117

RESUMO

BACKGROUND: In strawberry cultivation, continuous cropping (CC) obstacles seriously threaten production. A patented soil amendment (SA) can effectively relieve the CC obstacles to strawberry cultivation, but knowledge of the recovery mechanisms underlying this phenomenon is limited. RESULTS: In this study, transcriptomic profiling of strawberry roots in soil with and without the SA was conducted using RNA-Seq technology to reveal gene expression changes in response to SA treatment. In total, 188 differentially expressed genes (DEGs), including 144 upregulated and 44 downregulated DEGs, were identified. SA treatment resulted in genotype-dependent responses, and the response pattern, including an overall increase in the expression of nutrient transport genes and a decrease in the expression of defense response genes, may be a possible mechanism underlying recovery strategies in strawberry roots after the application of the SA to CC soil. We also found that 9 Hsp genes involved in plant defense pathways were all downregulated in the SA-treated roots. CONCLUSIONS: This research indicated that strawberry plants reallocated defense resources to development when SA treatment alleviated the stress caused by a CC soil environment. The present study provides an opportunity to reveal the fundamental mechanisms of the tradeoff between growth and defense in strawberry.


Assuntos
Fragaria/genética , Raízes de Plantas/genética , Transcriptoma/genética , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Proteínas de Choque Térmico/genética , Proteínas de Choque Térmico/metabolismo , Imunidade Vegetal/genética , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/metabolismo , Solo/química
3.
Surg Oncol ; 31: 67-74, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31541909

RESUMO

The molecular mechanisms governing the metastasis of colorectal cancer (CRC) are incompletely understood. In the present study, we found NOVA1 to be expressed at higher levels in CRC cell lines and tissue samples, and this upregulation was positively correlated with TNM stage (p = 0.034), poor differentiation (p = 0.001), and lymph node metastasis (p = 0.008). Both overall survival (OS) and relapse-free survival (RFS) were both significantly decreased in patients with high NOVA1 expression relative to those with low expression. Through a multivariate analysis, we determined that NOVA1 independently predicted poor outcomes in those with CRC. In further functional studies, we found that NOVA1 expression controlled the proliferation and invasive characteristics of CRC cells via a mechanism wherein NOVA1 bound and stabilized the IL6 mRNA, enhancing IL-6/JAK2/STAT3 signaling to in turn upregulate matrix metalloproteinases (MMPs) 2, 7, and 9. NOVA1 therefore plays key functional roles in regulating CRC progression, and our results further indicate that it serve as a valuable prognostic biomarker and potentially a target for therapeutic treatment in individuals with CRC.

4.
Sci Rep ; 9(1): 8423, 2019 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-31182725

RESUMO

WRKY transcription factors play important roles in many plant developmental processes and adaptation to the environment. However, little knowledge is available about the WRKY gene family in cultivated strawberry (Fragaria × ananassa Duch.), an important soft fruit worldwide. In this study, a total of 47 WRKY gene members were identified and renamed on the basis of their order on the chromosomes. According to their evolutionary events and conserved structure, the 47 FaWRKYs were divided into three major groups with several subgroups. A cis-element analysis showed that all FaWRKYs possessed at least one stress response-related cis-element. Comprehensive analysis, including phylogenetic analysis and expression profiling, based on real-time qPCR analysis in root, stem, leaf and fruit was performed on group III FaWRKY genes. The phylogenetic tree of the WRKY III genes in cultivated strawberry, wild Strawberry, Arabidopsis, tomato, and rice was divided into five clades. Additionally, the expression profiles of the FaWRKY genes in response to continuous cropping were further investigated based on RNA-seq data. FaWRKY25, FaWRKY32, and FaWRKY45, which are group III FaWRKY genes, were upregulated after continuous cropping. The level of reactive oxygen species (ROS) and the expression levels of PR1 and peroxidase were higher in continuous cropping (CC) than in non-continuous cropping (NCC). The results indicated that group III FaWRKYs might play an important role in continuous cropping. These results provide a foundation for genetic improvements for continuous cropping tolerance in cultivated strawberry.

5.
Microbiologyopen ; 8(9): e00823, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-30916451

RESUMO

The nematodes of genus Oscheius are insect parasites with a potential role as biological control agents. The composition of gut microbiota and its potential assistant role in the complex pathogenic mechanism of nematodes have been poorly illustrated. In this study, the intestinal bacteria associated with dauer juveniles of the nematode Oscheius chongmingensis Tumian were classified by 16S rDNA high-throughput sequencing. The raw reads were assigned to 845 operational taxonomic units (OTUs) after quality filtering. The results showed that the genus Ochrobactrum, with a proportion of 59.82%, was the most abundant genus, followed by 7.13% Bacillus, 4.7% Albidiferax, 4.26% Acinetobacter, and 3.09% Rhodococcus. The two dominant bacteria, Ochrobactrum and Bacillus, were further isolated by culturing on NBTA and LB medium respectively, and then identified as Ochrobactrum tritici and Bacillus cereus by morphological and 16S rDNA sequence analysis. Furthermore, the entomopathogenicity of these two bacterial species was studied. The results showed that O. tritici caused 93.33% mortality within 144 hr in the 4th -instar larvae of Galleria mellonella treated with 2 × 109  CFU/ml, whereas B. cereus showed 100% mortality at a concentration of 3.3 × 107  CFU/ml within 48 hr. These findings, especially the presence of O. tritici, which had not been found in other nematode species in the genus Oscheius, indicate that the associated nematode O. chongmingensis may have particular utility as a biocontrol agent.

6.
J Econ Entomol ; 112(3): 1299-1305, 2019 05 22.
Artigo em Inglês | MEDLINE | ID: mdl-30649362

RESUMO

Similar to other pear psylla species in Europe and America, Cacopsylla chinensis (Yang and Li) is one of the most important pests that causes yield loss in commercial pear orchards in China. To investigate effective essential oils as alternatives to conventional pesticides against C. chinensis, 26 essential oils derived from commonly used Chinese spices and medicinal herbs were screened for insecticidal activity. Among these, the essential oil from Perilla frutescens (L.) Britton leaves was the top performer; it exhibited strong and acute toxicity against pear psylla, with an LD50 value of 0.63 µg per adult. Then, we tested the constituents of the essential oil and its toxicity in the field. Field trials showed a 72% corrected reduction in the first-second-instar population 7 d after spraying P. frutescens leaf oil solution at a concentration of 1 mg/ml and a 47% corrected reduction at days 3 and 14. This report is the first to document the application of essential oil from P. frutescens leaves to control C. chinensis under field conditions. Our results suggest that P. frutescens oil can be considered a novel potential pesticide for C. chinensis control in pear orchards.


Assuntos
Hemípteros , Lamiaceae , Lamiales , Óleos Voláteis , Perilla frutescens , Animais , China , Europa (Continente)
7.
J Econ Entomol ; 111(6): 2908-2913, 2018 12 14.
Artigo em Inglês | MEDLINE | ID: mdl-30124914

RESUMO

Pear psyllids are among the most damaging pests in pear orchards, but little knowledge exists of psyllid species in cultivated pear orchards in China. In this study, DNA sequence analyses of the 16S rDNA and cytochrome oxidase I (COI) DNA regions were performed to identify pear psyllids from 28 regions of 20 provinces in China and to classify their genetic relationships to understand the origin of the species. The results showed that Cacopsylla chinensis Yang et Li (Hemiptera: Psyllidae) was found in most pear orchards in China, but Cacopsylla qianli (Hemiptera: Psyllidae) was found in only the cities of Guiyang (Guizhou province) and Xiangyang (Hubei province). The results for the 16S rDNA and COI regions were similar. Based on the nucleotide sequences and phylogenetic analyses of 16S rDNA and COI, C. chinensis could be divided into three groups: lineages I, II, and III. Based on 16S rDNA and COI, lineage II showed approximately 4% and 3% difference from lineage I, and lineage III showed approximately 12% and 9% difference from lineage I, respectively. C. chinensis lineage I was found in most provinces of China, while C. chinensis lineage II samples were mainly found in the Bohai rim region of China, and lineage III samples were found in Northeast China. The results of this study will provide information to pear producers regarding effective control measures to prevent further damage from pear psyllids.


Assuntos
Hemípteros/genética , Filogenia , Animais , China , Complexo IV da Cadeia de Transporte de Elétrons/genética , Hemípteros/classificação , Pyrus , RNA Ribossômico 16S/genética
8.
Molecules ; 23(5)2018 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-29772795

RESUMO

The essential oil of Stachys riederi var. japonica (Family: Lamiaceae) was extracted by hydrodistillation and determined by GC and GC-MS. A total of 40 components were identified, representing 96.01% of the total oil composition. The major compounds in the essential oil were acetanisole (15.43%), anisole (9.43%), 1,8-cineole (8.07%), geraniol (7.89%), eugenol (4.54%), caryophyllene oxide (4.47%), caryophyllene (4.21%) and linalool (4.07%). Five active constituents (acetanisole, anisole, 1,8-cineole, eugenol and geraniol) were identified by bioactivity-directed fractionation. The essential oil possessed fumigant toxicity against maize weevils (Sitophilus zeamais) and booklice (Liposcelis bostrychophila), with LC50 values of 15.0 mg/L and 0.7 mg/L, respectively. Eugenol and anisole exhibited stronger fumigant toxicity than the oil against booklice. 1,8-Cineole showed stronger toxicity, and anisole as well as eugenol exhibited the same level of fumigant toxicity as the essential oil against maize weevils. The essential oil also exhibited contact toxicity against S. zeamais adults and L. bostrychophila, with LC50 values of 21.8 µg/adult and 287.0 µg/cm², respectively. The results indicated that the essential oil of S. riederi var. japonica and its isolates show potential as fumigants, and for their contact toxicity against grain storage insects.


Assuntos
Inseticidas/química , Inseticidas/farmacologia , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Componentes Aéreos da Planta/química , Stachys/química , Cromatografia Gasosa-Espectrometria de Massas , Estrutura Molecular , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Espectroscopia de Prótons por Ressonância Magnética
9.
Molecules ; 22(2)2017 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-28134799

RESUMO

The screening of Chinese medicinal herbs for insecticidal principles showed that the essential oil of Echinops grijsii Hance roots possessed significant larvicidal activity against mosquitoes. The essential oil was extracted via hydrodistillation and its constituents were determined by gas chromatography-mass spectrometry (GC-MS) analysis. GC-MS analyses revealed the presence of 31 components, with 5-(3-buten-1-yn-1-yl)-2,2'-bithiophene (5-BBT, 27.63%), αterthienyl (α-T, 14.95%),1,8-cineole (5.56%) and cis-ß-ocimene (5.01%) being the four major constituents. Based bioactivity-directed chromatographic separation of the essential oil led to the isolation of 5-BBT, 5-(4-isovaleroyloxybut-1-ynyl)-2,2'-bithiophene (5-IBT) and αT as active compounds. The essential oil of E. grijsii exhibited larvicidal activity against the fourth instar larvae of Aedes albopictus, Anopheles sinensis and Culex pipiens pallens with LC50 values of 2.65 µg/mL, 3.43 µg/mL and 1.47 µg/mL, respectively. The isolated thiophenes, 5-BBT and 5-IBT, possessed strong larvicidal activity against the fourth instar larvae of Ae. albopictus(LC50 = 0.34 µg/mL and 0.45 µg/mL, respectively) and An. sinensis(LC50 = 1.36 µg/mL and 5.36 µg/mL, respectively). The two isolated thiophenes also had LC50 values against the fourth instar larvae of C. pipiens pallens of 0.12 µg/mL and 0.33 µg/mL, respectively. The findings indicated that the essential oil of E. grijsii roots and the isolated thiophenes have an excellent potential for use in the control of Ae.albopictus, An. sinensis and C. pipiens pallens larvae and could be used in the search for new, safer and more effective natural compounds as larvicides.


Assuntos
Culicidae/efeitos dos fármacos , Echinops (Planta)/química , Inseticidas/química , Inseticidas/farmacologia , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Raízes de Plantas/química , Animais , Cromatografia Gasosa-Espectrometria de Massas , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Parasitária , Compostos Fitoquímicos/química
10.
Medicine (Baltimore) ; 96(1): e5845, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28072746

RESUMO

BACKGROUND: Competing endogenous RNA (ceRNA) regulation is a novel hypothesized mechanism that states RNA molecules share common target microRNAs (miRNAs) and may competitively combine into the same miRNA pool. METHODS: Zinc finger protein 148 (ZNF148) and TOP2A expression were analyzed in 742 colorectal cancer (CRC) tissues using immunohistochemistry (IHC). ZNF148 mRNA, TOP2A mRNA, miR101, miR144, miR335, and miR365 expression were estimated in 53 fresh frozen CRC tissues by reverse transcription polymerase chain reaction. Mechanisms underpinning ceRNA were examined using bioinformatics, correlation analysis, RNA interference, gene over-expression, and luciferase assays. RESULTS: Protein levels of ZNF148 and TOP2A detected by IHC positively correlated (Spearman correlation coefficient [rs] = 0.431, P < 0.001); mRNA levels of ZNF148 and TOP2A also positively correlated (r = 0.591, P < 0.001). Bioinformatics analysis demonstrated that ZNF148 and TOP2A mRNA had 13 common target miRNAs, including miR101, miR144, miR335, and miR365. Correlation analysis demonstrated that levels of ZNF148 mRNA were negatively associated with levels of miR144, miR335, and miR365. Knockdown and overexpression tests showed that ZNF148 mRNA and TOP2A mRNA regulated each other in HCT116 cells, respectively, but not in Dicer-deficient HCT116 cells. Luciferase assays demonstrated that ZNF148 and TOP2A regulated each other through 3'UTR. Overexpression of ZNF148 mRNA and TOP2A mRNA caused significant downregulation of miR101, miR144, miR335, and miR365 in the HCT116 cells. We also found that knockdown of ZNF148 and TOP2A significantly promoted cell growth, and overexpression of ZNF148 and TOP2A inhibited cell proliferation, which was abrogated in Dicer-deficient HCT116 cells. CONCLUSION: ZNF148 and TOP2A regulate each other through ceRNA regulatory mechanism in CRC, which has biological effects on cell proliferation.


Assuntos
Antígenos de Neoplasias , Proliferação de Células/genética , Neoplasias Colorretais , DNA Topoisomerases Tipo II , Proteínas de Ligação a DNA , Fatores de Transcrição , Antígenos de Neoplasias/genética , Neoplasias Colorretais/genética , Neoplasias Colorretais/metabolismo , DNA Topoisomerases Tipo II/genética , Proteínas de Ligação a DNA/genética , Perfilação da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Humanos , Imuno-Histoquímica , MicroRNAs/análise , Proteínas de Ligação a Poli-ADP-Ribose , Fatores de Transcrição/genética , Dedos de Zinco
11.
Molecules ; 21(12)2016 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-27918451

RESUMO

A new pyrrolidine alkaloid named (Z)-3-(4-hydroxybenzylidene)-4-(4-hydroxyphenyl)-1-methylpyrrolidin-2-one was isolated from the ethanol extract of the root barks of Orixa japonica. The structure of the new alkaloid was elucidated on the basis of NMR and MS analysis. The compound exhibited larvicidal activity against the fourth instar larvae of Aedes aegypti (LC50 = 232.09 µg/mL), Anopheles sinensis (LC50 = 49.91 µg/mL), and Culex pipiens pallens (LC50 = 161.10 µg/mL). The new alkaloid also possessed nematicidal activity against Bursaphelenchus xylophilus (LC50 = 391.50 µg/mL) and Meloidogynein congnita (LC50 = 134.51 µg/mL). The results indicate that the crude ethanol extract of O. japonica root barks and its isolated pyrrolidine alkaloid have potential for development into natural larvicides and nematicides.


Assuntos
Alcaloides/farmacologia , Antinematódeos/farmacologia , Inseticidas/farmacologia , Casca de Planta/química , Pirrolidinas/farmacologia , Pirrolidinonas/farmacologia , Rutaceae/química , Aedes/efeitos dos fármacos , Animais , Anopheles/efeitos dos fármacos , Culex/efeitos dos fármacos , Larva/efeitos dos fármacos , Nematoides/efeitos dos fármacos , Extratos Vegetais/farmacologia , Raízes de Plantas/química , Pirrolidinonas/isolamento & purificação
12.
Molecules ; 21(10)2016 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-27669203

RESUMO

During a screening program for new agrochemicals from Chinese medicinal herbs, the ethanol extract of Notopterygium incisum rhizomes was found to possess strong nematicidal activity against the two species of nematodes, Bursaphelenchus xylophilus and Meloidogyne incognita. Based on bioactivity-guided fractionation, the four constituents were isolated from the ethanol extract and identified as columbianetin, falcarindiol, falcarinol, and isoimperatorin. Among the four isolated constituents, two acetylenic compounds, falcarindiol and falcarinol (2.20-12.60 µg/mL and 1.06-4.96 µg/mL, respectively) exhibited stronger nematicidal activity than two furanocoumarins, columbianetin, and isoimperatorin (21.83-103.44 µg/mL and 17.21-30.91 µg/mL, respectively) against the two species of nematodes, B. xylophilus and M. incognita. The four isolated constituents also displayed phototoxic activity against the nematodes. The results indicate that the ethanol extract of N. incisum and its four isolated constituents have potential for development into natural nematicides for control of plant-parasitic nematodes.


Assuntos
Apiaceae/química , Extratos Vegetais/farmacologia , Tylenchida/efeitos dos fármacos , Tylenchoidea/efeitos dos fármacos , Animais , Di-Inos/química , Medicamentos de Ervas Chinesas/farmacologia , Álcoois Graxos/química , Furocumarinas/química , Extratos Vegetais/química , Rizoma/química , Tylenchida/patogenicidade , Tylenchoidea/patogenicidade
13.
Medicine (Baltimore) ; 95(9): e2988, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26945418

RESUMO

Although the absolute number of positive lymph nodes (LNs) has been established as 1 of the most important prognostic factors in rectal cancers, many researchers have proposed that the lymph node ratio (LNR) may have better predicted outcomes. We conducted a retrospective study to compare the predictive ability of LNR and ypN category in rectal cancer. A total of 264 locally advanced rectal cancer (LARC) patients who underwent preoperative chemoradiotherapy (CRT) followed by total mesorectal excision (TME) between 2005 and 2012 were reviewed. All patients were categorized into 3 groups or patients with metastatic LNs were categorized into 2 groups according to the LNR. The prognostic effect on overall survival (OS) and disease-free survival (DFS) was evaluated. With a median follow-up of 45 months, the OS and DFS were 68.4% and 59.3% for the entire cohort, respectively. The respective 5-year OS and DFS rates for the 3 groups (LNR = 0, 0 < LNR ≤ 0.20, and 0.20 < LNR ≤ 1.0) were as follows: 83.2%, 72.6%, and 49.4% (P < 0.001) and 79.5%, 57.3%, and 33.5% (P < 0.001), respectively. Multivariate analysis revealed that LNR and differentiation, but not the number of positive LNs, had independent prognostic value for OS (hazard ratio [HR] = 2.328, 95% confidence interval [CI]: 1.850-4.526, P < 0.001) and DFS (HR = 3.004, 95% CI: 1.616-5.980, P < 0.001). As for patients with positive LNs, the respective 5-year OS and DFS rates for the 2 groups (0 < LNR ≤ 0.20, and 0.20 < LNR ≤ 1.0) were 72.6% and 49.4% (P < 0.001) and 57.3% and 33.5% (P < 0.001), respectively. Multivariate analysis revealed that only LNR was an independent factor for OS (HR = 3.214, 95% CI: 1.726-5.986, P < 0.001) and DFS (HR = 4.230, 95% CI: 1.825-6.458, P < 0.001). Subgroups analysis demonstrated that the ypN category had no impact on survival whereas increased LNR was a significantly prognostic indicator for worse survival in the LNs < 12 subgroup. LNR is an independent prognostic factor in LARC patients treated with preoperative CRT followed by TME. It may be a better independent staging method than the number of metastatic LNs when <12 LNs are harvested after preoperative CRT.


Assuntos
Adenocarcinoma/patologia , Antineoplásicos/uso terapêutico , Excisão de Linfonodo , Linfonodos/patologia , Neoplasias Retais/patologia , Reto/cirurgia , Adenocarcinoma/tratamento farmacológico , Adenocarcinoma/mortalidade , Adenocarcinoma/cirurgia , Adulto , Idoso , Idoso de 80 Anos ou mais , Quimiorradioterapia Adjuvante , Feminino , Seguimentos , Humanos , Linfonodos/cirurgia , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Terapia Neoadjuvante , Prognóstico , Neoplasias Retais/tratamento farmacológico , Neoplasias Retais/mortalidade , Neoplasias Retais/cirurgia , Reto/patologia , Estudos Retrospectivos , Análise de Sobrevida
14.
J Econ Entomol ; 108(3): 957-61, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26470216

RESUMO

Cacopsylla chinensis (Yang and Li) (Hemiptera: Psyllidae) is an important pest of pear in China. As an alternative to conventional chemical pesticides, botanicals including essential oils and their constituents could provide an eco-friendly and nonhazardous control method. In this study, the essential oil of clove buds (Syzygium aromaticum) was obtained by hydrodistillation. Five constituents, accounting for 99.89% of the oil, were identified by gas chromatography-mass spectrometry, and the major constituents were eugenol (88.61%) and eugenol acetate (8.89%), followed by ß-caryophyllene (1.89%). In a laboratory bioassay, clove essential oil, commercial eugenol (99.00%) and ß-caryophyllene (98.00%) exhibited strong contact toxicity against the summerform adults of C. chinensis with LD50 values of 0.730, 0.673, and 0.708 µg/adult, and against the nymphs with LD50 values of 1.795, 1.668, and 1.770 µg/nymph, respectively. In contrast, commercial eugenol acetate (98%) had LD50 values of 9.266 µg/adult and 9.942 µg/nymph. In a field trial, clove essential oil caused significant population reductions of 73.01% (4.80 mg/ml), 66.18% (2.40 mg/ml) and 46.56% (1.20 mg/ml), respectively. Our results demonstrated that clove essential oil and its constituents have potential as a source of natural insecticides.


Assuntos
Óleo de Cravo , Hemípteros , Inseticidas , Animais , Hemípteros/crescimento & desenvolvimento , Dose Letal Mediana , Ninfa/crescimento & desenvolvimento
15.
J Med Entomol ; 52(1): 86-92, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26336284

RESUMO

The aim of this research was to evaluate contact toxicity and repellency of the essential oil of Pogostemon cablin (Blanco) Bentham leaves against German cockroaches (Blattella germanica) (L.) and to isolate any active constituents. Essential oil of P. cablin leaves was obtained by hydrodistillation and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Twenty-three components were identified in the essential oil, and the main constituents were patchoulol (41.31%), pogostone (18.06%), α-bulnesene (6.56%), caryophyllene (5.96%), and seychellene (4.32%). Bioactivity-directed chromatographic separation of the essential oil led to the isolation of pogostone, patchoulol, and caryophyllene as active compounds. The essential oil of P. cablin leaves exhibited acute toxicity against male B. germanica adults with an LC50 value of 23.45 µg per adult. The constituent compound, pogostone (LC50 = 8.51 µg per adult) showed stronger acute toxicity than patchoulol (LC50 = 207.62 µg per adult) and caryophyllene (LC50 = 339.90 µg per adult) against the male German cockroaches. The essential oil of P. cablin leaves and the three isolated constituents exhibited strong repellent activity against German cockroaches at a concentration of 5 ppm. The results indicated that the essential oil of P. cablin leaves and its major constituents have good potential as a source for natural insecticides and repellents.


Assuntos
Blattellidae/efeitos dos fármacos , Repelentes de Insetos/toxicidade , Inseticidas , Lamiaceae/química , Óleos Voláteis/toxicidade , Animais , Blattellidae/crescimento & desenvolvimento , Cromatografia Gasosa-Espectrometria de Massas , Repelentes de Insetos/farmacologia , Inseticidas/farmacologia , Inseticidas/toxicidade , Masculino , Ninfa/efeitos dos fármacos , Ninfa/crescimento & desenvolvimento , Óleos Voláteis/farmacologia , Folhas de Planta/química
16.
J Food Prot ; 78(10): 1870-4, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26408136

RESUMO

Water-distilled essential oil from Clinopodium chinense (Labiatae) aerial parts at the flowering stage was analyzed by gas chromatography-mass spectrometry. Thirty-five compounds, accounting for 99.18% of the total oil, were identified, and the main components of the essential oil of C. chinense were spathulenol (18.54%), piperitone (18.9%), caryophyllene (12.04%), and bornyl acetate (8.14%). Based on bioactivity-directed fractionation, bornyl acetate, caryophyllene, and piperitone were identified from the essential oil. The essential oil possessed fumigant toxicity against booklice (Liposcelis bostrychophila) with a 50% lethal concentration (LC50) value of 423.39 µg/liter, while the isolated constituents, bornyl acetate and piperitone, had LC50 values of 351.69 and 311.12 µg/liter against booklice, respectively. The essential oil also exhibited contact toxicity against L. bostrychophila with an LC50 value of 215.25 µg/cm(2). Bornyl acetate, caryophyllene, and piperitone exhibited acute toxicity against booklice with LC50 values of 321.42, 275.00, and 139.74 µg/cm(2), respectively. The results indicated that the essential oil and its isolated constituents have potential for development into natural insecticides or fumigants for control of insects in stored grains.


Assuntos
Insetos/efeitos dos fármacos , Inseticidas/química , Lamiaceae/química , Óleos Voláteis/química , Componentes Aéreos da Planta/química , Animais , /farmacologia , Relação Dose-Resposta a Droga , Cromatografia Gasosa-Espectrometria de Massas , Inseticidas/toxicidade , Dose Letal Mediana , Monoterpenos/química , Monoterpenos/toxicidade , Óleos Voláteis/toxicidade , Sesquiterpenos/química , Sesquiterpenos/toxicidade , Testes de Toxicidade Aguda
17.
World J Gastroenterol ; 21(29): 8836-47, 2015 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-26269673

RESUMO

AIM: To investigate the expression and oncogenic role of nemo-like kinase (NLK) in colorectal cancer. METHODS: Expression of NLK protein was assessed by immunohistochemistry in tissue specimens from 56 cases of normal colorectal mucosa, 51 cases of colorectal adenoma, and 712 cases of colorectal cancer. In addition, NLK expression was knocked down using a lentivirus carrying NLK small hairpin RNA in colorectal cancer cells. Cell viability methylthiazoletetrazolium assays, colony formation assays, flow cytometry cell cycle assays, Transwell migration assays, and gene expression assays were performed to explore its role on proliferation and migration of colorectal cancer. RESULTS: Expression of NLK protein progressively increased in tissues from the normal mucosa through adenoma to various stages of colorectal cancer. Overexpression of NLK protein was associated with advanced tumor-lymph node-metastasis stages, poor differentiation, lymph node and distant metastases, and a higher recurrence rate of colorectal cancer (P < 0.05). Multivariate analyses showed that NLK expression was an independent prognostic factor to predict overall survival (hazard ratio 2.57, 95% confidence interval: 1.66-3.98; P < 0.001) and disease-free survival (hazard ratio 1.96, 95% confidence interval: 1.40-2.74: P < 0.001) of colorectal cancer patients. Furthermore, knockdown of NLK expression in colorectal cancer cell lines reduced cell viability, colony formation, and migration, and arrested tumor cells at the G0/G1 phase of the cell cycle. At the gene level, knockdown of NLK expression inhibited matrix metalloproteinase-2 expression in colorectal cancer cells. CONCLUSION: NLK overexpression is an independent prognostic factor in colorectal cancer and knockdown of NLK expression inhibits colorectal cancer progression and metastasis.


Assuntos
Pólipos Adenomatosos/enzimologia , Biomarcadores Tumorais/metabolismo , Carcinoma/enzimologia , Pólipos do Colo/enzimologia , Neoplasias Colorretais/enzimologia , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Pólipos Adenomatosos/genética , Pólipos Adenomatosos/mortalidade , Pólipos Adenomatosos/patologia , Pólipos Adenomatosos/cirurgia , Biomarcadores Tumorais/genética , Carcinoma/genética , Carcinoma/mortalidade , Carcinoma/secundário , Carcinoma/cirurgia , Pontos de Checagem do Ciclo Celular , Movimento Celular , Proliferação de Células , Sobrevivência Celular , Pólipos do Colo/genética , Pólipos do Colo/mortalidade , Pólipos do Colo/patologia , Pólipos do Colo/cirurgia , Neoplasias Colorretais/genética , Neoplasias Colorretais/mortalidade , Neoplasias Colorretais/patologia , Neoplasias Colorretais/cirurgia , Progressão da Doença , Intervalo Livre de Doença , Regulação Enzimológica da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Células HCT116 , Células HEK293 , Células HT29 , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/genética , Metástase Linfática , Análise Multivariada , Recidiva Local de Neoplasia , Estadiamento de Neoplasias , Proteínas Serina-Treonina Quinases/genética , Interferência de RNA , Fatores de Risco , Transfecção , Resultado do Tratamento , Regulação para Cima
18.
Z Naturforsch C J Biosci ; 70(1-2): 1-6, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25854838

RESUMO

The aim of this study was to evaluate the larvicidal activity of the essential oil of Youngia japonica aerial parts against the larvae of Aedes albopictus and to isolate any active compounds from the oil. Gas chromatography-mass spectrometry (GC-MS) analyses revealed the presence of 31 compounds, with menthol (23.53%), α-asarone (21.54%), 1,8-cineole (5.36%), and caryophyllene (4.45%) as the major constituents. Bioactivity-directed chromatographic separation of the oil led to the isolation of menthol and α-asarone as active compounds. The essential oil of Y. japonica exhibited larvicidal activity against the fourth instar larvae of A. albopictus with an LC50 value of 32.45 µg/mL. α-Asarone and menthol possessed larvicidal activity against the fourth instar larvae of A. albopictus with LC50 values of 24.56 µg/mL and 77.97 µg/mL, respectively. The results indicate that the essential oil of Y. japonica aerial parts and the two constituents can be potential sources of natural larvicides.


Assuntos
Aedes/embriologia , Asteraceae , Inseticidas , Controle de Mosquitos/métodos , Óleos Voláteis , Óleos Vegetais , Animais , Anisóis/isolamento & purificação , Asteraceae/química , Cromatografia Gasosa-Espectrometria de Massas , Inseticidas/isolamento & purificação , Larva , Mentol/isolamento & purificação , Óleos Voláteis/isolamento & purificação , Componentes Aéreos da Planta , Óleos Vegetais/isolamento & purificação
19.
Int J Clin Exp Pathol ; 8(10): 12802-10, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26722470

RESUMO

BACKGROUND: ITGB1 is a heterodimeric cell-surface receptor involved in cell functions such as proliferation, migration, invasion and survival. The aim of this study was to assess ITGB1 expression in colorectal cancer and correlate it with clinicopathological features, as well as to evaluate its potential prognostic significance. MATERIALS AND METHODS: In this study, we examined the expression of ITGB1 using tissue microarrays containing analyzed specimens by immunohistochemistry. ITGB1 expression was further correlated with clinicopathological and prognostic data. The prognostic significance was assessed using Kaplan-Meier survival estimates and log-rank tests. A multivariate study with the Cox's proportional hazard model was used to evaluate the prognostic aspects. RESULTS: ITGB1 expression was present in 88.5% of the analyzed specimens. Significant differences in ITGB1 expression were found between normal mucosa and carcinomas (P<0.001). High ITGB1 expression was associated with poor prognosis, and it independently correlated with shortened overall survival and disease-free survival in colorectal cancer patients (P<0.001). More so, ITGB1 expression, bowel wall invasion, lymph node metastasis and distant metastasis were independent prognostic factors for overall survival. Additionally, significant differences in ITGB1 expression were observed in adenomas and tumors from patients with familial adenomatous polyposis compared to normal colon mucosa (P<0.05) CONCLUSION: The results of this study indicate that ITGB1 overexpression in colorectal tumors is associated with poor prognosis, as well as aggressive clinicopathological features. Therefore, ITGB1 expression could be used as potential prognostic predictor in colorectal cancer patients.


Assuntos
Neoplasias Colorretais/metabolismo , Regulação Neoplásica da Expressão Gênica , Integrina beta1/metabolismo , Regulação para Cima , Biomarcadores Tumorais , Colo/metabolismo , Colo/patologia , Neoplasias Colorretais/mortalidade , Neoplasias Colorretais/patologia , Feminino , Humanos , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patologia , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Prospectivos , Taxa de Sobrevida , Análise Serial de Tecidos
20.
J Econ Entomol ; 107(4): 1706-12, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25195466

RESUMO

The aim of this research was to determine chemical composition and repellent and insecticidal activities of the essential oil of Kaempferia galanga L. rhizomes against the booklouse, Liposcelis bostrychophila Badonnel, and to isolate insecticidal or repellent constituents from the oil. The essential oil was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. Twenty-eight components of the oil were identified. The major compounds in the oil were ethyl-rho-methoxycinnamate (38.6%), ethyl cinnamate (23.2%), 1,8-cineole (11.5%), trans-cinnamaldehyde (5.3%), and borneol (5.2%). Based on bioactivity-guided fractionation, four active constituents were isolated from the oil and identified as 1,8-cineole, ethyl cinnamate, ethyl rho-methoxycinnamate, and trans-cinnamaldehyde. The essential oil exhibited contact toxicity against the booklouse with an LC50 value of 68.6 microg/cm2. Ethyl cinnamate (LC50 = 21.4 microg/cm2) exhibited stronger contact toxicity than ethyl rho-methoxycinnamate and trans-cinnamaldehyde (LC50 = 44.6 and 43.4 microg/cm2, respectively) while 1,8-cineole showed weak acute toxicity. The essential oil also possessed fumigant toxicity against the booklouse with a LC50 value of 1.5 mg/liter air. 1,8-Cineole and trans-cinnamaldehyde (LC50 = 1.1 and 1.3 mg/liter, respectively) possessed stronger fumigant toxicity against the booklouse than ethyl cinnamate and ethyl rho-methoxycinnamate (LC50 = 10.2 and 10.2 mg/liter air, respectively). trans-Cinnamaldehyde was strongly repellent to booklice, whereas ethyl cinnamate and ethyl rho-methoxycinnamate were weakly repellent and 1,8-cineole did not repel booklice. The results indicate that the essential oil and its constituent compounds have potential for development into natural insecticides or fumigants and repellents for control of insects in stored grains.


Assuntos
Repelentes de Insetos/análise , Insetos , Inseticidas/análise , Óleos Voláteis/química , Zingiberaceae/química , Animais , Rizoma/química
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