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1.
Eur Rev Med Pharmacol Sci ; 24(1): 409-417, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31957855

RESUMO

OBJECTIVE: To study the pro-apoptotic effect of Losartan on myocardial cells after acute myocardial infarction (AMI) in rats. MATERIALS AND METHODS: Before intervention, a total of 48 male Wistar rats were randomly divided into the Sham group (n=12), AMI group (n=12), 5 mg/kg Losartan group (n=12) and 1 mg/kg AG-490 group (n=12). The rats in the Sham group and AMI group received gavage with normal saline, those in the Losartan group received gavage with Losartan for 7 d and those in the AG-490 group were intravenously injected with AG-490 at 30 min before the operation. At 4 d after drug administration, the anterior descending coronary artery was ligated to establish the AMI model in the AMI group and Losartan group, while the same operation was performed, and the anterior descending coronary artery was only threaded in the Sham group. The rats were sacrificed at 24 h after operation. Then, the myocardial apoptosis was detected via terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay, and the protein expressions of Janus kinase 2 (JAK2), the signal transducer and activator of transcription 3 (STAT3), the B-cell lymphoma-2 (Bcl-2) and the Bcl-2 associated X protein (Bax) were detected via immunohistochemistry and Western blotting. Moreover, the myocardial cells of rats were incubated with angiotensin II (Ang II) at the same concentration at different time points and blocked with Losartan. Finally, the changes in protein expressions of p-JAK2, p-STAT3, Bax, and Bcl-2 were detected via Western blotting. RESULTS: Losartan treatment could increase the number of apoptotic myocardial cells after AMI in rats. In Losartan group, the protein expressions of JAK2, STAT3, and Bcl-2 declined, while the protein expression of Bax was increased, and the Bax/Bcl-2 ratio was also increased, which are consistent with the conditions under the treatment with the JAK-STAT pathway inhibitor AG-490. With the prolonged time of stimulation (5 min, 30 min, 2 h, and 24 h) in myocardial cells using 1.0×10-6 mol/L Ang II, the protein expressions of p-JAK2 and p-STAT3 were increased and reached the peak at 24 h. After the application of Losartan, the increased protein expressions of p-JAK2 and p-STAT3 returned to normal levels, and the protein expression of Bax was increased, while that of Bcl-2 was decreased. CONCLUSIONS: Losartan promotes myocardial apoptosis after AMI in the rats through inhibiting the Ang II-induced JAK/STAT pathway.

2.
Zhonghua Yan Ke Za Zhi ; 56(1): 41-46, 2020 Jan 11.
Artigo em Chinês | MEDLINE | ID: mdl-31937062

RESUMO

Objective: To evaluate the rotational stability of the Toric intraocular lens (TIOL) and influencing factors in cataract patients with different axial length. Methods: This retrospective cohort study consecutively enrolled patients who had phacoemulsification and AcrySof TIOL implantation in Peking University Third Hospital from May 2018 to January 2019. Based on axial length, patients were divided into two groups. Group A consisted of patients whose axial length was ≤ 24 mm. Patients whose axial length was >24 mm were included in group B. Data at three months postoperatively were used to evaluate the rotational stability of TIOL and its correlation with axial length, corneal white to white distance, lens thickness and TIOL spherical power. And t test, nonparametric test, chi-square test and Spearman test were used for statistical analysis. Results: Group A enrolled 39 patients (17 males and 22 females), with a median age of 74 years (range, 36-86 years). Group B enrolled 26 patients (11 males and 15 females), with a median age of 68 years (range, 36-86 years). For the efficacy of TIOL, in group A, the best corrected distance visual acuity (BCDVA, logarithm of the minimum angle of resolution) was 0.30 (0.10, 1.00) preoperatively and 0.10 (0.00, 0.60) postoperatively, and the astigmatism was 2.11 (0.95, 5.10) D preoperatively and 1.00 (0.00, 1.75) D postoperatively. In group B, the BCDVA was 0.36 (0.05, 1.00) preoperatively and 0.05 (0.00, 0.40) postoperatively, and the astigmatism was 2.00 (0.78, 3.76) D preoperatively and 0.75 (0.00, 2.25) D postoperatively. Between group A and group B, there were no significant differences in BCDVA (P=0.604) and astigmatism (P=0.789) preoperatively.In these two groups, postoperative BCDVA and astigmatism both significantly improved compared to preoperative parameters (both P<0.01). Between group A and group B, there were no significant differences in BCDVA (P=0.536) and astigmatism (P=0.076) postoperatively. In terms of rotational stability, the rotation in group A was 5.15°±3.62°, and that in group B was 6.50°±4.66°. There was no statistical difference between two groups (P=0.195). As for predictability, the percentage of eyes with rotation ≤5° was 59.0% (23 eyes) in group A and 50.0% (13 eyes) in group B. There was no statistical difference between the two groups (P=0.647). There was no significant correlation between the rotational stability of TIOL and axial length, corneal white to white distance, lens thickness or TIOL spherical power (P=0.836, 0.568, 0.170, 0.365). Conclusions: The rotational stability of TIOL at three months postoperatively in patients whose axial length >24 mm is of no difference with patients whose axial length ≤ 24 mm. It has no correlation with axial length, corneal white to white distance, lens thickness and TIOL spherical power. (Chin J Ophthalmol, 2020, 56: 41-46).


Assuntos
Astigmatismo/cirurgia , Comprimento Axial do Olho , Catarata/complicações , Implante de Lente Intraocular , Lentes Intraoculares , Facoemulsificação , Acuidade Visual/fisiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Astigmatismo/complicações , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Refração Ocular , Estudos Retrospectivos , Resultado do Tratamento
3.
Nat Commun ; 10(1): 5524, 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31797930

RESUMO

CRISPR-Cas systems have revolutionized genome editing across a broad range of biotechnological endeavors. Many CRISPR-Cas nucleases have been identified and engineered for improved capabilities. Given the modular structure of such enzymes, we hypothesized that engineering chimeric sequences would generate non-natural variants that span the kinetic parameter landscape, and thus provide for the rapid selection of nucleases fit for a particular editing system. Here, we design a chimeric Cas12a-type library with approximately 560 synthetic chimeras, and select several functional variants. We demonstrate that certain nuclease domains can be recombined across distantly related nuclease templates to produce variants that function in bacteria, yeast, and human cell lines. We further characterize selected chimeric nucleases and find that they have different protospacer adjacent motif (PAM) preferences and the M44 chimera has higher specificity relative to wild-type (WT) sequences. This demonstration opens up the possibility of generating nuclease sequences with implications across biotechnology.

4.
Fa Yi Xue Za Zhi ; 35(5): 607-612, 2019 Oct.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-31833298

RESUMO

Abstract: Gradation of Disability in Human Body Injuries (hereinafter referred to as Gradation) has been released and used since January 2017, and has become the most widely used standard in forensic science practice. This paper calculates and rates the visual system evaluation provisions of the current domestic disability evaluation criteria represented by the Gradation which used the methods of Guides to the Evaluation of Permanent Impairment (hereinafter referred to as GEPI) issued by American Medical Association (AMA). Through comparing, a good correlation between the provisions in Gradation and whole person impairment rating index in GEPI was shown. On the basis of this, suggestions are put forward to amend some provisions of Gradation, in order to provide reference for the revision and further improvement of domestic standards and provisions.


Assuntos
Avaliação da Deficiência , Pessoas com Deficiência , Guias como Assunto , American Medical Association , China , Medicina Legal/normas , Ciências Forenses , Humanos , Estados Unidos
5.
Beijing Da Xue Xue Bao Yi Xue Ban ; 51(6): 1169-1172, 2019 Dec 18.
Artigo em Chinês | MEDLINE | ID: mdl-31848524

RESUMO

This case report concerns a 34-year-old woman who had been diagnosed with ankylosing spondylitis (AS), fibromyalgia syndrome (FMS), osteoarthritis (OA), lumbar disc herniation and the like in different hospitals during the past 18 months. She had progressive osteoarthrosis, significant muscle weakness, gait abnormalities in weightbearing areas, however without typical inflammatory low back pain, while the treatment with non-steroidal anti-inflammatory drugs (NSAIDs) was invalid, with normal inflammation index, negative results for rheumatic factor (RF) and human leukocyte antigen (HLA)-B27, and normal erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP). She had hyphosphatemia, normal serum calcium, 1,25-(OH)2-D3 reduction, elevated alkaline phosphatase (ALP) and normal parathyroid hormone (PTH), however with elevated urinary phosphorus. Finally, the medial thigh nodule was found in the subcutaneous of her inner leg by careful examination and imaging scans including B-ultrasound and PET/CT. The final pathology confirmed that the nodule was phosphate urinary mesenchymal tumors. After the tumor was removed, the patient was treated with anti-osteoporosis and phosphorus supplementation. The symptoms of bone pain and muscle weakness were alleviated, and hypophosphatemia was corrected. It was confirmed that the patient had low-phosphorus osteomalacia due to tumor. Tumor-induced hypophosphatemia osteomalacia (TIO) was a rare paraneoplastic syndrome which was caused by excessive phosphorus excretion induced by the tumor, and was thus categorized as an acquired hypophosphatemic osteomalacia. TIO had an occult onset and was associated with a high rate of misdiagnosis, although TIO has some typical clinical features. Early diagnosis, correctly positioning of the tumor, and surgical resection can achieve good outcomes.


Assuntos
Doenças do Sistema Endócrino , Hipofosfatemia , Neoplasias de Tecido Conjuntivo , Osteomalacia , Adulto , Feminino , Humanos , Tomografia Computadorizada com Tomografia por Emissão de Pósitrons
6.
PLoS One ; 14(12): e0218653, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31856197

RESUMO

Chinese hamster ovary (CHO) cells are a common tool utilized in bioproduction and directed genome engineering of CHO cells is of great interest to enhance recombinant cell lines. Until recently, this focus has been challenged by a lack of efficacious, high throughput, and low-cost gene editing modalities and screening methods. In this work, we demonstrate an improved method for gene editing in CHO cells using CRISPR RNPs and characterize the endpoints of Cas9 and ZFN mediated genetic engineering. Furthermore, we validate sequence decomposition as a cost effective, rapid, and accurate method for assessing mutants and eliminating non-clonal CHO populations using only capillary sequencing.

7.
Zhonghua Shao Shang Za Zhi ; 35(12): 839-841, 2019 Dec 20.
Artigo em Chinês | MEDLINE | ID: mdl-31877604

RESUMO

Scar formation is the abnormal healing process of skin after being damaged. The mechanism of scar formation is not clear, and many studies have shown that it is affected by many factors. Based on the over deposition of collagen in scars, many researchers have carried out studies on the mechanism, pathological manifestation, and treatment method of scars. In the treatment aspect of scar, the combination of traditional and new treatment methods has been well accepted and achieved good results. To understand the new advances of scar research and combine it with clinical treatment transformation could lead to the development of more effective prophylactic and therapeutic strategies for scar treatment in the future.


Assuntos
Cicatriz , Pele , Colágeno , Humanos
8.
Neoplasma ; 2019 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-31847526

RESUMO

Pancreatic cancer (PC) is a complex and multifactorial human malignancy with a low survival rate. Tumor protein D52 (TPD52) was abnormally expressed in several cancers and participated in tumorigenesis. However, the oncogenic effect of TPD52 on PC remains unknown. In the present study, after transfecting with NC or sh-TPD52 into AsPC-1 and PANC-1 cells, the CCK-8 assay, Hoechst staining, western blot, transwell assay, flow cytometry were used to examine the cell proliferation, migration, invasion and apoptosis. qRT-PCR results confirmed that the expression of TPD52 was significantly increased in PC cells, especially AsPC-1 and PANC-1 cells. The present study revealed that silencing of TPD52 significantly suppressed the proliferation, migration and invasion, but induced apoptosis of AsPC-1 and PANC-1 cells in vitro by dephosphorylating Akt at Ser473. Conversely, SC79, an Akt activator, could partially reverse the anti-metastatic effects of sh-TPD52, accompanied by the reactivating of Akt pathway. Additional in vivo studies are warranted to elucidate that knockdown of TPD52 could inhibit tumor growth in PC mice models. These findings suggested that TPD52 might be a novel therapeutic target for PC treatment.

9.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 31(5): 555-559, 2019 Jul 24.
Artigo em Chinês | MEDLINE | ID: mdl-31713395

RESUMO

Exosomes are tiny vesicles secreted by most endogenous cells, and the extracellular vesicles (EVs) are specifically secreted by cells. Recently, it was found that exosomes contain a large quantity of important substances such as proteins, nucleic acids, and lipids, which play important roles in material exchange and information transmission in cell-cell communication, and in modulating the immune response, metabolism, and expansion, metastasis, and drug resistance of tumors. This paper summarizes the recent researches on exosomes in parasites and parasitic diseases and hopes to be helpful for improving the researches of parasites and parasitic diseases.


Assuntos
Exossomos , Vesículas Extracelulares , Parasitos , Doenças Parasitárias , Animais , Vesículas Extracelulares/fisiologia , Interações Hospedeiro-Parasita/fisiologia , Parasitos/citologia , Doenças Parasitárias/parasitologia , Pesquisa/tendências
10.
Artigo em Chinês | MEDLINE | ID: mdl-31594134

RESUMO

Objective: To establish a CT image radiomics-based prediction model for the differential diagnosis of silicosis and tuberculosis nodules. Methods: A total of 53 patients with silicosis and 89 patients with tuberculosis who underwent routine CT scans in Suzhou Fifth People's Hospital from January to August, 2018 were enrolled in this study. AK/ITK software was used to segment the images to obtain 139 silicosis lesions and 119 tuberculosis lesions. For each lesion image, 396 features were extracted, and feature dimension reduction was applied to select the most characteristic feature subset. Support vector machine (SVM) , feedforward back propagation neural network (FNN-BP) , and random forest (RF) were implemented using R software (Rstudio V1.1.463) , and the algorithm that achieved the largest area under of the receiver operating characteristic (ROC) curve (AUC) was selected as the final prediction model. Results: RF was the best prediction model for the differential diagnosis of silicosis and tuberculosis nodules, with an accuracy of 83.1%, a sensitivity of 0.76, a specificity of 0.9, and an AUC of 0.917 (95% confidence interval: 0.8431-0.9758) . RF had a significantly larger AUC than SVM and FNN-BP (P<0.05) . Conclusion: CT image-based RF prediction model can be used to differentially diagnose silicosis and tuberculosis nodules.


Assuntos
Silicose/diagnóstico por imagem , Tuberculose/diagnóstico por imagem , Diagnóstico Diferencial , Humanos , Interpretação de Imagem Assistida por Computador , Modelos Teóricos , Curva ROC , Máquina de Vetores de Suporte , Tomografia Computadorizada por Raios X
11.
Eur Rev Med Pharmacol Sci ; 23(18): 8000-8010, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31599425

RESUMO

OBJECTIVE: Long noncoding RNAs (lncRNAs) have drawn increasing attention due to their critical roles in various diseases, including melanoma. In this study, we aimed to explore the potential roles and underlying mechanisms of Small nucleolar RNA host gene 17 (SNHG17) in the modulation of melanoma progression. PATIENTS AND METHODS: The levels of SNHG17 in melanoma tissues and cells were determined using RT-PCR. The clinical significance of SNHG17 in melanoma patients was analyzed using Chi-square tests, Kaplan-Meier methods, and multivariate assays. MTT assays, transwell assay, and flow cytometry were carried out to explore the biological functions of SNHG17. The biological mechanism underlying up-regulation of SNHG17 was explored using ChIP analysis and luciferase reporter assays. The related proteins of the PI3K-AKT pathway were determined by Western blot. RESULTS: High expressions of SNHG17 were observed in both melanoma tissues and cells. Up-regulation of SNHG17 in melanoma patients was associated with lymph node metastasis and tumor stage. Survival assays revealed that those patients with high SNHG17 expression had significantly shorter survival time. SNHG17 was also confirmed to be independently associated with overall survival of melanoma patients. Functional studies confirmed that the proliferation, migration, and invasion of melanoma cells were noticeably reduced by the down-regulation of SNHG17. Mechanistically, the up-regulation of SNHG17 was induced by STAT3. We also found that knockdown of SNHG17 resulted in the remarkable diminution in the phosphorylation levels of PI3K and AKT, suggesting that the activity of the PI3K-AKT pathway was suppressed. CONCLUSIONS: STAT3-induced upregulation of SNHG17 contributed to the progression of melanoma by promoting the PI3K-AKT signaling.

12.
Clin Transl Oncol ; 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31571151

RESUMO

PURPOSE: Cetuximab (CTX) has been used to treat metastatic colorectal cancer (mCRC) with wild-type (wt) RAS and BRAF genes. Meanwhile HER2 amplification reportedly denoted CTX-resistant mCRC tumors. We investigated whether monitoring of HER2 amplification in circulating DNA allowed early detection of mCRC progression and CTX resistance. METHODS: We analyzed HER2 amplification in circulating DNA at 8-week intervals using ddPCR from 36 patients with RASwt/BRAFwt mCRC, who progressed after CTX treatments between July 2015 and January 2018. RESULTS: Of the 36 patients, 5 (13.9%) exhibited dynamic fluctuations of HER2 amplification in plasma in the course of CTX treatment, of whom 2 were positive for HER2 amplification in matched tumor specimens at baseline (per FISH). All 5 primary sites were left side: 3 rectums and 2 descending colon. HER2 ratio fluctuations in circulating DNA not only reflected changes in tumor volume, but their obvious increases presaged CT-documented progress by an average lead time of 2 months. Interestingly, progression-free survival did not significantly differ between these 5 patients and those without HER2 amplification (HR 1.06, 95% CI 0.40-2.77, P = 0.909). CONCLUSION: Plasma HER2 amplification detected by ddPCR changed over time and predicted resistance to CTX, by an average lead time of 2 months. Further study is needed to validate our findings.

13.
Acta Virol ; 63(3): 333-337, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31507201

RESUMO

Molluscum contagiosum is a common, self-limiting infectious disease of the skin caused by molluscum contagiosum virus (MCV). The disease primarily affects children, sexually active adults, and immunocompromised individuals. Transmission of the virus occurs by direct skin contact. Therefore, the virus is usually detected in the skin and genitals of patients. However, the diagnosis of intracranial infection by the virus is difficult if the skin/mucosa lessons are atypical or absent, and the presence of the virus in the cerebrospinal fluid has not been reported. We report a very rare case of intracranial infection by molluscum contagiosum virus. A 25-year-old girl was admitted to our hospital due to severe headache but no fever or other symptoms. Upon examination, some small flesh-colored flattened papules on both arms were noticed. Blood tests showed slightly reduced levels of CD3 and CD4 T lymphocytes. Three-dimensional time-of-flight magnetic resonance angiography (3D-TOF-MRA) and head magnetic resonance (MR) were both normal. Lumbar puncture was performed, and metagenomic sequencing was applied to the spinal fluid. The unique sequences of the molluscum contagiosum virus were identified in the fluid. The patient was then diagnosed with intracranial molluscum contagiosum virus infection. No special treatment was given. The headache gradually disappeared, and the patient was discharged. During our quarterly follow-up, the girl appeared normal, and her skin lesions disappeared. However, her CD3 and CD4 T lymphocyte counts were still slightly lower than the normal level. Our case shows that the application of metagenomic sequencing to cerebrospinal fluid is a sensitive and powerful means to detect pathogens causing intracranial infection. Keywords: Molluscum contagiosum; intracranial infection; metagenomics sequencing.


Assuntos
Metagenômica , Molusco Contagioso , Vírus do Molusco Contagioso , Adulto , Linfócitos T CD4-Positivos/citologia , Feminino , Humanos , Contagem de Linfócitos , Molusco Contagioso/líquido cefalorraquidiano , Molusco Contagioso/diagnóstico , Molusco Contagioso/imunologia , Vírus do Molusco Contagioso/genética , Pele/virologia
14.
Eur Rev Med Pharmacol Sci ; 23(17): 7694-7702, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31539162

RESUMO

OBJECTIVE: The paper aims to explore the application of dexmedetomidine combined with dezocine in thoracoscopic radical resection of lung cancer and its effect on the awakening quality. PATIENTS AND METHODS: 122 patients undergoing thoracoscopic radical resection of lung cancer in The Affiliated Hospital of Qingdao University from April 2009 to January 2012 were selected as the subjects of the study. Among them, 68 patients were anesthetized with dexmedetomidine combined with dezocine as a study group, 54 patients with midazolam combined with fentanyl as a control group. The onset of anesthetic, operation time, awakening time, extubation time, and recovery time was compared. The mean arterial pressure (MAP), central venous pressure (CVP), and heart rate (HR) were compared before anesthesia (t0), at extubation (t1), 10 min after extubation (t2), and when patients left anesthesia recovery room (t3). The postoperative sedation score (Ramsay), modified the objective pain score (MOPS), and the pediatric anesthesia emergence delirium (PAED) score were compared at the time of the postoperative awakening (b1), 30 min after awakening (b2), 1 hour after awakening (b3), and 3 hours after awakening (b4). RESULTS: There was no significant difference in MAP, CVP, and HR between the study group and the control group at t0 (p > 0.05). The scores of PAED at b3 and b4 in the study group were lower than those in the control group (p < 0.05). CONCLUSIONS: The anesthesia effect of dexmedetomidine combined with dezocine in thoracoscopic radical resection of lung cancer is better and safer than other drugs, and it can produce good sedation and analgesic effect.

15.
Fa Yi Xue Za Zhi ; 35(4): 402-405, 2019 Aug.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-31532146

RESUMO

Abstract: Objective To research the correlation between the visual acuity ratio and pattern reversal visual evoked potential (PRVEP) P100 waveform amplitude ratio of both eyes. Methods Forty-seven volunteers were selected, and the visual chart visual acuity of both eyes was measured. The visual acuity ratio of the eye with poor vision to the eye with better vision was calculated by five grade notation method. The amplitudes of P100 waveforms of both eyes were recorded respectively by using black-and-white checkerboard PRVEP and chosing 1°, 15' stimulating visual angle, and the ratio of amplitudes between the two eyes was also calculated. SPSS 20.0 software was used to analyze the correlation between the visual acuity ratio and the ratio of P100 waveform amplitudes between the two eyes. Return test and linear regression analysis with the binocular ratio of P100 waveform amplitudes as the independent variable (x) and the binocular visual acuity ratio as the dependent variable (y) were made. Results There was a positive correlation between the binocular visual acuity ratio and the ratio of P100 waveform amplitudes under 15' stimulating visual angle (Pearson correlation coefficient was 0.62, P=0.000). The fitting linear regression equation was y=0.090 x+0.846 (F=20.954, P=0.000). There was no significant correlation between the binocular ratio of visual acuity and the binocular ratio of P100 waveform amplitudes under 1° stimulating visual angle (P>0.05). Results of return test showed that there was no statistical significance in the difference between visual acuity estimated by equation and actual detected visual acuity. Conclusion In forensic appraisal of monocular injury, fitting linear regression equation of binocular visual acuity ratio and the binocular ratio of P100 waveform amplitudes under 15' stimulating visual angle, is helpful for visual acuity level estimation of the injured eye to some extent.


Assuntos
Potenciais Evocados Visuais , Olho/fisiopatologia , Visão Ocular , Acuidade Visual , Humanos , Análise de Regressão
16.
Artigo em Chinês | MEDLINE | ID: mdl-31495110

RESUMO

Objective: To analyze Nursing Occupational Risk, the relationship between resilience and job performance, and investigate the mediating effects of resilience on Nursing Occupational Risk and job performance. Methods: 406 nurses from33 clinical departments of Chengdu first-class hospital were recruited by randomsampling method. Thier Nursing Occupational Risk, resilience and job performance were interviewed by nursing occupational risk assessment questionnaire, the Scale of medical staff resilience and the job performance questionnaire, and analyze the relationship between variables. Results: The clinical nurses' resilience is 82.98±10.05, the clinical nurses' job performance is 145.79±23.55; here was a positive correlation between clinical nurses' resilience and job performance (P<0.05) ; three variable of resilience can explain 33.2% of the variation of clinical nurses job performance; in the highest the Nursing Occupational risk, resilience served to mediate the relationship between Nursing Occupational Risk and job performance, which was 56.77% of the total effect respective. Conclusion: resilience are closely related to job performance. Resilience plays mediating role in the relationships between Nursing Occupational Risk and job performance, three dimensions of resilience can predict nurses' job performance. Improving the resilience of nurses can effectively improve their job performance.


Assuntos
Adaptação Psicológica , Satisfação no Emprego , Enfermeiras e Enfermeiros/psicologia , Desempenho Profissional , China , Humanos , Inquéritos e Questionários
17.
Zhonghua Xin Xue Guan Bing Za Zhi ; 47(8): 614-621, 2019 Aug 24.
Artigo em Chinês | MEDLINE | ID: mdl-31434432

RESUMO

Objective: To investigate the effects and potential mechanisms of tolvaptan on chronic intermittent hypoxia (CIH)-induced atrial remodeling in rats. Methods: A total of 45 Sprague-Dawley rats were divided into 3 groups by the random number table: control group, CIH group (6 h/d for 30 days), CIH plus tolvaptan group (8 mg·kg(-1)·d(-1) per gavage for 30 days). Echocardiography examination was performed after 30 days. Thereafter, 5 rats were randomly chosen for histology evaluation, 5 for molecular biological examinations and another 5 rats underwent isolated heart electrophysiology study in each group. Protein and mRNA expression levels of miRNA-21, Spry1, PTEN, ERK/p-ERK, MMP-9, PI3K, AKT/p-AKT were detected. Results: Compared to the rats in control group, rats in the CIH group showed higher atrial interstitial collagen deposition (P<0.001), increased atrial fibrillation inducibility (P=0.022). The results of immunohistochemistry staining showed that the mean optical density (MOD) of ERK, p-ERK and MMP-9 were significantly increased (all P<0.05), the MOD of Spry1 and PTEN were significantly decreased (both P<0.05), above changes could be significantly reversed by cotreatment with tolvaptan. No significant differences were detected in PI3K and AKT among the three groups (P>0.05). In addition, compared with rats in control group, mRNA levels of miRNA-21, MMP-9, PI3K, AKT, and protein levels of ERK, p-ERK, MMP-9 were significantly increased in CIH group(all P<0.05), whereas protein levels of Spry1, PI3K, p-AKT were significantly decreased (all P<0.05). Above changes could be significantly attenuated. Conclusions: CIH induces significant atrial remodeling in this rat model, which can be attenuated by tolvaptan possibly through modulating miRNA-21/Spry1/ERK/MMP-9 and miRNA-21/PTEN/PI3K/AKT signaling pathways.


Assuntos
Remodelamento Atrial , Animais , Hipóxia , MicroRNAs , Fosfatidilinositol 3-Quinases , Ratos , Ratos Sprague-Dawley , Tolvaptan
18.
Carbohydr Polym ; 223: 115117, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31427014

RESUMO

Novel, portable, probe-free, liquid cellulose biosensor was explored via a facile one-pot process for Cu2+ ions detection by naked eyes. The as-prepared biosensor displayed specific recognition towards Cu2+ ions in aqueous solution, even in complex urine and serum samples within 10 s, showing fast response characteristic. There is barely any interference on the Cu2+ ions detection by other metal ions or other complex substances in urine and serum, and the corresponding detection limit is as low as 1.185, 1.9309 and 1.9154 ppm, respectively. Moreover, the cellulose biosensor could act as an excellent candidate to sense Cu2+ ions in biological samples including urine and serum from SD rats with satisfying accuracies and biocompatibility. This study exploits a potential application of cellulose in biosensing and monitoring related to Cu2+ ions.

19.
Eur Rev Med Pharmacol Sci ; 23(15): 6387-6393, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31378876

RESUMO

OBJECTIVE: To study the expression and significance of micro ribonucleic acid (miR)-126 and vascular endothelial growth factor (VEGF) in the vitreous body and proliferative membrane tissues of affected eyes and plasma in proliferative diabetic retinopathy (PDR). PATIENTS AND METHODS: A total of 50 PDR patients admitted to our hospital from January 2017 to January 2018 were collected, including 17 cases in stage IV, 20 cases in stage V, and 13 cases in stage VI according to the DR staging criteria. Another 30 patients with an idiopathic macular hole were selected as control group. After admission, the examinations were performed, venous blood was drawn, and vitrectomy was performed, during which the vitreous body and proliferative membrane tissues were taken. The plasma VEGF content was detected via enzyme-linked immunosorbent assay (ELISA). The VEGF protein expression level was detected via Western blotting in vitreous body and proliferative membrane tissues. Moreover, the miR-126 expression was detected via quantitative polymerase chain reaction (qPCR). RESULTS: In the vitreous body tissues, proliferative membrane tissues and plasma, the expression level of miR-126 was significantly lower in control group than that in stage IV, V, and VI groups (p<0.05). The miR-126 expression was significantly lower in stage IV group than that in stage V and VI groups (p<0.05), meanwhile, miR-126 expression was significantly lower in stage V group than that in stage VI group (p<0.05). In the vitreous body tissues, proliferative membrane tissues and plasma, the VEGF mRNA and protein expression levels were significantly higher in control group than those in stage IV, V, and VI groups (p<0.05). The VEGF expressions were significantly higher in stage IV group than those in stage V and VI groups (p<0.05). In addition, VEGF expressions were significantly higher in stage V group than those in stage VI group (p<0.05). Furthermore, the VEGF expression level was negatively correlated with the miR-126 expression level. CONCLUSIONS: We found that the abnormally high expression of miR-126 negatively regulates VEGF, which may be one of the important mechanisms of occurrence of PDR. The miR-126 and VEGF content can serve as important predictors for the condition of PDR.

20.
Artigo em Inglês | MEDLINE | ID: mdl-31334767

RESUMO

BACKGROUND: Substandard and falsified (SF) medical products are a major danger to public health. They affect every region of the world, and have been identified in all major therapeutic categories. Studies from medicine, pharmacology, law and public health dominate this research area with a focus on the supply side. However, the spread of SF medical products cannot be fully understood without information about the demand side or a sociocultural perspective on market formation. The aim of this short report is to present findings from a pilot study that examines the attitudes of the Swedish public regarding consumption of medicines. METHODS: We conducted a pilot survey in 2016 'Where and how do you buy medicines?' using LimeSurvey, an open-source online survey software. In total 155 respondents completed the survey. RESULTS: The majority of respondents turn to doctors within healthcare for prescription-only medicines (POM). Simultaneously, some respondents would consider buying POMs without prior contact with experts even if medicines may come from unsafe sources. CONCLUSIONS: There is a tendency that people move away from formal healthcare towards an unregulated market. In parallel, people's approach to doctors becomes more personalized and pragmatic than in former patriarchal relationships. Risk becomes a negotiable concept.

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