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1.
Artigo em Inglês | MEDLINE | ID: mdl-32012486

RESUMO

Biomaterial-based therapy that can restore annulus fibrosus (AF) function in early stage and promote endogenous repair of AF tissues is a promising approach for AF tissue repair. In this study, we established a genipin-crosslinked decellularized AF hydrogels (g-DAF-G) that are injectable and could manifest better in situ formability than noncrosslinked decellularized AF hydrogel, while preserving the capacity of directing differentiation of human bone mesenchymal stem cells (hBMSCs) towards AF cells. Hematoxylin and eosin staining, 4',6-diamidino-2-phenylindole staining, and so forth showed that the majority of cellular components were removed, whereas extracellular matrix and microstructure were largely preserved. The storage modulus increased from 465.5 ± 9.4 Pa to 3.29 ± 0.24 MPa after 0.02% genipin crosslinking of decellularized AF hydrogels (DAF-G) to form g-DAF-G. AF-specific genes (COL1A1, COL5A1, TNMD, IBSP, FBLN1) were significantly higher in DAF-G and g-DAF-G groups than that in control group after 21 days of culturing. g-DAF-G significantly restored nucleus pulposus water content and preserved intervertebral structure in vivo. Summarily, we produced a novel AF regeneration biomaterial, g-DAF-G, which exhibited well biocompatibility, great bioactivity, and much higher mechanical strength than DAF-G. This study will provide an easy and fast therapeutic alternative to repair AF injuries or tears.

2.
J Asian Nat Prod Res ; : 1-12, 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32009450

RESUMO

Three series of sulfonate derivatives of paeonol were synthesized and screened in vitro for their anti-oomycete activity against P. capsici, respectively. Among all the compounds, 4m displayed the best promising and pronounced anti-oomycete activity against P. capsici than zoxamide, with the EC50 values of 24.51 and 26.87 mg/L, respectively. The results show that acetyl and 4-OCH3 are two necessary groups. The existence of these two sites is closely related to the anti-oomycete activity. Relatively speaking, hydroxyl group is well tolerated, and the results showed that after modification of hydroxyl group with sulfonyl, the anti-oomycete activity was significantly increased.[Formula: see text].

3.
Clin Rheumatol ; 2020 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-32036586

RESUMO

OBJECTIVE: The diagnosis of relapsing polychondritis (RP) is often mistaken or delayed. In this retrospective cohort, we aimed to unveil the causes responsible for such phenomenon, to determine the associated factors, and to compare diagnosis in clinical settings with the current diagnostic criteria. METHOD: Eighty-seven RP patients followed-up by rheumatologists from January 1, 2008, to October 31, 2018, were retrospectively analyzed. RESULTS: A total of 50 male and 37 female patients were included with a mean age of 45.9 ± 14.5 years. Ninety-three percent were initially admitted by non-rheumatologic specialists .Twenty-eight percent were correctly diagnosed, while 72% were misdiagnosed at the first visits, all by non-rheumatologic specialists. Patients admitted by non-rheumatologic specialists had increased odds of misdiagnosis (odds ratio [OR] = 1.3, 95% confidence interval [95% CI] 1.1-1.7, P = 0.000). Fifty-seven (65.5%) patients did not meet with Michet or Damiani criteria, with 16 (18.4%) patients diagnosed as partial RP and 41( 47.1%) patients diagnosed as limited RP. CONCLUSIONS: Incorrect and delayed diagnosis of RP is common in our cohort, and insufficient awareness of the disease in non-rheumatologic specialists at least partially contributes to this. It is imperative to revise the current criteria for early diagnosis.Key Points• Diagnosing relapsing polychondritis (RP) in early stage remains challenging after all these years, especially among non-rheumatologic specialists, indicating the importance of teaching non-rheumatologic specialists to improve their understanding of this rare disease.• Many RP patients did not fully meet with the current criteria, suggesting that revision of the current criteria is imperative for early diagnosis of this rare disease.

4.
Brief Bioinform ; 2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-32003790

RESUMO

Moonlighting proteins provide more options for cells to execute multiple functions without increasing the genome and transcriptome complexity. Although there have long been calls for computational methods for the prediction of moonlighting proteins, no method has been designed for determining moonlighting long noncoding ribonucleicacidz (RNAs) (mlncRNAs). Previously, we developed an algorithm MoonFinder for the identification of mlncRNAs at the genome level based on the functional annotation and interactome data of lncRNAs and proteins. Here, we update MoonFinder to MoonFinder v2.0 by providing an extensive framework for the detection of protein modules and the establishment of RNA-module associations in human. A novel measure, moonlighting coefficient, was also proposed to assess the confidence of an ncRNA acting in a moonlighting manner. Moreover, we explored the expression characteristics of mlncRNAs in sepsis, in which we found that mlncRNAs tend to be upregulated and differentially expressed. Interestingly, the mlncRNAs are mutually exclusive in terms of coexpression when compared to the other lncRNAs. Overall, MoonFinder v2.0 is dedicated to the prediction of human mlncRNAs and thus bears great promise to serve as a valuable R package for worldwide research communities (https://cran.r-project.org/web/packages/MoonFinder/index.html). Also, our analyses provide the first attempt to characterize mlncRNA expression and coexpression properties in adult sepsis patients, which will facilitate the understanding of the interaction and expression patterns of mlncRNAs.

5.
ACS Appl Mater Interfaces ; 12(5): 5909-5919, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31944646

RESUMO

Effective hosts of sulfur are essential for the application of lithium-sulfur batteries. However, various refined nanomaterials or carbon-based hosts possess low density, high surface area, and large porosity, leading to undesirable reduction on both gravimetric and volumetric energy densities. Herein, spherical metal oxides with high tap density are introduced as carbon-free hosts of sulfur for the first time. The ternary oxides show a superior synergistic effect of adsorption and electrocatalytic conversion of soluble intermediate polysulfides. Besides, oxide microspheres can build stable conductive frameworks and open channels in porous electrodes for fast transport of electrons and active diffusion of electrolyte. Such a synergistic effect and unique structural feature of porous electrodes are favorable for achieving good utilization and stable cycle performance of the sulfur cathode. Typically, the S/LiNi0.8Co0.1Mn0.1O2 composite exhibits good cycle stability with a low capacity decay rate (0.057% per cycle) during 500 cycles at 0.1 C. Importantly, due to the high tap density (1.81 g cm-3), the S/LiNi0.8Co0.1Mn0.1O2 composite delivers a larger volumetric capacity (1601.9 mAh cm-3-composite), almost 2.3 times of S/carbon composite (689.4 mAh cm-3-composite). Therefore, this work provides a feasible strategy to reach long life and high volumetric capacity of cathode based on metal oxides as sulfur hosts.

6.
7.
Bioorg Chem ; 95: 103502, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31901756

RESUMO

Seven new diterpenoids, including four ent-kaurane-type pierisentkaurans B-E (1-4), one 4,5-seco- ent-kaurane-type pierisentkauran F (5), two leucothane-type 3ß,7α,14ß-trihydroxy-leucoth-10(20),15-dien-5-one (6) and 10α,16α-dihydroxy-leucoth-5-one (7), along with three known diterpenoids ent-kaurane-type 16α-dihydroxy-6-oxo-ent-kauran-18-oic-acid (8), kalmane-type rhodomollein XXIII (9), and grayanane-type pierisformosoid J (10), were isolated from the roots of Pieris formosa. Their structures with absolute configurations were determined by a series of spectroscopic methods and electronic circular dichroism (ECD) calculations. Compounds 2 and 7 displayed weak analgesic activity at a dose of 5.0 mg/kg (i.p.) compared to the vehicle tests (p < 0.05) in an acetic acid-induced writhing test. At a dose of 0.5 mg/mL, compounds 3 and 7 showed antifeedant activity against Plutella xylostella larvae with inhibition ratios of 27.1% and 52.5%, respectively.

8.
Molecules ; 25(3)2020 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-31991928

RESUMO

A highly efficient sulfonylation of para-quinone methides with sulfonyl hydrazines in water has been developed on the basis of the mode involving a tetrabutyl ammonium bromide (TBAB)-promoted sulfa-1,6-conjugated addition pathway. This reaction provides a green and sustainable method to synthesize various unsymmetrical diarylmethyl sulfones, showing good functional group tolerance, scalability, and regioselectivity. Further transformation of the resulting diarylmethyl sulfones provides an efficient route to some functionalized molecules.

9.
Plant Biotechnol J ; 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31945255

RESUMO

Amino acid transport via phloem is one of the major source-to-sink nitrogen translocation pathways in most plant species. Amino acid permeases (AAPs) play essential roles in amino acid transport between plant cells and subsequent phloem or seed loading. In this study, a soybean AAP gene, annotated as GmAAP6a, was cloned and demonstrated to be significantly induced by nitrogen starvation. Histochemical staining of GmAAP6a:GmAAP6a-GUS transgenic soybean revealed that GmAAP6a is predominantly expressed in phloem and xylem parenchyma cells. Growth and transport studies using toxic amino acid analogs or single amino acids as a sole nitrogen source suggest that GmAAP6a can selectively absorb and transport neutral and acidic amino acids. Overexpression of GmAAP6a in Arabidopsis and soybean resulted in elevated tolerance to nitrogen limitation. Furthermore, the source-to-sink transfer of amino acids in the transgenic soybean was markedly improved under low nitrogen conditions. At the vegetative stage, GmAAP6a-overexpressing soybean showed significantly increased nitrogen export from source cotyledons and simultaneously enhanced nitrogen import into sink primary leaves. At the reproductive stage, nitrogen import into seeds was greatly enhanced under both sufficient and limited nitrogen conditions. Collectively, our results imply that overexpression of GmAAP6a enhances nitrogen stress tolerance and source-to-sink transport and improves seed quality in soybean. Co-expression of GmAAP6a with genes specialized in source nitrogen recycling and seed loading may represent an interesting application potential in breeding.

10.
FASEB J ; 34(1): 1169-1181, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31914631

RESUMO

BHLHE40, a member of the basic helix-loop-helix transcription factor family, has been reported to play an important role in inflammatory diseases. However, the regulation and function of BHLHE40 in Helicobacter pylori (H pylori)-associated gastritis is unknown. We observed that gastric BHLHE40 was significantly elevated in patients and mice with H pylori infection. Then, we demonstrate that H pylori-infected GECs express BHLHE40 via cagA-ERK pathway. BHLHE40 translocates to cell nucleus, and then binds to cagA protein-activated p-STAT3 (Tyr705). The complex increases chemotactic factor CXCL12 expression (production). Release of CXCL12 from GECs fosters CD4+ T cell infiltration in the gastric mucosa. Our results identify the cagA-BHLHE40-CXCL12 axis that contributes to inflammatory response in gastric mucosa during H pylori infection.

11.
Artigo em Inglês | MEDLINE | ID: mdl-31985371

RESUMO

BACKGROUND: Plant secondary metabolites play an important role in novel insecticide discovery for their unique sources and potential target sites. Paeonol, the main phenolic components in Moutan Cortex, is recognized as a safe and potent botanical insecticide to many insects. The structural modification of paeonol into phenylsulfonylhydrazone derivatives is a potential approach for the development of novel insecticides, which showed more toxicity than paeonol. However, there are no reports on the insecticidal activity of paeonol-based phenylsulfonylhydrazone derivatives to control Mythimna separata. METHODS: We always endeavor to discover biorational natural products-based insecticides, 12 novel paeonol-based phenylsulfonylhydrazone derivatives have been successfully perpared by structural modification of paeonol, and evaluated the insecticidal activity against M. separata by the leaf-dipping method at the concentration of 1 mg/mL. RESULTS: Insecticidal activity revealed that, out of 12 title compounds, derivatives 5c and 5f displayed the best against M. separate with the FMR both of 53.6% than toosendanin (FMR = 50.0%). CONCLUSION: It suggested that for the paeonol-based phenylsulfonylhydrazone series derivatives, the proper substituent of arylsulfonyl R at the hydroxyl position of paeonol was very important for their insecticidal activity. These preliminary results will pave the way for further modification of paeonol in the development of potential new insecticides.

12.
Nanotechnology ; 31(11): 115703, 2020 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-31778980

RESUMO

Carbon nanotubes are outstanding reinforcements owing to their unparallel strength, while their effects on the copper nanowire are still not fully understood, hampering their broad applications. Herein, we have investigated the tensile behaviors of the nanocomposite-wire of carbon nanotube-copper using molecular dynamic simulations. For the nanocomposite, both the coated and embedded carbon nanotubes increase the Young's modulus, fracture stress and toughness of the copper nanowire. A reinforcement of over fivefold in both yield strength (5.3 times) and toughness (5.1 times) has been achieved when the carbon nanotubes are coated on the copper nanowires, as well as 1.7 times in the Young's modulus. Higher temperatures and lower loading rates reduce the reinforcement.

13.
Plant Physiol Biochem ; 146: 294-302, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31783205

RESUMO

Brassica napus (rapeseed) serves as a main source of edible oil, and the oil's quality is mainly determined by the relative proportions of fatty acids. A high oleic acid concentration in B. napus oil increases its shelf life and oxidative stability. Therefore, attaining a high oleic acid concentration is necessary to enhance the nutritional quality of rapeseed oil. Here, an association study of candidate genes was conducted using a population of 324 genetically diverse rapeseed accessions, and several loci related to oleic acid content were identified. Furthermore, these loci were functionally characterized in Saccharomyces cerevisiae to assess their functions, and the promising candidate loci were validated using single nucleotide polymorphic markers in an independent inbred population. The results increased our understanding of fatty acid metabolism in B. napus. Moreover, these findings may assist in marker-based breeding efforts to improve the fatty acid composition and quality of B. napus oil.

14.
Spectrochim Acta A Mol Biomol Spectrosc ; 229: 117941, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31884400

RESUMO

Three diverse supramolecular compounds, {[3-pmpmd]}n(1), {H2[3-pmpmd]·2NO3-}n(2), {H2[3-pmpmd]·2tbb}n(3), (where 3-pmpmd was N,N'-bis(3-pyridylmethyl)pyromellitic diimide; tbb was tertiary butyl benzoic acid) were synthesized and characterized by elemental analyses, IR spectroscopy, thermogravimetric analyses, and X-ray single-crystal structure analysis. The co-crystal anions affect their structural diversity. Compound 1 is a 1D stairway chain structure by perpendicular π⋯π interactions of pyromellitic diimide and the neighboring pyridine rings. Compound 2 is a typical 1D ladder chain structure by hydrogen bond of nitrate arranged in a parallel array. Compound 3 is a 1D zigzag chain structure of one 3-pmpmd and two tbb by parallel π⋯π interactions and hydrogen bond interactions. Thermal stabilities and fluorescence properties of all compounds were investigated. The solution of compound 2 in DMF can fluorescence sense for iodide ions by remarkably quenching fluorescence intensity.

15.
Biomed Pharmacother ; 121: 109617, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31810139

RESUMO

Bone metastasis of breast cancer causes severe skeletal-related events and poor prognosis. Wensheng Zhuanggu Formula (WSZG), a traditional Chinese prescription, is used to adjunctively treat breast cancer bone metastases in clinical practice. This study was undertaken to investigate the antibone-metastatic activities and mechanisms of WSZG extract by evaluating the effect of this formula on the cross-talk between bone marrow-derived mesenchymal stem cells (BMSCs) and breast cancer cells in triggering epithelial-mesenchymal transition (EMT) in vivo and in vitro. The results demonstrated that BMSCs might enhance the invasive and metastatic potentials of breast cancer cells as a consequence of EMT induction through direct cell-to-cell contact. WSZG treatment remarkably suppressed motility, invasion, EMT-related gene, and protein markers in BMSC-conditioned breast cancer cells and ameliorated bone metastases and damages in nude mice following co-injection of BMSCs and MDA-MB-231BO breast cancer cells. Further investigation showed that the transforming growth factor-ß1 (TGF-ß1)/Smads pathway was an important mechanism enabling BMSCs to induce EMT occurrence of breast cancer cells. WSZG treatment reversed BMSC-induced EMT by downregulating TGF-ß1/Smads signaling. Thus, WSZG extracts may be regarded as a potential antibone-metastatic agent for breast cancer therapy.

16.
Mol Biol Rep ; 47(2): 1257-1264, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31853767

RESUMO

Leucine aminopeptidase 3 (LAP3) is an important proteolytic enzyme that catalyzes the hydrolysis of leucine residues from the amino termini of protein or peptide substrates and plays a critical role in protein metabolism and growth. In the present study, we investigated the full-length cDNA sequence of the LAP3 gene in Sinonovacula constricta (ScLAP3) using expressed sequence tags and rapid amplification of cDNA ends. The full-length ScLAP3 cDNA was 2885 bp, with a 1560 bp open reading frame encoding 519 amino acids. Sequence analysis revealed that ScLAP3 shared 70.9% identity with LAP3 from the blood clam Tegillarca granosa and 62.0-68.0% with other species. ScLAP3 was expressed in all six tested tissues, with significantly higher expression levels in the foot compared with mantle, adductor muscle, liver, gills, and siphon tissues in adults (P < 0.01). In the eight developmental stages, ScLAP3 expression gradually increased, with significantly higher levels in D-shaped larvae compared with other developmental stages (P < 0.01), suggesting that it may be involved in the formation of certain organs during early development. Association analysis identified three shared single nucleotide polymorphisms (SNPs), c.1073A > G, c.1139C > T and c.1154A > G in exons of ScLAP3 gene from 177 individuals of two groups, one selective strain and one wild population, which had significant effects on growth traits of S. constricta. The results provided candidate genetic markers to assist selective breeding of razor clams toward improved growth.

17.
Plant Biotechnol J ; 2019 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-31820843

RESUMO

Glucosinolates (GSLs), whose degradation products have been shown to be increasingly important for human health and plant defence, compose important secondary metabolites found in the order Brassicales. It is highly desired to enhance pest and disease resistance by increasing the leaf GSL content while keeping the content low in seeds of Brassica napus, one of the most important oil crops worldwide. Little is known about the regulation of GSL accumulation in the leaves. We quantified the levels of 9 different GSLs and 15 related traits in the leaves of 366 accessions and found that the seed and leaf GSL content were highly correlated (r = 0.79). A total of 78 loci were associated with GSL traits, and five common and eleven tissue-specific associated loci were related to total leaf and seed GSL content. Thirty-six candidate genes were inferred to be involved in GSL biosynthesis. The candidate gene BnaA03g40190D (BnaA3.MYB28) was validated by DNA polymorphisms and gene expression analysis. This gene was responsible for high leaf/low seed GSL content and could explain 30.62% of the total leaf GSL variation in the low seed GSL panel and was not fixed during double-low rapeseed breeding. Our results provide new insights into the genetic basis of GSL variation in leaves and seeds and may facilitate the metabolic engineering of GSLs and the breeding of high leaf/low seed GSL content in B. napus.

18.
Org Lett ; 21(24): 9945-9949, 2019 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-31793306

RESUMO

A gold(I) complex is reported. Interestingly, crystals of the gold(I) complex exhibit an excellent excitation wavelength-dependent emission effect at room temperature. Notably, a nearly pure white emission with Commission Internationale de L'Eclairage (CIE) 1931 chromaticity coordinates of (0.32, 0.33) is obtained upon excitation with 406 nm light.

19.
Cell Host Microbe ; 26(6): 836-843.e3, 2019 12 11.
Artigo em Inglês | MEDLINE | ID: mdl-31787524

RESUMO

African swine fever virus (ASFV) is a large double-stranded DNA virus with an icosahedral multilayered structure. ASFV causes a lethal swine hemorrhagic disease and is currently responsible for widespread damage to the pork industry in Asia. Neither vaccines nor antivirals are available and the molecular characterization of the ASFV particle is outstanding. Here, we describe the cryogenic electron microscopy (cryo-EM) structure of the icosahedral capsid of ASFV at 4.6-Å. The ASFV particle consists of 8,280 copies of the major capsid protein p72, 60 copies of the penton protein, and at least 8,340 minor capsid proteins, of which there might be 3 different types. Like other nucleocytoplasmic large DNA viruses, the minor capsid proteins form a hexagonal network below the outer capsid shell, functioning as stabilizers by "gluing" neighboring capsomers together. Our findings provide a comprehensive molecular model of the ASFV capsid architecture that will contribute to the future development of countermeasures, including vaccines.

20.
Surg Endosc ; 2019 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-31811454

RESUMO

BACKGROUND AND AIMS: Endoscopic resection (ER) is an effective and safe method for gastric submucosal tumors, mostly composed of gastrointestinal stromal tumors and leiomyomas. The role of ER in gastric schwannoma (GS) has rarely been described. Our aim was to evaluate the efficacy and safety of ER for GS. METHODS: This is a retrospective study in consecutive patients who underwent ER for GS from March 2013 to October 2018 at our center. Clinicopathological, endoscopic, and follow-up data were collected and analyzed. RESULTS: A total of 16 consecutive patients (9 females, 56.3%) were included, with a mean age of 50.4 years (range 25-75 years). The mean tumor size was 22.9 ± 15.1 mm (range 10-55 mm). Thirteen tumors (81.3%) were located in the middle third of the stomach and 12 tumors (75%) grew with intraluminal pattern. Endoscopic submucosal excavation (ESE) was performed in 7 patients while endoscopic full-thickness resection (EFTR) was done in 9 patients. R0 resection was achieved in 14 patients (87.5%). The mean operative time was 91.6 ± 52.8 min (range 36-203 min) and the mean postoperative length of hospital stays was 8.3 ± 2.7 days (range 6-13 days). No adverse events were encountered except for fevers in 2 patients. No patients required surgical resection or intervention. During long-term follow-up of mean 21.8 months (range 6-62 months), no residue, recurrence, or metastasis was observed. CONCLUSIONS: ER is effective and safe for patients with GS with favorable long-term outcomes.

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