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1.
Spectrochim Acta A Mol Biomol Spectrosc ; 227: 117675, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31670047

RESUMO

By modifying the 10-butyl-2-methoxy-10H-phenothiazine-3-carbaldehyde with malonontrile group, a new fluorescent sensor PBM for selective detection of hydrazine in ratiometric mode has been developed. Probe PBM owned the advantages of quick response (10 min), remarkable Stokes shift (168 nm for PBM, 161 nm for PBM-NH2), excellent selectivity, high sensitivity (detection limit of 63.2 nM was obtained from in vitro experiment), profound ratiometric change (82-fold) and low cytotoxicity in response to hydrazine. Additionally, it could be utilized to monitor hydrazine in gas state with various concentrations through vivid color changes and imaged hydrazine in living MCF-7 cells with excellent performance.

2.
BMJ Open ; 9(11): e028706, 2019 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-31712331

RESUMO

OBJECTIVE: To evaluate patient satisfaction and associated predictors at discharge, as well as patient experience at 30-day follow-up, in a neurosurgical enhanced recovery after surgery (ERAS) programme. DESIGN: A single-centre, prospective, randomised controlled study. SETTING: A tertiary hospital in China. PARTICIPANTS: A total of 140 neurosurgical patients aged 18-65 years old who had a single intracranial lesion and were admitted for elective craniotomy between October 2016 and July 2017 were included. INTERVENTIONS: Patients were randomised into two groups: 70 patients received care according to a novel neurosurgical ERAS protocol (ERAS group) and 70 patients received conventional perioperative care (control group). OUTCOME MEASURES: Patient satisfaction at discharge was evaluated using a multimodal questionnaire. A secondary analysis of patient experience regarding participation in the ERAS programme was conducted using a semistructured qualitative interview via telephone at 30-day follow-up. RESULTS: The mean patient satisfaction was significantly higher in the ERAS group than in the control group at discharge (92.2±4.3 vs 86.8±7.4, p=0.0001). The most important predictors of patient satisfaction included age (OR=6.934), postoperative nausea and vomiting (PONV) Visual Analogue Scale (VAS) score (OR=0.184), absorbable skin suture (OR=0.007) and postoperative length of stay (LOS) (OR=0.765). Analysis on patient experience revealed five themes: information transfer, professional support, shared responsibility and active participation, readiness for discharge, and follow-up, all of which are closely related and represent positive and negative aspects. CONCLUSIONS: Measures that include decreasing PONV VAS score, incorporating absorbable skin suture and shortening LOS seem to increase patient satisfaction in a neurosurgical ERAS programme. Analysis of data on patient experience highlights several aspects to achieve patient-centred and high-quality care. Further studies are warranted to standardise the assessment of patient satisfaction and experience in planning, employing and appraising the ERAS programme. TRIAL REGISTRATION NUMBER: ChiCTR-INR-16009662.

3.
Cancer Lett ; 469: 89-101, 2019 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-31639424

RESUMO

Salt-inducible kinase 2 (SIK2), which belongs to the AMP-activated protein kinase family, modulates various biological functions including fatty acid oxidation. However, the role of SIK2 in glucose metabolism reprogramming remains unclear in ovarian cancer (OC) cells. Here, we found that SIK2 significantly enhanced the Warburg effect of OC cells mainly through two mechanisms. On the one hand, SIK2 upregulated the expression of HIF-1α by activating the PI3K/AKT signaling pathway, which directly upregulated the transcription of major glycolytic genes to promote glycolysis. On the other hand, SIK2 promoted mitochondrial fission through phosphorylation of Drp1 at Ser616 site, which inhibited the mitochondrial oxidative phosphorylation. In addition, SIK2 promoted growth and metastasis of OC cells by promoting cell proliferation and inhibiting cell apoptosis, as well as enhancing the epithelial-mesenchymal transition. Moreover, the SIK2-mediated reprogramming of glucose metabolism played a critical role in growth and metastasis of OC cells. Collectively, our findings demonstrate that SIK2 is a crucial regulator of glucose metabolism in OC cells through activation of PI3K/AKT/HIF-1α pathway and Drp1 phosphorylation-mediated mitochondrial fission, which plays a critical oncogenic role in OC cells.

4.
Chem Asian J ; 14(21): 3791-3802, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31568646

RESUMO

Electroluminochromism (ELC) refers to an interesting phenomenon exhibited by a material whose luminescent properties can be reversibly modulated under an electrical stimulus. Such a luminescence-switching property has been widely used in various organic optoelectronic devices because it can simultaneously detect electrical and optical signals. Metal complexes are the promising candidates for ELC materials due to their sensitivity to an electrical stimulus. Herein, recent progress on electroluminochromic materials and devices based on various metal complexes has been summarized. Meanwhile, the applications of these complexes in data recording and security protection have also been discussed. Finally, a brief conclusion and outlook are presented, pointing out that the development of electroluminochromic metal complexes with excellent performance is important because they play a vital role in future intelligent optoelectronic devices.

5.
Bioresour Technol ; 294: 122236, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31610499

RESUMO

A 10-liter single chamber vertical baffle flow biocathode microbial electrochemical system (MES) with microbial separator was designed for wastewater treatment. The anode and cathode compartments were incompletely isolated by the microbial separator, which enabled module integration and centralized sludge collection of MES. The effluent COD was <50 mg L-1 with COD removal of 86 ±â€¯2% and low sludge yield rate of 0.05 ±â€¯0.02 g-sludge g-1 -COD. The MES performance was mainly restricted by biocathodes and supporting matrixes with higher permeability resulted in better cathode performance. The MES obtained the maximum power density of 67.5 ±â€¯7.8 mW m-2 with two layers of filter cloth and one layer of polyurethane sponge (S2P1) and supporting matrix with moderate permeability was more suitable in overall power generation and anode stability. The influences on bio-community of both cathodes and separators by the permeability of supporting matrixes were observed.


Assuntos
Fontes de Energia Bioelétrica , Eletricidade , Eletrodos , Esgotos , Têxteis , Águas Residuárias
6.
ACS Appl Mater Interfaces ; 11(42): 39171-39178, 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31559815

RESUMO

In the present work, high-thickness zwitterionic polymer brushes based on imidazolium salts were successfully grafted via a novel subsurface-initiated ring-opening metathesis polymerization (subsurface-initiated ROMP) from polydimethylsiloxane (PDMS), and their antifouling performance was evaluated in detail. First, an initiator-embedded PDMS was prepared via copolymerization of PDMS prepolymer and ROMP initiator, and then zwitterionic polymer brushes were grafted via subsurface-initiated ROMP from surface to subsurface of the PDMS due to the implanted ROMP initiator. Results from a series of characterization methods such as infrared spectroscopy, X-ray photoelectron spectroscopy, contact angle, and atomic force microscopy proved the zwitterionic polymer brushes' successful grafting. The grafting thickness of zwitterionic polymer brushes via subsurface-initiated ROMP can reach the micron scale, and the as-prepared zwitterionic polymer based surfaces showed good lubricating properties compared to traditional surface-initiated ROMP, which hints that polymer brushes can be grafted not only on the surface but also on the subsurface of PDMS. The protein adhesion test and biofouling assay of zwitterionic polymer brushes were tested in the laboratory, and the results indicated that the zwitterionic polymer-functionalized PDMS can effectively resist the adhesion of bovine serum albumin and algae (Porphyridium and Dunaliella) and has good anti-bacterial activity against both Escherichia coli and Staphylococcus aureus.

7.
Gynecol Oncol ; 154(3): 583-589, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31307665

RESUMO

OBJECTIVE: To evaluate the health-related quality of life (HRQOL) in locally advanced cervical cancer (LACC) patients treated with neoadjuvant concurrent chemoradiation (CCRT) or radiation (RT) alone followed by radical surgery (RS). METHODS: In a single-center retrospective study from a prospective database, 275 FIGO Stage IB2-IIIB patients who underwent CCRT/RT + RS were included. HRQOL was prospectively assessed by EORTC QLQ-C30 and EORTC QLQ-CX24 prior to any treatment (baseline) and 6 months after surgery, respectively. RESULTS: A statistically significant and clinically relevant improvement in physical functioning (P < 0.001) and role functioning (P = 0.002, P = 0.031) was observed in patients receiving either CCRT+RS or RT + RS at follow-up. In addition, quality of life (QoL), physical functioning, and social functioning were better in the RT + RS group than the CCRT+RS group after treatment (P = 0.028, P = 0.010, P = 0.014). Symptom scores of fatigue decreased in both groups over time (P < 0.001, P = 0.004) while insomnia decreased only in the RT + RS group (P = 0.042). Worsened menopausal symptoms were documented in both groups at follow-up (P = 0.001, P = 0.047), while lymphedema was deteriorated only in patients receiving CCRT + RS (P < 0.001). Sexuality scores did not differ between groups or over time with the exception of sexual worry, which was deteriorated in patients receiving RT + RS (P = 0.042). CONCLUSIONS: QLQ-C30 functioning and tumor-related symptoms scores improved while lymphedema and menopausal symptoms worsened 6 months after neoadjuvant CCRT or RT alone followed by RS in LACC patients. Patients treated with RT + RS had a generally better HRQOL compared with those receiving CCRT+RS, though further validation with prospective randomized clinical trials is warranted.


Assuntos
Neoplasias do Colo do Útero/terapia , Adulto , Idoso , Quimiorradioterapia , Quimiorradioterapia Adjuvante , Bases de Dados Factuais , Feminino , Humanos , Pessoa de Meia-Idade , Terapia Neoadjuvante , Qualidade de Vida , Radioterapia Adjuvante , Estudos Retrospectivos , Neoplasias do Colo do Útero/fisiopatologia , Neoplasias do Colo do Útero/cirurgia
8.
Adv Healthc Mater ; 8(15): e1900414, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31168955

RESUMO

Recent studies indicate that the synergistic phototherapy (SPT) process can simultaneously generate heat for photothermal therapy (PTT) and singlet oxygen (1 O2 ) for photodynamic therapy (PDT) to overcome the recurrence of tumors. However, the hypoxic environment in tumors seriously limits the therapeutic effect of the oxygen-dependent PDT, leading to the domination of PTT in the SPT process. Therefore, it is urgent to develop a novel SPT platform for overcoming hypoxia in tumors and improving the therapeutic effect of both PTT and PDT. In this work, a novel phototherapeutic platform based on a nanocomposite of aza-BODIPY/manganese dioxide (MnO2 ) is developed via simple electrostatic self-assembly. In this design, MnO2 nanosheets, which could produce heat and catalyze endogenous hydrogen peroxide (H2 O2 ) to generate oxygen, are prepared as a nanocarrier. After being coated with the as-prepared water-soluble aza-BODIPY-based polymer (PPAIB), the obtained MnO2 @PPAIB performs as a smart phototherapeutic agent for enhancing the efficiency of both PTT and PDT. More importantly, compared to PPAIB, MnO2 @PPAIB generates more heat and reactive oxygen species to realize the enhanced therapy effects of PTT and PDT. Hence, this work provides a new method to enhance the therapeutic efficacy of SPT by using a polymer/MnO2 nanoplatform to improve the oxygen concentration and produce more heat.

9.
ChemMedChem ; 14(15): 1378-1383, 2019 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-31210412

RESUMO

The development of efficient phototherapeutic agents (PTA) through rational and specific principles exhibits great potential to the biomedical field. In this study, a facile and rational strategy was used to design PTA through perturbation theory. According to the theory, both the intersystem crossing rate for singlet oxygen generation and nonradiative transition for photothermal conversion efficiency can be simultaneously enhanced by the rational optimization of donor-acceptor groups, heavy atom number, and their functional positions, which can effectively decrease the energy gap between the singlet and triplet states and increase the spin-orbit coupling constant. Finally, efficient PTA were obtained that showed excellent performance in multimode-imaging-guided synergetic photodynamic/photothermal therapy. This study therefore expands the intrinsic mechanism of organic PTA and should help guide the rational design of future organic PTA via perturbation theory.

10.
Small ; 15(18): e1901351, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30957989

RESUMO

2D metal-porphyrin frameworks (MPFs) are attractive for advanced energy storage devices. However, the inferior conductivity and low structural stability of MPFs seriously limit their application as flexible free-standing electrodes with high performance. Here, for the first time, an interlayer hydrogen-bonded MXene/MPFs film is proposed to overcome these disadvantages by intercalation of highly conductive MXene nanosheets into MPFs nanosheets via a vacuum-assisted filtration technology. The alternant insertion of MXene and MPFs affords 3D interconnected "MPFs-to-MXene-to-MPFs" conductive networks to accelerate the ionic/electronic transport rates. Meanwhile, the interlayer hydrogen bonds (F···HO and O···HO) contribute a high chemical stability due to a favorable tolerance to volume change caused by phase separation and structural collapse during the charge/discharge process. The synergistic effect makes MXene/MPFs film deliver a capacitance of 326.1 F g-1 at 0.1 A g-1 , 1.64 F cm-2 at 1 mA cm-2 , 694.2 F cm-3 at 1 mA cm-3 and a durability of about 30 000 cycles. The flexible symmetric supercapacitor shows an areal capacitance of 408 mF cm-2 , areal energy density of 20.4 µW h cm-2 , and capacitance retention of 95.9% after 7000 cycles. This work paves an avenue for the further exploration of 2D MOFs in flexible energy storage devices.

11.
Dalton Trans ; 48(26): 9631-9638, 2019 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-30869090

RESUMO

Two-dimensional (2D) metal porphyrin frameworks (MPFs) with a π-conjugated skeleton, high surface area, and more accessible active sites have been applied as active materials for supercapacitors, but the poor electrical conductivity limits the enhancement of electrochemical performance. Moreover, MPF-based flexible supercapacitors have been rarely reported. Here, for the first time, we fabricated wrinkled 2D ultrathin 5,10,15,20-tetrakis(4-carboxylphenyl)porphyrin copper(ii) (Cu-TCPP) nanosheets through the surfactant-assisted synthetic method. The as-obtained wrinkled Cu-TCPP nanosheets were further composited with conductive polypyrrole (PPy) to construct a flexible Cu-TCPP/PPy film through the combination of the electrophoretic deposition method and electrochemical polymerization technology. The wrinkled 2D ultrathin Cu-TCPP nanosheets not only offer macroporous channels for fast ion/electron transport, but also effectively reduce the transmission path of electrolyte ions. The PPy affords 3D conductive networks for rapid electron transport and enhances the electrochemical kinetics. The synergistic effect of Cu-TCPP and PPy leads to the enhanced supercapacitive performance of Cu-TCPP/PPy in comparison with the individual components. As a result, the freestanding Cu-TCPP/PPy electrode shows a capacitance of 340.6 mF cm-2 at 1 mA cm-2, 71.14 F cm-3 at 0.1 A cm-3, and 163.34 F g-1 at 0.2 A g-1, more than 45% greater than that of the pristine PPy film of 224.6 mF cm-2, 48.15 F cm-3 and 92.2 F g-1 at the corresponding current densities. Moreover, the fabricated binder-free symmetric supercapacitor possesses a maximum energy density of 2.27 µW h cm-2 at the highest power density of 50 µW cm-2. This work provides a new strategy to design 2D metal-organic framework-based flexible all-solid-state supercapacitors with high energy storage performances.

12.
Artigo em Inglês | MEDLINE | ID: mdl-30508480

RESUMO

Phototherapy, as an important class of noninvasive tumor treatment methods, has attracted extensive research interest. Although a large amount of the near-infrared (NIR) phototherapeutic agents have been reported, the low efficiency, complicated structures, tedious synthetic procedures, and poor photostability limit their practical applications. To solve these problems, herein, a donor-acceptor-donor (D-A-D) type organic phototherapeutic agent (B-3) based on NIR aza-boron-dipyrromethene (aza-BODIPY) dye has been constructed, which shows the enhanced photothermal conversion efficiency and high singlet oxygen generation ability by simultaneously utilizing intramolecular photoinduced electron transfer (IPET) mechanism and heavy atom effects. After facile encapsulation of B-3 by amphiphilic DSPE-mPEG5000 and F108, the formed nanoparticles (B-3 NPs) exhibit the excellent photothermal stabilities and reactive oxygen and nitrogen species (RONS) resistance compared with indocyanine green (ICG) proved for theranostic application. Noteworthily, the B-3 NPs can remain outstanding photothermal conversion efficiency (η = 43.0%) as well as continuous singlet oxygen generation ability upon irradiation under a single-wavelength light. Importantly, B-3 NPs can effectively eliminate the tumors with no recurrence via synergistic photothermal/photodynamic therapy under mild condition. The exploration elaborates the photothermal conversion mechanism of small organic compounds and provides a guidance to develop excellent multifunctional NIR phototherapeutic agents for the promising clinical applications.

13.
Clin Nutr ; 2018 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-30497695

RESUMO

OBJECT: The aim of this study was to evaluate the effect of preoperative oral carbohydrate loading versus fasting on the outcomes of patients undergoing elective craniotomy. METHODS: In a single-center randomized controlled study, 120 neurosurgical patients who were admitted for elective craniotomy were included and randomized into 2 groups: 58 patients received 400 mL of oral carbohydrate loading 2 h before surgery (intervention group), and 62 patients were fasting for 8 h prior to surgery as routine management (control group). The primary end point was glucose homeostasis. Secondary outcomes included handgrip strength, pulmonary function and postoperative complications. RESULTS: Better glucose homeostasis (5.6 ± 1.0 mmol/L vs. 6.3 ± 1.2 mmol/L, P = 0.001) was achieved in patients who received preoperative oral carbohydrate loading compared to fasting. Furthermore, patients in the intervention group had better handgrip strength (25.3 ± 7.1 kg vs. 19.9 ± 7.5 kg, P < 0.0001) and pulmonary function (in terms of peak expiratory flow rate) (315.8 ± 91.5 L/min vs. 270.0 ± 102.7 L/min, P = 0.036) compared to the controls postoperatively. The rates of postoperative surgical and non-surgical complications did not differ between the groups. Both postoperative and total hospital length of stay (LOS) reduced significantly in the intervention group (-3d, P < 0.0001 and P = 0.004). CONCLUSIONS: Oral carbohydrate loading given 2 h before surgery in patients undergoing elective craniotomy seems to improve glucose homeostasis, handgrip strength and pulmonary function as well as decrease LOS without increasing the risk of postoperative complications. Routine use of preoperative oral carbohydrate loading could be suggested in clinical settings, though further evaluation of its safety and efficacy is warranted.

14.
Chem Sci ; 9(36): 7236-7240, 2018 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-30288243

RESUMO

Many luminescent probes have been developed for intracellular imaging and sensing. During cellular luminescence sensing, it is difficult to distinguish species generated inside cells from those internalized from extracellular environments since they are chemically the same and lead to the same luminescence response of the probes. Considering that endogenous species usually give more information about the physiological and pathological parameters of the cells while internalized species often reflect the extracellular environmental conditions, we herein reported a series of cyclometalated iridium(iii) complexes as phosphorescent probes that are partially retained in the cell membrane during their cellular uptake. The utilization of the probes for sensing and distinguishing between exogenous and endogenous analytes has been demonstrated using hypoxia and hypochlorite as two examples of target analytes. The endogenous analytes lead to the luminescence response of the intracellular probes while the exogenous analytes are reported by the probes retained in the cell membrane during their internalization.

15.
Chembiochem ; 2018 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-30267457

RESUMO

It is a huge challenge to avoid irreversible damage to normal tissues during irradiation in photodynamic therapy (PDT) for cancer. An effective strategy is to develop smart photosensitizers, which exhibit amplified generation of reactive oxygen species (ROS) through triggering specific reaction in the tumor microenvironment. In this work, we designed a class of glutathione (GSH)-activatable photosensitizers (Ir1 and Ir4) based on an effective strategy of GSH-induced nucleophilic substitution reaction. The addition of GSH, induced changes in both phosphorescence intensity and lifetime of photosensitizers with high sensitivity. Importantly, the amount of singlet oxygen generated was increased significantly by GSH-induced activation reaction. Hence, the photosensitizers can selectively distinguish cancer cells from normal cells through luminescence and lifetime imaging, and can amplify PDT effects in cancer cells, owing to the evidently higher level of GSH compared to normal cells. This work presents a novel paradigm for GSH-amplified PDT against cancer cells and provides a new avenue for smart-responsive theranostic systems that can avoid nonspecific damage to normal cells.

16.
ACS Appl Mater Interfaces ; 10(42): 35838-35846, 2018 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-30260621

RESUMO

Reactive oxygen species (ROS), when beyond the threshold, can exhaust the capacity of cellular antioxidants and ultimately trigger cell apoptosis in tumor biology. However, the roles of hypochlorite (ClO-) in this process are much less clear compared with those of ROS, and its detection is easily obstructed by tissue penetration and endogenous fluorophores. Herein, we first synthesized a near-infrared (NIR) ratiometric ClO- probe (Ir NP) composed of two kinds of phosphorescent iridium(III) complexes (Ir1 and Ir2) encapsulated with amphiphilic DSPE-mPEG5000. Ir NPs are dual-emissive and show obvious changes in phosphorescence intensity ratios and lifetimes of two emission bands upon exposure to ClO-. During the ClO- detection, ratiometric photoluminescence imaging is much more reliable over the intensity-based one for its self-calibration, while time-resolved photoluminescence imaging (TRPI) could distinguish the phosphorescence with long lifetime of Ir NPs from short-lived autofluorescence of tissues, resulting in the high accuracy of ClO- determination. With NIR emission, a long phosphorescence lifetime, fast response, and excellent biocompatibility, Ir NPs were applied to the detection of ClO- in vitro and in vivo by means of ratiometric phosphorescence imaging and TRPI with high signal-to noise-ratios (SNR). Importantly, we demonstrated the elevated ClO- in elesclomol-stimulated tumors in living mice for the first time, which holds great potential for the visualization of the boost of ClO- in anti-carcinogen-treated tumors and the further investigation of ROS-related oncotherapeutics.


Assuntos
Hidrazinas/uso terapêutico , Ácido Hipocloroso/química , Raios Infravermelhos , Luminescência , Nanopartículas/química , Neoplasias/tratamento farmacológico , Animais , Linhagem Celular Tumoral , Sobrevivência Celular , Células HeLa , Humanos , Hidrazinas/farmacologia , Irídio/química , Camundongos , Nanopartículas/ultraestrutura , Neoplasias/patologia
17.
ACS Appl Mater Interfaces ; 10(37): 31008-31018, 2018 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-30130088

RESUMO

A scalable and low-cost strategy is developed to fabricate a novel CuS/SiO2-based nanotherapeutic agent for dual-model imaging-guided photothermal/photodynamic combined therapy. In this design, mesoporous silica nanoparticles (MSNs) with CuS bundled in the channel are obtained in aqueous solution via in situ growth route for the first time. Furthermore, to achieve a more efficient therapy, photosensitizer (complex Ir-2) and bovine serum albumin are sequentially assembled via layer-by-layer method. The as-prepared complex Ir-2 presents a remarkably high 1O2 generation (ΦΔ = 1.3) under light illumination to offer effective photodynamic cell killing, and MSN/CuS exhibits high photothermal conversion efficiency (η = 31.7%) under illumination by 808 nm light to offer hyperthermia tumor ablation. In vitro and in vivo analyses show that the as-obtained nanotherapeutic agents exhibit excellent performance in tumor therapy even under irradiation with low power because of the high yield of 1O2 combined with the high photothermal conversion efficiency. Additionally, the nanotherapeutic agents are readily visualized in vivo via near-infrared fluorescence and thermal imaging. More importantly, based on the strategy of in situ growth and layer-by-layer assembly developed in this study, the development of other "all-in-one" multifunctional theranostic platform with high efficiency can be predictable.

18.
Adv Healthc Mater ; 7(18): e1800606, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30047582

RESUMO

It is always a huge challenge to develop novel near-infrared (NIR) phototherapeutic agents suitable for imaging-guided cancer therapy. In order to clarify the positive heavy atom effects on the photodynamic and photothermal efficiencies of phototherapeutic agents, a series of chlorine-, bromide-, or iodine-substituted aza-BODIPYs (B2, B3, and B4, respectively) are designed and synthesized. Among them, B4 exhibits both excellent photodynamic and photothermal effects (singlet oxygen yield of B4 is 1.57 times more than that of B3) and excellent photothermal effects (1.3 °C higher than that of B3). Then, nanoparticles of B4 (IABNs) with excellent biocompatibility are prepared by coating hydrophobic B4 with hydrophilic polymer DSPE-mPEG5000 . IABN exhibits high photostability, excellent biocompatibility, and low dark toxicity both in vivo and in vitro. Furthermore, IABN shows the enhanced photodynamic effect and high photothermal conversion efficiency (34.8%). In addition, the strong fluorescence emission of IABN makes it suitable for fluorescence imaging-guided tumor therapy in vivo. Finally, IABN has successfully healed the Hela tumor-bearing mice under NIR fluorescence imaging- and photothermal imaging-guided synergistic photothermal and photodynamic therapy with low side effects, demonstrating that it is promising for future clinical applications.

19.
Adv Healthc Mater ; 7(16): e1800309, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29968378

RESUMO

Peroxynitrite (ONOO- ), a potent biological oxidant, which has a short half-life in physiological conditions, is related to many diseases. Accurate peroxynitrite determination with superior selectivity and sensitivity is important for understanding biological roles of peroxynitrite in different health and disease tissues. Autofluorescence is an inevitable interference in luminescence biodetection and bioimaging, which often reduces signal-to-noise ratio during detection. In this work, a phosphorescent peroxynitrite nanoprobe (MSN-ONOO) which displays two emission bands is prepared by immobilizing two long-lived phosphorescent iridium(III) complexes that are peroxynitrite-activable and -inert, respectively, into water-dispersible mesoporous silica nanoparticles. Owing to the fast response rate, excellent sensitivity and outstanding selectivity of the nanoprobe toward peroxynitrite, it is further used for peroxynitrite determination in vitro and in vivo via ratiometric photoluminescence imaging. More notably, taking advantage of the long-lived phosphorescence of MSN-ONOO, in vivo elevated peroxynitrite is imaged with diminished autofluorescence interference and improved signal-to-noise ratio via time-resolved photoluminescence imaging. As far as it is known, this is the first time for endogenous peroxynitrite detection in vivo via the time-resolved photoluminescence imaging. Furthermore, the production of peroxynitrite in inflamed tissues is visualized.

20.
J Am Chem Soc ; 140(25): 7827-7834, 2018 Jun 27.
Artigo em Inglês | MEDLINE | ID: mdl-29874455

RESUMO

Hypoxia and hyperoxia, referring to states of biological tissues in which oxygen supply is in sufficient and excessive, respectively, are often pathological conditions. Many luminescent oxygen probes have been developed for imaging intracellular and in vivo hypoxia, but their sensitivity toward hyperoxia becomes very low. Here we report a series of iridium(III) complexes in which limited internal conversion between two excited states results in dual phosphorescence from two different excited states upon excitation at a single wavelength. Structural manipulation of the complexes allows rational tuning of the dual-phosphorescence properties and the spectral profile response of the complexes toward oxygen. By manipulating the efficiency of internal conversion between the two emissive states, we obtained a complex exhibiting naked-eye distinguishable green, orange, and red emission in aqueous buffer solution under an atmosphere of N2, air, and O2, respectively. This complex is used for intracellular and in vivo oxygen sensing not only in the hypoxic region but also in normoxic and hyperoxic intervals. To the best of our knowledge, this is the first example of using a molecular probe for simultaneous bioimaging of hypoxia and hyperoxia.

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