Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 78
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Artigo em Inglês | MEDLINE | ID: mdl-32143863

RESUMO

PURPOSE: The aim of this study was to evaluate the feasibility and diagnostic accuracy of core needle biopsy (CNB) in patients with focal fluorodeoxyglucose (FDG) uptake on positron emission tomography/computed tomography (PET/CT) in deep regions of the head and neck, with the guidance of infrared navigation integrated with PET. MATERIALS AND METHODS: Patients with suspected primary or recurrent malignancies of the head and neck on PET/CT, from June 2016 to December 2018, were included. Before CNB, the region of interest was delineated and the ideal needle entry points, target sites, and a number of trajectories were designed on iPlan CMF 3.0. CNB was performed with the guidance of infrared navigation integrated with PET, according to the pre-plan. Sensitivity and diagnostic accuracy were analyzed by comparing the biopsy results with the final diagnosis. RESULTS: Thirty-one consecutive patients were included. Among the 31 lesions, 18 were skull base, six were infratemporal fossa, and seven were maxillary region. The median values for SUVmax, SUVmean, and MTV were 6.09 (range: 1.43-24.67), 3.41 (range: 0.38-20.96), and 25.83 (range: 3.54-361.94) for the 31 lesions, respectively. Combined needle approaches were employed, including temporal (nine), subzygomatic (19), paramaxillary (11), and retromandibular (16) approaches. The depths of the 31 deep-region lesions, measured from the needle entry site on the skin to the target point, ranged from 1.33 cm to 7.82 cm (median 4.25 cm). There were three non-diagnostic lesions resulting from CNB, and these were all skull base. The diagnostic accuracy was 90.3%, while the sensitivity was 88%. According to the binary logistic regression for the final diagnosis, the only significant parameter was SUVmax. CONCLUSION: With the guidance of navigation integrated with PET, CNB is a feasible and accurate diagnostic modality, which is also an alternative to open biopsy in patients with suspected primary or recurrent malignancies in deep regions of the head and neck on PET/CT.

2.
Nanotechnology ; 2020 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-32155614

RESUMO

The shuttling-effect of soluble lithium-based polysulfides (LiPS) represents one of the main obstacles for the practical application of lithium-sulfur (Li-S) battery. Herein, to address this issue, a flexible Li-S interlayer consisting of two-dimensional α-Co(OH)2 nano-plate and graphene oxide (GO) is developed using a simple vacuum filtration technique. In the interlayer, the α-Co(OH)2 and GO are assembled into a layered structure forming a physical barrier for the shuttling of LiPS. Additionally, the α-Co(OH)2 offers strong chemical adsorption and efficient catalysis performance towards LiPS conversion, further inhibiting its shuttling. Attributed to these beneficial features of the interlayer, the Li-S battery delivers an initial discharge capacity of 834 mAh g-1 at the current density of 1 C. More importantly, after 300 cycles, a high discharge capacity of 590 mAh g-1 was retained, corresponding to a low capacity fading rate of 0.1 % per cycle. This work might be of great interest for the feasible and scalable preparation of multifunctional interlayer in Li-S battery.

3.
Artigo em Inglês | MEDLINE | ID: mdl-32173779

RESUMO

Accumulation of heavy metals in agricultural soils due to human production activities-mining, fossil fuel combustion, and application of chemical fertilizers/pesticides-results in severe environmental pollution. As the transmission of heavy metals through the food chain and their accumulation pose a serious risk to human health and safety, there has been increasing attention in the investigation of heavy metal pollution and search for effective soil remediation technologies. Here, we summarized and discussed the basic principles, strengths and weaknesses, and limitations of common standalone approaches such as those based on physics, chemistry, and biology, emphasizing their incompatibility with large-scale applications. Moreover, we explained the effects, advantages, and disadvantages of the combinations of common single repair approaches. We highlighted the latest research advances and prospects in phytoremediation-chemical, phytoremediation-microbe, and phytoremediation-genetic engineering combined with remediation approaches by changing metal availability, improving plant tolerance, promoting plant growth, improving phytoextraction and phytostabilization, etc. We then explained the improved safety and applicability of phytoremediation combined with other repair approaches compared to common standalone approaches. Finally, we established a prospective research direction of phytoremediation combined with multi-technology repair strategy.

4.
J Environ Sci (China) ; 89: 218-226, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31892393

RESUMO

The current study analyzes the contribution of 10 water quality parameters (including pH, turbidity, conductivity, total dissolved solids (TDS), hardness, total organic carbon (TOC), alkalinity, calcium ions, chlorides and sulfates) to corrosion extent of stainless steel valves taken from different locations in a reverse osmosis system of a reclaimed water plant. The valves were in service for 5 years. Raman spectroscopy and X-ray photoelectron spectroscopy analyses are conducted to quantify corrosion products on different valves under various water quality conditions. On that basis, bivariate and multivariate regression analyses between the 10 water quality parameters and the corrosion extent of valve specimens (represented by metal loss percentage (MLP) values) are carried out to check the contribution of those water quality parameters to MLP. The results indicate that the proportions of metal oxides as corrosion products vary according to the corrosion extent of the valves. Although no linear correlation is found, all 10 water quality parameters except for pH show a significant positive correlation with the MLP values of the valve specimens. Moreover, results of multivariate regression suggest that the variation of MLP can be explained by turbidity, TDS, TOC and sulfates. A positive contribution of turbidity, TDS and TOC to MLP is observed, whereas the contribution of sulfates is negative. The results from the current work help to identify the reasons for water quality-induced failure of stainless steel equipment in RO systems.

5.
J Oral Maxillofac Surg ; 78(1): 167-172, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31604061

RESUMO

PURPOSE: The aim was to evaluate the safety and efficacy of subcutaneous injection of hyaluronic acid in decreasing acute skin toxicity after adjuvant interstitial brachytherapy in parotid gland cancer patients. MATERIALS AND METHODS: Patients with histologically proven parotid gland cancer who would be treated with adjuvant interstitial brachytherapy were included in this nonrandomized controlled trial. Participants were nonrandomly divided into the experimental group and control group. Participants in the experimental group received an injection of hyaluronic acid subcutaneously immediately after interstitial brachytherapy during the operation. Acute toxicity was evaluated in the first 2 months. RESULTS: Thirty consecutive participants were included from April to September 2018. Twenty participants were in the experimental group, and 10 were in the control group. The median volume of hyaluronic acid was 8 mL (range, 4 to 11 mL). In total, the incidence of acute skin toxicity was 40% (8 of 20 patients) and 100% (10 of 10 patients) in the experimental group and control group, respectively. The difference in the dose delivered to 90% of the target volume of the affected skin was significant between the pre-plan (mean, 36.93 Gy) and the actuarial quality verification (mean, 27.70 Gy) in the experimental group (P = .004). The difference in scoring of acute skin toxicity was significant between the experimental and control groups (P = .001). No clear correlation was found between the dose delivered to 90% of the target volume of the affected skin and the scoring of acute skin toxicity (P = .266). CONCLUSIONS: Subcutaneous injection of hyaluronic acid was safe and efficient in decreasing acute skin toxicity after adjuvant interstitial brachytherapy in parotid gland cancer patients according to the preliminary results.

6.
EJNMMI Res ; 9(1): 89, 2019 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-31511990

RESUMO

BACKGROUND: The aim of this study was to evaluate the prognostic value of positron emission tomography (PET) parameters and the PET texture features of fluorine 18-fluorodeoxyglucose ([18F]FDG) uptake on pretreatment PET/computed tomography (CT) in patients with locally advanced salivary gland carcinoma treated with interstitial brachytherapy. METHODS: Forty-three patients with locally advanced salivary gland carcinoma of the head and neck were treated with 125I interstitial brachytherapy as the sole modality and underwent [18F]FDG PET/CT scanning before treatment. Tumor segmentation and texture analysis were performed using the 3D slicer software. In total, 54 features were extracted and categorized as first-order statistics, morphology and shape, gray-level co-occurrence matrix, and gray-level run length matrix. Up to November 2018, the follow-up time ranged from 6 to 120 months (median 18 months). Cumulative survival was calculated by the Kaplan-Meier method. Factors between groups were compared by the log-rank test. Multivariate Cox regression analysis with a backward conditional method was used to predict progression-free survival (PFS). RESULTS: The 3- and 5-year locoregional control (LC) rates were 55.4% and 37.0%, respectively. The 3- and 5-year PFS rates were 51.2% and 34.1%, respectively. The 3- and 5-year overall survival (OS) rates were 77.0% and 77.0%, respectively. Univariate analysis revealed that minimum intensity, mean intensity, median intensity, root mean square, and long run emphasis (LRE) were significant predictors of PFS, whereas clinicopathological factors, conventional PET parameters, and PET texture features failed to show significance. Multivariate Cox regression analysis showed that minimum intensity and LRE were significant predictors of PFS. CONCLUSIONS: The texture analysis of pretreatment [18F]FDG PET/CT provided more information than conventional PET parameters for predicting patient prognosis of locally advanced salivary gland carcinoma treated with interstitial brachytherapy. The minimum intensity was a risk factor for PFS, and LRE was a favorable factor in prognostic prediction according to the primary results.

7.
Water Res ; 164: 114888, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31377525

RESUMO

Stringent regulations and deteriorating source water quality could greatly influence the water production capacity of drinking water treatment plants (DWTPs). Using models to predict the performance of DWTPs under stress provides valuable information for decision making and future planning. A hybrid statistic model named HANN was established by combining artificial neural network (ANN) with genetic algorithm (GA) aiming at forecasting the overall performance of DWTPs nationwide in China. Monthly data from 45 DWTPs across China was employed. Water quality parameters like temperature and chemical oxygen demand (COD) and operational parameters like electricity consumption and chemical consumption were selected as input variables, while drinking water production was employed as the output. Both preliminary data analysis and principal component analysis (PCA) suggested a clear non-linear relationship between the input and output variables. The structure of the HANN model was optimized by employing the lowest mean squared error (MSE) as the indicator. The resultant HANN model performed well when simulating the training datasets. Its predictive accuracy for the independent test datasets was enhanced when feeding more training datasets and the performance was constantly higher than the independent multi-layered ANN models using the coefficient of determination (R2) as the indicator, indicating the HANN model was capable of capturing complex non-linear relationship and extrapolation. Results from Accuracy test, Garson sensitivity analysis and Analysis of Variance (ANOVA) suggested the quantity of water produced by DWTPs was closely linked to water quality and operational parameters. The scenario analysis showed that the HANN model was capable of predicting water production variation based on the parameter variations, indicating that the HANN model could be a general management tool for decision makers and DWTP managers to make plans in advance of regulatory changes, source water quality variations and market demand.


Assuntos
Água Potável , Purificação da Água , Algoritmos , China , Qualidade da Água
8.
Biotechnol Lett ; 41(8-9): 1023-1031, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31203568

RESUMO

OBJECTIVE: The present study evaluated the antibacterial activity and mode of action of fibrauretine on Escherichia coli (E. coli) and Staphylococcus aureus, and synergistic effect with kanamycin against multi-drug resistant E. coli. RESULTS: The fibrauretine exhibited inhibitory effect on the growth of the tested bacteria with minimum inhibitory concentration (MIC) and minimum bactericidal concentration of 2.5-5 and 5-10 mg/ml, respectively. Morphological changes of cell microstructure were observed after adding fibrauretine at MIC. The mode of action was further confirmed by measuring release of 260-nm absorbing materials and extracellular potassium ions. Checkerboard dilution test suggested that fibrauretine exhibited synergistic activity when combined with kanamycin (FICI ranging from 0.5625 to 0.625). CONCLUSIONS: Our results indicated that fibrauretine exerted synergistic effect with kanamycin and its antibacterial mode of action mainly attributed to disruption of cell membrane integrity.


Assuntos
Antibacterianos/farmacologia , Farmacorresistência Bacteriana Múltipla , Sinergismo Farmacológico , Escherichia coli/efeitos dos fármacos , Canamicina/farmacologia , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/farmacologia , Testes de Sensibilidade Microbiana , Viabilidade Microbiana/efeitos dos fármacos , Compostos Fitoquímicos/isolamento & purificação , Extratos Vegetais/isolamento & purificação , Staphylococcus aureus/efeitos dos fármacos
9.
Head Neck ; 41(9): 3219-3225, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31116488

RESUMO

BACKGROUND: This study aimed to determine the effectiveness and safety of surgery combined with postoperative 125 I seed brachytherapy for treatment of primary mucoepidermoid carcinoma (MEC) of the parotid gland. METHODS: Retrospective analysis of data of patients with MEC (n = 108) treated with surgery plus postoperative 125 I seed brachytherapy between 2004 and 2016. Overall survival (OS), disease-free survival (DFS), local control rate (LCR), distant metastasis, and radiation-associated toxicities were analyzed, and factors affecting outcomes were evaluated. RESULTS: The 5- and 10-year OS were 98.8% and 95.8%, respectively. The DFS and LCR at 5 and 10 years were all 91.4%. Age ≥ 60 years (hazard ratio [HR] = 6.86, 95% confidence interval [CI]: 1.54-30.55) and T4 disease (HR = 7.15, 95% CI: 1.40-36.52) were poor prognostic factors. Acute radiation-associated toxicities were minor. CONCLUSION: Surgery plus 125 I seed brachytherapy appears to be an effective treatment for parotid gland MEC, capable of providing satisfactory outcomes with few complications.

10.
Materials (Basel) ; 12(7)2019 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-30987253

RESUMO

Friction stir welding (FSW) with a Zr interlayer was employed to join dissimilar alloys of 6061 Al and AZ31 Mg. The microstructures of Al/Mg and Al/Zr/Mg joints were investigated by optical microscopy (OM), scanning electron microscopy (SEM), and energy dispersive X-ray spectrometer (EDS). The results showed that the central part of the Zr interlayer was smashed and intermixed with the base materials in the stir zone, whereas the undamaged part remained stable at the Al/Mg interface. The formation of Al-Mg intermetallic compounds (IMCs) was suppressed by the Zr interlayer due to its synergetic effects of chemical modification and thermal barrier. The electrochemical measurements revealed a differentiated corrosion behavior for each joint, where the corrosion rate of representative regions increased in the order of Al alloy < Mg alloy < heat-affected zone < stir zone. The immersion tests indicated an enhancement in corrosion resistance for the Al/Zr/Mg joint compared with the Al/Mg joint, which is owing to the mitigated galvanic corrosion between the base materials by the Zr interlayer.

11.
J Environ Manage ; 238: 201-209, 2019 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-30851559

RESUMO

Risk of cross-connection is becoming higher due to greater construction of potable-reclaimed water dual distribution systems. Cross-connection events can result in serious health concerns and reduce public confidence in reclaimed water. Thus, reliable, cost-effective and real-time online detection methods for early warning are required. The current study carried out pilot-scale experiments to simulate potable-reclaimed water pipe cross-connection events for different mixing ratios (from 30% to 1%) using machine learning methods based on multiple conventional water quality parameters. The parameters included residual chlorine, pH, turbidity, temperature, conductivity, oxidation-reduction potential and chemical oxygen demand. The results showed that correlated variation occurred among water quality parameters at the time of the cross-connection event. A single parameter-based method can be effective at high mixing ratios, but not at low mixing ratios. The direct supporting vector machine (SVM)-based method managed to overcome this drawback, but coped poorly with abnormal readings of water parameter sensors. In that respect, a Pearson correlation coefficient (PCC)-SVM-based method was developed. It provided not only high detection performance under normal conditions, but also remained reliable when abnormal readings occurred. The detection accuracy and true positive rate of this method was still over 88%, and the false positive rate was below 12%, given a sudden variation of an individual water quality parameter. The receiver operating characteristic curves further confirmed the promising practical applicability of this PCC-SVM-based method for early detection of cross-connection events.


Assuntos
Água Potável , Fumar Cachimbo de Água , Águas Residuárias , Qualidade da Água , Abastecimento de Água
12.
Chem Asian J ; 14(2): 322-327, 2019 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-30507065

RESUMO

In this study, inverse opal TiO2-x photonic crystals (IO-TiO2-x ) have been successfully synthesized by a two-step calcination. The whole synthesis is safe and feasible. Additionly, the reduction degree and the structure of IO-TiO2-x can be precisely controlled. A series of IO-TiO2-x samples with different reduction degree were prepared and characterized. The TEM images show that the obtained samples possess a 3D-ordered macroporous inverse opal structure. The reduced Ti atoms/oxygen vacancies were confirmed by Raman and XPS spectroscopy. All IO-TiO2-x samples showed better photoelectric properties than those of common TiO2 which indicates their great potential to be applied to photoelectric fields. The improvement of photoelectric properties is attributed to the efficient electron-hole separation efficiency induced by moderately reduced Ti atoms/oxygen vacancies. Meanwhile, the 3D-ordered macroporous inverse opal structure and the band gap are regulated to "capture" more solar energy. This new approach is proven to be a meaningful method to synthesize high-performance TiO2 materials.

13.
J Environ Manage ; 223: 658-667, 2018 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-29975893

RESUMO

Urbanization, one of the predominant trends of the 21st century, places great stress on urban water supply networks. This paper aimed to identify the most important variables driving urban water supply patterns in China, a region which has seen rapid urban growth in the last few decades. In addition, a principal component analysis-informed urban water sustainability index was developed in order to benchmark cities. The research involved applying statistical learning and other analytical methods to 12 years of urban water supply data for 627 cities across China. The findings were as follows: (1) PCA showed that approximately 46.8% of variability in the data could be explained by two principal components. Component 1 (37.26%) was more closely associated with variables related to water supply and sale, supply pipelines, and water supply finance. C2 (9.51%) was clearly related to urban water prices and average per capita water use. (2) Random forest and XGBoost algorithms were effective in classifying cities according to their region, with model testing accuracies of 87.69% and 88.32% respectively. (3) Chinese cities have consistently suffered water loss/leakage rates above 20% since 2001, and water prices are closely associated with leakage. (4) China's urban water sustainability has increased by just 3.56% between 2001 and 2013; Southwest China saw the highest growth rate in urban water supply sustainability. The implications of our research effort will be useful for decision makers in water-stressed urban areas around the world who are seeking novel insights in how to leverage statistical learning techniques to gain insights into urban drinking water supply patterns.


Assuntos
Água Potável , Urbanização , China , Cidades , População Urbana , Abastecimento de Água
14.
J Craniomaxillofac Surg ; 46(8): 1263-1267, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29803367

RESUMO

When combined with iliac bone, perforator flaps are more chimeric, and there is increased mobile skin island to reconstruct soft tissue defects in the oral and maxillofacial region. This study examined oromandibular defects reconstructed using deep circumflex iliac artery perforator flap with iliac crest (DCIAPF). We retrospectively reviewed records of 23 patients with mandibular defects received DCIAPFs after oncological resection for oromandibular reconstruction from November 2015 to August 2016. All perforators, identified before surgery by Doppler examination, were terminal perforators of DCIA. DCIAPFs were successfully harvested in all patients. The flap survival rate was 95.6% (22/23); one flap failed due to artery spasm. Three patients developed slight skinedge necrosis in the skin island. Anatomical reconstruction contour of the mandible and sufficient bone length and height were achieved, with no serious donor-site complications during the follow-up period. The results demonstrated that DCIAPF is a favorable single-flap option for oromandibular reconstruction after oncological resection with fewer donor-site complications because of its adequate bone tissue and satisfactory soft tissue, with a constant location of the perforator.


Assuntos
Artéria Ilíaca/cirurgia , Ílio/transplante , Reconstrução Mandibular/métodos , Retalho Perfurante/cirurgia , Adolescente , Adulto , Idoso , Criança , Feminino , Humanos , Masculino , Neoplasias Mandibulares/cirurgia , Pessoa de Meia-Idade , Estudos Retrospectivos , Adulto Jovem
15.
Sci Total Environ ; 637-638: 1466-1470, 2018 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-29801239

RESUMO

China is increasing its use of non-conventional water sources - seawater desalination, surface water transfer and wastewater reuse - to meet demand. Getting and treating water from these sources generally requires more energy than is needed for local freshwater sources. This increases the cost and greenhouse gas emissions associated with water supply. It also leads us to the question: are alternative water sources necessary and, if so, which source should be preferred? Here we argue that reclaiming and reusing wastewater is often the least energy-intensive alternative source for water-scarce areas of China, particularly when energy present in wastewater is recovered during the process.


Assuntos
Eliminação de Resíduos Líquidos/métodos , Reatores Biológicos , China , Fontes Geradoras de Energia , Água do Mar , Esgotos , Águas Residuárias , Purificação da Água , Abastecimento de Água
16.
Bioresour Technol ; 254: 290-299, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29398290

RESUMO

This review highlights the importance of conducting biological stability evaluation due to water reuse progression. Specifically, assimilable organic carbon (AOC) has been identified as a practical indicator for microbial occurrence and regrowth which ultimately influence biological stability. Newly modified AOC bioassays aimed for reclaimed water are introduced. Since elevated AOC levels are often detected after tertiary treatment, the review emphasizes that actions can be taken to either limit AOC levels prior to disinfection or conduct post-treatment (e.g. biological filtration) as a supplement to chemical oxidation based approaches (e.g. ozonation and chlorine disinfection). During subsequent distribution and storage, microbial community and possible microbial regrowth caused by complex interactions are discussed. It is suggested that microbial surveillance, AOC threshold values, real-time field applications and surrogate parameters could provide additional information. This review can be used to formulate regulatory plans and strategies, and to aid in deriving relevant control, management and operational guidance.


Assuntos
Carbono , Purificação da Água , Desinfecção , Água , Microbiologia da Água , Abastecimento de Água
17.
J Oral Maxillofac Surg ; 76(4): 886-893, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29178981

RESUMO

PURPOSE: This study evaluated the computer-aided approach to the reconstruction of mandibular defects using a vascularized iliac-crest flap. MATERIALS AND METHODS: From December 2015 to October 2016, 14 patients (8 men and 6 women) 18 to 64 years old (median age, 29 yr) were treated at the Peking University School and Stomatology Hospital (Beijing, China). Biopsy specimens from all patients were subjected to histologic examination before segmental mandibulectomy. Computer-based surgical techniques, including virtual surgical planning, computer-aided design and manufacturing, rapid prototyping, and intraoperative navigation, were used to restore the anatomic continuity and configuration of the mandible using a vascularized iliac-crest flap. Two transverse dimensions and 1 anteroposterior (A-P) dimension were evaluated based on the virtual plan and postoperative computed tomogram. Lines from condylar head to condylar head and from gonial angle to gonial angle were defined as the transverse dimensions. A perpendicular line drawn from the mandibular midline to the center point on the condylar head to condylar head measurement was defined as the A-P dimension. Complications were evaluated during follow-up. RESULTS: The flap success rate was 92.9% (13 of 14), with 1 flap failure. After the operation, there were no other serious complications in 13 of the 14 patients, who exhibited a good mandibular configuration with good occlusion. Furthermore, the height of bone graft was sufficient for implants. Healing of the recipient and donor sites with no serious complication was uneventful. The average surgical errors in the A-P dimension and transverse dimensions were 1.8 ± 1.0 mm (range, 0.2 to 3.7 mm), 2.2 ± 1.1 mm (range, 0.9 to 5.0 mm), and 2.6 ± 1.6 mm (range, 0.3 to 7.2 mm), respectively. CONCLUSIONS: The use of these digital techniques was found to be a viable option for reconstruction of mandibular defects, but the results should be interpreted cautiously because of the small number of patients and the relatively short follow-up.


Assuntos
Retalhos de Tecido Biológico/cirurgia , Ílio/transplante , Reconstrução Mandibular/métodos , Cirurgia Assistida por Computador/métodos , Adolescente , Adulto , Feminino , Humanos , Ílio/irrigação sanguínea , Imagem Tridimensional , Masculino , Pessoa de Meia-Idade , Adulto Jovem
18.
J Exp Med ; 215(1): 141-157, 2018 01 02.
Artigo em Inglês | MEDLINE | ID: mdl-29203539

RESUMO

The median overall survival for children with diffuse intrinsic pontine glioma (DIPG) is less than one year. The majority of diffuse midline gliomas, including more than 70% of DIPGs, harbor an amino acid substitution from lysine (K) to methionine (M) at position 27 of histone 3 variant 3 (H3.3). From a CD8+ T cell clone established by stimulation of HLA-A2+ CD8+ T cells with synthetic peptide encompassing the H3.3K27M mutation, complementary DNA for T cell receptor (TCR) α- and ß-chains were cloned into a retroviral vector. TCR-transduced HLA-A2+ T cells efficiently killed HLA-A2+H3.3K27M+ glioma cells in an antigen- and HLA-specific manner. Adoptive transfer of TCR-transduced T cells significantly suppressed the progression of glioma xenografts in mice. Alanine-scanning assays suggested the absence of known human proteins sharing the key amino acid residues required for recognition by the TCR, suggesting that the TCR could be safely used in patients. These data provide us with a strong basis for developing T cell-based therapy targeting this shared neoepitope.


Assuntos
Antígenos de Neoplasias/genética , Antígenos de Neoplasias/imunologia , Glioma/genética , Glioma/imunologia , Histonas/genética , Histonas/imunologia , Mutação , Linfócitos T/imunologia , Linfócitos T/metabolismo , Transferência Adotiva , Sequência de Aminoácidos , Aminoácidos , Animais , Apresentação do Antígeno , Antígenos de Neoplasias/química , Cromatografia Líquida , Modelos Animais de Doenças , Mapeamento de Epitopos , Feminino , Glioma/patologia , Glioma/terapia , Antígenos HLA-A/imunologia , Antígenos HLA-A/metabolismo , Histonas/química , Humanos , Imunoterapia Adotiva , Camundongos , Camundongos Transgênicos , Peptídeos/química , Peptídeos/imunologia , Peptídeos/metabolismo , Ligação Proteica , Receptores de Antígenos de Linfócitos T/genética , Receptores de Antígenos de Linfócitos T/metabolismo , Especificidade do Receptor de Antígeno de Linfócitos T/imunologia , Espectrometria de Massas em Tandem , Ensaios Antitumorais Modelo de Xenoenxerto
19.
J Zhejiang Univ Sci B ; 18(10): 917-920, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28990382

RESUMO

Great variations have been found in composition and content of the essential oil of Zanthoxylum bungeanum Maxim. (Rutaceae), resulting from various factors such as harvest time, drying and extraction methods (Huang et al., 2006; Shao et al., 2013), solvent and herbal parts used (Zhang, 1996; Cao and Zhang, 2010; Wang et al., 2011). However, in terms of artificial introduction and cultivation, there is little research on the chemical composition of essential oil extracted from Z. bungeanum Maxim. cultivars, which have been introduced from different origins. In this study, the composition and content of essential oil from six cultivars (I-VI) have been investigated. They were introduced and cultivated for 11 years in the same cultivation conditions. Cultivars were as followings: Qin'an (I) cultivar originally introduced from Qin'an City in Gansu Province; Dahongpao A (II) from She County in Hebei Province; Dahongpao B (III) from Fuping County; Dahongpao C (IV) from Tongchuan City; Meifengjiao (V) from Feng County; and, Shizitou (VI) from Hancheng City, in Shaanxi Province, China. This research is expected to provide a theoretical basis for further introduction, cultivation, and commercial development of Z. bungeanum Maxim.


Assuntos
Óleos Voláteis/análise , Zanthoxylum/química , Zanthoxylum/crescimento & desenvolvimento
20.
J Vet Med Sci ; 79(10): 1678-1681, 2017 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-28867688

RESUMO

Twenty-three isolates of Pasteurella multocida were tested for susceptibility to six aminoglycoside agents and screened by polymerase chain reaction for the presence of aminoglycoside resistance genes. In addition, mutations in the resistance-determining region of strains showing a high level of induced resistance to spectinomycin strains were examined. Susceptibility testing showed that all of the isolates were resistant to at least two types of aminoglycosides, and that the most effective antimicrobial was spectinomycin. The resistance genes aphA1, strB and aacA4 were present in all 23 isolates. In the three induced spectinomycin-resistant strains, a 9-bp deletion in rpsE that encodes ribosomal protein S5 was detected.


Assuntos
Aminoglicosídeos/uso terapêutico , Antibacterianos/uso terapêutico , Doenças dos Bovinos/tratamento farmacológico , Infecções por Pasteurella/veterinária , Pasteurella multocida/efeitos dos fármacos , Infecções Respiratórias/veterinária , Proteínas Ribossômicas/genética , Espectinomicina/uso terapêutico , Animais , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/microbiologia , China/epidemiologia , Farmacorresistência Bacteriana/genética , Testes de Sensibilidade Microbiana/veterinária , Infecções por Pasteurella/tratamento farmacológico , Infecções por Pasteurella/epidemiologia , Infecções por Pasteurella/microbiologia , Pasteurella multocida/genética , Infecções Respiratórias/tratamento farmacológico , Infecções Respiratórias/epidemiologia , Infecções Respiratórias/microbiologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA