Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 58
Filtrar
1.
Sensors (Basel) ; 21(16)2021 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-34450980

RESUMO

Copper (II) ions have been shown to greatly improve the chemical stability and peroxidase-like activity of gold nanoclusters (AuNCs). Since the affinity between Cu2+ and pyrophosphate (PPi) is higher than that between Cu2+ and AuNCs, the catalytic activity of AuNCs-Cu2+ decreases with the introduction of PPi. Based on this principle, a new colorimetric detection method of PPi with high sensitivity and selectivity was developed by using AuNCs-Cu2+ as a probe. Under optimized conditions, the detection limit of PPi was 0.49 nM with a linear range of 0.51 to 30,000 nM. The sensitivity of the method was three orders of magnitude higher than that of a fluorescence method using AuNCs-Cu2+ as the probe. Finally, the AuNCs-Cu2+ system was successfully applied to directly determine the concentration of PPi in human urine samples.


Assuntos
Ouro , Nanopartículas Metálicas , Colorimetria , Cobre , Difosfatos , Corantes Fluorescentes , Humanos , Limite de Detecção , Peroxidase , Peroxidases , Espectrometria de Fluorescência
2.
Metabolism ; 122: 154840, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34331963

RESUMO

OBJECTIVE: Ferroptosis is indicated in cardiovascular diseases. Given the prominent role of mitophagy in the governance of ferroptosis and our recent finding for FUN14 domain containing 1 (FUNDC1) in obesity anomalies, this study evaluated the impact of FUNDC1 deficiency in high fat diet (HFD)-induced cardiac anomalies. METHODS AND MATERIALS: WT and FUNDC1-/- mice were fed HFD (45% calorie from fat) or low fat diet (LFD, 10% calorie from fat) for 10 weeks in the presence of the ferroptosis inhibitor liproxstatin-1 (LIP-1, 10 mg/kg, i.p.). RESULTS: RNAseq analysis for differentially expressed genes (DEGs) reported gene ontology term related to ferroptosis and mitophagy in obese rat hearts, which was validated in obese rodent and human hearts. Although 10-week HFD intake did not alter global metabolism, cardiac geometry and function, ablation of FUNDC1 unmasked metabolic derangement, pronounced cardiac remodeling, contractile, intracellular Ca2+ and mitochondrial anomalies upon HFD challenge, the effects of which with exception of global metabolism were attenuated or mitigated by LIP-1. FUNDC1 ablation unmasked HFD-evoked rises in fatty acid synthase ACSL4, necroptosis, inflammation, ferroptosis, mitochondrial O2- production, and mitochondrial injury as well as dampened autophagy and DNA repair enzyme 8-oxoG DNA glycosylase 1 (OGG1) but not apoptosis, the effect of which except ACSL4 and its regulator SP1 was reversed by LIP-1. In vitro data noted that arachidonic acid, an ACSL4 substrate, provoked cytochrome C release, cardiomyocyte defect, and lipid peroxidation under FUNDC1 deficiency, the effects were interrupted by inhibitors of SP1, ACSL4 and ferroptosis. CONCLUSIONS: These data suggest that FUNDC1 deficiency sensitized cardiac remodeling and dysfunction with short-term HFD exposure, likely through ACSL4-mediated regulation of ferroptosis.

3.
Chem Commun (Camb) ; 57(58): 7108-7111, 2021 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-34179909

RESUMO

An Fe induction strategy is introduced to achieve template-free synthesis of Co,Fe dual-metal N-codoped hollow porous carbon from zeolitic imidazole frameworks, which is beneficial for the exposure of highly dispersed metal (M)-Nx active sites and enhancement of mass transport, thereby exhibiting a superior electrocatalytic activity (E1/2, 0.86 VRHE).

4.
Artigo em Inglês | MEDLINE | ID: mdl-33861562

RESUMO

A dual substitution strategy is introduced to Co-free layered material LiNi0.5Mn0.5O2 by partially replacing Li and Ni with Na and Al, respectively, to achieve a superior cathode material for lithium ion batteries. Na+ ion functions as a "pillar" and a " cationic barrier" in the lithium layer while Al3+ ion plays an auxiliary role in stabilizing structure and lattice oxygen to improve the electrochemical performance and safety. The stability of lattice oxygen comes from the binding energy between the Ni and O, which is larger due to higher valences of Ni ions, along with a stronger Al-O bond in the crystal structure and the "cationic barrier" effect of Na+ ion at the high-charge. The more stable lattice oxygen reduces the cation disorder in cycling, and Na+ in the Li layer squeezes the pathway of the transition metal from the LiM2 (M = metal) layer to the Li layer, stabilizing the layered crystal structure by inhibiting the electrochemical-driven cation disorder. Moreover, the cathode with Na-Al dual-substitution displays a smaller volume change, yielding a more stable structure. This study unravels the influence of Na-Al dual-substitution on the discharge capacity, midpoint potential, and cyclic stability of Co-free layered cathode materials, which is crucial for the development of lithium ion batteries.

5.
Front Immunol ; 12: 591706, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33717069

RESUMO

Ankylosing spondylitis (AS) is a chronic inflammatory disease that mainly affects the spine. AS is highly associated with the expression of HLA-B27. Up to 95% AS patients are HLA-B27-positive. However, only 1%-2% of the HLA-B27-positive carriers suffer from AS, implying that other factors may also govern the development of AS. Long non-coding RNAs (lncRNAs) can regulate the immune response via their binding proteins. In the present study, we have identified that the levels of lncRNA, LOC645166, in T cells of AS patients were reduced. Overexpression of LOC645166 in Jurkat cells down-regulated the IL-23p19 expression and suppressed the JAK2/STAT3 signaling in response to stimulation by phorbol 12-myristate 13-acetate. Suppression of STAT3 activation by LOC645166 was also observed when Jurkat cells or T cells of AS patient were treated with anti-CD3/CD28 antibodies. In order to explore the role of LOC645166 in the pathogenesis of AS, RNA pull-down assay plus proteomic approach and western blotting were performed and identified that LOC645166 prefers binding the K63-linked polyubiquitin chains. LOC645166 can suppress recruitment of the IKK complex to K63-linked polyubiquitin chains and diminish IKK2 activation, leading to down-regulation of NF-κB activation. Down-regulation of LOC645166 expression in T cells of AS patients up-regulates NF-kB activation via decreasingly impeding recruitment of the IKK complex to K63-linked polyubiquitin chains, allowing AS patients to exhibit more sensitivity to stimulation by the proinflammatory cytokines or by TLR ligands.


Assuntos
Regulação da Expressão Gênica , NF-kappa B/metabolismo , RNA Longo não Codificante/genética , Espondilite Anquilosante/etiologia , Espondilite Anquilosante/metabolismo , Linfócitos T/imunologia , Linfócitos T/metabolismo , Biomarcadores , Citocinas/metabolismo , Suscetibilidade a Doenças , Humanos , Quinase I-kappa B/metabolismo , Mediadores da Inflamação , Transporte Proteico , Transdução de Sinais , Espondilite Anquilosante/patologia , Ubiquitinação
6.
Biochem Biophys Res Commun ; 531(2): 236-241, 2020 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-32800345

RESUMO

Phostensin (PTS) encoded by KIAA1949 is a protein phosphatase 1 (PP1)-binding protein. In order to explore the cellular functions of PTS, we have searched PTS-binding proteins by using co-immunoprecipitation in combination with shotgun proteomics. Here, we report two novel PTS-binding proteins, Eps 15 homology domain-containing protein 1 (EHD1) and EHD4. PTS associated with EHD proteins was also observed in GST pull-down assays. Immunofluorescence microscopy demonstrated that the complex was co-localized at the endocytic vesicles. EHD proteins have been known to play a critical role in regulation of endocytic transport. Overexpression of PTS-ß can attenuate the endocytic trafficking of transferrin.


Assuntos
Proteínas de Ligação a DNA/metabolismo , Proteínas Nucleares/metabolismo , Proteína Fosfatase 1/metabolismo , Proteínas de Transporte Vesicular/metabolismo , Endocitose , Endossomos/metabolismo , Células HeLa , Humanos , Células Jurkat , Cinética , Ligação Proteica , Transferrina/metabolismo
7.
Sensors (Basel) ; 20(11)2020 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-32492975

RESUMO

We developed a label-free, real-time, and highly sensitive nucleic acid biosensor based on fiber optic particle plasmon resonance (FOPPR). The biosensor employs a single-strand deoxyoligonucleotides (ssDNA) probe, conjugated to immobilized gold nanoparticles on the core surface of an optical fiber. We explore the steric effects on hybridization affinity and limit of detection (LOD), by using different ssDNA probe designs and surface chemistries, including diluent molecules of different lengths in mixed self-assembled monolayers, ssDNA probes of different oligonucleotide lengths, ssDNA probes in different orientations to accommodate target oligonucleotides with a hybridization region located unevenly in the strand. Based on the optimized ssDNA probe design and surface chemistry, we achieved LOD at sub-nM level, which makes detection of target oligonucleotides as low as 1 fmol possible in the 10-mL sensor chip. Additionally, the FOPPR biosensor shows a good correlation in determining HLA-B27 mRNA, in extracted blood samples from patients with ankylosing spondylitis (AS), with the clinically accepted real-time reverse transcription-polymerase chain reaction (RT-PCR) method. The results from this fundamental study should guide the design of ssDNA probe for anti-sense sensing. Further results through application to HLA-B27 mRNA detection illustrate the feasibility in detecting various nucleic acids of chemical and biological relevance.


Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , RNA Mensageiro/análise , Espondilite Anquilosante , Sondas de DNA , DNA de Cadeia Simples , Ouro , Antígeno HLA-B27/genética , Humanos , Hibridização de Ácido Nucleico , Espondilite Anquilosante/diagnóstico , Espondilite Anquilosante/genética , Ressonância de Plasmônio de Superfície
8.
ChemSusChem ; 13(6): 1556-1567, 2020 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-31691474

RESUMO

The development of nonprecious metal-nitrogen-carbon (M-N-C) materials with efficient metal utilization and abundant active sites for the oxygen reduction reaction (ORR) is of great significance for fuel cells and metal-air batteries. Ultrasmall 2 D Cox Zn2-x (benzimidazole)4 [Cox Zn2-x (bim)4 ] bimetallic metal-organic framework (MOF) nanosheets (≈2 nm thick) are synthesized by a novel bottom-up strategy and then thermally converted into a core-shell structure of sub-5 nm Co nanodots (NDs) wrapped with 2 to 5 layers of Co,N-codoped graphene (Co@FLG). The size of the Co NDs in Co@FLG could be precisely controlled by the Co/Zn ratio in the Cox Zn2-x (bim)4 nanosheet. As an ORR electrocatalyst, the optimized Co@FLG exhibits an excellent half-wave potential of 0.841 V (vs. RHE), a high limiting current density of 6.42 mA cm-2 , and great stability in alkaline electrolyte. Co@FLG also has great ORR performance in neutral electrolyte, as well as in Mg-air batteries. The experimental studies and DFT calculations reveal that the high performance of Co@FLG is mainly attributed to its great O2 absorptivity, which is endowed by the abundant Co-Nx and pyridinic-N in the FLG shell and the strong electron-donating ability from the Co ND core to the FLG shell. This elevates the eg orbital energy of CoII and lowers the activation energy for breaking the O=O/O-O bonds. This work sheds light on the design and fabrication of 2 D MOFs and MOF-derived M-N-C materials for energy storage and conversion applications.

9.
ACS Appl Mater Interfaces ; 11(1): 930-939, 2019 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-30550259

RESUMO

Carbon materials are most promising candidates for potassium-ion battery (PIB) anodes because of their high electrical conductivities, rational potassium storage capabilities, and low costs. However, the large volume change during the K-ion insertion/extraction and the sluggish kinetics of K-ion diffusion inhibit the development of carbon-based materials for PIBs. Here, under the guidance of density functional theory, N/P-codoped ultrafine (≤20 nm) carbon nanoparticles (NP-CNPs) with an expanded interlayer distance, improved electrical conductivity, shortened diffusion distance of K ions, and promoted adsorption capability toward K ions are synthesized through a facile solvent-free method as a high-performance anode material for PIBs. The NP-CNPs show a high capacity of 270 mA h g-1 at 0.2 A g-1, a remarkable rate capability of 157 mA h g-1 at an extremely high rate of 5.0 A g-1, and an ultralong cycle life with a high capacity of 190 mA h g-1 and a retention of 86.4% at 1.0 A g-1 after 4000 cycles. The potassium storage mechanism and low volume expansion for NP-CNPs are revealed through cyclic voltammetry, in situ Raman, and ex situ XRD. This work paves a new way to design and fabricate carbon-based nanostructures with high reversible capacity, great rate capability, and stable long-term performance.

10.
J Colloid Interface Sci ; 530: 127-136, 2018 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-29966845

RESUMO

The performance of energy storage materials is substantially dependent on their nanostructures. Herein, Ni-1,3,5-benzenetricarboxylate metal-organic frameworks (Ni-BTC MOFs) with different morphologies are controllably synthesized using a facile solvothermal method by simply adjusting the solvent and their electrochemical performance as an anode material for lithium-ion batteries is thoroughly investigated. Among the synthesized Ni-BTC MOFs with different morphologies, a hierarchical mesoporous flower-like Ni-BTC MOF (Ni-BTCEtOH) assembled from two-dimensional nanosheets shows the best electrochemical properties including a high capacity of 1085 mA h g-1 at 100 mA g-1 (358 mA h g-1 at 5000 mA g-1), excellent cycling stability at 1000 mA g-1 for 1000 cycles, and great rate performance, which is superior to most of the reported MOF-based anode materials for lithium-ion batteries. The outstanding electrochemical performance of Ni-BTCEtOH is originated from its unique and stable hierarchical mesoporous morphology with a high specific surface area and improved electrical/ionic conductivity. Moreover, our study demonstrates that the charge-discharge mechanism of the Ni-BTCEtOH electrode involves the insertion/extraction of Li ions to/from the organic moieties in Ni-BTCEtOH during the charge-discharge process without the direct engagement of Ni2+. This work highlights that the nanostructure design is an effective strategy to obtain promising energy storage materials.

11.
Talanta ; 182: 306-313, 2018 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-29501157

RESUMO

A novel dual-target recognition sandwich strategy for selective capture and detection of MCF-7 breast cancer cells based on core-shell magnetic mesoporous silica (Fe3O4@nSiO2@mSiO2@apt) nanoparticles was developed. Fe3O4@nSiO2@mSiO2@apt nanoparticles, which were prepared by a layer-by-layer method and were used for the first time to capture cancer cells, have large surface areas, particularly accessible mesochannels, and good biocompatibility, enabling aptamers to be compactly anchored onto the surface of the core-shell magnetic nanoparticles. A mucin 1 protein (MUC1)-targeted Fe3O4@nSiO2@mSiO2@apt nanoparticle was used as an affinity magnetic isolate material to capture target MCF-7 cells selectively and to reduce interference through affinity interaction between the anti-MUC1 aptamer and the MUC1 protein over-expressed on the surface of the MCF-7 cells. Meanwhile, a folate receptor (FR)-targeted affinity fluorescent probe (FA-BSA-FITC) was developed by coupling folic acid and FITC to the surface of BSA, enabling high sensitivity, selective fluorescent labeling of FR over-expressed MCF-7 cells. A dual-target recognition sandwich assay was developed based on the MUC1-targeted magnetic nanoparticles and the FR-targeted fluorescent probes. Under optimum conditions, a quantitative assay of MCF-7 cells was achieved with a dynamic range of 102-105 cells/mL (R2 = 0.9991). This assay showed high specificity and sensitivity to the target MCF-7 cells. Finally, the proposed strategy could be extended to detect MCF-7 cells in human plasma and whole blood with a recovery range of 86.1-104.0% and a RSD range of 1.2-8.4%, respectively. This indicates that the dual-target recognition method developed in this research exhibits good selectivity, anti-interference capability, and reliability even in plasma and whole blood samples and is more suitable for complex samples than previous targeted assays. Therefore, the approach proposed here may have great potential for early breast cancer diagnosis.


Assuntos
Bioensaio , Neoplasias da Mama/diagnóstico , Separação Celular/métodos , Nanopartículas de Magnetita/química , Células Neoplásicas Circulantes/metabolismo , Aptâmeros de Peptídeos/síntese química , Aptâmeros de Peptídeos/metabolismo , Neoplasias da Mama/metabolismo , Neoplasias da Mama/patologia , Feminino , Óxido Ferroso-Férrico/química , Fluoresceína-5-Isotiocianato/química , Receptores de Folato com Âncoras de GPI/genética , Receptores de Folato com Âncoras de GPI/metabolismo , Ácido Fólico/química , Ácido Fólico/metabolismo , Expressão Gênica , Humanos , Células MCF-7 , Nanopartículas de Magnetita/ultraestrutura , Mucina-1/genética , Mucina-1/metabolismo , Células Neoplásicas Circulantes/imunologia , Células Neoplásicas Circulantes/patologia , Tamanho da Partícula , Ligação Proteica , Transporte Proteico , Soroalbumina Bovina/química , Dióxido de Silício/química
12.
ACS Appl Mater Interfaces ; 10(8): 7031-7042, 2018 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-29338183

RESUMO

The incorporation of oxygen vacancies in anatase TiO2 has been studied as a promising way to accelerate the transport of electrons and Na+ ions, which is important for achieving excellent electrochemical properties for anatase TiO2. However, wittingly introducing oxygen vacancies in anatase TiO2 for sodium-ion anodes by a facile and effective method is still a challenge. In this work, we report an innovative method to introduce oxygen vacancies into the urchin-like N-doped carbon coated anatase TiO2 (NC-DTO) by a facile plasma treatment. The superiorities of the oxygen vacancies combined with the conductive N-doped carbon coating enable the obtained NC-DTO of greatly improved sodium storage performance. When served as the anode for sodium-ion batteries, the NC-DTO electrode shows superior electrochemical performance (capacity: 272 mA h g-1 at 0.25 C, capacity retention: 98.8% after 5000 cycles at 10 C, as well as ultrahigh capacity: 150 mA h g-1 at 15 C). Density functional theory calculations combined with experimental results suggest that considerably improved sodium storage performance of NC-DTO is due to the enhanced electronic conductivity from the N-doped carbon layer as well as narrowed band gap and lowered sodiation energy barrier from the introduction of oxygen vacancies. This work highlights that introducing oxygen vacancies into TiO2 by plasma is a promising method to enhance the electrochemical property of TiO2, which also can be applied to different metal oxides for energy storage devices.

13.
J Hazard Mater ; 347: 431-441, 2018 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-29367154

RESUMO

The positively charged ultrathin g-C3N4 nanosheets are prepared by ultrasonic-assisted exfoliation of the protonated g-C3N4. Compared with the protonated g-C3N4 and exfoliated g-C3N4, the positively charged ultrathin g-C3N4 has abundant functional groups as well as desired dispersibility in deionized water, thus it could serve as a basic building block for designing related heterojunction composites. To take a full advantage of these features, the positively charged ultrathin g-C3N4/MoS2 composites are fabricated through a simple electrostatic adsorption and self-assembly process followed by a hydrothermal method. By loading an appropriate amount of MoS2 on the ultrathin g-C3N4 nanosheets, the as-fabricated composites exhibit considerable improvement on the photocatalytic activities toward the degradation of typical organic pollutants (i.e., methyl orange and phenol) under visible light irradiation. The composite containing 2 wt% MoS2 shows the highest efficiency of about 96.5% for the methyl orange degradation, which is about 3.5 times and 8 times compared to those of the positively charged ultrathin g-C3N4 and bulk g-C3N4, respectively. The superb photocatalytic performance benefits from the unique advantages, including richly available reaction sites, aligned energy levels between g-C3N4 and the MoS2, and efficient electron transfer. This work opens new possibilities for the rational design and construction of the g-C3N4 based composites as highly efficient and stable visible-light driven photocatalysts for the degradation of organic pollutants.

14.
Oncotarget ; 8(39): 66528-66539, 2017 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-29029533

RESUMO

This study was conducted to evaluate the immune impact of mimic endoscopic retrograde appendicitis therapy and appendectomy on rabbits of acute suppurative appendicitis and to determine whether TLR4/MYD88/NF-κB signaling pathway was activated in this process. 48 rabbits were assigned into 4 groups: group I, the mimic endoscopic retrograde appendicitis therapy group; group II, the appendectomy group; group III, the model group; and group IV, the blank group. White blood cells decreased, while levels of C-reactive protein, tumor necrosis factor-α, interleukin-6, interleukin-4, and interleukin-10 increased on the 2nd day in group I and II. IgA in feces decreased at 2 weeks, while fecal microbiota changed at 2 and 4 weeks after appendectomy. CD8+ cells in appendix of group I increased within 8 weeks. Upregulated expression of TLR4, MYD88, and nuclear NF-κB were detected on the 2nd day in group I and II. Mimic endoscopic retrograde appendicitis therapy and appendectomy are effective ways for acute suppurative appendicitis. Mimic endoscopic retrograde appendicitis therapy was more preferable due to its advantage in maintaining intestinal immune function. TLR4/MYD88/NF-κB signaling pathway was activated in acute phase of appendicitis.

15.
ACS Appl Mater Interfaces ; 9(38): 32643-32651, 2017 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-28880065

RESUMO

A novel side-chain-type fluorinated sulfonated polyimide (s-FSPI) membrane is synthesized for vanadium redox batteries (VRBs) by high-temperature polycondensation and grafting reactions. The s-FSPI membrane has a vanadium ion permeability that is over an order of magnitude lower and has a proton selectivity that is 6.8 times higher compared to those of the Nafion 115 membrane. The s-FSPI membrane possesses superior chemical stability compared to most of the linear sulfonated aromatic polymer membranes reported for VRBs. Also, the vanadium redox flow/static batteries (VRFB/VRSB) assembled with the s-FSPI membranes exhibit stable battery performance over 100- and 300-time charge-discharge cycling tests, respectively, with significantly higher battery efficiencies and lower self-discharge rates than those with the Nafion 115 membranes. The excellent physicochemical properties and VRB performance of the s-FSPI membrane could be attributed to the specifically designed molecular structure with the hydrophobic trifluoromethyl groups and flexible sulfoalkyl pendants being introduced on the main chains of the membrane. Moreover, the cost of the s-FSPI membrane is only one-fourth that of the commercial Nafion 115 membrane. This work opens up new possibilities for fabricating high-performance proton-conductive membranes at low costs for VRBs.

16.
ACS Appl Mater Interfaces ; 9(20): 17186-17194, 2017 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-28467838

RESUMO

The development of highly active, environmentally friendly, and long-term stable oxygen evolving catalysts at low costs is critical for efficient and scalable H2 production from water splitting. Here, we report a new and facile one-step electrodeposition of nanocrystalline spinel-type ZnxCo3-xO4 films from an alkaline Zn2+-Co2+-tartrate solution. The electrodeposited ZnxCo3-xO4 electrode could be directly used as the anode for the water electrolysis without any post treatment. The ZnxCo3-xO4 film shows a low and stable overpotential of ∼0.33 V at 10 mA cm-2 (and ∼0.35 V at 20 mA cm-2) for over 10 h and a Tafel slope of ∼39 mV dec-1 toward the oxygen evolution reaction (OER) in 1 M NaOH, comparable to the best performance of the nonprecious OER catalysts reported for alkaline media. The enhanced OER activity of ZnxCo3-xO4 compared to Co3O4 could be attributed to the surface structural modification and higher density of the accessible active Co3+ sites induced by the incorporation of Zn2+. The electrodeposition method in this paper could also be used to synthesize other binary and ternary metal oxide based catalytic electrodes for reactions such as the OER and oxygen reduction reaction (ORR).

17.
Surg Endosc ; 31(5): 2350-2355, 2017 05.
Artigo em Inglês | MEDLINE | ID: mdl-27631315

RESUMO

BACKGROUND: The selection of therapy for benign esophageal lesions depends in part on whether the lesion extends to or through the esophageal muscle wall. The advent of endoscopic dissection of deep lesions has made this distinction important in the choice between different forms of advanced endoscopic therapy. The goal of this study was to evaluate esophageal insufflation computed tomography (EICT) for the diagnosis and management of esophageal submucosal tumors (SMTs). METHODS: Between April 2011 and May 2013 at the Second Affiliated Hospital of Harbin Medical University, 27 patients with esophageal SMTs diagnosed by gastroscopy were studied observationally. Entry criteria included tumors larger than 0.5 cm. We compared endoscopic ultrasound (EUS) and EICT to assess lesion depth and the relationship between the submucosal lesion and the esophageal wall using the resected lesion as the gold standard. RESULTS: Twenty-seven esophageal SMTs were evaluated. EUS and EICT accurately identified nine as superficial to the muscularis propria. EICT correctly identified the relation of the tumor extension and the outer esophageal wall in all 18 lesions that originated from the muscularis propria; only nine were correctly assessed by EUS (P < 0.001). CONCLUSIONS: EICT enables improved judgment of the relation of esophageal lesions and the esophageal-mediastinal border. We propose EICT as a new, safe, effective, useful, simple and high-tolerance method for assessing the depth and relationships of esophageal submucosal lesions.


Assuntos
Endossonografia/métodos , Neoplasias Esofágicas/diagnóstico por imagem , Neoplasias Esofágicas/terapia , Gastroscopia/métodos , Insuflação/métodos , Tomografia Computadorizada por Raios X , Adulto , Idoso , Dissecação/métodos , Neoplasias Esofágicas/patologia , Feminino , Mucosa Gástrica/diagnóstico por imagem , Mucosa Gástrica/cirurgia , Humanos , Masculino , Pessoa de Meia-Idade , Músculo Liso/diagnóstico por imagem , Músculo Liso/patologia , Músculo Liso/cirurgia
18.
Mediators Inflamm ; 2017: 4016802, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29463951

RESUMO

Ankylosing spondylitis (AS) is highly associated with the expression of human leukocyte antigen-B27 (HLA-B∗27). HLA-B∗27 heavy chain (B27-HC) has an intrinsic propensity to fold slowly, leading to the accumulation of the misfolded B27-HC in the endoplasmic reticulum (ER) and formation of the HLA-B∗27 HC homodimer, (B27-HC)2, by a disulfide linkage at Cys-67. (B27-HC)2 displayed on the cell surface can act as a ligand of the killer-cell Ig-like receptor (KIR3DL2). (B27-HC)2 binds to KIR3DL2 of NK and Th17 cells and activates both cells, resulting in the activation of the IL-23/IL-17 axis to launch the inflammatory reaction in AS patients. However, activation of the IL-23/IL-17 axis originally derived from the HLA-B∗27 misfolding in the ER needs to be characterized. In this study, we delivered two HLA-B∗27-binding peptides, KRGILTLKY and SRYWAIRTR, into the ER by using a tat-derived peptide (GRKKRRQRRR)-His6-ubiquitin (THU) vehicle. Both peptides are derived from the human actin and nucleoprotein of influenza virus, respectively. Our results demonstrated that targeted delivery of both HLA-B∗27-binding peptides into the ER can promote the HLA-B∗27 folding, decrease the levels of (B27-HC)2, and suppress the activation of the IL-23/IL-17 axis in response to lipopolysaccharide. Our findings can provide a new therapeutic strategy in AS.


Assuntos
Retículo Endoplasmático/metabolismo , Antígeno HLA-B27/metabolismo , Interleucina-17/metabolismo , Interleucina-23/metabolismo , Peptídeos/metabolismo , Espondilartrite/metabolismo , Western Blotting , Linhagem Celular , Células Cultivadas , Citometria de Fluxo , Humanos
20.
Artigo em Chinês | MEDLINE | ID: mdl-26696197

RESUMO

OBJECTIVE: To investigate the onset time and efficacy of sublingual immunotherapy (SLIT) with Dermatophagoides farinae drops in children with house dust mites (HDM)-induced allergic rhinitis (AR). METHODS: One hundred and forty three children with perennial moderate to severe HDM-induced AR were treated by SLIT with standardized Dermatophagoides farinae extract. One hundred children who finally completed two years treatment were divided into two groups according to the age: younger children group (aged 4-8 years, n = 52) and older children group (aged 9-14 years, n = 48). Respectively, Each children was assessed before and after 1st, 2nd, 3rd, 6th, 12th, 24th months of the treatment. Total nasal symptom score (TNSS), total medication score (TMS) and visual analogue scale (VAS) were evaluated at each visit. All clinical data were analyzed retrospectively with the SPSS 19.0 software. RESULTS: TNSS, TMS and VAS of two groups decreased significantly after three months of the treatment compared with before (younger children group: Z value was -3.843, -3.534, -3.940, older children group: Z value was -3.938, -3.405, -3.953, all P < 0.05). TNSS and VAS of younger children group decreased significantly after two months of the treatment compared with before (6.4 ± 1.6, 5.3 ± 1.4 vs 8.6 ± 1.2, 7.9 ± 1.6, Z value was -3.843, -3.940, both P < 0.05). Five children (5%) experienced local adverse events and 2 children (2%) experienced mild systemic adverse events. No severe adverse events happened during the treatment. CONCLUSIONS: SLIT with Dermatophagoides farinae drops is an efficient and safe treatment for children with HDM-induced AR. Its onset of action can be observed as early as 3 months after treatment.


Assuntos
Alérgenos/administração & dosagem , Antígenos de Dermatophagoides/administração & dosagem , Rinite Alérgica Perene/tratamento farmacológico , Imunoterapia Sublingual , Administração Sublingual , Adolescente , Animais , Criança , Pré-Escolar , Dermatophagoides farinae , Humanos , Estudos Retrospectivos , Software , Resultado do Tratamento
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...