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1.
Small ; : e2206947, 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36631255

RESUMO

Two large barriers are impeding the wide implementation of electric vehicles, namely driving-range and cost, primarily due to the low specific energy and high cost of mono-valence cathodes used in lithium-ion batteries. Iron is the ideal element for cathode materials considering its abundance, low cost and toxicity. However, the poor reversibility of (de)lithiation and low electronic conductivity prevent iron-based high specific energy multi-valence conversion cathodes from practical applications. In this work, a sustainable FeOF nanocomposite is developed with extraordinary performance. The specific capacity and energy reach 621 mAh g-1 and 1124 Wh kg-1 with more than 100 cycles, which triples the specific capacity, and doubles the specific energy of current mono-valence intercalation LiCoO2 . This is the result of an effective approach, combing the nanostructured FeOF with graphene, realized by making the (de)lithiation reversible by immobilizing FeOF nanoparticles and the discharge products over the graphene surface and providing the interparticle electric conduction. Importantly, it demonstrates that introducing small amount of graphene can create new materials with desired properties, opening a new avenue for altering the (de)lithiation process. Such extraordinary performance represents a significant breakthrough in developing sustainable conversion materials, eventually overcoming the driving range and cost barriers.

2.
Anim Sci J ; 93(1): e13792, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36477978

RESUMO

Heat stress is considered as a limiting factor for sheep production; it is necessary to screen for sheep breeds with heat tolerance. This study was to compare the serum metabolomes of Hu sheep and Dorper sheep and identify potential biomarkers related to heat stress. The results revealed that the respiratory rate, heart rate, and rectal temperature of Dorper sheep were significantly higher than those of Hu sheep. Compared to Dorper sheep, the serum activities of total antioxidant capacity and glutathione peroxidase in Hu sheep were significantly higher, while the concentration of malondialdehyde was lower. Metabolomics analysis identified 107 differential serum metabolites. The pathways enriched from the altered serum metabolites between the two breeds were mainly involved in protein metabolism, carbohydrate metabolism, and lipid metabolism. The levels of antioxidant- and energy-related metabolites were higher in the serum of Hu sheep than that of Dorper sheep; however, the levels of lipid catabolism- and inflammation-related were higher in the serum of Dorper sheep. The results indicate that Hu sheep had better heat stress resistance capability than Dorper sheep. Moreover, high levels of metabolites in the serum of Hu sheep are potential biomarkers for heat stress tolerance, including l-methionine, s-adenosylmethionine, and nicotinuric acid.


Assuntos
Termotolerância , Ovinos , Animais , Metabolômica , Frequência Cardíaca
3.
Polymers (Basel) ; 14(23)2022 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-36501595

RESUMO

A full-scale model for predicting low-velocity impact (LVI) damage and compression after impact (CAI) strength was established based on a subroutine of the material constitutive relationship and the cohesive elements. The dynamic responses of the laminate under impact load and damage propagation under a compressive load were presented. The influences of impact energy and ply thickness on the impact damage and the CAI strength were predicted. The predicted results were compared with the experimental ones. It is shown that the predicted value of the CAI strength is in good agreement with the experimental result. As the impact energy reaches a certain value, the CAI strength no longer decreases with the increase in the impact energy. Decreasing the ply thickness can effectively improve the damage resistance and CAI strength.

4.
RSC Adv ; 12(42): 27463-27472, 2022 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-36276029

RESUMO

Magnetic nanoparticles are attracting significant attention for their wide application as biomaterials and magnetic storage materials. As an environmentally friendly adhesive, reactive polyurethane hot-melt adhesive (PUR) is a biocompatible polymer with a wide range of applications. In this paper, chitosan (CS)-surface-modified magnetic Fe3O4 nanoparticles were synthesized by the sol-gel method. Surface modification of the Fe3O4 nanoparticles with CS enhanced their mechanical properties in PUR. The nanoparticles were characterized by Fourier transform infrared (FTIR) and X-ray diffraction (XRD) analyses, while their surface morphology was elucidated using scanning electron microscopy (SEM) and projection electron microscopy (TEM) techniques. Subsequently, PUR/CS-Fe3O4 magnetic nanocomposite films were prepared using an in situ method, wherein different amounts of CS-surface-modified magnetic Fe3O4 nanoparticles were doped into the PUR and coated on the films. The thermal, UV resistance and mechanical properties of the PUR/CS-Fe3O4 magnetic nanocomposite films were investigated by TGA, UV spectrometer and tensile testing. CS-Fe3O4 nanoparticles were successfully prepared using the sol-gel method and CS to modify the surface of the Fe3O4 nanoparticles. The results show that the mechanical properties and UV resistance of PUR/CS-Fe3O4 magnetic nanocomposites are improved by almost 50%, so the constructed PUR/CS-Fe3O4 magnetic nanocomposites have good UV-resistant properties and mechanical properties. The as-synthesized CS-Fe3O4 magnetic nanocomposites show great potential for application to mechanical and textile development.

5.
Cell Biosci ; 12(1): 74, 2022 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-35642067

RESUMO

BACKGROUND: The global population of older individuals is growing, and ageing is a key risk factor for atherosclerotic cardiovascular diseases. Abnormal accumulation of senescent cells can cause potentially deleterious effects on the organism with age. As a vital marker of cellular senescence, the senescence-associated secretory phenotype (SASP) is a novel mechanism to link cellular senescence with atherosclerosis. MAIN BODY: In this review, we concretely describe the characteristics of the SASP and its regulation mechanisms. Importantly, we provide novel perspectives on how the SASP can promote atherosclerosis. The SASP from different types of senescent cells have vital roles in atherosclerosis progression. As a significant mediator of the harmful effects of senescent cells, it can play a pro-atherogenic role by producing inflammation and immune dysfunction. Furthermore, the SASP can deliver senescence signals to the surrounding vascular cells, gradually contributing to the development of atherosclerosis. Finally, we focus on a variety of novel therapeutic strategies aimed to reduce the burden of atherosclerosis in elderly individuals by targeting senescent cells and inhibiting the regulatory mechanisms of the SASP. CONCLUSION: This review systematically summarizes the multiple roles of the SASP in atherosclerosis and can contribute to the exploration of new therapeutic opportunities.

6.
CNS Neurosci Ther ; 28(6): 953-963, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35322553

RESUMO

AIMS: Inflammatory processes induced by leukocytes are crucially involved in the pathophysiology of acute ischemic stroke. This study aimed to elucidate the inflammatory mechanism of long non-coding RNA (lncRNA) H19-mediated regulation of C1q and tumor necrosis factor 6 (C1QTNF6) by sponging miR-29b in leukocytes during ischemic stroke. METHODS: H19 and miR-29b expression in leukocytes of patients with ischemic stroke and rats with middle cerebral artery occlusion were measured by real-time polymerase chain reaction. H19 siRNA and miR-29b antagomir were used to knock down H19 and miR-29b, respectively. We performed in vivo and in vitro experiments to determine the impact of H19 and miR-29b on C1QTNF6 expression in leukocytes after ischemic injury. RESULTS: H19 and C1QTNF6 upregulation, as well as miR-29b downregulation, was detected in leukocytes of patients with stroke. Moreover, miR-29b could bind C1QTNF6 mRNA and repress its expression, while H19 could sponge miR-29b to maintain C1QTNF6 expression. C1QTNF6 overexpression promoted the release of IL-1ß and TNF-α in leukocytes, further exacerbated blood-brain barrier disruption, and aggravated the cerebral ischemic injury. CONCLUSIONS: Our findings confirm that H19 promotes leukocyte inflammation by targeting the miR-29b/C1QTNF6 axis in cerebral ischemic injury.


Assuntos
AVC Isquêmico , MicroRNAs , RNA Longo não Codificante , Animais , Colágeno , Humanos , Inflamação/genética , Inflamação/metabolismo , Leucócitos/metabolismo , MicroRNAs/genética , MicroRNAs/metabolismo , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , Ratos , Fator de Necrose Tumoral alfa
7.
Membranes (Basel) ; 12(3)2022 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-35323812

RESUMO

Diamond films were deposited on silicon nitride (Si3N4) substrates with three different roughnesses using the method of hot-filament chemical vapor deposition (HFCVD). The tribological properties of the film were studied by changing the deposition time, deposition distance, and methane (CH4) concentration. The friction coefficient, delamination threshold load, and wear rate of the diamond films were tested and calculated using the reciprocating friction and wear test under dry friction conditions. The results show that, when the deposition time is 12 h, the bonding force of the film is the lowest and the friction coefficient is the largest (0.175, 0.438, and 0.342); the deposition distance has little effect on the friction performance. The friction coefficients (0.064, 0.107, and 0.093) of nano-diamond films (NCD) prepared at a 40 sccm CH4 concentration are smaller than those of micro-diamond films (MCD) prepared at a 16 sccm CH4 concentration. The load thresholds before delamination of Ra 0.4 µm substrate diamond film are as high as 40 N and 80 N, whereas the diamond films deposited on Ra 0.03 µm substrates have lower wear rates (4.68 × 10-4 mm3/mN, 5.34 × 10-4 mm3/mN) and low friction coefficients (0.119, 0.074, 0.175, and 0.064). Within a certain load range, the deposition of a diamond film on a Ra 0.03 µm Si3N4 substrate significantly reduces the friction coefficient and improves wear resistance. Diamond film can improve the friction performance of a workpiece and prolong its service life.

8.
Anim Biosci ; 35(10): 1535-1544, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35240019

RESUMO

OBJECTIVE: The objective of this study was to investigate the effects of decreasing dietary crude protein content on rumen fermentation, mictobiota, and metabolites in goats. METHODS: In an 84-day feeding trial, a total of twelve male Anhui white goat kids with initial body weight 15.9±1.13 kg were selected and randomly classified into two groups, feeding a normal crude protein diet (14.8% CP, NCP) or a low crude protein diet (12.0% CP, LCP). At the end of the experimental trial (on day 84), six animals were randomly selected from each group and were slaughtered to collect rumen fluid samples for the analysis of rumen fermentation parameters, microbiome, and metabolome. RESULTS: The concentrations of ammonia-nitrogen, total volatile fatty acid, acetate, and propionate were decreased (p<0.05) in the LCP group in comparison with those in the NCP group. The abundances of genera Prevotella, Campylobacter, Synergistetes, and TG5, which were associated with nitrogen metabolism, were lower (p<0.05) in the LCP group compared with those in the NCP group. The levels of 78 metabolites (74 decreased, 4 increased) in the rumen fluid were altered (p<0.05) by the treatment. Most of the ruminal metabolites that showed decreased levels in the LCP group were substrates for microbial protein synthesis. Metabolic pathway analysis showed that vitamin B6 metabolism was significantly different (p<0.05) in rumen fluid between the two treatments. CONCLUSION: Decreased dietary protein level inhibited rumen fermentation through microbiome and metabolome shifts in goat kids. These results enhance our understanding of ruminal bacteria and metabolites of goat fed a low protein diet.

9.
Nat Commun ; 13(1): 478, 2022 01 25.
Artigo em Inglês | MEDLINE | ID: mdl-35078991

RESUMO

N6-methyladenosine (m6A) plays important role in lineage specifications of embryonic stem cells. However, it is still difficult to systematically dissect the specific m6A sites that are essential for early lineage differentiation. Here, we develop an adenine base editor-based strategy to systematically identify functional m6A sites that control lineage decisions of human embryonic stem cells. We design 7999 sgRNAs targeting 6048 m6A sites to screen for m6A sites that act as either boosters or barriers to definitive endoderm specification of human embryonic stem cells. We identify 78 sgRNAs enriched in the non-definitive endoderm cells and 137 sgRNAs enriched in the definitive endoderm cells. We successfully validate two definitive endoderm promoting m6A sites on SOX2 and SDHAF1 as well as a definitive endoderm inhibiting m6A site on ADM. Our study provides a functional screening of m6A sites and paves the way for functional studies of m6A at individual m6A site level.


Assuntos
Adenosina/análogos & derivados , Diferenciação Celular , Linhagem da Célula , Endoderma/citologia , Regulação da Expressão Gênica no Desenvolvimento , Células-Tronco Embrionárias Humanas/metabolismo , Fatores de Transcrição SOXB1/metabolismo , Adenosina/genética , Adenosina/metabolismo , Adrenomedulina/genética , Adrenomedulina/metabolismo , Sistemas CRISPR-Cas/genética , Células Cultivadas , Endoderma/metabolismo , Humanos , Proteínas/genética , Proteínas/metabolismo , Fatores de Transcrição SOXB1/genética
10.
J Interpers Violence ; 36(7-8): 3710-3731, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-29806559

RESUMO

At least some forms of interpersonal violence could follow from a vision of the self as a fierce, dominant creature. This should be particularly true when psychopathic (more proactive, less reactive) tendencies are involved. Possible relations of this type were examined in two studies (total N = 278) in which college student samples were presented with a new, structured version of an old projective test typically used in psychotherapy contexts. Participants were presented with predator-prey animal pairs (e.g., lion-zebra) that were not explicitly labeled as such. For each pair, the person was asked to choose the animal that they would more prefer to be. Participants who desired to be predator animals more often, on this Revised Animal Preference Test (RAPT), tended toward psychopathy to a greater extent. In Study 1, such relations were manifest in terms of correlations with psychopathic traits and with an interpersonal style marked by hostile dominance. Further analyses, though, revealed that predator self-identifications were more strongly related to primary psychopathy than secondary psychopathy. Study 2 replicated the interpersonal style correlates of the RAPT. In addition, photographs were taken of the participants in the second study and these photographs were rated for apparent hostility and dominance. As hypothesized, participants who wanted to be predator animals to a greater extent also appeared more hostile and dominant in their nonverbal behaviors. These studies suggest that projective preferences can be assessed in a reliable manner through the use of standardizing procedures. Furthermore, the studies point to some of the motivational factors that may contribute to psychopathy and interpersonal violence.


Assuntos
Transtorno da Personalidade Antissocial , Violência , Animais , Hostilidade , Humanos
11.
Med Sci Monit ; 26: e921463, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32606286

RESUMO

BACKGROUND Iron deficiency anemia (IDA) is a global public health problem. This study aimed to analyze the social determinants of IDA in infants living in Shanghai, China, between 2010-2015. MATERIAL AND METHODS Data were analyzed retrospectively from the Shanghai Children's Health Check Record system in the Tangqiao Community Health Service Center for infants aged <24 months between January 1, 2010, and December 31, 2015. A study group was identified with IDA, and an age-matched and gender-matched healthy control group was identified. RESULTS The five-year prevalence rate of IDA was 11.49% (150/1305). Infants with IDA had significantly lower birth weights (3228.720±456.200 gm vs. 3376.870±393.719 gm; P<0.01), a higher percentage of premature births (10.67% vs. 1.33%; P<0.001), a longer exclusive breastfeeding period (10.63±4.844 months vs. 7.08±5.039 months; P<0.001), a higher rate of exclusive breastfeeding within four months after birth (P<0.001), a later start for complementary feeding (7.32±1.633 months vs. 6.93±1.794 months; P<0.05), and a higher rate of starting complementary feeding after 6 months of age (P<0.05). There were no significant differences between the two groups for maternal child-bearing age, mode of delivery, infant birth height, and birth rank. CONCLUSIONS The introduction of an iron-fortified formula for exclusively breastfed infants at 4 months of age, starting complementary feeding promptly before 6 months of age, and improving perinatal care for pregnant women to avoid premature birth and reduce low birth weight may be effective measures to prevent IDA.


Assuntos
Anemia Ferropriva/epidemiologia , Anemia Ferropriva/etiologia , Aleitamento Materno , Estudos de Casos e Controles , China/epidemiologia , Feminino , Humanos , Lactente , Fenômenos Fisiológicos da Nutrição do Lactente , Recém-Nascido de Baixo Peso , Recém-Nascido , Ferro/metabolismo , Masculino , Prevalência , Estudos Retrospectivos , Fatores de Risco , População Urbana
12.
Aging (Albany NY) ; 12(2): 1747-1759, 2020 01 29.
Artigo em Inglês | MEDLINE | ID: mdl-32003754

RESUMO

Inflammation, especially involving the NLRP3 inflammasome, is critical to atherosclerotic plaque formation. Enhanced autophagy can inhibit the development of atherosclerosis, and recent studies have revealed that NLRP3 inflammasome can be degraded by autophagy in atherosclerosis. In the present study, we established a foam-cell model to investigate the impact of oxidized low density lipoproteins (ox-LDLs) on autophagy and the inflammasome in atherosclerosis-related inflammation. We observed that ox-LDLs activated NLRP3 inflammasomes in macrophages and restricted autophagy in a time-and dose-dependent manner. We further observed through immunoprecipitation and siRNA knockdown that autophagic degradation of the NLRP3 inflammasome is dependent on K63 polyubiquitation of its NLRP3 subunit and subsequent binding by the adaptor protein p62. Our findings uncover a mechanism by which autophagy inhibits inflammation in atherosclerosis and the role of K63 in that process.


Assuntos
Autofagia , Inflamassomos/metabolismo , Lipoproteínas LDL/metabolismo , Macrófagos/imunologia , Macrófagos/metabolismo , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Ubiquitina/metabolismo , Linhagem Celular Tumoral , Relação Dose-Resposta a Droga , Humanos , Lipoproteínas LDL/farmacologia , Ligação Proteica , Proteólise , Ubiquitinação
13.
Infect Drug Resist ; 12: 3259-3267, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31802917

RESUMO

BACKGROUND: Parents play an important role in the management of their children's illnesses, including in decisions concerning how and whether to use antibiotics. This study aims to assess the knowledge and attitude towards antibiotics usage among young parents from different areas in Shanghai and to identify contributing factors of antibiotics usage that are comprehensible for parents. METHODS: A cross-sectional study was conducted at vaccination clinics of three community health centers from April to June 2017. Primary guardians who took children for vaccination under the national expanded immunization program were invited to participate. RESULTS: Of the 1368 young parents interviewed, 78.4% of them have taken their children for medical treatment, and 68.9% of their children have taken antibiotics. Parents with education level of senior high school (OR=1.850, 95% CI: 1.214-2.818), or university/college, or above (OR=3.950, 95% CI: 2.604-5.991), and parents with high-income (OR=1.360, 95% CI: 1.013-1.827) have better comprehension of antibiotics. It is found that parents who deny the existence of antibiotic abuse in China (OR=1.857, 95% CI: 1.100-3.135), who have lower income (OR=1.536, 95% CI: 1.074-2.197) and who have antibiotics prescribed by doctors (OR=1.473, 95% CI: 1.048-2.070) are associated with those incorrect practices. CONCLUSION: Lack of knowledge and poor perception of antibiotics usage are found among young parents in Shanghai. Intervention should be conducted to publicize the rational use of antibiotics, reduce the rate of inappropriate behaviors and limit the sale of antibiotics.

14.
Sci Technol Adv Mater ; 20(1): 401-411, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31105803

RESUMO

Deformation microstructure of orthorhombic-α" martensite in a Ti-7.5Mo (wt.%) alloy was investigated by tracking a local area of microstructure using scanning electron microscopy, electron back-scattered diffraction, and transmission electron microscopy. The as-quenched α" plates contain {111}α"-type I transformation twins generated to accommodate transformation strain from bcc-ß to orthorhombic-α" martensite. Tensile deformation up to strain level of 5% induces {112}α"-type I deformation twins. The activation of {112}α"-type I deformation twinning mode is reported for the first time in α" martensite in ß-Ti alloys. {112}α"-type I twinning mode was analyzed by the crystallographic twinning theory by Bilby and Crocker and the most possible mechanism of atomic displacements (shears and shuffles) controlling the newly reported {112}α"-type I twinning were proposed.

15.
BMC Bioinformatics ; 20(Suppl 7): 200, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31074373

RESUMO

BACKGROUND: Transcription factors (TFs) play important roles in the regulation of gene expression. They can activate or block transcription of downstream genes in a manner of binding to specific genomic sequences. Therefore, motif discovery of these binding preference patterns is of central significance in the understanding of molecular regulation mechanism. Many algorithms have been proposed for the identification of transcription factor binding sites. However, it remains a challengeable problem. RESULTS: Here, we proposed a novel motif discovery algorithm based on support vector machine (MD-SVM) to learn a discriminative model for TF binding sites. MD-SVM firstly obtains position weight matrix (PWM) from a set of training datasets. Then it translates the MD problem into a computational framework of multiple instance learning (MIL). It was applied to several real biological datasets. Results show that our algorithm outperforms MI-SVM in terms of both accuracy and specificity. CONCLUSIONS: In this paper, we modeled the TF motif discovery problem as a MIL optimization problem. The SVM algorithm was adapted to discriminate positive and negative bags of instances. Compared to other svm-based algorithms, MD-SVM show its superiority over its competitors in term of ROC AUC. Hopefully, it could be of benefit to the research community in the understanding of molecular functions of DNA functional elements and transcription factors.


Assuntos
Algoritmos , Motivos de Nucleotídeos , Máquina de Vetores de Suporte , Fatores de Transcrição/metabolismo , Sítios de Ligação , Humanos , Ligação Proteica
16.
BMJ Open ; 9(3): e021980, 2019 03 03.
Artigo em Inglês | MEDLINE | ID: mdl-30833308

RESUMO

OBJECTIVES: To understand the career orientation and impact factors of general practitioners (GPs) in Shanghai. DESIGN: A cross-sectional study was carried out from August 2014 to December 2015 using the Career Orientations Inventory (short form). SETTING AND PARTICIPANTS: We conducted a cross-sectional study of 1159 GPs, of which 1067 (92.06%) completed the assessment tools, from 223 community healthcare centres in Shanghai RESULTS: The top career orientation was organisational job security (71.60%), followed by technical competence (12.18%). Compared with female GPs, male GPs scored higher in managerial competence (p<0.001), creativity and entrepreneurship (p<0.001), and lower in organisational job security (p=0.034). Compared with GPs younger than 40, those aged 40 years and older scored higher in sense of service (p=0.003) and lower in autonomy (p=0.022) and lifestyle integration (p=0.039). Compared with GPs with lower education levels, those with at least a bachelor's degree scored higher in managerial competence (p=0.001 and autonomy (p=0.025). In addition, those with fewer than 10 years of work experience scored higher in managerial competence (p=0.008) and scored lower in geographical security (p=0.032) compared with GPs with longer durations of work experience. GPs with senior professional positions scored higher in technical competence (p=0.012) compared with those with lower professional positions. CONCLUSIONS: The search for job stability and the lack of career prospect planning are two factors that impact community GP growth. Individualised skills training and career development planning should be provided to GPs of specific genders, educational background and vocational competence, in order to enhance their job satisfaction and service quality, thereby achieving retention of this staff group.


Assuntos
Escolha da Profissão , Clínicos Gerais , Gestão de Recursos Humanos , Adulto , China , Competência Clínica , Estudos Transversais , Feminino , Clínicos Gerais/psicologia , Clínicos Gerais/normas , Clínicos Gerais/estatística & dados numéricos , Humanos , Satisfação no Emprego , Masculino , Pessoa de Meia-Idade , Gestão de Recursos Humanos/métodos , Gestão de Recursos Humanos/normas , Atenção Primária à Saúde/organização & administração , Atenção Primária à Saúde/estatística & dados numéricos
17.
Carbohydr Polym ; 206: 110-120, 2019 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-30553304

RESUMO

Atherosclerosis (AS) is a chronic inflammatory disease, and many factors are implicated in its progression. This work aims to study the preventive effects and the regulatory mechanism of low-molecular-weight fucoidan (LMWF), which is obtained from Saccharina japonica, on the development of AS. Serum biochemical indices and pathological analyses were determined via ELISA, hematoxylin and eosin (HE) and Oil Red O staining. These results indicated that LMWF ameliorated areas with atherosclerotic lesions and had a significant antioxidant effect. Anticoagulant assays showed that LMWF might serve as a potential anticoagulation drug for inhibiting the formation of atherothrombosis. Double immunofluorescence staining illustrated that LMWF inhibited both SMC proliferation and migration and macrophage formation and differentiation. A molecular biology experiment showed that LMWF exhibited an apparent regulatory effect on various signaling pathways. In conclusion, our results revealed that LMWF mitigated AS in the ApoE (-/-) mouse model by activating multiple signal pathways.


Assuntos
Aterosclerose/tratamento farmacológico , Polissacarídeos/uso terapêutico , Transdução de Sinais/efeitos dos fármacos , Animais , Anticoagulantes/química , Anticoagulantes/isolamento & purificação , Anticoagulantes/uso terapêutico , Antioxidantes/química , Antioxidantes/isolamento & purificação , Antioxidantes/uso terapêutico , Apolipoproteínas E/genética , Sequência de Bases , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Técnicas de Inativação de Genes , Hipolipemiantes/química , Hipolipemiantes/isolamento & purificação , Hipolipemiantes/uso terapêutico , Macrófagos/efeitos dos fármacos , Masculino , Camundongos Endogâmicos C57BL , Peso Molecular , Miócitos de Músculo Liso/efeitos dos fármacos , Feófitas/química , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Probucol/uso terapêutico
18.
Phys Chem Chem Phys ; 20(21): 14947, 2018 05 30.
Artigo em Inglês | MEDLINE | ID: mdl-29780996

RESUMO

Correction for 'Insights into the enhanced Ce[triple bond, length as m-dash]N triple bond in the HCe[triple bond, length as m-dash]N molecule' by Zhen Pu et al., Phys. Chem. Chem. Phys., 2017, 19, 8216-8222.

19.
Anat Rec (Hoboken) ; 301(6): 1115-1125, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29350822

RESUMO

Rotenone is a common pesticide and has been reported as one of the risk factors for Parkinson disease. Rotenone can cause neuronal death or apoptosis through inducing oxidative injury and inhibiting mitochondrial function. As a natural polyphenolic compound, resveratrol possesses the antioxidant capacity and neuroprotective effect. However, the mechanism underlying the neuroprotective effect of resveratrol against rotenone-induced neurotoxicity remains elusive. Here, we treated PC12 cells with rotenone to induce neurotoxicity, and the neurotoxic cells were subjected to resveratrol treatment. The CCK8 and LDH activity assays demonstrated that resveratrol could suppress neurotoxicity induced by rotenone (P < 0.01). The DCFH-DA assay indicated that resveratrol reduced the production of reactive oxygen species (ROS). JC-1 and Hoechst 33342/PI staining revealed that resveratrol attenuated mitochondrial dysfunction and cell apoptosis. Moreover, resveratrol reversed rotenone-induced decrease in SIRT1 expression and Akt1 phosphorylation (P < 0.05). Furthermore, when the SIRT1 and Akt1 activity was inhibited by niacinamide and LY294002, respectively, the neuroprotective effect of resveratrol was remarkably attenuated, which implied that SIRT1 and Akt1 could mediate this process and may be potential molecular targets for intervening rotenone-induced neurotoxicity. In summary, our study demonstrated that resveratrol reduced rotenone-induced oxidative damage, which was partly mediated through activation of the SIRT1/Akt1 signaling pathway. Our study launched a promising avenue for the potential application of resveratrol as a neuroprotective therapeutic agent in Parkinson disease. Anat Rec, 301:1115-1125, 2018. © 2018 Wiley Periodicals, Inc.


Assuntos
Neurônios/efeitos dos fármacos , Fármacos Neuroprotetores/farmacologia , Resveratrol/farmacologia , Rotenona/farmacologia , Transdução de Sinais/efeitos dos fármacos , Animais , Antioxidantes/farmacologia , Apoptose/efeitos dos fármacos , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Neurônios/metabolismo , Células PC12 , Proteínas Proto-Oncogênicas c-akt/metabolismo , Ratos , Espécies Reativas de Oxigênio/metabolismo , Sirtuína 1/metabolismo
20.
Cell Physiol Biochem ; 43(3): 1220-1234, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28982096

RESUMO

BACKGROUND/AIMS: Bleomycin is a clinically used anti-cancer drug that produces DNA breaks once inside of cells. However, bleomycin is a positively charged molecule and cannot get inside of cells by free diffusion. We previously reported that the cell surface negatively charged glycosaminoglycans (GAGs) may be involved in the cellular uptake of bleomycin. We also observed that a class of positively charged small molecules has Golgi localization once inside of the cells. We therefore hypothesized that bleomycin might perturb Golgi-operated GAG biosynthesis. METHODS: We used stable isotope labeling coupled with LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells. To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis. RESULTS: we discovered that heparan sulfate GAG was significantly undersulfated and the quantity and disaccharide compositions of GAGs were changed in bleomycin-treated cells in a concentration- and time-dependent manner. We revealed that bleomycin-induced cytotoxicity was directly related to cell surface GAGs. CONCLUSION: GAGs were targeted by bleomycin both at cell surface and at Golgi. Thus, GAGs might be the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism.


Assuntos
Bleomicina/química , Sulfatos de Condroitina/química , Heparitina Sulfato/química , Animais , Antipirina/análogos & derivados , Antipirina/química , Bleomicina/toxicidade , Células CHO , Sulfatos de Condroitina/análise , Sulfatos de Condroitina/metabolismo , Cromatografia Líquida de Alta Pressão , Cricetinae , Cricetulus , Deutério/química , Edaravone , Células HCT116 , Células HT29 , Heparitina Sulfato/análise , Heparitina Sulfato/metabolismo , Humanos , Marcação por Isótopo , Espectrometria de Massas
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