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1.
Food Chem ; 370: 131002, 2022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-34500296

RESUMO

The effects of tempered procedures (well and under-tempered) on the crystalline behaves of cocoa butter were elaborated through detecting crystalline structure and compositions of crystals located at different positions of cocoa butter products in this study. The under-tempered products couldn't form crystalline structures as uniform as the well-tempered ones, whose internal contained more low saturated triacylglycerol and structurally unstable crystals. The low saturated triacylglycerol further created the diverse microstructure and thermal properties between center and outer part of cocoa butter products. During storage, the concentration differences drive migration of low saturated triacylglycerol from center to outer part of the product. Although this reduces the differences in triacylglycerol composition, it results in the polymorphism conversion between ß'-IV and ß-VI form and the fat bloom formation. This work indicates that the monitor for crystalline properties of different positions in cocoa butter products helps the chocolate industry to control formation of fat bloom.


Assuntos
Cacau , Chocolate , Chocolate/análise , Cristalização , Gorduras na Dieta/análise
2.
Oxid Med Cell Longev ; 2021: 3827501, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34804364

RESUMO

Acute lung injury (ALI) is one of the fatal symptoms of sepsis. However, there were no effective clinical treatments. TF accumulation-induced fibrin deposit formations and coagulation abnormalities in pulmonary vessels contribute to the lethality of ALI. Suppressor of cytokine signaling 3 (SOCS3) acts as an endogenous negative regulator of the TLR4/TF pathway. We hypothesized that inducing SOCS3 expression using lidocaine to suppress the TLR4/TF pathway may alleviate ALI. Hematoxylin and eosin (H&E), B-mode ultrasound, and flow cytometry were used to measure the pathological damage of mice. Gelatin zymography was used to measure matrix metalloproteinase-2/9 (MMP-2/9) activities. Western blot was used to assay the expression of protein levels. Here, we show that lidocaine could increase the survival rate of ALI mice and ameliorate the lung injury of ALI mice including reducing the edema, neutrophil infiltration, and pulmonary thrombosis formation and increasing blood flow velocity. Moreover, in vitro and in vivo, lidocaine could increase the expression of p-AMPK and SOCS3 and subsequently decrease the expression of p-ASK1, p-p38, TF, and the activity of MMP-2/9. Taken together, our study demonstrated that lidocaine could inhibit the TLR4/ASK1/TF pathway to alleviate ALI via activating AMPK-SOCS3 axis.

3.
Sci Total Environ ; : 151773, 2021 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-34808164

RESUMO

Reptiles are sensitive to temperature changes as ectotherm animals. The climate warming may pose more serious threat to reptiles. Although the behavior effect and reproduction biology have been well studied, little information is available about the adaptation mechanisms of reptiles to temperature stress. In this study, the native Chinese species, Eremias argus were incubated at 15 (cold stress), 25 (control group) and 35 °C (thermal stress) for 24 h. The transcriptome and metabolome technology were applied to investigate the molecular regulation mechanisms of lizards to acute temperature changes. The CIRBP and HSPA8 were hub genes in response to temperature adaptation. The increased expression of PER gene in lizard circadian rhythm is associated with tyrosine metabolism after cold or thermal stress. The poly-unsaturated fatty acids in female lizard liver were significantly increased with up-regulation of FASN and ACACA genes after thermal stress, which proved the disruption of fatty acid biosynthesis pathway in corporation with the altered body weight. The cortisol and testosterone were important steroid hormones in response to temperature changes especially in male lizard liver. The increased CIRBP gene expression in lizard gonads suppressed the KDM6B gene, which regulates the testis development and may induce sex reversal in male lizard after thermal stress. The adaptation responses of lizards to temperature stress may threaten the health status of wild population.

4.
J Am Chem Soc ; 143(41): 17004-17014, 2021 10 20.
Artigo em Inglês | MEDLINE | ID: mdl-34623792

RESUMO

Rapid screening of infected individuals from a large population is an effective means in epidemiology, especially to contain outbreaks such as COVID-19. The gold standard assays for COVID-19 diagnostics are mainly based on the reverse transcription polymerase chain reaction, which mismatches the requirements for wide-population screening due to time-consuming nucleic acid extraction and amplification procedures. Here, we report a direct nucleic acid assay by using a graphene field-effect transistor (g-FET) with Y-shaped DNA dual probes (Y-dual probes). The assay relies on Y-dual probes modified on g-FET simultaneously targeting ORF1ab and N genes of SARS-CoV-2 nucleic acid, enabling high a recognition ratio and a limit of detection (0.03 copy µL-1) 1-2 orders of magnitude lower than existing nucleic acid assays. The assay realizes the fastest nucleic acid testing (∼1 min) and achieves direct 5-in-1 pooled testing for the first time. Owing to its rapid, ultrasensitive, easily operated features as well as capability in pooled testing, it holds great promise as a comprehensive tool for population-wide screening of COVID-19 and other epidemics.


Assuntos
Sondas de DNA , DNA Viral/análise , Técnicas de Amplificação de Ácido Nucleico/métodos , SARS-CoV-2/genética , COVID-19/diagnóstico , COVID-19/virologia , Grafite/química , Humanos , Limite de Detecção
5.
J Ethnopharmacol ; 284: 114671, 2021 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-34627983

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Aralia, which belongs to Araliaceae family, is mainly distributed in Asia, such as China, Japan and South Korea. It has a long medicinal history and is widely used in the treatment of various diseases, such as hepatitis, rheumatoid arthritis, bruises, lumps and carbuncles. AIM OF THE STUDY: The purpose of this review is to systematically evaluate the traditional uses, phytochemistry, pharmacology, toxicity and quality control of main medicinal plants of Aralia, discusses the application of ethnic medicine, modern scientific research and the relationship between them, and put forward some suggestions to promote the further development and utilization of Aralia. MATERIALS AND METHODS: The relevant information on Aralia was collected through electronic databases (PubMed, Web of Science, Science Direct, Springer, CNKI and Wanfang), Chinese herbal classics, Ph.D. and M.Sc. dissertations, Chinese Pharmacopoeia. Plant names were verified by "The Plant List" (http://www.theplantlist.org). The literature cited in this review can be traced back to 1878 to 2021. RESULTS: More than 290 chemical constituents have been isolated from the genus Aralia, including triterpenoid saponins, terpenoids, organic acids, flavonoids, polyacetylenes, phenylpropanoids and other constituents. Pharmacological studies have shown that the extracts and compounds of Aralia have a wide range of pharmacological activities, including anti-inflammation, analgesic, anti-tumor, liver protection, protection of cardiovascular and nervous system, regulating substance metabolism, antibacterial, antiviral and antioxidation. CONCLUSIONS: The genus Aralia is not only an excellent traditional herbal medicine, but also a source of bioactive molecules with good application prospects. However, the structure-activity relationship, in vivo activity and action mechanism of its bioactive components need to be further studied. In addition, more toxicological and quality control studies are essential to evaluate the efficacy and safety of Aralia as medicine.

6.
Int Immunopharmacol ; 101(Pt B): 108239, 2021 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-34653728

RESUMO

At present, inflammatory bowel disease (IBD) seriously threatens human health, and its treatment is a huge challenge for people. In our studies, we found that meisoindigo, a derivative of indirubin, significantly ameliorated dextran sulfate sodium (DSS)-induced experimental colitis in mice. Meisoindigo treatment markedly elevated the level of glutathione, while suppressed the activities of alkaline phosphatase and myeloperoxidase in colonic tissues. Moreover, the mRNA expression of vascular cell adhesion molecule 1, intercellular adhesion molecule 1, cyclooxygenase-2 which are important colitis-related molecules and the levels of the inflammatory cytokines interleukin (IL)-18, IL-1ß, IL-6, tumor necrosis factor (TNF)-α and inducible nitric oxide synthase (iNOS) were suppressed dose-dependently following treatment with meisoindigo. Immunofluorescence results indicated that meisoindigo inhibited macrophage infiltration and nuclear factor (NF)-κB activation in colons from DSS-treated mice. Therefore, mouse RAW264.7 and human THP-1 cells were treated with lipopolysaccharide (LPS) alone or combined adenosine triphosphate to activate NF-κB pathway in vitro. It was shown that meisoindigo reduced the elevated levels of NO, IL-18, IL-1ß and TNF-α after LPS treatment in both cells. In addition, meisoindigo showed inhibitory effects on NF-κB by using a luciferase reporter gene that depends on NF-κB. Through molecular docking, microscale thermophoresis and cellular thermal shift assay. It was further found that meisoindigo targeted transforming growth factor ß activated kinase-1 (TAK1), which is an important regulator in the upstream of NF-κB pathway. In conclusion, our findings show that meisoindigo can alleviate IBD effectively at low doses, and negatively regulate proinflammatory responses by inhibiting the activation of TAK1, which provides new ideas for clinical anti-inflammatory therapy.

7.
ACS Appl Mater Interfaces ; 13(36): 43426-43437, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34491715

RESUMO

The fabrication of a single polymer network that exhibits a good reversible two-way shape memory effect (2W-SME), can be formed into arbitrarily complex three-dimensional (3D) shapes, and is recyclable remains a challenge. Herein, we design and fabricate poly(thiourethane) (PTU) networks with an excellent thermadapt reversible 2W-SME, arbitrary reconfigurability, and good recyclability via the synergistic effects of multiple dynamic covalent bonds (i.e., ester, urethane, and thiourethane bonds). The PTU samples with good mechanical performance simultaneously demonstrate a maximum tensile stress of 29.7 ± 1.1 MPa and a high strain of 474.8 ± 7.5%. In addition, the fraction of reversible strain of the PTU with 20 wt % hard segment reaches 22.4% during the reversible 2W-SME, where the fraction of reversible strain is enhanced by self-nucleated crystallization of the PTU. A sample with arbitrarily complex permanent 3D shapes can be realized via the solid-state plasticity, and that sample also exhibits excellent reversible 2W-SME. A smart light-responsive actuator with a double control switch is fabricated using a reversible two-way shape memory PTU/MXene film. In addition, the PTU networks are de-cross-linked by alcohol solvolysis, enabling the recovery of monomers and the realization of recyclability. Therefore, the present study involving the design and fabrication of a PTU network for potential applications in intelligent actuators and multifunctional shape-shifting devices provides a new strategy for the development of thermadapt reversible two-way shape memory polymers.

8.
Ecotoxicol Environ Saf ; 225: 112718, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34478984

RESUMO

As a common environmental pollutant, nickel chloride (NiCl2) poses serious threat to human and animals health. NiCl2 has adverse effects on reproductive function in male, however, the underlying mechanisms are not fully illuminated. In this study, 64 male ICR mice were divided into four groups (8 mice per each period/ group), in which mice orally administrated with 0, 7.5, 15 or 30 mg/kg body weight for 14 or 28 consecutive days, respectively. The results showed that the sperm concentration (12.95%, 29.78% and 37.63% -) and sperm motility (19.79%, 34.88% and 43.10%) were dose-dependent significantly reduced, and the total sperm malformation rates (110.15%, 206.84% and 292.27%) were dose-dependent significantly elevated in the 7.5, 15 and 30 mg/kg NiCl2 treatment groups (vs control at 28 days), respectively (P < 0.05). Meanwhile, NiCl2 also decreased the relative weights of testis and epididymis and caused histopathological lesions of testis and epididymis. Furthermore, serum testosterone levels were significantly decreased after NiCl2 treatment. And the findings showed that NiCl2 down-regulated the expression of LH-R, StAR, P450scc, 3ß-HSD, 17ß-HSD, ABP and INHßB in the testis, however, the relative genes in the hypothalamus (Kiss-1, GPR54 and GnRH) and pituitary (GnRH-R, LHß and FSHß) did not exhibit noticeable change. In summary, NiCl2 induced spermatogenesis disorder by testicular damage and hypothalamic-pituitary-testis axis disruption in mice, and only impaired the genes on the testis of HPT axis.


Assuntos
Motilidade Espermática , Testículo , Animais , Masculino , Camundongos , Camundongos Endogâmicos ICR , Níquel , Espermatogênese , Testosterona
9.
Curr Eye Res ; 2021 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-34547966

RESUMO

PURPOSE: To assess the retinal neurodegeneration in type-1 diabetes mellitus (T1DM) and type-2 diabetes mellitus (T2DM) rhesus monkeys, and to investigate whether alterations of glial cells occur in the early stage of diabetic retinopathy (DR). MATERIAL AND METHODS: T1DM rhesus monkeys were established by daily intravenous injections of streptozotocin (STZ, 25 mg/kg body weight) in citrate buffer (pH 4.5) for 5 days, while T2DM rhesus monkeys were induced by feeding with high-fat diet. The period of DR in rhesus monkeys was evaluated by fundoscopy and optical coherence tomography (OCT). Afterward, the morphological changes of inner neurons and glial cells in the retina were detected by immunofluorescence (IF). RESULTS: When compared with the control groups, no difference was observed in both T1DM and T2DM by fundus photographs, while slight exudation and effusion in the blood vessels of retina of rhesus monkeys were found by OCT in DM rhesus monkeys. In addition, the expression of glial fibrillary acidic protein (GFAP) and ionized calcium-binding adaptor molecule (Iba1) were significantly increased in both T1DM (P<0.01) and T2DM (P<0.05) rhesus monkeys. Moreover, the positive expression of PKC-α, parvalbumin and NeuN were significantly decreased, while the positive expression of calbindin showed no difference in T1DM group. However, only the expression cells of PKC-α were reduced in T2DM group when compared with that of the control group. CONCLUSION: Astrocytes activation, reactive gliosis, and neurodegeneration were observed in both T1DM and T2DM rhesus monkey models at the early stage of DR.

10.
Front Oncol ; 11: 718556, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34497768

RESUMO

Background: For locally advanced gastric cancer (LAGC) with serosal invasion (cT4NxM0), adjuvant chemotherapy (AC) after D2 gastrectomy is the standard therapy in Asia. However, perioperative chemotherapy (PCT) combined with D2 gastrectomy is mostly suggested in Europe and America. As a part of PCT, the value of neoadjuvant chemotherapy (NAC) is unclear. We investigated whether NAC could further improve survival and other outcomes for these patients. Methods: Patients with cT4NxM0 gastric cancer who underwent D2 gastrectomy were analyzed. The patients were divided into two groups based on whether they received NAC: the neoadjuvant chemotherapy (NAC) and direct surgery (S) groups. After propensity score matching (1:1 ratio), survival and perioperative outcomes were analyzed between the two groups. Results: A total of 902 patients met all the eligibility criteria and were enrolled. After propensity score matching, 221 matched pairs of patients were identified. The median overall survival (OS) and disease-free survival (DFS) of all patients were 75.10 and 43.67 months, respectively. The median OS of patients in the NAC and S groups were undefined and 29.80 months, respectively (P<0.0001). The median DFS of patients in the NAC and S groups were undefined and 22.60 months (P<0.0001). There were no significant differences in the radical degrees of operation between the two groups (P=0.07). However, there were significant differences in postoperative hospital stay (P<0.001) and complications (P=0.037) between the two groups. Conclusion: This study suggested NAC can further improve prognosis and prevent recurrence in LAGC (cT4NxM0) patients. NAC is feasible and safe for LAGC (cT4NxM0) patients, and does not increase the risk of perioperative surgery.

11.
Macromol Rapid Commun ; : e2100499, 2021 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-34480782

RESUMO

Stimulus-responsive hydrogels are of great significance in soft robotics, wearable electronic devices, and sensors. Near-infrared (NIR) light is considered an ideal stimulus as it can trigger the response behavior remotely and precisely. In this work, a smart flexible stimuli-responsive hydrogel with excellent photothermal property and decent conductivity are prepared by incorporating MXene nanosheets into the physically cross-linked poly(N-isopropyl acrylamide) hydrogel matrix. Because of outstanding photothermal effect and dispersion of MXene, the composite hydrogel exhibits rapid photothermal responsiveness and excellent photothermal stability under the NIR irradiation. Furthermore, the anisotropic bilayer hydrogel actuator shows fast and controllable light-driven bending behavior, which can be used as a light-controlled soft manipulator. Meanwhile, the hydrogel sensor exhibits cycling stability and good durability in detecting various deformation and real-time human activities. Therefore, the present study involving the fabrication of MXene nanocomposite hydrogels for potential applications in remotely controlled actuator and wearable electronic device provides a new method for the development of photothermal responsive conductive hydrogels.

12.
Front Oncol ; 11: 709617, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34540676

RESUMO

Background: For gastric cancer (GC) with extensive lymph node metastasis (bulky N2 and/or para-aortic lymph node metastases), there is no standard therapy worldwide. In Japan, preoperative chemotherapy (PCT) followed by D2 gastrectomy plus para-aortic lymph node dissection (PAND) is considered the standard treatment for these patients. However, in China, the standard operation for GC patients with only bulky N2 metastases was D2 gastrectomy. Besides, after PCT, whether doing PAND improves survival or not is debatable for GC patients with para-aortic lymph node (PAN) metastases. Therefore, we conducted this study to investigate whether D2 lymphadenectomy alone is suitable for these patients after PCT. Methods: We retrospectively collected data on patients from our electronic medical record system. GC patients with bulky N2 and/or PAN metastases who underwent D2 lymphadenectomy alone after PCT were enrolled. The survival outcomes and chemotherapy responses were analyzed and compared with the results of the JCOG0405 study. Results: From May 2009 to December 2017, a total of 83 patients met all eligibility criteria and were enrolled. The median survival duration for all patients was 40.0 months. The 3-year and 5-year OS rates for all patients were 50.3% and 45.6%, respectively. For patients with only bulky N2 metastasis, the 3-year and 5-year OS rates were 77.1% and 71.6%, respectively, which were similar to the results of the JCOG0405 study (82.7% and 73.4%). For patients with only PAN metastases, the 3-year and 5-year OS rates were 50.0% and 50.0%, respectively, which seemed to be lower than those of the JCOG0405 study (64.3% and 57.1%). For patients with bulky N2 and PAN metastases, the 3-year and 5-year OS rates were 7.4% and 0.0%, respectively, which were lower than those of the JCOG0405 study (20.0% and 20.0%). Conclusion: The results of our study suggest that D2 lymphadenectomy alone is suitable for GC patients with only bulky N2 metastasis after PCT. However, D2 lymphadenectomy alone perhaps is not suitable for patients with bulky N2 and PAN metastases after PCT.

13.
Small ; 17(39): e2102710, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34418294

RESUMO

Lithium-sulfur batteries are one of the most promising next-generation energy storage systems. The efficient interconversion between sulfur/lithium polysulfides and lithium sulfide is a performance-determining factor for lithium-sulfur batteries. Herein, a novel strategy to synthesize a unique tube-in-tube CNT-wired sulfur-deficient MoS2 nanostructure embedding cobalt atom clusters as an efficient polysulfide regulator is successfully conducted in Li-S batteries. It is confirmed that encapsulating MWCNTs into hollow porous sulfur-deficient MoS2 nanotubes embedded with metal cobalt clusters not only can accelerate electron transport and confine the dissolution of lithium polysulfide by physical/chemical adsorption, but also can catalyze the kinetics of polysulfide redox reactions. Based on DFT calculations, in situ spectroscopic techniques, and various electrochemical studies, catalytic effects of CNT/MoS2 -Co nanocomposite in Li-S battery are deeply investigated for the first time. The CNT/MoS2 -Co composite cathode exhibits a very remarkable rate capability (641 mAh g-1 at 5.0 C) and excellent cycling stability (capacity decay rate of 0.050% per cycle at 5.0 C) even at high sulfur mass loading of 3.6 mg cm-2 . More crucially, CNT/MoS2 -Co tube-in-tube nanostructures present a superior specific capacity of 650 mAh g-1 in a Li-S pouch cell at 0.2 C (4.0 mg cm-2 ).

14.
Annu Rev Vis Sci ; 7: 437-464, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34348034

RESUMO

Image quality assessment (IQA) models aim to establish a quantitative relationship between visual images and their quality as perceived by human observers. IQA modeling plays a special bridging role between vision science and engineering practice, both as a test-bed for vision theories and computational biovision models and as a powerful tool that could potentially have a profound impact on a broad range of image processing, computer vision, and computer graphics applications for design, optimization, and evaluation purposes. The growth of IQA research has accelerated over the past two decades. In this review, we present an overview of IQA methods from a Bayesian perspective, with the goals of unifying a wide spectrum of IQA approaches under a common framework and providing useful references to fundamental concepts accessible to vision scientists and image processing practitioners. We discuss the implications of the successes and limitations of modern IQA methods for biological vision and the prospect for vision science to inform the design of future artificial vision systems. (The detailed model taxonomy can be found at http://ivc.uwaterloo.ca/research/bayesianIQA/.).

15.
Macromol Biosci ; 21(11): e2100262, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34449122

RESUMO

Bone-like apatite is a promising coating of poly(ether ether ketone) (PEEK) for bone implantation. Poly(aryl ether nitrile ketone) containing phthalazinone moiety (PPENK) is a novel alternative for its easy synthesis. Here, chitosan/gelatin hybrid hydrogel coating is applied to induce the formation of apatite on the surface of PPENK substrate through biomineralization to improve its biocompatibility and osteogenic property. PPENK possessing allyl groups (PPENK-d) are synthesized and spin-coated on PPENK substrate to impart reactive groups. The hydrogel coating is prepared by the ultraviolet crosslinking of gelatin methacrylate (GelMA) and chitosan methacrylate (CSMA) on PPENK substrate. PPENK-d, GelMA, and CSMA are characterized by 1 H-NMR to confirm the designed structures. The presence of chitosan increases the chelation of calcium ions and thus induces the nucleation of apatite. The microstructural and compositional results reveal that the chitosan-containing hydrogel coating induced apatite coating yields a higher apatite quantity compared to the gelatin hydrogel coating. The apatite coatings on PPENK substrate promote the cytocompatibility and osteogenesis of MC3T3-E1 preosteoblasts in vitro.

16.
Front Immunol ; 12: 609615, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34322115

RESUMO

Background: Rosacea, a chronic inflammatory skin disorder etiologically associated with immune cells and the antibacterial peptide cathelicidin LL-37, can be effectively treated by oral carvedilol administration. Objective: To investigate the molecular mechanisms underlying carvedilol efficacy in rosacea treatment. Methods: Skin samples of patients with rosacea were subjected to histopathological (hematoxylin and eosin) and immunohistochemical (CD68, Toll-like receptor 2 (TLR2), kallikrein 5, cathelicidin, TNF-α, and IL-1ß) evaluation. An in vivo murine rosacea-like inflammation model was established by LL-37 intradermal injection with or without carvedilol gavage-based pretreatment. Erythema proportion (Image J) and skin redness (L*a*b colorimetry) were quantified. Murine skin samples underwent pathological examination for inflammatory status and immunofluorescence staining. Murine skin and lipopolysaccharide-stimulated RAW 264.7 cells with or without carvedilol pretreatment were evaluated by quantitative reverse transcription-polymerase chain reaction and western blotting. Clinical facial images of patients were obtained using the VISIA skin analysis system before, 4, and 6 months following oral carvedilol administration. Results: Rosacea skin lesions exhibited more pronounced inflammatory cell infiltration than peripheral areas, with profound macrophage infiltration and inflammatory cytokines (TLR2, kallikrein 5, cathelicidin, TNF-α, and IL-1ß). In vivo, carvedilol alleviated inflammation in LL-37 mice, down-regulating TLR2, KLK5, and cathelicidin expression. In vitro, carvedilol decreased TLR2 expression in RAW 264.7 cells, further reducing KLK5 secretion and LL-37 expression and ultimately inhibiting rosacea-like inflammatory reactions. Clinical manifestations and facial redness obviously improved during 6-month follow-up with systemic carvedilol administration. Conclusion: Carvedilol is effective against rosacea, with inhibition of macrophage TLR2 expression as a novel anti-inflammatory mechanism.


Assuntos
Anti-Inflamatórios/uso terapêutico , Carvedilol/uso terapêutico , Macrófagos/efeitos dos fármacos , Rosácea/tratamento farmacológico , Pele/efeitos dos fármacos , Receptor 2 Toll-Like/antagonistas & inibidores , Animais , Peptídeos Catiônicos Antimicrobianos/metabolismo , Citocinas/genética , Citocinas/metabolismo , Modelos Animais de Doenças , Feminino , Humanos , Mediadores da Inflamação/metabolismo , Calicreínas/genética , Calicreínas/metabolismo , Macrófagos/imunologia , Macrófagos/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Células RAW 264.7 , Rosácea/imunologia , Rosácea/metabolismo , Rosácea/patologia , Pele/imunologia , Pele/metabolismo , Pele/patologia , Receptor 2 Toll-Like/genética , Receptor 2 Toll-Like/metabolismo , Resultado do Tratamento
17.
ACS Appl Mater Interfaces ; 13(28): 33404-33416, 2021 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-34247475

RESUMO

Stimulus-responsive intelligent hydrogel actuators have highly promising applications in the fields of soft robotics, smart manipulators, and flexible devices. Near-infrared (NIR) light is considered an ideal method to trigger the response behavior remotely and precisely. In order to realize the excellent optical transmittance and photothermal property of NIR-responsive hydrogels at the same time, two-dimensional nonlayered MoO2 nanosheets (2D-MoO2) with excellent photothermal efficiency (62% under an NIR light irradiation of 808 nm), splendid chemistry stability, and low preparation cost are used as photothermal agents and incorporated into the poly(N-isopropylacrylamide) (PNIPAM) hydrogel network, forming the 2D-MoO2/Laponite/PNIPAM ternary nanocomposite hydrogel (TN hydrogel). It is remarkable that compared with the GO and MXene hydrogels with the same agent content (1.0 mg mL-1) and thickness (1 mm) whose transmittance values are only ∼5% at 600 nm, the TN hydrogel shows a similar NIR-responsive temperature, but much higher optical transmittance (∼53%). Besides, of the three hydrogels with similar transmittance, the TN hydrogel shows a much higher NIR-responsive temperature. The TN hydrogel with a low loading of 2D-MoO2 (1.5 mg mL-1) can produce a significant temperature increase of ∼30 °C after the application of 0.8 W cm-2 NIR light irradiation for 15 s. Impressively, the TN hydrogel exhibits excellent anti-fatigue property, keeping a fast response and temperature rise behavior even after 50 times of heating-cooling cycles. The flexibly controllable and reversible deformation is realized by a well-designed bilayer structure even in harsh environments. The transparent and asymmetric bilayer hydrogel is further used as a soft manipulator to capture objects visually and accurately. The NIR light-controlled microvalve based on this composite hydrogel is also demonstrated. This work provides a novel kind of transparent hybrid NIR response hydrogel for the further development of smart, programmable, reversible hydrogel-based actuators and soft robotics.

18.
ACS Appl Mater Interfaces ; 13(29): 34124-34133, 2021 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-34256569

RESUMO

As a highly toxic and corrosive waste gas in the industry, hydrogen sulfide (H2S) usually originates from the utilization of coal, petroleum, and natural gas. The selective catalytic elimination of H2S shows great significance to ensure the safety of industrial processes and health of human beings. Herein, we report efficiently selective oxidation of H2S to elemental sulfur over covalent triazine framework (CTF-1-x, x = 400, 500, 600, 400-600 °C) catalysts. CTF-1-x samples were prepared from polymerization of 1,4-dicyanobenzene to form polyaryl triazine networks under ion solidothermal conditions in the presence of ZnCl2, which acts as both an initiator and a porogen. The resultant CTF-1-x samples possess abundant micro-mesoporosity, large Brunauer-Emmett-Teller (BET) surface areas, and tunable structural base sites with edge amine and graphitic nitrogen characteristics, which were homogeneously decorated onto their frameworks. As a result, CTF-1-x samples act as efficient and long-lived catalysts in selective oxidation of H2S to sulfur under ambient conditions (100% H2S conversion, 100% sulfur selectivity at 180 °C, 12 000 mL/(g·h)), and their activities were superior to those of commercial Fe2O3 and g-C3N4 desulfurization catalysts. Abundant nitrogen structural base sites of CTF-1-x effectively activate the reactants, and abundant micro-mesoporosity facilitates mass transfer in and out of CTF-1-x. The improved design of the nitrogen-doped carbon material for H2S activation and conversion could enhance the development of more active and robust nitrogen-doped carbon catalysts.

19.
Chemosphere ; 285: 131456, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34256203

RESUMO

Nowadays, the emergence of pesticides and its application in agriculture greatly improved the crop quality and food production. However, the resulted ecological problem caused by the widespread pesticide residues attracted more and more attention since the pesticides were harmful to most living organisms. Regulatory agencies such as Environmental Protection Agency (EPA) and European Chemicals Agency (ECHA) stipulated that a comprehensive pesticides risk assessment was essential and also underscored the application of computation method in evaluating pesticides. The present study aimed to use the Quantitative Structure-Activity Relationship (QSAR) method to establish models for quantitatively and qualitatively predicting the toxicity of pesticide against Skeletonema costatum. The regression model was developed using the Genetic Algorithm plus Multiple Linear Regression method and the classification model was established based on the Random Forest algorithm, respectively. Various internal and external validation metrics suggested that the obtained regression model was of good fitness (R2=0.722), robustness (QLOO2=0.653) and external predictive ability (QFn2:0.719-0.776, CCC = 0.878). The classification could correctly predict 79.4% of pesticides in the training set and 69.7% in the validation set. The relatively high sensitivity value of the classification model indicated its good performance in identifying high-toxic pesticides. It could be concluded from the selected modelling descriptors that molecular weight and polarizability impacted the toxicity the most. The atom-type E-state descriptors generally contributed negatively to the pesticide toxicity which verified the negative influence of molecular hydrophilicity. Moreover, the lipophilic, carbon-type, charge related descriptors demonstrated the important influence of lipophilicity and polarity on pesticide toxicity. The models presented in this work could be used to pre-evaluate the toxicity of pesticides within the applicability domain, thus focusing resources on the high-toxic pesticides and assessing the environmental risk of pesticides quickly and economically.


Assuntos
Praguicidas , Relação Quantitativa Estrutura-Atividade , Algoritmos , Modelos Lineares , Praguicidas/toxicidade
20.
Macromol Biosci ; 21(7): e2100078, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34146384

RESUMO

Tissue adhesion to bone implant and osteoblastic differentiation are the key factors to achieve poly(aryl ether ketone) (PAEK) implant osseointegration. However, physical interaction of implant with tissue and hydroxyapatite coating suffers from slow implant tissue integration and lack of long-term stability. In this study, a novel poly(phthalazinone ether sulfone ketone) containing allyl groups (APPBAESK) is coated onto PPBESK sheet for reacting with the allyl groups of the hydrogel coating to enhance its stability. N-Succinimidyl (NHS)-ester activated group and nano-hydroxyapatite (nano-HA) are introduced into the hydrogel synthesized from gelatin methacrylate (GelMA) and acrylic acid to construct a nanocomposite hydrogel coating on PPBESK which is a promising PAEK implant material. The hydrophilicity of the PPBESK sheet is improved by the hydrogel coating. The chemical components of the nanocomposite hydrogel coating are confirmed by X-ray photoelectron spectroscope, Attenuated total reflection infrared, and X-ray powder diffraction. The tissue shear adhesion strength of the hydrogel coating toward pig skin is enhanced due to the synergism of NHS-ester activated group and nano-HA. The osteogenic differentiation of MC3T3-E1 preosteoblasts is promoted by nano-HA in nanocomposite hydrogel coating. Therefore, the bifunctional nanocomposite hydrogel coating provides a great application prospect in the surface modification of PAEK implants in bone tissue engineering.

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